Hatchery release is a common and effective practice for protecting and restoring wild resources,and the success of the practice is commonly assessed using mark-recapture technology.We investigated the use of different...Hatchery release is a common and effective practice for protecting and restoring wild resources,and the success of the practice is commonly assessed using mark-recapture technology.We investigated the use of different dimensional X-ray imaging techniques for the strontium(Sr)marking of fish fin rays for stocking.Megalobrama amblycephala juveniles were marked by culturing specimens in 800-mg/L SrCl2·6 H2O solution,the cross-sections of dorsal fin rays were subsequently obtained,and the concentrations Sr was analyzed by 2 D imaging using an electron probe X-ray microanalyzer.Our preliminary findings indicate that the immersion marking method is effective for the Sr marking of fin rays in experimental fish.Moreover,we generated a bird’s-eye-view 3 D mesh image of the Sr concentrations,which can provide a more comprehensive information for fish stocking than that using normal 2 D imaging.展开更多
Background Restocking by introducing hatchery-reared fish into wild habitats aids in the restoration of fishery aquatic ecosystems and reefs to increase the abundance of fish resources,restore the ecological balance o...Background Restocking by introducing hatchery-reared fish into wild habitats aids in the restoration of fishery aquatic ecosystems and reefs to increase the abundance of fish resources,restore the ecological balance of water bodies,and enhance ecosystem functioning.Accurately,rapidly,and effectively evaluating the success of restocking using chemical markers(e.g.,strontium[Sr])remains challenging for fisheries management.Consequently,for nonlethal fish sampling,hard tissues,such as fin rays,have received increasing attention as a target for marking method.However,data on the differences in marking different types of fin rays remain limited.Therefore,we exposed juvenile blunt snout bream individuals(Megalobrama amblycephala)to 0(control group)or 800 mg/L of SrCl2·6H2O(marked group)for 5 days and transferred them into normal aerated water for post-immersion culture.We sampled their pectoral,dorsal,ventral,anal,and caudal fin rays.The Sr marks among the fin types were sampled at 0 and 20 days post-immersion and evaluated using an electron probe micro-analyzer(EPMA)for the five-day Sr/Ca ratios,along with line transect and Sr mapping analyses.Results Sr marking signatures were observed in all fin types in the marked group,with a success rate of up to 100%.Although marking efficiency varied among the different fin ray types,the highest Sr/Ca ratios were most often detected in the dorsal fin.Cross-sectional Sr concentration maps of all fin rays sampled showed high-Sr domains in the marked group;in contrast,the entire cross-sections of the control group displayed low Sr contents,indicating successful marking efficiency.Conclusions Fin ray Sr marking is a successful method for juvenile M.amblycephala,with the advantages of nonlethality and negligible sampling injuries,facilitating the rapid and effective evaluation of Sr marking in restocking M.amblycephala.展开更多
The aquatic fauna of large river systems have been the cornerstones of multiple civilizations throughout human history.Today,they remain critically important as primary resources for humans as well as indicators of ge...The aquatic fauna of large river systems have been the cornerstones of multiple civilizations throughout human history.Today,they remain critically important as primary resources for humans as well as indicators of general ecosystem structure and function.Unfortunately,nearly all large-river systems globally are at risk from over-exploitation,pollution,large-scale development,navigation,dredging,climate change,and other threats.For instance,human stressors(such as dams,navigation,agriculture,fshing)and fooding and droughts have afected aquatic biological resources in both the Mississippi and Yangzte River basins(Chen et al.2016).展开更多
基金Supported by the National Key Research and Development Program of China(No.2022 YFF 0608203)the Graduate Student Scientific Research Innovation Projects in Jiangsu Province(No.KYCX 22-0706)。
摘要Hatchery release is a common and effective practice for protecting and restoring wild resources,and the success of the practice is commonly assessed using mark-recapture technology.We investigated the use of different dimensional X-ray imaging techniques for the strontium(Sr)marking of fish fin rays for stocking.Megalobrama amblycephala juveniles were marked by culturing specimens in 800-mg/L SrCl2·6 H2O solution,the cross-sections of dorsal fin rays were subsequently obtained,and the concentrations Sr was analyzed by 2 D imaging using an electron probe X-ray microanalyzer.Our preliminary findings indicate that the immersion marking method is effective for the Sr marking of fin rays in experimental fish.Moreover,we generated a bird’s-eye-view 3 D mesh image of the Sr concentrations,which can provide a more comprehensive information for fish stocking than that using normal 2 D imaging.
基金financed by project grant 2022YFF0608203 of the National Key Research and Development Programproject grant KYCX22_0706 of the Postgraduate Research&Practice Innovation Program of Jiangsu Province
摘要Background Restocking by introducing hatchery-reared fish into wild habitats aids in the restoration of fishery aquatic ecosystems and reefs to increase the abundance of fish resources,restore the ecological balance of water bodies,and enhance ecosystem functioning.Accurately,rapidly,and effectively evaluating the success of restocking using chemical markers(e.g.,strontium[Sr])remains challenging for fisheries management.Consequently,for nonlethal fish sampling,hard tissues,such as fin rays,have received increasing attention as a target for marking method.However,data on the differences in marking different types of fin rays remain limited.Therefore,we exposed juvenile blunt snout bream individuals(Megalobrama amblycephala)to 0(control group)or 800 mg/L of SrCl2·6H2O(marked group)for 5 days and transferred them into normal aerated water for post-immersion culture.We sampled their pectoral,dorsal,ventral,anal,and caudal fin rays.The Sr marks among the fin types were sampled at 0 and 20 days post-immersion and evaluated using an electron probe micro-analyzer(EPMA)for the five-day Sr/Ca ratios,along with line transect and Sr mapping analyses.Results Sr marking signatures were observed in all fin types in the marked group,with a success rate of up to 100%.Although marking efficiency varied among the different fin ray types,the highest Sr/Ca ratios were most often detected in the dorsal fin.Cross-sectional Sr concentration maps of all fin rays sampled showed high-Sr domains in the marked group;in contrast,the entire cross-sections of the control group displayed low Sr contents,indicating successful marking efficiency.Conclusions Fin ray Sr marking is a successful method for juvenile M.amblycephala,with the advantages of nonlethality and negligible sampling injuries,facilitating the rapid and effective evaluation of Sr marking in restocking M.amblycephala.
基金supported by National Key Research and Development Program of China(2023YFC3209002,2019YFD0901203)Chinese Academy of Sciences(QYZDB-SSW-SMC041,ZDRW-ZS-2017-3-2)
摘要The aquatic fauna of large river systems have been the cornerstones of multiple civilizations throughout human history.Today,they remain critically important as primary resources for humans as well as indicators of general ecosystem structure and function.Unfortunately,nearly all large-river systems globally are at risk from over-exploitation,pollution,large-scale development,navigation,dredging,climate change,and other threats.For instance,human stressors(such as dams,navigation,agriculture,fshing)and fooding and droughts have afected aquatic biological resources in both the Mississippi and Yangzte River basins(Chen et al.2016).