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Astaxanthin ameliorates cardiac damage induced by prepubertal di(2-ethylhexyl)phthalate exposure via inhibition of PPAR-α-mediated excessive autophagy 认领 引用
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作者 Bowen Yin Xuanyi Liu +8 位作者 Yanyi Zhai Miaomiao Lu Xiaoya Sun Jingyi Ren Yadong Zhang Yiran Li Dinghui Zhao Mingyu Li Yuxia Ma 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2026年第8期785-798,共14页
Di(2-ethylhexyl)phthalate(DEHP),a widely used plasticizer,impairs cardiac development and increases the risk of cardiovascular disease.Astaxanthin(ASX),a natural carotenoid,has recognized cardiovascular benefits,but i... Di(2-ethylhexyl)phthalate(DEHP),a widely used plasticizer,impairs cardiac development and increases the risk of cardiovascular disease.Astaxanthin(ASX),a natural carotenoid,has recognized cardiovascular benefits,but its protective effects against DEHP-induced cardiac injury and underlying mechanisms remain unclear.Using transcriptomic data,bioinformatic analysis identified signaling pathways associated with DEHP-induced cardiac injury.Network pharmacology integrated with machine learning predicted potential ASX targets.The cardioprotective effects of ASX were validated in C57BL/6 mice and H9c2 cells to explore mechanisms in vivo and in vitro.Bioinformatic analysis revealed autophagy as a central pathway in DEHP cardiotoxicity,with PPAR-αidentified as a hub gene regulating autophagy.Experimental results showed that ASX significantly alleviated DEHP-induced cardiac injury,demonstrated by improved histopathology,reduced myocardial enzyme levels,and decreased oxidative stress.Western blotting confirmed that ASX reduced the expression of PPAR-α,Beclin 1,and LC3,whereas increasing p62 expression in DEHP-exposed mice and MEHP-treated H9c2 cells.These findings demonstrate that ASX mitigates DEHP-induced cardiac injury by suppressing autophagy through selective inhibition of PPAR-α.ASX supplementation may represent a promising dietary strategy to counteract cardiovascular toxicity from environmental pollutants. 展开更多
关键词 Astaxanthin Di(2-ethylhexyl)phthalate Cardiac injury Autophagy Network pharmacology
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Mitochondria-targeted nanoparticles for encapsulating astaxanthin:exploring the reason for alleviating oxidative damage 认领 引用 被引量：1
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作者 Yannan Chen Siyuan Fei +1 位作者 Lijuan Zhang Mingqian Tan 《Food Science and Human Wellness》 SCIE CAS CSCD 2025年第1期124-133,共10页
Oxidative stress is considered as a critical factor in the process of pathological diseases,and mitochondria are considered as vital target organelles for disease intervention.The purpose of this study was aimed to ev... Oxidative stress is considered as a critical factor in the process of pathological diseases,and mitochondria are considered as vital target organelles for disease intervention.The purpose of this study was aimed to evaluate the antioxidant efficacy of mitochondria-targeted astaxanthin nanoparticle on hydrogen peroxideinduced oxidative damage.As expected,mitochondria-targeted nanoparticle showed excellent mitochondria co-localization ability with higher Pearson's correlation coefficient(r=0.88).In vitro experiments suggested that the mitochondria-targeted astaxanthin nanoparticle could promote cell viability and increase antioxidantrelated enzyme activities.Simultaneously,metabolomics analysis indicated that mitochondria-targeted astaxanthin nanoparticle could alleviate oxidative stress by regulating amino acid metabolism and energy metabolism.Altogether,all these results strongly confirmed the mitochondria-targeted strategy for astaxanthin delivery could relieve oxidative stress and had great promise in the application of disease intervention. 展开更多
关键词 Oxidative stress Mitochondria-targeted Astaxanthin Antioxidant Metabolism
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1H NMR Quantification of Astaxanthin and Vitamin E in Nutritional Supplement 认领 引用
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作者 XIN Wenyuan ZHOU Yunbing +2 位作者 LIU Daixi WANG Cong SHERWIN K.B.Sy 《Journal of Ocean University of China》 SCIE CAS CSCD 2025年第6期1827-1833,共7页
Astaxanthin(AX)and vitamin E(VE)are widely consumed nutritional supplements in China,with its beneficial effects predominantly attributed to all-trans AX and VE.The aim of this study is to develop and validate a rapid... Astaxanthin(AX)and vitamin E(VE)are widely consumed nutritional supplements in China,with its beneficial effects predominantly attributed to all-trans AX and VE.The aim of this study is to develop and validate a rapid and accurate method for quantifying the content of AX and VE in nutritional supplement products using highly sensitive1H NMR method.Coumarin was chosen as the internal standard.Specific signals from AX was optimal at H-7,7'in the chemical shift range ofδ6.17–6.24 ppm,whereas the signals of VE atδ2.59 ppm.To demonstrate the reliability of this analytical approach the proposed method underwent rigorous validation,specificity,limit of detection(LOD),limit of quantitation(LOQ),linearity,accuracy,precision,and recovery.The accuracy of the validation method was 3.10%for AX and 1.99%for VE.The results indicated that the method was precise and reliable.The method has been successfully applied to simultaneous quantification of AX and VE in nutritional supplements products. 展开更多
关键词 1H NMR spectroscopy astaxanthin vitamin E quality control marine bioresource
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Astaxanthin ameliorates benzalkonium chloride-induced dry eye disease through suppressing inflammation and oxidative stress via Keap1- Nrf2/HO- 1 signaling pathways 认领 引用 被引量：5
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作者 Ziyu Liu Yaqiong Li +7 位作者 Jiayu Bao Siyuan Li Ya Wen Peng Zhang Jun Feng Yinghui Wang Lei Tian Ying Jie 《Animal Models and Experimental Medicine》 CAS CSCD 2025年第6期1056-1079,共24页
Background:Dry eye disease(DED)predominantly results from elevated tear film os-molarity,which can not only cause ocular inconvenience but may lead to visual impair-ments,severely compromising patient well-being and e... Background:Dry eye disease(DED)predominantly results from elevated tear film os-molarity,which can not only cause ocular inconvenience but may lead to visual impair-ments,severely compromising patient well-being and exerting substantial economic burdens as well.Astaxanthin(AST),a member of the xanthophylls and recognized for its robust abilities to combat inflammation and oxidation,is a common dietary sup-plement.Nonetheless,the precise molecular pathways through which AST influences DED are still poorly understood.Methods:Therapeutic targets for AST were identified using data from the GeneCards,PharmMapper,and Swiss Target Prediction databases,and STITCH datasets.Similarly,targets for dry eye disease(DED)were delineated leveraging resources such as the Therapeutic Target Database(TTD),DisGeNET,GeneCards,and OMIM databases,and DrugBank datasets.Interactions among shared targets were charted and dis-played using CytoScape 3.9.0.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were conducted to elucidate the functions of pivotal tar-gets within the protein-protein interaction network.Molecular interactions between AST and key targets were confirmed through molecular docking using AutoDock and PyMOL.Molecular dynamics simulations were performed using GROMACS 2022.3.Viability of human corneal epithelial cells(hCEC)was assessed across varying concen-trations of AST.A mouse model of experimental DED was developed using 0.1%ben-zalkonium chloride(BAC),and the animals were administered 100 mg/kg/day of AST orally for 7 days.The efficacy of the treatments was assessed through a series of di-agnostic tests to evaluate the condition of the ocular surface after the interventions.The levels of inflammation and oxidative stress were quantitatively assessed using methods such as reverse transcription-polymerase chain reaction(RT-PCR),Western blot,and immunofluorescence staining.Results:Network pharmacology suggests that AST may alleviate DED by influenc-ing oxidation-reduction signaling pathways and reducing oxidative stress provoked by BAC.In vivo experiments demonstrated an improved overall condition in AST-administered mice in contrast to the control group.Immunofluorescence staining analyses indicated a decrease in Keap1 protein in the corneal tissues of AST-treated mice and a significant increase in Nrf2 and HO-1 protein.In vitro studies demon-strated that AST significantly enhanced cell viability and suppressed reactive oxy-gen species expression under hyperosmotic(HS)conditions,thereby protecting the human corneal epithelium.Conclusion:AST is capable of shielding mice from BAC-induced DED,decelerating the progression of DED,and mitigating oxidative stress damage under HS conditions in hCEC cells.The protective impact of AST on DED may operate through stimulating the Keap1-Nrf2/HO-1 signaling pathway.Our research findings indicate that AST may be a promising treatment for DED,offering new insights into DED treatment. 展开更多
关键词 astaxanthin dry eye disease human corneal epithelial cell Keap1-Nrf2/HO-1 pathway,network pharmacology oxidative stress
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Supercritical carbon dioxide extraction of astaxanthin from Corynebacterium glutamicum 认领 引用 被引量：1
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作者 Jan Seeger Maximilian Zäh +2 位作者 Volker F.Wendisch Christoph Brandenbusch Nadja A.Henke 《Bioresources and Bioprocessing》 SCIE EI CSCD 2025年第1期741-749,共9页
Astaxanthin,a red carotenoid with potent antioxidant properties,holds significant value in the feed,cosmetics,and nutraceutical industries.While traditionally sourced from microalgae,Corynebacterium glutamicum,a well-... Astaxanthin,a red carotenoid with potent antioxidant properties,holds significant value in the feed,cosmetics,and nutraceutical industries.While traditionally sourced from microalgae,Corynebacterium glutamicum,a well-established industrial microorganism,has been engineered to serve as an efficient host for astaxanthin production.As astaxanthin integrates into the cellular membrane,effective extraction methods are essential to access this valuable compound.In this study,a sustainable batch extraction process using supercritical carbon dioxide(scCO2)as a green solvent was developed.The effects of cosolvent concentration(0-9%(w/w)),temperature(50-75℃),and pressure(450-650 bar)were investigated with regard to the extraction yield.An optimized extraction was achieved with 9%(w/w)ethanol as a cosolvent,at 68℃ and 550 bar,allowing the extraction of 67.5±3.7%of the cellular astaxanthin within 0.5 h.Prolonging the extraction time further increased the recovery to 93.3%,which is comparable to processes that have been established for the extraction of astaxanthin from microalgae and yeast.This approach provides a scalable and environmentally friendly solution for industrial astaxanthin recovery. 展开更多
关键词 Astaxanthin Supercritical carbon dioxide Extraction Corynebacterium glutamicum
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Docosahexaenoic acid-acylated astaxanthin monoester enhances microglial autophagy for ameliorating amyloid-βload and cognitive deficits in models of Alzheimer's disease 认领 引用
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作者 Xiaoxu Wang Bo Dong +4 位作者 Yu Song Zhigao Wang Yuming Wang Jie Xu Changhu Xue 《Food Science and Human Wellness》 SCIE CAS CSCD 2025年第6期2148-2160,共13页
Autophagy directly regulates the amyloid beta-peptide(Aβ)clearance,and its dysfunction occurs in the early pathogenesis of Alzheimer's disease(AD).We previously reported that docosahexaenoic acid-acylated astaxan... Autophagy directly regulates the amyloid beta-peptide(Aβ)clearance,and its dysfunction occurs in the early pathogenesis of Alzheimer's disease(AD).We previously reported that docosahexaenoic acid-acylated astaxanthin monoester(AST-DHA)showed neuroprotection against AD pathology.However,its in-depth mechanism and autophagic responses in AD brains are poorly understood.Herein,SH-SY5Y cells overexpressing the Swedish mutation(K595N/M596L)of APP gene were established to preliminarily evaluate the actions of AST-DHA on reducing Aβ1-42levels and regulating autophagy.In microglial BV2 cells,AST-DHA and free astaxanthin(F-AST)recovered p62 and LC3Ⅱ/Ⅰlevels,and restored autophagy flux by rescuing the late phase of microglial autophagy.Notably,autophagic inhibitor bafilomycin A1 blunted the abilities of AST-DHA to reduce Aβ1-42and fibral Aβ,suggesting that AST-DHA probably promoted Aβclearance in a microglial autophagy-dependent manner.Further studies in APP/PS1 mice verified that dietary AST-DHA and F-AST promoted Aβphagocytosis via microglial autophagy.Significant decreases of Iba1 and p62 were observed around Aβplaque in the hippocampus and cortex using triple fluorescence staining.Furthermore,AST-DHA exhibited superior performance over F-AST in restoring autophagic dysfunction,ameliorating Aβburden and cognitive deficit.Our findings suggest a possible mechanism of AST-DHA in improving AD by which it restores microglial autophagy to facilitate cerebral Aβclearance.It supports the future application of AST-DHA as an autophagic regulator in maintaining brain function. 展开更多
关键词 Autophagy Amyloidβ-peptide Astaxanthin Microglia Cognitive function
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Novel insights into saline stress on photosynthetic activity and astaxanthin production of Haematococcus pluvialis 认领 引用
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作者 Cuili JIN Jiajie YOU +2 位作者 Zepeng ZHOU Qing LIU Xiaojian ZHOU 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2025年第3期921-938,共18页
Saline treatment is a low-cost,simple,and effective method to stimulate astaxanthin accumulation in Haematococcus pluvialis,and is proposed to be applied in the second stage of a 2-stage culture since it does not nece... Saline treatment is a low-cost,simple,and effective method to stimulate astaxanthin accumulation in Haematococcus pluvialis,and is proposed to be applied in the second stage of a 2-stage culture since it does not necessitate changing the medium.To understand the effect of salinity on the astaxanthin production of H.pluvialis,the photosynthetic activity and the biocomponents production in 1-and 2-stage cultures in different salinities were investigated.Except for astaxanthin synthesis,which increased at low salinities of 2 and 5-g/L NaCl,most biocomponent yields decreased in 1-stage cultures as salinity increased.At a salinity of 5-g/L NaCl,the 2-stage culture further increased astaxanthin production to 18.41±0.24 mg/L,which was more than 2.0 times that of the control.Saline treatment led to an overall decrease in photosynthetic performance indices of H.pluvialis,and had an impact on five sites of the electron transport chain:the energy connection between antenna and reaction center of photosystemⅡ(PSⅡ),oxygen evolving complex activity on the donor side,the electron transfer from plastoquinone A(QA)to plastoquinone B(QB)and from plastoquinone(PQ)to receptor side of photosystem I(PS I),and the pool size of the end electron acceptors in PSⅠacceptor side.The excitation imbalance between PSⅠand PSⅡcaused by the variance in the electron transfer chain necessitated the synthesis of antioxidants like astaxanthin in order to ensure cell viability.The accumulation of astaxanthin was found to be closely correlated with the stabilized or enhanced the maximum relative electron transfer rate(rETRmax)and the PSⅡactual quantum yield(QYSS)as well as the increased fluorescence yield at J-step(VJ).This work offers the novel insight of how saline stress controls H.pluvialis photosynthetic activity and astaxanthin synthesis. 展开更多
关键词 Haematococcus pluvialis salinity 2-stage culture astaxanthin production photosynthetic activity
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Comparison of the Digestion and Absorption Characteristics of Docosahexaenoic Acid-Acylated Astaxanthin Monoester and Diester in Mice 认领 引用 被引量：3
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作者 LI Ye YU Jie +5 位作者 XU Jie CHE Hongxia WANG Yuming XUE Changhu HUANG Qingrong ZHANG Tiantian 《Journal of Ocean University of China》 SCIE CAS CSCD 2021年第4期973-984,共12页
Docosahexaenoic acid-acylated astaxanthin(DHA-AST)esters exhibit distinct bioactivities in improving brain function.However,the digestion and absorption characteristics of DHA-AST esters in vivo are unclear,thereby re... Docosahexaenoic acid-acylated astaxanthin(DHA-AST)esters exhibit distinct bioactivities in improving brain function.However,the digestion and absorption characteristics of DHA-AST esters in vivo are unclear,thereby restricting the molecular mechanism analysis of their superior activities.This study compared the digestion and absorption characteristics of DHA-AST monoester and diester by determining the levels of AST and DHA in the serum,liver,small intestinal content and wall,and feces at different time points after a single-dose oral administration of the esters.After oral gavage with 2 mg AST equivalent of the DHA-AST monoester and diester for 18 h,the excretion rates were approximately 51%and 84%,respectively.This result indicates that DHA-AST monoester was better than diester for the absorption of AST.The results in serum,liver,and small intestinal content and wall also agreed with this finding.Moreover,the excretion rates of DHA in the feces at 24 h in the DHA-AST monoester and diester groups were approximately 40%and 36%after gavage with 5 mg DHA equivalent,respectively.This result indicates that DHA-AST diester exhibited a better tendency than monoester for the absorption of DHA.Interestingly,the results in the liver and small intestinal wall showed an apparent difference,indicating that DHA-AST diester was better than monoester for the absorption of DHA.These findings provide a scientific basis for the molecular mechanism analysis and utilization of DHA-AST monoester and diester as functional ingredients. 展开更多
关键词 DHA-acylated astaxanthin monoester DHA-acylated astaxanthin diester digestion absorption
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Research Progress on Astaxanthin in Exercise- Induced Fatigue 认领 引用
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作者 Guanyinliang Wen 《Journal of Clinical and Nursing Research》 2025年第11期316-322,共7页
Exercise-induced fatigue represents a complex physiological response triggered by physical exertion,with its mechanisms primarily originating from central and peripheral systems.Central fatigue arises from neurotransm... Exercise-induced fatigue represents a complex physiological response triggered by physical exertion,with its mechanisms primarily originating from central and peripheral systems.Central fatigue arises from neurotransmitter imbalances such as elevated serotonin and reduced dopamine levels,leading to drowsiness and diminished motor performance.Peripheral fatigue occurs at the muscular level,where energy depletion,metabolic waste accumulation,and oxidative stress impair muscle contraction function.Astaxanthin,a potent antioxidant,directly and primarily alleviates peripheral fatigue through its antioxidant,anti-inflammatory,and mitochondrial protective effects.Simultaneously,by improving the peripheral environment and reducing the transmission of fatigue signals to the brain,it indirectly helps alleviate central fatigue.Based on this,this paper reviews the mechanisms of action and related research progress of astaxanthin on exercise-induced fatigue,and discusses its application value and challenges based on the current status. 展开更多
关键词 Exercise-induced fatigue Astaxanthin Mechanism of action Oxidative stress Anti-inflammatory
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Combined conventional/antioxidant“Astaxanthin”treatment for male infertility:a double blind,randomized trial 认领 引用 被引量：11
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作者 F.H.Comhaire Y.El Garem +2 位作者 A.Mahmoud F.Eertmans F.Schoonians 《Asian Journal of Andrology》 SCIE CAS 2005年第3期257-262,共6页
Aim： To evaluate the treatment of male infertility with a strong natural antioxidant, in addition to conventional treatment. Methods： Using a double blind, randomized trial design, 30 men with infertility of ≥12 mo... Aim： To evaluate the treatment of male infertility with a strong natural antioxidant, in addition to conventional treatment. Methods： Using a double blind, randomized trial design, 30 men with infertility of ≥12 months and female partners with no demonstrable cause of infertility received conventional treatment according to the guidelines of the World Health Organization （WHO）, and either a strong antioxidant Astaxanthin 16 rag/day （AstaCarox, AstaReal AB, Gustavsberg, Sweden） or placebo for 3 months. The effects of treatment on semen parameters, reactive oxygen species （ROS）, zona-free hamster oocyte test, serum hormones including testosterone, luteinizing hormone （LH）, follicle stimulating hormone （FSH） and Inhibin B, and spontaneous or intrauterine insemination （IUI）-induced pregnancies were evaluated. Results： ROS and Inhibin B decreased significantly and sperm linear velocity increased in the Astaxanthin group （n = 11）, but not in the placebo group （n = 19）. The results of the zona-free hamster oocyte test tended to improve in the Astaxanthin group in contrast with the placebo group, though not reaching statistical significance. The total and per cycle pregnancy rates among the placebo cases （10.5 % and 3.6 %） were lower compared with 54.5 % and 23. 1% respectively in the Astaxanthin group （P=0.028; P=0.036）. Conclusion： Although the present study suggests a positive effect of Astaxanthin on sperm parameters and fertility, the results need to be confirmed in a larger trial before recommending Astaxanthin for the complementary treatment of infertile men. （Asian J Androl 2005 Sep; 7： 257-262） 展开更多
关键词 male infertility antioxidant astaxanthin ROS treatment pregnancy
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Optimization of acidic extraction of astaxanthin from Phaffia rhodozyma 认领 引用 被引量：15
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作者 Hui NI Qi-he CHEN +2 位作者 Guo-qing HE Guang-bin WU Yuan-fan YANG 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS 2008年第1期51-59,共9页
Optimization of a process for extracting astaxanthin from Phaffia rhodozyma by acidic method was investigated, regarding several extraction factors such as acids, organic solvents, temperature and time. Fractional fac... Optimization of a process for extracting astaxanthin from Phaffia rhodozyma by acidic method was investigated, regarding several extraction factors such as acids, organic solvents, temperature and time. Fractional factorial design, central composite design and response surface methodology were used to derive a statistically optimal model, which corresponded to the following optimal condition： concentration of lactic acid at 5.55 mol/L, ratio of ethanol to yeast dry weight at 20.25 ml/g, temperature for cell-disruption at 30 ℃, and extraction time for 3 min. Under this condition, astaxanthin and the total carotenoids could be extracted in amounts of 1294.7 μg/g and 1516.0 μg/g, respectively. This acidic method has advantages such as high extraction efficiency, low chemical toxicity and no special requirement of instruments. Therefore, it might be a more feasible and practical method for industrial practice. 展开更多
关键词 Astaxanthin Phaffia rhodozyma Extracting Optimization Acidic method
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Effects of dietary supplementation with algal astaxanthin on growth,pigmentation,and antioxidant capacity of the blood parrot (Cichlasoma citrinellum × Cichlasoma synspilum ) 认领 引用 被引量：10
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作者 LI Feng HUANG Shuiying +5 位作者 LU Xiaoxia WANG Jun LIN Mingwei AN Yu WU Shaoting CAI Minggang 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2018年第5期1851-1859,共9页
An algal astaxanthin feeding trial was carried out to investigate the ef fects of natural astaxanthin from Haematococcus pluvialis as feed additives on growth, pigmenting efficacy and antioxidant capacity in blood par... An algal astaxanthin feeding trial was carried out to investigate the ef fects of natural astaxanthin from Haematococcus pluvialis as feed additives on growth, pigmenting efficacy and antioxidant capacity in blood parrot(C ichlasoma citrinellum × C ichlasoma. synspilum). Tissue total antioxidant capacity(TAC), superoxide dismutase(SOD), catalase(CAT) and maleic dialdehyde(MDA) were chosen as measures of its antioxidant capacity. All fish which received an astaxanthin(from micro-algal H. pluvialis) supplemented diet with 400 mg/kg of astaxanthin, after 50 days of feeding, the astaxanthin-fed fish displayed a pinkcolored skin and the control-fed fish displayed a grayish skin. For the growth, the weight gains of controlfed fish and astaxanthin-fed fish were 200% and 300%, respectively. Samples of skin and scales were used for analysis of total carotenoids and astaxanthin content, and fish feeding astaxanthin showed significantly( P <0.05) higher concentrations than the control group, indicating that the pigmentation of this fish had been significantly improved by dietary astaxanthin. Compared with the control fish, pigmented fish had lower SOD, CAT and MDA and higher TAC. It can be concluded that supplementation with dietary astaxanthin could eff ectively enhance growth, skin coloration and the antioxidant capacity of this fish. This study will provide a reference for application of natural astaxanthin from H. pluvialis as feed additives in blood parrot artificial breeding. Our data is also useful in ornamental fish farming, especially when the retentivity of astaxanthin in the skin and scales are involved. It is leading to the possibility of increasing the pigmentation of farmed-fish by adding the powdered form of H. pluvialis to the diet as an ef fective pigment. 展开更多
关键词 astaxanthin Haematococcus pluvialis pigmentation antioxidant capacity blood parrot
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Studies on optimization of nitrogen sources for astaxanthin production by Phaffia rhodozyma 认领 引用 被引量：11
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作者 NI Hui CHEN Qi-he +5 位作者 RUAN Hui YANG Yuan-fan LI Li-jun WU Guang-bin Hid Yang HE Guo-qing 《Journal of Zhejiang University-SCIENCE B》 EI CAS 2007年第5期365-370,共6页
Fermentation of Phaffia rhodozyma is a major method for producing astaxanthin, an important pigment with industrial and pharmaceutical application. To improve astaxanthin productivity, single factor and mixture design... Fermentation of Phaffia rhodozyma is a major method for producing astaxanthin, an important pigment with industrial and pharmaceutical application. To improve astaxanthin productivity, single factor and mixture design experiments were used to investigate the effects of nitrogen source on Phaffia rhodozyma cultivation and astaxanthin production. Results of single factor experiments showed nitrogen source could significantly affect P. rhodozyma cultivation with respect to carbon source utilization, yeast growth and astaxanthin accumulation. Further studies of mixture design experiments using (NH4)2SO4, KNO3 and beef extract as nitrogen sources indicated that the proportion of three nitrogen sources was very important to astaxanthin production. Validation experiments showed that the optimal nitrogen source was composed of 0.28 g/L (NH4)2SO4, 0.49 g/L KNO3 and 1.19 g/L beef extract. The kinetic characteristics of batch cultivation were investigated in a 5-L pH-stat fermentor. The maximum amount of biomass and highest astaxanthin yield in terms of volume and in terms of biomass were 7.71 mg/L and 1.00 mg/g, respectively. 展开更多
关键词 Phaffia rhodozyma Astaxanthin Nitrogen source Optimization
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Engineering CrtW and CrtZ for improving biosynthesis of astaxanthin in Escherichia coli 认领 引用 被引量：9
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作者 LI Di LI Yang +7 位作者 XU Jiao-Yang LI Qing-Yan TANG Jin-Lei JIA Shi-Ru BI Chang-Hao DAI Zhu-Bo ZHU Xin-Na ZHANG Xue-Li 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2020年第9期666-676,共11页
This study engineered β-carotene ketolase CrtW and β-carotene hydroxylase CrtZ to improve biosynthesis of astaxanthin in Escherichia coli. Firstly, crtW was randomly mutated to increase CrtW activities on conversion... This study engineered β-carotene ketolase CrtW and β-carotene hydroxylase CrtZ to improve biosynthesis of astaxanthin in Escherichia coli. Firstly, crtW was randomly mutated to increase CrtW activities on conversion from β-carotene to astaxanthin. A crtW* mutant with A6 T, T105 A and L239 M mutations has improved 5.35-fold astaxanthin production compared with the wild-type control. Secondly, the expression levels of crtW* and crtZ on chromosomal were balanced by simultaneous modulation RBS regions of their genes using RBS library. The strain RBS54 selected from RBS library, directed the pathway exclusively towards the desired product astaxanthin as predominant carotenoid(99%). Lastly, the number of chromosomal copies of the balanced crtW*-crtZ cassette from RBS54 was increased using a Cre-loxP based technique, and a strain with 30 copies of the crtW*-crtZ cassette was selected. This final strain DL-A008 had a 9.8-fold increase of astaxanthin production compared with the wild-type control. Fed-batch fermentation showed that DL-A008 produced astaxanthin as predominant carotenoid(99%) with a specific titer of 0.88 g·L-1 without addition of inducer. In conclusion, through constructing crtW mutation, balancing the expression levels between crtW* and crtZ, and increasing the copy number of the balanced crtW*-crtZ cassette, the activities of β-carotene ketolase and β-carotene hydroxylase were improved for conversion of β-carotene to astaxanthin with higher efficiency. The series of conventional and novel metabolic engineering strategies were designed and applied to construct the astaxanthin hetero-producer strain of E. coli, possibly offering a general approach for the construction of stable hetero-producer strains for other natural products. 展开更多
关键词 Astaxanthin RBS library Metabolic engineering β-Carotene ketolase Cre-loxP Escherichia coli
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Determination of astaxanthin and astaxanthin esters in shrimp shell by HPLC 认领 引用 被引量：11
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作者 LI Shuai GUO Jun-ru +6 位作者 FENG Jin-hua ZHU Ze-min LI Ling-li GUO Xiao-fang SUI Wen-yan WANG Meng ZUO Wen-ting 《Marine Science Bulletin》 2019年第2期57-71,共15页
A method for determination of astaxanthin and astaxanthin eaters in shrimp shell by high performance liquid chromatography is established.Shrimp shell are addressed with 5%hydrochloric acid to remove calcium ions.Afte... A method for determination of astaxanthin and astaxanthin eaters in shrimp shell by high performance liquid chromatography is established.Shrimp shell are addressed with 5%hydrochloric acid to remove calcium ions.After shell is dried,organics from shrimp shell are extracted with anhydrous ethanol.The alcohol extrative of the shrimp shell is mixed with the ammonium sulfate to extract astaxanthin by aqueous two-phase extraction.The crude astaxanthin is collected,which is distributed in the middle layer of the aqueous two-phase layer.After distilled water is added to the crude astaxanthin,the aqueous solution is centrifuged,and the previous step is repeated for several times.The precipitation in centrifuge tube is collected and dried.The crude astaxanthin dried is dissolved with acetone,and the sample solution is separated by TLC.Every pigment on the TLC plate is collected and dissolved with acetone.The pigments are determined by high performance liquid chromatograph.The results show that aqueous two-phase system,3 mL alcohol extractive of astaxanthin and 4.5 mL 20%ammonium sulfate,can be used to acquire crude astaxanthin.The wavelength of the maximum peak of astaxanthin in ethanol solution is 472 nm.A variety of pigments can be separated from the crude astaxanthin by thin-layer chromatography,including free astaxanthin,astaxanthin monoester,astaxanthin diester,echinenone and other substances.It can be seen from high performance liquid chromatography that the appearance time of free astaxanthin is from 4 min to 5.5 min,and the appearance time of astaxanthin monoester is from 10.5 min to 27.8 min.The method is simple about the sample pretreatment and feasible about the determination of astaxanthin and astaxanthin esters in shrimp shell. 展开更多
关键词 astaxanthin astaxanthin esters aqueous two-phase thin-layer chromatography high performance liquid chromatography
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Efficient Extraction of Astaxanthin from Phaffia rhodozyma with Polar and Non-polar Solvents after Acid Washing 认领 引用 被引量：6
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作者 尹春华 杨淑珍 +1 位作者 刘晓璐 闫海 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2013年第7期776-780,共5页
method of extracting astaxanthin from Phaffia rhodozyma with various solvents after acid washing was investigated. The extraction efficiency was distinctly increased after acid washing of P. rhodozyma cells. When the ... method of extracting astaxanthin from Phaffia rhodozyma with various solvents after acid washing was investigated. The extraction efficiency was distinctly increased after acid washing of P. rhodozyma cells. When the concentration of HCl was 0.4 mol.L^-1, the highest extraction efficiency of astaxanthin was achieved which was about three times higher than the control. Acetone or benzene as single polar or non-polar solvent was the most ef- fective solvent in our research. With a combination of isopropanol and n-hexane （volume ratio of 2 ： 1）, the maxi- mal extraction efficiency was achieved, approximately 60% higher than that obtained with a single solvent. The liquid-solid ratio and the extracting time were also optimized. Under the optimum extraction conditions, the extraction yield of astaxanthin exceeded 98%. 展开更多
关键词 astaxanthin Phaffia rhodozyma acid wash extraction solvent
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Metabolic mechanism of astaxanthin biosynthesis in Xanthophyllomyces dendrorhous in response to sodium citrate treatment 认领 引用 被引量：5
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作者 Xueshan Pan Tonggang Li +7 位作者 Baobei Wang Shuhua Qi Dandan Yang Zheng Huang Renfei Gao Jingyan Li Xueping Ling Yinghua Lu 《Bioresources and Bioprocessing》 SCIE EI 2023年第1期1003-1018,共16页
Astaxanthin is an important ketocarotenoid widely used in industries.However,its application is limited because of its low yield.Sodium citrate(Na-citrate),one of the major carbon sources for microorganisms,can promot... Astaxanthin is an important ketocarotenoid widely used in industries.However,its application is limited because of its low yield.Sodium citrate(Na-citrate),one of the major carbon sources for microorganisms,can promote cell growth and product accumulation.The basidiomycetous red yeast Xanthophyllomyces dendrorhous was thus used to study the effect of Na-citrate on cell growth and astaxanthin synthesis.The highest biomass and astaxanthin yield(6.0 g/L and 22.5 mg/L)were obtained in shake-flask when 3 g/L Na-citrate was added at 24 h and were 1.8 and 2.0 times higher than those of the control group,respectively.Furthermore,metabolomics and real-time reverse transcription PCR(qRT-PCR)analysis were conducted to study the metabolic pathways of X.dendrorhous in response to Na-citrate.The qRT-PCR assay revealed that Na-citrate facilitated glucose consumption,promoted the metabolic flux from glycolysis,and regulated the tricarboxylic acid(TCA)cycle,providing more energy and substrates for the synthesis of astaxanthin.The gene analysis revealed that adding Na-citrate significantly upregulated the expression of six key genes(ICL,HMGS,crtE,crtYB,crtI,and crtS)involved in pathways related to astaxanthin biosynthesis.These results suggest that exogenous Na-citrate treatment is a potentially valuable strategy to stimulate astaxanthin production in X.dendrorhous. 展开更多
关键词 Xanthophyllomyces dendrorhous Astaxanthin Na-citrate Metabolomics Gene expression
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Stability and changes in astaxanthin ester composition from Haematococcus pluvialis during storage 认领 引用 被引量：9
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作者 苗凤萍 耿亚洪 +2 位作者 卢大炎 左进城 李夜光 《Chinese Journal of Oceanology and Limnology》 CSCD 2013年第6期1181-1189,共9页
In this paper,we investigated the effects of temperature,oxygen,antioxidants,and corn germ oil on the stability of astaxanthin from Haematococcus pluvialis under different storage conditions,and changes in the composi... In this paper,we investigated the effects of temperature,oxygen,antioxidants,and corn germ oil on the stability of astaxanthin from Haematococcus pluvialis under different storage conditions,and changes in the composition of astaxanthin esters during storage using high performance liquid chromatography and spectrophotometry.Oxygen and high temperatures(22–25°C) significantly reduced the stability of astaxanthin esters.Corn germ oil and antioxidants(ascorbic acid and vitamin E)failed to protect astaxanthin from oxidation,and actually significantly increased the instability of astaxanthin.A change in the relative composition of astaxanthin esters was observed after 96 weeks of long-term storage.During storage,the relative amounts of free astaxanthin and astaxanthin monoesters declined,while the relative amount of astaxanthin diesters increased.Thus,the ratio of astaxanthin diester to monoester increased,and this ratio could be used to indicate if astaxanthin esters have been properly preserved.If the ratio is greater than 0.2,it suggests that the decrease in astaxanthin content could be higher than 20%.Our results show that storing algal powder from H.pluvialis or other natural astaxanthin products under vacuum and in the dark below 4°C is the most economical and applicable storage method for the large-scale production of astaxanthin from H.pluvialis.This storage method can produce an astaxanthin preservation rate of at least 80%after 96 weeks of storage. 展开更多
关键词 Haematococcus pluvialis astaxanthin ester storage stability storage life high performance liquid chromatography （HPLC）
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Natural astaxanthin enhanced antioxidant capacity and improved semen quality through the MAPK/Nrf2 pathway in aging layer breeder roosters 认领 引用 被引量：5
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作者 Shan Gao Nuo Heng +9 位作者 Fang Liu Yong Guo Yu Chen Liang Wang Hemin Ni Xihui Sheng Xiangguo Wang Kai Xing Longfei Xiao Xiaolong Qi 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2022年第2期578-592,共15页
Background:Natural astaxanthin(ASTA)has strong antioxidant properties and has been widely used as a health product to improve human health.However,the effects of ASTA on the reproductive performance of aging roosters ... Background:Natural astaxanthin(ASTA)has strong antioxidant properties and has been widely used as a health product to improve human health.However,the effects of ASTA on the reproductive performance of aging roosters have been poorly studied.We aimed to investigate the effects of dietary ASTA on semen quality and antioxidant capacity in aging roosters and to explore the potential mechanism of semen quality change via antioxidation defense system.Methods:In the present study,9653-week-old Jinghong No.1 layer breeder roosters were fed a corn-soybean meal basal diet containing 0,25,50,or 100 mg/kg ASTA for 6 weeks.Results:Semen quality in the ASTA groups remarkably improved than that in the control group,and antioxidant activities,the abilities to scavenge hydroxyl radicals and superoxide anions,increased gradually with ASTA addition(P<0.05).In addition,the mRNA levels of antioxidant enzymes as well as the mRNA and protein levels of the mitogen-activated protein kinase(MAPK)and nuclear factor-erythroid 2-related factor 2(Nrf2)were markedly increased in the 50-100 mg/kg ASTA group(P<0.05).Conclusions:Collectively,these results demonstrate that dietary ASTA may improve semen quality by increasing antioxidant enzyme activities and the ability to scavenge hydroxyl radicals,which may be related to upregulation of the MAPK/Nrf2 pathway. 展开更多
关键词 Aging rooster Antioxidant capacity MAPK/Nrf2 pathway Natural astaxanthin Semen quality
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High-level de novo biosynthesis of glycosylated zeaxanthin and astaxanthin in Escherichia coli 认领 引用 被引量：4
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作者 Xixian Chen Xiaohui Lim +2 位作者 Aurélie Bouin Thomas Lautier Congqiang Zhang 《Bioresources and Bioprocessing》 SCIE EI 2021年第1期974-986,共13页
Because of wide applications in food,feed,pharmaceutical and cosmetic industries,the carotenoid market is growing rapidly.Most carotenoids are hydrophobic,which limits their bioavailability.Glycosylation is a natural ... Because of wide applications in food,feed,pharmaceutical and cosmetic industries,the carotenoid market is growing rapidly.Most carotenoids are hydrophobic,which limits their bioavailability.Glycosylation is a natural route that substantially increases the water solubility,as well as the bioavailability,photostability and biological activities of carotenoids.Here,we report metabolic engineering efforts (e.g.,promoter and RBS engineering,optimization of carbon sources and supplementation of bottleneck genes) to produce glycosylated carotenoids in Escherichia coli.By fine-tuning the carotenoid-biosynthetic genes (crtX,crtZ and crtY),our strain produced up to 47.2 mg/L (~ 11,670 ppm) of zeaxanthin glucosides, ~ 78% of the total carotenoids produced.In another construct with mevalonate,astaxanthin pathway and crtX genes,the strain produced a mixture of carotenoid glucosides including astaxanthin and adonixanthin glucosides with a total yield of 8.1 mg/L (1774 ppm).Our work demonstrated a proof-of-concept study for the microbial biosynthesis of glycosylated carotenoids. 展开更多
关键词 Carotenoids Metabolic engineering Synthetic biology Glycosylation Glucosyltransferase Zeaxanthin Astaxanthin UPD-glucose
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