Modern land plants comprise two main lineages:tracheophytes and bryophytes.The latter include mosses,liverworts,and hornworts.Bryophytes are second only to angiosperms in diversity,with approximately 22,000 species(Sh...Modern land plants comprise two main lineages:tracheophytes and bryophytes.The latter include mosses,liverworts,and hornworts.Bryophytes are second only to angiosperms in diversity,with approximately 22,000 species(Shaw,2008).They are characterized by a life cycle dominated by haploid gametophytes,with unbranched diploid sporophytes attached to the gametophytes,producing spores for sexual reproduction(Shaw and Renzaglia,2004).Bryophytes have demonstrated great adaptability to modern ecosystems,thriving in environments ranging from deserts to wetlands and from tropical to polar regions(Degola et al.,2022).This adaptability suggests that bryophytes may have evolved an effective genetic toolkit for stress tolerance.Advances in genome sequencing and assembly technologies offer great opportunities to decipher the genetic toolkit and study the evolution of resistance and environmental adaptation in bryophytes.展开更多
Spatially confined microenvironments offer exceptional potential for regulating catalytic activity and selectivity.This study elucidates how the dynamic evolution of carbonaceous species during syngas conversion alter...Spatially confined microenvironments offer exceptional potential for regulating catalytic activity and selectivity.This study elucidates how the dynamic evolution of carbonaceous species during syngas conversion alters the confined environment within MCM-22 cages.We establish a confinement energy,quantified through ethylene adsorption measurements,as a key descriptor correlating with hydrogenation activity at Brönsted acid sites.As carbonaceous deposits expand in size during syngas reaction,they progressively occupy cage volume and reduce the available space,thereby enhancing confinement energy.Such energy gain universally weakens reactant adsorption,simultaneously suppressing hydrogenation activity and promoting olefin selectivity.Collectively,these findings advance fundamental understanding of dynamic confinement effects and provide valuable insights for further designing high-selectivity catalysts for syngas conversion.展开更多
Liverworts,with approximately 7300 species worldwide,exhibit remarkable morphological diversity in terms of growth form,ontogeny,and architecture.Their mitochondrial genome exhibits lower average substitution rates co...Liverworts,with approximately 7300 species worldwide,exhibit remarkable morphological diversity in terms of growth form,ontogeny,and architecture.Their mitochondrial genome exhibits lower average substitution rates compared to their nuclear and plastid genomes,and shows less structural variation,suggesting its suitability for inferring relationships at higher taxonomic levels.In this study,we substantially expanded mitochondrial sampling in liverworts by adding complete mitochondrial gene sets from 97 species across 25 families,thereby increasing family-level coverage to 71%.Among these,we newly assembled 23 complete mitochondrial genomes.Although four species with structural variants were newly identified,the overall architecture of liverwort mitochondrial genomes remains highly conserved,with taxa that diverged over 470 million years ago still having collinearity.Phylogenetic inferences from mitochondrial genome sequences confirmed the monophyly of most suprafamilial taxa,with the exceptions of Porellales,Ptilidiales,and Pelliidae.Herzogianthus(Ptilidiales)was well-supported as a sister group to Jungermanniales sensu lato,rather than forming a monophyletic lineage with Ptilidium(Ptilidiales).This work provides an important resource for future genetic and phylogenetic studies of liverworts.展开更多
Selective electrocatalytic 5-hydroxymethylfurfural(HMF)oxidation reaction(HMFOR)is believed to be highly dependent on the surface adsorption behaviors.Herein,a series of CuAu alloys with various metallic compositions ...Selective electrocatalytic 5-hydroxymethylfurfural(HMF)oxidation reaction(HMFOR)is believed to be highly dependent on the surface adsorption behaviors.Herein,a series of CuAu alloys with various metallic compositions and steered surface adsorption behaviors were prepared by a one-step electrodeposition method.By optimizing the Cu molar ratios,a superior electrocatalytic performance for HMFOR via one-electron dehydrogenation could be observed over Cu0.25Au0.75,reaching a current density of 84.8 mA cm–2at 0.4 V vs.RHE,with 94.5%5-hydroxymethyl-2-furancarboxylic acid(HMFCA)yield,98.6%HMFCA faradaic efficiency(FE)and 94.6%H2FE.Remarkably,with Cu0.25Au0.75as the anode and Pt/C as the cathode,the integrated membrane electrode assembly electrolyzer could operate at a low cell voltage of 0.45 V for simultaneous HMFOR and bipolar H2production,with 94.5%HMFCA FE and~200%H2FE.Experimental investigations and theoretical calculations demonstrate that the alloying induced charge redistribution between Cu and Au could modulate the d-band centers,and then steer the surface adsorption behaviors to balance HMF adsorption and HMFCA desorption at surface,ensuring the active site availability for one-electron dehydrogenation HMFOR.This work presents a facile strategy for designing efficient electrocatalysts for ultralow-potential HMFOR,with fundamental surface adsorption behaviors deepened for biomass electrooxidation.展开更多
Objective:Hyper-progression recurrence(HPR)after hepatectomy is a specific recurrence pattern associated with extremely poor prognosis in patients with hepatocellular carcinoma(HCC).This study was aimed at investigati...Objective:Hyper-progression recurrence(HPR)after hepatectomy is a specific recurrence pattern associated with extremely poor prognosis in patients with hepatocellular carcinoma(HCC).This study was aimed at investigating the probable risk factors and establishing comprehensive models for formulating clinical strategies.Methods:Overall,16,158 patients with HCC from 8 hospitals were screened,among whom 3,125 patients who underwent R0 resection were included,and divided into development(n=2,113)and validation(n=1,012)cohorts.A comprehensive study of HPR predictive models and biological features was conducted.Results:Among the 3,125 enrolled patients,506(16.19%)developed HPR.The influence of HPR on extremely poor prognosis was reflected by recurrence features,adverse effects on systemic and liver function,and limited therapeutic options.Nine variables closely associated with HPR were identified,and incorporated into nomogram and conditional inference tree models,which successfully achieved pre-and post-operative HPR risk stratification and facilitated clinical decision-making.Multi-dimensional verification also confirmed the predictive accuracy of model combinations and their reliability in clinical applications.Furthermore,biological analyses revealed that HCCs with HPR exhibited hyperactive biological processes,inactive metabolism,and immune exhaustion features,together with high MYCN/HMGA2 co-expression,thereby enhancing understanding of the molecular events leading to HPR and providing valuable knowledge for HPR management.Conclusions:HPR after hepatectomy is associated with extremely poor prognosis and requires substantial attention.We constructed comprehensive predictive models and propose a clinical strategy for guiding HPR prevention and management.展开更多
BACKGROUND Chronic enteropathy associated with solute carrier organic anion transporter family member 2A1(SLCO2A1)(CEAS)is a rare autosomal recessive hereditary disease characterized by anemia,hypoproteinemia,abdomina...BACKGROUND Chronic enteropathy associated with solute carrier organic anion transporter family member 2A1(SLCO2A1)(CEAS)is a rare autosomal recessive hereditary disease characterized by anemia,hypoproteinemia,abdominal pain,diarrhea,and multiple shallow ulcers in the small intestine.Genetic analysis for SLCO2A1 mutations has identified more than 10 variant types,including the mostly reported c.940+1G>A splice site mutation.CASE SUMMARY Herein,we described a 33-year-old female patient who was admitted for anemia,edema,and a positive fecal occult blood test,unaccompanied by abdominal pain and diarrhea.She was diagnosed with CEAS due to compound heterozygous variants,c.940+1G>cA(splice-5)and c.1658T>A(p.Ile553Asn)in SLCO2A1,which had not been previously reported.Importantly,we reviewed 132 reported CEAS patients,which showed that anemia(87.3%)and hypoproteinemia(81%)were the most common symptoms.Nearly 25.8%of patients only had a positive result of fecal occult blood,without any symptoms of gastrointestinal bleeding.CONCLUSION In conclusion,fecal tests should be repeated in patients with anemia and edema to find clues for chronic enteropathy,including the rare cause-CEAS.展开更多
With the popularity of the digital human body,monocular three-dimensional(3D)face reconstruction is widely used in fields such as animation and face recognition.Although current methods trained using single-view image...With the popularity of the digital human body,monocular three-dimensional(3D)face reconstruction is widely used in fields such as animation and face recognition.Although current methods trained using single-view image sets perform well in monocular 3D face reconstruction tasks,they tend to rely on the constraints of the a priori model or the appearance conditions of the input images,fundamentally because of the inability to propose an effective method to reduce the effects of two-dimensional(2D)ambiguity.To solve this problem,we developed an unsupervised training framework for monocular face 3D reconstruction using rotational cycle consistency.Specifically,to learn more accurate facial information,we first used an autoencoder to factor the input images and applied these factors to generate normalized frontal views.We then proceeded through a differentiable renderer to use rotational consistency to continuously perceive refinement.Our method provided implicit multi-view consistency constraints on the pose and depth information estimation of the input face,and the performance was accurate and robust in the presence of large variations in expression and pose.In the benchmark tests,our method performed more stably and realistically than other methods that used 3D face reconstruction in monocular 2D images.展开更多
Transition metal sulfides have great potential as anode mterials for sodium-ion batteries(SIBs)due to their high theoretical specific capacities.However,the inferior intrinsic conductivity and large volume variation d...Transition metal sulfides have great potential as anode mterials for sodium-ion batteries(SIBs)due to their high theoretical specific capacities.However,the inferior intrinsic conductivity and large volume variation during sodiation-desodiation processes seriously affect its high-rate and long-cyde performance,unbeneficial for the application as fast-charging and long-cycling SIBs anode.Herein,the three-dimensional porous Cu1.81Sitrogen-doped carbon frameworks(Cu1.81S/NC)are synthesized by the simple and facile sol-gel and annealing processes,which can accommodate the volumetric expansion of Cu1.81S nanoparticles and accelerate the transmission of ions and electrons during Na+insertion/extraction processes,exhibiting the excellent rate capability(250.6 mA·g-1at 20.0 A·g-1)and outstanding cycling stability(70% capacity retention for 6000 cycles at 10.0 A·g-1)for SIBs.Moreover,the Na-ion full cells coupled with Na3V2(PO4)3/C cathode also demonstrate the satisfactory reversible specific capacity of 330.5 mAh·g-1at 5.0 A·g-1and long-cycle performance with the 86.9% capacity retention at 2.0 A·g-1after 750 cycles.This work proposes a promising way for the conversionbased metal sulfides for the applications as fast-charging sodium-ion battery anode.展开更多
Conductor materials with good mechanical performance as well as high electrical and thermal conductivities are particularly important to break through the current bottle-neck limit(~ 100 T) of pulsed magnets. Here, we...Conductor materials with good mechanical performance as well as high electrical and thermal conductivities are particularly important to break through the current bottle-neck limit(~ 100 T) of pulsed magnets. Here, we perform systematic studies on the elastic properties of the Cu–6wt% Ag alloy wire, which is a promising candidate material for the new-generation pulsed magnets, by employing two independent ultrasonic techniques, i.e., resonant ultrasound spectroscopy(RUS) and ultrasound pulse-echo experiments. Our RUS measurements manifest that the elastic properties of the Cu–6wt% Ag alloy wires can be improved by an electroplastic drawing procedure as compared with the conventional cold drawing. We also take this opportunity to test the availability of our newly-built ultrasound pulse-echo facility at the Wuhan National High Magnetic Field Center(WHMFC, China), and the results suggest that the elastic performance of the electroplastically-drawn Cu–6wt% Ag alloy wire remains excellent without anomalous softening under extreme conditions,e.g., in ultra-high magnetic field up to 50 T and nitrogen or helium cryogenic liquids.展开更多
BACKGROUND Hepatocellular carcinoma(HCC)is the most common pathological type of liver cancer and was the third leading cause of cancer-related deaths worldwide in 2020.AIM To evaluate the diagnostic potential of key t...BACKGROUND Hepatocellular carcinoma(HCC)is the most common pathological type of liver cancer and was the third leading cause of cancer-related deaths worldwide in 2020.AIM To evaluate the diagnostic potential of key tumor markers in serum,bile,and fecal samples for detecting HCC.METHODS Blood,bile,and fecal samples were collected from patients(n=265)with HCC and cholecystitis from Guangxi Medical University’s First Affiliated Hospital.Immunohistochemistry was performed on 69 HCC samples,and 16S ribosomal RNA sequencing was conducted on 166 fecal samples.Tumor marker cut-off values in bile and feces were determined using the Youden index,while serum biomarkers followed hospital standards.Diagnostic performance was evaluated using receiver operating characteristic analysis.RESULTS The areas under the curve(AUCs)for distinguishing HCC were 0.898,0.904,and 0.859 for serum alpha-fetoprotein(AFP),prothrombin induced by vitamin K absence-II(PIVKA-II),and bile AFP,respectively.Serum AFP had the highest diagnostic value(80%)for early-stage HCC.Combination analysis found that bile AFP and serum PIVKAII achieved the highest AUC of 0.965(P<0.001),suggesting that bile AFP may serve as a valuable complementary biomarker,particularly in cases where serum AFP is not significantly elevated.Additionally,bile AFP was positively correlated with Actinomyces,which plays a significant role in promoting tumorigenesis;and was negatively correlated with Faecalibacterium,which was associated with robust anticancer immune responses(P<0.05).These findings suggest the potential role of gut microbiota in modulating AFP levels and HCC progression.CONCLUSION Bile AFP improved the sensitivity of HCC detection,with the combination of bile AFP and PIVKA-II demonstrating the highest AUC for HCC diagnosis.AFP is associated with poorer clinical outcomes.展开更多
基金funded by Scientific Foundation of the Urban Management Bureau of Shenzhen(202403 to YL and 202302 to SD)part of the 10KP project(http://gffzz984746bd41ee48d7spuo5v9v9wu0b6kvn.ffgz.tsg.suse.edu.cn/10kp/)+1 种基金supported by China National GeneBank(CNGBhttp://gffzz6c8c105914b947e9spuo5v9v9wu0b6kvn.ffgz.tsg.suse.edu.cn/).
摘要Modern land plants comprise two main lineages:tracheophytes and bryophytes.The latter include mosses,liverworts,and hornworts.Bryophytes are second only to angiosperms in diversity,with approximately 22,000 species(Shaw,2008).They are characterized by a life cycle dominated by haploid gametophytes,with unbranched diploid sporophytes attached to the gametophytes,producing spores for sexual reproduction(Shaw and Renzaglia,2004).Bryophytes have demonstrated great adaptability to modern ecosystems,thriving in environments ranging from deserts to wetlands and from tropical to polar regions(Degola et al.,2022).This adaptability suggests that bryophytes may have evolved an effective genetic toolkit for stress tolerance.Advances in genome sequencing and assembly technologies offer great opportunities to decipher the genetic toolkit and study the evolution of resistance and environmental adaptation in bryophytes.
摘要Spatially confined microenvironments offer exceptional potential for regulating catalytic activity and selectivity.This study elucidates how the dynamic evolution of carbonaceous species during syngas conversion alters the confined environment within MCM-22 cages.We establish a confinement energy,quantified through ethylene adsorption measurements,as a key descriptor correlating with hydrogenation activity at Brönsted acid sites.As carbonaceous deposits expand in size during syngas reaction,they progressively occupy cage volume and reduce the available space,thereby enhancing confinement energy.Such energy gain universally weakens reactant adsorption,simultaneously suppressing hydrogenation activity and promoting olefin selectivity.Collectively,these findings advance fundamental understanding of dynamic confinement effects and provide valuable insights for further designing high-selectivity catalysts for syngas conversion.
基金supported by the Scientific Foundation of the Urban Management Bureau of Shenzhen(202005,202203,202403 to Yang Liu,and 202106,202302 to Shanshan Dong).
摘要Liverworts,with approximately 7300 species worldwide,exhibit remarkable morphological diversity in terms of growth form,ontogeny,and architecture.Their mitochondrial genome exhibits lower average substitution rates compared to their nuclear and plastid genomes,and shows less structural variation,suggesting its suitability for inferring relationships at higher taxonomic levels.In this study,we substantially expanded mitochondrial sampling in liverworts by adding complete mitochondrial gene sets from 97 species across 25 families,thereby increasing family-level coverage to 71%.Among these,we newly assembled 23 complete mitochondrial genomes.Although four species with structural variants were newly identified,the overall architecture of liverwort mitochondrial genomes remains highly conserved,with taxa that diverged over 470 million years ago still having collinearity.Phylogenetic inferences from mitochondrial genome sequences confirmed the monophyly of most suprafamilial taxa,with the exceptions of Porellales,Ptilidiales,and Pelliidae.Herzogianthus(Ptilidiales)was well-supported as a sister group to Jungermanniales sensu lato,rather than forming a monophyletic lineage with Ptilidium(Ptilidiales).This work provides an important resource for future genetic and phylogenetic studies of liverworts.
摘要Selective electrocatalytic 5-hydroxymethylfurfural(HMF)oxidation reaction(HMFOR)is believed to be highly dependent on the surface adsorption behaviors.Herein,a series of CuAu alloys with various metallic compositions and steered surface adsorption behaviors were prepared by a one-step electrodeposition method.By optimizing the Cu molar ratios,a superior electrocatalytic performance for HMFOR via one-electron dehydrogenation could be observed over Cu0.25Au0.75,reaching a current density of 84.8 mA cm–2at 0.4 V vs.RHE,with 94.5%5-hydroxymethyl-2-furancarboxylic acid(HMFCA)yield,98.6%HMFCA faradaic efficiency(FE)and 94.6%H2FE.Remarkably,with Cu0.25Au0.75as the anode and Pt/C as the cathode,the integrated membrane electrode assembly electrolyzer could operate at a low cell voltage of 0.45 V for simultaneous HMFOR and bipolar H2production,with 94.5%HMFCA FE and~200%H2FE.Experimental investigations and theoretical calculations demonstrate that the alloying induced charge redistribution between Cu and Au could modulate the d-band centers,and then steer the surface adsorption behaviors to balance HMF adsorption and HMFCA desorption at surface,ensuring the active site availability for one-electron dehydrogenation HMFOR.This work presents a facile strategy for designing efficient electrocatalysts for ultralow-potential HMFOR,with fundamental surface adsorption behaviors deepened for biomass electrooxidation.
基金supported by the National Natural Science Foundation of China(Grant Nos.NSFC 82273405,and 81972306)supported partly by the Guangxi Nature Sciences grants(Grant No.2018GXNSFAA138028)the Guangxi Medical University Training Program for Distinguished Young Scholars.
摘要Objective:Hyper-progression recurrence(HPR)after hepatectomy is a specific recurrence pattern associated with extremely poor prognosis in patients with hepatocellular carcinoma(HCC).This study was aimed at investigating the probable risk factors and establishing comprehensive models for formulating clinical strategies.Methods:Overall,16,158 patients with HCC from 8 hospitals were screened,among whom 3,125 patients who underwent R0 resection were included,and divided into development(n=2,113)and validation(n=1,012)cohorts.A comprehensive study of HPR predictive models and biological features was conducted.Results:Among the 3,125 enrolled patients,506(16.19%)developed HPR.The influence of HPR on extremely poor prognosis was reflected by recurrence features,adverse effects on systemic and liver function,and limited therapeutic options.Nine variables closely associated with HPR were identified,and incorporated into nomogram and conditional inference tree models,which successfully achieved pre-and post-operative HPR risk stratification and facilitated clinical decision-making.Multi-dimensional verification also confirmed the predictive accuracy of model combinations and their reliability in clinical applications.Furthermore,biological analyses revealed that HCCs with HPR exhibited hyperactive biological processes,inactive metabolism,and immune exhaustion features,together with high MYCN/HMGA2 co-expression,thereby enhancing understanding of the molecular events leading to HPR and providing valuable knowledge for HPR management.Conclusions:HPR after hepatectomy is associated with extremely poor prognosis and requires substantial attention.We constructed comprehensive predictive models and propose a clinical strategy for guiding HPR prevention and management.
基金Supported by the National Natural Science Foundation of China(General Program),No.82000493Peking University People’s Hospital Scientific Research Development Funds,No.RDJP2023-09.
摘要BACKGROUND Chronic enteropathy associated with solute carrier organic anion transporter family member 2A1(SLCO2A1)(CEAS)is a rare autosomal recessive hereditary disease characterized by anemia,hypoproteinemia,abdominal pain,diarrhea,and multiple shallow ulcers in the small intestine.Genetic analysis for SLCO2A1 mutations has identified more than 10 variant types,including the mostly reported c.940+1G>A splice site mutation.CASE SUMMARY Herein,we described a 33-year-old female patient who was admitted for anemia,edema,and a positive fecal occult blood test,unaccompanied by abdominal pain and diarrhea.She was diagnosed with CEAS due to compound heterozygous variants,c.940+1G>cA(splice-5)and c.1658T>A(p.Ile553Asn)in SLCO2A1,which had not been previously reported.Importantly,we reviewed 132 reported CEAS patients,which showed that anemia(87.3%)and hypoproteinemia(81%)were the most common symptoms.Nearly 25.8%of patients only had a positive result of fecal occult blood,without any symptoms of gastrointestinal bleeding.CONCLUSION In conclusion,fecal tests should be repeated in patients with anemia and edema to find clues for chronic enteropathy,including the rare cause-CEAS.
基金Supported by Science and Technology Department Major Innovation Special Fund of Hubei Province of China(2020BAB116)。
摘要With the popularity of the digital human body,monocular three-dimensional(3D)face reconstruction is widely used in fields such as animation and face recognition.Although current methods trained using single-view image sets perform well in monocular 3D face reconstruction tasks,they tend to rely on the constraints of the a priori model or the appearance conditions of the input images,fundamentally because of the inability to propose an effective method to reduce the effects of two-dimensional(2D)ambiguity.To solve this problem,we developed an unsupervised training framework for monocular face 3D reconstruction using rotational cycle consistency.Specifically,to learn more accurate facial information,we first used an autoencoder to factor the input images and applied these factors to generate normalized frontal views.We then proceeded through a differentiable renderer to use rotational consistency to continuously perceive refinement.Our method provided implicit multi-view consistency constraints on the pose and depth information estimation of the input face,and the performance was accurate and robust in the presence of large variations in expression and pose.In the benchmark tests,our method performed more stably and realistically than other methods that used 3D face reconstruction in monocular 2D images.
基金financially supported by the National Natural Science Foundation of China(Nos.U1904173 and 52272219)the Key Research Projects of Henan Provincial Department of Education(No.19A150043)+2 种基金the Natural Science Foundation of Henan Province(Nos.202300410330 and 222300420276)the Nanhu Scholars Program for Young Scholars of Xinyang Normal Universitythe Xinyang Normal University Analysis&Testing Center。
摘要Transition metal sulfides have great potential as anode mterials for sodium-ion batteries(SIBs)due to their high theoretical specific capacities.However,the inferior intrinsic conductivity and large volume variation during sodiation-desodiation processes seriously affect its high-rate and long-cyde performance,unbeneficial for the application as fast-charging and long-cycling SIBs anode.Herein,the three-dimensional porous Cu1.81Sitrogen-doped carbon frameworks(Cu1.81S/NC)are synthesized by the simple and facile sol-gel and annealing processes,which can accommodate the volumetric expansion of Cu1.81S nanoparticles and accelerate the transmission of ions and electrons during Na+insertion/extraction processes,exhibiting the excellent rate capability(250.6 mA·g-1at 20.0 A·g-1)and outstanding cycling stability(70% capacity retention for 6000 cycles at 10.0 A·g-1)for SIBs.Moreover,the Na-ion full cells coupled with Na3V2(PO4)3/C cathode also demonstrate the satisfactory reversible specific capacity of 330.5 mAh·g-1at 5.0 A·g-1and long-cycle performance with the 86.9% capacity retention at 2.0 A·g-1after 750 cycles.This work proposes a promising way for the conversionbased metal sulfides for the applications as fast-charging sodium-ion battery anode.
基金Project supported by the National Key R&D Program of China (Grant Nos. 2022YFA1602602 and 2023YFA1609600)the National Natural Science Foundation of China (Grant No. U23A20580)+3 种基金the open research fund of Songshan Lake Materials Laboratory (Grant No. 2022SLABFN27)Beijing National Laboratory for Condensed Matter Physics (Grant No. 2024BNLCMPKF004)Guangdong Basic and Applied Basic Research Foundation (Grant No. 2022B1515120020)the interdisciplinary program of Wuhan National High Magnetic Field Center at Huazhong University of Science and Technology (Grant No. WHMFC202132)。
摘要Conductor materials with good mechanical performance as well as high electrical and thermal conductivities are particularly important to break through the current bottle-neck limit(~ 100 T) of pulsed magnets. Here, we perform systematic studies on the elastic properties of the Cu–6wt% Ag alloy wire, which is a promising candidate material for the new-generation pulsed magnets, by employing two independent ultrasonic techniques, i.e., resonant ultrasound spectroscopy(RUS) and ultrasound pulse-echo experiments. Our RUS measurements manifest that the elastic properties of the Cu–6wt% Ag alloy wires can be improved by an electroplastic drawing procedure as compared with the conventional cold drawing. We also take this opportunity to test the availability of our newly-built ultrasound pulse-echo facility at the Wuhan National High Magnetic Field Center(WHMFC, China), and the results suggest that the elastic performance of the electroplastically-drawn Cu–6wt% Ag alloy wire remains excellent without anomalous softening under extreme conditions,e.g., in ultra-high magnetic field up to 50 T and nitrogen or helium cryogenic liquids.
摘要BACKGROUND Hepatocellular carcinoma(HCC)is the most common pathological type of liver cancer and was the third leading cause of cancer-related deaths worldwide in 2020.AIM To evaluate the diagnostic potential of key tumor markers in serum,bile,and fecal samples for detecting HCC.METHODS Blood,bile,and fecal samples were collected from patients(n=265)with HCC and cholecystitis from Guangxi Medical University’s First Affiliated Hospital.Immunohistochemistry was performed on 69 HCC samples,and 16S ribosomal RNA sequencing was conducted on 166 fecal samples.Tumor marker cut-off values in bile and feces were determined using the Youden index,while serum biomarkers followed hospital standards.Diagnostic performance was evaluated using receiver operating characteristic analysis.RESULTS The areas under the curve(AUCs)for distinguishing HCC were 0.898,0.904,and 0.859 for serum alpha-fetoprotein(AFP),prothrombin induced by vitamin K absence-II(PIVKA-II),and bile AFP,respectively.Serum AFP had the highest diagnostic value(80%)for early-stage HCC.Combination analysis found that bile AFP and serum PIVKAII achieved the highest AUC of 0.965(P<0.001),suggesting that bile AFP may serve as a valuable complementary biomarker,particularly in cases where serum AFP is not significantly elevated.Additionally,bile AFP was positively correlated with Actinomyces,which plays a significant role in promoting tumorigenesis;and was negatively correlated with Faecalibacterium,which was associated with robust anticancer immune responses(P<0.05).These findings suggest the potential role of gut microbiota in modulating AFP levels and HCC progression.CONCLUSION Bile AFP improved the sensitivity of HCC detection,with the combination of bile AFP and PIVKA-II demonstrating the highest AUC for HCC diagnosis.AFP is associated with poorer clinical outcomes.