Moth bean(Vigna aconitifolia)is a legume rich in proteins,bioactive compounds,and essential nutrients,widely used in traditional diets across Asia.Its proteins possess significant functional and nutritional potential,...Moth bean(Vigna aconitifolia)is a legume rich in proteins,bioactive compounds,and essential nutrients,widely used in traditional diets across Asia.Its proteins possess significant functional and nutritional potential,but conventional extraction methods often limit yield and bioactivity.This study evaluated the impact of microwaveassisted extraction(MAE)on moth bean protein(MMBP)in comparison with native moth bean protein(NMBP).Protein yield improved from 18.46%(NMBP)to 21.43%(MMBP),while protein content increased from 70.90%to 74.85%.Structural analyses showed clear modifications,with Fourier transform infrared spectroscopy revealing shifts in amide I(1571-1663 cm-1)and amide II(1410-1548 cm-1)bands,and Thermogravimetric analysis indicating higher mass loss in MMBP.Particle size reduced significantly from 354.22 nm(NMBP)to 261.01 nm(MMBP),while zeta potential increased from18.81 to23.55 mV,reflecting greater stability.Invitro digestibility improved from 76.13%to 82.89%,supported by unfolding-driven exposure of peptide sites.Amino acid content increased from 66.61 to 71.56 g/100 g protein,with notable rises in leucine(4.87-6.02 g/100 g)and valine(3.25-3.92 g/100 g).Bioactivity was enhanced,with higher phenolics(2.89-3.95 mg GAE/g),and flavonoids(1.80-2.65 mg QE/g),alongside reduced tannins(1.48-0.93mg CE/g)and phytic acid(4.09-2.03 mg/g).These findings establish MAE as an effective strategy to enhance the functional and nutritional quality of moth bean proteins.展开更多
Dimethyl phthalate(DMP)is a commonly used plasticizer incorporated into food packaging materials to enhance flexibility,however,its propensity to migrate into the environment has raised significant concerns regarding ...Dimethyl phthalate(DMP)is a commonly used plasticizer incorporated into food packaging materials to enhance flexibility,however,its propensity to migrate into the environment has raised significant concerns regarding environmental contamination and human exposure through water and food.Therefore,the present study explores the synthesis of rice husk-derived carbon quantum dots(RH-CQDs)for dual DMP sensing and photocatalytic dye degradation.Hydrothermally synthesized RH-CQDs demonstrated high fluorescence with strong emission at 528 nm and an excitation wavelength of 291 nm.Structurally,RH-CQDs confirmed their spherical morphology with a particle size of 6.06±0.08 nm.The RH-CQDs exhibited excellent selectivity and sensitivity towards DMP,enabling rapid detection through fluorescence quenching mechanisms,with a low limit of detection(LOD)of 0.88967μmol/L.High-Performance Liquid Chromatography(HPLC)was used to validate DMP detection,confirming the reliability of the sensing approach.In addition to sensing applications,RH-CQDs exhibited remarkable photocatalytic activity,achieving 74-76% cationic and anionic dye degradation.Addi-tionally,RH-CQDs exhibited potent antimicrobial activity,with a maximum inhibition zone of 26.57±0.04 mm against Staphylococcus aureus,highlighting microbial control potential.Overall,this research underscores the potential of RH-CQDs as a green and scalable nanomaterial for environmental remediation.展开更多
This study developed and characterized nanocomposite films incorporating varying concentrations of nano-cellulose(NC),gum arabic,glycerol,sodium alginate,and eugenol.The mechanical strength analysis identified F6(5%NC...This study developed and characterized nanocomposite films incorporating varying concentrations of nano-cellulose(NC),gum arabic,glycerol,sodium alginate,and eugenol.The mechanical strength analysis identified F6(5%NC,4%gum arabic)as having the highest strength at 25.89±0.32 MPa compared to control films(F-CNC and F-CGA).Moisture content,water activity,and water vapor transmission rate were lowest in F6,measuring 16.41±0.67%0.533±0.03,and 0.45±0.06 kg s-1m2⋅Pa-1respectively,indicating enhanced water retention and barrier properties.Thermal stability tests showed F-NC had superior resistance,with 95.23%degradation at 556.65℃.Surface morphology revealed a smooth and uniform surface for F-NC,while F-CNC and F-CGA displayed rough textures and irregular clusters.FTIR analysis confirmed significant interactions among components,indicated by O-H and C-H stretching bands.Biodegradability assessments demonstrated that F-NC fully decomposed by Day 25.The F-NC film displayed the lowest microbial counts,with a MIC of 0.51±0.37 and MBC of 0.41±0.29.Further,the physicochemical prosperities of bread samples at the intervals of 0,2,4,6,and 8 days.The bread samples exhibited 22%moisture content,0.754 water activity,and 250N hardness.Overall,F-NC films exhibit exceptional mechanical,thermal,and environmental properties,suitable for sus-tainable packaging.展开更多
This study aimed to utilize the Cordia dichotoma fruit nanomucilage(CDM)and beetroot anthocyanin(BA)derived functional and antimicrobial nanoemulsion for the post-harvest management of tomatoes.CDM was extracted using...This study aimed to utilize the Cordia dichotoma fruit nanomucilage(CDM)and beetroot anthocyanin(BA)derived functional and antimicrobial nanoemulsion for the post-harvest management of tomatoes.CDM was extracted using hot water extraction and the yield of spray-dried mucilage powder(SDMP)was 13.44±0.94%,with a particle size of 115.73±3.58 nm and a zeta potential of-26.11±0.85 mV.Nanoemulsion was prepared using different concentrations of BA(1-5%w/v)and flaxseed oil(1-4%v/v)using a low-energy method.The selection of nanoemulsion was carried out based on droplet size,creaming index,and antimicrobial activity.Results revealed that nanoemulsion prepared using 1%flaxseed oil and 3%BA(CDM-OA13),2%flaxseed oil,and 3%BA(CDM-OA23)showed higher stability than other samples.The CDM-OA23 showed higher antimicrobial activity compared to CDM-OA13.The shelf-life of nanoemulsion-coated tomatoes(CDM-OA23)and uncoated tomatoes(UC-T)were studied over the 15-day storage period.Results showed that tomatoes coated with CDM-OA23 nanoemulsion reduced the weight loss(19.43±0.64%)compared to the control sample(27.29±0.73%)on the 15th day.The CDM-OA23 nanoemulsion coating maintained the titrable acidity(TSS),Total soluble solids(TSS),Total phenolic content(TPC),Total Flavanoid content(TFC)and firmness of tomatoes over the 15 days of storage period.Thus,CDM and BA-infused nanoemulsion potentially enhanced the shelf-life of tomatoes by protecting food pathogenic microbes and maintaining the quality of the tomatoes over 15 days of storage.展开更多
This study aimed to utilize the different plant-derived mucilage sources for the partial replacement of palm oil in compound chocolate.Colocasia esculenta rhizome mucilage(CEM),Cordia dichotoma fruit mucilage(CDM),and...This study aimed to utilize the different plant-derived mucilage sources for the partial replacement of palm oil in compound chocolate.Colocasia esculenta rhizome mucilage(CEM),Cordia dichotoma fruit mucilage(CDM),and Psyllium husk mucilage(PHM)were extracted using suitable green methods.Spray-dryer was used to obtain the uniform mucilage powder and the yield of spray-dried CEM(4.65±0.79%),CDM(13.44±0.27%),and PHM(48.12±0.86%)were calculated on a dry basis.Furthermore,different ratios of the mucilage and palm oil(0-100%)were used for the preparation of chocolate spread.CEM30(30%CEM and 70%palm oil)showed similar viscosity compared to the control(CEMC).The selected compound chocolate was stored for 120 days and physicochemical data revealed a non-significant(p<0.05)difference throughout 120 days of storage.CEMC exhibited 516.75±0.27 kcal/100 g energy values which was higher than CEM30(485.24±0.77 kcal/100 g)on the 0th day.Differential calorimetry(DSC)data confirmed the similar melting behavior around 37℃ for both samples.On the 120th day,CEM30 displayed a similar texture as CEMC.The L*value of CEMC showed 48.54±0.42 and for CEM30 it was 46.89±0.58 on the 0th day.Overall,CEM30 has proven the most suitable formulation that can be effectively used as a partial substitute for palm oil as a cost-effective natural ingredient in the chocolate industry.展开更多
Algae,particularly Chlorella vulgaris,present a novel,sustainable source for producing carbon quantum dots(CQDs)with unique properties.This study introduces an eco-friendly synthesis method using a hydrothermal proces...Algae,particularly Chlorella vulgaris,present a novel,sustainable source for producing carbon quantum dots(CQDs)with unique properties.This study introduces an eco-friendly synthesis method using a hydrothermal process at 200℃ for 12 h,yielding stable,spherical CQDs with an average diameter of 7.19±0.06 nm,composed primarily of carbon and oxygen.The innovation consists on employing microalgal biomass for the production of CQDs,hence obviating the necessity for additional chemicals or passivating agents.The CQDs exhibit a fluorescence peak at 416 nm upon excitation at 241 nm,with UV-visible spectra showing π→π* and n→π* transition at 241 nm and 356 nm,respectively.High-resolution TEM analysis reveals a crystalline structure with a 0.21 nm interlayer spacing,confirmed by lattice fringes,and a quantum yield of 41.24%,indicating efficient photoluminescence.The CQDs demonstrate strong antimicrobial activity against pathogens like Staphylococcus aureus and Pseudomonas aeruginosa,with significant inhibition of quorum sensing like twitching and swarming and bacterial motilities.Furthermore,the CQDs achieve a 91.47% degradation of malachite green dye,underscoring their potential in environmental remediation.This study highlights the dual applications of Chlorella vulgaris-derived CQDs in health and environmental contexts,presenting a sustainable and innovative approach for nanomaterial synthesis.展开更多
Whey is the primary waste material of the dairy industries;therefore,the present study focuses on the valorization of milk processing industry-originated liquid whey into valuable products,specifically liquid whey car...Whey is the primary waste material of the dairy industries;therefore,the present study focuses on the valorization of milk processing industry-originated liquid whey into valuable products,specifically liquid whey carbon quantum dots(LW-CQDs)for pathogenic bactericidal and photocatalytic dye reduction efficacy.Facile synthesis of fluorescent LW-CQDs was carried out by employing a green hydrothermal approach at 200℃ for 12 h.Several analytical techniques were used to confirm the thermally stable spherical particles,measuring 9.04±0.76 nm size in diameter,primarily composed of carbon and oxygen LW-CQDs.The presence of diverse functional groups(hydroxyl,carboxyl,carbonyl,and methyl groups)of LW-CQDs contributed to overall optical properties,which manifested a blue emission peak at 418 nm with 240 nm excitation wavelength in fluorescence spectroscopy.UV-Visible spectra featured two peaks(π→π*transition of C=C bonds and n→π*transition of C=O bonds)at around 249 and 293 nm,respectively.Moreover,the synthesized LW-CQDs exhibited a significantly higher zone of inhibition(25.98±0.17 mm)and significantly lower minimum inhibitory concentration(4.47±0.01μl/ml)against the Staphylococcus aureus.In addition,LW-CQDs revealed a higher killing rate of reaction for S.aureus as compared to K.pneumoniae,P.aeruginosa,and S.typhi and remarkably degraded 92.95%of brilliant red dye under visible light(2000 lux).Hence,these facile LW-CQDs hold potential for applications in the effectiveness of antimicrobial and photocatalytic dye reduction activity,which show valuable contributions to both waste valorization and sustainable material development.展开更多
基金Funding Program(ORF-2026-940),King Saud University,Riyadh,Saudi Arabia,for supporting this study.
摘要Moth bean(Vigna aconitifolia)is a legume rich in proteins,bioactive compounds,and essential nutrients,widely used in traditional diets across Asia.Its proteins possess significant functional and nutritional potential,but conventional extraction methods often limit yield and bioactivity.This study evaluated the impact of microwaveassisted extraction(MAE)on moth bean protein(MMBP)in comparison with native moth bean protein(NMBP).Protein yield improved from 18.46%(NMBP)to 21.43%(MMBP),while protein content increased from 70.90%to 74.85%.Structural analyses showed clear modifications,with Fourier transform infrared spectroscopy revealing shifts in amide I(1571-1663 cm-1)and amide II(1410-1548 cm-1)bands,and Thermogravimetric analysis indicating higher mass loss in MMBP.Particle size reduced significantly from 354.22 nm(NMBP)to 261.01 nm(MMBP),while zeta potential increased from18.81 to23.55 mV,reflecting greater stability.Invitro digestibility improved from 76.13%to 82.89%,supported by unfolding-driven exposure of peptide sites.Amino acid content increased from 66.61 to 71.56 g/100 g protein,with notable rises in leucine(4.87-6.02 g/100 g)and valine(3.25-3.92 g/100 g).Bioactivity was enhanced,with higher phenolics(2.89-3.95 mg GAE/g),and flavonoids(1.80-2.65 mg QE/g),alongside reduced tannins(1.48-0.93mg CE/g)and phytic acid(4.09-2.03 mg/g).These findings establish MAE as an effective strategy to enhance the functional and nutritional quality of moth bean proteins.
基金supported and funded by the Deanship of Scientific Research at Imam Mohammad Ibn Saud Islamic University(IMSIU)(grant number IMSIU-DDRSP2501).
摘要Dimethyl phthalate(DMP)is a commonly used plasticizer incorporated into food packaging materials to enhance flexibility,however,its propensity to migrate into the environment has raised significant concerns regarding environmental contamination and human exposure through water and food.Therefore,the present study explores the synthesis of rice husk-derived carbon quantum dots(RH-CQDs)for dual DMP sensing and photocatalytic dye degradation.Hydrothermally synthesized RH-CQDs demonstrated high fluorescence with strong emission at 528 nm and an excitation wavelength of 291 nm.Structurally,RH-CQDs confirmed their spherical morphology with a particle size of 6.06±0.08 nm.The RH-CQDs exhibited excellent selectivity and sensitivity towards DMP,enabling rapid detection through fluorescence quenching mechanisms,with a low limit of detection(LOD)of 0.88967μmol/L.High-Performance Liquid Chromatography(HPLC)was used to validate DMP detection,confirming the reliability of the sensing approach.In addition to sensing applications,RH-CQDs exhibited remarkable photocatalytic activity,achieving 74-76% cationic and anionic dye degradation.Addi-tionally,RH-CQDs exhibited potent antimicrobial activity,with a maximum inhibition zone of 26.57±0.04 mm against Staphylococcus aureus,highlighting microbial control potential.Overall,this research underscores the potential of RH-CQDs as a green and scalable nanomaterial for environmental remediation.
基金the Researchers Supporting Project Number(RSPD2025R713)King Saud University,Riyadh,Saudi Arabia for supporting this study.
摘要This study developed and characterized nanocomposite films incorporating varying concentrations of nano-cellulose(NC),gum arabic,glycerol,sodium alginate,and eugenol.The mechanical strength analysis identified F6(5%NC,4%gum arabic)as having the highest strength at 25.89±0.32 MPa compared to control films(F-CNC and F-CGA).Moisture content,water activity,and water vapor transmission rate were lowest in F6,measuring 16.41±0.67%0.533±0.03,and 0.45±0.06 kg s-1m2⋅Pa-1respectively,indicating enhanced water retention and barrier properties.Thermal stability tests showed F-NC had superior resistance,with 95.23%degradation at 556.65℃.Surface morphology revealed a smooth and uniform surface for F-NC,while F-CNC and F-CGA displayed rough textures and irregular clusters.FTIR analysis confirmed significant interactions among components,indicated by O-H and C-H stretching bands.Biodegradability assessments demonstrated that F-NC fully decomposed by Day 25.The F-NC film displayed the lowest microbial counts,with a MIC of 0.51±0.37 and MBC of 0.41±0.29.Further,the physicochemical prosperities of bread samples at the intervals of 0,2,4,6,and 8 days.The bread samples exhibited 22%moisture content,0.754 water activity,and 250N hardness.Overall,F-NC films exhibit exceptional mechanical,thermal,and environmental properties,suitable for sus-tainable packaging.
基金support of Central Instrument Facility,Lovely Professional Universitythe Researchers Supporting Project Number(RSPD2025R1038),King Saud University,Riyadh,Saudi Arabia for supporting this study.
摘要This study aimed to utilize the Cordia dichotoma fruit nanomucilage(CDM)and beetroot anthocyanin(BA)derived functional and antimicrobial nanoemulsion for the post-harvest management of tomatoes.CDM was extracted using hot water extraction and the yield of spray-dried mucilage powder(SDMP)was 13.44±0.94%,with a particle size of 115.73±3.58 nm and a zeta potential of-26.11±0.85 mV.Nanoemulsion was prepared using different concentrations of BA(1-5%w/v)and flaxseed oil(1-4%v/v)using a low-energy method.The selection of nanoemulsion was carried out based on droplet size,creaming index,and antimicrobial activity.Results revealed that nanoemulsion prepared using 1%flaxseed oil and 3%BA(CDM-OA13),2%flaxseed oil,and 3%BA(CDM-OA23)showed higher stability than other samples.The CDM-OA23 showed higher antimicrobial activity compared to CDM-OA13.The shelf-life of nanoemulsion-coated tomatoes(CDM-OA23)and uncoated tomatoes(UC-T)were studied over the 15-day storage period.Results showed that tomatoes coated with CDM-OA23 nanoemulsion reduced the weight loss(19.43±0.64%)compared to the control sample(27.29±0.73%)on the 15th day.The CDM-OA23 nanoemulsion coating maintained the titrable acidity(TSS),Total soluble solids(TSS),Total phenolic content(TPC),Total Flavanoid content(TFC)and firmness of tomatoes over the 15 days of storage period.Thus,CDM and BA-infused nanoemulsion potentially enhanced the shelf-life of tomatoes by protecting food pathogenic microbes and maintaining the quality of the tomatoes over 15 days of storage.
基金the support of Central Instrument Facility,Lovely Professional University.Also,authors acknowledge&extend their appreciation to the Researchers Supporting Project Number(RSPD2024R940),King Saud University,Riyadh,Saudi Arabia for sup-porting this study.
摘要This study aimed to utilize the different plant-derived mucilage sources for the partial replacement of palm oil in compound chocolate.Colocasia esculenta rhizome mucilage(CEM),Cordia dichotoma fruit mucilage(CDM),and Psyllium husk mucilage(PHM)were extracted using suitable green methods.Spray-dryer was used to obtain the uniform mucilage powder and the yield of spray-dried CEM(4.65±0.79%),CDM(13.44±0.27%),and PHM(48.12±0.86%)were calculated on a dry basis.Furthermore,different ratios of the mucilage and palm oil(0-100%)were used for the preparation of chocolate spread.CEM30(30%CEM and 70%palm oil)showed similar viscosity compared to the control(CEMC).The selected compound chocolate was stored for 120 days and physicochemical data revealed a non-significant(p<0.05)difference throughout 120 days of storage.CEMC exhibited 516.75±0.27 kcal/100 g energy values which was higher than CEM30(485.24±0.77 kcal/100 g)on the 0th day.Differential calorimetry(DSC)data confirmed the similar melting behavior around 37℃ for both samples.On the 120th day,CEM30 displayed a similar texture as CEMC.The L*value of CEMC showed 48.54±0.42 and for CEM30 it was 46.89±0.58 on the 0th day.Overall,CEM30 has proven the most suitable formulation that can be effectively used as a partial substitute for palm oil as a cost-effective natural ingredient in the chocolate industry.
基金Support by the Department of Central Instrumentation facility,Lovely Professional University is gratefully acknowledgedthe Researchers Supporting Project Number(RSPD2024R940),King Saud University,Riyadh,Saudi Arabia,for supporting this study.
摘要Algae,particularly Chlorella vulgaris,present a novel,sustainable source for producing carbon quantum dots(CQDs)with unique properties.This study introduces an eco-friendly synthesis method using a hydrothermal process at 200℃ for 12 h,yielding stable,spherical CQDs with an average diameter of 7.19±0.06 nm,composed primarily of carbon and oxygen.The innovation consists on employing microalgal biomass for the production of CQDs,hence obviating the necessity for additional chemicals or passivating agents.The CQDs exhibit a fluorescence peak at 416 nm upon excitation at 241 nm,with UV-visible spectra showing π→π* and n→π* transition at 241 nm and 356 nm,respectively.High-resolution TEM analysis reveals a crystalline structure with a 0.21 nm interlayer spacing,confirmed by lattice fringes,and a quantum yield of 41.24%,indicating efficient photoluminescence.The CQDs demonstrate strong antimicrobial activity against pathogens like Staphylococcus aureus and Pseudomonas aeruginosa,with significant inhibition of quorum sensing like twitching and swarming and bacterial motilities.Furthermore,the CQDs achieve a 91.47% degradation of malachite green dye,underscoring their potential in environmental remediation.This study highlights the dual applications of Chlorella vulgaris-derived CQDs in health and environmental contexts,presenting a sustainable and innovative approach for nanomaterial synthesis.
基金the Researchers Supporting Project Number(RSP2024R379),King Saud University,Riyadh,Saudi Arabia for supporting this study.
摘要Whey is the primary waste material of the dairy industries;therefore,the present study focuses on the valorization of milk processing industry-originated liquid whey into valuable products,specifically liquid whey carbon quantum dots(LW-CQDs)for pathogenic bactericidal and photocatalytic dye reduction efficacy.Facile synthesis of fluorescent LW-CQDs was carried out by employing a green hydrothermal approach at 200℃ for 12 h.Several analytical techniques were used to confirm the thermally stable spherical particles,measuring 9.04±0.76 nm size in diameter,primarily composed of carbon and oxygen LW-CQDs.The presence of diverse functional groups(hydroxyl,carboxyl,carbonyl,and methyl groups)of LW-CQDs contributed to overall optical properties,which manifested a blue emission peak at 418 nm with 240 nm excitation wavelength in fluorescence spectroscopy.UV-Visible spectra featured two peaks(π→π*transition of C=C bonds and n→π*transition of C=O bonds)at around 249 and 293 nm,respectively.Moreover,the synthesized LW-CQDs exhibited a significantly higher zone of inhibition(25.98±0.17 mm)and significantly lower minimum inhibitory concentration(4.47±0.01μl/ml)against the Staphylococcus aureus.In addition,LW-CQDs revealed a higher killing rate of reaction for S.aureus as compared to K.pneumoniae,P.aeruginosa,and S.typhi and remarkably degraded 92.95%of brilliant red dye under visible light(2000 lux).Hence,these facile LW-CQDs hold potential for applications in the effectiveness of antimicrobial and photocatalytic dye reduction activity,which show valuable contributions to both waste valorization and sustainable material development.