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The environmental pollutants of arsenic and microplastics mediating soil microbiota shift aphid growth in tobacco 认领 引用
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作者 Yuan Zhang Mengting Wang +4 位作者 Hu Li Feng Gao Yufang Meng Jinhong Zhou Zihua Zhao 《Ecological Frontiers》 CSCD 2026年第2期728-734,共7页
Environmental pollutants are ubiquitous and persistent in soil,water,and air,posing serious threats to biodiversity,eco-logical functions,and human health.The wide application of agricultural chemicals has led to the ... Environmental pollutants are ubiquitous and persistent in soil,water,and air,posing serious threats to biodiversity,eco-logical functions,and human health.The wide application of agricultural chemicals has led to the release of large amounts of environmental pollutants,which have driven a rapid shift in ecological function via mediating microbiota composition in agroecosystems.In this study,we employed 16S rRNA amplicon sequencing,coupled with phenotype determination of tobacco(Nicotiana tabacum)and aphid(Myzus persicae),to explore the multi-trophic responses of a soil microbiome-tobacco-aphid system to arsenic(As)and microplastics(MPs)stress.Soil residues of As and MPs signif-icantly reduced the species diversity of soil microorganisms and led to a shift in function of microbial community.No-tably,the relative abundances of specific taxa,such as Gemmatimonas and Edaphobaculum,increased following As and MPs exposure,whereas Aquicella and Phycicoccus exhibited a significant decline in contrast.Furthermore,MPs increased the size of tobacco leaves and stem circumference.However,the growth rate of tobacco height significantly decreased with MPs residues in the soil.As exposure reduced the size and number of effective tobacco leaves.The behavioral ex-periment revealed that aphids were attracted to As-stressed tobacco leaves more than to the control.Interestingly,M.persicae exhibited enhanced growth and reproduction on As-stressed leaves,while showing a partially adverse re-sponse to MPs exposure.Therefore,As exposure significantly promoted aphid population growth and potential outbreak risk.Consequently,environmental pollutants affect the complex ecological network among microorganisms,plants,and insects,and pose potential threats to the structural stability and functional integrity of agricultural ecosystems. 展开更多
关键词 Arsenic Microplastics Soil microbiome Tobacco Aphid
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The complete mitochondrial pruinosa(Hemiptera:Aphididae:genome of Lambersaphis:Chaitophorinae)and phylogenetic implications 认领 引用
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作者 Cailing LI Zhangchen CHI +4 位作者 Xiaolu ZHANG Zhao PAN Liyun JIANG Gexia QIAO Jing CHEN 《Entomotaxonomia》 CSCD 2026年第2期104-116,共13页
Lambersaphis pruinosa(Narzikulov)is the only representative of the aphid genus Lambersaphis(Aphididae:Chaitophorinae).This study sequenced the complete mitochondrial genome of L.pruinosa.It possesses a total length of... Lambersaphis pruinosa(Narzikulov)is the only representative of the aphid genus Lambersaphis(Aphididae:Chaitophorinae).This study sequenced the complete mitochondrial genome of L.pruinosa.It possesses a total length of 16,939 bp,comprised of 13 protein-coding genes(PCGs),22 tRNA genes,2 rRNA genes,a control region,and a repeat region.The whole mitogenome exhibits a bias towards adenine and cytosine,with an AT-skew of 0.075 and GC-skew of 0.291,respectively,and an A+T content of 84.3%.All PCGs are under purifying selection,and cox1 shows the slowest evolutionary rate.The repeat region located between trnE and trnF consists of 3.84 copies of 378-bp repeat units,and this region may be a common feature of the mitogenomes of Chaitophorinae.The phylogenetic results inferred from mitogenome sequences show that L.pruinosa is nested within the clade of Chaitophorus.Combined with the similarity between L.pruinosa and the species of Chaitophorus in both biological and morphological characteristics,this suggests that the validity of Lambersaphis is questionable.Our study provides valuable mitogenome data for further investigation into the phylogenetic relationships of Chaitophorinae and the systematic position of Lambersaphis. 展开更多
关键词 aphid mitogenome repeat region phylogeny
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Methyl salicylate reduces aphid abundance in maize through multiple modes of action 认领 引用
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作者 Yingyue Xu Xuanchen Zhou +4 位作者 Bin Yan Yang Yue Min Zhang Haibin Yuan Shuai Liu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2025年第10期3966-3977,共12页
Maize is a cornerstone of global food security,but it faces increasing challenges from corn aphids,particularly with the widespread adoption of genetically modified Bt maize.This trend suggests a growing need for sust... Maize is a cornerstone of global food security,but it faces increasing challenges from corn aphids,particularly with the widespread adoption of genetically modified Bt maize.This trend suggests a growing need for sustainable pest control strategies.Methyl salicylate has been proposed as a volatile compound with the potential for managing aphids.In this study,Y-tube olfactometer and Petri dish dispersal assays showed that methyl salicylate can repel wingless and winged aphids at 0.1 to 1,000 ngμL-1.Moreover,at concentrations of 100 and 1,000 ngμL-1,it was found to attract beneficial insects such as adults and larvae of Harmonia axyridis.Exposing maize plants to methyl salicylate resulted in a prominent reduction in the number of aphids compared to the control.In addition,clip cage experiment assays showed that the nymphal development duration was increased,while the adult duration and generation time were reduced,and the reproductive duration and total number of aphid offspring in plants treated with methyl salicylate were dramatically lower than in the control.Over two years of field trials,methyl salicylate-impregnated alginate beads provided significant reductions in the populations of key aphid species,including Rhopalosiphum padi,Rhopalosiphum maidis,and Aphis gossypii.Concurrently,there were marked increases in the presence of natural predators such as H.axyridis,Propylaea japonica,Syrphus corollae,and Chrysoperla sinica.These compelling results underscore the potential of methyl salicylate as a key component in integrated pest management strategies for maize,offering a green alternative to traditional chemical control. 展开更多
关键词 methyl salicylate aphids natural predators pest control
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Including predator presence in a refined model for assessing resistance of alfalfa cultivar to aphids 认领 引用 被引量:7
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作者 TU Xiong-bing FAN Yao-li +1 位作者 Mark McNeill ZHANG Ze-hua 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2018年第2期397-405,共9页
The aphid quantity ratio(AQR)is defined as the number of aphids on each cultivar divided by the number of aphids on all cultivars.AQR is based on the correlation between aphid populations and their host plants and is ... The aphid quantity ratio(AQR)is defined as the number of aphids on each cultivar divided by the number of aphids on all cultivars.AQR is based on the correlation between aphid populations and their host plants and is an important tool that has been utilized in evaluating Medicago sativa(alfalfa)cultivar resistance to aphids.However,assessment of alfalfa resistance to aphids can be confused by the presence of aphid predators,causing the assessment of plant resistance to aphids to be based on incorrect aphid population data.To refine the AQR and account for the effect of predators on aphid population assessments,we introduced a parameter‘α',corresponding to the predator quantity ratio,and usedαAQR as the ratio to quantify aphid populations.Populations of both aphids(4 species)and their predators(12 species)occurring in 28 M.sativa cultivars were sampled over two years at a research station near Cangzhou,Hebei Province,China.Results showed that the most suitable evaluation period was from May to June,as the aphid population was stable during this period.Compared with the AQR method,the predator population numbers based on theαAQR had a significant inverse relationship with aphid population numbers and the 28 cultivars were clustered into three classes:the resistant class,tolerant class,and susceptible class.In addition,17 cultivars were reassigned when evaluated usingαAQR.All numerical values calculated byαAQR were displayed as a Gaussian distribution,which showed that the 28 cultivars could be clustered into nine groups using a median value(±SE)of 1±0.1.Hence,ongoing alfalfa breeding trials will be assessed using theαAQR to establish a robust system that includes agronomic performance parameters in order to generalize the new method for further studies. 展开更多
关键词 aphid quantity ratio(AQR)model alfalfa cultivar resistance to aphids natural enemy
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Research on the Prevention and Control Technology of Rape Aphids 认领 引用 被引量:6
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作者 符明联 侯树敏 +6 位作者 朱炫 彭识 刘珏 杨和团 雷元宽 文和明 李根泽 《Agricultural Science & Technology》 CAS 2010年第11期141-144,共4页
[Objective] The aim was to screen economic and environment-friendly pesticides adaptive to aphid-resistant varieties in main rape growing regions of Yunnan Province.[Method] The effects of 6 pesticides against aphids ... [Objective] The aim was to screen economic and environment-friendly pesticides adaptive to aphid-resistant varieties in main rape growing regions of Yunnan Province.[Method] The effects of 6 pesticides against aphids on 22 Brassica campestris materials of the main breeds in Yunnan Province,the new improved varieties in China and the core breeding materials were tested.[Result] Yunyoushuang 1,Huayou 4,Yunhuayou Early-maturing Variety No.1 and A35 showed better aphid-resistance.An optimum pesticide application strategy was to use Diyaling in sowing time,and interchangeably apply nitenpyram and imidacloprid during flowering and pod formation stages.[Conclusion] The present study had provided an important technical support for sustainable development of rape industry. 展开更多
关键词 Rape Aphids Aphids-resistant rape Pesticides Prevention and control technology
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Resistance in Barley (Hordeum vulgareL.) to New Invasive Aphid, Hedgehog Grain Aphid (Sipha maydis, Passerini) (Hemiptera: Aphididae) 认领 引用
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作者 D. W. Mornhinweg G. J. Puterka J. S. Armstrong 《American Journal of Plant Sciences》 CAS 2020年第6期869-879,共11页
Sipha maydis Passerini (Hemiptera: Aphididae) is a pest of cereals in many regions of the world and was identified as an invasive pest of the US in 2007. Regional surveys from 2015-2017 revealed this pest was broadly ... Sipha maydis Passerini (Hemiptera: Aphididae) is a pest of cereals in many regions of the world and was identified as an invasive pest of the US in 2007. Regional surveys from 2015-2017 revealed this pest was broadly distributed throughout many of the western Great Plains states where it is a potential threat to cereal production. The common name hedgehog grain aphid, HGA, has been associated with Sipha maydis in the US. Cross-resistance where a plant is resistant to one aphid species and is also resistant to another species that is known to occur. Six barleys were evaluated for cross-resistance to HGA: Russian wheat aphid, RWA, resistant germplasms STARS 9301B and STARS 9577B and cultivar “Mesa”;greenbug, GB, resistant germplasm STARS 1501B and cultivar “Post 90”;and RWA and GB resistant experimental line 00BX 11-115. Cultivars “Morex” and “Schuyler” were susceptible controls. Antixenosis was measured 5 days after infestation by HGA. Seedling damage ratings and reductions in seedling growth were recorded after 17 days of infestation. Intrinsic rate of increase, rm, of HGA was determined by following the development of newborn aphids to adulthood and reproduction. 00BX 11-115 and Post 90 had significantly greater antixenosis (fewer aphids/seedling), significantly lower plant damage ratings, and significantly lower intrinsic rates of increase than other entries. Differences in seedling growth were not significant. 00BX 11-115 and Post 90 were the only entries with the Rsg1 greenbug resistance gene. Rsg1 greenbug resistance confers cross-resistance to HGA in the seedling stage. 展开更多
关键词 Greenbug Resistance Russian Wheat Aphid Resistance Aphid Resistance Antixenosis Plant Damage Ratings Hedgehog Grain Aphid
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Isolation and Identification of Pandora neoaphidis and Its Pathogenicity against Turnip Aphid(Lipaphis erysimi) 认领 引用
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作者 贾春生 《Plant Diseases and Pests》 2010年第6期43-45,共3页
[Objective] The study was to isolate and identify the pathogen of Pandora neoaphidis and to determine its pathogenicity against turnip aphid (Lipaphis erysimi).[Method] Based on the morphological characteristics,the... [Objective] The study was to isolate and identify the pathogen of Pandora neoaphidis and to determine its pathogenicity against turnip aphid (Lipaphis erysimi).[Method] Based on the morphological characteristics,the species of pathogenic fungus was identified.Spore shower method was used to carry out bioassay on the pathogen against turnip aphid.[Result] Primary conidia were ovoid,bitunicate and uninucleate,(24.7±1.4)μm×(10.7±0.9)μm,L/D=2.3±0.2.Secondary conidia had the similar shape with the primary ones,(18.6±2.1)μm×(13.3±1.3)μm,L/D=1.4±0.2.Hyphal body was like mycelium with the diameter of (10.6±0.8)μm.Conidiophores had palmate branch with the diameter of (10.0±0.9)μm.Pseudocystidia was not branched,which had rough base with the diameter of (19.2±1.7)μm,and gradually became more angular towards the apex with the diameter of (8.0±0.9)μm at tips.Rhizoid was like monohyphal shape with the diameter of (21.0±3.0)μm at base,the terminal apex had regular discoid holdfast.No resting spores were observed.The lethal dose of the pathogen against turnip aphid was 18.2/mm2.[Conclusion] The entomopathogenic fungus against turnip aphid was identified to be Pandora neoaphidis,and the pathogen was confirmed to have strong pathogenicity against turnip aphid. 展开更多
关键词 Pandora neoaphidis Pathogenicity Turnip aphid Entomopathogenic fungus Entomophthorales
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The biotypes and host shifts of cotton-melon aphids Aphis gossypii in northern China 认领 引用 被引量:7
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作者 ZHANG Shuai LUO Jun-yu +5 位作者 WANG Li WANG Chun-yi LU Li-min ZHANG Li-juan ZHU Xiang-zhen CUI Jin-jie 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2018年第9期2066-2073,共8页
Aphis gossypfi is a globally distributed species and therefore has a highly variable life cycle. Populations of A. gossypii in northern China exhibit greater genotypic diversity and a broader host range, yet the detai... Aphis gossypfi is a globally distributed species and therefore has a highly variable life cycle. Populations of A. gossypii in northern China exhibit greater genotypic diversity and a broader host range, yet the details of life cycles of different biotypes is still unclear. In this study, the Cytb and 16S gene regions of A. gossypfi collected from 5 common summer hosts and 4 primary hosts were analyzed. A total of 57 haplotypes were obtained from 1 046 individual A. gossyp# sequences. The sequence included 44 variable sites, 27 of which were parsimony informative sites and 17 of which were singleton variable sites. The most frequent 3 haplotypes were found in 896 individuals, representing a total of 85.7% of all individuals and 36 haplotypes were found in 1 individual. A neighbor-joining tree was constructed using 21 haplotypes that were found in more than 2 individuals. Considering the individual host plant, 5 biotypes were identified. Type 1 corresponded exactly to the cucurbit host-race and the other 4 biotypes were found as cotton host-races. Type 3 was the most abundant biotype in cotton fields in northern China. 展开更多
关键词 cotton-melon aphid Cytb and 16S genes haplotype biotype cotton field
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Physiology and defense responses of wheat to the infestation of different cereal aphids 认领 引用 被引量:7
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作者 LIU Fang-hua KANG Zhi-wei +3 位作者 TAN Xiao-ling FAN Yong-liang TIAN Hong-gang LIU Tongxian 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2020年第6期1464-1474,共11页
Cereal aphids are major insect pests of wheat,which cause significant damages to wheat production.Previous studies mainly focused on the resista nee of differe nt wheat varieties to one specific aphid species.However,... Cereal aphids are major insect pests of wheat,which cause significant damages to wheat production.Previous studies mainly focused on the resista nee of differe nt wheat varieties to one specific aphid species.However,reports on the physiology and defense responses of wheat to different cereal aphids are basically lacking.In this work,we studied the feeding behavior of three cereal aphids:the grain aphid,Sitobion avenae(Fabricius),the greenbugs,Schizaphis graminum(Ron dani),and the bird cherry-oat aphid,Rhopalosiphum padi(Linn aeus)on win ter wheat,and the physiology and defense responses of wheat to the infestation of these cereal aphids with focus on how these cereal aphids utilize divergent strategies to optimize their nutrition requirement from wheat leaves.Our results indicated that S.graminum and R.padi were better adapted to penetrating phloem tissue and to collect more nutrition than S.avenae.The harm on wheat physiology committed by S.graminum and R.padi was severer than that by S.avenae,through reducing chlorophyll concentration and interfering metabolism genes.Furthermore,cereal aphids manipulated the plant nutrition metabolism by increasing the relative concentration of major amino acids and percentage of essential amino acids.In addition,different cereal aphids triggered specific defense response in wheat.All of these results suggested that different cereal aphids utilize diverge nt strategies to cha nge the physiological and defe nse resp on ses of their host plants in order to optimize their nutriti on absorption and requireme nt.These fin dings not only exte nd our current knowledge on the insect-pla nt in teractions but also provide useful clues to develop no vel biotech no logical strategies for enhancing the resistance and toleranee of crop plants against phloem-feeding insects. 展开更多
关键词 cereal aphids feeding behavior nutrition physiology defense response
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Inter-species mRNA transfer among green peach aphids,dodder parasites,and cucumber host plants 认领 引用 被引量:8
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作者 Juan Song Jinge Bian +2 位作者 Na Xue Yuxing Xu Jianqiang Wu 《Plant Diversity》 SCIE CAS CSCD 2022年第1期1-10,共10页
mRNAs are transported within a plant through phloem.Aphids are phloem feeders and dodders(Cuscuta spp.)are parasites which establish phloem connections with host plants.When aphids feed on dodders,whether there is tra... mRNAs are transported within a plant through phloem.Aphids are phloem feeders and dodders(Cuscuta spp.)are parasites which establish phloem connections with host plants.When aphids feed on dodders,whether there is trafficking of mRNAs among aphids,dodders,and host plants and if aphid feeding a匚fects the mRNA transfer between dodders and hosts are unclear.We constructed a green peach aphid(GPA,Myzus persicae)-dodder(Cuscuta austra/is)-cucumber(Cucumis sativus)tritrophic system by infesting GPAs on C.australis,which parasitized cucumber hosts.We found that GPA feeding activated defense-related phytohormonal and transcriptomic responses in both C.australis and cucumbers and large numbers of mRNAs were found to be transferred between C.australis and cucumbers and between C.australis and GPAs;importantly,GPA feeding on C.australis greatly altered inter-species mobile mRNA profiles.Furthermore,three cucumber mRNAs and three GPA mRNAs could be respectively detected in GPAs and cucumbers.Moreover,our statistical analysis indicated that mRNAs with high abundances and long transcript lengths are likely to be mobile.This study reveals the existence of inter-species and even inter-kingdom mRNA movement among insects,parasitic plants,and parasite hosts,and suggests complex regulation of mRNA trafficking. 展开更多
关键词 Dodder Cuscuta Green peach aphid Host plant mRNA transfer Intei^kingdom interaction
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Insights into wing dimorphism in worldwide agricultural pest and hostalternating aphid Aphis gossypii 认领 引用 被引量:7
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作者 JI Jichao HUANGFU Ningbo +4 位作者 LUO Junyu GAO Xueke NIU Lin ZHANG Shuai CUI Jinjie 《Journal of Cotton Research》 CAS 2021年第1期38-49,共12页
Background:The worldwide pest Aphis gossypii has three-winged morphs in its life cycle,namely,winged parthenogenetic female(WPF),winged gynopara(GP),and winged male,which are all produced by a wingless parthenogenetic... Background:The worldwide pest Aphis gossypii has three-winged morphs in its life cycle,namely,winged parthenogenetic female(WPF),winged gynopara(GP),and winged male,which are all produced by a wingless parthenogenetic female(WLPF).Most studies on A.gossypii have focused on WPF,while few have investigated GP and male.The shared molecular mechanism underlying the wing differentiation in the three wing morphs of A.gossypii remains unknown.The wing differentiation of WPF was explored in a previous study.Herein,GP and male were induced indoors.The characters of the body,internal genitals,wing veins,and fecundity of GP and male were compared with those of WPF or WLPF.Compared with WLPF,the shared and separate differentially expressed genes(DEGs)were identified in these three-wing morphs.Results:Newly-born nymphs reared in short photoperiod condition(8 L:16D,18°C)exclusively produced gynoparae(GPe)and males in adulthood successively,in which the sex ratio was GP biased.A total of 14 GPe and 9 males were produced by one mother aphid.Compared with WLPF,the three-wing morphs exhibited similar morphology and wing vein patterns but were obviously discriminated in the length of fore-and underwings,reproductive system,and fecundity.A total of 37090 annotated unigenes were obtained from libraries constructed using the four morphs via RNA sequencing(RNA-Seq).In addition,10867 and 19334 DEGs were identified in the pairwise comparison of GP versus WLPF and male versus WLPF,respectively.Compared with WLPF,the winged morphs demonstrated 2335 shared DEGs(1658 upregulated and 677 downregulated).The 1658 shared upregulated DEGs were enriched in multiple signaling pathways,including insulin,FoxO,MAPK,starch and sucrose metabolism,fatty acid biosynthesis,and degradation,suggesting their key roles in the regulation of wing plasticity in the cotton aphid.Forty-four genes that spanned the range of differential expression were chosen to validate statistical analysis based on RNA-Seq through the reverse transcription quantitative real time polymerase chain reaction(RT-qPCR).The comparison concurred with the expression pattern(either up-or downregulated)and supported the accuracy and reliability of RNA-Seq.Finally,the potential roles of DEGs related to the insulin signaling pathway in wing dimorphism were discussed in the cotton aphid.Conclusions:The present study established an efficiently standardized protocol for GP and male induction in cotton aphid by transferring newly-born nymphs to short photoperiod conditions(8 L:16D,18°C).The external morphological characters,especially wing vein patterns,were similar among WPFs,GPe,and males.However,their reproductive organs were strikingly different.Compared with WLPF,shared(2335)and exclusively(1470 in WLPF,2419 in GP,10774 male)expressed genes were identified in the three-wing morphs through RNA-Seq,and several signaling pathways that are potentially involved in their wing differentiation were obtained,including insulin signaling,starch and sucrose metabolism. 展开更多
关键词 Cotton aphid Wing differentiation Wing plasticity Winged parthenogenetic female Gynopara Male
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Aphids and their transmitted potato viruses: A continuous challenges in potato crops 认领 引用 被引量:8
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作者 XU Yi Stewart M.GRAY 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2020年第2期367-375,共9页
Aphid is one of the most destructive insect pests on cultivated plants in temperate regions.Their piercing-sucking mouthparts and phloem feeding behavior directly damage crops and deplete plant nutrients.Potato(Solanu... Aphid is one of the most destructive insect pests on cultivated plants in temperate regions.Their piercing-sucking mouthparts and phloem feeding behavior directly damage crops and deplete plant nutrients.Potato(Solanum tuberosum L.)is one of the most important food sources on the planet,and several aphid species,e.g.,Myzus persicae(Sulzer)(green peach aphid)and Macrosiphum euphorbiae(Thomas)(potato aphid)(Hemiptera:Aphididae)colonize potato and transmit several economically important viruses.Aphid-transmitted potato viruses have been emerging all over the world as a very serious problem in potato production,inducing a wide variety of foliar and tuber symptoms,leading to severe yield reduction and loss of tuber quality.In this review,recent advances in understanding the interactions of potato viruses with their hosts,aphid vectors and the environment are described. 展开更多
关键词 aphids virus potato vector transmission
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Regional selection of insecticides and fungal biopesticides to control aphids and thrips and improve the forage quality of alfalfa crops 认领 引用 被引量:3
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作者 PAN Fan GAO Li-jie +6 位作者 ZHU Kai-hui DU Gui-lin ZHU Meng-meng ZHAO Li GAO Yu-lin TU Xiong-bing ZHANG Ze-hua 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2023年第1期185-194,共10页
The efficacies of biological and conventional chemical insecticides against two major insect pests of alfalfa(aphids and thrips)were compared in three sites across China’s alfalfa belt.In addition,the persistence of ... The efficacies of biological and conventional chemical insecticides against two major insect pests of alfalfa(aphids and thrips)were compared in three sites across China’s alfalfa belt.In addition,the persistence of the residues of chemical insecticides in alfalfa and their influence on the quality of alfalfa hay were examined.Efficacy varied among the different biological and chemical insecticides.The chemical insecticides were significantly more effective than biopesticides in a short time-frame.The efficacy period of biopesticides was significantly longer than that of chemical insecticides,and the corrected mortality rate of aphids in all regions was above 50%at 14 days after application.The analysis of pesticide residues showed that the residual doses of all the pesticides were within the allowed ranges after the safe period.The acid detergent fiber and neutral detergent fiber contents in alfalfa hay were higher and the protein content was lower in chemical insecticide treatments than in biopesticide treatments in Hebei.The relative feeding value of alfalfa hay treated with Metarhizium anisopliae IPP330189 was the highest among the treatments.Compared with chemical insecticides,the yield of alfalfa hay was higher in the biopesticides treatments.Biopesticides show a stronger control effect on insect populations and also a better improvement in the quality of alfalfa hay than chemical insecticides.This study provides a basis for exploring and developing a comprehensive control regime for alfalfa insect pests in the different alfalfa-growing regions in China,and for reducing chemical insecticide usage and improving forage quality. 展开更多
关键词 insect pest control pollution free control pesticide residue aphids thrips
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Identification of candidate genes for aphid resistance in upland cotton by QTL mapping and expression analysis 认领 引用 被引量:4
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作者 Qiushuang An Zhenyuan Pan +4 位作者 Nurimanguli Aini Peng Han Yuanlong Wu Chunyuan You Xinhui Nie 《The Crop Journal》 SCIE CSCD 2023年第5期1600-1604,共5页
Lignin is one of the main components of cell walls and is essential for resistance to insect pests in plants.Cotton plants are damaged by aphid(Aphis gossypii) worldwide but resistant breeding is undeveloped due to sc... Lignin is one of the main components of cell walls and is essential for resistance to insect pests in plants.Cotton plants are damaged by aphid(Aphis gossypii) worldwide but resistant breeding is undeveloped due to scarce knowledge on resistance genes and the mechanism. This study reported a lignin biosynthesisrelated gene identified in the F2 population derived from the cross between cotton cultivars Xinluzao 61(resistant to aphid) and Xinluzao 50(susceptible to aphid). A quantitative trait locus was mapped on chromosome D04 with a logarithm of odds(LOD) score of 5.99 and phenotypic effect of 27%. RNA-seq analysis of candidate intervals showed that the expression level of GH_D04G1418 was higher in the resistant cultivar than in the susceptible cultivar. This locus is close to AtLAC4 in the phylogenetic tree and contains a conserved laccase domain. Hence, it was designated GhLAC4-3. Silencing of GhLAC4-3 in Xinluzao 61 via virus-induced gene silencing(VIGS) resulted in decreased lignin content and increased susceptibility to aphids. These results suggest that GhLAC4-3 might enhance aphid resistance by regulating lignin biosynthesis in cotton. 展开更多
关键词 GhLAC4-3 Lignin Gossypium hirsutum Aphid resistance
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Transcriptome approach to understand the potential mechanisms of resistant and susceptible alfalfa(Medicago sativa L.) cultivars in response to aphid feeding 认领 引用 被引量:3
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作者 TU Xiong-bing ZHAO Hai-long ZHANG Ze-hua 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2018年第11期2518-2527,共10页
Plant breeding for resistance to agricultural pests is an essential element in the development of integrated crop management systems, however, the molecular and genetic mechanisms underlying resistance are poorly unde... Plant breeding for resistance to agricultural pests is an essential element in the development of integrated crop management systems, however, the molecular and genetic mechanisms underlying resistance are poorly understood. In this pilot study, we conducted a transcriptomic analysis of a resistant (R) and susceptible (S) variety of alfalfa, with (+A) or without (-A) aphids (totally four treatments). We used the resistant cultivar Zhongmu t and the susceptible cultivar Soca. A total of 3 549 mRNAs were differentially expressed, of which 1 738 up-regulated and 1 307 down-regulated genes were identified in S+A/S-A plants, while 543 up-regulated and 331 down-regulated genes were identified in the R+A/R-A plants. KEGG analysis mapped 112 and 546 differentially expressed genes to 8 and 17 substantially enriched pathways for Zhongmu 1 and Soca, respectively. Six shared pathways were linked to plant resistance traits, including phenylpropanoid biosynthesis associated with salicylic acid synthesis, and linoleic acid metabolism associated with both jasmonic acid and flavonoid biosynthesis. Ultimately, we proposed a preliminary regulatory mechanism of alfalfa cultivar resistance response to aphids feeding based on transcriptome analyses and published documents. 展开更多
关键词 alfalfa cultivar resistance aphids transcriptomic molecular mechanism
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Identification of Molecular Markers for a Aphid Resistance Gene in Sorghum and Selective Efficiency Using These Markers 认领 引用 被引量:4
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作者 CHANG Jin-hua CUI Jiang-hui +1 位作者 XUE Wei ZHANG Qing-wen 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2012年第7期1086-1092,共7页
In this study, an F2 segregated population obtained by hybridization between the aphid-sensitive sorghum strain Qiansan and aphid-resistant cultivar Henong 16 was used to establish an aphid-resistant pool and an aphid... In this study, an F2 segregated population obtained by hybridization between the aphid-sensitive sorghum strain Qiansan and aphid-resistant cultivar Henong 16 was used to establish an aphid-resistant pool and an aphid-sensitive pool. 192 pairs of AFLP (amplified fragment length polymorphism) marker primers were screened in these pools using BSA (bulked segregant analysis). Three pairs of EcoR I-CTG/Mse I-CCT, EcoR I-CTG/Mse I-CAT, and EcoR I-AGT/Mse I-CCC showed linkage with aphis resistance. EcoR I-CTG/Mse I-CCT-475, EcoR I-CTG/Mse I-CAT-390, and EcoR I-AGT/Mse I-CCC- 350 (E42/M52-350) were mapped within 6, 10, and 13 cM distances with the aphid-resistant gene by using Mapmaker 3.0 software. The bands amplified by EcoR I-CTG/Mse I-CCT-475 and EcoR I-CTG/Mse I-CAT-390 were extracted, cloned, and sequenced. Specific primers of SCAR (sequence characterized amplified regions) were then designed from these bands. A specific band of 300 bp was amplified by a pair of SCAR primers designed based on the sequence obtained from the EcoR I-CTG/Mse I-CAT-390 marker. The SCAR marker was named SCAS0. The marker was used to detect the F2, BC1, and F2:3 populations. The selective efficiency was 86.8, 91.1, and 86.3% in the BC1, F2, and F2:3 populations, respectively. The average selective efficiency was 88.2%. 展开更多
关键词 sorghum bicolor aphid resistance gene molecular marker SCAR molecular assistant selection
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Reproductive polyphenism and its advantages in aphids:Switching between sexual and asexual reproduction 认领 引用 被引量:4
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作者 YAN Shuo WANG Wan-xing SHEN Jie 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2020年第6期1447-1457,共11页
Reproductive polyphenism,which allows one genotype to produce sexual and asexual morphs,is an extreme case of phenotypic plasticity and is commonly observed in aphids.Aphids are typical species that switch these repro... Reproductive polyphenism,which allows one genotype to produce sexual and asexual morphs,is an extreme case of phenotypic plasticity and is commonly observed in aphids.Aphids are typical species that switch these reproductive modes,and the pathway orientation is triggered by the environmental conditions(mainly photoperiod and temperature).The typical arm ual life of aphids in eludes a successi on of parthenogenetic gen erations duri ng the spri ng and summer and a single sexual generation in autumn.In this review,we describe how the environmental cues orientate the reproductive mode of aphids from photoperiodic perception to endocrine regulation,and how juvenile hormones may act on the target cells(oocytes)to initiate the gametogenesis and embryogenesis in sexual and asexual reproduction.We also discuss the paradox of sex,especially the advantages of sexual reproduction in aphids.With the recent development of genomic resources in aphids,many potential genes involved in the reproductive polyphenism will enter the public's awareness.In particular,we describe a novel RNAi method in aphids,which may provide a molecular technique for determining the developmental fate and multiple reproductive strategies. 展开更多
关键词 aphids parthenogenesis embryogenesis gametogenesis photoperiodism
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Transgenic Tobacco Expressing Lycoris radiata Agglutinin Showed Enhanced Resistance to Aphids 认领 引用 被引量:9
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作者 PANG Yong-Zhen YAO Jian-Hong +4 位作者 SHEN Guo-An QI Hua-Xiong TAN Feng SUN Xiao-Fen TANG Ke-Xuan 《Acta Botanica Sinica》 2004年第7期767-772,共6页
Transgenic plants expressing foreign genes resulting in resistance against pests could make significant contribu-tion to sustainable agriculture.Considerable progress has been achieved to control the chewing insect pe... Transgenic plants expressing foreign genes resulting in resistance against pests could make significant contribu-tion to sustainable agriculture.Considerable progress has been achieved to control the chewing insect pests by the development of transgenic plants expressing Bacillus thuringiensis endotoxins(Barton and Miller,1993)or plant-derived proteins such as protease inhibitor(Hoffman et al.,1992;Hilder et al.,1993)or lectins(Boulter et al.,1990).However,there are fewer reports on the successful control of sap-sucking insects belonging to the order Homoptera by the use of genetic engineering technology. 展开更多
关键词 insect bioassay Lycoris radiata agglutinin(LRA) transgenic tobacco peach potato aphid(Myzus persicae)
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Enhancing aphid resistance in horticultural crops:a breeding prospective 认领 引用 被引量:1
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作者 Lili Zhang Chaoyan Chen +9 位作者 Yao Li Chunyu Suo Wei Zhou Xiaowei Liu Yizhuo Deng Hamza Sohail Ziyi Li Fang Liu Xuehao Chen Xiaodong Yang 《Horticulture Research》 SCIE CSCD 2024年第12期132-143,共12页
Increasing agricultural losses caused by insect infestations are a significant problem,so it is important to generate pest-resistant crop varieties to address this issue.Several reviews have examined aphid-plant inter... Increasing agricultural losses caused by insect infestations are a significant problem,so it is important to generate pest-resistant crop varieties to address this issue.Several reviews have examined aphid-plant interactions from an entomological perspective.However,few have specifically focused on plant resistance mechanisms to aphids and their applications in breeding for aphid resistance.In this review,we first outline the types of resistance to aphids in plants,namely antixenosis,tolerance(cell wall lignification,resistance proteins),and antibiosis,and we discuss strategies based on each of these resistance mechanisms to generate plant varieties with improved resistance.We then outline research on the complex interactions amongst plants,viruses,and aphids,and discuss how aspects of these interactions can be exploited to improve aphid resistance.A deeper understanding of the epigenetic mechanisms related to induced resistance,i.e.the phenomenon where plants become more resistant to a stress they have encountered previously,may allow for its exploitation in breeding for aphid resistance.Wild relatives of crop plants serve as important sources of resistance traits.Genes related to these traits can be introduced into cultivated crop varieties by breeding or genetic modification,and de novo domestication of wild varieties can be used to exploit multiple excellent characteristics,including aphid resistance.Finally,we discuss the use of molecular design breeding,genomic data,and gene editing to generate new aphid-resistant,high-quality crop varieties. 展开更多
关键词 plant resistance mechanisms insect infestations wild relatives genetic modification induced resistance aphid resistance breeding strategies epigenetic mechanisms
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Transgenic Expression of a Functional Fragment of Harpin Protein Hpa1 in Wheat Represses English Grain Aphid Infestation 认领 引用 被引量:1
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作者 XU Man-yu ZHOU Ting +4 位作者 ZHAO Yan-ying LI Jia-bao XU Heng DONG Han-song ZHANG Chun-ling 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2014年第12期2565-2576,共12页
The harpin protein Hpa1 produced by the rice bacterial blight pathogen promotes plant growth and induces plant resistance to pathogens and insect pests. The region of 10-42 residues (Hpa110-42) in the Hpa1 sequence ... The harpin protein Hpa1 produced by the rice bacterial blight pathogen promotes plant growth and induces plant resistance to pathogens and insect pests. The region of 10-42 residues (Hpa110-42) in the Hpa1 sequence is critical as the isolated Hpa110-42 fragment is 1.3-7.5-fold more effective than the full length in inducing plant growth and resistance. Here we report that transgenic expression of Hpa110-42 in wheat induces resistance to English grain aphid, a dominant species of wheat aphids. Hpa110-42-induced resistance is effective to inhibit the aphid behavior in plant preference at the initial colonization stage and repress aphid performances in the reproduction, nymph growth, and instar development on transgenic plants. The resistance characters are correlated with enhanced expression of defense-regulatory genes (EIN2, PP2-A, and GSL10) and consistent with induced expression of defense response genes (Hel, PDF1.2, PR-1b, and PR-2b). As a result, aphid infestations are alleviated in transgenic plants. The level of Hpa110-42-induced resistance in regard to repression of aphid infestations is equivalent to the effect of chemical control provided by an insecticide. These results suggested that the defensive role of Hpa110-42 can be integrated into breeding germplasm of the agriculturally signiifcant crop with a great potential of the agricultural application. 展开更多
关键词 Hpa110-42 transgenic wheat resistance aphids chemical control
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