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A new method for determination of antithrombotic activity of egg white protein hydrolysate by microplate reader 认领 引用 被引量：18
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作者 Wan Gen Yang Zhang Wang Shi Ying Xu 《Chinese Chemical Letters》 SCIE CAS 2007年第4期449-451,共3页
A new method for the determination of antithrombotic activity of egg white protein hydrolysate （EWPH） was developed using a microplate reader. Reaction was carried out at 37℃and pH 7.2 with fibrinogen concentration... A new method for the determination of antithrombotic activity of egg white protein hydrolysate （EWPH） was developed using a microplate reader. Reaction was carried out at 37℃and pH 7.2 with fibrinogen concentration 0.1%. Microplate reading was conducted at 405 nm. Inhibition rate of EWPH on thrombin activity showed linearity （R2 = 0.9971）, when the inhibition rate was in the range of 10-90%. The lower limit of detection （LLD, at 99.7% probability） and the biological limit of detection （BLD, at 99.7% probability） of the method were 10.643 and 40 mg/mL, respectively. The repeatability standard deviation （R.S.D.） was 1.08%. The standard deviation of the method was ±0.027 AT-U. 展开更多
关键词 Egg white protein hydrolysate Antithrombotic activity Microplate reader
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Structural characteristics,anticoagulant and antithrombotic mechanism of a novel polysaccharide from Rosa Chinensis Flos 认领 引用 被引量：10
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作者 Xiaofeng Zhang Zhenhua Liang +5 位作者 Geoffrey Ivan Neil Waterhouse Shengjun Jiang Dongxiao Sun-Waterhouse Jinmei Wang Changyang Ma Wenyi Kang 《Food Science and Human Wellness》 SCIE CAS CSCD 2023年第2期407-415,共9页
This is the first report on a polysaccharide(RCJ2-Ib)isolated from Rosa Chinensis Flos.RCJ2-Ib was obtained through the extraction with water,precipitation with ethanol,separation with DEAE-52 column and purification ... This is the first report on a polysaccharide(RCJ2-Ib)isolated from Rosa Chinensis Flos.RCJ2-Ib was obtained through the extraction with water,precipitation with ethanol,separation with DEAE-52 column and purification with DEAE-Sepharose Fast Flow column and Sephadex G100 column.GC,FT-IR and NMR analyses revealed that RCJ2-Ib(3.3 k Da)was a 1,4-linked polymannuronic acid containing substantialβ-Danomers units.The anticoagulant effect of RCJ2-Ib evaluated by using rabbit ear venous blood and an acute blood stasis rat model showed that RCJ2-Ib had obvious anticoagulant activity in regulating endogenous and exogenous coagulation pathways and reducing serum thromboxane B2 and endothelin-1.In addition,RCJ2-Ib could also increase the number of Lactobacillus and Escherichia coli.As a result,RCJ2-Ib has the potential to inhibit thrombosis and maintain the intestinal environment. 展开更多
关键词 Rosa Chinensis Flos Polysaccharide isolation Antithrombotic activity Intestinal flora
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Physicochemical Properties and Bioactivities of Rice Beans Fermented by Bacillus amyloliquefaciens 认领 引用 被引量：8
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作者 Shan Wu Shuai Lu +3 位作者 Jun Liu Shaoqing Yang Qiaojuan Yan Zhengqiang Jiang 《Engineering》 SCIE EI CAS CSCD 2021年第2期219-225,共7页
The purpose of this study was to investigate the physicochemical properties and bioactivities of rice beans(Vigna umbellata)fermented by Bacillus amyloliquefaciens.The fermentation conditions were optimized on the bas... The purpose of this study was to investigate the physicochemical properties and bioactivities of rice beans(Vigna umbellata)fermented by Bacillus amyloliquefaciens.The fermentation conditions were optimized on the basis of the fibrinolytic activity.Under the optimal fermentation conditions,the fibrinolytic activity reached a maximum of 78.0 FU·g-1(4890 IU·g-1,fibrin plate method,FU:fibrin degradation unit).The contents of peptides(which increased from 2.1 to 10.9 g per 100 g),total phenolics(from 116.7 to 388.5 mg gallic acid per 100 g),total flavonoids(from 235.5 to 354.3 mg rutin per 100 g),and anthocyanin(from 20.1 to 47.1 mg per 100 g),as well as the superoxide dismutase activity(from 55.3 to 263.6 U·g-1)in rice beans were significantly increased after fermentation.The 2,2-diphenyl-1-picrylhydrazyl(DPPH)and 2,2-azinobis(3-ethylbenzothiazoline-6-sulphonic acid)diammonium salt(ABTS)free radical scavenging activities and ferric reducing antioxidant power(FRAP)of fermented rice beans were 1.9–4.8 times higher than those of unfermented rice beans.Moreover,fermentation induced an increase in the dipeptidyl peptidase IV(DPP-IV)inhibition,a-glucosidase inhibition,and anticoagulant activities of rice beans.Rice beans fermented by Bacillus amyloliquefaciens may serve as a functional food with potential benefits for the prevention of thrombotic diseases. 展开更多
关键词 Solid-state fermentation Rice beans Bacillus amyloliquefaciens Antithrombotic activity Antioxidant activity Antidiabetic activity
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Sesquiterpenoids from the hydrothermal vent sediment derived fungus Penicillium sp.JWM79-5-1 with antithrombotic and pro-angiogenic activities 认领 引用
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作者 Wenjuan Ding Xiaobin Li +6 位作者 Xiyue Wang Shuqing Yu Xunuo Li FangFang Wang Danmei Tian Bin Wu Jinshan Tang 《Marine Life Science & Technology》 SCIE CAS CSCD 2025年第4期901-914,共14页
Seven previously unreported sesquiterpenes including three rare 5/5/6-fused tricyclic gymnomitrane-type sesquiterpenes(1-3),an ent-longipinane-type sesquiterpene(4),a cuparane-type sesquiterpene(5),and two chamigrane-... Seven previously unreported sesquiterpenes including three rare 5/5/6-fused tricyclic gymnomitrane-type sesquiterpenes(1-3),an ent-longipinane-type sesquiterpene(4),a cuparane-type sesquiterpene(5),and two chamigrane-type sesquiterpenes(6 and 7),along with a known chamigrane-type sesquiterpene xylariterpenoid C(8)were isolated from the hydrothermal vent sediment derived fungus Penicillium sp.JWM79-5-1.Their structures were identified on the basis of 1D and 2D NMR,in conjunction with Mosher’s method,X-ray crystallography,and electronic circular dichroism(ECD).The antithrombotic activity of compounds 1-6 and 8 was evaluated in arachidonic acid(AA)-induced zebrafish thrombosis model in vivo.The results revealed compound 1 with potent antithrombotic activity in a concentration-dependent manner.Further,the pro-angi-ogenic activities of compounds 1-6 and 8 were evaluated in a transgenic zebrafish model that expresses vegfr2,the receptor for the angiogenic factor VEGF,tagged with the green fluorescence protein(vegfr2-GFP)through detecting the length of both intersegmental vessels(ISVs)and subintestinal veins(SIVs).The results revealed that compound 1 exhibited a potent antithrombotic activity while 3 revealed a potent pro-angiogenic activity.These findings strongly support drug development of these compounds in cardiovascular disease treatment. 展开更多
关键词 Marine-derived fungus Secondary metabolites Sesquiterpenes Antithrombotic activity Pro-angiogenic activity
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