期刊文献+
任意字段
题名或关键词
题名
关键词
摘要
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息
共找到2篇文章
< 1 >
每页显示 20 50 100
已选0条
引用分析
参考文献
引证文献
 统计分析
检索结果
已选文献
显示方式：
Recombinant Escherichia coli-driven whole-cell bioconversion for selective 5-Aminopentanol production as a novel bioplastic monomer 认领 引用
1
作者 Byung Wook Lee Hee Taek Kim +8 位作者 Hyun Gi Koh Kyungjae Yu Gaeul Kim Yoon Jung Jung Haeng-Geun Cha Yunhee Jeong Yung-Hun Yang See-Hyoung Park Kyungmoon Park 《Bioresources and Bioprocessing》 SCIE EI CSCD 2025年第1期944-954,共11页
5-Aminopentanol(5-AP)is a valuable amino alcohol with potential applications in polymer synthesis and bioplastics.Conventional production methods rely on petroleum-based feedstocks and metal catalysts,which raise envi... 5-Aminopentanol(5-AP)is a valuable amino alcohol with potential applications in polymer synthesis and bioplastics.Conventional production methods rely on petroleum-based feedstocks and metal catalysts,which raise environmental and sustainability concerns.In this study,a de novo biosynthetic pathway for 5-AP production from l-lysine was developed in Escherichia coli.The engineered pathway consisted of lysine decarboxylase 2(LdcC),putrescine aminotransferase(PatA),and tested aldehyde reductase(YahK,YihU,YqhD).Among the tested reductases,aldehyde reductase exhibited the highest catalytic efficiency,producing 44.5±2.6 mM of 5-AP(0.44±0.03 mol5−AP/molL−lysine).The replacement of the expression system with a T7-based dual-plasmid platform,pET24ma::ldcC,and pCDFDuet-1::yqhD::patA co-transformed into E.coli,increased the production to 60.7±5.8 mM,accompanied by reduced cadaverine accumulation.Further enhancement was achieved by increasing the gene dosage of PatA,leading to 68.5±4.2 mM 5-AP and reduced by 40%in cadaverine levels.Cadaverine is a precursor in the production of 5-AP,and its accumulation is an important factor in the limitation of conversion to 5-AP.Intracellular cofactor regeneration is expected to cause an indirect supply ofα-KG,a cofactor,to enhance conversion to 5-AP.To support intracellular cofactor regeneration,glucose supplementation and increased aeration were applied,resulting in a final titer of 78.5±1.2 mM 5-AP and improved precursor utilization.This study is the first report of selective microbial 5-AP production and highlights the importance of PatA expression in pathway optimization.The newly established l-lysine(C6)valorization process which converts l-lysine to high-value materials such as 1,5-PDO,glutarate,and 5-AP offers a promising route for the sustainable biosynthesis of amino alcohols,laying the groundwork for future improvements through enzyme engineering and metabolic design. 展开更多
关键词 5-Aminopentanol L-Lysine(C6)valorization process Whole-cell bioconversion Escherichia coli Novel bioplastic monomer
暂未订购 下载PDF
Interaction Energies of Tetraalkylphosphonium Cation- and Amino Acid Anion- Based Ionic Liquids 认领 引用
2
作者 YoonKook Park Kyungmoon Park 《Journal of Chemistry and Chemical Engineering》 2010年第9期54-60,共7页
Ab initio calculations were performed using the Gaussian 03 software package to obtain the optimized structure,interaction energy and infrared spectrum of ionic liquids(ILs)containing a tetraalkylphosphonium cation an... Ab initio calculations were performed using the Gaussian 03 software package to obtain the optimized structure,interaction energy and infrared spectrum of ionic liquids(ILs)containing a tetraalkylphosphonium cation and an amino acid anion.In order to investigate the effect of the presence of nitrogen in the cation,the tetraalkylphosphonium cation was replaced by a tetraalkylammonium cation,while amino acids were retained as anions in the ionic liquid.The amino acids studied here included glycine,alanine,serine,lysine,and glutamine.Optimized structures of several ILs in the gas phase were determined at the levels of Hartree-Fock(HF/6-3+G(d))and 1density functional theory(B3LYP/6-31++G(d,p)).At high-level estimations,MP2/6-31+G(d)and MP2/6-31++G(d,p),the interaction energies between the cations and anions of the ILs were determined.In order to investigate the effect of alkyl-chain length on interaction energy,the alkyl groups were varied from methyl to ethyl and propyl in the tetraalkylphosphonium functional groups.This procedure revealed that there is a considerably stronger interaction between the alkylphosphonium cations and amino acids than between alkylammonium cations and amino acids.Vibrational spectroscopy of ILs shows that a significant-OH vibration peak occurs at 4124 cmt in serine-containing ILs,and a prominent C=O vending peak occurs at 1626 cm^-1 in amino acids. 展开更多
关键词 Tetraalkylphosphonium tetraalkylammonium ab initio calculation interaction energy amino acid-based ionic liquid.
暂未订购 下载PDF
已选0条
引用分析
参考文献
引证文献
 统计分析
检索结果
已选文献
上一页 1 下一页 到第 页
在线咨询 使用帮助 返回顶部 意见反馈