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Solvatochromic BODIPYs with Polarity-Independent Absorption for Functional Super-resolution Imaging of Live Cell Membranes 认领 引用
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作者 Yunshu Liu Xiayi Gong +4 位作者 Bryn Yoder Jessica M.Gluck Nelson R.Vinueza Xiaojing Liu Yang Zhang 《Chemical & Biomedical Imaging》 CAS CSCD 2026年第4期527-532,共6页
Functional super-resolution microscopy(SRM)has recently emerged as a powerful technique for concurrently mapping nanoscale physicochemical properties of the subcellular environment.This is accomplished by capturing bo... Functional super-resolution microscopy(SRM)has recently emerged as a powerful technique for concurrently mapping nanoscale physicochemical properties of the subcellular environment.This is accomplished by capturing both the spatial localization and single-molecule fluorescence spectral shifts of environmentally sensitive fluorescent probes.However,current fluorophore choices for functional SRM are largely limited to Nile Red derivatives,which suffer from several limitations.Here we report two solvatochromic and far-red-emitting fluorophores with polarityindependent absorption specifically tailored for functional SRM,based on a boron dipyrromethene(BODIPY)chromophore.We systematically characterize their photophysical and solvatochromic properties and directly compare their performance to that of Nile Red.A zwitterionic lipid is introduced to the dye to enable transient-binding-based single-molecule switching and to achieve selective targeting of the plasma membrane in live cells.The functional SRM capability of this probe is demonstrated by mapping nanoscale membrane polarity changes under the influence of cholesterol in live U2OS cells with a localization precision of 16−20 nm. 展开更多
关键词 BODIPY Solvatochromism Functional Super-resolution Microscopy Single-Molecule Spectroscopy Polarity Mapping Membrane Imaging
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