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Novel 3D bioprinting approach for spinal cord injury repair using neural stem cells and TGF-β1 monoclonal antibody 认领 引用
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作者 Ming Deng Geliang Hu +8 位作者 Fan Liang Qi Liao Wanjun Ding Bin He Jianghua Ming Hui Huang Junqi Liu Zhaohong Kong Ping Xie 《Nano Materials Science》 EI CAS CSCD 2026年第3期625-640,共16页
Objective:To investigate the repair of spinal cord injury(SCI)in rats using 3D bioprinted decellularized extracellular matrix(dECM)hydrogel,neural stem cells(NSCs),and TGF-β1 monoclonal antibody.Methods:The spinal co... Objective:To investigate the repair of spinal cord injury(SCI)in rats using 3D bioprinted decellularized extracellular matrix(dECM)hydrogel,neural stem cells(NSCs),and TGF-β1 monoclonal antibody.Methods:The spinal cord-derived dECM hydrogel(SC-dECM-gel)was optimized for 3D printing.Rheological tests established a 3%concentration as optimal.In vitro tests assessed the effects of TGF-β1 monoclonal antibody on NSC viability and differentiation.A rat SCI model was treated with the printed gel,and recovery was monitored using the Basso-Beattie-Bresnahan test and evoked potentials.Results:The 3%SC-dECM-gel showed superior rheological properties.TGF-β1 monoclonal antibody enhanced NSC survival and differentiation in vitro.In vivo,the 3D bioprinted gel significantly improved motor function and promoted neuronal regeneration.Conclusion:3D bioprinted SC-dECM-gel loaded with NSCs and TGF-β1 monoclonal antibody effectively restores motor function and promotes regeneration in SCI rats,offering a promising approach for SCI treatment. 展开更多
关键词 Spinal cord injury 3D bioprinting Decellularized extracellular matrices Neural stem cells Transforming growth factor-beta 1 monoclonal antibody Neural regeneration Functional recovery
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Influence of delay time on the blasting effect of multiple charging forms in single free-face rock masses 认领 引用
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作者 Chengxiao Li Renshu Yang +2 位作者 Jinjing Zuo Ye Zhu Ping Xie 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2026年第6期1807-1822,共16页
ln order to improve excavation efficiency,we considered coal mine rock roadway blasting excavation as a background to examine the influence of delay time on the blasting effect of different charging structures under s... ln order to improve excavation efficiency,we considered coal mine rock roadway blasting excavation as a background to examine the influence of delay time on the blasting effect of different charging structures under single free-surface conditions.Single-pore dispersed-charge models,dual-pore continuous-charge models,and dual-pore composite charge models were established.Their respective explosive rock-breaking mechanisms were explained using different models.These three numerical models were used to analyze the influence of delay time changes on the pressure and velocity of the measurement points near boreholes.The models were used to evaluate the blasting effects by determining the number of free-surface rocks.Engineering experiments were conducted to validate the numerical findings.The results showed that a single-hole dispersed charge creates a cavity and a new free surface,which increases the impact of deep-hole blasting compared to stress wave superposition.Dual-hole continuous-charge detonation is difficult,and short-delay detonation can effectively use stress wave superposition and prolong the action time of the explosive gas.The cavities created by the dispersed charges can be used to increase the efficiency of dual-hole composite charge blasting. 展开更多
关键词 underground mine blasting excavation delay blasting blasting cavity dispersed charge free surface
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A preclinical mouse model mimicking the ovarian cancer-induced estrogen deficiency-depression axis 认领 引用
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作者 Jiamin Liu Yafei Yang +4 位作者 Chunxi Liu Danting Wen Tingyu Zhao Ping Xie Jing Xiao 《Animal Models and Experimental Medicine》 CAS CSCD 2026年第5期853-865,共13页
Background:Young ovarian cancer(OC)patients often experience a synergistic inter-play among tumor progression,estrogen deficiency,and depression,which severely compromises prognosis and quality of life.However,a precl... Background:Young ovarian cancer(OC)patients often experience a synergistic inter-play among tumor progression,estrogen deficiency,and depression,which severely compromises prognosis and quality of life.However,a preclinical model that faithfully recapitulates this triad remains lacking.This study aimed to develop and validate a novel mouse model to investigate the interrelationship between ovarian cancer,hy-poestrogenic states,and depression.Methods:An ovarian cancer–related depression(OCRD)model was established by integrating previously validated components of tumor-depression paradigms,includ-ing ID-8 ovarian cancer modeling and chronic restraint stress-induced depressive-like behavior.Orthotopic(intraperitoneal)and heterotopic(subcutaneous)tumor models were combined with chronic stress.Estrogen status was manipulated by ovary re-tention or ovariectomy(OVX),performed either 1 day before tumor implantation to model preexisting estrogen deficiency or 7 days after engraftment to mimic treatment-induced abrupt hormonal loss.Model validity was assessed using behavioral assays(sucrose preference test,tail suspension test,and open field test),neuroendocrine profiling,and longitudinal evaluation of tumor progression.Results:The model generated by intraperitoneal tumor engraftment,OVX,and chronic stress exhibited pronounced depressive-like behavior,accompanied by marked es-trogen depletion and accelerated tumor progression.All mice survived throughout the experimental period,indicating high model stability and reproducibility.This inte-grated phenotype closely mirrors key clinical features observed in young OC patients undergoing abrupt estrogen deprivation.Conclusions:The combination of intraperitoneal ovarian cancer modeling,ovariectomy,and chronic stress induction establishes a stable and clinically relevant preclinical model of hypoestrogenic ovarian cancer–related depression,suitable for translational research. 展开更多
关键词 chronic restraint stress hypoestrogenism mice model ovarian cancer–related depression ovariectomy
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Mechanism of loop-2 in facilitating microtubule depolymerase activity of kinesin-8 motors 认领 引用
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作者 Xiao-Xuan Shi Yao Wang +2 位作者 Jie Wang Yu-Ru Liu Ping Xie 《Chinese Physics B》 SCIE EI CAS CSCD 2026年第2期647-658,共12页
Kinesin-8 motors can move with a high processivity on microtubule lattices toward the plus end.After reaching the plus end,the kinesin-8 motors can pause for a long time and promote the microtubule depolymerization.He... Kinesin-8 motors can move with a high processivity on microtubule lattices toward the plus end.After reaching the plus end,the kinesin-8 motors can pause for a long time and promote the microtubule depolymerization.Here,using atomistic molecular dynamics simulations we studied the structural changes of the kinesin-8 head in different nucleotide states bound to the straight and curved tubulins and the corresponding interactions between them.We found that the kinesin-8 head in ATP and/or ADP–Pi state has the similar strong affinity while in ADP state has the similar weak affinity to both the straight and curved tubulins,which is strongly implicated in the mechanism of the long but very different residence times of the kinesin-8 motor on the microtubule lattice and at the end.Moreover,we found that loop-2 of the kinesin-8 head bound strongly to the curved tubulin in the stable state has a large interference with its neck linker pulled in the minusended orientation.This is contrary to the case of the head bound strongly to the straight tubulin,where loop-2 has little interference with its neck linker pulled in the minus-ended orientation.The large interference can induce a larger internal force between the two heads and thus can induce the two curved tubulins bound strongly by the two heads to be more curved relative to each other.This is strongly implicated in the mechanism of the depolymerase activity of the kinesin-8motors and explains the origin of loop-2 playing a facilitating role in the depolymerase activity. 展开更多
关键词 microtubule depolymerization kinesin binding energy straight tubulin curved tubulins molecular dynamics simulation
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多孔直管式进气气-液搅拌槽内气液分散特性 认领 引用
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作者 孙睿彧 谢萍 +3 位作者 蔡子琦 刘新卫 高正明 包雨云 《过程工程学报》 CAS CSCD 北大核心 2026年第4期372-380,共9页
气-液搅拌槽在氧化、加氢等化工过程中具有广泛应用,其气体分散状态直接影响生产效率,而搅拌桨型、桨叶安装高度及进气结构是气液分散的重要影响因素。本工作在具有多孔直管进气结构的气液搅拌槽中考察了宽叶翼型搅拌(WH)桨和半椭圆管涡... 气-液搅拌槽在氧化、加氢等化工过程中具有广泛应用,其气体分散状态直接影响生产效率,而搅拌桨型、桨叶安装高度及进气结构是气液分散的重要影响因素。本工作在具有多孔直管进气结构的气液搅拌槽中考察了宽叶翼型搅拌(WH)桨和半椭圆管涡轮(HEDT)桨的桨叶安装位置和转速等条件对气液分散特性的影响规律。结果表明,多孔直管进气结构可实现较好的气泡分散均匀性,HEDT桨适用于较高转速下的气液分散操作,而WH桨在相对较低的转速下即表现出较好的气液分散效果。对于两种搅拌桨,L/D=0.75均为较优的安装高度。本研究结果可为气-液搅拌槽性能优化和设计提供方法参考,具有显著的实际应用价值。 展开更多
关键词 搅拌槽 多孔管 分布式进气 气液分散
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On load dependence of detachment rate of kinesin motor 认领 引用 被引量：1
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作者 Xiao-Xuan Shi Yao Wang +1 位作者 Yu-Ru Liu Ping Xie 《Chinese Physics B》 SCIE EI CAS CSCD 2025年第1期498-505,共8页
Kinesin is an archetypal microtubule-based molecular motor that can generate force to transport cargo in cells. The load dependence of the detachment rate is an important factor of the kinesin motor, the determination... Kinesin is an archetypal microtubule-based molecular motor that can generate force to transport cargo in cells. The load dependence of the detachment rate is an important factor of the kinesin motor, the determination of which is critically related to the chemomechanical coupling mechanism of the motor. Here, we use three models for the load dependence of the detachment rate of the kinesin motor to study theoretically and numerically the maximal force generated and microtubuleattachment duration of the motor. By comparing the theoretical and numerical results with the available experimental data,we show that only one model can explain well the available experimental data, indicating that only this model can be applicable to the kinesin motor. 展开更多
关键词 kinesin detachment rate generated force chemomechanical coupling mechanism optical trapping
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RAF1 mutation expands the cardiac phenotypic spectrum of Noonan syndrome: A case report 认领 引用
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作者 Nan Ma Zhong-Wei Li +6 位作者 Jia-Jia Liu Xing-Guang Liu Xing Zhou Bo-Wen Wang Yan-Ling Li Tian-Cheng Zhang Ping Xie 《World Journal of Cardiology》 2025年第6期163-172,共10页
BACKGROUND Noonan syndrome is a relatively common autosomal dominant genetic disorder characterized by cardiovascular defects owing to functional abnormalities in key genes such as RAF1.Mutations in RAF1 are typically... BACKGROUND Noonan syndrome is a relatively common autosomal dominant genetic disorder characterized by cardiovascular defects owing to functional abnormalities in key genes such as RAF1.Mutations in RAF1 are typically associated with hypertrophic cardiomyopathy(HCM).However,in this case,the patient exhibited atrial and ventricular septal defects(VSDs).CASE SUMMARY This case report describes an 11-year-old boy diagnosed with Noonan syndrome,in whom genetic testing revealed a c.770C>T(p.Ser257 Leu)mutation in RAF1.The patient presented with intermittent chest discomfort and shortness of breath,symptoms that significantly worsened after physical activity.Clinical evaluation revealed marked growth retardation and multiple physical abnormalities.Electrocardiographic and echocardiographic assessments revealed VSDs,atrial septal defects,and left ventricular outflow tract obstruction.Following multidisciplinary consultation,the patient underwent cardiac surgical intervention,which led to clinical improvement;however,they subsequently developed a third-degree atrioventricular block,necessitating the implantation of a permanent pacemaker.During follow-up,echocardiographic findings demonstrated near-complete resolution of the shunt across the atrial and ventricular septa,significant improvement in left ventricular outflow tract obstruction,and notable reduction in ventricular septal thickness.A genetic mutation at the c.770C>T(p.Ser257 Leu)locus of RAF1 is typically associated with HCM and pulmonary hypertension.However,this patient’s clinical phenotype manifested as HCM,atrial septal defect,and VSD,suggesting that this mutation may involve a different pathophysiological mechanism.CONCLUSION This case confirms the genotype-phenotype heterogeneity of Noonan syndrome and highlights the complex management requirements of RAF1 mutation-associated cardiac pathologies.Early surgical intervention can ameliorate structural defects,but it must be integrated with genetic counseling and lifelong monitoring to optimize patient outcomes. 展开更多
关键词 Noonan syndrome RAF1 gene mutation Hypertrophic cardiomyopathy Atrial septal defect Ventricular septal defect Case report
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Transport of track-interacted cargo by molecular motor 认领 引用
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作者 Xiao-Xuan Shi Yu-Ru Liu Ping Xie 《Communications in Theoretical Physics》 SCIE CAS CSCD 2025年第9期116-128,共13页
Intracellular transports of cargoes are performed by biological molecular motors that move processively along their linear tracks.In some cases,the cargo can interact with the track.A typical example of these cases is... Intracellular transports of cargoes are performed by biological molecular motors that move processively along their linear tracks.In some cases,the cargo can interact with the track.A typical example of these cases is the transport of a major mitotic signaling module,the chromosomal passenger complex(CPC),along the microtubule toward the equatorial cortex by a kinesin-6 motor during anaphase,where the CPC can interact with the microtubule.Here,an analytical theory is presented on the dynamics of the molecular motor transporting a track-interacted cargo.The theory is then applied to the transport of the track-interacted cargo by kinesin-6 and by kinesin-1 motors,with the theoretical results reproducing quantitatively the available experimental data.It is found that a diffusive cargo along the track,with the diffusion constant≥0.1μm2s-1,can largely enhance the processivity relative to the non-diffusive cargo and relative to the cargo having no interaction with the track. 展开更多
关键词 molecular motor intracellular cargo transport kinesin
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Yolk-shell FeSe2@CoSe2/FeSe2 heterojunction as anode materials for sodium-ion batteries with high rate capability and stability 认领 引用 被引量：5
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作者 Liuyang Zhang Bicheng Zhu +4 位作者 Difa Xu Zibao Qian Ping Xie Tao Liu Jiaguo Yu 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2024年第5期185-195,共11页
Sodium-ion batteries are promising candidates for large-scale grid storage systems and other applications.Their foremost advantage derives from superior environmental credentials,enhanced safety as well as lower raw m... Sodium-ion batteries are promising candidates for large-scale grid storage systems and other applications.Their foremost advantage derives from superior environmental credentials,enhanced safety as well as lower raw material costs than lithium-ion batteries.It is still challenging to explore desirable anode material.In this study,FeSe2@CoSe2/FeSe2,with a yolk-shell structure was prepared by ion exchange and selenisation.The FeSe2@CoSe2/FeSe2prepared as anode material for sodiumion batteries exhibits excellent rate capability due to the synergistic effect of bimetallic selenides and the interfacial effect of the heterostructure.Moreover,it delivers high performance(510 mAh g-1at 0.2 A g-1),superior rate capa-bility(90%retention at 5 A g-1),and good long-time cycling stability(78%capacity retention after 1800 cycles at a high current density of 2 A g-1).The optimized sodiumion full cell with FeSe2@CoSe2/FeSe2as the anode and Na 3 V 2(PO 4)3 as the cathode still demonstrates excellent performance.Namely,a ca-pacity of 272 mAh g-1(at 1 A g-1)within the operating voltage from 1 to 3.8 V can be obtained.This work illustrates the potential of bimetallic selenides with heterostructures for performance enhancement of sodium-ion batteries. 展开更多
关键词 Cobalt-iron selenide Heterojunctions Sodium storage Anode material Full battery
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Transfer of the zp3a gene results in changes in egg adhesiveness and buoyancy in transgenic zebrafish 认领 引用 被引量：4
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作者 Yu-Qing Cao Yong-Xin Wang +5 位作者 Yan Zhao Jia Zhang Xian He Ping Xie Jun Chen Yu-Hua Sun 《Zoological Research》 SCIE CSCD 2023年第2期259-268,共10页
Reproductive strategies and spawning habits play key roles in the evolution of endemic East Asian cyprinids.However,the molecular mechanisms underlying the regulation of spawning habits are not well understood.We rece... Reproductive strategies and spawning habits play key roles in the evolution of endemic East Asian cyprinids.However,the molecular mechanisms underlying the regulation of spawning habits are not well understood.We recently identified zona pellucida(Zp) as the top differentially expressed protein between East Asian cyprinids that produce adhesive and semi-buoyant eggs,suggesting that Zp protein may play important roles in the regulation of egg type.In this work,we generated transgenic zebrafish in which oocyte-specific expression of zp genes from rare minnow(Gobiocypris rarus),an East Asian cyprinid laying adhesive eggs,was driven by a zebrafish zp3.2 gene promoter.We found that the transgenic eggs obtained partial adhesiveness and exhibited alteration in hydration and buoyancy.Abnormal metabolism of vitellogenin(VTG) may contribute to enhanced hydration and/or buoyancy.Our work shows that expression of the exogenous zp3a gene from an adhesiveegg producing fish is sufficient to induce changes in both egg adhesiveness and buoyancy in zebrafish,emphasizing the important role of zp genes in the regulation of spawning habits.Our results thus provide new insights into how endemic East Asian cyprinids may have adapted to the Yangtze river-lake system via changes in spawning habits. 展开更多
关键词 Zona pellucida protein Adhesive Buoyancy Hydration Egg envelope
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Decrease of NH_4^+-N by bacterioplankton accelerated the removal of cyanobacterial blooms in aerated aquatic ecosystem 认领 引用 被引量：3
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作者 Xi Yang Ping Xie +3 位作者 Zhimei Ma Qing Wang Huihui Fan Hong Shen 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2013年第11期2223-2228,共6页
We used aerated systems to assess the influence of the bacterioplankton community on cyanobacterial blooms in algae/post-bloom of Lake Taihu, China. Bacterioplankton community diversity was evaluated by polymerase cha... We used aerated systems to assess the influence of the bacterioplankton community on cyanobacterial blooms in algae/post-bloom of Lake Taihu, China. Bacterioplankton community diversity was evaluated by polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE） fingerprinting. Chemical analysis and nitrogen dynamic changes illustrated that NH4＋-N was nitrified to NO2^--N and NO3^--N by bacterioplankton. Finally, NHa^＋-N was exhausted and NO3^--N was denitrified to NO2^--N, while the accumulation of NO2^--N indicated that bacterioplankton with completely aerobic denitrification ability were lacking in the water samples collected from Lake Taihu. We suggested that adding completely aerobic denitrification bacteria （to denitrify NO2^--N to N2） would improve the water quality. PCR-DGGE and sequencing results showed that more than 1/3 of the bacterial species were associated with the removal of nitrogen, and Acidovorax temperans was the dominant one. PCR-DGGE, variation of nitrogen, removal efficiencies of chlorophyll-a and canonical correspondence analysis indicated that the bacterioplankton significantly influenced the physiological and biochemical changes of cyanobacteria. Additionally, the unweighted pair-group method with arithmetic means revealed there was no obvious harm to the microecosystem from aeration. The present study demonstrated that bacterioplankton can play crucial roles in aerated ecosystems, which could control the impact of cyanobacterial blooms in eutrophicated fresh water systems. 展开更多
关键词 cyanobacterial blooms PCR-DGGE bacterioplankton community aerated ecosystem
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FP-STE: A Novel Node Failure Prediction Method Based on Spatio-Temporal Feature Extraction in Data Centers 认领 引用 被引量：3
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作者 Yang Yang Jing Dong +2 位作者 Chao Fang Ping Xie Na An 《Computer Modeling in Engineering & Sciences》 SCIE EI 2020年第6期1015-1031,共17页
The development of cloud computing and virtualization technology has brought great challenges to the reliability of data center services.Data centers typically contain a large number of compute and storage nodes which... The development of cloud computing and virtualization technology has brought great challenges to the reliability of data center services.Data centers typically contain a large number of compute and storage nodes which may fail and affect the quality of service.Failure prediction is an important means of ensuring service availability.Predicting node failure in cloud-based data centers is challenging because the failure symptoms reflected have complex characteristics,and the distribution imbalance between the failure sample and the normal sample is widespread,resulting in inaccurate failure prediction.Targeting these challenges,this paper proposes a novel failure prediction method FP-STE(Failure Prediction based on Spatio-temporal Feature Extraction).Firstly,an improved recurrent neural network HW-GRU(Improved GRU based on HighWay network)and a convolutional neural network CNN are used to extract the temporal features and spatial features of multivariate data respectively to increase the discrimination of different types of failure symptoms which improves the accuracy of prediction.Then the intermediate results of the two models are added as features into SCSXGBoost to predict the possibility and the precise type of node failure in the future.SCS-XGBoost is an ensemble learning model that is improved by the integrated strategy of oversampling and cost-sensitive learning.Experimental results based on real data sets confirm the effectiveness and superiority of FP-STE. 展开更多
关键词 Failure prediction data center features extraction XGBoost service availability
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Displacement of the retina after idiopathic macular hole surgery with different internal limiting membrane peeling patterns 认领 引用 被引量：3
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作者 Jin Liu Zi-Zhong Hu +6 位作者 Xin-Hua Zheng Yuan-Long Li Jun-Long Huang Er-Bing Cao Song-Tao Yuan Ping Xie Qing-Huai Liu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2021年第9期1408-1412,共5页
AIM:To explore retinal displacement after surgical treatment for idiopathic macular hole(IMH)with different internal limiting membrane(ILM)peeling patterns.METHODS:Totally 22 eyes from 20 patients with IMH were random... AIM:To explore retinal displacement after surgical treatment for idiopathic macular hole(IMH)with different internal limiting membrane(ILM)peeling patterns.METHODS:Totally 22 eyes from 20 patients with IMH were randomly allocated into two groups,N-T group(11 eyes)and T-N group(11 eyes).For patients in N-T group,ILM was peeled off from nasal to temporal retina.For patients in T-N group,ILM was peeled off from temporal to nasal retina.Preoperative,postoperative 1,3,and 6 mo,autofluorescence fundus images were collected for manual measurement of distances of fixed nasal(N),temporal(T),superior(S),and inferior(I)retinal points(bifurcation or crossing of retinal vessels)around the macula to the optic disc(OD).These were respectively defined as N-OD,T-OD,S-OD,and I-OD.The retinal displacement,macular holeclosure rate,and best corrected visual acuity(BCVA)were compared between the two groups after surgery.RESULTS:At postoperative 1,3,and 6 mo,the macula slipped toward the OD,manifested by the decreased T-OD,N-OD,S-OD,and I-OD(P<0.05).No significant difference was found in the T-OD,N-OD,S-OD,and I-OD between N-T group and T-N group.IMH closure rate was 100%both in N-T group and T-N group.There was no significant difference in BCVA between two groups(P<0.05).CONCLUSION:The macula slips toward the OD after successful macular hole surgery.The two different ILM peeling pattern show similar visual outcome and retinal displacement,which means ILM peeling directions are not the influencing factor of postoperative retinal displacement. 展开更多
关键词 retinal displacement idiopathic macular hole internal limiting membrane peeling vitrectomy
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Two Ultraviolet Radiation Datasets that Cover China 认领 引用 被引量：12
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作者 Hui LIU Bo HU +42 位作者 Yuesi WANG Guangren LIU Liqin TANG Dongsheng JI Yongfei BAI Weikai BAO Xin CHEN Yunming CHEN Weixin DING Xiaozeng HAN Fei HE Hui HUANG Zhenying HUANG Xinrong LI Yan LI Wenzhao LIU Luxiang LIN Zhu OUYANG Boqiang QIN Weijun SHEN Yanjun SHEN Hongxin SU Changchun SONG Bo SUN Song SUN Anzhi WANG Genxu WANG Huimin WANG Silong WANG Youshao WANG Wenxue WEI Ping XIE Zongqiang XIE Xiaoyuan YAN Fanjiang ZENG Fawei ZHANG Yangjian ZHANG Yiping ZHANG Chengyi ZHAO Wenzhi ZHAO Xueyong ZHAO Guoyi ZHOU Bo ZHU 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2017年第7期805-815,共11页
Ultraviolet（UV） radiation has significant effects on ecosystems, environments, and human health, as well as atmospheric processes and climate change. Two ultraviolet radiation datasets are described in this paper. O... Ultraviolet（UV） radiation has significant effects on ecosystems, environments, and human health, as well as atmospheric processes and climate change. Two ultraviolet radiation datasets are described in this paper. One contains hourly observations of UV radiation measured at 40 Chinese Ecosystem Research Network stations from 2005 to 2015. CUV3 broadband radiometers were used to observe the UV radiation, with an accuracy of 5%, which meets the World Meteorology Organization＇s measurement standards. The extremum method was used to control the quality of the measured datasets. The other dataset contains daily cumulative UV radiation estimates that were calculated using an all-sky estimation model combined with a hybrid model. The reconstructed daily UV radiation data span from 1961 to 2014. The mean absolute bias error and root-mean-square error are smaller than 30% at most stations, and most of the mean bias error values are negative, which indicates underestimation of the UV radiation intensity. These datasets can improve our basic knowledge of the spatial and temporal variations in UV radiation. Additionally, these datasets can be used in studies of potential ozone formation and atmospheric oxidation, as well as simulations of ecological processes. 展开更多
关键词 reconstructed ozone absolute ultraviolet estimates environments climatic cumulative sunshine meteorological
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Hepatic Histopathological Characteristics and Antioxidant Response of Phytoplanktivorous Silver Carp Intraperitoneally Injected with Extracted Microcystins 认领 引用 被引量：4
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作者 LI LI PING XIE 《Biomedical and Environmental Sciences》 SCIE CAS 2009年第4期297-302,共6页
Objective To investigate the hispathological characteristics and antioxidant responses in liver of silver carp after intraperitoneal administration of microcystins(MCs)for further understanding hepatic intoxication an... Objective To investigate the hispathological characteristics and antioxidant responses in liver of silver carp after intraperitoneal administration of microcystins(MCs)for further understanding hepatic intoxication and antioxidation mechanism in fish.Methods Phytoplanktivorous silver carp was injected intraperitoneally(i.p.)with extracted hepatotoxic microcystins(mainly MC-RR and-LR)at a dose of 1000μg MC-LReq./kg body weight,and liver histopathological changes and antioxidant responses were studied at 1,3,12,24,and 48 h,respectively,after injection.Results The damage to liver structure and the activities of hepatic antioxidant enzymes including catalase(CAT),superoxide dismutase(SOD),and glutathione peroxide(GPX)were increased in a time-dependent manner.Conclusion In terms of clinical and histological signs of intoxication and LD50(i.p.)dose of MC-LR,silver carp appears rather resistant to MCs exposure than other fishes.Also the significantly increased SOD activity in the liver of silver carp suggests a higher degree of response to MCs exposure than CAT and GPX. 展开更多
关键词 Antioxidant response Histopathological characteristics Liver Microcystins Fish
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A Facile Synthesis of Squamosamide Cyclic Analogs 认领 引用 被引量：3
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作者 Xiao Zhen JIAO Ping XIE Xiao Tian LIANG 《Chinese Chemical Letters》 SCIE CAS 2003年第7期667-669,共3页
A series of novel cyclic derivatives have been synthesized by utilizing a nickel powder mediated radical cyclization as the key step. The structures of the new compounds were confirmed by 1H-NMR and MS.
关键词 Squamosamide radical cyclization synthesis.
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The relationship between light intensity and nutrient uptake kinetics in six freshwater diatoms 认领 引用 被引量：4
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作者 Pengling Shi Hong Shen +2 位作者 Wenjing Wang Wenjie Chen Ping Xie 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2015年第8期28-36,共9页
In order to find effective measures to control diatom blooms, a better understanding of the physiological characteristics of nutrient uptake in diatoms is needed. A study of P and Si-uptake kinetics for diatom species... In order to find effective measures to control diatom blooms, a better understanding of the physiological characteristics of nutrient uptake in diatoms is needed. A study of P and Si-uptake kinetics for diatom species from two light regimes was conducted at low（LL）,moderate（ML） and high light intensities（HL）（2, 25 and 80 μmol photons/（m2·sec））,respectively. The results showed that P uptake of diatoms was heavily influenced by historic light regimes. P affinity changed with growth and photosynthetic activity. The lowest half saturation constant for P uptake（Km（P）） was under HL for high-light adapted diatoms while the lowest half-saturation constant for low-light adapted diatoms was observed under LL. The Si half-saturation constant（Km（Si）） increased with increasing light intensities for pennate diatoms but decreased for centric diatoms. Diatom volumes were correlated with the maximum Si uptake rates（Vm（Si）） at HL and K m（Si）at ML and HL for six diatom species. Our results imply that when we assess the development of diatom blooms we should consider light intensity and cell volume in addition to ambient Si or P concentration. The relationship between light intensity and P-uptake suggests that we can find suitable methods to control diatom blooms on the basis of reducing phytoplankton activity of P-uptake and photosynthesis simultaneously. 展开更多
关键词 Diatom Light intensity Michaelis–Menten equation Phosphorus Silicon Uptake kinetics
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Microcystis aeruginosa/Pseudomonas pseudoalcaligenes interaction effects on off-flavors in algae/bacteria co-culture system under different temperatures 认领 引用 被引量：4
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作者 Xi Yang Ping Xie +6 位作者 Yunzhen Yu Hong Shen Xuwei Deng Zhimei Ma Peili Wang Min Tao Yuan Niu 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2015年第5期38-43,共6页
We conducted an experiment to study the interaction effects of Microcystis aeruginosa and Pseudomonas pseudoalcaligenes on off-flavors in an algae/bacteria co-culture system at three temperatures（24, 28 and 32℃）. G... We conducted an experiment to study the interaction effects of Microcystis aeruginosa and Pseudomonas pseudoalcaligenes on off-flavors in an algae/bacteria co-culture system at three temperatures（24, 28 and 32℃）. Gas chromatography-mass spectrometry was applied to measure off-flavor compounds dimethyl sulfide（DMS）, dimethyl trisulfide（DMTS）,2-methylisoborneol, geosmin（GEO） and β-cyclocitral. During the lag phase of co-cultured M. aeruginosa（first 15 days）, P. pseudoalcaligenes significantly increased the production of DMS, DMTS and β-cyclocitral at all three temperatures. In the exponential phase of co-cultured M. aeruginosa（after 15 days）, M. aeruginosa became the main factor on off-flavors in the co-culture system, and β-cyclocitral turned to the highest off-flavor compound. These results also indicated that DMS, DMTS and β-cyclocitral were the main off-flavor compounds in our M. aeruginosa/P. pseudoalcaligenes co-culture system. Univariate analysis was applied to investigate the effects of M. aeruginosa and P. pseudoalcaligenes on the production of off-flavors. The results demonstrated that both M. aeruginosa and P. pseudoalcaligenes could increase the production of DMS and DMTS, while β-cyclocitral was mainly determined by M. aeruginosa. Our results also provide some insights into understanding the relationship between cyanobacteria and heterotrophic bacteria. 展开更多
关键词 Co-culture system Microcystis aeruginosa Off-flavors Pseudomonas pseudoalcaligenes
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H-bonding template-directed synthesis of a complete m-PDA-bridged ladder polyhydrosiloxane (OLPHS) 认领 引用 被引量：1
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作者 Kui Lin Deng Tao Yi Zhang +2 位作者 Xiao Jing Zhang Ping Xie Rong Ben Zhang 《Chinese Chemical Letters》 SCIE CAS 2007年第7期779-782,共4页
A highly ordered m-phenylenediimino-bridged ladder polyhydrosiloxane （abbr. OLPHS） with Mn = 1.24 × 10^4 was synthesized stoichiometric hydrolysis and dehydrochlorination condensation reaction between Si-Cl and... A highly ordered m-phenylenediimino-bridged ladder polyhydrosiloxane （abbr. OLPHS） with Mn = 1.24 × 10^4 was synthesized stoichiometric hydrolysis and dehydrochlorination condensation reaction between Si-Cl and Si-OH bonds. The complete ladder structure of OLPHS has been confirmed by the following three data. Two characteristic Bragg＇s peaks representing the ladder width （w = 0.94 nm） and ladder thickness （t = 0.42 nm） were observed in XRD analysis, which are consistent with those calculated by molecular simulation. The very sharp absorption with a small half-peak width （w1/2 = 0.5 ppm） for [（-HN）HSiO2/2]n moiety of OLPHS in ^29Si NMR spectrum indicated presence of the complete ladder structure. As collateral evidence, a higher glass transition temperature （Tg = 105 ℃） is also recorded in the DSC measurement, implying the high stiffness of ladder chain of OLPHS. 展开更多
关键词 H-bonding template Ladder polysiloxane Self-assembly Stepwise coupling/polymerization
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Extracting intraocular foreign body at or near the ciliary body with scleral indentation in direct visualization 认领 引用 被引量：1
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作者 Jiang-Dong Ji Yu-Zhi Ding +3 位作者 Zi-Zhong Hu Ping Xie Song-Tao Yuan Qing-Huai Liu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2018年第3期533-535,共3页
Dear Editor,I＇m Dr. Qing-Huai Liu from Department of Ophthalmology, the First Affiliated Hospital of Nanjing Medical University,Nanjing, China. I write to present four cases diagnosed with the intraocular foreign bo... Dear Editor,I＇m Dr. Qing-Huai Liu from Department of Ophthalmology, the First Affiliated Hospital of Nanjing Medical University,Nanjing, China. I write to present four cases diagnosed with the intraocular foreign body （IOFB） at or near the ciliary body and to evaluate the effect of extracting IOFB with scleral indentation in direct visualization. 展开更多
关键词 Extracting intraocular foreign body at or near the ciliary body scleral indentation direct visualization
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