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Cytoprotective activity of Pogonatherum paniceum(Lam.)Hack.ethanolic extract evaluated by synchrotron radiation-based Fourier transform infrared microspectroscopy 认领 引用
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作者 Benjawan Dunkhunthod Kanjana Thumanu +2 位作者 Yothin Teethaisong Priyada Sittisart Patcharawan Sittisart 《Journal of Integrative Medicine》 SCIE CAS CSCD 2025年第2期182-194,共13页
Objective:The present study investigated the cytoprotective effects of a Pogonatherum paniceum extract prepared with 80%ethanol(PPE)using synchrotron radiation-based Fourier transform infrared(SR-FTIR)microspectroscop... Objective:The present study investigated the cytoprotective effects of a Pogonatherum paniceum extract prepared with 80%ethanol(PPE)using synchrotron radiation-based Fourier transform infrared(SR-FTIR)microspectroscopy and determined its phytochemical profile.Methods:The volatile and polyphenolic compounds in PPE were characterized using gas chromatography–mass spectrometry and liquid chromatography–mass spectrometry,respectively.The antioxidant capacity of PPE was evaluated using chemical and cell-based assays.The SR-FTIR microspectroscopy was performed to evaluate the cytoprotective effect of PPE by identifying changes in macromolecule composition in tert-butyl hydroperoxide(t BuOOH)-induced oxidative damage in RAW264.7 cells.Results:A total of 48 volatile compounds and 28 polyphenol components were found in PPE.PPE exhibited a high potential for antioxidant activity by scavenging the intracellular reactive oxygen species in t Bu OOH-induced oxidative damage in RAW264.7 cells.PPE treatment also significantly protected RAW264.7 cells against t BuOOH-induced toxicity and restored cell viability.The SR-FTIR analysis revealed that t BuOOH increased the lipid and ester lipid content in RAW264.7 cells.The PPE exerted a cytoprotective effect by decreasing the levels of lipid and ester lipid compounds that had been elevated by t BuOOH in RAW264.7 cells.These findings indicate that PPE has cytoprotective potential due to its ability to inhibit endogenous reactive oxygen species.Conclusion:This study extends the current knowledge on the phytochemistry of PPE and its antioxidant and cytoprotective effects.These findings support the use of SR-FTIR microspectroscopy to determine the cytoprotective effects of natural products.PPE extract may be a candidate compound for new therapeutics and nutraceuticals that target the prevention of oxidative stress-associated diseases. 展开更多
关键词 Pogonatherum paniceum Reactive oxygen species Antioxidant Phytochemical Cytoprotective Synchrotron radiation-based Fourier transform infrared microspectroscopy
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Mode of action of biosurfactant against Listeria monocytogenes and its cytotoxicity as an alternative for washing fresh Chinese kale 认领 引用
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作者 Priyada Sittisart Piyawan Gasaluck +4 位作者 Benjawan Dunkhunthod Thitikorn Mahidsanan Janjira Pinyo Yothin Teethaisong Patcharawan Sittisart 《Food Bioscience》 SCIE 2024年第5期1099-1108,共10页
Biosurfactants play a very important role in the fresh produce industry,especially as anti-microbial agents.This study aimed to investigate the anti-oxidant and anti-bacterial activities of a glycolipoprotein biosurfa... Biosurfactants play a very important role in the fresh produce industry,especially as anti-microbial agents.This study aimed to investigate the anti-oxidant and anti-bacterial activities of a glycolipoprotein biosurfactant(BSF)produced by Lactobacillus plantarum MGL-8,as well as its anti-bacterial mechanism of action against Listeria monocytogenes DMST17303.Its application as a sanitizer for fresh Chinese kale was investigated at different storage temperatures and exposure times.Its safety was monitored by cytotoxic effect to human skin HaCaT keratinocyte cells.The BSF exhibited high anti-oxidant capacities,with ferric-reducing from the FRAP assay of 15.96μgVCEA/mg and ABTS scavenging activities with an IC50 of 877.00μg/mL.BSF demonstrated anti-bacterial activity against L.monocytogenes through decreasing the fluidity and increasing permeability of the cell membrane,resulting in reducing membrane function and leakage of intracellular components.The study revealed that the appropriate washing procedures of W2(BSF 400μg/mL for 20 min)and W3(BSF 450μg/mL for 10 min)efficiently inhibited the growth of L.monocytogenes in fresh Chinese kale stored at 5℃for 7 and 14 days.BSF did not exhibit any cytotoxic effects on HaCaT cells at concentrations of up to 1,000μg/mL,suggesting using BSF for disinfect L.monocytogenes in fresh Chinese kale at 450μg/mL is safe for human.Therefore,it is recommended to wash fresh Chinese kale with BSF at 400 or 450μg/mL for 20 or 10 min,respectively,to reduce food safety risks such as L.monocytogenes before storing at 5℃for 14 days. 展开更多
关键词 Biosurfactant Fresh Chinese kale Washing Listeria monocytogenes Anti-oxidant Cytotoxicity
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