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DNA-based one-pot rapid biosensing platform integrating turn-back loop primer-accelerated LAMP and Pyrococcus furiosus Argonaute for meat visual authentication 认领 引用 被引量：1
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作者 Sheng DING Jing LI +4 位作者 Minglong YANG Yujia SONG Xiangke NIU Mei LI Yao LUO 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS CSCD 2026年第7期775-787,I0020-I0033,共13页
Meat adulteration is a significant global food safety challenge,creating a pressing need for rapid and on-site detection technologies.Herein,we present an intelligent one-pot biosensing platform termed one-pot TLAMP-P... Meat adulteration is a significant global food safety challenge,creating a pressing need for rapid and on-site detection technologies.Herein,we present an intelligent one-pot biosensing platform termed one-pot TLAMP-PfAgo assay(OTPA)that integrates the rapid amplification of turn-back loop primer-accelerated loop-mediated isothermal amplification(LAMP)(TLAMP)with the sequence-specific detection of Pyrococcus furiosus Argonaute(PfAgo).This system features a clever heat-activatable design using microcrystalline wax to spatially separate reactions within a single tube,enabling contamination-free and streamlined operation.The OTPA assay achieves sensitive and specific detection,with limits of detection as low as 3×10-4 ng/μL for pork DNA and 2×10-4 ng/μL for beef DNA within 30 min.It successfully enables duplex target identification and has been validated with commercial meat products,showing perfect concordance with standard polymerase chain reaction(PCR)-based qualitative detection.Notably,the result can be directly visualized under blue light,underscoring the substantial potential of this costeffective and simple platform for point-of-care testing(POCT)and intelligent biosensing in food safety surveillance. 展开更多
关键词 Loop-mediated isothermal amplification(LAMP) Turn-back loop primer-accelerated LAMP(TLAMP) Pyrococcus furiosus Argonaute(PfAgo) Meat authentication One-pot assay Point-of-care testing(POCT)
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Orthogonal Stimulus-Responsive Ca3Ga2Ge3O12:Pr3+for Real-Time X-Ray Dosimetry and Dynamic Information Encryption 认领 引用
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作者 Qi Chen Wenqiang Wang +8 位作者 Ping Liu Weifang Bu Yang Yue Yifan Shang Feixiang Xiong Ting Wang Xiangke Niu Zhiqiang Li Xue Yu 《Rare Metals》 SCIE EI CAS CSCD 2026年第3期422-429,共8页
Multimodal luminescent materials are crucial for advanced information encryption,real-time dosimetry,and high-resolution bioimaging,yet integrating orthogonal stimulus-responsiveness into a single-component system is ... Multimodal luminescent materials are crucial for advanced information encryption,real-time dosimetry,and high-resolution bioimaging,yet integrating orthogonal stimulus-responsiveness into a single-component system is challenging.Herein,we report a Pr3+-doped Ca3Ga2Ge3O12(CGGO:Pr3+)garnet phosphor that exhibits orthogonally addressable luminescence under ultraviolet(UV)light,X-ray radiation,and thermal stimulation.This phosphor demonstrates dual spectrally distinct emissions with relative intensities that can be precisely modulated by varying the excitation wavelength or ambient temperature,enabling color tuning from blue to orange.Furthermore,time-resolved multi-color afterglow after UV or X-ray pre-excitation,along with thermally stimulated luminescence,provides complementary readout channels.Notably,CGGO:Pr3+exhibits dose-rate-and temperature-dependent color evolution(from blue-white to orange-white)under concurrent UV and X-ray irradiation,facilitating real-time naked-eye monitoring of both X-ray dose rate and temperature.By leveraging these orthogonal response modes,we demonstrate visual real-time X-ray dose and temperature detection,high-security X-ray imaging,and 3D-encrypted quick response codes.This study establishes CGGO:Pr3+as a versatile single-component platform for orthogonal stimuli-responsive applications,advancing the fields of dynamic information encryption and instantaneous X-ray dose-rate visualization. 展开更多
关键词 dynamic information encryption orthogonal stimulus-responsiveness X-ray dosimetry
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Innovations in hydrogel therapies for diabetic wound healing:bridging the gap between pathophysiology and clinical application 认领 引用
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作者 Yao Wang Haoming Wu +10 位作者 Yan Pan Yibo Xiao Yingying Chen Shuhao Yang Jun Wang Wanyue Feng Cheng Hu Xiangke Niu Xin Yong Jin Yang Xulin Hu 《Burns & Trauma》 SCIE CSCD 2025年第1期834-872,共39页
Diabetic wounds(DWs),which are complex and challenging to treat due to delayed healing and incomplete regeneration,pose a significant burden on global healthcare systems.Existing clinical interventions,which mainly co... Diabetic wounds(DWs),which are complex and challenging to treat due to delayed healing and incomplete regeneration,pose a significant burden on global healthcare systems.Existing clinical interventions,which mainly comprise debridement,decompression,and wound dressings,have limited efficacy.In addition,DW pathogenesis is complex,with diabetic peripheral neuropathy,diabetic peripheral arterial disease,and diabetic foot infections further complicating wound management.Owing to their unique versatility,tunability,and hydrophilicity,hydrogels show promise in several biomedical applications,including DW management.They can effectively promote DW healing by loading therapeutic substances for on-demand release.Given the distinct physiological milieu of DWs,hydrogels with tailored attributes can be engineered to enable ondemand drug release,optimize the wound microenvironment,and cater to the diverse stages of wound healing.Based on the clinical status and pathophysiological features of DWs,this review explores hydrogel wound dressings with the following effects:hypoglycemic,nerve regeneration,vascular regeneration,anti-infective,and bone repair.Additionally,the strategy for applying hydrogels to DWs has been comprehensively studied to provide a robust theoretical foundation for DW treatment and pave the way for clinical translation. 展开更多
关键词 Diabetic wounds Wound healing Biomaterials Hydrogels Regenerative medicine
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