This study focuses on achieving structural safety, cost-effectiveness, and practicality in high-rise buildings, conducting an in-depth exploration of these evolving requirements while examining the practical applicati...This study focuses on achieving structural safety, cost-effectiveness, and practicality in high-rise buildings, conducting an in-depth exploration of these evolving requirements while examining the practical applications and development trends of innovative architectural structural systems. By synthesizing theoretical frameworks and engineering project experiences domestically and internationally, the research clearly demonstrates the significant advantages of novel structural systems in optimizing construction configurations and enhancing load-bearing capacity. Through comprehensive comparative analysis of existing structural designs, the study details improvements in seismic resistance, service life durability, and construction efficiency. The conclusions indicate that such structural systems substantially improve the overall load-bearing performance of high-rise buildings, strengthen structural stability and resistance capabilities, and offer substantial potential for energy conservation, reduced resource consumption, and accelerated construction timelines. The primary objective of this research is to introduce a groundbreaking approach to high-rise building structural design, advance progress in architectural engineering technology, and provide a solid theoretical foundation along with actionable recommendations for the widespread adoption of innovative structural systems.展开更多
With the acceleration of urbanization and the widespread use of high-rise buildings, the stability of building structures has become a critical focus in engineering design, while the performance of building materials ...With the acceleration of urbanization and the widespread use of high-rise buildings, the stability of building structures has become a critical focus in engineering design, while the performance of building materials serves as a decisive factor in structural safety and durability. This study begins by examining the properties of building structural materials, comprehensively investigating their impact on overall structural stability through analyses of mechanical characteristics, durability, and seismic performance. Through detailed examinations of how common materials respond under various loading conditions, the research establishes a theoretical framework that elucidates the intrinsic relationship between material physical properties and structural behavior under compression, bending, and seismic forces. High-performance materials exhibit superior stiffness and fatigue resistance, demonstrating exceptional effectiveness in enhancing building deformation resistance and mitigating structural aging. Optimal material combinations can significantly improve seismic and vibration reduction capabilities under extreme conditions, thereby elevating overall structural safety. This study not only highlights the pivotal role of material performance in building stability but also provides theoretical foundations for material selection, structural design, and safety assessment, offering substantial guidance for refining relevant codes and establishing robust building safety systems.展开更多
One of the physiological functions of cellular prion protein(PrP C)is believed to work as a cellular resistance to oxidative stress,in which the octarepeats region within PrP plays an important role.However,the detail...One of the physiological functions of cellular prion protein(PrP C)is believed to work as a cellular resistance to oxidative stress,in which the octarepeats region within PrP plays an important role.However,the detailed mechanism is less clear.In this study,the expressing plasmids of wild-type PrP(PrP-PG5)and various PrP mutants containing 0(PrP-PG0),9(PrP-PG9)and 12(PrP-PG12)octarepeats were generated and PrP proteins were expressed both in E.coli and in mammalian cells.Protein aggregation and formation of carbonyl groups were clearly seen in the recombinant PrPs expressed from E.coli after treatment of H2O2.MTT and trypan blue staining assays revealed that the cells expressing the mutated PrPs within octarepeats are less viable than the cells expressing wild-type PrP.Statistically significant high levels of intracellular free radicals and low levels of glutathione peroxidase were observed in the cells transfected with plasmids containing deleted or inserted octarepeats.Remarkably more productions of carbonyl groups were detected in the cells expressing PrPs with deleted and inserted octarepeats after exposing to H2O2.Furthermore,cells expressing wild-type PrP showed stronger resistant activity to the challenge of H2O2 at certain extent than the mutated PrPs and mock.These data provided the evidences that the octarepeats number within PrP is critical for maintaining its activity of antioxidation.Loss of its protective function against oxidative stress may be one of the possible pathways for the mutated PrPs to involve in the pathogenesis of familial Creutzfeldt-Jacob diseases.展开更多
摘要This study focuses on achieving structural safety, cost-effectiveness, and practicality in high-rise buildings, conducting an in-depth exploration of these evolving requirements while examining the practical applications and development trends of innovative architectural structural systems. By synthesizing theoretical frameworks and engineering project experiences domestically and internationally, the research clearly demonstrates the significant advantages of novel structural systems in optimizing construction configurations and enhancing load-bearing capacity. Through comprehensive comparative analysis of existing structural designs, the study details improvements in seismic resistance, service life durability, and construction efficiency. The conclusions indicate that such structural systems substantially improve the overall load-bearing performance of high-rise buildings, strengthen structural stability and resistance capabilities, and offer substantial potential for energy conservation, reduced resource consumption, and accelerated construction timelines. The primary objective of this research is to introduce a groundbreaking approach to high-rise building structural design, advance progress in architectural engineering technology, and provide a solid theoretical foundation along with actionable recommendations for the widespread adoption of innovative structural systems.
摘要With the acceleration of urbanization and the widespread use of high-rise buildings, the stability of building structures has become a critical focus in engineering design, while the performance of building materials serves as a decisive factor in structural safety and durability. This study begins by examining the properties of building structural materials, comprehensively investigating their impact on overall structural stability through analyses of mechanical characteristics, durability, and seismic performance. Through detailed examinations of how common materials respond under various loading conditions, the research establishes a theoretical framework that elucidates the intrinsic relationship between material physical properties and structural behavior under compression, bending, and seismic forces. High-performance materials exhibit superior stiffness and fatigue resistance, demonstrating exceptional effectiveness in enhancing building deformation resistance and mitigating structural aging. Optimal material combinations can significantly improve seismic and vibration reduction capabilities under extreme conditions, thereby elevating overall structural safety. This study not only highlights the pivotal role of material performance in building stability but also provides theoretical foundations for material selection, structural design, and safety assessment, offering substantial guidance for refining relevant codes and establishing robust building safety systems.
基金the National Science and Technology Task Force Project(Grant No.2006BAD06A13-2)National Basic Research Program(973 Program)of China(Grant No.2007CB310505)National Natural Science Foundation of China(Grant Nos.30571672,30500018 and 30771914)
摘要One of the physiological functions of cellular prion protein(PrP C)is believed to work as a cellular resistance to oxidative stress,in which the octarepeats region within PrP plays an important role.However,the detailed mechanism is less clear.In this study,the expressing plasmids of wild-type PrP(PrP-PG5)and various PrP mutants containing 0(PrP-PG0),9(PrP-PG9)and 12(PrP-PG12)octarepeats were generated and PrP proteins were expressed both in E.coli and in mammalian cells.Protein aggregation and formation of carbonyl groups were clearly seen in the recombinant PrPs expressed from E.coli after treatment of H2O2.MTT and trypan blue staining assays revealed that the cells expressing the mutated PrPs within octarepeats are less viable than the cells expressing wild-type PrP.Statistically significant high levels of intracellular free radicals and low levels of glutathione peroxidase were observed in the cells transfected with plasmids containing deleted or inserted octarepeats.Remarkably more productions of carbonyl groups were detected in the cells expressing PrPs with deleted and inserted octarepeats after exposing to H2O2.Furthermore,cells expressing wild-type PrP showed stronger resistant activity to the challenge of H2O2 at certain extent than the mutated PrPs and mock.These data provided the evidences that the octarepeats number within PrP is critical for maintaining its activity of antioxidation.Loss of its protective function against oxidative stress may be one of the possible pathways for the mutated PrPs to involve in the pathogenesis of familial Creutzfeldt-Jacob diseases.