To pursue wide applications of Mg-Li alloys in aerospace field,bringing in reinforcement phases reasonably and manufacturing efficiently have become paramount.Herein,a novel rotation friction extrusion process was pro...To pursue wide applications of Mg-Li alloys in aerospace field,bringing in reinforcement phases reasonably and manufacturing efficiently have become paramount.Herein,a novel rotation friction extrusion process was proposed to fabricate Fe Co Ni Cr Cu high entropy alloy reinforced Mg-Li matrix composites,which were enhanced by newly formed nano-sized Al Cu Mg phase particles.The grain size of Mg-Li matrix composites was 4.19μm.Meanwhile,a 69.03 nm thickness amorphous layer was obtained,which was composed of amorphous and nanocrystalline structure.The maximum tensile strength reached 201.3 MPa,which was improved by 43.9%compared with LAZ933 Mg-Li alloy.It can be attributed to fine grain strengthening and dispersion strengthening,which resulted from dynamic recrystallization in Mg-Li alloy and Cu segregation from the interior of high entropy alloy.This work shows a novel processing and original reinforcement phase,providing a peculiar way to fabricate Mg-Li matrix composites.展开更多
Friction stir lap welding(FSLW)was adopted to join successfully dissimilar AZ31B Mg alloy and TC4 Ti alloy with Sn foil addition of 30μm thickness.Interfacial microstructure,tensile shear performances and bonding mec...Friction stir lap welding(FSLW)was adopted to join successfully dissimilar AZ31B Mg alloy and TC4 Ti alloy with Sn foil addition of 30μm thickness.Interfacial microstructure,tensile shear performances and bonding mechanism of the joints obtained using three different rotation speeds were studied.High-performance FSLW Mg/Ti dissimilar joints with maximum tensile shear strength of 593.3 N/mm were produced at 1180 r/min,and which was mainly attributed to ultrastrong reaction interlayer consisting of 125.9 nm thick(Mg2Sn+Mg)transition layer and discontinuous(Ti6Sn5+Ti3Al)IMCs layer with 6.58 nm thickness at the interface.The formation of the reaction interlayer was beneficial for high interfacial strength,resulting in significantly improving the joint strength.The fracture of all FSLW joints located on AZ31B Mg stirred zone adjacent to(Mg2Sn+Mg)transition layer or along the crack propagation direction of the AZ31B/TC4 interface with different fracture mechanisms,and which could be consistent with interfacial microstructure.展开更多
The corrosion degradation of organic coatings in tropical marine atmospheric environments results in substantial economic losses across various industries.The complexity of a dynamic environment,combined with high cos...The corrosion degradation of organic coatings in tropical marine atmospheric environments results in substantial economic losses across various industries.The complexity of a dynamic environment,combined with high costs,extended experimental periods,and limited data,places a limit on the comprehension of this process.This study addresses this challenge by investigating the corrosion de-gradation of damaged organic coatings in a tropical marine environment using an atmospheric corrosion monitoring sensor and a random forest(RF)model.For damage simulation,a polyurethane coating applied to a Fe/graphite corrosion sensor was intentionally scratched and exposed to the marine atmosphere for over one year.Pearson correlation analysis was performed for the collection and filtering of en-vironmental and corrosion current data.According to the RF model,the following specific conditions contributed to accelerated degrada-tion:relative humidity(RH)above 80%and temperatures below 22.5℃,with the risk increasing significantly when RH exceeded 90%.High RH and temperature exhibited a cumulative effect on coating degradation.A high risk of corrosion occurred in the nighttime.The RF model was also used to predict the coating degradation process using environmental data as input parameters,with the accuracy show-ing improvement when the duration of influential environmental ranges was considered.展开更多
Biodegradable magnesium alloys have excellent properties with respect to biodegradability, biocompatibility, and biomechanics, which may indicate a possibility of its application in intestinal stents. Investigation of...Biodegradable magnesium alloys have excellent properties with respect to biodegradability, biocompatibility, and biomechanics, which may indicate a possibility of its application in intestinal stents. Investigation of Mg-Zn-Y-Nd alloy’s application in intestinal stents has been performed. This study aims to investigate the degradation behavior of Mg-Zn-Y-Nd alloy intestinal stents coated with poly(L-lactide)/paclitaxel in the intestinal environment and its biocompatibility with intestinal tissue. In this paper, Mg-Zn-Y-Nd alloy’s corrosion properties were evaluated by the immersion test in human feces, SEM and XRD, and animal tests. In vitro results showed that when the Mg-Zn-Y-Nd alloy was immersed in human feces for two weeks, its corrosion resistance could be improved by micro arc oxidation(MAO) and poly-l-lactide(PLLA) dual coating. Additionally, this result was also confirmed in vivo experiments by rabbit model. And animal tests also demonstrated that the Mg-Zn-Y-Nd alloy with MAO/PLLA/paclitaxel dual coating drug-eluting stents could inhibit the proliferation of local intestinal tissue around the stents. However, in vivo studies illustrated that the intestinal stents gradually degraded in rabbit model within 12 days.Considering the degradation rate of the stent was faster than expected in rabbits, the support performance of the scaffold requires further improvement.展开更多
Foam drainage is theflow of liquid through the interstitial spaces between bubbles driven by capillarity and grav-ity and resisted by viscous damping.The so-called foam drainage gas recovery technology is a technique ...Foam drainage is theflow of liquid through the interstitial spaces between bubbles driven by capillarity and grav-ity and resisted by viscous damping.The so-called foam drainage gas recovery technology is a technique tradi-tionally used to mitigate the serious bottom-hole liquid loading in the middle and late stages of gas well production.In this context,determining the optimal concentration of the bubble drainage agent is generally cru-cial for the proper application of this method.In this study,a combination of indoor experiments and theoretical analysis have been used to determine the pressure drop related to the foam-carrying capacity in a representative gasfield.Dynamic and static experiments were designed with a bubble drainage agent concentration varying in the range 0.3%–0.6%.Using thefield formation water data,the optimal soaking agent concentration was obtained and pressure drop test experiments on the foam carrying capacity were conducted accordingly.These tests have revealed that the optimal foam displacement agent concentration is 0.5%,and the foam quality at the optimum concentration is between 0.78–0.98.A theoretical method for calculating the pressure drop at the optimum soak-away concentration based on experimental data has also been introduced.The error of the proposed method is within 15%compared to the experimental measured value,demonstrating that it is highly accurate and simple.展开更多
This paper presents a multi-sensor ensemble classifier (MSEC) for physical activity (PA) pattern recognition of human subjects. The MSEC, developed for a wearable multi-sensor integrate measurement system (IMS),combin...This paper presents a multi-sensor ensemble classifier (MSEC) for physical activity (PA) pattern recognition of human subjects. The MSEC, developed for a wearable multi-sensor integrate measurement system (IMS),combines multiple classifiers based on different sensor feature sets to improve the accuracy of PA type identification.Experimental evaluation of 56 subjects has shown that the MSECis more effectivein assessing activities of varying intensitiesthan the traditional homogeneous classifiers. It is able to correctly recognize 6 PA types with an accuracy of 93.50%, which is 7% higher than the non-ensemble support vector machine method. Furthermore, the MSECis effective in reducing the subject-to-subject variabilityin activity recognition.展开更多
It is very important to adopt standardized production technology in the process of maize cultivation,which is conducive to giving full play to the high-yield,high-quality and multi-resistant characteristics of Jinhai ...It is very important to adopt standardized production technology in the process of maize cultivation,which is conducive to giving full play to the high-yield,high-quality and multi-resistant characteristics of Jinhai 5.This paper standardized the cultivation technology of Jinhai 5 from the aspects of maize seed quality,fertilization before sowing,sowing,seedling management,mid-period management,later management,disease and pest control,and harvesting,so as to provide reference for the standardized production of maize Jinhai 5.展开更多
Randomized controlled trials(RCTs)provide optimal evidence of the effectiveness and safety of a new drug,a new medical device,or a new therapeutic strategy with the necessary scientific design[1].Traditional electroni...Randomized controlled trials(RCTs)provide optimal evidence of the effectiveness and safety of a new drug,a new medical device,or a new therapeutic strategy with the necessary scientific design[1].Traditional electronic data collection(EDC)systems for clinical trials primarily focus on data entry,validation,and compliance monitoring.However,their reliance on centralized architectures introduces vulnerabilities in data security and integrity。展开更多
This paper focuses on the problem of active object detection(AOD).AOD is important for service robots to complete tasks in the family environment,and leads robots to approach the target ob ject by taking appropriate m...This paper focuses on the problem of active object detection(AOD).AOD is important for service robots to complete tasks in the family environment,and leads robots to approach the target ob ject by taking appropriate moving actions.Most of the current AOD methods are based on reinforcement learning with low training efficiency and testing accuracy.Therefore,an AOD model based on a deep Q-learning network(DQN)with a novel training algorithm is proposed in this paper.The DQN model is designed to fit the Q-values of various actions,and includes state space,feature extraction,and a multilayer perceptron.In contrast to existing research,a novel training algorithm based on memory is designed for the proposed DQN model to improve training efficiency and testing accuracy.In addition,a method of generating the end state is presented to judge when to stop the AOD task during the training process.Sufficient comparison experiments and ablation studies are performed based on an AOD dataset,proving that the presented method has better performance than the comparable methods and that the proposed training algorithm is more effective than the raw training algorithm.展开更多
The rheumatoid arthritis(RA)microenvironment is often followed by a vicious circle of high inflammation,endogenous gas levels imbalance,and poor treatment.To break the circle,we develop a dual-gas-mediated injectable ...The rheumatoid arthritis(RA)microenvironment is often followed by a vicious circle of high inflammation,endogenous gas levels imbalance,and poor treatment.To break the circle,we develop a dual-gas-mediated injectable hydrogel for modulating the immune microenvironment of RA and simultaneously releasing therapeutic drugs.The hydrogel(DNRS gel)could be broken down on-demand by consuming excessive nitric oxide(NO)and releasing therapeutic hydrogen sulfide(H2S),resulting in endogenous gas restoration,inflammation alleviation,and macrophage polarization to M2 type.Additionally,the hydrogel could suppress osteoclastogenesis and enhance osteogenesis.Furthermore,the intra-articularly injected hydrogel with methotrexate(MTX/DNRS gel)significantly alleviated inflammation and clinical symptoms and promoted the repair of bone erosion in the collagen-induced arthritis rat model.As a result,in vivo results demonstrated that MTX/DNRS gel restored the microenvironment and improved the therapeutic effect of MTX.This study provides a novel understanding of developing versatile smart delivery platforms for RA treatment.展开更多
Assay for transposase-accessible chromatin with high-throughput sequencing(ATAC-seq) is a technique widely used to investigate genome-wide chromatin accessibility. The recently published Omni-ATAC-seq protocol substan...Assay for transposase-accessible chromatin with high-throughput sequencing(ATAC-seq) is a technique widely used to investigate genome-wide chromatin accessibility. The recently published Omni-ATAC-seq protocol substantially improves the signaloise ratio and reduces the input cell number. High-quality data are critical to ensure accurate analysis.Several tools have been developed for assessing sequencing quality and insertion size distribution for ATAC-seq data;however, key quality control(QC) metrics have not yet been established to accurately determine the quality of ATAC-seq data. Here, we optimized the analysis strategy for ATAC-seq and defined a series of QC metrics for ATAC-seq data,including reads under peak ratio(RUPr), background(BG), promoter enrichment(Pro En), subsampling enrichment(Sub En), and other measurements. We incorporated these QC tests into our recently developed ATAC-seq Integrative Analysis Package(AIAP) to provide a complete ATAC-seq analysis system, including quality assurance, improved peak calling, and downstream differential analysis. We demonstrated a significant improvement of sensitivity(20%–60%) in both peak calling and differential analysis by processing paired-end ATAC-seq datasets using AIAP. AIAP is compiled into Docker/Singularity, and it can be executed by one command line to generate a comprehensive QC report. We used ENCODE ATAC-seq data to benchmark and generate QC recommendations, and developed q ATACViewer for the userfriendly interaction with the QC report. The software, source code, and documentation of AIAP are freely available at http://gffzz188fe103f8f1460as6ucbxw9wcfuf6bcw.ffgz.tsg.suse.edu.cn/Zhang-lab/ATAC-seq_QC_analysis.展开更多
The realization of pseudomagnetic fields for lightwaves has attained great attention in the field of nanophotonics.Like real magnetic fields,Landau quantization could be induced by pseudomagnetic fields in the straine...The realization of pseudomagnetic fields for lightwaves has attained great attention in the field of nanophotonics.Like real magnetic fields,Landau quantization could be induced by pseudomagnetic fields in the strainengineered graphene.We demonstrated that pseudomagnetic fields can also be introduced to photonic crystals by exerting a linear parabolic deformation onto the honeycomb lattices,giving rise to degenerate energy states and flat plateaus in the photonic band structures.We successfully inspire the photonic snake modes corresponding to the helical state in the synthetic magnetic heterostructure by adopting a microdisk for the unidirectional coupling.By integrating heat electrodes,we can further electrically manipulate the photonic density of states for the uniaxially strained photonic crystal.This offers an unprecedented opportunity to obtain on-chip robust optical transports under the electrical tunable pseudomagnetic fields,opening the possibility to design Si-based functional topological photonic devices.展开更多
基金co-supported by the National Natural Science Foundation of China(Nos.52164045,52465044)the Outstanding Youth Foundation in Jiangxi Province,China(No.20224ACB214012)the Key Project of Jiangxi Provincial Natural Science Foundation,China(No.20252BAC250045)。
摘要To pursue wide applications of Mg-Li alloys in aerospace field,bringing in reinforcement phases reasonably and manufacturing efficiently have become paramount.Herein,a novel rotation friction extrusion process was proposed to fabricate Fe Co Ni Cr Cu high entropy alloy reinforced Mg-Li matrix composites,which were enhanced by newly formed nano-sized Al Cu Mg phase particles.The grain size of Mg-Li matrix composites was 4.19μm.Meanwhile,a 69.03 nm thickness amorphous layer was obtained,which was composed of amorphous and nanocrystalline structure.The maximum tensile strength reached 201.3 MPa,which was improved by 43.9%compared with LAZ933 Mg-Li alloy.It can be attributed to fine grain strengthening and dispersion strengthening,which resulted from dynamic recrystallization in Mg-Li alloy and Cu segregation from the interior of high entropy alloy.This work shows a novel processing and original reinforcement phase,providing a peculiar way to fabricate Mg-Li matrix composites.
基金supported by the National Natural Science Foundation of China(NSFC)(Nos.52005240,52164045)Young Talent Program of Major Disciplines of Academic and Technical Leaders in Jiangxi Province(No.20212BCJ23028)Outstanding Youth Foundation in Jiangxi Province(No.20224ACB214012).
摘要Friction stir lap welding(FSLW)was adopted to join successfully dissimilar AZ31B Mg alloy and TC4 Ti alloy with Sn foil addition of 30μm thickness.Interfacial microstructure,tensile shear performances and bonding mechanism of the joints obtained using three different rotation speeds were studied.High-performance FSLW Mg/Ti dissimilar joints with maximum tensile shear strength of 593.3 N/mm were produced at 1180 r/min,and which was mainly attributed to ultrastrong reaction interlayer consisting of 125.9 nm thick(Mg2Sn+Mg)transition layer and discontinuous(Ti6Sn5+Ti3Al)IMCs layer with 6.58 nm thickness at the interface.The formation of the reaction interlayer was beneficial for high interfacial strength,resulting in significantly improving the joint strength.The fracture of all FSLW joints located on AZ31B Mg stirred zone adjacent to(Mg2Sn+Mg)transition layer or along the crack propagation direction of the AZ31B/TC4 interface with different fracture mechanisms,and which could be consistent with interfacial microstructure.
基金supported by the National Key R&D Program of China(No.2022YFB3808803)the National Natural Science Foundation of China(No.52371049)the National Science and Technology Resources Investigation Program of China(No.2021FY100603).
摘要The corrosion degradation of organic coatings in tropical marine atmospheric environments results in substantial economic losses across various industries.The complexity of a dynamic environment,combined with high costs,extended experimental periods,and limited data,places a limit on the comprehension of this process.This study addresses this challenge by investigating the corrosion de-gradation of damaged organic coatings in a tropical marine environment using an atmospheric corrosion monitoring sensor and a random forest(RF)model.For damage simulation,a polyurethane coating applied to a Fe/graphite corrosion sensor was intentionally scratched and exposed to the marine atmosphere for over one year.Pearson correlation analysis was performed for the collection and filtering of en-vironmental and corrosion current data.According to the RF model,the following specific conditions contributed to accelerated degrada-tion:relative humidity(RH)above 80%and temperatures below 22.5℃,with the risk increasing significantly when RH exceeded 90%.High RH and temperature exhibited a cumulative effect on coating degradation.A high risk of corrosion occurred in the nighttime.The RF model was also used to predict the coating degradation process using environmental data as input parameters,with the accuracy show-ing improvement when the duration of influential environmental ranges was considered.
基金the National Natural Science Foundation of China (No. U04825)the Key Scientific and Technological Projects of Henan Province (No. 2102310012)+2 种基金the Natural Science Foundation of Henan Province (No. 2300410241)the National Key Research and Development Program of China (2018YFC1106703)the Science and Technology Development Projects of Luoyang City (No. 03006A-3)。
摘要Biodegradable magnesium alloys have excellent properties with respect to biodegradability, biocompatibility, and biomechanics, which may indicate a possibility of its application in intestinal stents. Investigation of Mg-Zn-Y-Nd alloy’s application in intestinal stents has been performed. This study aims to investigate the degradation behavior of Mg-Zn-Y-Nd alloy intestinal stents coated with poly(L-lactide)/paclitaxel in the intestinal environment and its biocompatibility with intestinal tissue. In this paper, Mg-Zn-Y-Nd alloy’s corrosion properties were evaluated by the immersion test in human feces, SEM and XRD, and animal tests. In vitro results showed that when the Mg-Zn-Y-Nd alloy was immersed in human feces for two weeks, its corrosion resistance could be improved by micro arc oxidation(MAO) and poly-l-lactide(PLLA) dual coating. Additionally, this result was also confirmed in vivo experiments by rabbit model. And animal tests also demonstrated that the Mg-Zn-Y-Nd alloy with MAO/PLLA/paclitaxel dual coating drug-eluting stents could inhibit the proliferation of local intestinal tissue around the stents. However, in vivo studies illustrated that the intestinal stents gradually degraded in rabbit model within 12 days.Considering the degradation rate of the stent was faster than expected in rabbits, the support performance of the scaffold requires further improvement.
基金support provided by the National Natural Science Foundation of China(No.62173049)the Open Fund of the Key Laboratory of Exploration Technologies for Oil and Gas Resources(Yangtze University),Ministry of Education(Grant K2021-17).
摘要Foam drainage is theflow of liquid through the interstitial spaces between bubbles driven by capillarity and grav-ity and resisted by viscous damping.The so-called foam drainage gas recovery technology is a technique tradi-tionally used to mitigate the serious bottom-hole liquid loading in the middle and late stages of gas well production.In this context,determining the optimal concentration of the bubble drainage agent is generally cru-cial for the proper application of this method.In this study,a combination of indoor experiments and theoretical analysis have been used to determine the pressure drop related to the foam-carrying capacity in a representative gasfield.Dynamic and static experiments were designed with a bubble drainage agent concentration varying in the range 0.3%–0.6%.Using thefield formation water data,the optimal soaking agent concentration was obtained and pressure drop test experiments on the foam carrying capacity were conducted accordingly.These tests have revealed that the optimal foam displacement agent concentration is 0.5%,and the foam quality at the optimum concentration is between 0.78–0.98.A theoretical method for calculating the pressure drop at the optimum soak-away concentration based on experimental data has also been introduced.The error of the proposed method is within 15%compared to the experimental measured value,demonstrating that it is highly accurate and simple.
摘要This paper presents a multi-sensor ensemble classifier (MSEC) for physical activity (PA) pattern recognition of human subjects. The MSEC, developed for a wearable multi-sensor integrate measurement system (IMS),combines multiple classifiers based on different sensor feature sets to improve the accuracy of PA type identification.Experimental evaluation of 56 subjects has shown that the MSECis more effectivein assessing activities of varying intensitiesthan the traditional homogeneous classifiers. It is able to correctly recognize 6 PA types with an accuracy of 93.50%, which is 7% higher than the non-ensemble support vector machine method. Furthermore, the MSECis effective in reducing the subject-to-subject variabilityin activity recognition.
基金Agricultural Major Applied Technology Innovation Project of Shandong Province。
摘要It is very important to adopt standardized production technology in the process of maize cultivation,which is conducive to giving full play to the high-yield,high-quality and multi-resistant characteristics of Jinhai 5.This paper standardized the cultivation technology of Jinhai 5 from the aspects of maize seed quality,fertilization before sowing,sowing,seedling management,mid-period management,later management,disease and pest control,and harvesting,so as to provide reference for the standardized production of maize Jinhai 5.
基金supported by the National Natural Science Foundation of China(92368205 and 82301265)the Guangdong Provincial Natural Science Foundation for Progressive Young Scholars(2023A1515030170)+3 种基金the Science and Technology Planning Projects of Guangdong Province(2018B010109008)the Science and Technology Planning Project of the Guangzhou Municipal Health Commission(2024A031004)the Basic Scientific Research Projects of Sun Yat-sen University(23ykcxqt002)the China Postdoctoral Science Foundation(2023M734047).
摘要Randomized controlled trials(RCTs)provide optimal evidence of the effectiveness and safety of a new drug,a new medical device,or a new therapeutic strategy with the necessary scientific design[1].Traditional electronic data collection(EDC)systems for clinical trials primarily focus on data entry,validation,and compliance monitoring.However,their reliance on centralized architectures introduces vulnerabilities in data security and integrity。
基金supported by the National Natural Science Foundation of China(Nos.U1813215 and 62273203)。
摘要This paper focuses on the problem of active object detection(AOD).AOD is important for service robots to complete tasks in the family environment,and leads robots to approach the target ob ject by taking appropriate moving actions.Most of the current AOD methods are based on reinforcement learning with low training efficiency and testing accuracy.Therefore,an AOD model based on a deep Q-learning network(DQN)with a novel training algorithm is proposed in this paper.The DQN model is designed to fit the Q-values of various actions,and includes state space,feature extraction,and a multilayer perceptron.In contrast to existing research,a novel training algorithm based on memory is designed for the proposed DQN model to improve training efficiency and testing accuracy.In addition,a method of generating the end state is presented to judge when to stop the AOD task during the training process.Sufficient comparison experiments and ablation studies are performed based on an AOD dataset,proving that the presented method has better performance than the comparable methods and that the proposed training algorithm is more effective than the raw training algorithm.
基金financially supported by National Key Research and Development Program of China(2022YFB3804400)National Natural Science Foundation of China(52333011,and 82102225)+1 种基金Venture&Innovation Support Program for Chongqing Overseas Returnees(cx2023104)Natural Science Foundation of Chongqing(cstc2021jcyj-cxttX0002).
摘要The rheumatoid arthritis(RA)microenvironment is often followed by a vicious circle of high inflammation,endogenous gas levels imbalance,and poor treatment.To break the circle,we develop a dual-gas-mediated injectable hydrogel for modulating the immune microenvironment of RA and simultaneously releasing therapeutic drugs.The hydrogel(DNRS gel)could be broken down on-demand by consuming excessive nitric oxide(NO)and releasing therapeutic hydrogen sulfide(H2S),resulting in endogenous gas restoration,inflammation alleviation,and macrophage polarization to M2 type.Additionally,the hydrogel could suppress osteoclastogenesis and enhance osteogenesis.Furthermore,the intra-articularly injected hydrogel with methotrexate(MTX/DNRS gel)significantly alleviated inflammation and clinical symptoms and promoted the repair of bone erosion in the collagen-induced arthritis rat model.As a result,in vivo results demonstrated that MTX/DNRS gel restored the microenvironment and improved the therapeutic effect of MTX.This study provides a novel understanding of developing versatile smart delivery platforms for RA treatment.
基金supported by the National Institutes of Health(Grant Nos.U24ES026699,U01HG009391,and R25DA027995)the Goldman Sachs Philanthropy Fund(Emerson Collective)Chan Zuckerberg Initiative,United States。
摘要Assay for transposase-accessible chromatin with high-throughput sequencing(ATAC-seq) is a technique widely used to investigate genome-wide chromatin accessibility. The recently published Omni-ATAC-seq protocol substantially improves the signaloise ratio and reduces the input cell number. High-quality data are critical to ensure accurate analysis.Several tools have been developed for assessing sequencing quality and insertion size distribution for ATAC-seq data;however, key quality control(QC) metrics have not yet been established to accurately determine the quality of ATAC-seq data. Here, we optimized the analysis strategy for ATAC-seq and defined a series of QC metrics for ATAC-seq data,including reads under peak ratio(RUPr), background(BG), promoter enrichment(Pro En), subsampling enrichment(Sub En), and other measurements. We incorporated these QC tests into our recently developed ATAC-seq Integrative Analysis Package(AIAP) to provide a complete ATAC-seq analysis system, including quality assurance, improved peak calling, and downstream differential analysis. We demonstrated a significant improvement of sensitivity(20%–60%) in both peak calling and differential analysis by processing paired-end ATAC-seq datasets using AIAP. AIAP is compiled into Docker/Singularity, and it can be executed by one command line to generate a comprehensive QC report. We used ENCODE ATAC-seq data to benchmark and generate QC recommendations, and developed q ATACViewer for the userfriendly interaction with the QC report. The software, source code, and documentation of AIAP are freely available at http://gffzz188fe103f8f1460as6ucbxw9wcfuf6bcw.ffgz.tsg.suse.edu.cn/Zhang-lab/ATAC-seq_QC_analysis.
基金National Natural Science Foundation of China (62105158,62075038,12104233,12004189)Natural Science Foundation of Jiangsu Province (BK20210645)+2 种基金Natural Science Research of Jiangsu Higher Education Institutions of China (21KJB140012)Innovation and Entrepreneurship Program of Jiangsu Province (JSSCBS20210467)Key Research and Development Program of Jiangsu Province (BE2021667)。
摘要The realization of pseudomagnetic fields for lightwaves has attained great attention in the field of nanophotonics.Like real magnetic fields,Landau quantization could be induced by pseudomagnetic fields in the strainengineered graphene.We demonstrated that pseudomagnetic fields can also be introduced to photonic crystals by exerting a linear parabolic deformation onto the honeycomb lattices,giving rise to degenerate energy states and flat plateaus in the photonic band structures.We successfully inspire the photonic snake modes corresponding to the helical state in the synthetic magnetic heterostructure by adopting a microdisk for the unidirectional coupling.By integrating heat electrodes,we can further electrically manipulate the photonic density of states for the uniaxially strained photonic crystal.This offers an unprecedented opportunity to obtain on-chip robust optical transports under the electrical tunable pseudomagnetic fields,opening the possibility to design Si-based functional topological photonic devices.