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Phylogenetic Analysis of the Dengue Virus Strains Causing the 2019 Dengue Fever Outbreak in Hainan,China 认领 引用 被引量：5
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作者 Jiang Du Liyuan Zhang +16 位作者 Xiaoyuan Hu Ruoyan Peng Gaoyu Wang Yi Huang Wenqi Wang Kunliang Wu Qiang Wang Haoxiang Su Fan Yang Yun Zhang Chuanning Tang Xiuji Cui Lina Niu Gang Lu Meifang Xiao Yongguo Du Feifei Yin 《Virologica Sinica》 SCIE CAS CSCD 2021年第4期636-643,共8页
Dengue virus is an arthropod-borne pathogen that is transmitted to humans primarily by Aedes spp.mosquitos,causing the acute infectious disease,dengue fever(DF).Until 2019,no dengue outbreak had been reported in Haina... Dengue virus is an arthropod-borne pathogen that is transmitted to humans primarily by Aedes spp.mosquitos,causing the acute infectious disease,dengue fever(DF).Until 2019,no dengue outbreak had been reported in Hainan Province for over 20 years.However,in early September of 2019,an increasing number of infected cases appeared and the DF outbreak lasted for over one month in Haikou City,Hainan Province.In our study,we collected 97 plasma samples from DF patients at three hospitals,as well as 1585 mosquito larvae samples from puddles in different areas of Haikou.There were 49(50.5%)plasma samples found to be strongly positive and 9(9.3%)plasma samples were weakly positive against the NS1 antigen.We discovered DENV both in the patient's plasma samples and mosquito larvae samples,and isolated the virus from C6/36 cells inoculated with the acute phase serum of patients.Phylogenetic analysis revealed that the new strains were the most closely related to the epidemic strain in the southern regions of China,belonging to lineageⅣ,genotypeⅠ,DENV-1.Compared to the seven closest strains from neighboring countries and provinces,a total of 18 amino acid mutations occurred in the coding sequences(CDS)of the new isolated strain,DENV1 HMU-HKU-2.Our data shows that dengue virus is re-emerged in Hainan,and pose new threats for public health.Thus regular molecular epidemiological surveillance is necessary for control and prevention of DENV transmission. 展开更多
关键词 DENV-1 Aedes spp.mosquitos Hainan Province Dengue fever patients Mosquito larvae
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Diversity and independent evolutionary profiling of rodent-borne viruses in Hainan,a tropical island of China 认领 引用 被引量：4
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作者 Youyou Li Chuanning Tang +23 位作者 Yun Zhang Zihan Li Gaoyu Wang Ruoyan Peng Yi Huang Xiaoyuan Hu Henan Xin Boxuan Feng Xuefang Cao Yongpeng He Tonglei Guo Yijun He Haoxiang Su Xiuji Cui Lina Niu Zhiqiang Wu Jian Yang Fan Yang Gang Lu Lei Gao Qi Jin Meifang Xiao Feifei Yin Jiang Du 《Virologica Sinica》 SCIE CAS CSCD 2023年第5期651-662,共12页
The risk of emerging infectious diseases(EID)is increasing globally.More than 60%of EIDs worldwide are caused by animal-borne pathogens.This study aimed to characterize the virome,analyze the phylogenetic evolution,an... The risk of emerging infectious diseases(EID)is increasing globally.More than 60%of EIDs worldwide are caused by animal-borne pathogens.This study aimed to characterize the virome,analyze the phylogenetic evolution,and determine the diversity of rodent-borne viruses in Hainan Province,China.We collected 682 anal and throat samples from rodents,combined them into 28 pools according to their species and location,and processed them for next-generation sequencing and bioinformatics analysis.The diverse viral contigs closely related to mammals were assigned to 22 viral families.Molecular clues of the important rodent-borne viruses were further identified by polymerase chain reaction for phylogenetic analysis and annotation of genetic characteristics such as arenavirus,coronavirus,astrovirus,pestivirus,parvovirus,and papillomavirus.We identified pestivirus and bocavirus in Leopoldoms edwardsi from Huangjinjiaoling,and bocavirus in Rattus andamanensis from the national nature reserves of Bangxi with low amino acid identity to known pathogens are proposed as the novel species,and their rodent hosts have not been previously reported to carry these viruses.These results expand our knowledge of viral classification and host range and suggest that there are highly diverse,undiscovered viruses that have evolved independently in their unique wildlife hosts in inaccessible areas. 展开更多
关键词 Rodents Virome Phylogenetic analysis Novel viruses Wildlife
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Immunomodulatory effect of Dictyophora indusiate protease hydrolysate through TLR4/NF-κB pathway and restoration of intestinal barrier integrity 认领 引用
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作者 Meifang Xiao Pengyi Wang +5 位作者 Zihui Chen Ying Chen Quancen Li Bin Liu Xin Li Feng Zeng 《Food Bioscience》 SCIE 2026年第2期631-643,共13页
Dictyophora indusiate(DI)contains numerous bioactive compounds with immunomodulatory properties.However,the functional activities of its protein hydrolysate and their mechanisms of action in the regulation of systemic... Dictyophora indusiate(DI)contains numerous bioactive compounds with immunomodulatory properties.However,the functional activities of its protein hydrolysate and their mechanisms of action in the regulation of systemic and intestinal mucosal immunity remain poorly understood.This study comprehensively evaluated the immunomodulatory potential of D.indusiate peptide hydrolysate(DIPH)using a multi-omics approach.LC-MS/MS analysis showed that DIPH is composed of peptides containing 8 to 20 amino acid residues,with branchedchain amino acids comprising 20.53%of the total,and exhibits primarily hydrophobic properties.In cyclophosphamide-induced immunosuppressed mice,DIPH exhibited broad restorative effects on immunity via three interrelated mechanisms.Firstly,systemic immune function was enhanced,as demonstrated by significant elevations in serum IgG and IgM levels,and an improvement(4.5-fold)in carbon clearance capacity.Secondly,intestinal barrier integrity was strengthened through the upregulation of tight junction proteins,a 24.7%rise in goblet cells,and a 1.11-fold increase in secretory IgA production.Thirdly,DIPH administration modulated gut microbiota composition,significantly enriching beneficial bacteria including Bifidobacterium and Roseburia while reducing Streptococcus,which was accompanied by a 2.1-fold increase in butyrate production.At the molecular level,DIPH exerted its effects through dual regulatory mechanisms:suppression of the TLR4/MyD88/NF-κB pathway and activation of FoxO1-mediated mucosal restoration.These insights collectively establish DIPH as a multi-target edible fungi-derived immunomodulator that uniquely integrates gut microbiota regulation,systemic immune enhancement,and intestinal barrier restoration,highlighting its promising applications in functional food development and microbiome-targeted therapies. 展开更多
关键词 Dictyophora indusiate Protein hydrolysate Immunostimulatory activity Intestinal mucosal immunity
Lactiplantibacillus plantarum CN2018 enhances the milk fermentation characteristics by improving antioxidant capacity and enhancing the milk flavor profile 认领 引用 被引量：2
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作者 Hao Gao Meifang Xiao +5 位作者 Hang Guo Leilei Yu Fengwei Tian Qixiao Zhai Wei Chen Hao Zhang 《Food Bioscience》 SCIE 2025年第1期1299-1310,共12页
Lactiplantibacillus plantarum has been extensively studied for its probiotic properties.Previous studies have demonstrated that L.plantarum CN2018 exhibited antioxidant properties and the potential to inhibit Helicoba... Lactiplantibacillus plantarum has been extensively studied for its probiotic properties.Previous studies have demonstrated that L.plantarum CN2018 exhibited antioxidant properties and the potential to inhibit Helicobacter pylori.This research aimed to further investigate the fermentation potential of L.plantarum CN2018 as a cofermenter in the production of fermented milk,including fermentation characteristics,textural properties,antioxidant capacity,flavor compounds and the metabolic changes.The addition of CN2018 significantly influenced the fermentation characteristics,resulting in a reduction in fermentation time and an increase in the total viable bacterial count(1.41×109 CFU/mL).The textural properties of chewiness,gumminess,and adhesiveness improved by 15.29%(p<0.05),20.05%(p<0.001),and 44.87%(p<0.05),respectively.Additionally,CN2018 enhanced the antioxidant capacity of fermented milk,which SOD activity,T-AOC and DPPH scavenging were increased by 51.78%(p<0.05),80.46%(p<0.05)and 43.58%(p<0.01),respectively.Key flavor compounds including 1-(4-methylphenyl)-ethanone,phthalic acid,hex-3-yl isobutyl ester,(E)-2-decenal,and phenylethanol were identified,thereby enhancing the flavor profile of the fermented milk.Metabolomic analysis demonstrated that hypoxanthine,a compound associated with oxidative stress,significantly reduced in fermented milk of CN2018.This may be associated with the enhanced antioxidant capacity of fermented milks.These findings indicate that CN2018 can effectively degrade or absorb hypoxanthine in fermented milk.Overall,L.plantarum CN2018 demonstrates considerable potential as an auxiliary fermenter to enhance the nutritional and functional properties of fermented dairy products. 展开更多
关键词 Fermented milk Lactiplantibacillus plantarum CN2018 Hypoxanthine Antioxidant capacity Untargeted metabolomics
Latilactobacillus sakei CCFM1267 enhances fermentation and probiotic properties in fermented milk:A focus on metabolomics and physicochemical analysis 认领 引用 被引量：1
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作者 Yuxuan Xia Xing Jin +7 位作者 Meifang Xiao Zi Ye Chengcheng Zhang Jianxin Zhao Wei Chen Fengwei Tian Leilei Yu Qixiao Zhai 《Food Bioscience》 SCIE 2025年第3期1541-1550,共10页
Latilactobacillus sakei has demonstrated significant potential as a probiotic in fermented foods;however,its role and impact as a co-fermenting agent in dairy products remain largely unexplored.This study investigated... Latilactobacillus sakei has demonstrated significant potential as a probiotic in fermented foods;however,its role and impact as a co-fermenting agent in dairy products remain largely unexplored.This study investigated the effects of L.sakei CCFM1267 on the fermentation properties,physicochemical characteristics,and metabolite profiles of fermented milk.Two batches of fermented milk were prepared:one containing only Lactobacillus delbrueckii subsp.bulgaricus and Streptococcus thermophilus(BS),and the other containing L.bulgaricus,S.thermophilus and L.sakei(BSLs).Samples were collected during the 16-h fermentation period and at storage times of 2,7 and 14 days for analyses of pH,water holding capacity(WHC),texture,viable bacterial counts,free amino acids(FAAs),volatile flavor compounds,and metabolomics analysis using untargeted approaches such as high-performance liquid chromatography-mass spectrometry(HPLC-MS).The addition of L.sakei CCFM1267 accelerated the fermentation process,resulting in consistently higher viable cell counts(maintaining levels above 106 CFU/mL)during fermentation and storage,improved WHC(p<0.01),and enhanced viscosity(p<0.05).Metabolomic analyses revealed significant increases in various FAAs(e.g.,phenylalanine,leucine,valine,lysine,methionine,glutamic acid),ketone flavor compounds,and benzoic acid in the BSLs group.Notably,the BSLs fermented milk contained higher levels of acetylcholine and glycylproline,which are associated with antiinflammatory and immune response functions,respectively.These findings highlight the potential of L.sakei CCFM1267 as a valuable co-fermenting agent for developing fermented dairy products with enhanced nutritional value,sensory quality,and health-promoting properties. 展开更多
关键词 Latilactobacillus sakei CCFM1267 Fermented milk Metabolomics Physicochemical properties Flavor compounds Bioactive peptides
The alleviating effect of different bile salt hydrolase gene-encoding Limosilactobacillus fermentum strains on hypercholesterolemia in mice 认领 引用
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作者 Mengnan Zhou Meifang Xiao +8 位作者 Hang Guo Xinyu Guo Xiaojie Zang Qingsong Zhang Leilei Yu Jianxin Zhao Qixiao Zhai Fengwei Tian Wei Chen 《Food Bioscience》 SCIE 2025年第6期3156-3169,共14页
This work aimed to investigate the impact of different bile salt hydrolase(BSH)distributions on the cholesterollowering capacity of Limosilactobacillus fermentum through comparative genomics,molecular docking simulati... This work aimed to investigate the impact of different bile salt hydrolase(BSH)distributions on the cholesterollowering capacity of Limosilactobacillus fermentum through comparative genomics,molecular docking simulations,and in vitro and in vivo experiments.BSH is considered an important characteristic for probiotics to lower serum total cholesterol(TC).Strains with different BSH distributions possess varying abilities to hydrolyze bile salts.L.fermentum has been demonstrated to exhibit significant cholesterol-lowering potential and may alleviate hypercholesterolemia.In this study,three types of BSHs were identified across 219 strains of L.fermentum,and molecular simulations revealed that BSH3 formed the most stable binding with substrates.Based on the distribution patterns of BSHs,L.fermentum strains were classified into three types:L.fermentum I(containing BSH2),L.fermentum II(containing BSH1 and BSH2),and L.fermentum III(containing BSH1,BSH2,and BSH3).In vitro experiments involving 34 strains of L.fermentum demonstrated notable variations in bile salt hydrolysis and cholesterol-lowering capability across different types,with L.fermentum III showing superior performance compared to the other two types.After 12 weeks of a high-cholesterol diet(HCD),C57BL/6 N mice treated with L.fermentum III,II,and I showed serum TC reductions of 27.19%,21.65%,and 25.15%;low-density lipoprotein cholesterol(LDL-C)reductions of 25.06%,8.11%,and 9.41%;and increased fecal total bile acids(TBAs)excretion by 10.97%,7.24%,and 6.02%,respectively.Overall,the alleviating effect of L.fermentum III was markedly superior to the other two types.This indicates the important role of BSHs in the alleviation of hypercholesterolemia by L.fermentum,providing a valuable perspective for screening probiotics capable of alleviating hypercholesterolemia. 展开更多
关键词 Limosilactobacillus fermentum Bile salt hydrolase Cholesterol Hypercholesterolemia
Therapeutic potential of Latilactobacillus curvatus CCFM1268 in colitis treatment:Insights from in vitro and in vivo studies 认领 引用 被引量：1
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作者 Meifang Xiao Yuxuan Xia +7 位作者 Ying Chen Shuan Wang Jianxin Zhao Arjan Narbad Wei Chen Qixiao Zhai Leilei Yu Fengwei Tian 《Food Bioscience》 SCIE 2024年第3期2048-2057,共10页
Latilactobacillus curvatus is a candidate probiotic that is known to have antioxidant,immunomodulatory,anti-obesity,and colitis-relieving beneficial effects.Here,we evaluated the effects of Lt.curvatus CCFM1268 on dex... Latilactobacillus curvatus is a candidate probiotic that is known to have antioxidant,immunomodulatory,anti-obesity,and colitis-relieving beneficial effects.Here,we evaluated the effects of Lt.curvatus CCFM1268 on dextran sulfate sodium(DSS)-induced Caco-2 cell damage and colitis in mice.Our results demonstrate that Lt.curvatus CCFM1268 significantly restored cell viability and promoted the expression of barrier factors in vitro.In vivo,Lt.curvatus CCFM1268 significantly restored weight loss and colon damage in mice and modulated the levels of inflammatory factors and tight junction proteins in the colon.Latilactobacillus curvatus CCFM1268 also reduced the abundance of conditionally pathogenic bacteria including Escherichia_shigella and Alistipes,enriched the abundance of Lactobacillus,Parabacteroides,Enterococcus and Faecalibaculum,and significantly increased the concentrations of acetic,propionic,and butyric acid in the gut.These results indicate that Lt.curvatus CCFM1268 can alleviate colitis by inhibiting colonic inflammation,repairing the gut barrier,regulating the gut microbiota composition,and secreting metabolites,which was demonstrated both in vivo and in vitro. 展开更多
关键词 Latilactobacillus curvatus Probiotic Cytokines Experimental colitis
肠道菌群的聚糖利用特性主导其交叉喂养网络:聚焦双歧杆菌 认领 引用 被引量：7
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作者 于雷雷 肖嵋方 +2 位作者 田丰伟 陈卫 翟齐啸 《Science Bulletin》 SCIE EI CAS CSCD 2024年第3期299-302,共4页
The diverse carbon sources in the gut tract provide various nutritional niches for the gut microbiota(GM).Differential glycan utilization influences GM formation in these niches[1].Bifidobacteria are key microorganism... The diverse carbon sources in the gut tract provide various nutritional niches for the gut microbiota(GM).Differential glycan utilization influences GM formation in these niches[1].Bifidobacteria are key microorganisms in the human gut,as they maintain a long-term symbiotic relationship with their hosts,which is essential for gut homeostasis and health.The high abundance and colonization of Bifidobacteria in the gut are closely related to their strong ability to degrade human milk oligosaccharides(HMOs)and dietary glycans. 展开更多
关键词 homeostasis maintain dietary
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