Theoretical analysis and numerical simulations using computational fluid dynamics(CFD)were conducted to investigate hypersonic laminar flow over a compression corner,focusing on the effects of arbitrary surface cataly...Theoretical analysis and numerical simulations using computational fluid dynamics(CFD)were conducted to investigate hypersonic laminar flow over a compression corner,focusing on the effects of arbitrary surface catalysis on peak surface heat transfer.Four groups of inflow conditions were considered,varying in Mach number(8-12),unit Reynolds number(2×105-8×105m-1),degree of dissociation(0.05-0.15),and ramp angle(20°-28°).The results indicate that while wall catalysis has a negligible effect on the flow structure,it significantly influences the peak surface heat transfer near the reattachment region,even for finite-rate catalytic walls.A predictive formula is proposed for the non-dimensional catalytic heating,considering finite-rate catalytic walls.CFD results show that the peak heat flux increases as the catalytic coefficient increases due to enhanced surface recombination of atoms,and the effectiveness of the formula is further verified by oxygen inflow.Finally,the catalytic heating ratio at the location of peak surface heat transfer is evaluated by the formula using catalytic coefficients of real materials.It is shown that the catalytic heat flux ratio may increase by approximately 50%from oxygen inflow to nitrogen inflow for copper under the same nominal freestream.展开更多
X-ray detectors,as crucial elements in medical imaging and industrial fields,can be categorized into direct and indirect types.Direct detectors,which directly convert X-ray photons into electrical signals,exhibit high...X-ray detectors,as crucial elements in medical imaging and industrial fields,can be categorized into direct and indirect types.Direct detectors,which directly convert X-ray photons into electrical signals,exhibit high sensitivity and low detection limits,enabling the capture of high-resolution images and reducing radiation exposure to patients.Organic copper halides,recognized as potential active materials for X-ray detection,have been widely explored in the indirect scintillation field but remain under-explored in direct X-ray detector applications.In this work,(C12H12N)3Cu3I6is demonstrated as an efficient semiconductor for direct X-ray detection with excellent stability.A lateral-structured X-ray detector was fabricated with gold electrodes,which exhibits a maximum sensitivity of 1464.14μC·Gy-1·cm-2,a lowest detection limit of 19.8 nGy·s-1,a high on-off ratio of 2140,and an excellent operational stability of retaining 96%performance after 600 s continuous X-ray radiation.Furthermore,the detector successfully imaged a 0.1 mm“F”-shaped lead sheet,validating its capacity for X-ray imaging.This study highlights the potential of(C12H12N)3Cu3I6as a promising semiconductor for high-performance direct X-ray detection,expanding the application scope of organic copper halides in this critical field.展开更多
Orthogonal frequency division multiplexing (OFDM) waveform enables radar and communication functions simultaneously, which encounters low angle resolution and poor data rate for traditional single input single output ...Orthogonal frequency division multiplexing (OFDM) waveform enables radar and communication functions simultaneously, which encounters low angle resolution and poor data rate for traditional single input single output (SISO) systems. To solve these problems, an integrated radar and communication system (IRCS) with multiple input multiple output (MIMO) OFDM waveform is proposed. The different limitations of radar and communication in designing such a system are investigated. Then, an optimization problem is devised to obtain suitable system parameters, including the number of subcarriers, subcarrier spacing, number of symbols, pulse repetition frequency (PRF) and length of cyclic prefix (CP). Finally, to satisfy the requirements of both radar and communication, the IRCS parameters are derived in three typical cases. Several numerical results are presented to illustrate the demands of radar and communication, inconsistent or consistent, for the IRCS parameters and the superiority of the proposed system.展开更多
The transmit antenna beampattern of the phased array radar is only a function of angle,limiting its ability to discriminate the targets from the same direction.Recently,the waveform diverse array radars expand the ang...The transmit antenna beampattern of the phased array radar is only a function of angle,limiting its ability to discriminate the targets from the same direction.Recently,the waveform diverse array radars expand the angle-dependent beampattern to an angle-time-range-dependent three-dimensional function by modulating the frequenciesime delays/phases across different transmit antenna elements.In this respect,extra Degrees-of-Freedom(DOFs)in the range domain are achieved,which opens up an innovative way to fulfil the tasks with enhanced system performance by jointly using the angle and range information.This paper summaries the developments of waveform diverse radars,including the Frequency Diverse Array(FDA),the Space-Time-CirculatingArray(STCA),and the Element-Pulse-Coding(EPC)frameworks,with emphasis on the analysis of the range-dependent beampattern from the basic properties upon how it is controlled.Moreover,the most recent advances of utilizing such a range-dependent beampattern in target detection,parameter estimation and identifiability,clutter suppression,jammer suppression and Synthetic Aperture Radar(SAR)imaging are discussed.展开更多
New pollutant pharmaceutical and personal care products(PPCPs),especially antiviral drugs,have received increasing attention not only due to their increase in usage after the outbreak of COVID-19 epidemics but also du...New pollutant pharmaceutical and personal care products(PPCPs),especially antiviral drugs,have received increasing attention not only due to their increase in usage after the outbreak of COVID-19 epidemics but also due to their adverse impacts on water ecological environment.Electro-Fenton technology is an effective method to remove PPCPs from water.Novel particle electrodes(MMTGO/Fe3O4)were synthesized by depositing Fe3O4 nanoparticles on reduced graphene oxide modified montmorillonite and acted as catalysts to promote oxidation performance in a three-dimensional electro-Fenton(3D-EF)system.The electrodes combined the catalytic property of Fe3O4,hydrophilicity of montmorillonite and electrical conductivity of graphene oxides,and applied for the degradation of Acyclovir(ACV)with high efficiency and ease of operation.At optimal condition,the degradation rate of ACV reached 100%within 120 min,and the applicable pH range could be 3 to 11 in the 3D-EF system.The stability and reusability of MMTGO/Fe3O4particle electrodes were also studied,the removal rate of ACV remained at 92%after 10 cycles,which was just slightly lower than that of the first cycle.Potential degradation mechanisms were also proposed by methanol quenching tests and FT-ICR-MS.展开更多
Frequency diverse array(FDA)radar has been studied for more than 15 years and has attracted a lot of attention due to its potential advantages over the well-known phased array radar.The representative feature of FDA i...Frequency diverse array(FDA)radar has been studied for more than 15 years and has attracted a lot of attention due to its potential advantages over the well-known phased array radar.The representative feature of FDA is range-angle-time-dependent transmit beampattern and its underlying properties are continuously revealed in the research.The formulation and exploitation of the transmit diversity with a frequency increment is the fundamental principle,which brings extra degrees-of-freedom(DOFs)in the transmit dimension.As the FDA radar carries additional information in range,it provides more flexibility in signal processing and also brings in new technical issues.This article overviews the state-of-the-art in FDA radar area and its applications,mainly based on the progress in our group.There are two main catalogs in FDA radar area,namely coherent FDA and FDA-MIMO(multiple-input multiple-output)radars.Potential applications including target parameter estimation,ambiguous clutter suppression,and deceptive jammer suppression are discussed.展开更多
The role of macrophages (MФ) as the first line of host defense is well accepted. These cells play a central role in orchestrating crucial functions during schistosomal infection. Thus, understanding the functional ...The role of macrophages (MФ) as the first line of host defense is well accepted. These cells play a central role in orchestrating crucial functions during schistosomal infection. Thus, understanding the functional diversity of these cells in the process of infection as well as the mechanisms underlying these events is crucial for developing disease control strategies. In this study, we adopted a Mqb polarization recognition system. M1 macrophage was characterized by expressing CD16/32, IL-12 and iNOS. M2 macrophage was characterized by expressing CD206, IL-10 and arg-1. In vivo (mouse peritoneal macrophages of different infection stages were obtained) and in vitro (different S. japonicum antigens were used to stimulate RAW264.7) were characterized by using the above mentioned system. NCA and ACA stimulated RAW264.7 express significantly higher levels of IL-12 while significantly higher levels of IL-10 were detected after soluble egg antigen (SEA) stimulation. The results showed that dramatic changes of antigen in the microenvironment before and after egg production led to macrophage polarization. Furthermore, through TLR blocking experiments, the TLR4 signaling pathway was found to play a role in the process of macrophage polarization toward M1. Our data suggest that macrophage polarization during S. japonicum infection had significant effects on host immune responses to S. japonicum.展开更多
Multimodal imaging-guided chemo-photothermal therapy is an excellent cancer treatment,which can not only efficiently against tumor,but also can offer precise treatment window and real-time monitoring of the treatment ...Multimodal imaging-guided chemo-photothermal therapy is an excellent cancer treatment,which can not only efficiently against tumor,but also can offer precise treatment window and real-time monitoring of the treatment efficiency.In our work,polydopamine(PDA)-coated gold nanobones(AuNBs@PDA nanocomplexes)were designed for this approach.The AuNBs@PDA nanocomplexes have strong absorbance in the near infrared(NIR)region and higher photothermal conversion efficiency(75.48%)than gold nanobones alone,which was facilitated for photoacoustic imaging and photothermal therapy.Besides,the loading efficiency of doxorubicin(DOX)by AuNBs@PDA nanocomplexes could be up to about 70%and DOX release from AuNBs@PDA/DOX nanocomplexes sensitively response to the lower pH environment and NIR laser irradiation,which makes them become the excellent nano-carrier for the delivery of chemotherapy drug.In vitro and in vivo studies showed significant cytotoxicity and antitumor efficacy by the AuNBs@PDA/DOX nanoplatform with negligible side effects.Meanwhile,the nanoplatform was also successfully employed for computed tomography(CT)imaging,attributing to the high atomic number and high X-ray attenuation coefficient of gold.Therefore,we believed that the proposed PDA-coated gold nanobones would be a novel multifunctional theranostic nanoagent to realize the PA/CT imaging-guided chemo-photothermal therapy of cancer.展开更多
This study was conducted to investigate the effects of extracts of 4 Tibetan veterinary drugs on bacterial diarrhea, and to guide the clinical treatment of the disease. The inhibitory effects of the extracts of the 4 ...This study was conducted to investigate the effects of extracts of 4 Tibetan veterinary drugs on bacterial diarrhea, and to guide the clinical treatment of the disease. The inhibitory effects of the extracts of the 4 Tibetan veterinary drugs, Veronica ciliate Fisch, Usnea diffracta Vain, Sophoraflavescens var. flavescens, Lamiophlomis rotata ( Benth. ) Kudo on 14 common diarrheagenic bacteria were detected by K-B diffusion method and micro-broth dilution method. Optimization was performed on prescriptions of the 4 Tibetan veterinary drugs using orthogonal design software, and a mouse bacterial diarrhea model was established with a clinical isolate, Salmonella blegdam. According to the LD50 value of the optimal prescription of the 4 Tibetan veterinary drugs, the established mouse bacterial diarrhea model was treated in 3 dose groups, i. e. , the high, middle and low dose groups (0. 060, 0. 030 and 0. 020 g/ml, respectively). The results showed that the 4 Tibetan veterinary drugs had very good inhibitory effects on most of the tested diarrheagenic bacteria, and among them, U. diffracta had better inhibitory effects on all the tested bacteria. The optimal prescription of the d Tibetan veterinary drugs (high dose, 0.06 g/ml) exhibited very good inhibitory effect on mouse diarrhea, indicating that the prescription has very good anti-diarrhea effect, which is beneficial to the clinical treatment of such disease and the development of Tibetan veterinary drugs.展开更多
Nowadays,location-based services are widely used,requiring instant responses to a large volume of multiple spatial queries over massive road networks,i.e.,single-pair shortest path(SPSP)query,k-nearest neighbor(kNN)qu...Nowadays,location-based services are widely used,requiring instant responses to a large volume of multiple spatial queries over massive road networks,i.e.,single-pair shortest path(SPSP)query,k-nearest neighbor(kNN)query,and range query.Creating index-based structure for each kind of query is costly,hence it is important to handle multiple spatial queries within one efficient structure.Partition-based hierarchical approaches show promising potential to meet the requirement.However,existing approaches require large search space on massive road networks especially for long-distance queries,which is inefficient and hard to scale.To overcome the drawbacks,we propose the shortcut-enhanced graph hierarchy tree(SCG-tree),which leverages shortcuts to effectively prune the search space over a hierarchical structure.With the SCG-tree,a pruned shortcut-based method is designed to answer SPSP query,and a two-phase expansion strategy is proposed to leverage shortcuts for kNN and range queries.Theoretical analyses show the superiority of proposed shortcut-based query algorithms.Extensive experiments demonstrate that our approach can achieve three times speedup for kNN query and an order of magnitude speedup for SPSP and range queries over existing methods on real road networks that scale up to 24 million nodes and 58 million edges.展开更多
Posterior capsule opacification(PCO)is the most common long-term postoperative complication of cataract surgery,leading to secondary vision loss.Optimized intraocular lens(IOL)structure and appropriate pharmacological...Posterior capsule opacification(PCO)is the most common long-term postoperative complication of cataract surgery,leading to secondary vision loss.Optimized intraocular lens(IOL)structure and appropriate pharmacological intervention,which provides physical barriers and biological inhibition,respectively,can block the migration,proliferation,and epithelial-mesenchymal transition(EMT)of lens epithelial cells(LECs)for PCO prophylaxis.Herein,a novel indomethacin-eluting IOL(INDOM-IOL)with an optimized sharper edge and a sustained drug release behavior was developed for PCO prevention.Indomethacin(INDOM),an ophthalmic non-steroidal anti-inflammatory drug(NSAID)used for postoperative ocular inflammation,was demonstrated to not only be able to suppress cell migration and down-regulate the expression of cyclooxygenase-2(COX-2)and EMT markers,including alpha-smooth muscle actin(α-SMA)and cyclin D1,but also promote the autophagy activation in LECs.Additionally,autophagy was also verified to be a potential therapeutic target for the down-regulation of EMT in LECs.The novel IOL,serving as a drug delivery platform,could carry an adjustable dose of hydrophobic indomethacin with sustained drug release ability for more than 28 days.In the rabbit PCO model,the indomethacin-eluting IOL showed excellent anti-inflammatory and anti-PCO effects.In summary,indomethacin is an effective pharmacological intervention in PCO prophylaxis,and the novel IOL we developed prevented PCO in vivo under its sustained indomethacin release property,which provided a promising approach for PCO prophylaxis in clinical application.展开更多
Hyaluronic acid(HA)-based hydrogels have applied widely for biomedical applications due to its biocompatibility and biodegradability.However,the use of initiators or crosslinkers during the hydrogel formation may caus...Hyaluronic acid(HA)-based hydrogels have applied widely for biomedical applications due to its biocompatibility and biodegradability.However,the use of initiators or crosslinkers during the hydrogel formation may cause cytotoxicity and thereby impair the biocompatibility.Inspired by the crosslinking mechanism of fibrin gel,a novel HA-based hydrogel was developed via the in situ supramolecular assembly based on knob-hole interactions between fibrinogen and knob-grafted HA(knob-g-HA)in this study.The knob-grafted HA was synthesized by coupling knob peptides(GPRPAAC,a mimic peptide of fibrin knob A)to HA via Michael addition.Then the translucent fibrinogen/knob-g-HA hydrogels were prepared by simply mixing the solutions of knob-g-HA and fibrinogen at the knob/hole ratio of 1.2.The rheological behaviors of the fibrinogen/knob-g-HA hydrogels with the fibrinogen concentrations of 50,100 and 200 mg/mL were evaluated,and it was found that the dynamic storage moduli(G0)were higher than the loss moduli(G00)over the whole frequency range for all the groups.The SEM results showed that fibrinogen/knob-g-HA hydrogels presented the heterogeneous mesh-like structures which were different from the honeycomb-like structures of fibrinogen/MA-HA hydrogels.Correspondingly,a higher swelling ratio was obtained in the groups of fibrinogen/knob-g-HA hydrogel.Finally,the cytocompatibility of fibrinogen/knob-g-HA hydrogels was proved by live/dead stainings and MTT assays in the 293T cells encapsulation test.All these results highlight the biological potential of the fibrinogen/knob-g-HA hydrogels for 3D cellular engineering.展开更多
Working principles of silicon double P-N junction color sensor are introduced and a color measurement system to distinguish tooth color difference is designed in this paper.This system consists of silicon double P-N j...Working principles of silicon double P-N junction color sensor are introduced and a color measurement system to distinguish tooth color difference is designed in this paper.This system consists of silicon double P-N junction,a small optical fiber probe,signal process circuit and an MSP430FG439 single-chip system.Small in size,this system can measure different parts of the tooth in a fast and convenient way with high-accuracy.Thus,this system will be very promising in building prosthodontics and tooth fabrication.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.12372296)the Strategic Priority Research Program B of the Chinese Academy of Sciences(Grant No.XDB0620203)。
摘要Theoretical analysis and numerical simulations using computational fluid dynamics(CFD)were conducted to investigate hypersonic laminar flow over a compression corner,focusing on the effects of arbitrary surface catalysis on peak surface heat transfer.Four groups of inflow conditions were considered,varying in Mach number(8-12),unit Reynolds number(2×105-8×105m-1),degree of dissociation(0.05-0.15),and ramp angle(20°-28°).The results indicate that while wall catalysis has a negligible effect on the flow structure,it significantly influences the peak surface heat transfer near the reattachment region,even for finite-rate catalytic walls.A predictive formula is proposed for the non-dimensional catalytic heating,considering finite-rate catalytic walls.CFD results show that the peak heat flux increases as the catalytic coefficient increases due to enhanced surface recombination of atoms,and the effectiveness of the formula is further verified by oxygen inflow.Finally,the catalytic heating ratio at the location of peak surface heat transfer is evaluated by the formula using catalytic coefficients of real materials.It is shown that the catalytic heat flux ratio may increase by approximately 50%from oxygen inflow to nitrogen inflow for copper under the same nominal freestream.
基金supported by the National Natural Science Foundation of China(62305195)the Shandong Provincial Natural Science Foundation(ZR2022QF036 and ZR2025MS1001)Shandong Provincial Youth Innovation Technology Program(2024KJN010).
摘要X-ray detectors,as crucial elements in medical imaging and industrial fields,can be categorized into direct and indirect types.Direct detectors,which directly convert X-ray photons into electrical signals,exhibit high sensitivity and low detection limits,enabling the capture of high-resolution images and reducing radiation exposure to patients.Organic copper halides,recognized as potential active materials for X-ray detection,have been widely explored in the indirect scintillation field but remain under-explored in direct X-ray detector applications.In this work,(C12H12N)3Cu3I6is demonstrated as an efficient semiconductor for direct X-ray detection with excellent stability.A lateral-structured X-ray detector was fabricated with gold electrodes,which exhibits a maximum sensitivity of 1464.14μC·Gy-1·cm-2,a lowest detection limit of 19.8 nGy·s-1,a high on-off ratio of 2140,and an excellent operational stability of retaining 96%performance after 600 s continuous X-ray radiation.Furthermore,the detector successfully imaged a 0.1 mm“F”-shaped lead sheet,validating its capacity for X-ray imaging.This study highlights the potential of(C12H12N)3Cu3I6as a promising semiconductor for high-performance direct X-ray detection,expanding the application scope of organic copper halides in this critical field.
基金supported by the National Natural Science Foundation of China(6123101761671352)
摘要Orthogonal frequency division multiplexing (OFDM) waveform enables radar and communication functions simultaneously, which encounters low angle resolution and poor data rate for traditional single input single output (SISO) systems. To solve these problems, an integrated radar and communication system (IRCS) with multiple input multiple output (MIMO) OFDM waveform is proposed. The different limitations of radar and communication in designing such a system are investigated. Then, an optimization problem is devised to obtain suitable system parameters, including the number of subcarriers, subcarrier spacing, number of symbols, pulse repetition frequency (PRF) and length of cyclic prefix (CP). Finally, to satisfy the requirements of both radar and communication, the IRCS parameters are derived in three typical cases. Several numerical results are presented to illustrate the demands of radar and communication, inconsistent or consistent, for the IRCS parameters and the superiority of the proposed system.
基金supported in part by the National Nature Science Foundation of China(Nos.62101402,61931016,62071344)China Postdoctoral Science Foundation(Nos.2021TQ0261,2021M702547)Young Elite Scientists Sponsorship Program by CAST(2021QNRC001).
摘要The transmit antenna beampattern of the phased array radar is only a function of angle,limiting its ability to discriminate the targets from the same direction.Recently,the waveform diverse array radars expand the angle-dependent beampattern to an angle-time-range-dependent three-dimensional function by modulating the frequenciesime delays/phases across different transmit antenna elements.In this respect,extra Degrees-of-Freedom(DOFs)in the range domain are achieved,which opens up an innovative way to fulfil the tasks with enhanced system performance by jointly using the angle and range information.This paper summaries the developments of waveform diverse radars,including the Frequency Diverse Array(FDA),the Space-Time-CirculatingArray(STCA),and the Element-Pulse-Coding(EPC)frameworks,with emphasis on the analysis of the range-dependent beampattern from the basic properties upon how it is controlled.Moreover,the most recent advances of utilizing such a range-dependent beampattern in target detection,parameter estimation and identifiability,clutter suppression,jammer suppression and Synthetic Aperture Radar(SAR)imaging are discussed.
基金the GDAS’Project of Science and Technology Development(No.2020GDASYL-20200103044)Key-Area Research and Development Program of Guangdong(No.2020B1111350002)+1 种基金the National Key R&D Program of China(No.2019YFC1805305)the Project of Water Resource Department of Guangdong Province(No.2017-18).
摘要New pollutant pharmaceutical and personal care products(PPCPs),especially antiviral drugs,have received increasing attention not only due to their increase in usage after the outbreak of COVID-19 epidemics but also due to their adverse impacts on water ecological environment.Electro-Fenton technology is an effective method to remove PPCPs from water.Novel particle electrodes(MMTGO/Fe3O4)were synthesized by depositing Fe3O4 nanoparticles on reduced graphene oxide modified montmorillonite and acted as catalysts to promote oxidation performance in a three-dimensional electro-Fenton(3D-EF)system.The electrodes combined the catalytic property of Fe3O4,hydrophilicity of montmorillonite and electrical conductivity of graphene oxides,and applied for the degradation of Acyclovir(ACV)with high efficiency and ease of operation.At optimal condition,the degradation rate of ACV reached 100%within 120 min,and the applicable pH range could be 3 to 11 in the 3D-EF system.The stability and reusability of MMTGO/Fe3O4particle electrodes were also studied,the removal rate of ACV remained at 92%after 10 cycles,which was just slightly lower than that of the first cycle.Potential degradation mechanisms were also proposed by methanol quenching tests and FT-ICR-MS.
基金This work was supported in part by the National Natural Science Foundation of China(NSFC)(Nos.61931016,62071344 and 61911530246)the Key Laboratory Equipment Advanced Research Fund(No.6142206200210).
摘要Frequency diverse array(FDA)radar has been studied for more than 15 years and has attracted a lot of attention due to its potential advantages over the well-known phased array radar.The representative feature of FDA is range-angle-time-dependent transmit beampattern and its underlying properties are continuously revealed in the research.The formulation and exploitation of the transmit diversity with a frequency increment is the fundamental principle,which brings extra degrees-of-freedom(DOFs)in the transmit dimension.As the FDA radar carries additional information in range,it provides more flexibility in signal processing and also brings in new technical issues.This article overviews the state-of-the-art in FDA radar area and its applications,mainly based on the progress in our group.There are two main catalogs in FDA radar area,namely coherent FDA and FDA-MIMO(multiple-input multiple-output)radars.Potential applications including target parameter estimation,ambiguous clutter suppression,and deceptive jammer suppression are discussed.
摘要The role of macrophages (MФ) as the first line of host defense is well accepted. These cells play a central role in orchestrating crucial functions during schistosomal infection. Thus, understanding the functional diversity of these cells in the process of infection as well as the mechanisms underlying these events is crucial for developing disease control strategies. In this study, we adopted a Mqb polarization recognition system. M1 macrophage was characterized by expressing CD16/32, IL-12 and iNOS. M2 macrophage was characterized by expressing CD206, IL-10 and arg-1. In vivo (mouse peritoneal macrophages of different infection stages were obtained) and in vitro (different S. japonicum antigens were used to stimulate RAW264.7) were characterized by using the above mentioned system. NCA and ACA stimulated RAW264.7 express significantly higher levels of IL-12 while significantly higher levels of IL-10 were detected after soluble egg antigen (SEA) stimulation. The results showed that dramatic changes of antigen in the microenvironment before and after egg production led to macrophage polarization. Furthermore, through TLR blocking experiments, the TLR4 signaling pathway was found to play a role in the process of macrophage polarization toward M1. Our data suggest that macrophage polarization during S. japonicum infection had significant effects on host immune responses to S. japonicum.
基金financially supported by the National Key R&D Program of China(No.2018YFB1105700)the National Natural Science Foundation of China(Nos.81902913,81930048 and 81627805)+9 种基金the Guangdong Basic and Applied Basic Research Foundation for Distinguished Young Scholars(No.2020B1515020027)the Fundamental Research Funds for the Central Universities(No.19ykpy108)the Natural Science Foundation of Jiangsu Province(No.BK20190821)the Postdoctoral Science Foundation of China(No.2019M651953)the Open Project Program of the State Key Laboratory of Radiation Medicine and Protection,Soochow University(No.GZK1201909)the Guangdong Science and Technology Department(No.2020B1212060018)the Science and Technology Project from Suzhou City Commission of Health and Family Planning(No.LCZX201712)the grants from Guangzhou Science and Technology Bureau(202002020070,201902020015)Opening Foundation of Hubei Province Key Laboratory of Molecular Imaginga Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions。
摘要Multimodal imaging-guided chemo-photothermal therapy is an excellent cancer treatment,which can not only efficiently against tumor,but also can offer precise treatment window and real-time monitoring of the treatment efficiency.In our work,polydopamine(PDA)-coated gold nanobones(AuNBs@PDA nanocomplexes)were designed for this approach.The AuNBs@PDA nanocomplexes have strong absorbance in the near infrared(NIR)region and higher photothermal conversion efficiency(75.48%)than gold nanobones alone,which was facilitated for photoacoustic imaging and photothermal therapy.Besides,the loading efficiency of doxorubicin(DOX)by AuNBs@PDA nanocomplexes could be up to about 70%and DOX release from AuNBs@PDA/DOX nanocomplexes sensitively response to the lower pH environment and NIR laser irradiation,which makes them become the excellent nano-carrier for the delivery of chemotherapy drug.In vitro and in vivo studies showed significant cytotoxicity and antitumor efficacy by the AuNBs@PDA/DOX nanoplatform with negligible side effects.Meanwhile,the nanoplatform was also successfully employed for computed tomography(CT)imaging,attributing to the high atomic number and high X-ray attenuation coefficient of gold.Therefore,we believed that the proposed PDA-coated gold nanobones would be a novel multifunctional theranostic nanoagent to realize the PA/CT imaging-guided chemo-photothermal therapy of cancer.
基金Supported by the Science and Technology Training Plan Project of Sichuan Province(2016KZ0007)the Fundamental Research Funds for the Central Universitiesof Southwest University for Nationalities(2017NZYQN38)
摘要This study was conducted to investigate the effects of extracts of 4 Tibetan veterinary drugs on bacterial diarrhea, and to guide the clinical treatment of the disease. The inhibitory effects of the extracts of the 4 Tibetan veterinary drugs, Veronica ciliate Fisch, Usnea diffracta Vain, Sophoraflavescens var. flavescens, Lamiophlomis rotata ( Benth. ) Kudo on 14 common diarrheagenic bacteria were detected by K-B diffusion method and micro-broth dilution method. Optimization was performed on prescriptions of the 4 Tibetan veterinary drugs using orthogonal design software, and a mouse bacterial diarrhea model was established with a clinical isolate, Salmonella blegdam. According to the LD50 value of the optimal prescription of the 4 Tibetan veterinary drugs, the established mouse bacterial diarrhea model was treated in 3 dose groups, i. e. , the high, middle and low dose groups (0. 060, 0. 030 and 0. 020 g/ml, respectively). The results showed that the 4 Tibetan veterinary drugs had very good inhibitory effects on most of the tested diarrheagenic bacteria, and among them, U. diffracta had better inhibitory effects on all the tested bacteria. The optimal prescription of the d Tibetan veterinary drugs (high dose, 0.06 g/ml) exhibited very good inhibitory effect on mouse diarrhea, indicating that the prescription has very good anti-diarrhea effect, which is beneficial to the clinical treatment of such disease and the development of Tibetan veterinary drugs.
基金supported by the Frontier Technology R&D Project of Jiangsu(BF2024059)the National Natural Science Foundation of China(Grant No.6240071854)+1 种基金the Natural Science Foundation of Jiangsu Province(BK20241381)the Jiangsu Association for Science and Technology Youth Science and Technology Talent Support Project(JSTJ-2023-XH055).
摘要Nowadays,location-based services are widely used,requiring instant responses to a large volume of multiple spatial queries over massive road networks,i.e.,single-pair shortest path(SPSP)query,k-nearest neighbor(kNN)query,and range query.Creating index-based structure for each kind of query is costly,hence it is important to handle multiple spatial queries within one efficient structure.Partition-based hierarchical approaches show promising potential to meet the requirement.However,existing approaches require large search space on massive road networks especially for long-distance queries,which is inefficient and hard to scale.To overcome the drawbacks,we propose the shortcut-enhanced graph hierarchy tree(SCG-tree),which leverages shortcuts to effectively prune the search space over a hierarchical structure.With the SCG-tree,a pruned shortcut-based method is designed to answer SPSP query,and a two-phase expansion strategy is proposed to leverage shortcuts for kNN and range queries.Theoretical analyses show the superiority of proposed shortcut-based query algorithms.Extensive experiments demonstrate that our approach can achieve three times speedup for kNN query and an order of magnitude speedup for SPSP and range queries over existing methods on real road networks that scale up to 24 million nodes and 58 million edges.
基金This work was supported by the National Key Research and Development Program of China(Grant numbers 2020YFE0204400)National Natural Science Foundation of China(Grant numbers 82271063,82271064,52203190,82070939,and 22005265)+2 种基金Key Research and Development Project of Zhejiang Province(Grant number 2020C03035)Postdoctoral Science Foundation of China(Grant number 2020TQ0261)Fundamental Research Funds for the Central Universities(Grant number 2021FZZX003-01-03).
摘要Posterior capsule opacification(PCO)is the most common long-term postoperative complication of cataract surgery,leading to secondary vision loss.Optimized intraocular lens(IOL)structure and appropriate pharmacological intervention,which provides physical barriers and biological inhibition,respectively,can block the migration,proliferation,and epithelial-mesenchymal transition(EMT)of lens epithelial cells(LECs)for PCO prophylaxis.Herein,a novel indomethacin-eluting IOL(INDOM-IOL)with an optimized sharper edge and a sustained drug release behavior was developed for PCO prevention.Indomethacin(INDOM),an ophthalmic non-steroidal anti-inflammatory drug(NSAID)used for postoperative ocular inflammation,was demonstrated to not only be able to suppress cell migration and down-regulate the expression of cyclooxygenase-2(COX-2)and EMT markers,including alpha-smooth muscle actin(α-SMA)and cyclin D1,but also promote the autophagy activation in LECs.Additionally,autophagy was also verified to be a potential therapeutic target for the down-regulation of EMT in LECs.The novel IOL,serving as a drug delivery platform,could carry an adjustable dose of hydrophobic indomethacin with sustained drug release ability for more than 28 days.In the rabbit PCO model,the indomethacin-eluting IOL showed excellent anti-inflammatory and anti-PCO effects.In summary,indomethacin is an effective pharmacological intervention in PCO prophylaxis,and the novel IOL we developed prevented PCO in vivo under its sustained indomethacin release property,which provided a promising approach for PCO prophylaxis in clinical application.
基金The authors acknowledge the financial support by the National Natural Science Foundation of China(51673167 and 21434006)the National Key Research Programof China(2017YFA0104901)the Fundamental Research Funds for the Central Universities(2017XZZX008-05).
摘要Hyaluronic acid(HA)-based hydrogels have applied widely for biomedical applications due to its biocompatibility and biodegradability.However,the use of initiators or crosslinkers during the hydrogel formation may cause cytotoxicity and thereby impair the biocompatibility.Inspired by the crosslinking mechanism of fibrin gel,a novel HA-based hydrogel was developed via the in situ supramolecular assembly based on knob-hole interactions between fibrinogen and knob-grafted HA(knob-g-HA)in this study.The knob-grafted HA was synthesized by coupling knob peptides(GPRPAAC,a mimic peptide of fibrin knob A)to HA via Michael addition.Then the translucent fibrinogen/knob-g-HA hydrogels were prepared by simply mixing the solutions of knob-g-HA and fibrinogen at the knob/hole ratio of 1.2.The rheological behaviors of the fibrinogen/knob-g-HA hydrogels with the fibrinogen concentrations of 50,100 and 200 mg/mL were evaluated,and it was found that the dynamic storage moduli(G0)were higher than the loss moduli(G00)over the whole frequency range for all the groups.The SEM results showed that fibrinogen/knob-g-HA hydrogels presented the heterogeneous mesh-like structures which were different from the honeycomb-like structures of fibrinogen/MA-HA hydrogels.Correspondingly,a higher swelling ratio was obtained in the groups of fibrinogen/knob-g-HA hydrogel.Finally,the cytocompatibility of fibrinogen/knob-g-HA hydrogels was proved by live/dead stainings and MTT assays in the 293T cells encapsulation test.All these results highlight the biological potential of the fibrinogen/knob-g-HA hydrogels for 3D cellular engineering.
基金supported by the Innovation Fund for Small Technology-based Firms of China (No.06C26222200838).
摘要Working principles of silicon double P-N junction color sensor are introduced and a color measurement system to distinguish tooth color difference is designed in this paper.This system consists of silicon double P-N junction,a small optical fiber probe,signal process circuit and an MSP430FG439 single-chip system.Small in size,this system can measure different parts of the tooth in a fast and convenient way with high-accuracy.Thus,this system will be very promising in building prosthodontics and tooth fabrication.