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Identifying reliable reference genes for qPCR in skinks under different thermal stress 认领 引用
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作者 Ziwen Yang Lining Chen +4 位作者 Wenning Zhang Huiyuan Wu Jiehong Ji Jiayong Zhang Danna Yu 《Zoological Systematics》 CSCD 2026年第2期150-162,共13页
Global warming has emerged as the predominant trend in global climate change,with forecasts suggesting a rise in the occurrence and severity of extreme weather phenomena.As a result,ectothermic animals have become ide... Global warming has emerged as the predominant trend in global climate change,with forecasts suggesting a rise in the occurrence and severity of extreme weather phenomena.As a result,ectothermic animals have become ideal model organisms for investigating the potential impacts of climate change on global biodiversity.Real-time fluorescence quantitative PCR(RT-qPCR)is a widely used technique for gene expression analysis.However,stable reference genes suitable for accurate normalization of transcriptional data across lizard samples have not yet been established.In this study,three skink species-Scincella modesta,S.reevesii,and Ateuchosaurus chinensis-were selected as model organisms to evaluate the expression stability of eight candidate housekeeping genes(18S rRNA,EF1α,ACTB,GAPDH,HPRT1,YWHAZ,RPS15,and RPS18)under nine distinct temperature conditions(6℃,10℃,14℃,18℃,22℃,25℃,28℃,32℃,and 36℃).The Delta Ct method,BestKeeper,NormFinder,GeNorm,and RefFinder were utilized to assess expression consistency and to identify the most suitable reference genes,as well as the ideal number needed for reliable normalization.The results indicated that under varying temperature conditions,the best-performing internal controls were RPS18 and RPS15 for S.modesta;RPS18 and EF1α for S.reevesii;and RPS15 and YWHAZ for A.chinensis.Furthermore,RPS18,RPS15,and EF1α were identified as a stable and commonly applicable set of internal controls for RT-qPCR normalization across all three skink species and are recommended as reliable reference genes for use in related lizards exposed to thermal stress. 展开更多
关键词 Skink normalization reference genes RefFinder
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Insights into gene rearrangement of Cnipsomorpha erinacea and Paragongylopus plaumanni and their phylogenetic relationships based on seventy-eight mitochondrial genomes from stick insects(Phasmatodea) 认领 引用
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作者 Wenhui Yang TongLin +4 位作者 Huiyuan Wu Ziwen Yang Jiehong Ji Jiayong Zhang Danna Yu 《Zoological Systematics》 CSCD 2026年第3期178-194,共17页
To resolve stick insect phylogeny and mitochondrial rearrangement,mitochondrial genomes were sequenced.A total of ten mitochondrial genomes,15,808–16,608 bp in length,were successfully sequenced in this study.Gene re... To resolve stick insect phylogeny and mitochondrial rearrangement,mitochondrial genomes were sequenced.A total of ten mitochondrial genomes,15,808–16,608 bp in length,were successfully sequenced in this study.Gene rearrangements were observed in three samples of two species:Paragongylopus plaumanni(sample number:WSZJC6 and YNZJC5),and Cnipsomorpha erinacea.The tandem duplication–random loss(TDRL)model provides a reasonable explanation for the detected gene rearrangements.Specifically,C.erinacea exhibits a gene rearrangement pattern of 12SrRNA-CR1-trn I-CR2-trn Q-trnM.P.plaumanni(WSZJC6)displays a CR-trnM-NCR-trn I-trnQ-ND2 gene arrangement,whereas P.plaumanni(YNZJC5)displays a more complex gene arrangement:a CR-trn M-NCR1-trnI-trnQ-NCR2-ND2.In addition to identifying novel gene structures,we analyzed codon usage and base composition across the 10 mitochondrial genomes.Bayesian inference(BI)and Maximum likelihood(ML)methods were employed to infer phylogenetic trees,utilizing the PCGs_123 and PCGs_12datasets.When the unstable taxon Stheneboea repudiosa was excluded from the analysis,the subfamilies Lonchodinae and Necrosciinae were recovered as monophyletic.However,these two groups were not supported as sister lineages.Moreover,species Achrioptera manga was found to cluster with members of the family Bacillidae,resulting in the non-monophyly of both Phasmatidae and Bacillidae.The phylogenetic analysis of the three subfamilies within Pseudophasmatidae recovered the topology((Pseudophasmatinae)+(Obriminae+Dataminae)),consistent with previous studies.Additionally,Heteropterygidae and Pseudophasmatidae formed a sister clade,while Necrosciinae and Phasmatidae formed another.Bacillidae was positioned between these two major clades,resulting in the overall topology:(Heteropterygidae+Pseudophasmatidae)+((Bacillidae)+(Necrosciinae+Phasmatidae)). 展开更多
关键词 Phasmatodea mitogenomes phylogenetic analysis rearrangement RSCU
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Distributed optimal formation control of heterogeneous Euler–Lagrange multi-agent systems 认领 引用
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作者 Mengmeng Duan Fengping Huang +2 位作者 Shanying Zhu Ziwen Yang Cailian Chen 《Journal of Automation and Intelligence》 CSCD 2025年第4期282-290,共9页
In this paper,the distributed optimal formation control problem of heterogeneous Euler–Lagrange multi-agent systems with generic formation constraints and inequality constraints is investigated.Based on the primal–d... In this paper,the distributed optimal formation control problem of heterogeneous Euler–Lagrange multi-agent systems with generic formation constraints and inequality constraints is investigated.Based on the primal–dual dynamics and the adaptive control technique,a distributed optimal formation controller consists of a velocity reference signal generator and a velocity tracking controller is proposed.By using the optimality condition,the relationship between the equilibrium point of the closed-loop system and the optimal solution of the optimization problem is established.Then,by utilizing Lyapunov stability analysis,it is rigorously proved that the optimal formation is reached with the proposed controller.Lastly,simulation examples are provided to substantiate the theoretical results. 展开更多
关键词 Formation control Distributed optimization Multi-agent systems
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Multiomics and blood-based biomarkers of moyamoya disease:protocol of Moyamoya Omics Atlas(MOYAOMICS) 认领 引用 被引量：1
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作者 Peicong Ge Zihan Yin +43 位作者 Chuming Tao Chaofan Zeng Xiaofan Yu Shixiong Lei Junsheng Li Yuanren Zhai Long Ma Qiheng He Chenglong Liu Wei Liu Bojian Zhang Zhiyao Zheng Siqi Mou Zhikang Zhao Shuang Wang Wei Sun Min Guo Shuai Zheng Jia Zhang Xiaofeng Deng Xingju Liu Xun Ye Qian Zhang Rong Wang Yan Zhang Shaosen Zhang Chengjun Wang Ziwen Yang Nijia Zhang Mingxing Wu Jian Sun Yujia Zhou Zhiyong Shi Yonggang Ma Jianpo Zhou Shaochen Yu Jiaxi Li Junli Lu Faliang Gao Wenjing Wang Yanming Chen Xingen Zhu Dong Zhang Jizong Zhao 《Chinese Neurosurgical Journal》 CAS CSCD 2024年第3期211-219,共9页
Background Moyamoya disease(MMD)is a rare and complex cerebrovascular disorder characterized by the progressive narrowing of the internal carotid arteries and the formation of compensatory collateral vessels.The etiol... Background Moyamoya disease(MMD)is a rare and complex cerebrovascular disorder characterized by the progressive narrowing of the internal carotid arteries and the formation of compensatory collateral vessels.The etiology of MMD remains enigmatic,making diagnosis and management challenging.The MOYAOMICS project was initiated to investigate the molecular underpinnings of MMD and explore potential diagnostic and therapeutic strategies.Methods The MOYAOMICS project employs a multidisciplinary approach,integrating various omics technologies,including genomics,transcriptomics,proteomics,and metabolomics,to comprehensively examine the molecular signatures associated with MMD pathogenesis.Additionally,we will investigate the potential influence of gut microbiota and brain-gut peptides on MMD development,assessing their suitability as targets for therapeutic strategies and dietary interventions.Radiomics,a specialized field in medical imaging,is utilized to analyze neuroimaging data for early detection and characterization of MMD-related brain changes.Deep learning algorithms are employed to differentiate MMD from other conditions,automating the diagnostic process.We also employ single-cellomics and mass cytometry to precisely study cellular heterogeneity in peripheral blood samples from MMD patients.Conclusions The MOYAOMICS project represents a significant step toward comprehending MMD’s molecular underpinnings.This multidisciplinary approach has the potential to revolutionize early diagnosis,patient stratification,and the development of targeted therapies for MMD.The identification of blood-based biomarkers and the integration of multiple omics data are critical for improving the clinical management of MMD and enhancing patient outcomes for this complex disease. 展开更多
关键词 multiomics blood based biomarkers moyaomics project compensatory collateral vesselsthe cerebrovascular disorder molecular underpinnings narrowing internal carotid arteries
Community structure and carbon metabolism functions of bacterioplankton in the Guangdong coastal zone 认领 引用 被引量：1
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作者 Ziqi Peng Pandeng Wang +9 位作者 Xiaoqing Luo Qiqi Deng Ziwen Yang Jiaxue Wu Wendong Xian Weicong Yan Xiaozhen Mou Yang Yuan Wenjun Li Jialing Li 《Marine Life Science & Technology》 SCIE CSCD 2024年第3期547-561,共15页
Coastal ecosystems are an important region for biogeochemical cycling,are a hotspot of anthropogenic disturbance and play a crucial role in global carbon cycling through the metabolic activities of bacterioplankton.Ba... Coastal ecosystems are an important region for biogeochemical cycling,are a hotspot of anthropogenic disturbance and play a crucial role in global carbon cycling through the metabolic activities of bacterioplankton.Bacterioplankton can be broadly classified into two lifestyles:free-living(FL)and particle-attached(PA).However,how coastal bacterioplankton the community structure,co-occurrence networks and carbon metabolic functions with different lifestyles are differentiated is still largely unknown.Understanding these processes is necessary to better determine the contributions of coastal bacterioplankton to carbon cycling.Here,the characteristics of community structure and carbon metabolism function of bacterioplankton with two lifestyles in the coastal areas of Guangdong Province were investigated using amplicon sequencing,metagenomic,and metatranscriptomic techniques.The results show that the main bacterioplankton responsible for carbon metabolism were the Pseudomonadota,Bacteroidota,and Actinomycetota.The microbial community structure,carbon metabolic function,and environmental preferences differ between different lifestyles.FL and PA bacteria exhibited higher carbon fixation and degradation potentials,respectively.A range of environmental factors,such as dissolved oxygen,pH,and temperature,were associated with the community structure and carbon metabolic functions of the bacterioplankton.Human activities,such as nutrient discharge,may affect the distribution of functional genes and enhance the carbon degradation functions of bacterioplankton.In conclusion,this study increased the understanding of the role of microorganisms in regulating carbon export in coastal ecosystems with intense human activity. 展开更多
关键词 Particle-attached and free-living bacterioplankton Community structure Carbon metabolism Coastal ecosystem
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Dual-functional Cu2O/g-C3N4 heterojunctions: a high-performance SERS sensor and photocatalytic self-cleaning system for water pollution detection and remediation 认领 引用 被引量：1
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作者 Shuo Yang Kaiyue Li +5 位作者 Ping Huang Keyan Liu Wenhui Li Yuquan Zhuo Ziwen Yang Donglai Han 《Microsystems & Nanoengineering》 SCIE EI CSCD 2024年第6期325-340,共16页
This study introduces a multifunctional device based on Cu2O/g-C3N4 monitoring and purification p–n heterojunctions(MPHs),seamlessly integrating surface-enhanced Raman scattering(SERS)detection with photocat... This study introduces a multifunctional device based on Cu2O/g-C3N4 monitoring and purification p–n heterojunctions(MPHs),seamlessly integrating surface-enhanced Raman scattering(SERS)detection with photocatalytic degradation capabilities.The SERS and photocatalytic performances of the Cu2O in various morphologies,g-C3N4 nanosheets(NSs)and Cu2O/g-C3N4 MPHs with different g-C3N4 mass ratios were systematically evaluated,with a particular emphasis on the Cu2O/g-C3N4-0.2 MPH,where g-C3N4 constituted 20%of the total mass.Multiple optical and electrochemical tests revealed that the Cu2O/g-C3N4-0.2 MPH effectively enhances charge separation and reduces charge transfer resistance.The Cu2O/g-C3N4-0.2 SERS sensor exhibited a relative standard deviation(RSD)below 15%and achieved an enhancement factor(EF)of 2.43×106 for 4-ATP detection,demonstrating its high sensitivity and consistency.Additionally,it demonstrated a 98.3%degradation efficiency for methyl orange(MO)under visible light within 90 min.Remarkably,even after 216 days,its photocatalytic efficiency remained at 93.7%,and it retained an 84.0%efficiency after four cycles.XRD and SEM analyses before and after cycling,as well as after 216 days,confirmed the structural and morphological stability of the composite,demonstrating its cyclic and long-term stability.The excellent performance of the Cu2O/g-C3N4 MPH is attributed to its Z-type mechanism,as verified by radical trapping experiments.The evaluation of the self-cleaning performance of the Cu2O/g-C3N4-0.2 SERS sensor demonstrated that its Z-scheme structure not only provides excellent self-cleaning capability but also enables the detection of both individual and mixed pollutants,while significantly enhancing the SERS signal response through an effective charge transfer enhancement mechanism. 展开更多
关键词 mechanism stability performance
Leader-Following Entrapping Control of AUVs Using Bearing Measurements in Local Coordinate Frames 认领 引用 被引量：2
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作者 Yingbo Fu Ziwen Yang +1 位作者 Shanying Zhu Cailian Chen 《Guidance, Navigation and Control》 2022年第4期85-121,共37页
In this paper,the entrapping control problem of discrete-time AUVs with local coordinate frames is studied.To achieve entrapment in arbitrarily shaped orbits and formations,we design a bearingbased leader-following fr... In this paper,the entrapping control problem of discrete-time AUVs with local coordinate frames is studied.To achieve entrapment in arbitrarily shaped orbits and formations,we design a bearingbased leader-following framework fully in the discrete-time domain with four parts:the orientation estimation unit,estimator unit,controller unit and parameters tuning unit.With bearing measurements and communication information,the orientation estimation unit can estimate orientations of local coordinate frames infinite time,and the estimator unit can achieve local localization.Based on estimation,the controller unit can drive each AUV to track the desired orbit or formation with an arbitrary shape.We present su±cient conditions under which stability of the overall system is proved using the theorem offinite-time stability and LaSalle's theorem for the discrete-time system.Moreover,the parameters tuning unit can calculate optimal parameters to improve overall performance.Additionally,we extend our schemes to nonholonomic AUVs with unicycle models.Finally,simulation results demonstrate the effectiveness of the proposed scheme. 展开更多
关键词 Entrapping control discrete-time AUVs bearing measurements local coordinate frames
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