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Reverse Genetic Analysis of Transcription Factor Os Hox9, a Member of Homeobox Family, in Rice 认领 引用 被引量：5
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作者 AI Li-ping SHEN Ao +4 位作者 GAO Zhi-chao LI Zheng-long SUN Qiong-lin LI Ying-ying LUAN Wei-jiang 《Rice science》 2014年第6期312-317,共6页
Homeobox transcription factors participate in the growth and development of plants by regulating cell differentiation, morphogenesis and environmental signal response. To reveal the functions of these transcription fa... Homeobox transcription factors participate in the growth and development of plants by regulating cell differentiation, morphogenesis and environmental signal response. To reveal the functions of these transcription factors in rice, we constructed the RNAi vectors of OsHox9, a member of homeobox family, and analyzed the function of OsHox9 using reverse genetics. The plant height and tillering number of RNAi transgenic plants decreased compared with those of wild-type plants. Reverse transcdption-polymerase chain reaction analysis showed that OsHox9 expression reduced in the transgenic plants with phenotypic variance, whereas that in the transgenic plants without phenotypic variance was similar to that in the wild-type plants. This result suggests that the phenotypes of the transgenic plants were caused by RNAi effects. The tissue-specificity of OsHox9 expression indicated that it was expressed in different organs, with high expression in stem apical medstem and young panicles. Subcellular location of OsHox9 demonstrated that it was localized on the cell membrane. 展开更多
关键词 expression analysis homeobox transcription factor subcellular location reverse genetics rice RNA interference
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右美托咪定对大鼠肝脏缺血再灌注损伤的影响 认领 引用
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作者 张玮琪 宁夏青 +3 位作者 张士霞 艾丽平 李航 倪耀娣 《中国兽医杂志》 CAS 北大核心 2023年第3期56-62,共7页
为了探讨右美托咪定对肝脏缺血再灌注损伤的影响及其可能的分子机制,本试验将45只清洁级成年雄性SD大鼠随机分为空白组、假手术组、损伤组(肝脏缺血再灌注组)、预给药组(缺血前30 min给予右美托咪定)和后给药组(再灌注后30 min给予右美... 为了探讨右美托咪定对肝脏缺血再灌注损伤的影响及其可能的分子机制,本试验将45只清洁级成年雄性SD大鼠随机分为空白组、假手术组、损伤组(肝脏缺血再灌注组)、预给药组(缺血前30 min给予右美托咪定)和后给药组(再灌注后30 min给予右美托咪定)。建立大鼠肝脏缺血再灌注模型,于再灌注后6 h和12 h取样。用全自动生化分析仪检测谷丙转氨酶(ALT)水平;光镜下观察肝细胞的形态学改变;TUNEL法检测肝细胞凋亡率;实时荧光定量PCR(RT-PCR)法检测内质网应激和凋亡相关基因PERK、ATF-6、GRP-78和Caspase-12 mRNA表达水平。结果显示,与空白组相比,损伤组ALT含量极显著升高(P0.05)。结果表明,右美托咪定能有效减轻肝脏缺血再灌注损伤,其机制可能与降低内质网应激有关。 展开更多
关键词 肝脏缺血再灌注 右美托咪定 内质网应激 细胞凋亡
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