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Biosensing analysis based on Au-Cu bimetallic nanoclusters for colorimetric and fluorescent detection of aflatoxin B1in cereals and milk 认领 引用
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作者 Hui Deng Songlin Liu +6 位作者 Xin Deng Xu Wang Ying Xiong Qian Wen Wang Li Yanni Chen Jiali Ren 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第2期869-878,共10页
Aflatoxin B1(AFB1)in food is a threaten to food safety and human health.In this study,a colorimetric and fluorescent dual-mode aptamer sensor was constructed for rapid and accurate detection of AFB1based on p... Aflatoxin B1(AFB1)in food is a threaten to food safety and human health.In this study,a colorimetric and fluorescent dual-mode aptamer sensor was constructed for rapid and accurate detection of AFB1based on polyvinylpyrrolidone-gold-copper bimetallic nanoclusters(PVP-Au/Cu NCs)and Mn3O4nanoparticles(Mn3O4NPs).In the presence of AFB1,the AFB1aptamer on the surface of Mn3O4NPs specifically bound to AFB1.The oxidase-like activity of Mn3O4NPs was restored and 3,3',5,5'-0tetramethylbenzidine(TMB)was oxidized to oxidized TMB(ox TMB),which quenched the orange fluorescence of the PVP-Au/Cu NCs.With AFB1concentrations increasing,the blue color of ox TMB deepened,the fluorescence quenching efficiency was enhanced.The working range was 0–56μg/Lwith a limit of detection(LOD)of 0.36μg/L(colorimetric),and2.86–40.00μg/L with a LOD of 0.29μg/L(fluorescent).In addition,the established sensor was successfully applied to determine AFB1in milk,rice,oats,and corn,with a recovery rate of 91.1%–107.5%.This study provides an insight for fabricating a sensitive,efficient,and accurate analytical platform for rapid detection of AFB1in food. 展开更多
关键词 Aflatoxin B1 Dual-mode Bimetallic nanoclusters Oxidase-like activity
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Curcumin mitigated aflatoxin B1-induced endoplasmic reticulum stress and gut-kidney axis damage in sheep by regulating the ATF6/GRP78 and IL-1β/NF-κB signaling pathways 认领 引用
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作者 Ting Wang Chuanqi Wang +3 位作者 Tongwei Liu Jiefeng Li Hengtong Fang Jing Zhang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第3期1649-1673,共25页
Background Aflatoxin B1(AFB1),a toxic secondary metabolite produced by Aspergillus flavus and Aspergillus parasiticus,is widely present in various crops and induces endoplasmic reticulum stress in the intestine ... Background Aflatoxin B1(AFB1),a toxic secondary metabolite produced by Aspergillus flavus and Aspergillus parasiticus,is widely present in various crops and induces endoplasmic reticulum stress in the intestine and kidney of animals,leading to apoptosis and inflammatory damage.Curcumin is a natural phenolic antioxidant that has antioxidant,anti-apoptotic and anti-inflammatory effects.However,the role and mechanism of curcumin in alleviating the toxicity of AFB1 in sheep remain unclear.Therefore,this study aimed to investigate the mitigating effects of curcumin on intestinal microbiota disorders and intestinal and kidney injuries in AFB1-exposed sheep.Eighteen sheep were randomly divided into three treatment groups.The groups were the control group(CON,basal diet),the AFB1 group(AFB1,basic diet+500μg/kg DM AFB1),and the AFB1_Curcumin group(AFB1_CUR,basic diet+500μg/kg DM AFB1+800 mg/kg DM curcumin)for 21 d.Results AFB1 induced intestinal barrier dysfunction,intestinal flora imbalance,and intestinal mucosal damage.Curcumin addition inhibited the activity of the ATF6/GRP78 and IL-1β/NF-κB signaling pathways to alleviate kidney injury and activated the NRF2/KEAP1 pathway and antioxidant system to reduce the toxic substances cycle in the intestine-kidney axis(P<0.05).The protective effects of curcumin on the intestine and kidney are related to a reduction in the levels of Prevotella ruminicola and Ruminococcus albus.Therefore,the structure of the microbiota and antioxidant functions were improved,mitigating damage to the intestine-kidney axis.Conclusions Curcumin can alleviate AFB1-induced disorder of the intestinal microbiota by enhancing intestinal barrier function;reducing intestinal apoptosis,oxidative stress,and inflammatory damage;and regulating the intestinal microbiota via the intestine-kidney axis.Moreover,the activity of the ATF6/GRP78 and IL-1β/NF-κB signaling pathways was inhibited by curcumin to mitigate intestine-kidney axis injury.Additionally,activating the NRF2/KEAP1 signaling pathway promotes the function of biological antioxidant system. 展开更多
关键词 Aflatoxin B1 Antioxidants Curcumin Endoplasmic reticulum stress H2O2 Intestine microbiota Intestinekidney axis Sheep
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Contamination of aflatoxin B1,deoxynivalenol and zearalenone in feeds in China from 2021 to 2024 认领 引用 被引量：5
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作者 Meng Liu Zhiyuan Xia +8 位作者 Yu Zhang Rengui Yang Weicai Luo Lijia Guo Ying Liu Dessalegn Lamesgen Hua Sun Jiangfeng He Lvhui Sun 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第5期2255-2266,共12页
Background This study was carried out to investigate the individual and combined contamination of aflatoxin B1(AFB1),deoxynivalenol(DON),and zearalenone(ZEN)in feeds in China between 2021 and 2024.A total of 23,... Background This study was carried out to investigate the individual and combined contamination of aflatoxin B1(AFB1),deoxynivalenol(DON),and zearalenone(ZEN)in feeds in China between 2021 and 2024.A total of 23,003 feed samples,including 17,489 feedstuff samples and 5,514 complete feed samples,were collected from different provinces of China for mycotoxin analysis.Results The analyzed mycotoxins displayed considerably high contamination in the feed samples,with the individual contamination of AFB1,DON,and ZEN were 20.0%–100%,33.3%–100%,and 85.0%–100%,respectively.The average concentrations of AFB1,DON,and ZEN were 1.2–728.7μg/kg,106–8,634.8μg/kg,and 18.1–3,341.6μg/kg,respectively.Notably,the rates over China’s safety standards for AFB1,DON,and ZEN in raw ingredients were 9.7%,2.7%,and 15.7%,respectively.Meanwhile,3.5%,1.1%,and 8.7%of analyzed complete feeds exceeded China’s safety standards for AFB1,DON,and ZEN,respectively.Moreover,the co-contamination rates of AFB1,DON,and ZEN in more than 70%of raw ingredients and 87.5%of complete feed products were 60.0%–100%and 61.5%–100%,respectively.Conclusion This study reveals that the feeds in China have commonly been contaminated with AFB1,DON,and ZEN alone and their combination during the past four years.These findings highlight the significance of monitoring mycotoxin contaminant levels in domestic animal feed and the importance of carrying out feed administration and remediation strategies for mycotoxin control. 展开更多
关键词 Aflatoxin B1 China Contamination Deoxynivalenol Feeds Zearalenone
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Smartphone-assisted electrochemiluminescence imaging test strips towards dual-signal visualized and sensitive monitoring of aflatoxin B1 in corn samples 认领 引用 被引量：3
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作者 Miao-Miao Chen Min-Ling Zhang +5 位作者 Xiao Song Jun Jiang Xiaoqian Tang Qi Zhang Xiuhua Zhang Peiwu Li 《Chinese Chemical Letters》 SCIE CAS CSCD 2025年第1期464-468,共5页
Aflatoxins B1(AFB1)contamination in agro-food holds great threaten to human and animal health.Conventional test strips for rapid AFB1 visualized monitoring remains challenged by improvement of sensitivity and matrix i... Aflatoxins B1(AFB1)contamination in agro-food holds great threaten to human and animal health.Conventional test strips for rapid AFB1 visualized monitoring remains challenged by improvement of sensitivity and matrix interference resistance.In this case,we developed a portable electrochemiluminescence(ECL)imaging test strip with dual-signal outputs for AFB1 quantification in corn samples.RuPEI@SiO2@Au nanospheres were synthesized for bonding with anti-AFB1 antibody and then colorimetrical signal-reported on test line through the capillary flow at strips.Meanwhile,ECL imaging signal of the constructed carbon-ink-based working electrode on polyvinyl chloride substrate of strips was exported under an applied potential of 1.25 V.The whole ECL test strips not only endowed convenient colorimetric responses but guaranteed quick-witted ECL image distinguishment even at extremely low AFB1 content.The detection limit of this ECL imaging-integrated mode was 10-fold lower than that of only colorimetric mode.Furthermore,satisfactory selectivity,reliability and practicability of the as-proposed ECL test strips were demonstrated.This work offered a promising platform for on-site,accurate and sensitive detection of pollutants in foods. 展开更多
关键词 Electrochemiluminescence imaging Test strips Nanomaterials Dual-signal outputs Aflatoxin B1 Food safety
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The ameliorative role of phlorotannin on aflatoxin B1‑induced liver oxidative stress and mitochondrial injury is related to the activation of Nrf2 and Nrf1 signaling pathways in broilers 认领 引用 被引量：3
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作者 Xueqing Ye Yuying Yang +4 位作者 Qinghua Yao Mengyi Huang Balamuralikrishnan Balasubramanian Rajesh Jha Wenchao Liu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第5期2195-2212,共18页
Background Aflatoxin B1(AFB1)risks animal and human health,and the liver is considered the most crucial detoxification organ.Phlorotannin(PT)is a polyhydroxy phenol that has a wide range of biological activities... Background Aflatoxin B1(AFB1)risks animal and human health,and the liver is considered the most crucial detoxification organ.Phlorotannin(PT)is a polyhydroxy phenol that has a wide range of biological activities,including antioxidation and hepatoprotection,which can promote the ability of liver detoxification.This study aimed to elucidate the protective effect of PT on AFB1-induced liver damage in broilers.Results In vivo experiment showed that the PT reduced AFB1 content and AFB1-exo-8,9-epoxide DNA(AFBODNA)concentration in serum and liver(P<0.05),improved the histomorphology of liver and hepatic mitochondria,and activated nuclear factor erythroid 2-related factor 2(Nrf2)-related antioxidant and detoxification pathway by upregulating the activities of antioxidant enzymes(catalase[CAT],glutathione S-transferase[GST])and total antioxidant capacity(T-AOC)level(P<0.05),and inhibited the mRNA expression of CYP1A1(cytochrome P450 family 1 subfamily A member 1)and phase Ⅱ detoxification enzyme related genes(GPX1,GSTT1,and NQO1)of broilers exposed to AFB1(P<0.05).Meanwhile,PT upregulated the Nrf1 pathway-related mitochondrial biosynthetic genes(Nrf1,mitochondrial transcription factor A[TFAM],mitofusin 1[MFN1])in broilers fed AFB1 contaminated diet(P<0.05).In vitro verification study suggested that the use of Nrf2/Nrf1 inhibitors suppressed the ameliorative role of PT on AFB1-induced liver injury of broilers,which was manifested in the mRNA expression of Nrf2,NQO1,GSTT3,Nrf1,TFAM,and other genes decreasing(P<0.05),and down-regulation of the protein expression of Nrf2,total and nucleus p-Nrf2,and total and nucleus p-Nrf1(P<0.05).Conclusion The PT ameliorates oxidative stress and hepatotoxicity by activating the Nrf2-mediated phase Ⅱ detoxification enzymes pathway and maintains mitochondrial homeostasis by activating the Nrf1 signaling pathway in broilers exposed to AFB1. 展开更多
关键词 Aflatoxin B1 Biological detoxification Broiler chickens Liver injury Phlorotannin
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Gut microbiota-mediated bile acid transformations regulate the transport of aflatoxin B1 from the intestine to the liver in piglets 认领 引用 被引量：2
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作者 Jiangdi Mao Yusen Wei +3 位作者 Zhixiang Ni Jinzhi Zhang Junli Zhu Haifeng Wang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第4期1732-1749,共18页
Background Aflatoxins have been reported as a significant pollutant in feed,capable of causing harm to the liver,gastrointestinal tract and kidneys of piglets.However,research on the interactions among aflatoxin B1(AF... Background Aflatoxins have been reported as a significant pollutant in feed,capable of causing harm to the liver,gastrointestinal tract and kidneys of piglets.However,research on the interactions among aflatoxin B1(AFB1),bile acid(BA)metabolism and gut microbiota is limited.Methods In this study,piglets were treated with AFB1 and antibiotics(ABX)to evaluate the interaction between AFB1 and gut microbiota.Subsequently,the roles of the farnesoid X receptor(FXR)and sterol 12α-hydroxylase(CYP8B1)in AFB1 absorption were studied by using FXR agonists obeticholic acid(OCA)and Cyp8b1-knockout(KO)mice,respectively.Result AFB1 inhibited bile salt hydrolase(BSH)activity in ileal microbiota,downregulated ileal FXR expression,and upregulated CYP8B1 expression in liver,increasing the proportion of 12α-OH BAs and potentially enhancing AFB1 absorption.ABX treatment reduced AFB1 absorption and liver damage,and unexpectedly increased BSH activity,counteracting the AFB1-induced downregulation of FXR and upregulation of CYP8B1.OCA reactivated ileal FXR,reduced AFB1 absorption,and alleviated liver damage.Furthermore,Cyp8b1-KO mice showed increased resistance to AFB1-induced liver damage by lowering AFB1 absorption.Conclusions These results underscore the significance of gut microbiota and BAs in AFB1 absorption,suggesting new strategies to mitigate health risks from AFB1 in piglets. 展开更多
关键词 Aflatoxin Bile acid CYP8B1 FXR Microbiota Piglet
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Bifidobacterium breve alleviates gut-liver-axis injury caused by high-fat diet and aflatoxin B_1 in mice 认领 引用
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作者 Yongli Ye Tingwei Wang +3 位作者 Jian Ji Jia-Sheng Wang Yinzhi Zhang Xiulan Sun 《Food Science and Human Wellness》 SCIE CAS CSCD 2025年第2期617-632,共16页
Aflatoxin B_1(AFB_1)is a common contaminant in cereals of global concern,and long-term low-dose exposure can adversely affect human health.Here,we showed that populations with dietary patterns characterized by high-fa... Aflatoxin B_1(AFB_1)is a common contaminant in cereals of global concern,and long-term low-dose exposure can adversely affect human health.Here,we showed that populations with dietary patterns characterized by high-fat diet(HFD)might have an increased risk of exposure to high levels of AFB_1.Our data indicated that chronic exposure of AFB_1 induced“gut-liver axis”injury in mice under HFD and normal diet(ND)patterns.AFB_1 further aggravated hepatic and intestinal injury,and intestinal microbiota disruption in HFD mice.Bifidobacterium breve BAA-2849 intervention analysis showed that liver injury and lipid disorders caused by AFB_1 exposure were alleviated by regulating the proportions of different gut microbes.We demonstrated through a mice model that the populations with a dietary pattern of HFD might be more susceptible to AFB_1 exposure and adverse effects on the gut-liver axis,and the toxicity of AFB_1 exposure can be alleviated by regulating the gut microbiota. 展开更多
关键词 Aflatoxin B_1 High-fat diet Lipid metabolism Gut-liver axis Gut microbiota Bifidobacterium breve
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Five glutathione S-transferase isozymes played crucial role in the detoxification of aflatoxin B1 in chicken liver 认领 引用 被引量：1
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作者 Jiang Deng Zhe Peng +5 位作者 Zhiyuan Xia Yixin Mo Lijia Guo Jintao Wei Lvhui Sun Meng Liu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第4期1530-1541,共12页
Background AFB1-8,9-exo-epoxide(AFBO)is the highly toxic product of Aflatoxin B1(AFB1).Glutathione S-transferases(GSTs)play pivotal roles in detoxifying AFB1 by catalyzing the conjugation of AFBO with glut... Background AFB1-8,9-exo-epoxide(AFBO)is the highly toxic product of Aflatoxin B1(AFB1).Glutathione S-transferases(GSTs)play pivotal roles in detoxifying AFB1 by catalyzing the conjugation of AFBO with glutathione(GSH).Although there are over 20 GST isozymes that have been identified in chicken,GST isozymes involved in the detoxification process of AFB1 have not been identified yet.The objective of this study was to determine which GST isozymes played key role in detoxification of AFB1.Results A total of 17 pcDNA3.1(+)-GST isozyme plasmids were constructed and the GST isozyme genes were overexpressed by 80–2,500,000 folds in the chicken Leghorn male hepatoma(LMH)cells.Compared to the AFB1 treatment,overexpression of GSTA2X,GSTA3,GSTT1L,GSTZ1-1,and GSTZ1-2 increased the cell viability by 6.5%–17.0%in LMH cells.Moreover,overexpression of five GST isozymes reduced the release of lactate dehydrogenase and reactive oxygen species by 8.8%–64.4%,and 57.2%–77.6%,respectively,as well as enhanced the production AFBO-GSH by 15.8%–19.6%,thus mitigating DNA damage induced by AFB1.After comprehensive evaluation of various indicators,GSTA2X displayed the best detoxification effects against AFB1.GSTA2X was expressed in Pichia pastoris X-33 and its enzymatic properties for catalyzing the conjugation of AFBO with GSH showed that the optimum temperature and pH were 20–25℃ and 7.6–8.6 as well as the enzymatic kinetic parameter Vmax was 0.23 nmol/min/mg and the Michaelis constant was 86.05μmol/L with the AFB1 as substrate.Conclusions In conclusion,GSTA2X,GSTA3,GSTT1L,GSTZ1-1,and GSTZ1-2 played key roles in AFB1 detoxification,which will provide new remediation strategies to prevent aflatoxicosis in chickens. 展开更多
关键词 Aflatoxin B1 Chicken Detoxification Enzymatic characteristics GST isozymes
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Determination of Fungal Species to Investigate the Aflatoxin Contamination in Rice(Oryza sativa L.) 认领 引用 被引量：1
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作者 Eman Alhomaidi Aisha Umar +5 位作者 Mustansar Mubeen Laurent Dufossé Yasir Iftikhar Arpita Das Soumya Ghosh Muhammad Sibt-e-Abbas 《Phyton-International Journal of Experimental Botany》 SCIE 2025年第2期407-420,共14页
Aspergillus species produce aflatoxins and raise concerns about food safety in departmental stores and manufacturing mills.To address the risks posed by aflatoxins,and to advise the public on the highest quality rice ... Aspergillus species produce aflatoxins and raise concerns about food safety in departmental stores and manufacturing mills.To address the risks posed by aflatoxins,and to advise the public on the highest quality rice that serves as a nutritious food source,an inquiry following the guidelines outlined in both local and international standards of food safety for the presence of aflatoxins is an essential requirement.Therefore,16 white rice samples were selected randomly from low/high socio-economic departmental stores from 16 different localities.Grind powdered rice filtrate was extracted using chloroform.The filtrate applied on TLC plates and the amount of aflatoxin and moisture contents were determined.In the non-infected rice,moisture content was low(9.08%)whereas high[13.65%>12%(standard>value)]in infected ones.Four out of 8 samples of low-quality rice were contaminated with AFB1 and AFB2(ranging from 22.2 to 29.3μg/kg).All the samples except one(22.3μg/kg)from high-quality rice were certified fit despite the contamination with AFB1.Furthermore,phylogenetic analysis showed Aspergillus flavus from unfit low(Long grain brown and Brown basmati)and high-quality(Basmati-198)rice whereas A.parasiticus from unfit low-quality Medium-grain brown rice.The presented research proves that the detection of fungi and aflatoxins in rice grains poses a huge risk to the health of consumers.Therefore,it is necessary to check the rice grains before distribution. 展开更多
关键词 Mycotoxin aflatoxin rice contamination TLC principal component analysis
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HPTLC-fluorescent densitometry for screening aflatoxin B1 in millet and buckwheat 认领 引用
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作者 Xudong Shi Xingjun Xi +5 位作者 Yuetao Jia Zhijian Wang Jiawei Guo Shiyao Wang Xiaoqian Tang Yisheng Chen 《Food Science and Human Wellness》 SCIE CAS CSCD 2025年第5期1697-1702,共6页
Given severe health-hazardous effects of aflatoxin B1(AFB1) widely occurring in cereal grains and animal feeds,it is highly urgent to develop analytical methods for its rapid screening.In this work,we proposed a simpl... Given severe health-hazardous effects of aflatoxin B1(AFB1) widely occurring in cereal grains and animal feeds,it is highly urgent to develop analytical methods for its rapid screening.In this work,we proposed a simple and high-throughput method for the determination of AFB1 in millet and buckwheat samples using high performance thin layer chromatography(HPTLC) linked to fluorescence densitometry.The first step was to optimize the solid-liquid extraction for the crude clean-up of the samples.The QuEChERS(Quick,Easy,Cheap,Effective,Robust and Safe) extraction strategy was used and different solvent systems for their extraction efficiency of AFB1 from the samples were evaluated.Then,trichloromethane:ethyl acetate(7:3,V/V) was used as the mobile phase to realize the separation of the targeted compound from background noises on silica gel plates.Quantification was readily performed with densitometry in fluorescence mode.In order to fix the optimal excitation wavelength,spectra scanning ranging 250-400 nm was carried out,revealing that 364 nm light gave the highest signal.With the optimized optical system,high sensitivity to AFB1 was achieved,with a limit of detection(LOD) at 3 μg/kg.Apart from that,good linearity(0.999) was obtained within the range of 1-80 ng/band of AFB 1.To assess the analysis accuracy,2 levels of AFB 1(50 and100 μg/kg) were spiked into real grain samples.The obtained results showed that the recovery rates were within the range of 81.6%-114.0%.The proof-of-concept results of this work evidenced that HPTLC is a promising analytical tool for the screening of mycotoxin in difficult samples. 展开更多
关键词 Aflatoxin B1 High performance thin layer chromatography(HPTLC) Screening Millet Buckwheat
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Aflatoxin B1 contamination level detection in almond kernels through short wave infrared hyperspectral image analysis 认领 引用
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作者 Md.Ahasan Kabir Ivan Lee +3 位作者 Chandra B.Singh Gayatri Mishra Brajesh Kumar Panda Sang-Heon Lee 《Advanced Agrochem》 CAS CSCD 2025年第4期363-372,共10页
Aflatoxin B1(AFB1)is a toxic fungal metabolite that contaminates almonds from cultivation to harvesting.It leads to chronic health problems and significant economic loss to the producers.Therefore,a fast and non-invas... Aflatoxin B1(AFB1)is a toxic fungal metabolite that contaminates almonds from cultivation to harvesting.It leads to chronic health problems and significant economic loss to the producers.Therefore,a fast and non-invasive detection technique is crucial for safeguarding food safety by swiftly identifying and eliminating contaminated almonds from the supply chain.Hyperspectral imaging has been explored as a potential non-destructive technology for detecting AFB1.However,the diverse geometries of almonds present a significant challenge on acquired images,thereby impacting the accuracy of the developed prediction and classification models.This study investigates the effectiveness of short-wave infrared(SwIR)hyperspectral imaging combined with deep learning for detecting AFB1 in almonds of varying geometries.Initially,partial least squares regression(PLSR)and support vector machine(SvM)regression models were evaluated for quantification,while SVM and quadratic discriminant analysis(QDA)classifiers were applied for classification.The results indicated that spectral responses varied with almond thickness,making quantification models unreliable for industrial applications.The Competitive Adaptive Reweighted Sampling(CARS)algorithm was employed to identify key spectral features for developing multi-spectral AFB1 classification models to evaluate the feasibility of high-speed,accurate in-line detection.The deep learning approach significantly outperformed traditional machine learning models,with the pre-trained Inception V3 network achieving a cross-validation accuracy of 84.82%,an F1-score of 0.8522,and an area under curve of 0.893.These findings highlight the superiority of deep learning-based hyperspectral imaging for accurate and reliable AFB1 detection in almonds with diverse shapes and thicknesses. 展开更多
关键词 Aflatoxin B1 Almond thickness impact SWIR hyperspectral imaging Inline detection Non-destructive testing
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An innovative indirect competitive immunosorbent approach for precise detetion aflatoxin B1 based on high-affinity immunoglobulin G coupled CuO-anchored Fe3O4nanozymes 认领 引用
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作者 Xueqin Wang Chuan Liu +4 位作者 Haizhen Tao Xuanping Zhao Mengna Wan Junyan Liu Na Li 《Food Science and Human Wellness》 SCIE CAS CSCD 2025年第3期1017-1025,共9页
Aflatoxin B1(AFB1)is a carcinogenic toxin naturally produced in most food crops that severely threaten human health,and effective methods are urgent to improve the detection accuracy.Herein an indirect competitive imm... Aflatoxin B1(AFB1)is a carcinogenic toxin naturally produced in most food crops that severely threaten human health,and effective methods are urgent to improve the detection accuracy.Herein an indirect competitive immunosorbent approach was elaborately developed based on high-affinity immunoglobulin G(IgG)coupled CuO-anchored Fe3O4nanozymes for precise and ultrasensitive detection of AFB1in food crops including peanut,corn and wheat.The high-affinity nanozymes were fabricated by the assembly of inner core Fe3O4nanoparticles and mesoporous silica capping layer,Cu O further situated within large aperture of the coating layer via in-situ growth,and then conjugated with ligand rabbit anti-mouse Ig G,which can specifically bind with AFB1.The results showed the hybrid high-affinity nanozymes displayed enhanced peroxidasemimic activities and catalytic performances,achieving a linear range of 0.06-61.93(lg(ng/mL))and a detection limit of 0.0037 ng/mL,30 times better than that of the conventional enzyme-linked immunosorbent assay.The constructed nanozymes were successfully applied to the detection of AFB1in food products with an average spiked recovery of 96.53%and relative standard deviations less than 2.8%.Therefore,the accurate hybrid nanozymes may serve for AFB1detection in various foods in future. 展开更多
关键词 Aflatoxin B1 Enhanced peroxidase-mimic activities Nanozyme based enzyme-linked immunosorbent assay Food crops
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Empowering innovative strategies:Utilizing polymer-based nanotechnology for the prevention,control,and detection of aflatoxins,ochratoxins,and fusarium toxins in food systems 认领 引用
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作者 Sanduni Dabare Sisitha Rajapaksha Imalka Munaweera 《Grain & Oil Science and Technology》 EI CAS 2025年第4期318-334,共17页
Mycotoxins,toxic secondary metabolites produced by fungus including Aspergillus,Penicillium,and Fusarium,pose considerable threats to food safety and human health worldwide.This review analyzes the main categories of ... Mycotoxins,toxic secondary metabolites produced by fungus including Aspergillus,Penicillium,and Fusarium,pose considerable threats to food safety and human health worldwide.This review analyzes the main categories of mycotoxins—namely aflatoxins,ochratoxins,and fusarium toxins(zearalenone and fumonisins)—along with their health implications,sources of contamination,and environmental circumstances conducive to their production.The document highlights the pressing necessity for efficient management techniques and investigates the use of food polymer-based nanotechnology as an innovative solution.Biopolymeric nanoparticles produced from natural food materials exhibit notable antibacterial characteristics,biodegradability,and the ability to enhance mycotoxin detection and management.This review emphasizes the transformative capacity of nanotechnology based innovative strategies in improving mycotoxin control,providing insights into emerging research avenues and practical applications to bolster food safety systems and keyword co-occurrence analysis,limitations and future perspectives. 展开更多
关键词 Aflatoxin Ochratoxin Fusarium toxins Toxins prevention Polymer Nanotechnology
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Mechanistic interplay between aflatoxin B1 and tissue inhibitor of metalloproteinase-3 in hepatocellular carcinoma 认领 引用
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作者 Yan Luo Li-Juan Wang Cheng-Long Wang 《World Journal of Hepatology》 2025年第10期1-7,共7页
Hepatocellular carcinoma(HCC)is a major global health challenge,particularly in regions with high aflatoxin B1(AFB1)exposure.This editorial explores the mechanistic interplay between AFB1 and tissue inhibitor of metal... Hepatocellular carcinoma(HCC)is a major global health challenge,particularly in regions with high aflatoxin B1(AFB1)exposure.This editorial explores the mechanistic interplay between AFB1 and tissue inhibitor of metalloproteinase-3(TIMP-3)in AFB1-related HCC.TIMP-3,frequently downregulated in HCC due to promoter methylation,is linked to increased tumor aggressiveness and poor prognosis.We propose that AFB1 induces epigenetic silencing of TIMP-3,potentially via DNA adducts and oxidative stress,exacerbating AFB1-related HCC progression.This AFB1-TIMP-3 axis highlights TIMP-3’s potential as a prognostic biomarker and therapeutic target.Future research should focus on elucidating these molecular pathways and integrating TIMP-3 into clinical practice for early detection and targeted therapies in AFB1-prevalent regions. 展开更多
关键词 Hepatocellular carcinoma Tissue inhibitor of metalloproteinase-3 Aflatoxin B1 Prognosis Biomarker
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Biocontrol potential of reducing aflatoxin contamination by an atoxigenic strain of Aspergillus flavus. isolated from peanut 认领 引用 被引量：2
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作者 Liying YAN Yanping KANG +5 位作者 Yong LEI Liyun WAN Dongxin HUAI Huifang JIANG Xiaoping REN Boshou LIAO 《Oil Crop Science》 2016年第1期45-51,共7页
An Aspergillus section/Zam isolate （ NAFFHB396） was isolated from a peanut kernel. It was identified as Aspergillus flavus based on morphology and molecular characteristics. It produced yellow to green - colored con... An Aspergillus section/Zam isolate （ NAFFHB396） was isolated from a peanut kernel. It was identified as Aspergillus flavus based on morphology and molecular characteristics. It produced yellow to green - colored conidia on CYA medium and colonies with bright orange in color on AFPA medium. NAFFHB396 was grouped with A. flavus NRRL21882 and NRRL3357 by phylogenetic analysis of partial calmodulin sequence data. It was found that 12 genes were absent in aflatoxin gene cluster in NAFF- HB396. HPLC result further showed that it was an atoxigenic isolate. Co - inoculation of NAFFHB396 with a high aflatoxin producer AF2202 at the ratio of 1：1 both on CYA medium and peanut kernel resul-ted in reduction of aflatoxin production by 88.7% and 99. 8% respectively. These results suggested that the atoxigenic NAFFHB396 obtained in this study had a great potential to be a biocontrol agent to reduce aflatoxin contamination of peanut in China. 展开更多
关键词 peanut aflatoxin atoxigenic Aspergillus flavus aflatoxin gene cluster biocontrol
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Occurrence of Aflatoxin B1,deoxynivalenol and zearalenone in feeds in China during 2018-2020 认领 引用 被引量：44
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作者 Ling Zhao Lei Zhang +5 位作者 Zijian Xu Xingda Liu Liyuan Chen Jiefan Dai Niel Alexander Karrow Lvhui Sun 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2022年第1期197-208,共12页
Background:The current study was conducted to investigate the individual and combined occurrence of aflatoxin B1(AFB1),deoxynivalenol(DON)and zearalenone(ZEN)in feeds from various Provinces of China during 2018 ... Background:The current study was conducted to investigate the individual and combined occurrence of aflatoxin B1(AFB1),deoxynivalenol(DON)and zearalenone(ZEN)in feeds from various Provinces of China during 2018 to 2020.A total of 3,507 feed samples,including 2,090 feed ingredients and 1,417 complete feed samples,were collected from different areas of China for mycotoxins analysis.Results:The individual contamination of AFB1,DON and ZEN were present in more than 81.9%,96.4% and 96.9% of feed samples,respectively,with average concentration ranges of AFB1 between 1.2-27.4μg/kg,DON between 458.0-1,925.4μg/kg and ZEN between 48.1-326.8μg/kg.Notably,0.9%,0.5% and 0.1% of feed ingredients,and 1.2-12.8%,0.9-2.9% and 0-8.9% of complete feeds for pigs,poultry and ruminants with AFB1,ZEN and DON that exceeded China’s safety standards,respectively.Moreover,more than 81.5%of feed ingredients and 95.7% of complete feeds were co-contaminated with various combinations of these mycotoxins.Conclusion:This study indicates that the feeds in China were universally contaminated with AFB1,DON and ZEN during the past 3 years.These findings highlight the significance of monitoring mycotoxin contaminant levels in the domestic animal feed,and the importance of carrying out feed administration and remediation strategies for mycotoxin control. 展开更多
关键词 Aflatoxin B1 China Deoxynivalenol Feeds Zearalenone
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Hepatitis B virus x gene and cyanobacterial toxins promote aflatoxin B_1-induced hepatotumorigenesis in mice 认领 引用 被引量：15
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作者 Min Lian Ying Liu +3 位作者 Shun-Zhang Yu Geng-Sun Qian Shu-Guang Wan Kenneth R Dixon 《World Journal of Gastroenterology》 SCIE CAS 2006年第19期3065-3072,共8页
AIM： To assess the combinative role of aflatoxin B1 （AFB1）, cyanobacterial toxins （cyanotoxins）, and hepatitis B virus （HBV） x gene in hepatotumorigenicity. METHODS： One-week-old animals carrying HBV x gene an... AIM： To assess the combinative role of aflatoxin B1 （AFB1）, cyanobacterial toxins （cyanotoxins）, and hepatitis B virus （HBV） x gene in hepatotumorigenicity. METHODS： One-week-old animals carrying HBV x gene and their wild-type littermates were intraperitoneally （ip） injected with either single-dose AFB1 [6 mg/kg body weight （bw）], repeated-dose cyanotoxins （microcystin- LR or nodularin, 10 μg/kg bw once a week for 15 wk）, DMSO （vehicle control） alone, or AFB1 followed by cyanotoxins a week later, and were sacrificed at 24 and 52 wk post-treatment. RESULTS： AFB1 induced liver tumors in 13 of 29 （44.8%） transcjenic mice at 52 wk post-treatment, significantly more frequent than in wild-type mice （13.3%）. This significant difference was not shown in the 24-wk study. Compared with AFB1 exposure alone, MC-LR and nodularin yielded approximately 3-fold and 6-fold increases in the incidence of AFB1-induced liver tumors in wild-type animals at 24 wk, respectively. HBV x gene did not further elevate the risk associated with coexposure to AFB1 and cyanotoxins. With the exception of an MC-LR-dosed wild-type mouse, no liver tumor was observed in mice treated with cyanotoxins alone at 24 wk. Neither DMSO-treated transgenic mice nor their wild-type littermates had pathologic alterations relevant to hepatotumorigenesis in even up to 52 wk. CONCLUSION： HBV x gene and nodularin promote the development of AFB1-induced liver tumors. Co-exposure to AFB1 and MC-LR tends to elevate the risk of liver tumors at 24 wk relative to exposure to one of them. The combinative effect of AFB1, cyanotoxins and HBVx on hepatotumorigenesis is weak at 24 wk. 展开更多
关键词 Aflatoxins Cyanobacteria Hepatitis B virus Liver neoplasms Transgenic mice
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Biological control of aflatoxin contamination of crops 认领 引用 被引量：15
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作者 Yan-ni YIN Lei-yan YAN +1 位作者 Jin-hua JIANG Zhong-hua MA 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS 2008年第10期787-792,共6页
Aflatoxins produced primarily by two closely related fungi,Aspergillus flavus and Aspergillus parasiticus,are mutagenic and carcinogenic in animals and humans.Of many approaches investigated to manage aflatoxin contam... Aflatoxins produced primarily by two closely related fungi,Aspergillus flavus and Aspergillus parasiticus,are mutagenic and carcinogenic in animals and humans.Of many approaches investigated to manage aflatoxin contamination,bio-logical control method has shown great promise.Numerous organisms,including bacteria,yeasts and nontoxigenic fungal strains of A.flavus and A.parasiticus,have been tested for their ability in controlling aflatoxin contamination.Great successes in reducing aflatoxin contamination have been achieved by application of nontoxigenic strains of A.flavus and A.parasiticus in fields of cotton,peanut,maize and pistachio.The nontoxigenic strains applied to soil occupy the same niches as the natural occurring toxigenic strains.They,therefore,are capable of competing and displacing toxigenic strains.In this paper,we review recent development in biological control of aflatoxin contamination. 展开更多
关键词 Aflatoxin Aspergillus Biocontrol Food safety
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The Development of A Fluorescence Polarization Immunoassay for Aflatoxin Detection 认领 引用 被引量：13
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作者 SHENG Ya Jie EREMIN Sergei +3 位作者 MI Tie Jun ZHANG Su Xia SHEN Jian Zhong WANG Zhan Hui 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2014年第2期126-129,共4页
A fluorescence polarization immunoassay （FPIA） was developed for the analysis ofaflatoxins （AFs） using an anti-aflatoxin B1 （AFB1） monoclonal antibody and a novel fluorescein-labeled AFB1 tracer. The FPIA showed... A fluorescence polarization immunoassay （FPIA） was developed for the analysis ofaflatoxins （AFs） using an anti-aflatoxin B1 （AFB1） monoclonal antibody and a novel fluorescein-labeled AFB1 tracer. The FPIA showed an IC50 value of 23.33 ng/mL with a limit of detection of 13.12 ng/mL for AFB1. The cross-reactivities of AFB1, AFB2, AFG1, AFG2, AFM1, and AFM2 with the antibody were 100%, 65.7%, 143%, 23.5%, 111.4%, and 2%, respectively. The group-specificity of anti-AFB1mAb indicated that the FPIA could potentially be used in a screening method for the detection of total AFs, albeit not AFG2 and AFM2. The total time required for analyzing 96 samples in one microplate was less than 5 rain. This study demonstrates the potential usefulness of the FPIA as a rapid and simple technique for monitoring AFs. 展开更多
关键词 FPIA AFB The Development of A Fluorescence Polarization Immunoassay for Aflatoxin Detection AFM EDF
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A simple aptamer molecular beacon assay for rapid detection of aflatoxin B1 认领 引用 被引量：12
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作者 Chao Wang Linlin Sun Qiang Zhao 《Chinese Chemical Letters》 SCIE CAS CSCD 2019年第5期1017-1020,共4页
Aflatoxin B1(AFB1)is a highly toxic mycotoxin,and rapid and sensitive detection of AFB1 is in demand for food safety and environmental analysis.Here we described a simple aptamer molecular beacon assay for rapid detec... Aflatoxin B1(AFB1)is a highly toxic mycotoxin,and rapid and sensitive detection of AFB1 is in demand for food safety and environmental analysis.Here we described a simple aptamer molecular beacon assay for rapid detection of aflatoxin B1(AFB1)by using an aptamer with a fluorescein(FAM)label at the 50 end and a fluorescence quencher(black hole quencher 1,BHQ1)at the 30 end.In the presence of AFB1,the aptamer probe bound with AFB1 and induced a hairpin structure,drawing FAM and BHQ1 into close proximity and leading to fluorescence quenching.This assay allowed for a detection limit of 3.9 nmol/L and a dynamic range from 3.9 nmol/L to 4 mmol/L.Specificity test showed other mycotoxins including ochratoxin A,ochratoxin B,fumonisin B1,fumonisin B2,and zearalenone had negligible influence on detection of AFB1.AFB1 spiked in diluted liquor wine,methanol,or corn flour samples was successfully detected by using this aptamer probe,and the assay showed potential for real sample analysis. 展开更多
关键词 Aptamer Aflatoxin B1 Fluorescence Molecular beacons Mycotoxin Sensor
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