A pyramid sheet has been used in experiments and three-dimensional finite element simulation to study the edge cracks and effects of width on AZ31 sheet under rolling. Results show that the edge cracks of Mg sheet com...A pyramid sheet has been used in experiments and three-dimensional finite element simulation to study the edge cracks and effects of width on AZ31 sheet under rolling. Results show that the edge cracks of Mg sheet comply with the Normalized Crockroft & Latham theory. It can be predicted by D=-0.124+0.09X - 0.008X2, if D ;〉 0, the edge cracks occur. The sheet shearing deformation at the edge brings about cracks. The strain rate changes periodically with different X. With initial width increases, the recrystallized grain size decreases. The finite element model has been validated by the experiment results.展开更多
Parallel mechanisms are extensively employed in industrial logistics,food processing,and medical applications.Due to the strong nonlinearity and cross-axis coupling inherent in closed-chain kinematics,fault diagnostic...Parallel mechanisms are extensively employed in industrial logistics,food processing,and medical applications.Due to the strong nonlinearity and cross-axis coupling inherent in closed-chain kinematics,fault diagnostic performance is highly sensitive to signal perturbations and class imbalance under noisy measurement conditions.Furthermore,diagnostic models trained under single-fault scenarios often exhibit notable performance degradation when transferred to compound fault conditions as a result of distribution shift.In this study,a Delta 3D printer,as a representative parallel mechanism,is adopted as the experimental platform.An interpretable three-stage diagnostic framework is proposed,in which compound fault diagnosis is reformulated as a multi-output classification problem that simultaneously predicts the health states of the A-,B-,and C-belts.This formulation avoids explicit enumeration of compound fault classes while preserving maintenance-relevant,belt-level diagnostic information.Under a strict leakage-avoidance protocol,a fusion ensemble integrating LightGBM and XGBoost classifiers is employed to enhance robustness and generalization to previously unseen compound fault combinations.On the compound-fault subset of the Delta 3D printer dataset,the proposed method achieves a multi-output Macro-F1 score of 09290,with a 95%bootstrap confidence interval of 0.91980.9379.The corresponding belt-wise Macro-F1 scores reach 0.9508,0.9173,and 0.9189 for the A-,B-,and C-belts,respectively.Moreover,the average inference latency on the compound-fault subset is 0.9305 ms per sample,demonstrating a favorable balance between diagnostic accuracy and computational efficiency for edge-deployment scenarios.展开更多
Breast cancer(BC)is one of the most common cancers and the leading causes of death among women worldwide,and its morbidity rate is growing.Discovery of novel biomarkers is necessary for early BC detection,treatment,an...Breast cancer(BC)is one of the most common cancers and the leading causes of death among women worldwide,and its morbidity rate is growing.Discovery of novel biomarkers is necessary for early BC detection,treatment,and prognostication.Circular RNAs(circ RNAs),a novel type of endogenous non-coding RNAs with covalently closed continuous loops,have been found to have a crucial role in tumorigenesis.Studies have demonstrated that circ RNAs are aberrantly expressed in the tumor tissues and plasma of patients with BC,and they modulate gene expression affecting the proliferation,metastasis,and chemoresistance of BC by specifically binding and regulating the expression of micro RNAs(mi RNAs).Therefore,circ RNAs can be used as novel potential diagnostic and prognostic markers,and therapeutic targets for BC.This article summarizes the properties,functions,and regulatory mechanisms of circ RNAs,particularly current research on their association with BC proliferation,metastasis,and chemoresistance.展开更多
BACKGROUND Radiotherapy is widely employed in colorectal cancer(CRC)treatment,but the occurrence of radioresistance severely limits the clinical benefit to patients and significantly contributes to treatment failure a...BACKGROUND Radiotherapy is widely employed in colorectal cancer(CRC)treatment,but the occurrence of radioresistance severely limits the clinical benefit to patients and significantly contributes to treatment failure and recurrent metastasis.AIM To explore the role and underlying mechanism of the lncRNA FTX in radiotherapy resistance in CRC.METHODS LncRNA FTX expression in colorectal parent cells(HT29 and HCT116)and radioresistant cells(HT29R and HCT116R)was determined by real-time quantitative PCR,and the viability of HT29R-shFTX and HCT116R-shFTX cells under ionizing radiation was evaluated using the cell counting kit-8 assay and colony formation experiment.The levels of glutathione and reactive oxygen species in cells after irradiation were determined,and the association between ferroptosis and lncRNA FTX expression in cancer cells was tested.A dual-luciferase assay was used to validate gene interactions.A xenotransplantation mouse model was established to explore the effects of FTX on the CRC tumor radiosensitivity in vivo.RESULTS FTX was upregulated in radioresistant CRC cells,and FTX knockdown inhibited cell survival and increased cell ferroptotic death in response to ionizing radiation.Moreover,lncRNA FTX restricted the SLC7A11 expression by sponging with miR-625-5p,and inhibition of the lncRNA FTX or SLC7A11 significantly increased cellular oxidant levels and DNA damage to ionizing radiation in cancer cells.However,SLC7A11 overexpression reversed the effects of decreased FTX levels on ferroptosis and high oxidation levels in cancer cells exposed to ionizing radiation.CONCLUSION Inhibition of the lncRNA FTX/miR-625-5p/SLC7A11 axis can induce ferroptosis and disturb intracellular redox balance,further sensitizing CRC cells to ionizing radiation,suggesting its potential as a therapeutic target for improving CRC response to radiation therapy.展开更多
基金supported by 973 National Grand Theoretical Research Program(No.2007CB613700)National Sci & Technol Support Program (No.2007BAG06B04)+1 种基金Chongqing Sci & Technol Program (CSTC,2009AB4008)National Natural Science Foundation of China (No.50725413)
摘要A pyramid sheet has been used in experiments and three-dimensional finite element simulation to study the edge cracks and effects of width on AZ31 sheet under rolling. Results show that the edge cracks of Mg sheet comply with the Normalized Crockroft & Latham theory. It can be predicted by D=-0.124+0.09X - 0.008X2, if D ;〉 0, the edge cracks occur. The sheet shearing deformation at the edge brings about cracks. The strain rate changes periodically with different X. With initial width increases, the recrystallized grain size decreases. The finite element model has been validated by the experiment results.
基金funded by Guangdong University Research Project with Grant No.2022KTSCX219Yuejiao Gao Han with Grant No.[2021]4.
摘要Parallel mechanisms are extensively employed in industrial logistics,food processing,and medical applications.Due to the strong nonlinearity and cross-axis coupling inherent in closed-chain kinematics,fault diagnostic performance is highly sensitive to signal perturbations and class imbalance under noisy measurement conditions.Furthermore,diagnostic models trained under single-fault scenarios often exhibit notable performance degradation when transferred to compound fault conditions as a result of distribution shift.In this study,a Delta 3D printer,as a representative parallel mechanism,is adopted as the experimental platform.An interpretable three-stage diagnostic framework is proposed,in which compound fault diagnosis is reformulated as a multi-output classification problem that simultaneously predicts the health states of the A-,B-,and C-belts.This formulation avoids explicit enumeration of compound fault classes while preserving maintenance-relevant,belt-level diagnostic information.Under a strict leakage-avoidance protocol,a fusion ensemble integrating LightGBM and XGBoost classifiers is employed to enhance robustness and generalization to previously unseen compound fault combinations.On the compound-fault subset of the Delta 3D printer dataset,the proposed method achieves a multi-output Macro-F1 score of 09290,with a 95%bootstrap confidence interval of 0.91980.9379.The corresponding belt-wise Macro-F1 scores reach 0.9508,0.9173,and 0.9189 for the A-,B-,and C-belts,respectively.Moreover,the average inference latency on the compound-fault subset is 0.9305 ms per sample,demonstrating a favorable balance between diagnostic accuracy and computational efficiency for edge-deployment scenarios.
基金supported by the Shanghai Municipal Science and Technology Commission,China(Grant No.17411967200)。
摘要Breast cancer(BC)is one of the most common cancers and the leading causes of death among women worldwide,and its morbidity rate is growing.Discovery of novel biomarkers is necessary for early BC detection,treatment,and prognostication.Circular RNAs(circ RNAs),a novel type of endogenous non-coding RNAs with covalently closed continuous loops,have been found to have a crucial role in tumorigenesis.Studies have demonstrated that circ RNAs are aberrantly expressed in the tumor tissues and plasma of patients with BC,and they modulate gene expression affecting the proliferation,metastasis,and chemoresistance of BC by specifically binding and regulating the expression of micro RNAs(mi RNAs).Therefore,circ RNAs can be used as novel potential diagnostic and prognostic markers,and therapeutic targets for BC.This article summarizes the properties,functions,and regulatory mechanisms of circ RNAs,particularly current research on their association with BC proliferation,metastasis,and chemoresistance.
基金Supported by the National Natural Science Foundation of China,No.81960506Science and Technology Fund Project of Guizhou Provincial Health Commission,No.gzwkj2022-299Key Projects of Zunyi Science and Technology Fund,No.zunshikeheHZzi(2023)24 and No.zunshikeheHZzi(2024)9.
摘要BACKGROUND Radiotherapy is widely employed in colorectal cancer(CRC)treatment,but the occurrence of radioresistance severely limits the clinical benefit to patients and significantly contributes to treatment failure and recurrent metastasis.AIM To explore the role and underlying mechanism of the lncRNA FTX in radiotherapy resistance in CRC.METHODS LncRNA FTX expression in colorectal parent cells(HT29 and HCT116)and radioresistant cells(HT29R and HCT116R)was determined by real-time quantitative PCR,and the viability of HT29R-shFTX and HCT116R-shFTX cells under ionizing radiation was evaluated using the cell counting kit-8 assay and colony formation experiment.The levels of glutathione and reactive oxygen species in cells after irradiation were determined,and the association between ferroptosis and lncRNA FTX expression in cancer cells was tested.A dual-luciferase assay was used to validate gene interactions.A xenotransplantation mouse model was established to explore the effects of FTX on the CRC tumor radiosensitivity in vivo.RESULTS FTX was upregulated in radioresistant CRC cells,and FTX knockdown inhibited cell survival and increased cell ferroptotic death in response to ionizing radiation.Moreover,lncRNA FTX restricted the SLC7A11 expression by sponging with miR-625-5p,and inhibition of the lncRNA FTX or SLC7A11 significantly increased cellular oxidant levels and DNA damage to ionizing radiation in cancer cells.However,SLC7A11 overexpression reversed the effects of decreased FTX levels on ferroptosis and high oxidation levels in cancer cells exposed to ionizing radiation.CONCLUSION Inhibition of the lncRNA FTX/miR-625-5p/SLC7A11 axis can induce ferroptosis and disturb intracellular redox balance,further sensitizing CRC cells to ionizing radiation,suggesting its potential as a therapeutic target for improving CRC response to radiation therapy.