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Redox-neuroimmune Correlations of VHH Expression Under Varying Protein Nutritions in Alpacas 认领 引用
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作者 Chen Zhihui Chai Haoliang +3 位作者 Zhao Dexin Yu Xilong Xu Liangmei Ma Deying 《Journal of Northeast Agricultural University(English Edition)》 CAS 2026年第2期39-48,共10页
This study reanalyzed serum biomarkers from alpacas fed diets differing in protein levels.Twenty-eight indicators,including oxidative,neuroendocrine and immune markers,were analyzed using Z-score normalization,correla... This study reanalyzed serum biomarkers from alpacas fed diets differing in protein levels.Twenty-eight indicators,including oxidative,neuroendocrine and immune markers,were analyzed using Z-score normalization,correlation and principal component analysis(PCA).Results indicated that high-protein diets enhanced antioxidant enzymes and immune markers,while low-protein diets elevated stress hormones.Correlations revealed a positive antioxidant-immune axis and a negative stress-immune axis.Variable domain of heavy-chain-only antibody(VHH)expression closely aligned with redox and neuroimmune balance,independent of transient inflammation.These findings identified VHH as a stable,protein-sensitive biomarker of immune function in alpacas. 展开更多
关键词 alpaca dietary protein VHH multivariate analysis correlation nutritional immunology
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Microbial Community in the Forestomachs of Alpacas (Lama pacos) and Sheep (Ovis aries) 认领 引用 被引量：3
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作者 PEI Cai-xia LIU Qiang +3 位作者 DONG Chang-sheng LI Hong-quan JIANG Jun-bing GAO Wen-jun 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2013年第2期314-318,共5页
Four 2-yr old alpacas （（48±2.3） kg） and four 2-yr old sheep （（50±1.7） kg） were used to study the pH and microbial community of forestomach from alpacas （Lama pacos） and sheep （Ovis aries） fed fre... Four 2-yr old alpacas （（48±2.3） kg） and four 2-yr old sheep （（50±1.7） kg） were used to study the pH and microbial community of forestomach from alpacas （Lama pacos） and sheep （Ovis aries） fed fresh alfalfa as the sole forage at low altitude （793 m）. The forestomach fluid was taken anaerobically via the esophagus. The electric pH meter and quantitative polymerase chain reaction systems were used to study the the pH and microbial community of forestomach. The results showed that the mean pH of forestomach fluid from alpacas was higher than that from sheep （P〈0.01）. The percentages of methanogens and Ruminococcusflavefaciens to total bacterial were lower in the forestomach of alpacas than that in the rumen of sheep, while the percentage of fungi and Fibrobacter succinogenes were higher. The percentage of protozoa was similar in the forestomach of alpacas and sheep. These differences can partly explain the reason that alpacas were lower methane production than sheep. 展开更多
关键词 forestomach microbial community alpacas sheep alfalfa
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Molecular Identification of Shiga-Toxin Producing and Enteropathogenic Escherichia coli(STEC and EPEC) in Diarrheic and Healthy Young Alpacas 认领 引用
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作者 Lorena Mori Rosa Perales +4 位作者 Jorge Rodríguez Carlos Shiva Ysabel Koga Geraldine Choquehuanca Cesar Palacios 《Advances in Microbiology》 CAS 2014年第7期360-364,共5页
Isolation and biochemical and molecular identification of 303 strains of Escherichia coli obtained from diarrheic and healthy young alpacas of Puno-Peru, were realized. PCR amplification for 7 virulence factor genes a... Isolation and biochemical and molecular identification of 303 strains of Escherichia coli obtained from diarrheic and healthy young alpacas of Puno-Peru, were realized. PCR amplification for 7 virulence factor genes associated with STEC, STEC O157:H7, EPEC: sxt1, sxt2, rfbO157, fliCH7, hlyA, eae y bfp were determined. A total of 39 strains (12.88%) showed amplification for one or more of these genes. Twenty three strains (59%) were classified as STEC and 16 strains (41%) as EPEC. An 88.18% (34/39) of STEC and EPEC strains were obtained from healthy alpacas and only 11.82% (5/39) from diarrheic alpacas considering this specie as potential zoonotic reservoir of STEC and EPEC. 展开更多
关键词 Shiga Toxin Producing Escherichia coli Enteropathogenic Escherichia coli Diarrhea Young Alpacas
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Cloning and Expression Level Analysis of Melanocyte-stimulating Hormone Receptor 1 Gene(MC1R) in Alpacas with Different Coat Color 认领 引用
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作者 REN Yu-hong REN Bin +4 位作者 FAN Rui-wen ZHU Zhi-wei YANG Yong LI Hui DONG Chang-sheng 《畜牧兽医学报》 CAS 北大核心 2010年第S1期21-25,共5页
Specific primers for the MC1R gene of alpacas(GenBank EU1358800) were designed to amplify the cDNA sequence using RT-PCR to seek variation in the sequence and explore the relationship between the expression level of M... Specific primers for the MC1R gene of alpacas(GenBank EU1358800) were designed to amplify the cDNA sequence using RT-PCR to seek variation in the sequence and explore the relationship between the expression level of MC1R gene and alpaca coat color.The MC1R gene from white alpaca was cloned successfully and sequence analysis verified that the MC1R gene,encoding 317 amino acids,was 1081 bp in length.Compared with the existing sequence in GenBank,sequence identity was 99.9%and 7 mutations were found.Primers,designed from the sequence obtained,were used to assess the relative expression of MC1R in alpacas of different coat color using QRT-PCR and SPSS 13.0 software.Relative expression of MC1R in the skin of brown alpacas was 4.32 times higher than that in white alpacas after normalization with GAPDH(P【0.01),indicating that MC1R expression may be related to coat color of alpacas. 展开更多
关键词 alpaca melanocyte-stimulating hormone receptor 1 gene(MC1R) cloning QRT-PCR gene expression level
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Cover Story 认领 引用
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《Journal of Mountain Science》 SCIE CSCD 2026年第2期F0004-F0004,共1页
The cover photograph shows a herd of alpacas(Vicugna pacos) grazing on high-altitude Andean peatlands known as bofedales in Sajama National Park(SNP),Bolivia's first protected area,established in 1939.In the backg... The cover photograph shows a herd of alpacas(Vicugna pacos) grazing on high-altitude Andean peatlands known as bofedales in Sajama National Park(SNP),Bolivia's first protected area,established in 1939.In the background,the snow-capped Nevado Sajama(6,542 m a.s.l.),Bolivia's highest peak,dominates the landscape,with Polylepis tarapacana forests—considered the highest-elevation arboreal forests in the world—visible on its slopes.The image captures the intimate relationship between pastoral fivelihoods and mountain ecosystems that has sustained Aymara communities in the western Bolivian Andes for centuries.Located between 3,960 and 6,542 m a.s.l.,the park encompasses a diverse mosaic of ecological zones including peatlands,wet meadows,shrublands,grasslands,and high-Andean forests.Photograph was taken on April 16,2021 by Rosa Isela Meneses. 展开更多
关键词 pastoral livelihoods alpacas bofedales alpacas vicugna pacos polylepis tarapacana vicugna pacos Sajama National Park high altitude ecosystems
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Emerging from the desert:Arabian camel hair 认领 引用
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作者 Zhao Xinhua 《China Textile》 2026年第2期33-35,共3页
The textile industry stands at a critical crossroads.With climate change,overgrazing,and water scarcity threatening the stability of natural-fiber supply chains,traditional "noble fibers" such as cashmere an... The textile industry stands at a critical crossroads.With climate change,overgrazing,and water scarcity threatening the stability of natural-fiber supply chains,traditional "noble fibers" such as cashmere and alpaca are becoming increasingly difficult to source ethically and sustainably,Meanwhile,cotton's high-water footprint and wool's methane emissions raise additionalenvironmental concerns.At the same time,materials and brands are losing connection with cultural roots and geographic identity,prompting renewed interest in fibers that combine authenticity with sustainability.Against this backdrop,the search for new,scalable,and responsible materials has intensified,and one overlooked resource is finally emerging from the world's deserts:Ara bian camel hair. 展开更多
关键词 overgrazing cotton cashmere water scarcity alpaca natural fiber supply chains high water footprint climate change
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Current Situation and Development Countermeasures of Alpaca Breeding 认领 引用 被引量：3
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作者 佘秋生 张红波 +2 位作者 娄鹏雁 姬晓娜 赵桢 《Agricultural Science & Technology》 CAS 2011年第5期733-736,共4页
This paper briefly introduces the biological characteristics and value of alpaca breeding,reviews the development of alpaca breeding,describes the global distribution of alpaca and the current situation of alpaca bree... This paper briefly introduces the biological characteristics and value of alpaca breeding,reviews the development of alpaca breeding,describes the global distribution of alpaca and the current situation of alpaca breeding in the introduction of well-bred,indicates the technology of feeding,managing and deep processing.And finally some countermeasures for future development of alpaca breeding in China are put forward. 展开更多
关键词 Alpaca Breeding Current situation Development countermeasures
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Effects on the quality of frozen-thawed alpaca(Lama pacos)semen using two different cryoprotectants and extenders 认领 引用 被引量：1
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作者 AlexeiSantiani WilfredoHuanca +3 位作者 RómuloSapana TeodosioHuanca NéstorSepúlveda RaúlSánchez 《Asian Journal of Andrology》 SCIE CAS 2005年第3期303-309,共7页
Aim： To evaluate two extenders and two cryoprotectant agents （CPA） for alpaca semen cryopreservation. Methods： Semen samples were obtained from four adult alpacas （Lama pacos） and frozen using extender Ⅰ （TRIS... Aim： To evaluate two extenders and two cryoprotectant agents （CPA） for alpaca semen cryopreservation. Methods： Semen samples were obtained from four adult alpacas （Lama pacos） and frozen using extender Ⅰ （TRIS, citrate, egg yolk and glucose） or extender Ⅱ （skim milk, egg yolk and fructose）, each containing either glycerol （G） or ethylene glycol （EG） as CPA. Consequently, four groups were formed： 1） extender Ⅰ-G; 2） extender Ⅰ-EG; 3） extender Ⅱ-G; and 4） extender Ⅱ-EG. Semen was diluted in a two-step process： for cooling to 5 ℃ （extenders without CPA）, and for freezing （extenders with CPA）. Viability and acrosome integrity were assessed using trypan blue and Giemsa stains. Results： When compared, the motility after thawing was higher （P 〈 0.05） in groups Ⅱ-EG （20.0 %±6.7 %） and Ⅱ-G （15.3 %±4.1%） than that in groups Ⅰ-G （4.0 %±1.1%） and Ⅰ-EG （1.0 %±1.4 %）. Viable spermatozoa with intact acrosomes in groups Ⅱ-EG （18.7 %±2.9 %） and Ⅱ-G （12.7 %±5.9 %） were higher than that in groups Ⅰ-G （5.7 %±1.5 %） and Ⅰ-EG （4.0 %±1.0 %）. Conclusion： The skim milk- and egg yolk-based extenders containing ethylene glycol or glycerol to freeze alpaca semen seems to promote the survival of more sperm cells with intact acrosomes than the other extenders. （Asian J Androl 2005 Sep; 7： 303-309） 展开更多
关键词 cryopreservation glycerol ethylene glycol extenders alpaca spermatozoa
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Ribosomal proteins expression and phylogeny in alpaca (Lama pacos) skin 认领 引用
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作者 Junming Bai Ruiwen Fan +3 位作者 Shanshan Yang Yuankai Ji Jianshan Xie Chang-Sheng Dong 《Advances in Bioscience and Biotechnology》 CAS 2012年第3期231-237,共7页
Ribosomal proteins (RP) has been reported as a central player in the translation system, and are required for the growth and maintenance of all cell kinds. RP genes form a family of homologous proteins that express in... Ribosomal proteins (RP) has been reported as a central player in the translation system, and are required for the growth and maintenance of all cell kinds. RP genes form a family of homologous proteins that express in the stable pattern and were used for phylogenetic analysis. Here we constructed a cDNA library of alpaca skin and 13,800 clones were sequenced. In the cDNA library, RP genes from skin cDNA library of alpaca were identified. Then 8 RP genes were selected at random and built the phylogenetic trees from the DNA sequences by using parsimony or maximum likelihood methods for molecular and evolutionary analysis of ribosomal proteins. The results showed that the 42 RP genes of alpaca have been expressed in alpaca skin. They were highly conserved. The phylogeny inferred from all these methods suggested that the evolutionary distances of alpaca RP genes were closer to rat. 展开更多
关键词 Ribosomal Protein Expression Phylogenetic Tree Alpaca
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