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Genomic origin of Citrus reticulata“Unshiu” 认领 引用 被引量:1
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作者 Shengjun Liu Luoyun Wang +11 位作者 Xiang Zhang Lifang Sun Fuzhi Ke Yue Huang Lizhi Song Haiping Ye Jianguo Xu Yuantao Xu Xia Wang Xiuxin Deng Gaoping Liu Qiang Xu 《Horticulture Research》 SCIE CSCD 2025年第5期24-32,共9页
Satsuma mandarin(Citrus reticulata“Unshiu”)is a global cultivar with superior fruit characteristics and ranking among the top citrus cultivars in terms of production.It is also a key contributor to citrus breeding.H... Satsuma mandarin(Citrus reticulata“Unshiu”)is a global cultivar with superior fruit characteristics and ranking among the top citrus cultivars in terms of production.It is also a key contributor to citrus breeding.However,the lack of high-quality genome makes the origin of Satsuma mandarin has long been a matter of debate.Here,we assembled a gap-free,high-quality genome of Satsuma mandarin.Meanwhile,we collected and sequenced 15 indigenous citrus varieties in Zhejiang Province,12 Satsuma mandarins,21 citrus hybrids related to Satsuma mandarin,10 modern citrus varieties,and 7 other mandarins.Through high-resolution genome analysis,we inferred that Satsuma mandarin originated from a cross between C.reticulata“Ruju”×C.reticulata“Bendiguang”and proposed that Satsuma mandarin most probably originated in East area in Zhejiang Province of China,where the two parents-like cultivars are still found in a sympatric region to date.These results provide new insights into the origin model of Satsuma mandarin.The spread of mandarin is also discussed,which probably associated with the culture exchange and trade activities between Japan and China from Tang Dynasty and afterwards. 展开更多
关键词 satsuma mandarin citrus genomic analysis citrus h citrus reticulata satsuma mandarinmeanwhilewe satsuma mandarins origin
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Achieving exceptional strength and ductility combination in a heterostructured Mg-Y alloy with densely refined twins 认领 引用 被引量:5
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作者 Yuliang Yang Yuxin Liu +6 位作者 Shuang Jiang Ye Yuan Weiye Chen Lifang Sun Zhufeng He Xiaoli Zhao Nan Jia 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2024年第22期132-145,共14页
Metals and alloys with heterogeneous microstructures are an emerging class of materials that exhibit exceptional mechanical properties,owing to the novel scientific principle of hetero-deformation induced(HDI)strength... Metals and alloys with heterogeneous microstructures are an emerging class of materials that exhibit exceptional mechanical properties,owing to the novel scientific principle of hetero-deformation induced(HDI)strengthening and hardening.For magnesium alloys,due to their low recrystallization temperature,poor ductility at room temperature,limited cold workability,and the tendency to generate strong basal texture during deformation,it is difficult to obtain heterostructures without relying on precipitation of the second phases.Here,three heterostructured Mg-2.9Y(wt.%)materials with varying accumulative equivalent true strains,i.e.,5%-5 cycles,7.5%-5 cycles,and 10%-5 cycles materials were fabricated via applying five complete triaxial compression cycles to the bulk alloy.The 5%-5 cycles material with an accumulative equivalent true strain of 0.37 is featured with long twin lamellae embedded in coarse grains.When the accumulative true strain increases to 0.72,a heterogeneous structure composed of long and short twin lamellae is formed inside the 7.5%-5 cycles material.As the equivalent true strain further increases to 1.01,the 10%-5 cycles material exhibits a mixed structure with densely refined twin lamellae embedded in the coarse-grained matrix.The room-temperature uniaxial tensile tests show that the yield strength of the materials processed by triaxial cyclic compression(TCC)has been significantly improved compared to that at the initial state,whereas ductility was not significantly sacrificed without the subsequent heat treatment.The dense and refined twin lamellae that serve as hard domains in this material provide a high density of interfaces and impede dislocation motion effectively.This results in significant HDI strengthening and hardening.These findings provide new insight into the design of heterostructured hexagonal close-packed materials with both high strength and good ductility. 展开更多
关键词 Heterostructure Magnesium alloy Twin Hetero-deformation induced strengthening and hardening
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Development and validation of a UPLC–MS/MS assay for the determination of gemcitabine and its L-carnitine ester derivative in rat plasma and its application in oral pharmacokinetics 认领 引用 被引量:2
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作者 Gang Wang Dongyang Zhao +10 位作者 Hongxiang Chen Dawei Ding Longfa Kou Lifang Sun Chenxia Hao Xincong Li Kai Jia Qiming Kan Xiaohong Liu Zhonggui He Jin Sun 《Asian Journal of Pharmaceutical Sciences》 SCIE CAS 2017年第5期478-485,共8页
A simple and rapid UPLC–MS/MS method to simultaneously determine gemcitabine and its L-carnitine ester derivative(2’-deoxy-2’, 2’-difluoro-N-((4-amino-4-oxobutanoyl) oxy)-4-(trimethyl amm-onio) butanoate-cytidine,... A simple and rapid UPLC–MS/MS method to simultaneously determine gemcitabine and its L-carnitine ester derivative(2’-deoxy-2’, 2’-difluoro-N-((4-amino-4-oxobutanoyl) oxy)-4-(trimethyl amm-onio) butanoate-cytidine, JDR) in rat plasma was developed and validated.The conventional plasma sample preparation method of nucleoside analogues is solidphase extraction(SPE) which is time-consuming and cost-expensive. In this study, gradient elution with small particles size solid phase was applied to effectively separate gemcitabine and JDR, and protein precipitation pretreatment was adopted to remove plasma protein and extract the analytes with high recovery(>81%). Method validation was performed as per the FDA guidelines, and the standard curves were found to be linear in the range of 5–4000 ng/ml for JDR and 4–4000 ng/ml for gemcitabine, respectively. The lower limit of quantitation(LLOQ)of gemcitabine and JDR was 4 and 5 ng/ml, respectively. The intra-day and inter-day precision and accuracy results were within the acceptable limits. Finally, the developed method was successfully applied to investigate the pharmacokinetic studies of JDR and gemcitabine after oral administration to rats. 展开更多
关键词 Gemcitabine L-carnitine Prodrug Pharmacokinetics UPLC/MS/MS
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Effect of pasteurization on characteristic aroma substances in mandarin orange(Citrus reticulata cv.Suavissima)juice 认领 引用 被引量:1
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作者 Xuedan Cao Yi Li +5 位作者 Shuangyang Chen Bing Xu Tao Feng Mengfan Zhang Lifang Sun Xiamin Lü 《Journal of Future Foods》 CAS CSCD 2026年第4期604-616,共13页
In this study,headspace solid phase microextraction(SPME),solvent-assisted flavor evaporation(SaFE)and gas chromatography-mass spectrometry were used to detect the changes in aroma components of mandarin orange juice ... In this study,headspace solid phase microextraction(SPME),solvent-assisted flavor evaporation(SaFE)and gas chromatography-mass spectrometry were used to detect the changes in aroma components of mandarin orange juice before and after sterilization,the characteristic flavor substances were also analyzed using odor activity value,principal component analysis,and heatmap.The results showed that 149 volatile components were detected,mainly including terpenes,esters,alcohols,aldehydes,and ketones.Compared with unpasteurized juice,the SPME method measured that 38 new volatile components were produced after pasteurization,while 25 volatile components disappeared;the SAFE method measured the production of 34 volatile components after pasteurization,while 18 volatile components disappeared.β-Myrcene,D-limonene,linalool,and p-cymene were important volatile substances in mandarin orange juice.These results provide a reference for further control and detection of mandarin orange juice processing flavors. 展开更多
关键词 Mandarin orange juice Pasteurizationaroma component Headspace solid phase microextraction Solvent-assisted flavor evaporation
Micromechanical behavior and heterogeneous deformation induced hardening of a novel orientationheterostructured Mg-Y alloy 认领 引用
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作者 Yuliang Yang Yuxin Liu +5 位作者 Zhufeng He Ye Yuan Lifang Sun Hao Zhou Shuang Jiang Nan Jia 《Materials Futures》 EI CSCD 2026年第2期1-23,共23页
Magnesium alloys usually exhibit poor ductility because of their limited slip systems at room temperature.To overcome this intrinsic limitation,heterostructure design has emerged as an effective strategy for enhancing... Magnesium alloys usually exhibit poor ductility because of their limited slip systems at room temperature.To overcome this intrinsic limitation,heterostructure design has emerged as an effective strategy for enhancing their mechanical performance,yet the development of orientation-based heterogeneous magnesium alloys remains relatively unexplored.In this work,by varying the triaxial cyclic compression(TCC)applied to an extruded Mg-2.9Y(wt.%)alloy,we obtained two materials that possessed comparable bimodal grain-size characteristics but showed notable differences in orientation heterogeneity.The material processed by TCC along three orthogonal directions for five complete cycles exhibited a predominantly hard orientation,with hard refined grains embedded within coarse grains of the same hard orientation.By applying an additional compression to plane A,the other material mainly comprising the soft orientation was obtained,with hard-oriented refined grains embedded in soft-oriented coarse grains.These materials exhibited quite different tensile properties and work hardening abilities.By combining microstructural characterization and crystal plasticity modeling,deformation micromechanism of the materials under tensile loading was explored.In the former,poor deformation coordination between the different domains led to strain localization in the refined grain region.However,the latter experienced a significant orientation transition due to tensile twinning.This promoted non-basalslip and improved deformation compatibility,resulting in the more persistent hetero-deformation induced hardening.These findings provide fundamental insights into the micromechanical behavior of heterostructured alloys and offer a new strategy for designing high-performance hexagonal close-packed materials by introducing heterogeneous orientation distributions. 展开更多
关键词 magnesium alloy heterogeneous microstructure orientation crystal plasticity modeling
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