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肌醇六磷酸和大豆提取物复配牛初乳增强免疫功能研究 认领 引用 被引量：1
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作者 张露勇 张励 +3 位作者 马利波 郑济凡 李波 刘婷 《解放军药学学报》 2025年第2期123-129,共7页
目的 比较研究牛初乳基础配方(基础型,BF)和基础配方复配肌醇六磷酸(优化型,OF)的2种保健食品对免疫力的增强功能。方法 雌性SPF级KM小鼠400只随机分为4个剂量组,用于开展6个实验。BF剂量组设置为:354、118、59 mg·kg-1;OF剂量... 目的 比较研究牛初乳基础配方(基础型,BF)和基础配方复配肌醇六磷酸(优化型,OF)的2种保健食品对免疫力的增强功能。方法 雌性SPF级KM小鼠400只随机分为4个剂量组,用于开展6个实验。BF剂量组设置为:354、118、59 mg·kg-1;OF剂量组设置为:600、200、100 mg·kg-1。灌胃给予每日1次,连续30 d。结果 OF 600 mg·kg-1显著增强小鼠的耳郭肿胀,三个剂量组的小鼠脾淋巴细胞增殖能力均显著增强,细胞免疫实验结果为阳性;600 mg·kg-1 OF升高小鼠血清溶血素抗体水平值,三个剂量组均显著升高抗体生成全脾溶血空斑数,体液免疫实验结果为阳性;虽然600 mg·kg-1OF增加腹腔吞噬鸡红细胞指数,但三个剂量组小鼠碳廓清均为阴性,因此单核-巨噬细胞实验为阴性;仅200 mg·kg-1 OF组升高小鼠NK细胞活性率,因此NK细胞活性实验结果阴性。354 mg·kg-1 BF增强小鼠耳郭肿胀,但脾淋巴细胞转换三剂量组均为阴性,因此细胞免疫实验结果为阴性;354、59 mg·kg-1 BF升高小鼠血清溶血素抗体水平值,虽然全脾溶血空斑计数三剂量组均为阴性,但体液免疫实验结果仍为阳性;虽然59 mg·kg-1 BF增加腹腔吞噬鸡红细胞指数,但小鼠碳廓清三剂量组均为阴性,因此单核-巨噬细胞实验为阴性;NK细胞活性实验三剂量组均为阴性。结论BF增强免疫力功能为阴性。OF增强免疫力功能为阳性,值得深入研究和开发。 展开更多
关键词 肌醇六磷酸 大豆提取物 牛初乳 免疫调节 小鼠
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特殊食品验证评价技术机构备案工作要点解析 认领 引用
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作者 张露勇 李波 +3 位作者 郑济凡 崔生辉 孙磊 刘师卜 《解放军药学学报》 2025年第5期494-498,共5页
目的为提高特殊食品验证评价技术机构备案工作效率、贯彻加强事后监管的总体思想,提出规范执行、备案合理化建议。方法结合《检验检测机构资质认定评审准则》,解析《特殊食品验证评价技术机构工作规范》。对开展备案的背景和目的、备案... 目的为提高特殊食品验证评价技术机构备案工作效率、贯彻加强事后监管的总体思想,提出规范执行、备案合理化建议。方法结合《检验检测机构资质认定评审准则》,解析《特殊食品验证评价技术机构工作规范》。对开展备案的背景和目的、备案系统的定位和勘验流程、机构备案的内容要求、备案时应注意的事项4个方面解读。重点对管理原则、工作团队、主要仪器、设施设备和环境场所、质量管理体系运行情况、档案管理、风险管理等内容进行解析。采取释义和举例结合方式说明撰写备案材料的侧重点。最后再针对既往备案时机构材料准备中常见的问题,分析指出注意事项。结果机构应通过加强学习理解、加强标准查新与备案更新,持续完善质量管理体系,有利于顺利通过备案。结论特殊食品验证评价技术机构备案系统是《特殊食品验证评价技术机构工作规范》的具体实施,有利于提高监管的透明度、解决企业与技术机构之间的信息不对称问题。通过备案工作要点解析,可提高机构备案效率。 展开更多
关键词 特殊食品 特殊食品检验检测机构备案
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Organic anion transporter 1 and 3 contribute to traditional Chinese medicine-induced nephrotoxicity 认领 引用 被引量：19
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作者 SHEN Qing-Qing WANG Jing-Jing +4 位作者 ROY Debmalya SUN Li-Xin JIANG Zhen-Zhou ZHANG Lu-Yong HUANG Xin 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2020年第3期196-205,共10页
With the internationally growing popularity of traditional Chinese medicine(TCM), TCM-induced nephropathy has attracted public attention. Minimizing this toxicity is an important issue for future research. Typical nep... With the internationally growing popularity of traditional Chinese medicine(TCM), TCM-induced nephropathy has attracted public attention. Minimizing this toxicity is an important issue for future research. Typical nephrotoxic TCM drugs such as Aristolochic acid, Tripterygium wilfordii Hook. f, Rheum officinale Baill, and cinnabar mainly damage renal proximal tubules or cause interstitial nephritis. Transporters in renal proximal tubule are believed to be critical in the disposition of xenobiotics. In this review, we provide information on the alteration of renal transporters by nephrotoxic TCMs, which may be helpful for understanding the nephrotoxic mechanism of TCMs and reducing adverse effects. Studies have proven that when administering nephrotoxic TCMs, the expression or function of renal transporters is altered, especially organic anion transporter 1 and 3. The alteration of these transporters may enhance the accumulation of toxic drugs or the dysfunction of endogenous toxins and subsequently sensitize the kidney to injury.Transporters-related drug combination and clinical biomarkers supervision to avoid the risk of future toxicity are proposed. 展开更多
关键词 Traditional Chinese medicine Nephrotoxicity Renal tubular epithelial cell Organic anion transporter Aristolochic acid Tripterygium wilfordii Hook.f. Rheum officinale Baill
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Broussonin E suppresses LPS-induced inflammatory response in macrophages via inhibiting MAPK pathway and enhancing JAK2-STAT3 pathway 认领 引用 被引量：23
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作者 HUANG Shao-Peng GUAN Xin +6 位作者 KAI Guo-Yin XU Ya-Zhou XU Yuan WANG Hao-Jie PANG Tao ZHANG Lu-Yong LIU Ying 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2019年第5期372-380,共9页
Macrophages play an important role in inflammation, and excessive and chronic activation of macrophages leads to systemic inflammatory diseases, such as atherosclerosis and rheumatoid arthritis. In this paper, we expl... Macrophages play an important role in inflammation, and excessive and chronic activation of macrophages leads to systemic inflammatory diseases, such as atherosclerosis and rheumatoid arthritis. In this paper, we explored the anti-inflammatory effect of broussonin E, a novel phenolic compound isolated from the barks of Broussonetia kanzinoki, and its underlying molecular mechanisms. We discovered that Broussonin E could suppress the LPS-induced pro-inflammatory production in RAW264.7 cells, involving TNF-α, IL-1β, IL-6, COX-2 and iNOS. And broussonin E enhanced the expressions of anti-inflammatory mediators such as IL-10, CD206 and arginase-1(Arg-1) in LPS-stimulated RAW264.7 cells. Further, we demonstrated that broussonin E inhibited the LPS-stimulated phosphorylation of ERK and p38 MAPK. Moreover, we found that broussonin E could activate janus kinase(JAK) 2, signal transducer and activator of transcription(STAT) 3. Downregulated pro-inflammatory cytokines and upregulated anti-inflammatory factors by broussonin E were abolished by using the inhibitor of JAK2-STAT3 pathway, WP1066. Taken together, our results showed that broussonin E could suppress inflammation by modulating macrophages activation state via inhibiting the ERK and p38 MAPK and enhancing JAK2-STAT3 signaling pathway, and can be further developed as a promising drug for the treatment of inflammation-related diseases such as atherosclerosis. 展开更多
关键词 Broussonin E Macrophage polarization Inflammation Janus kinase 2 Signal transducer and activator of transcription 3
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Quantitative proteomics analysis of Fructus Psoraleae-induced hepatotoxicity in rats 认领 引用 被引量：11
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作者 LI Zhi-Jian Abudumijiti Abulizi +10 位作者 XU Deng-Qiu Youlidouzi Maimaiti Silafu Aibai JIANG Zhen-Zhou ZHAO Guo-Lin WANG Tao Aiximujiang Refukati Zulikaer Maimaiti Yiliyaer Simayi CAO Chun-Yu ZHANG Lu-Yong 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2020年第2期123-137,共15页
Fructus Psoraleae,which is commonly consumed for the treatment of osteoporosis,bone fracture,and leucoderma,induces liver injury.This study investigated the pathogenesis of the ethanol extract of Fructus Psoraleae(EEF... Fructus Psoraleae,which is commonly consumed for the treatment of osteoporosis,bone fracture,and leucoderma,induces liver injury.This study investigated the pathogenesis of the ethanol extract of Fructus Psoraleae(EEFP)-induced liver injury in rats.EEFP(1.35,1.80,and 2.25 g·kg^–1)was administrated to Sprague Dawley(SD)rats for 30 d.We measured liver chemistries,histopathology,and quantitative isobaric tags for relative and absolute quantitation(iTRAQ)-based protein profiling.EEFP demonstrated parameters suggestive of liver injury with changes in bile secretion,bile flow rate,and liver histopathology.iTRAQ analysis showed that a total of 4042 proteins were expressed in liver tissues of EEFP-treated and untreated rats.Among these proteins,81 were upregulated and 32 were downregulated in the treatment group.KEGG pathway analysis showed that the drug metabolic pathways of cytochrome P450,glutathione metabolism,glycerolipid metabolism,and bile secretion were enriched with differentially expressed proteins.The expression of key proteins related to the farnesoid X receptor(FXR),i.e.,the peroxisome proliferators-activated receptor alpha(PPAR-α),were downregulated,and multidrug resistance-associated protein 3(MRP3)was upregulated in the EEFP-treated rats.Our results provide evidence that EEFP may induce hepatotoxicity through various pathways.Furthermore,our study demonstrates changes in protein regulation using iTRAQ quantitative proteomics analysis. 展开更多
关键词 Hepatotoxicity iTRAQ Analysis Fructus Psoraleae FXR MRP3
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Protective effect of total flavonoid C-glycosides from Abrus mollis extract on lipopolysaccharide-induced lipotoxicity in mice 认领 引用 被引量：9
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作者 WANG Yun JIANG Zhen-Zhou +7 位作者 CHEN Mi WU Mei-Juan GUO Hong-Li SUN Li-Xin WANG Hao ZHANG Shuang WANG Tao ZHANG Lu-Yong 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2014年第6期461-468,共8页
Abrus mollis is a widely used traditional Chinese medicine for treating acute and chronic hepatitis, steatosis, and fibrosis. It was found that the total flavonoid C-glycosides from Abrus mollis extract(AME) showed po... Abrus mollis is a widely used traditional Chinese medicine for treating acute and chronic hepatitis, steatosis, and fibrosis. It was found that the total flavonoid C-glycosides from Abrus mollis extract(AME) showed potent antioxidant, anti-inflammatory, and hepatoprotective activities. To further investigate the hepatoprotective effect of AME and its possible mechanisms, lipopolysaccharide(LPS)-induced liver injury models were applied in the current study. The results indicated that AME significantly attenuated LPS-induced lipid accumulation in mouse primary hepatocytes as measured by triglyceride(TG) and total cholesterol(TC) assays and Oil Red O staining. Meanwhile, AME exerted a protective effect on LPS-induced liver injury as shown by decreased liver index, serum aminotransferase levels, and hepatic lipid accumulation. Real-time PCR and immunoblot data suggested that AME reversed the LPS-mediated lipid metabolism gene expression, such as sterol regulatory element-binding protein-1(SREBP-1), fatty acid synthase(FAS), and acetyl-CoA carboxylase 1(ACC1). In addition, LPS-induced overexpression of activating transcription factor 4(ATF4), X-box-binding protein-1(XBP-1), and C/EBP homologous protein(CHOP) were dramatically reversed by AME. Furthermore, AME also decreased the expression of LPS-enhanced interleukin-6(IL-6) and cyclooxygenase-2(COX-2). Here, it is demonstrated for the first time that AME ameliorated LPS-induced hepatic lipid accumulation and that this effect of AME can be attributed to its modulation of hepatic de novo fatty acid synthesis. This study also suggested that the hepatoprotective effect of AME may be related to its down-regulation of unfolded protein response(UPR) activation. 展开更多
关键词 Flavonoid C-glycosides Endotoxin Unfolded protein response Lipid metabolism Inflammation
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Anti-inflammatory and hepatoprotective effects of total flavonoid C-glycosides from Abrus mollis extracts 认领 引用 被引量：10
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作者 CHEN Mi WANG Tao +6 位作者 JIANG Zhen-Zhou SHAN Chun WANG Hao WU Mei-Juan ZHANG Shuang ZHANG Yun ZHANG Lu-Yong 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2014年第8期590-598,共9页
The aim of this study was to evaluate the anti-inflammatory and hepatoprotective effects of the total flavonoid C-glycosides isolated from Abrus mollis extracts(AME). In the anti-inflammatory tests, xylene-induced ear... The aim of this study was to evaluate the anti-inflammatory and hepatoprotective effects of the total flavonoid C-glycosides isolated from Abrus mollis extracts(AME). In the anti-inflammatory tests, xylene-induced ear edema model in mice and carrageenan-induced paw edema model in rats were applied. The hepatoprotective effects of AME were evaluated with various in vivo models of acute and chronic liver injury, including carbon tetrachloride(CCl4)-induced hepatitis in mice, D-galactosamine(D-GalN)-induced hepatitis in rats, as well as CCl4-induced hepatic fibrosis in rats. In the acute inflammation experiment, AME significantly suppressed xylene-induced ear edema and carrageenan-induced paw edema, respectively. In the acute hepatitis tests, AME significantly attenuated the excessive release of ALT and AST induced by CCl4 and D-GalN. In CCl4-induced hepatic fibrosis model, AME alleviated liver injury induced by CCl4 shown by histopathological sections of livers and improved liver function as indicated by decreased liver index, serum ALT, AST, TBIL, and ALP levels and hydroxyproline contents in liver tissues, and increased serum ALB and GLU levels. These results indicated that AME possesses potent anti-inflammatory activity in acute inflammation models and hepatoprotective activity in both acute and chronic liver injury models. In conclusion, AME is a potential anti-inflammatory and hepatoprotective agent and a viable candidate for treating inflammation, hepatitis, and hepatic fibrosis. 展开更多
关键词 Abrus mollis Flavonoid C-glycosides Inflammation Liver injury
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Maslinic acid modulates glycogen metabolism by enhancing the insulin signaling pathway and inhibiting glycogen phosphorylase 认领 引用 被引量：9
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作者 LIU Jun WANG Xue +4 位作者 CHEN Yu-Peng MAO Li-Fei SHANG Jing SUN Hong-Bin ZHANG Lu-Yong 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2014年第4期259-265,共7页
AIM: To investigate the molecular signaling mechanism by which the plant-derived, pentacyclic triterpene maslinic acid(MA) exerts anti-diabetic effects. METHOD: HepG2 cells were stimulated with various concentrations ... AIM: To investigate the molecular signaling mechanism by which the plant-derived, pentacyclic triterpene maslinic acid(MA) exerts anti-diabetic effects. METHOD: HepG2 cells were stimulated with various concentrations of MA. The effects of MA on glycogen phosphorylase a(GPa) activity and the cellular glycogen content were measured. Western blot analyses were performed with anti-insulin receptor β(IRβ), protein kinase B(also known as Akt), and glycogen synthase kinase-3β(GSK3β) antibodies. Activation status of the insulin pathway was investigated using phospho-IRβ, as well as phospho-Akt, and phospho-GSK3β antibodies. The specific PI3-kinase inhibitor wortmannin was added to the cells to analyze the Akt expression. Enzyme-linked immunosorbent assay(ELISA) was used to measure the effect of MA on IRβ auto-phosphorylation. Furthermore, the effect of MA on glycogen metabolism was investigated in C57BL/6J mice fed with a high-fat diet(HFD). RESULTS: The results showed that MA exerts anti-diabetic effects by increasing glycogen content and inhibiting glycogen phosphorylase activity in HepG2 cells. Furthermore, MA was shown to induce the phosphorylation level of IRβ-subunit, Akt, and GSK3β. The MA-induced activation of Akt appeared to be specific, since it could be blocked by wortmannin. Finally, MA treatment of mice fed with a high-fat diet reduced the model-associated adiposity and insulin resistance, and increased the accumulated hepatic glycogen content. CONCLUSION: The results suggested that maslinic acid modulates glycogen metabolism by enhancing the insulin signaling pathway and inhibiting glycogen phosphorylase. 展开更多
关键词 Maslinic acid Insulin signal transduction Glycogen phosphorylation a Glycogen metabolism
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DT-13, a saponin of dwarf lilyturf tuber, exhibits anti-cancer activity by down-regulating C-C chemokine receptor type 5 and vascular endothelial growth factor in MDA-MB-435 cells 认领 引用 被引量：7
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作者 ZHAO Ren-Ping LIN Sen-Sen +4 位作者 YUAN Sheng-Tao YU Bo-Yang BAI Xian-Shu SUN Li ZHANG Lu-Yong 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2014年第1期24-29,共6页
AIM： To investigate the anticancer activity of DT-13 under normoxia and determine the underlying mechanisms of action. METHODS： MDA-MB-435 cell proliferation, migration, and adhesion were performed to assess the ant... AIM： To investigate the anticancer activity of DT-13 under normoxia and determine the underlying mechanisms of action. METHODS： MDA-MB-435 cell proliferation, migration, and adhesion were performed to assess the anticancer activity of DT-13, a saponin from Ophiopogonjaponicus, in vitro. In addition, the effects of DT-13 on tumor growth and metastasis in vivo were evaluated by orthotopic implantation of MDA-MB-435 cells into nude mice; mRNA levels of vascular endothelial growth factor （VEGF）, C-C chemokine receptor type 5 （CCR5） and hypoxia-inducible factor 1a （HIF-1a） were evaluated by real-time quantitative PCR; and CCR5 protein levels were detected by Western blot assay. RESULTS： At 0.01 to 1 umol·L -1, DT-13 inhibited MDA-MB-435 cell proliferation, migration, and adhesion significantly in vitro. DT-13 reduced VEGF and CCR5 mRNAs, and decreased CCR5 protein expression by down-regulating HIF-1 a. In addition, DT-13 inhibited MDA-MB-435 cell lung metastasis, and restricted tumor growth slightly in vivo. CONCLUSION： DT-13 inhibited MDA-MB-435 cell proliferation, adhesion, and migration in vitro, and lung metastasis in vivo by reducing VEGF, CCR5, and HIF-la expression. 展开更多
关键词 DT- 13 Saponin Ophiopogonjaponicus Anticancer activity CCR5 VEGF HIF- 1 a
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Marsdenia tenacissima extract suppresses A549 cell migration through regulation of CCR5-CCL5 axis, Rho C, and phosphorylated FAK 认领 引用 被引量：7
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作者 LIN Sen-Sen LI Fang-Fang +5 位作者 SUN Li FAN Wei GU Ming ZHANG Lu-Yong QIN Song YUAN Sheng-Tao 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2016年第3期203-209,共7页
Marsdenia tenacissima, a traditional Chinese medicine, is long been used to treat various diseases including asthma, cancer, trachitis, tonsillitis, pharyngitis, cystitis, and pneumonia. Although Marsdenia tenacissima... Marsdenia tenacissima, a traditional Chinese medicine, is long been used to treat various diseases including asthma, cancer, trachitis, tonsillitis, pharyngitis, cystitis, and pneumonia. Although Marsdenia tenacissima has been demonstrated to have strong anti-tumor effects against primary tumors, its effect on cancer metastasis remains to be defined, and the molecular mechanism underlying the anti-metastatic effect is unknown. In the present study, we investigated the effects of XAP(an extract of Marsdenia tenacissima) on A549 lung cancer cell migration and explored the role of CCR5-CCL5 axis in the anti-metastatic effects of XAP. Our resutls showed that XAP inhibited A549 lung cancer cell migration and invasion in a dose-dependent manner. The protein levels of CCR5, but not CCR9 and CXCR4, were decreased by XAP. The secretion of CCL5, the ligand of CCR5, was reduced by XAP. XAP down-regulated Rho C expression and FAK phosphorylation. In conclusion, XAP inhibited A549 cell migration and invasion through down-regulation of CCR5-CCL5 axis, Rho C, and FAK. 展开更多
关键词 XAP A549 cell migration CCR5-CCL5 axis Rho C FAK
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The role of neutrophils in triptolide-induced liver injury 认领 引用 被引量：5
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作者 WANG Xin-Zhi ZHANG Shen-Ye +2 位作者 XU Yao ZHANG Lu-Yong JIANG Zhen-Zhou 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2018年第9期653-664,共12页
Triptolide(TP) induces severe liver injury, but its hepatotoxicity mechanisms are still unclear. Inflammatory responses may be involved in the pathophysiology. Neutrophils are the first-line immune effectors for steri... Triptolide(TP) induces severe liver injury, but its hepatotoxicity mechanisms are still unclear. Inflammatory responses may be involved in the pathophysiology. Neutrophils are the first-line immune effectors for sterile and non-sterile inflammatory responses. Thus, the aim of the present study was to investigate the neutrophilic inflammatory response in TP-induced liver injury in C57 BL/6 mice. Our results showed that neutrophils were recruited and accumulated in the liver, which was parallel to or slightly after the development of liver injury. Neutrophils induced release of myeloperoxidase and up-regulation of CD11 b, which caused cytotoxicity and hepatocyte death. Hepatic expressions of CXL1, TNF-α, IL-6, and MCP1 were increased significantly to regulate neutrophils recruitment and activation. Up-regulation of toll like receptors 4 and 9 also facilitated neutrophils infiltration. Moreover, neutrophils depletion using an anti-Gr1 antibody showed mild protection against TP overdose. These results indicated that neutrophils accumulation might be the secondary response, not the cause of TP-induced liver injury. In conclusion, the inflammatory response including neutrophil infiltration may play a role in TP-induced hepatotoxicity, but may not be severe enough to cause additional liver injury. 展开更多
关键词 Triptolide Liver injury Inflammatory response Neutrophil Depletion
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Asiatic acid mitigates hyperglycemia and reduces islet fibrosis in Goto-Kakizaki rat,a spontaneous type 2 diabetic animal model 认领 引用 被引量：5
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作者 WANG Xue LU Qian +5 位作者 YU Dong-Sheng CHEN Yu-Peng SHANG Jing ZHANG Lu-Yong SUN Hong-Bin LIU Jun 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2015年第7期529-534,共6页
The Goto-Kakizaki(GK) rat is a spontaneous type 2 diabetic animal model,which is characterized by a progressive loss of beta islet cells with fibrosis.In the present study,the hypoglycemic effect of asiatic acid(AA) i... The Goto-Kakizaki(GK) rat is a spontaneous type 2 diabetic animal model,which is characterized by a progressive loss of beta islet cells with fibrosis.In the present study,the hypoglycemic effect of asiatic acid(AA) in GK rats was examined.GK rats receiving AA at a daily dose of 25 mg·kg-1 for four weeks showed a significant reduction in blood glucose levels.Age-matched normal Wistar rats were given 0.5% sodium carboxymethyl cellulose(CMC-Na) solution for the same periods and used as control.Compared to the normal Wistar rats,GK rats treated with AA showed improvement in insulin resistance partially through decreasing glucose level(P < 0.01) and insulin level(P < 0.05).Furthermore,the results of immunohistochemistry indicate that AA treatment reduced islet fibrosis in GK rats.Fibronectin,a key protein related to islet fibrosis,was over-expressed in GK rats,which was reversed significantly by AA treatment(P < 0.05).These findings suggest that AA has a beneficial effect on lowering blood glucose levels in GK rats and improves fibrosis of islets in diabetes,which may play a role in the prevention of islets 展开更多
关键词 Asiatic acid Hyperglycemia Islet fibrosis Goto-Kakizaki(GK) rats Diabetes mellitus Pancreas
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Altered integrity of hepatocyte tight junctions in rats with triptolide-induced cholestasis 认领 引用 被引量：3
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作者 HUANG Shan LIU Li +9 位作者 MEI Hui-Fang ZHANG Qian-Wen ZHANG Xi XU Xiao-Ting WANG Xin-Zhi HUANG Xin WANG Tao JIANG Zhen-Zhou ZHANG Lu-Yong SUN Li-Xin 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2021年第3期188-194,共7页
Triptolide(TP),an active component of Tripterygium wilfordii Hook.f.(TWHF),has been widely used for centuries as a traditional Chinese medicine.However,the clinical application of TP has been restricted due to multita... Triptolide(TP),an active component of Tripterygium wilfordii Hook.f.(TWHF),has been widely used for centuries as a traditional Chinese medicine.However,the clinical application of TP has been restricted due to multitarget toxicity,such as hepatotoxicity.In this study,28 days of oral TP administration(100,200,or 400μg·kg-1·d-1)induced the occurrence of cholestasis in female Wistar rats,as evidenced by increased serum levels ofγ-glutamyl transpeptidase(γ-GGT),alkaline phosphatase(ALP)and hepatic total bile acids(TBAs).In addition,the heptocyte polarity associated with the strcture of tight junctions(TJs)was disrupted in both rats and sandwich-cultured primary hepatocytes.Immunoblotting revealed decreased expression of the TJ-associated proteins occludin,claudin-1,and zonula occludens protein(ZO-1),and downregulated m RNA levels of these TJs was also detected by real-time PCR.An immunofluorescence analysis showed abnormal subcellular localization of occludin,claudin-1 and ZO-1,which was also confirmed by transmission electron microscopy.Moreover,the concentration of FITC-dextran,a marker of paracellular penetration,was found to increase rapidly in bile increased rapidly(within 6 minutes)after treatment with TP,which indicated the functional impairment of TJs.Taken together,these results suggest that the administration of TP for 28 consecutive days to rats could induce cholestatic injury in the liver,and the increased paracellular permeability might play an important role in these pathological changes. 展开更多
关键词 Triptolide Cholestatic injury Tight junction Sandwich-cultured primary hepatocytes
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The saponin DT-13 inhibits gastric cancer cell migration through down-regulation of CCR5-CCL5 axis 认领 引用 被引量：7
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作者 LIN Sen-Sen FAN Wei +5 位作者 SUN Li LI Fang-Fang ZHAO Ren-Ping ZHANG Lu-Yong YU Bo-Yang YUAN Sheng-Tao 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2014年第11期833-840,共8页
AIM: To investigate the effect of DT-13 on gastric cancer cell migration, and to explore the possible mechanisms underlying the anti-metastasis activity of DT-13. METHODS: Growth inhibition of DT-13 was analyzed by th... AIM: To investigate the effect of DT-13 on gastric cancer cell migration, and to explore the possible mechanisms underlying the anti-metastasis activity of DT-13. METHODS: Growth inhibition of DT-13 was analyzed by the MTT assay. Cell migration was measured by the scratch-wound assay and transwell double chamber assay. To investigate the possible mechanisms underlying the anti-metastasis activity of DT-13, chemokine receptors that are involved in cancer metastasis(CCR2, CCR5, CCR7, CXCR4, and CXCR6) were detected by conventional PCR. The effect of DT-13 on CCR5 and CXCR4 expression was further evaluated by quantitative PCR and Western blot, respectively. The secretion of CCL5(ligand of CCR5) and SDF-1(ligand of CXCR4) were detected by enzyme-linked immunosorbent assay(ELISA). RESULTS: DT-13 inhibited BGC-823 and HGC-27 cell growth in a dose dependent manner, and the estimated IC50 value for 24 h treatment was 23.5 ± 5.1 μmol·L-1 for BGC-823 cells and 35.6 ± 7.6 μmol·L-1 for HGC-27 cells. DT-13 also significantly decreased gastric cancer cell migration. DT-13 significantly decreased the gene expression of CCR5 in both BGC-823 and HGC-27 gastric cancer cells, and moderately reduced the expression of CXCR4. Similar to the results of gene expression, significant down-regulation of CCR5 protein was observed, but CXCR4 protein levels were much less affected. CCL5 secretion, but not SDF-1 production, was inhibited by DT-13. CONCLUSION: DT-13 inhibited gastric cancer cell migration by down-regulation of the CCR5-CCL5 axis. 展开更多
关键词 Liriope muscari Saponin DT-13 BGC-823 HGC-27 Gastric cancer cell migration CCR5 CCL5 CXCR4
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Screening of natural compounds with neuronal differentiation promoting effects in a cell-based model 认领 引用 被引量：1
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作者 CHEN Tao WANG Juan +2 位作者 LIU Mei ZHANG Lu-Yong LIAO Hong 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2015年第8期602-608,共7页
The purpose of this study was to establish a drug screening method for small molecules extracted from traditional Chinese medicines(TCM) that have neuronal differentiation promoting effects, using P19 embryonic carcin... The purpose of this study was to establish a drug screening method for small molecules extracted from traditional Chinese medicines(TCM) that have neuronal differentiation promoting effects, using P19 embryonic carcinoma cell as a cell-based model. First, the constructed plasmid(p Tα1-Luc) was transfected into P19 cells to establish a screening model. Second, several TCMs were screened using the established model and all-trans-retinoic acid as a positive control. Finally, the underlying molecular mechanism was explored using immunofluorescence staining, q T-PCR, and Western blot analysis. Our results indicated that the drug screen model was established successfully and that both honokiol and hyperoside induced P19 differentiation into neurons, with the possible molecular mechanism being modulating the Wnt signaling pathway. In conclusion, the drug screening model developed in the present study provides a rapid, cell-based screening platform for identifying natural compounds with neuronal differentiation effects. 展开更多
关键词 Drug screening model Neuronal differentiation P19 embryonic carcinoma cells Wnt signaling pathway Traditional Chinese Medicine
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Antagonistic effects of extracts from Artemisia rupetris L. and Leontopodium leontopodioides to CC chemokine receptor 2b(CCR2b) 认领 引用
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作者 YU Qin-Wei HU Jie +7 位作者 WANG Hao CHEN Xin ZHAO Fang GAO Peng YANG Qiu-Bin SUN Dan-Dan ZHANG Lu-Yong YAN Ming 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2016年第5期363-369,共7页
The present study was designed to establish a suitable assay to explore CCR2 b receptor antagonists from the natural products of Artemisia rupetris and Leontopodium leontopodioides. An aequorin assay was developed as ... The present study was designed to establish a suitable assay to explore CCR2 b receptor antagonists from the natural products of Artemisia rupetris and Leontopodium leontopodioides. An aequorin assay was developed as a cell-based assay suitable for 384-well microplate and used for screening CCR2 b receptor antagonists from natural products. Through establishing suitable conditions, the assay was shown to be suitable for screening of CCR2 b receptor antagonists. Seven compounds were identified in preliminary screening. Five of them showed evident dose-response relationship in secondary screening. The structure–activity relationship study suggested that 7-position hydroxyl group of flavonoids was necessary, a polar group should be introduced on the 3-position, and the substituents on 2-position benzene ring of flavonoids have little influence on the potentency of the inhibition activity on CCR2 b receptor. The ortho-position dihydroxyl structure in quinic acid compounds may be important. In conclusion, Compounds HR-1, 5, 7, and AR-20, 35 showed activity as antagonist of CCR2 b receptor, which shed lights on the development of novel drugs as CCR2 b receptor antagonists for preventing inflammation related diseases. 展开更多
关键词 CCR2b antagonist Inflammation Aequorin assay Artemisia rupetris Leontopodium leontopodioides
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Tripterygium wilfordii multiglycoside-induced hepatotoxicity via inflammation and apoptosis in zebrafish 认领 引用 被引量：7
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作者 DUAN Xiu-Ying MA Rui-Jiao +4 位作者 HSIAO Chung-Der JIANG Zhen-Zhou ZHANG Lu-Yong ZHANG Yun LIU Ke-Chun 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2021年第10期750-757,共8页
Tripterygium wilfordii multiglycoside(GTW)is a commonly used compound for the treatment of rheumatoid arthritis(RA)and immune diseases in clinical practice.However,it can induce liver injury and the mechanism of hepat... Tripterygium wilfordii multiglycoside(GTW)is a commonly used compound for the treatment of rheumatoid arthritis(RA)and immune diseases in clinical practice.However,it can induce liver injury and the mechanism of hepatotoxicity is still not clear.This study was designed to investigate GTW-induced hepatotoxicity in zebrafish larvae and explore the mechanism involved.The 72 hpf(hours post fertilization)zebrafish larvae were administered with different concentrations of GTW for three days and their mortality,malformation rate,morphological changes in the liver,transaminase levels,and histopathological changes in the liver of zebrafish larvae were detected.The reverse transcription-polymerase chain reaction(RT-PCR)was used to examine the levels of microRNA-122(miR-122)and genes related to inflammation,apoptosis,cell proliferation and liver function.The results showed that GTW increased the mortality of zebrafish larvae,while significant malformations and liver damage occurred.The main manifestations were elevated levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST),significant liver atrophy,vacuoles in liver tissue,sparse cytoplasm,and unclear hepatocyte contours.RT-PCR results showed that the expression of miR-122 significantly decreased by GTW;the mRNA levels of inflammation-related genes il1β,il6,tnfα,il10,cox2 and ptges significantly increased;the mRNA level of tgfβsignificantly decreased;the mRNA levels of apoptosis-related genes,caspase-8 and caspase-9,significantly increased;the mRNA level of bcl2 significantly decreased;the mRNA levels of cell proliferation-related genes,top2αand uhrf1,significantly reduced;the mRNA levels of liver function-related genes,alr and cyp3c1,significantly increased;and the mRNA level of cyp3a65 significantly decreased.In zebrafish,GTW can cause increased inflammation,enhanced apoptosis,decreased cell proliferation,and abnormal expression of liver function-related genes,leading to abnormal liver structure and function and resulting in hepatotoxicity. 展开更多
关键词 Tripterygium wilfordii multiglycoside Zebrafish Hepatotoxicity Inflammation Apoptosis
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Triptolide reduces prostate size and androgen level on testosterone-induced benign prostatic hyperplasia in Sprague Dawley rats 认领 引用 被引量：9
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作者 WANG Yu-Rong XU Yuan +2 位作者 JIANG Zhen-Zhou ZHANG Lu-Yong WANG Tao 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2017年第5期341-346,共6页
Benign prostatic hyperplasia(BPH) is an age-related disease of unknown etiology, characterized by prostatic enlargement coincident with distinct alterations in tissue histology. In the present study, we investigated w... Benign prostatic hyperplasia(BPH) is an age-related disease of unknown etiology, characterized by prostatic enlargement coincident with distinct alterations in tissue histology. In the present study, we investigated whether triptolide can prevent testosterone-induced prostatic hyperplasia in rats. Castration was performed via the scrotal route after urethane aesthesia. BPH was induced in experimental groups by daily subcutaneous injections of testosterone propionate(TP) for two weeks. Triptolide was administered daily by oral gavage at a dose of 100 and 50 μg×kg-1 for 2 weeks, along with the TP injections. On day 14, the animals were humanely killed by cervical dislocation after aesthesia. Prostates were excised, weighed, and used for histological studies. Testosterone and dihydrotestosterone(DHT) levels in serum and prostate were measured. The results showed that triptolide significantly reduced the prostate weight, and the testosterone and DHT levels in both the serum and prostate. Histopathological examination also showed that triptolide treatment suppressed TP-induced prostatic hyperplasia. In conclusion, triptolide effectively inhibits the development of BPH induced by testosterone in a rat model. 展开更多
关键词 Triptolide Benign prostatic hyperplasia Androgen
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Inhibitory effects of Tripterygium wilfordii multiglycoside on benign prostatic hyperplasia in rats 认领 引用 被引量：5
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作者 SHEN Hai-Nan XU Yuan +3 位作者 JIANG Zhen-Zhou HUANG Xin ZHANG Lu-Yong WANG Tao 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2015年第6期421-427,共7页
The present study was designed to evaluate the inhibitory effects of Tripterygium wilfordii multiglycoside(GTW) against testosterone-induced benign prostatic hyperplasia(BPH) in rats. A total of 45 rats were randomly ... The present study was designed to evaluate the inhibitory effects of Tripterygium wilfordii multiglycoside(GTW) against testosterone-induced benign prostatic hyperplasia(BPH) in rats. A total of 45 rats were randomly divided into five groups: Group I, vehicle control group(sham-operated and treated with vehicle); Group II, BPH group; Group III, BPH rats treated with finasteride at a dose of 5 mg·kg-1; and Groups IV and V, BPH rats treated with GTW at dose levels of 10 and 20 mg·kg-1, respectively. The drugs were administered orally once a day for 14 days. Prostate weight, prostatic index, and the testosterone and dihydrotestosterone(DHT) levels in serum and prostate, and the serum prostate specific antigen(PSA) levels were measured; prostate tissues were taken for histopathological examination; and serum biochemical analysis was also performed. The BPH rats displayed an increase in prostate weight, prostatic index with increased testosterone and DHT levels in both the serum and prostate, and increased serum PSA levels. GTW treatment at both doses resulted in significant reductions in prostate weight, prostatic index, testosterone and DHT levels in both the serum and prostate, and serum PSA levels, compared with BPH group. Histopathological examination also indicated that GTW treatment at both doses inhibited testosterone-induced prostatic hyperplasia. Serum biochemical analysis showed that the liver and renal functions were normal. In conclusion, GTW inhibited testosterone-induced prostatic hyperplasia in rats, without host toxicity, providing a basis for the development of GTW as a novel therapy for BPH. 展开更多
关键词 Tripterygium wilfordii Multiglycoside Benign prostatic hyperplasia Testosterone Dihydrotestosterone
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Quercetin promotes neurite growth through enhancing intracellular cAMP level and GAP-43 expression 认领 引用 被引量：7
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作者 CHEN Ming-Ming YIN Zhi-Qi +1 位作者 ZHANG Lu-Yong LIAO Hong 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2015年第9期667-672,共6页
The present study was designed to investigate the role of quercetin on neurite growth in N1E-115 cells and the underlying mechanisms. Quercetin was evaluated for its effects on cell numbers of neurites, neurite length... The present study was designed to investigate the role of quercetin on neurite growth in N1E-115 cells and the underlying mechanisms. Quercetin was evaluated for its effects on cell numbers of neurites, neurite length, intracellular cAMP content, and Gap-43 expression in N1E-115 cells in vitro by use of microscopy, LANCE? cAMP 384 kit, and Western blot analysis, respectively. Our results showed that quercetin could increase the neurite length in a concentration-dependent manner, but had no effect on the numbers of cells. Quercetin significantly increased the expression of cellular cAMP in a time- and concentration-dependent manner. The Gap-43 expression was up-regulated in a time-dependent manner. In conclusion, quercetin could promote neurite growth through increasing the intracellular c AMP level and Gap-43 expression. 展开更多
关键词 Quercetin cAMP Gap-43 neurite growth N1E-115
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