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Simultaneous removal of manganese and nitrate by manganese redox driven moving bed biofilm reactor:Characteristic,biological community and mechanism 认领 引用
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作者 Tenghao Li Weiwu Shen +3 位作者 Yiyang Zhou Sicheng Shao Xiangwei Wu Dandan Pan 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2026年第3期858-871,共14页
This study investigated the effects of different manganese forms(MnCl2and MnO2)on the nitrogen and phosphorus removal performance of moving bed biofilm reactor(MBBR).Compared to the control without manganese,the... This study investigated the effects of different manganese forms(MnCl2and MnO2)on the nitrogen and phosphorus removal performance of moving bed biofilm reactor(MBBR).Compared to the control without manganese,the addition of MnCl2and MnO2increased NO3--N removal efficiency by 11.47%and 9.54%,and total nitrogen(TN)removal efficiency by 17.91%and 15.45%,respectively.The average accumulation of NO2--N decreased from 3.02 to 0.04 mg/L and 0.18 mg/L,respectively.The manganese redox system induced by MnCl2enhanced total phosephorus(TP)removal efficiency by approximately 3.5 times,while MnO2reduced TP removal efficiency by 2.94%.After discontinuing MnCl2and MnO2supplementation,denitrification efficiency significantly declined,and Mn(Ⅱ)reduced by BioMnOx could not sustain the manganese cycling process long-term.Extracellular polymeric substances(EPS)analysis revealed that MnCl2and MnO2stimulated the production of uronic acids,amideⅢ,and secondary amides in proteins.High-throughput sequencing indicated that Proteobacteria,Bacteroidetes,Chloroflexi,and Acidobacteria were the dominant phyla involved in denitrification,but different manganese sources altered the microbial community composition.The relative abundance of Proteobacteria generally decreased,while Bacteroidetes increased by 16.29%and 4.14%with MnCl2and MnO2.MnCl2was more conducive to the Bacteroidetes growth.This study provides a practical framework for applying manganeseenhanced MBBR system in wastewater treatment plants to improve nitrogen removal efficiency and operational stability. 展开更多
关键词 Denitrification Manganese redox cycling Moving bed biofilm reactor Microbial community Extracellular polymeric substances
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Influence of oxygen content on the microstructure and mechanical properties of cold rolled Ti-32.5Nb-6.8Zr-2.7Sn-x O alloys after aging treatment 认领 引用 被引量：7
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作者 Chunbo Lan Feng Chen +2 位作者 Huijuan Chen Yulong Wu Xiangwei Wu 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2018年第11期2100-2106,共7页
The influence of oxygen（O） content on the microstructure and mechanical properties of cold rolled Ti-32.5 Nb-6.8 Zr-2.7 Sn-xO（TNZS-xO; x = 0,0.3.0.6; mass%） alloys after aging at temperature range from 350 to 600... The influence of oxygen（O） content on the microstructure and mechanical properties of cold rolled Ti-32.5 Nb-6.8 Zr-2.7 Sn-xO（TNZS-xO; x = 0,0.3.0.6; mass%） alloys after aging at temperature range from 350 to 600℃ for 24 h was investigated. Results showed that the cold rolled TNZS-xO alloys possess single βphase. During the aging process, O could not only effectively suppress the precipitation of ω phase but also retard the formation and decomposition of a phase. In addition, the corresponding temperatures of the maximal volume fraction of α phase precipitation and the（α＋β）/β transus temperatures of the TNZS-xO alloys were all increased with the increasing of O content. For mechanical properties, it was found that the strength and Young＇s modulus of the TNZS-xO alloys increased owing to the ω phase and/or a phase precipitation and decreased owing to the a phase decomposition. However, the elongation showed the opposite change tendency with the above mentioned strength. The mechanical properties of TNZS-xO alloys can be controlled over a wide range by subjecting to aging treatment and/or changing their 0 content. When aged at or below 450 ℃,the TNZS-xO alloys exhibit great potential to become a series of new candidates for biomedical applications since they possess high strength（870-1460 MPa）, low Young＇s modulus（45.1-75.6 GPa）, high strength-to-modulus ratio（0.018-0.02） and appropriate elongation（7.2%-14.9%）, which are superior to those of Ti-6 Al-4 V alloy and suitable for the use as bio-implant materials. 展开更多
关键词 Ti-32,5Nb-6.8Zr-2.7Sn-xO alloys Aging treatment Microstructure Mechanical properties
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Combined remediation of DDT congeners and cadmium in soil by Sphingobacterium sp.D-6 and Sedum alfredii Hance 认领 引用 被引量：5
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作者 Hua Fang Wei Zhou +7 位作者 Zhengya Cao Feifan Tang Dandan Wang Kailin Liu Xiangwei Wu Xiao'e Yang Yongge Sun Yunlong Yu 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2012年第6期1036-1046,共11页
Combined pollution of 1,1,1-trichloro-2,2-bis （4-chlorophenyl） ethane （DDT） and cadmium （Cd） in agricultural soils is of great concern because they present serious risk to food security and human health.In order... Combined pollution of 1,1,1-trichloro-2,2-bis （4-chlorophenyl） ethane （DDT） and cadmium （Cd） in agricultural soils is of great concern because they present serious risk to food security and human health.In order to develop a cost-effective and safe method for the removal of DDTs and Cd in soil,combined remediation of DDTs and Cd in soil by Sphingobacterium sp.D-6 and the hyperaccumulator,Sedum alfredii Hance was investigated.After treatment for 210 days,the degradation half-lives of DDTs in soils treated by strain D-6 decreased by 8.1% to 68.0% compared with those in the controls.The inoculation of strain D-6 into soil decreased the uptake of DDTs by pak choi and S.alfredii.The shootsoots ratios of S.alfredii for the Cd accumulation ranged from 12.32 to 21.75.The Cd concentration in soil decreased to 65.8%-71.8% for S.alfredii treatment and 14.1%-58.2% for S.alfredii and strain D-6 combined treatment,respectively,compared with that in the control.The population size of the DDTs-degrading strain,Simpson index （1/D） and soil respiratory rate decreased in the early stage of treatment and then gradually increased,ultimately recovering to or exceeding the initial level.The results indicated that synchronous incorporation of strain D-6 and S.alfredii into soil was found to significantly （p 0.05） enhance the degradation of DDTs in soil and the hyperaccumulation of Cd in S.alfredii.It was concluded that strain D-6 and S.alfredii could be used successfully to control DDTs and Cd in contaminated soil. 展开更多
关键词 bioremediation cadmium Sphingobacterium sp. Sedum alfredii Hance 1,1,1-trichloro-2,2-bis （4-chlorophenyl） ethane
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Role,residues and microbial degradation of plant growth regulators(PGRs):A scoping review 认领 引用 被引量：1
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作者 Zhaoxian Zhang Sicheng Shao +1 位作者 Dandan Pan Xiangwei Wu 《Advanced Agrochem》 CAS 2024年第1期43-46,共4页
Plant growth regulators(PGRs)play an important role in increasing crop yield,and quality,and enhancing crop stress resistance in agricultural production,especially for important crops.PGRs can affect the transport and... Plant growth regulators(PGRs)play an important role in increasing crop yield,and quality,and enhancing crop stress resistance in agricultural production,especially for important crops.PGRs can affect the transport and distribution of assimilates by changing the content and distribution of endogenous hormones in plants.Numerous empirical research results have proven that PGRs have an important impact on the growth,development,and yield composition of wheat.Taking wheat plants as an example,this study reviews the application of PGRs in wheat production and explores their impact on wheat growth and yield.Furthermore,residues and microbial degradation of PGRs are summarized in detail.Finally,future research directions on PGR application in wheat production are proposed.This summary is of great significance for understanding the role of PGRs in wheat production. 展开更多
关键词 Agricultural production Crop growth Plant growth regulators(PGRs) Wheat
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Pretreatment with antioxidants prevent bone injury by improving bone marrow microenvironment for stem cells 认领 引用 被引量：1
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作者 Lingling Xian Michael Lou +8 位作者 Xiangwei Wu Bing Yu Frank Frassica Mei Wan Lijuan Pang Chunyi Wen Erik Tryggestad John Wong Xu Cao 《Stem Cell Discovery》 2012年第3期100-107,共8页
Irradiation induces bone injury by generating free radicals that adversely affect the microenvironment for Mesenchymal stem cells (MSCs) and damages bone marrow blood vessels. We wished to investigate the efficacy of ... Irradiation induces bone injury by generating free radicals that adversely affect the microenvironment for Mesenchymal stem cells (MSCs) and damages bone marrow blood vessels. We wished to investigate the efficacy of antioxidant administration in protecting stem cell microenvironments and promoting bone marrow vasculature recovery after radiation treatment. The antioxidant ascorbic acid was administered 3 times at a dosage: 150 mg/kg/day to experimenttal groups 3 days before targeted radiation by a unique Small Animal Radiation Research Platform (SARRP). Histological staining indicated that antioxidant treated mice had less severe bone marrow damage 1 week after irradiation with substantial marrow cellular recovery 4 weeks later. Flow cytometry analysis showed that antioxidant administration was correlated with a rebound in MSC quantity in bone marrow. Anti-oxidant treatment was also observed to allow for better vasculature retention and recovery through angiographic imaging. Our data suggests that pre-treatment with ascorbic acid serves to improve bone marrow microenvironments for bone marrow stem cells after radiation treatment. 展开更多
关键词 Irradiation Bone Injury Stem Cells Microenvironment Antioxidant
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Synthesis, Characterization, and Antifungal Evaluation of Thiolactomycin Derivatives 认领 引用
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作者 Pei Lv Yiliang Chen +3 位作者 Dawei Wang Xiangwei Wu Qing XLi Rimao Hua 《Engineering》 SCIE EI CAS 2020年第5期560-568,共9页
5-Substituted benzylidene 3-acylthiotetronic acids are antifungal.A series of 3-acylthiotetronic acid derivatives with varying substitutions at the 5-position were designed,synthesized,and characterized,based on the b... 5-Substituted benzylidene 3-acylthiotetronic acids are antifungal.A series of 3-acylthiotetronic acid derivatives with varying substitutions at the 5-position were designed,synthesized,and characterized,based on the binding pose of 3-acyl thiolactone with the protein C171Q KasA.Fungicidal activities of these compounds were screened against Valsa Mali,Curvularia lunata,Fusarium graminearum,and Fusarium oxysporum f.sp.lycopersici.Most target compounds exhibited excellent fungicidal activities against target fungi at the concentration of 50μg·mL-1.Compounds 11c and 11i displayed the highest activity with a broad spectrum.The median effective concentration(EC50)values of 11c and 11i were 1.9–10.7 and 3.1–7.8μg·mL-1,respectively,against the tested fungi,while the EC50 values of the fungicides azoxystrobin,carbendazim,and fluopyram were respectively 0.30,4.22,and>50μg·mL-1 against V.Mali;6.7,41.7,and 0.18μg·mL-1 against C.lunata;22.4,0.42,and 0.43μg·mL-1 against F.graminearum;and 4.3,0.12,and>50μg·mL-1 against F.oxysporum f.sp.lycopersici.The structures and activities of the target compounds against C.lunata were analyzed to obtain a statistically significant comparative molecular field analysis(CoMFA)model with high prediction abilities(q2=0.9816,r2=0.8060),and its reliability was verified.The different substituents on the benzylidene at the 5-position had significant effects on the activity,while the introduction of a halogen atom at the benzene ring of benzylidene was able to improve the activity against the tested fungi. 展开更多
关键词 3-Acylthiotetronic acid Fungicide Quantitative structure-activity relationship Antifungal activity
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SnO2-SnS2/graphene heterojunction composite promotes highperformance sodium ion storage 认领 引用
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作者 Yuanyuan Ma Ke Ping +6 位作者 Peng Sun Kaibin Lin Junjie Guo Lu Yue Wenhui Zhang Xiangwei Wu Zhaoyin Wen 《Journal of Materiomics》 SCIE CSCD 2025年第4期325-339,共15页
The design of electrode material nanostructures including reducing material sizes and designing appropriate heterostructures,has great potential in improving charge storage dynamics and enhancing practical performance... The design of electrode material nanostructures including reducing material sizes and designing appropriate heterostructures,has great potential in improving charge storage dynamics and enhancing practical performance.In this study,we present the innovative synthesis of SnO2-SnS2/graphene heterojunction composite materials via a controlled vulcanization reaction process.The unique structure endows the composite with high electronic conductivity,rapid ion diffusion rates,elevated electrochemical activity,excellent structural stability,and abundant reaction sites,making it a highly efficient anode material for sodium-ion batteries(SIBs).Half-cell tests demonstrate that the SnO2-SnS2-G composite achieves a first Coulombic efficiency of 77.3%at a high current density of 5 A/g,showing remarkable cycling stability.Remarkably,the composite retains a reversible capacity of 330 mA,h/g after 1000 cycles,with a capacity retention rate of 77.5%.Moreover,we elucidate the specific sodium storage mechanisms of the heterojunction composite electrode via in-situ and ex-situ characterization methods.Furthermore,a full battery utilizing Na0.53MnO2as the cathode and SnO2-SnS2-G composite as the anode exhibits outstanding rate performance and long-term cycling stability.This method of heterostructure design and fabrication,coupled with the exceptional performance metrics,suggests that the SnO2-SnS2-G heterostructure is a promising candidate for advanced anode materials in SIBs applications. 展开更多
关键词 SnO2-SnS2/Graphene composite Heterojunction Electrochemical performance Anode material SIBs
Safe transportation and targeted destruction:Albumin encapsulated aggregation-induced emission photosensitizer nanoaggregate for tumor photodynamic therapy through mitochondria damage-triggered pyroptosis 认领 引用 被引量：7
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作者 Juanmei Cao Yong Qu +10 位作者 Shaojie Zhu Jinshan Zhan Yiting Xu Yifan Jin Yuqing Wang Zhuoxia Li Chuxing Chai Xiangwei Wu Meng Gao Changzheng Huang Min Li 《Aggregate》 EI CAS 2024年第6期379-391,共13页
Photodynamic therapy is a highly recommended alternative treatment for solid tumors,such as cutaneous or luminal tumors,in clinical practice.However,conventional photosensitizers(PSs)often induce undesirable phototoxi... Photodynamic therapy is a highly recommended alternative treatment for solid tumors,such as cutaneous or luminal tumors,in clinical practice.However,conventional photosensitizers(PSs)often induce undesirable phototoxic effects because of their normal tissue distribution and a reduction in antitumor effects resulting from aggregation-caused quenching effects.The present study developed a novel nanoformulated aggregation-induced emission(AIE)-characteristic PS,nab-TTVPHE,which is composed of human serum albumin as a carrier and TTVPHE as a therapeutic agent,as a more effective cancer treatment with lower phototoxic effects.Notably,the reactive oxygen species generated by TTVPHE were shielded by the nanoaggregate structure,and the photodynamic activity was after nanostructure dissociation.Nab-TTVPHE was actively internalized in tumor cells via secreted protein,acidic and rich in cysteine and released to form nanoaggregates.TTVPHE accumulated in mitochondria,where it triggered mitochondrial damage under light irradiation via its photodynamic activity and induced pyroptosis via the caspase-3/gasdermin E(GSDME)signaling pathway to kill tumor cells.Therefore,this nano-formulated AIE-characteristic PS provides an innovative strategy for cancer treatment with lower phototoxic effect and the ability to boost potential antitumor immunity via GSDME-mediated pyroptosis. 展开更多
关键词 aggregation-induced emission gasdermin E mitochondria damage nanoparticle albumin-bound photodynamic therapy pyroptosis
Glycomolecules in Echinococcus granulosus cyst fluid inhibit TLR4-mediated inflammatory responses via c-Raf 认领 引用 被引量：6
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作者 Jun Hou Linlin Li +7 位作者 Dan Dong Lianghai Wang Xian Wang Kun Yang Xiaodan Xu Congzhe Chen Xiangwei Wu Xueling Chen 《Cellular & Molecular Immunology》 SCIE CAS CSCD 2020年第4期423-425,共3页
Cystic echinococcosis,which is caused by larval infection of Echinococcus granulosus(E.granulosus)sensu lato,is a widespread chronic cyst-forming helminthic disease that affects up to 3 million people.1 E.granulosus c... Cystic echinococcosis,which is caused by larval infection of Echinococcus granulosus(E.granulosus)sensu lato,is a widespread chronic cyst-forming helminthic disease that affects up to 3 million people.1 E.granulosus can actively divert the host immune response towards anergy and anti-inflammatory pathways.2 Previous studies have shown that E.granulosus can escape host immunosurveillance by modulating the maturation of dendritic cells(DCs).3,4 Nevertheless,how E.granulosus,an extracellular pathogen,modulates the inflammatory response in DCs remains poorly understood.In this study,we investigated the effects of E.granulosus cyst fluid(EgCF)on bone marrow-derived DCs(BMDCs)exposed to LPS,an inflammatory stimulus,and the mechanisms involved in the EgCF–DCs immune modulation. 展开更多
关键词 fluid inflammatory Echinococcus
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蛋白质化学合成在蛋白质泛素化研究中的应用 认领 引用 被引量：2
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作者 武世典 梁家伟 +2 位作者 吴祥伟 邓智亨 刘磊 《中国科学：化学》 CAS CSCD 北大核心 2025年第4期825-846,共22页
泛素化修饰调控真核细胞中绝大多数的生理与病理过程.在分子水平上深入解析蛋白质的泛素化过程能够提升人们对泛素化相关疾病的认知,促进新型诊疗策略的开发.蛋白质化学合成作为获取难以生物重组蛋白的策略,可获取各种定点泛素化修饰的... 泛素化修饰调控真核细胞中绝大多数的生理与病理过程.在分子水平上深入解析蛋白质的泛素化过程能够提升人们对泛素化相关疾病的认知,促进新型诊疗策略的开发.蛋白质化学合成作为获取难以生物重组蛋白的策略,可获取各种定点泛素化修饰的蛋白体系,为泛素化机制的解析研究提供了重要方法基础.本文总结了蛋白质化学合成中所涉及的关键技术,包括多肽固相合成、蛋白片段连接以及蛋白折叠复性等.借助蛋白质化学合成,人们实现了泛素链、泛素化底物蛋白、泛素化学探针等研究工具的高效制备,并在此基础上阐明了一系列泛素化酶、去泛素化酶及靶向泛素系统的小分子药物的工作机制.蛋白质化学合成在泛素化研究中的广泛应用展示了化学生物学工具在推动人类健康与疾病研究、促进药物开发与精准治疗中的重要作用. 展开更多
关键词 翻译后修饰 泛素化 蛋白质化学合成
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Design of solid-state sodium-ion batteries with high mass-loading cathode by porous-dense bilayer electrolyte 认领 引用 被引量：2
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作者 Hongjian Lai Yanpei Li +3 位作者 Jingyi Wang WenWen Li Xiangwei Wu Zhaoyin Wen 《Journal of Materiomics》 SCIE 2021年第6期1352-1357,共6页
Solid-state sodium-ion batteries with sodium metal anodes possess high safety and reliability,which are considered as a promising candidate for the next generation of energy storage technology.However,poor electronic ... Solid-state sodium-ion batteries with sodium metal anodes possess high safety and reliability,which are considered as a promising candidate for the next generation of energy storage technology.However,poor electronic and ionic conductivities at the interface between electrodes and solid-state electrolytes restrict its practical application.Herein,we demonstrate a β″-Al2O3 electrolyte with a vertically porousdense bilayer structure to solve this problem.The carbon-coated vertically porous layer serves as a high mass-loading host for Na3V2(PO4)3 cathode and provides fast electronically and ionically conductive pathways.In addition,the dense layer is produced to prevent sodium dendrite growth and improve mechanical strength of β″-Al2O3 electrolyte.Experimental results show that the cathode loading in vertically porous layer can reach to 8 mg cm-2,and the porous-dense bilayer β″-Al2O3 electrolyte-based battery exhibits a reversible specific capacity of 87 mAh g-1 and a capacity retention of 95.5%over 100 cycles at a current density of 0.1 C,which is superior to that of the traditional dense β″-Al2O3 electrolytebased battery.This work based on electrolyte structure design represents an efficient strategy for the development of solid-state sodium-ion batteries with high mass-loading cathode. 展开更多
关键词 Solid-state sodium-ion batteries Electrolyte structure design Porous-dense bilayerβ″-Al2O3electrolyte High mass-loading cathode
Chinese guidelines for minimally invasive donor hepatectomy in living donor liver transplantation(2024 edition) 认领 引用 被引量：2
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作者 Xi Xu Tao Lv +99 位作者 Gang Xu Qiang Wei Qi Ling Lin Wei Jianhua Li Jiakai Zhang Yang Cai Jinzhen Cai Guihua Chen Zhishui Chen Zhonghua Chen Ying Cheng Jian Dou Shunda Du Chengyou Du Zhiren Fu Zhiyong Guo Lianghui Gao Xiaoshun He Qiang He Lei Huang Jianzhao Huang Feng Huo Changku Jia Chen Jin Wentao Jiang Jipin Jiang Zuoyi Jiao Hong-En Jing Ren Lang Bo Li Li Li Ning Li Qiyong Li Wengang Li Yumin Li Guangming Li Jun Liu Lei Liu Jingfeng Liu Lianxin Liu Zuojin Liu Shichun Lu Qian Lu Ling Lv Yi Lv Guoyue Lv Tingbo Liang Yingzi Ming Zhihai Peng Jianghua Ran Jun Shi Beicheng Sun Chengyi Sun Xuyong Sun Yuling Sun Zhongzhou Si Yingmei Shao Jiulin Song Kaishan Tao Mujian Teng Yunle Wan Xueshuai Wan Liming Wang Hao Wen Gang Wu Jian Wu Xiangwei Wu Zhongjun Wu Lai Wei Jian Xu Jun Xu Yang Yang Hongji Yang Zhanyu Yang Zhaoxu Yang Jian Yang Qifa Ye Shuhong Yi Jian Zhou Feng Zhang Lei Zhang Min Zhang Wu Zhang Leida Zhang Shuijun Zhang Shunyun Zhao Hong Zheng Lin Zhong Haitao Zhu Jiye Zhu Xiaofeng Zhu Zhijun Zhu Hong Wu Wenzhi Guo Zhengxin Wang Xiao Xu Jiayin Yang Branch of Organ Transplant Physicians of Chinese Medical Doctor Association Branch of Organ Transplant of Chinese Medical Association 《Hepatobiliary Surgery and Nutrition》 SCIE 2024年第6期919-936,共18页
Background:Minimally invasive surgeries are increasingly central to modern medicine,particularly in liver transplantation.These techniques,which offer reduced trauma,precise operations,minimal bleeding,and swift recov... Background:Minimally invasive surgeries are increasingly central to modern medicine,particularly in liver transplantation.These techniques,which offer reduced trauma,precise operations,minimal bleeding,and swift recovery,are,however,unevenly adopted across China.Only a limited number of centers routinely perform minimally invasive donor hepatectomies,indicating a significant imbalance in the development and application of these advanced procedures.Additionally,there lacks a set of standardized guidelines that are tailored to meet China’s unique healthcare challenges and conditions.Methods:In August 2023,the Branch of Organ Transplant of Chinese Medical Association and the Branch of Organ Transplant Physicians of Chinese Medical Doctor Association convened a group of national liver transplantation experts to establish a guideline development committee.This committee conducted a thorough review of relevant literature,evaluated existing guidelines and consensus,and assessed factors such as the evidence base,patient preferences,and the cost-effectiveness of interventions within China.After multiple rounds of discussions,both online and offline,the committee finalized the guidelines.Results:This collaborative effort led to the creation of the“Chinese guidelines for minimally invasive donor hepatectomy in living donor liver transplantation(2024 edition)”.These guidelines address crucial aspects such as the safety and advantages of minimally invasive surgery for living donor liver transplantation,donor selection criteria,anesthesia strategies,surgical technical details,and learning curves associated with these procedures,resulting in a comprehensive set of 26 recommendations.Conclusions:The formulation of these guidelines represents a significant advancement towards standardizing minimally invasive liver transplantation surgeries in China.They are designed to enhance outcomes for both donors and recipients by synthesizing expert consensus with contemporary research and clinical practices.Moreover,they serve as a crucial reference for surgeons and medical institutions,promoting the refinement and adoption of minimally invasive surgical techniques in liver transplantation. 展开更多
关键词 Living donor liver transplantation donor minimally invasive surgery guideline
Temperature stress response of heat shock protein 90(Hsp90)in the clam Paphia undulata 认领 引用
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作者 Xiangyang Lin Xiangwei Wu Xiande Liu 《Aquaculture and Fisheries》 2018年第3期106-114,共9页
The Heat shock proteins(HSPs)are a group of molecular chaperones that play a crucial role in cell response to various stresses.A full-length cDNA of the heat shock protein 90(PuHsp90)was cloned and sequenced from the ... The Heat shock proteins(HSPs)are a group of molecular chaperones that play a crucial role in cell response to various stresses.A full-length cDNA of the heat shock protein 90(PuHsp90)was cloned and sequenced from the clam Paphia undulata.Phylogenetic analysis revealed that PuHsp90 grouped with the Hsp90 from other metazoan species.Expression of PuHsp90 was highly detected in the gonad,followed by digest gland,gills and heart but was found poorly expressed in mantle,adductor muscle and hemocytes.After a heat shock stress at 32℃up-regulation of PuHsp90 was detected in the mantle,adductor muscle,gills and hemocytes.Maximal expression levels occurred at 4 h after the heat shock and up-regulation is indicative of protein denaturation and of an increase in energy consumption.In contrast after the heat shock,PuHsp90 was continuously down-regulated in the digestive gland and in the gonad suggesting modifications of the biochemical pathways and energy budgets involved in the synthesis of other protein,such as catalase and of other Hsp proteins.These results reveal that PuHsp90 may play an important role in the clam response to a temperature stress. 展开更多
关键词 Paphia undulata Clam Heat shock protein 90 Expression Heat shock Stress
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