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Advancing Automated Liquid-Handling Systems:Development and Evaluation of a Nonlinear Error-Compensated Air-Displacement Pipettor 认领 引用
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作者 Jun Lu Zhizhong Zhang +13 位作者 Qingqing Ye Sheng Zhang Jianqiang Chen Yuanhua Wang Guodong Sun Zhiyong Sun Jisheng Qin Jianhua Dong Shiwen Mai Fengxiang Zhang Xiaohui Wen Xiao Zhang Guanglei Zhao Xueping Chen 《Instrumentation》 2026年第1期57-74,共18页
This study introduces a nonlinear error-compensated air-displacement pipettor(NEC_ADP),a novel system that addresses the key limitations in commercial pipetting setups.By incorporating nonlinear error-compensation(NEC... This study introduces a nonlinear error-compensated air-displacement pipettor(NEC_ADP),a novel system that addresses the key limitations in commercial pipetting setups.By incorporating nonlinear error-compensation(NEC)technology,the NEC_ADP improves the accuracy and precision of liquid handling across a wide range of volumes,from micro-volumes(1μL)to macro-volumes(up to 1000μL),and for reagents with varying viscosities and surface tensions.Unlike conventional pipettors,which rely on linear compensation or manual recalibration,NEC_ADP features real-time,online calibration,eliminating the need for factory recalibration and reducing maintenance costs.The system was built with a modular design,allowing seamless scalability from single-to multi-channel configurations.It integrates effortlessly with existing laboratory systems using the open platform communications unified architecture(OPC UA),enhancing interoperability and automation.In addition,the use of machine-learning algorithms for motion control and trajectory planning ensures optimal pipetting strategies and automatic adaptation to different reagents and volumes.This study demonstrates the superior performance of NEC_ADP compared to commercial systems,including the TECAN Cavro®and Eppendorf epMotion®,with significant improvements in accuracy and precision.Innovation in NEC_ADP technology and system integration marks a significant advancement in automated liquid handling,offering robust support for highprecision applications in fields such as genomics,stemcell research,and synthetic biology. 展开更多
关键词 nonlinear error compensated air displacement pipettor(NEC_ADP) open platform communications unified architecture(OPC UA) automated liquid handling curve regression and motion control online accuracy calibration
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Factors influencing the occurrence and distribution of organophosphate esters in sediments by cascade reservoirs of the Wujiang River in Southwest China 认领 引用
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作者 Xiaoying Li Yujie Wang +5 位作者 Yong Qin Yongsheng Chu Xueping Chen Ming Yang Jing Ma Fushun Wang 《Acta Geochimica》 EI CAS CSCD 2026年第2期205-216,共12页
An artificial reservoir,as a temporary host for various materials in a basin,faces the potential risk of organophosphate ester(OPE)enrichment in the sediment.In this study,in order to investigate the impacts of damrel... An artificial reservoir,as a temporary host for various materials in a basin,faces the potential risk of organophosphate ester(OPE)enrichment in the sediment.In this study,in order to investigate the impacts of damrelated water impoundment on OPEs,seven reservoirs in the Wujiang River(WJR)were investigated in January,April,July,and October 2017,and the occurrence and distribution of eight OPEs were evaluated.The results showed that the content of the eight OPEs ranged from 0.83 to 2745 ng/g dw.The main component was tris(2-chloroethyl)phosphate(TCEP).Spatially,the OPEs decreased from upstream to downstream.Temporally,the OPE content showed a trend of July>October≥January>April.Socioeconomic and hydrological factors had significant effects on the distribution of OPEs,and the empirical model of OPEs with gross domestic product(GDP)and reservoir age(RA)as parameters was established.This provided a basis for OPE estimation and treatment direction for the reservoir.In addition,the relevant data regarding OPEs and total organic carbon(TOC)in terrigenous sediments from previous studies showed a significant correlation(r=0.63,P<0.01).The significantly high risk quotient(RQ)estimated for OPEs,especially tris(methylphenyl)phosphate(TMPP)and triphenyl phosphate(TPHP),suggest potential adverse risk. 展开更多
关键词 Organophosphate esters(OPEs) Cascade reservoirs Surface sediment Wujiang River TOC Reservoir hydrology
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PUFECBENE®:A microencapsulated pterostilbene with enhanced bioavailability for skin depigmentation 认领 引用
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作者 Zongxiao Cen Zhiyuan Chen +3 位作者 Ding Wang Yuqin Zuo Junyuan Chen Xueping Chen 《Journal of Dermatologic Science and Cosmetic Technology》 2026年第1期59-66,共8页
Pterostilbene,a bioactive compound with multifaceted therapeutic potential,has garnered significant interest in cosmetic applications for its depigmentation properties.However,its clinical utility is limited by poor a... Pterostilbene,a bioactive compound with multifaceted therapeutic potential,has garnered significant interest in cosmetic applications for its depigmentation properties.However,its clinical utility is limited by poor aqueous solubility and bioavailability.To address this,we developed PUFECBENE®,a novel microencapsulated formulation containing 5%pterostilbene(the rest are 30%aqua,48.5%glycerin,10%caprylic/capric triglyceride,3%hydrogenated lecithin,2%ascorbyl tetraisopalmitate,1%pentylene glycol,and 0.5%1,2-hexanediol),and measured its particle size and zeta potential using laser diffraction particle analyzer and evaluated its efficacy against pure pterostilbene using in vitro melanocyte and in vivo zebrafish embryo models.PUFECBENE®exhibited uniform aqueous dispersion with a hydrodynamic diameter of 121.72±1.68 nm,a zeta potential of−65.1±0.15 mV,and a transmittance of 80.64±0.062%.The cytotoxicity assay showed that PUFECBENE®exhibited no cytotoxicity in melanocytes at concentrations≤1.25μg/mL,while pure pterostilbene was non-toxic up to 3.13μg/mL.Melanocyte assays demonstrated that 1.25μg/mL PUFECBENE®reduced melanin synthesis by 57.05±4.09%,comparable to pure pterostilbene(57.58±0.76%at 3.13μg/mL),and immunostaining revealed a 3.36-fold increase in LC3-Ⅱ(autophagosome marker)and significant LC3-Ⅱ/Pmel17(Pmel17 is a melanosome-specific protein)co-localization,indicating autophagy-mediated melanosome degradation.Zebrafish embryos treated with PUFECBENE®(0.5μg/mL)showed 99.30±3.44%melanogenesis inhibition,outperforming pure pterostilbene(8.95±7.19%at 0.5μg/mL).PUFECBENE®exhibited a 50-fold enhanced in vivo potency relative to pure pterostilbene while retaining comparable in vitro efficacy,highlighting the critical role of bioavailability optimization and in vivo analysis in translating in vitro findings to formulation applications.This highlights the success of microencapsulation in enhancing bioavailability and depigmentation activity,positioning PUFECBENE®as a promising candidate for advanced cosmetic formulations targeting hyperpigmentation. 展开更多
关键词 Melanin Microencapsulation Autophagy Pterostilbene
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Anti-aging Efficacy of a 0.1%Pterostilbene Skincare Emulsion:A 28-Day Clinical Trial 认领 引用
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作者 Zongxiao Cen Zhiyuan Chen +3 位作者 Ding Wang Yuqin Zuo Junyuan Chen Xueping Chen 《Journal of Dermatologic Science and Cosmetic Technology》 2025年第2期17-26,共10页
Pterostilbene is gaining recognition as a promising ingredient in skincare for its anti-aging benefits.This study assessed the clinical effectiveness of a 0.1%pterostilbene-containing skincare emulsion against a contr... Pterostilbene is gaining recognition as a promising ingredient in skincare for its anti-aging benefits.This study assessed the clinical effectiveness of a 0.1%pterostilbene-containing skincare emulsion against a control emulsion over 28 days with 31 participants.A double-blind,split-face design was used,comparing the left and right sides of the face and preand post-treatment conditions.Efficacy was measured using advanced tools,including the Skin Elasticity Tester CutometerR dual MPA580,the facial imaging and analysis system VISIAR-CR,the skin microstructure 3D imaging system PRIMOSCR,and the in vivo two-photon microscopy imaging system SUPERVISION-780,along with subjects’selfassessment after 14 and 28 days of continual use.Results demonstrated that the 0.1%pterostilbene emulsion significantly enhanced skin elasticity R2 and firmness F4,reduced the area and volume of forehead and undern-eye wrinkles,decreased the percentage of Crow’s feet wrinkles area,improved the aging index ELCOR,and increased the thickness of the epidermis layer,collagen intensity,elastic fiber fluorescence,and the standardized area of the epidermal-dermal junction(DEJ).Compared to the control emulsion,the pterostilbene emulsion showed statistically significant(p<0.05)improvements in these metrics,as well as aging index SAIID and skin pore size.All subjects expressed higher satisfaction with the pterostilbene emulsion,highlighting its superior skincare benefits.The findings emphasize the potent anti-aging efficacy of pterostilbene,reinforcing its value as an active ingredient in cosmetics formulations. 展开更多
关键词 Antiaging Clinical study Pterostilbene Wrinkles
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科尔沁沙地农田面积变化特征与沙尘源治理对策 认领 引用 被引量：3
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作者 王宁 丛安琪 +1 位作者 陈雪萍 刘新平 《中国沙漠》 CSCD 北大核心 2025年第4期253-261,共9页
研究区域土地利用变化特征并寻求其合理利用的途径,对于区域可持续发展具有重要意义。以科尔沁沙地农田面积变化为切入点,梳理了该区域农田面积的历史变迁与现状,重点探讨了农田面积变化对农田风蚀的影响,提出了农田沙尘源治理的有效途... 研究区域土地利用变化特征并寻求其合理利用的途径,对于区域可持续发展具有重要意义。以科尔沁沙地农田面积变化为切入点,梳理了该区域农田面积的历史变迁与现状,重点探讨了农田面积变化对农田风蚀的影响,提出了农田沙尘源治理的有效途径和方法。建议加强尘源地农田监控与预警、加强农田防风体系建设,对农田防护林进行全方位建设和管理,从而充分发挥其重要防风屏障功能;通过实行保护性耕作、冬季作物覆盖种植等措施减弱风蚀,提高农田土层保护能力;加强农田土壤水分和养分综合管理,大力推广节水灌溉和土壤有机培肥等措施,实现水肥资源的高效利用和土壤质量的综合提升;针对不同旱作和灌溉农田进行分类实施治理与调整管理,提高治理综合效率。 展开更多
关键词 科尔沁沙地 土地利用 农田面积 沙尘源 土壤质量
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科尔沁沙地植物适应性及沙丘植被格局 认领 引用 被引量：2
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作者 赵学勇 王海军 +4 位作者 陈雪萍 卢建男 杨小菊 赵思腾 王瑞雄 《中国沙漠》 CSCD 北大核心 2025年第4期8-15,共8页
植物适应性指植物通过对水分与营养元素获取、调节、生长和功能维持等过程获得的在多时空尺度上的特征优化及生存能力,是沙地植被恢复与土地退化防治的理论基础。然而基于沙地沉积物、植物耗水规律、地下水埋深变化与沙地植物群落关系... 植物适应性指植物通过对水分与营养元素获取、调节、生长和功能维持等过程获得的在多时空尺度上的特征优化及生存能力,是沙地植被恢复与土地退化防治的理论基础。然而基于沙地沉积物、植物耗水规律、地下水埋深变化与沙地植物群落关系的植物适应性研究不足。针对这一问题,对科尔沁沙地地质沉积特征、不同类型沙丘植被特征及其与地下水埋深的关系进行了综述性研究与分析。结果显示:科尔沁沙地沙质沉积深度沿河及由山地到平原逐步增加,沙地中部沉积深度达到200 m;不同类型沙丘间植物群落特征差异显著,流动沙丘植物组成变化最为明显;地下水埋深增加影响沙地植物分布格局与组成特征,沙丘起伏影响植物与地下水埋深变化的关系。沙地主要植物耗水量为300~450 mm。基于植物耗水量与调查结果,科尔沁沙地的合理造林密度应为225~375株·hm-2。 展开更多
关键词 植物适应性 持续恢复 稳定植被 协同关系 科尔沁沙地
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内蒙古奈曼旗1985—2020年地下水埋深时空变化特征 认领 引用 被引量：2
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作者 陈雪萍 赵学勇 +3 位作者 庄海艳 乔宇来 于红梅 张晶 《中国沙漠》 CSCD 北大核心 2025年第4期166-175,共10页
奈曼旗是中国北方半干旱农牧交错带重要的粮食生产基地,但近年来水资源供需矛盾日益突出。随着农业种植面积不断扩大,该旗地下水埋深持续增加,水资源短缺问题愈发严峻。本研究基于奈曼旗25眼地下水监测井长期观测数据,结合土地利用类型... 奈曼旗是中国北方半干旱农牧交错带重要的粮食生产基地,但近年来水资源供需矛盾日益突出。随着农业种植面积不断扩大,该旗地下水埋深持续增加,水资源短缺问题愈发严峻。本研究基于奈曼旗25眼地下水监测井长期观测数据,结合土地利用类型和海拔特征将研究区划为3个分区——Ⅰ区北部农田区、Ⅱ区中部沙地区和Ⅲ区南部山地区,采用克里金插值法分析了1985—2020年地下水埋深时空演变特征及其影响因素。结果表明:(1)研究时段内奈曼旗地下水埋深整体呈增加趋势,1995—1999年因降水增加有所抬升。(2)各分区年内地下水埋深变化特征因地下水动态类型各有差异;年际特征上,Ⅰ区增速(0.22 m·a-1)显著高于Ⅱ区和Ⅲ区(均为0.09 m·a-1),大沁他拉镇增幅最突出。(3)旗域内耕地-草地-裸地-建设用地之间的转化最为显著,特别是Ⅰ区耕地扩张和Ⅱ区城镇发展对地下水埋深变化具有决定性影响。(4)2000年前,各分区地下水埋深受气象因素交互作用显著;2000年后,Ⅰ区灌溉面积与各驱动因子交互作用全面增强;Ⅱ区维持以气温和蒸发为主导的交互模式;Ⅲ区降水量和灌溉面积与各驱动因子交互作用逐渐增强。 展开更多
关键词 地下水埋深 时空分布 土地利用 影响因素 奈曼旗
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Oxidative stress, mitochondrial damage and neurodegenerative diseases 认领 引用 被引量：48
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作者 Chunyan Guo Li Sun +1 位作者 Xueping Chen Danshen Zhang 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第21期2003-2014,共12页
Oxidative stress and mitochondrial damage have been implicated in the pathogenesis of several neurodegenerative diseases, including Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis. Oxidati... Oxidative stress and mitochondrial damage have been implicated in the pathogenesis of several neurodegenerative diseases, including Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis. Oxidative stress is characterized by the overproduction of reactive oxygen species, which can induce mitochondrial DNA mutations, damage the mitochondrial respiratory chain, alter membrane permeability, and influence Ca2＋ homeostasis and mitochondrial defense systems. All these changes are implicated in the development of these neurodegenerative diseases, mediating or amplifying neuronal dysfunction and triggering neurodegeneration. This paper summarizes the contribution of oxidative stress and mitochondrial damage to the onset of neurodegenerative diseases and discusses strategies to modify mitochondrial dysfunction that may be attractive therapeutic interventions for the treatment of various neurodegenerative diseases. 展开更多
关键词 neural regeneration neurodegenerative diseases Alzheimer’s disease Parkinson’s disease amyotrophic lateral sclerosis oxidative stress reactive oxygen species mitochondrial damage respiratory chain grants-supported paper neuroregeneration
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Exogenous melatonin improves the chilling tolerance and preharvest fruit shelf life in eggplant by affecting ROS-and senescence-related processes 认领 引用 被引量：20
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作者 Lijun Song Zhuo Tan +5 位作者 Weiwei Zhang Qiang Li Zhaoxiang Jiang Shuxing Shen Shuangxia Luo Xueping Chen 《Horticultural Plant Journal》 SCIE CAS CSCD 2023年第3期523-540,共18页
Low temperature is the most common abiotic stress factors during the eggplant cultivation in solar greenhouses.Melatonin plays important roles in plant resistance to low temperature.However,the role of melatonin in re... Low temperature is the most common abiotic stress factors during the eggplant cultivation in solar greenhouses.Melatonin plays important roles in plant resistance to low temperature.However,the role of melatonin in regulating chilling tolerance and extending the preharvest shelf life of eggplant fruits is still unknown.In this study,we investigated the effects of exogenous melatonin on eggplant plants and fruits in response to low temperature.Under simulated low-temperature conditions,exogenous melatonin significantly relieved the chilling symptoms of seedlings by reducing reactive oxygen species (ROS) and malondialdehyde (MDA) levels and relative leakage rates.These reductions were caused by higher superoxide dismutase (SOD) and catalase (CAT) activities and increased endogenous polyamine and melatonin levels compared with those in untreated seedlings.Notably,the expression levels of SOD,CAT1/2,and polyamine synthesis genes (ADC and ODC) were also increased by 100μmol·L~(-1)melatonin,as well as those of genes involved in melatonin synthesis (TDC,T5H,SNAT,ASMT,and COMT) and cold regulation (COR1,CBFa/b,and ZAT2/6/12).To further investigate the effects of melatonin on eggplant leaves and fruits under natural low temperature conditions,100μmol·L~(-1)melatonin was sprayed on the functional leaves at three days before commodity maturation.Melatonin significantly alleviated chilling injury in the leaves and pericarp and extended the preharvest shelf life of the fruit by increasing the expression of COR1,CBF,ZAT2/6/12,and API5 and decreasing the expression of senescence-related genes (NCED1/2 and SAG12).Therefore,100μmol·L~(-1)melatonin improved chilling tolerance and fruit shelf life by upregulating ZAT2/6/12 to affect ROS-and senescence-related processes,which provides a reference for alleviating cold stress and extending the preharvest fruit shelf life in eggplant. 展开更多
关键词 Eggplant Melatonin Chilling tolerance Cold regulation Reactive oxygen species Preharvest fruit shelf life
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Oxidative stress in neurodegenerative diseases 认领 引用 被引量：26
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作者 Xueping Chen Chunyan Guo Jiming Kong 《Neural Regeneration Research》 SCIE CAS CSCD 2012年第5期376-385,共10页
Reactive oxygen species are constantly produced in aerobic organisms as by-products of normal oxygen metabolism and include free radicals such as superoxide anion （02-） and hydroxyl radical （OH-）, and non-radical ... Reactive oxygen species are constantly produced in aerobic organisms as by-products of normal oxygen metabolism and include free radicals such as superoxide anion （02-） and hydroxyl radical （OH-）, and non-radical hydrogen peroxide （H202）. The mitochondrial respiratory chain and enzymatic reactions by various enzymes are endogenous sources of reactive oxygen species. Exogenous reactive oxygen species -inducing stressors include ionizing radiation, ultraviolet light, and divergent oxidizing chemicals. At low concentrations, reactive oxygen species serve as an important second messenger in cell signaling; however, at higher concentrations and long-term exposure, reactive oxygen species can damage cellular macromolecules such as DNA, proteins, and lipids, which leads to necrotic and apoptotic cell death. Oxidative stress is a condition of imbalance between reactive oxygen species formation and cellular antioxidant capacity due to enhanced ROS generation and/or dysfunction of the antioxidant system. Biochemical alterations in these macromolecular components can lead to various pathological conditions and human diseases especially neurodegenerative diseases. Neurodegenerative diseases are morphologically featured by progressive cell loss in specific vulnerable neuronal cells, often associated with cytoskeletal protein aggregates forming inclusions in neurons and/or glial cells. Deposition of abnormal aggregated proteins and disruption of metal ions homeostasis are highly associated with oxidative stress. The main aim of this review is to present as much detailed information as possible that is available on various neurodegenerative disorders and their connection with oxidative stress. A variety of therapeutic strategies designed to address these pathological processes are also described. For the future therapeutic direction, one specific pathway that involves the transcription factor nuclear factor erythroid 2-related factor 2 is receiving considerable attention. 展开更多
关键词 oxidative stress neurodegenerative diseases reactive oxygen species therapy reviews
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Molecular and genetic regulations of fleshy fruit shape and lessons from Arabidopsis and rice 认领 引用 被引量：8
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作者 Qiang Li Shuangxia Luo +9 位作者 Liying Zhang Qian Feng Lijun Song Manoj Sapkota Shuxin Xuan Yanhua Wang Jianjun Zhao Esther van der Knaap Xueping Chen Shuxing Shen 《Horticulture Research》 SCIE CSCD 2023年第7期176-191,共16页
Fleshy fruit shape is an important external quality trait influencing the usage of fruits and consumer preference.Thus,modification of fruit shape has become one of the major objectives for crop improvement.However,th... Fleshy fruit shape is an important external quality trait influencing the usage of fruits and consumer preference.Thus,modification of fruit shape has become one of the major objectives for crop improvement.However,the underlying mechanisms of fruit shape regulation are poorly understood.In this review we summarize recent progress in the genetic basis of fleshy fruit shape regulation using tomato,cucumber,and peach as examples.Comparative analyses suggest that the OFP-TRM(OVATE Family Protein-TONNEAU1 Recruiting Motif)and IQD(IQ67 domain)pathways are probably conserved in regulating fruit shape by primarily modulating cell division patterns across fleshy fruit species.Interestingly,cucumber homologs of FRUITFULL(FUL1),CRABS CLAW(CRC)and 1-aminocyclopropane-1-carboxylate synthase 2(ACS2)were found to regulate fruit elongation.We also outline the recent progress in fruit shape regulation mediated by OFP-TRM and IQD pathways in Arabidopsis and rice,and propose that the OFP-TRM pathway and IQD pathway coordinate regulate fruit shape through integration of phytohormones,including brassinosteroids,gibberellic acids,and auxin,and microtubule organization.In addition,functional redundancy and divergence of the members of each of the OFP,TRM,and IQD families are also shown.This review provides a general overview of current knowledge in fruit shape regulation and discusses the possible mechanisms that need to be addressed in future studies. 展开更多
关键词 Arabidopsis Molecular probably
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Gene characterization and molecular pathway analysis of reverse thermosensitive genic male sterility in eggplant(Solanum melongena L.) 认领 引用 被引量：7
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作者 Bing Li Xueping Chen +7 位作者 Yanrong Wu Aixia Gu Jingjing Zhang Shuangxia Luo Xiurui Gao Jianjun Zhao Xiuqing Pan Shuxing Shen 《Horticulture Research》 SCIE 2019年第1期221-236,共16页
The naturally occurring mutant eggplant line 05ms was identified with reverse thermosensitive genic male sterility(rTGMS),but its temperature-responsive fertility mechanisms remain largely unknown.Here,we studied the ... The naturally occurring mutant eggplant line 05ms was identified with reverse thermosensitive genic male sterility(rTGMS),but its temperature-responsive fertility mechanisms remain largely unknown.Here,we studied the flower morphology,anther cellular structure,and genome-wide gene expression of this rTGMS line.Candidate genes for thermosensitive male sterility during the microspore development of 05ms and the temperature-insensitive line S63 under low-temperature(LT)and high-temperature(HT)conditions were identified.Under LT,tapetum cells were vacuolated and had delayed disintegration in 05ms.RNA-seq analysis indicated that DEGs were enriched in the KEGG pathways‘plant hormone signal transduction’,‘starch and sucrose metabolism’,and‘phenylpropanoid biosynthesis’.We identified two genes,4CLL1(Sme2.5_00368.1_g00010.1)and CKI1(Sme2.5_10056.1_g00002.1),which could potentially regulate eggplant anther development and may be candidate genes for rTGMS.Finally,we propose a working model of anther abortion for rTGMS in eggplant.CKI1 responds to LT stress and causes expression changes in genes related to anther development,such as 4CLL1,and the cellular structure of the tapetum becomes abnormal,causing male sterility.The findings of this study explain the underlying molecular mechanisms of male sterility in eggplant rTGMS lines. 展开更多
关键词 metabolism analysis sterility
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Comparative transcriptome analysis reveals defense responses against soft rot in Chinese cabbage 认领 引用 被引量：7
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作者 Mengyang Liu Fang Wu +6 位作者 Shan Wang Yin Lu Xueping Chen Yanhua Wang Aixia Gu Jianjun Zhao Shuxing Shen 《Horticulture Research》 SCIE 2019年第1期888-905,共18页
Pectobacterium carotovorum ssp.carotovorum(Pcc)is a necrotrophic bacterial species that causes soft rot disease in Chinese cabbage.In this study,plants harboring the resistant mutant sr gene,which confers resistance a... Pectobacterium carotovorum ssp.carotovorum(Pcc)is a necrotrophic bacterial species that causes soft rot disease in Chinese cabbage.In this study,plants harboring the resistant mutant sr gene,which confers resistance against Pcc,were screened from an 800 M2 population mutated by ethyl methane sulfonate(EMS)and scored in vitro and in vivo for lesion size.The transcript profiles showed~512 differentially expressed genes(DEGs)between sr and WT plants occurring between 6 and 12 h postinoculation(hpi),which corresponded to the important defense regulation period(resistance)to Pcc in Chinese cabbage.The downstream defense genes(CPK,CML,RBOH MPK3,and MPK4)of pathogen pattern-triggered immunity(PTI)were strongly activated during infection at 12 hpi in resistant mutant sr;PTI appears to be central to plant defense against Pcc via recognition by three putative pattern recognition receptors(PRRs;BrLYM1-BrCERK1,BrBKK1/SERK4-PEPR1,BrWAKs).Pcc triggered the upregulation of the jasmonic acid(JA)and ethylene(ET)biosynthesis genes in mutant sr,but auxins and other hormones may have affected some negative signals.Endogenous hormones(auxins,JAs,and SA),as well as exogenous auxins(MEJA and BTH),were also verified as functioning in the immune system.Concurrently,the expression of glucosinolate and lignin biosynthesis genes was increased at 12 hpi in resistant mutant sr,and the accumulation of glucosinolate and lignin also indicated that these genes have a functional defensive role against Pcc.Our study provides valuable information and elucidates the resistance mechanism of Chinese cabbage against Pcc infection. 展开更多
关键词 immunity pattern harbor
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Genetic analysis of the “head top shape” quality trait of Chinese cabbage and its association with rosette leaf variation 认领 引用 被引量：4
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作者 Xiaoxue Sun Ying Gao +12 位作者 Yin Lu Xiaomeng Zhang Shuangxia Luo Xing Li Mengyang Liu Daling Feng Aixia Gu Xueping Chen Shuxin Xuan Yanhua Wang Shuxing Shen Guusje Bonnema Jianjun Zhao 《Horticulture Research》 SCIE CSCD 2021年第1期1374-1385,共12页
The agricultural and consumer quality of Chinese cabbage is determined by its shape.The shape is defined by the folding of the heading leaves,which defines the head top shape(HTS).The overlapping HTS,in which the head... The agricultural and consumer quality of Chinese cabbage is determined by its shape.The shape is defined by the folding of the heading leaves,which defines the head top shape(HTS).The overlapping HTS,in which the heading leaves curve inward and overlap at the top,is the shape preferred by consumers.To understand the genetic regulation of HTS,we generated a large segregating F 2 population from a cross between pak choi and Chinese cabbage,with phenotypes ranging from nonheading to heading with either outward curving or inward curving overlapping heading leaves.HTS was correlated with plant height,outerosette leaf length,and petiole length.A high-density genetic map was constructed.Quantitative trait locus(QTL)analysis resulted in the identification of 22 QTLs for leafy head-related traits,which included five HTS QTLs.Bulked segregant analysis(BSA)was used to confirm HTS QTLs and identify candidate genes based on informative single-nucleotide polymorphisms.Interestingly,the HTS QTLs colocalized with QTLs for plant height,outerosette leaf,and petiole length,consistent with the observed phenotypic correlations.Combined QTL analysis and BSA laid a foundation for molecular marker-assisted breeding of Chinese cabbage HTS and directions for further research on the genetic regulation of this trait. 展开更多
关键词 heading trait breeding
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Fast tracking alien gene discovery by molecular markers in a late flowering Chinese cabbage-cabbage translocation line‘AT7–4' 认领 引用 被引量：2
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作者 Hui Li Aixia Gu +12 位作者 Daling Feng Na Li Rui Yang Xinpei Zhang Shuangxia Luo Umer Karamat Qianyun Wang Shuxin Xuan Xueping Chen Yin Lu Yanhua Wang Shuxing Shen Jianjun Zhao 《Horticultural Plant Journal》 SCIE CAS CSCD 2023年第1期89-97,共9页
Flowering time is an important agronomic trait of Chinese cabbage with late flowering being a primary breeding objective.In our previous work,we obtained Chinese cabbage-cabbage translocation lines that contained seve... Flowering time is an important agronomic trait of Chinese cabbage with late flowering being a primary breeding objective.In our previous work,we obtained Chinese cabbage-cabbage translocation lines that contained several beneficial cabbage genes.Cabbage-specific molecular markers show that these genes were coming from chromosome C01 of cabbage.In this study,we investigated the inheritance of flowering time in a couple of translocation lines and analyzed the transmission rate of molecular markers in the offspring.Consequently,we obtained the late flowering Chinese cabbage-cabbage translocation line‘AT7–4’in which the flowering time was later than that of‘85–1’by about 7 days under 4-week vernalization.Based on previous studies of the genomes of Chinese cabbage and cabbage,we located the cabbage-specific molecular markers that were closely linked at the top of the chromosome A01 in the F2mapping population generated by self-crossing F1s derived from a cross between the translocation line‘AT7–4’and Chinese cabbage‘14–36’.Five flowering-related genes in the alien fragment were found by functional annotation and their molecular markers were developed.This study lays the foundation for the future improvement of Chinese cabbage varieties using A-C translocation lines. 展开更多
关键词 Chinese cabbage Translocation line Alien gene Molecular marker Late flowering
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Role of DYT1 gene in early-onset primary torsion dystonia 认领 引用 被引量：1
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作者 Xiaohui Hu Xueping Chen +1 位作者 Rui Huang Huifang Shang 《Neural Regeneration Research》 SCIE CAS 2010年第18期1429-1434,共6页
Mutation of the DYT1 gene has been reported to cause early-onset primary torsion dystonia(DYT1)Due to DYT1 gene mutation,defective wild torsinA and the accumulation of mutant torsinA(GAG-deleted DYT1 gene encoded the ... Mutation of the DYT1 gene has been reported to cause early-onset primary torsion dystonia(DYT1)Due to DYT1 gene mutation,defective wild torsinA and the accumulation of mutant torsinA(GAG-deleted DYT1 gene encoded the mutant torsinA,torsinA&E)play an important role in DYT1 pathogenesis.Intracellular inclusion bodies are formed,and dopamine transport and release are disturbed by interfering functions of endoplasmic reticulum,nuclear membrane,and cytoskeleton of neural cells,resulting in DYT1 onset.Small interfering RNA could serve as a potential therapy for DYT1.However,the exact function of wild torsinA and the pathological effects of torsinAAE require further studies. 展开更多
关键词 early-onset primary torsion dystonia torsinA DYT1 RNA interference
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Genome-wide development and utilization of Simple Sequence Repeats in Chinese cabbage(Brassica rapa L.ssp.pekinensis) 认领 引用 被引量：1
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作者 Ying Gao Na Li +12 位作者 Xiaoguang Li Yin Lu Daling Feng Xiaomeng Zhang Aixia Gu Yunjia Ge Javaria Tabusam Mengyang Liu Yanhua Wang Xueping Chen Shuxing Shen Shuangxia Luo Jianjun Zhao 《Vegetable Research》 2022年第1期81-87,共7页
With the complementation of whole-genome sequencing of Chinese cabbage,it is necessary to develop genome-wide Simple Sequence Repeat(SSR)markers and analyse their characteristics,which will bring a revolution in the m... With the complementation of whole-genome sequencing of Chinese cabbage,it is necessary to develop genome-wide Simple Sequence Repeat(SSR)markers and analyse their characteristics,which will bring a revolution in the molecular marker-assisted breeding of Chinese cabbage.In this study distribution and characteristics of SSR loci in the genome of Chinese cabbage'Chiifu'was analyzed.Finally,a total of 173,892 SSR markers that occurred in 10 chromosomes were identified,and Perfect Repeat Motifs(P type)which is a main form of SSR loci with a mean distance of 1.89 kb/SSR was identified.Among them,mono-,di-,and tri-nucleotide repeat motifs were equally distributed across chromosomes compared with tetra-,penta-,and hexa-nucleotide repeat motifs.The largest proportion of SSR loci consisted of mono-and dinucleotide repeat motifs,which accounted for 93.04%of the total SSR loci.A/T and AT/TA were the most abundant motifs,accounting for 97.61%and 64.05%of the mono-and dinucleotide repeats,respectively.Furthermore,200 SSR markers were used for the amplification of 12 Chinese cabbage germplasm,in which 30 were highly polymorphic.These SSR markers were further used for the association analyses of flowering time of 190 Chinese cabbage accessions.The results revealed that 9 and 10 alleles were associated with bolting and flowering time,respectivley.The results show that development of SSR markers is feasible and useful in marker assisted selection of Chinese cabbage. 展开更多
关键词 breeding Sequence characteristics
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Lipid biomarker composition in surface sediments from the Carlsberg Ridge near the Tianxiu Hydrothermal Field 认领 引用
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作者 Shengyi Mao Hongxiang Guan +5 位作者 Lihua Liu Xiqiu Han Xueping Chen Juan Yu Yongge Sun Yejian Wang 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2021年第8期53-64,共12页
Hydrothermal venting has a profound effect on the chemical and biological properties of local and distal seawater and sediments. In this study, lipid biomarkers were analyzed to examine the potential influence of hydr... Hydrothermal venting has a profound effect on the chemical and biological properties of local and distal seawater and sediments. In this study, lipid biomarkers were analyzed to examine the potential influence of hydrothermal activity on the fate of organic matter(OM) in surface sediments around Tianxiu Hydrothermal Field in the Carlsberg Ridge(CR), Northwest Indian Ocean. By comparing the biomarker distributions of the samples with that of other typical hydrothermal sediments in the mid ocean ridge, it is shown that the location of the samples is not affected by the hydrothermal activity. The relatively low abundances of terrestrial n-alkyl lipids and riverine1,15-C32 diol suggested a minor contribution of terrigenous OM to the study area. The bacteria contributed predominantly to sedimentary marine OM;however, other marine source organisms, e.g., eukaryotes(i.e.,phytoplankton and fungi) could not be completely neglected. The marine-originated biomarkers showed significantly variable distributions between the two sediments, suggesting different dynamic physical and biogeochemical processes controlling the fate of marine OM. This study identified various diagnostic biomarkers(5,5-diethyl alkanes, diols and β-OH FAs), which may have significant environmental implications for future works in this region. 展开更多
关键词 Carlsberg Ridge Tianxiu Hydrothermal Field surface sediments biomarkers
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Genetic classification and molecular mechanisms of primary dystonia 认领 引用
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作者 Xueping Chen Huifang Shang Zuming Luo 《Neural Regeneration Research》 SCIE CAS 2008年第3期296-300,共5页
BACKGROUND:Primary dystonia is a heterogeneous disease,with a complex genetic basis.In previous studies,primary dystonia was classified according to age of onset,involved regions,and other clinical characteristics.Wit... BACKGROUND:Primary dystonia is a heterogeneous disease,with a complex genetic basis.In previous studies,primary dystonia was classified according to age of onset,involved regions,and other clinical characteristics.With the development of molecular genetics,new virulence genes and sites have been discovered.Therefore,there is a gradual understanding of the various forms of dystonia,based on new viewpoints.There are 15 subtypes of dystonia,based on the molecular level,i.e.,DYT1 to DYT15.OBJECTIVE:To analyze the genetic development of dystonia in detail,and to further investigate molecular mechanisms of dystonia.RETRIEVAL STRATEGY:A computer-based online search was conducted in PubMed for English language publications containing the keywords"dystonia and genetic"from January 1980 to March 2007.There were 105 articles in total.Inclusion criteria:①the contents of the articles should closely address genetic classification and molecular mechanisms of primary dystonia;②the articles published in recent years or in high-impact journals took preference.Exclusion criteria:duplicated articles.LITERATURE EVALUATION:The selected articles were on genetic classification and molecular genetics mechanism of primary dystonia.Of those,27 were basic or clinical studies.DATA SYNTHESlS:①Dystonia is a heterogeneous disease,with a complex genetic basis.According to the classification of the Human Genome Organization,there are 15 dystonia subtypes,based on genetics,i.e.,DYT1-DYT15,including primary dystonia,dystonia plus syndrome,degeneration plus dystonia,and paroxysmal dyskinesia plus dystonia.②To date,the chromosomes of 13 subtypes have been localized;however,DYT2 and DYT4 remain unclear.Six subtypes have been located within virulence genes.Specifically,torsinA gene expression results in the DYTI genotype;autosomal dominant GTP cyclohydrolase 1 gene expression and recessive tyrosine hydroxylase expression result in the DYT5 genotype,respectively;the epsilon-sarcoglycan gene is involved in DYT11;Na^+/K^+-ATP enzymeα3 chain gene in DYT12;TATA-conjugated protein-associated factor 1 gene in DYT3;and myofibril regulatory factor gene in DYTS.③Different types of dystonia exhibit various clinical characteristics and specific clinical manifestations.④Many elements regarding the molecular mechanism of dystonia have been determined.However,many components remain poorly understood.For example,detailed pathogenesis remains unclear.Various forms of dystonia exhibit similar problems.Moreover,a single form of dystonia may be a result of two or more different chromosomal mutations.In addition,more studies are needed to fully understand chromosome apposition and virulence genes involved in dystonia.CONCLUSION:The discovery of virulence genes and localizations of newly classified forms of dystonia are beneficial to further understanding the molecular mechanisms of dystonia. 展开更多
关键词 primary dystonia molecular genetics review literature
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Mapping and identification of genes responsible for lessphotosensitive fruit coloration in eggplant 认领 引用
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作者 Lei Luo Yinan Niu +9 位作者 Qiang Li Linfeng Xia Chunyang Wang Shuangxia Luo Na Li Shuxin Xuan Yanhua Wang Shuxing Shen Jianjun Zhao Xueping Chen 《Vegetable Research》 2023年第1期1-8,共8页
In eggplant(Solanum melongena.L),low light during cultivation often hinders proper pigmentation of fruit.While some varieties exhibit less susceptibility to low light for eggplant coloration,however,the genetic basis ... In eggplant(Solanum melongena.L),low light during cultivation often hinders proper pigmentation of fruit.While some varieties exhibit less susceptibility to low light for eggplant coloration,however,the genetic basis of such less-photosensitive fruit coloration remains unknown.In this study,we characterized a less-photosensitive eggplant cultivar‘609'.Under bagging conditions,fruits of‘609'exhibited purple coloration,albeit lighter than fruits grown under natural conditions.Genetic analysis showed that the less-photosensitive trait was controlled by a single dominant gene,designated SmLP.Based on BSA and genetic recombination analyses,SmLP was mapped to the 7.4−12.5 Mb region on chromosome 10.Within this genetic region,six genes with non-synonymous mutation and seven genes potentially involved in anthocyanin biosynthesis or light signal transduction were identified.Further RT-qPCR analysis revealed that only three out of these genes were differentially expressed in eggplant peel tissues.The three genes EGP21875,EGP21864 and EGP21911,encoding MYB domain protein 113,phototropic-responsive NPH3 family protein,and protein with unknown function,respectively,were considered as the putative genes associated with less-photosensitive anthocyanin biosynthesis in eggplant fruits.These results would provide great help in casual gene identification for less-photosensitive trait and promote an understanding of molecular mechanisms underlying less-and non-photosensitive coloration in eggplant. 展开更多
关键词 eggplant fruit coloration anthocyanin biosynthesis SMLP light signal transduction photosensitive genetic basis chromosome
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