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Precise l-threonine-to-l-isoleucine pathway regulation for engineering high-efficiency whole-cell biocatalysts 认领 引用 被引量：1
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作者 Heng Zhang Fuqiang Song +4 位作者 Ke Wang Faqing Wu Lihao Deng Kun Qiu Jingwen Zhou 《Synthetic and Systems Biotechnology》 SCIE CSCD 2026年第2期1-9,共9页
l-Isoleucine(L-Ile),a critical branched-chain amino acid with diverse applications in food,pharmaceutical,and cosmetic industries,is difficult to produce efficiently at scale in microbial systems due to metabolic bott... l-Isoleucine(L-Ile),a critical branched-chain amino acid with diverse applications in food,pharmaceutical,and cosmetic industries,is difficult to produce efficiently at scale in microbial systems due to metabolic bottlenecks and cofactor limitations.This study metabolically engineered Escherichia coli BL21(DE3)to develop a whole-cell biocatalyst for efficient L-Ile biosynthesis.Key strategies included screening acetohydroxy acid synthase(AHAS)isoenzymes,identifying ilvGM-encoded AHAS II as the optimal enzyme,relieving feedback inhibition of ilvA(encoding l-threonine dehydratase)through mutant screening,and optimizing genetic circuits(promoter tuning,plasmid copy number).Dual-precursor supplementation revealed l-threonine as a critical factor for suppressing l-valine byproduct.Fed-batch fermentation in a 5 L bioreactor achieved a peak molar conversion rate of 98.4%,yielding 40.1 g/L L-Ile within 36 h.The mass conversion rate(L-Ile/glucose)achieved 0.36 g/g and the production efficiency achieved 1.11 g/L/h,demonstrating the feasibility of whole-cell catalysis.This work provides a robust framework for industrial L-Ile production and transferable strategies for branched-chain amino acid pathway optimization. 展开更多
关键词 l-isoleucine production Whole-cell biocatalyst Transcription regulation Metabolic engineering Acetohydroxy acid synthase Dual-precursor supplementation
Laccases as green and versatile biocatalysts:from lab to enzyme market-an overview 认领 引用 被引量：5
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作者 Tatiane Brugnari Dayane Moreira Braga +4 位作者 Camila Souza Almeida dos Santos Bruno Henrique Czelusniak Torres Tatiani Andressa Modkovski Charles Windson Isidoro Haminiuk Giselle Maria Maciel 《Bioresources and Bioprocessing》 SCIE EI 2021年第1期80-108,共29页
Laccases are multi-copper oxidase enzymes that catalyze the oxidation of different compounds (phenolics and non-phenolics).The scientific literature on laccases is quite extensive,including many basic and applied rese... Laccases are multi-copper oxidase enzymes that catalyze the oxidation of different compounds (phenolics and non-phenolics).The scientific literature on laccases is quite extensive,including many basic and applied research about the structure,functions,mechanism of action and a variety of biotechnological applications of these versatile enzymes.Laccases can be used in various industries/sectors,from the environmental field to the cosmetics industry,including food processing and the textile industry (dyes biodegradation and synthesis).Known as eco-friendly or green enzymes,the application of laccases in biocatalytic processes represents a promising sustainable alternative to conventional methods.Due to the advantages granted by enzyme immobilization,publications on immobilized laccases increased substantially in recent years.Many patents related to the use of laccases are available,however,the real industrial or environmental use of laccases is still challenged by cost-benefit,especially concerning the feasibility of producing this enzyme on a large scale.Although this is a compelling point and the enzyme market is heated,articles on the production and application of laccases usually neglect the economic assessment of the processes.In this review,we present a description of laccases structure and mechanisms of action including the different sources (fungi,bacteria,and plants) for laccases production and tools for laccases evolution and prediction of potential substrates.In addition,we both compare approaches for scaling-up processes with an emphasis on cost reduction and productivity and critically review several immobilization methods for laccases.Following the critical view on production and immobilization,we provide a set of applications for free and immobilized laccases based on articles published within the last five years and patents which may guide future strategies for laccase use and commercialization. 展开更多
关键词 Biocatalysts Multi-copper oxidases Ligninolytic enzymes Immobilization Patents
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Construction of the yeast whole-cell Rhizopus oryzae lipase biocatalyst with high activity 认领 引用 被引量：2
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作者 Mei-ling CHEN Qin GUO +4 位作者 Rui-zhi WANG Juan XU Chen-wei ZHOU Hui RUAN Guo-qing HE 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS CSCD 2011年第7期545-551,共7页
Surface display is effectively utilized to construct a whole-cell biocatalyst.Codon optimization has been proven to be effective in maximizing production of heterologous proteins in yeast.Here,the cDNA sequence of Rhi... Surface display is effectively utilized to construct a whole-cell biocatalyst.Codon optimization has been proven to be effective in maximizing production of heterologous proteins in yeast.Here,the cDNA sequence of Rhizopus oryzae lipase (ROL) was optimized and synthesized according to the codon bias of Saccharomyces cerevisiae,and based on the Saccharomyces cerevisiae cell surface display system with α-agglutinin as an anchor,recombinant yeast displaying fully codon-optimized ROL with high activity was successfully constructed.Compared with the wild-type ROL-displaying yeast,the activity of the codon-optimized ROL yeast whole-cell biocatalyst (25 U/g dried cells) was 12.8-fold higher in a hydrolysis reaction using p-nitrophenyl palmitate (pNPP) as the substrate.To our knowledge,this was the first attempt to combine the techniques of yeast surface display and codon optimization for whole-cell biocatalyst construction.Consequently,the yeast whole-cell ROL biocatalyst was constructed with high activity.The optimum pH and temperature for the yeast whole-cell ROL biocatalyst were pH 7.0 and 40 °C.Furthermore,this whole-cell biocatalyst was applied to the hydrolysis of tributyrin and the resulted conversion of butyric acid reached 96.91% after 144 h. 展开更多
关键词 Rhizopus oryzae lipase （ROL） Yeast surface display Codon optimization Whole-cell biocatalyst
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Bioengineering Comamonas testosteroni CNB-1:a robust whole-cell biocatalyst for efficient PET microplastic degradation 认领 引用 被引量：3
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作者 Zhanqing Cao Wei Xia +6 位作者 Shilei Wu Jiale Ma Xiaoli Zhou Xiujuan Qian Anming Xu Weiliang Dong Min Jiang 《Bioresources and Bioprocessing》 SCIE EI 2023年第1期18-27,共10页
The escalating crisis of polyethylene terephthalate(PET)microplastic contamination in biological wastewater treatment systems is a pressing environmental concern.These microplastics inevitably accumulate in sewage slu... The escalating crisis of polyethylene terephthalate(PET)microplastic contamination in biological wastewater treatment systems is a pressing environmental concern.These microplastics inevitably accumulate in sewage sludge due to the absence of effective removal technologies.Addressing this urgent issue,this study introduces a novel approach using DuraPETase,a potent enzyme with enhanced PET hydrolytic activity at ambient temperatures.Remarkably,this enzyme was successfully secreted from Comamonas testosteroni CNB-1,a dominant species in the active sludge.The secreted DuraPETase showed significant hydrolytic activity toward p-NPB and PET nanoplastics.Furthermore,the CNB-1 derived whole-cell biocatalyst was able to depolymerize PET microplastics under ambient temperature,achieving a degradation efficiency of 9%within 7 days.The CNB-1-based whole biocatalysts were also capable of utilizing PET degradation intermediates,such as terephthalic acid(TPA)and ethylene glycol(EG),and bis(2-hydroxyethyl)-TPA(BHET),for growth.This indicates that it can completely mineralize PET,as opposed to merely breaking it down into smaller molecules.This research highlights the potential of activated sludge as a potent source for insitu microplastic removal. 展开更多
关键词 microplastics polyethylene terephthalate biological wastewater treatment systems Comamonas testosteroni CNB comamonas testosteroni whole cell biocatalyst biodegradation sewage sludge
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Bovine serum albumin:An efficient and green biocatalyst for the one-pot four-component synthesis of pyrano[2,3-c]pyrazoles 认领 引用 被引量：1
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作者 Xingtian Huang Zhipeng Li +1 位作者 Dongyang Wang Yiqun Li 《Chinese Journal of Catalysis》 SCIE EI CAS CSCD 北大核心 2016年第9期1461-1468,共8页
The use of biocatalysts is attracting an increasing amount of attention in chemical catalysis.Here,we have shown that bovine serum albumin（BSA）,a ubiquitous,inexpensive,non-enzymatic transport protein,can serve as a... The use of biocatalysts is attracting an increasing amount of attention in chemical catalysis.Here,we have shown that bovine serum albumin（BSA）,a ubiquitous,inexpensive,non-enzymatic transport protein,can serve as an efficient,retrievable catalyst in the one-pot four-component reaction of aryl aldehydes,malononitrile,hydrazine hydrate,and ethyl acetoacetate for the synthesis of pyrano[2,3-c]pyrazoles under mild reaction conditions.The BSA biocatalyst also displayed a high catalytic affinity for acyclic/cyclic ketones to yield the corresponding pyrano[2,3-c]pyrazoles or their spirocyclic variants.The BSA could be used for at least five cycles without serious loss of catalytic activity.This novel,efficient protocol has the merits of high yield,operational simplicity,and a relatively benign environmental impact.Moreover,the method extends the promiscuity of BSA as a biocatalyst. 展开更多
关键词 Bovine serum albumin Biocatalyst Multicomponent reaction Pyrano[2 3-c]pyrazole Biocatalytic promiscuity
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Preparation of Chiral Hydroxy Esters Using Actinobacteria: Biocatalyst Activity of Marine-Derived Micromonosporaand StreptomycesStrains 认领 引用 被引量：1
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作者 Kohji Ishihara Aiko Fujita +6 位作者 Akane Sakiyama Yuko Kobayashi Kaoru Hori Kanako Maruike Noriyoshi Masuoka Nobuyoshi Nakajima Hiroki Hamada 《Open Journal of Applied Sciences》 2013年第1期116-122,共7页
To research the potential ability of marine-derived actinomycetes to act as biocatalysts, 8 Micromonospora strains and 5 Streptomyces strains were screened. Two recommended media (227 and 1076 media) and 2 modified me... To research the potential ability of marine-derived actinomycetes to act as biocatalysts, 8 Micromonospora strains and 5 Streptomyces strains were screened. Two recommended media (227 and 1076 media) and 2 modified media (1076-25% and P-1076-25% media) for liquid culture of these marine-derived actinomycetes were tested. As a result, 2 Micromonospora strains (Micromonospora sp. NBRC107096 and 107097) cultured with the 1076-25% medium and 2 Streptomyces strains (Streptomyces tateyamensis NBRC105048 and Streptomyces sp. NBRC105896) cultured with P-1076-25% medium showed a good growth. The stereoselective reduction of α-keto esters using these 4 actinomycetes was tested. As a result, it was found that these strains had a reducing activity toward various α-keto esters. The introduction of L-glutamate or sucrose as an additive remarkably increased the conversion ratios in the reduction of substrates by the Micromonospora strain. Furthermore, in the presence of L-alanine, Streptomyces tateyamensis NBRC105048 reduced ethyl pyruvate, ethyl 2-oxobutanoate, ethyl 2-oxopentanoate, ethyl 2-oxohexanoate, and ethyl 3-methyl-2-oxobutyrate to the corresponding α-hydroxy ester with a high conversion ratio and with excellent enantiomeric excess. Thus, we found that these marine-derived actinomycetes have great potential to be used as biocatalysts for stereoselective reduction of carbonyl compounds. 展开更多
关键词 Marine Bacteria Micromonospora Streptomyces Biocatalyst Chiral Alcohol
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The Ability of Edible Mushrooms to Act as Biocatalysts: Preparation of Chiral Alcohols Using Basidiomycete Strains 认领 引用
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作者 Kohji Ishihara Yukiko Nishikawa +9 位作者 Mari Kaneko Anna Kinoshita Nozomi Kumazawa Daichi Kobashigawa Kohei Kuroda Masashi Osawa Tatsunori Yamamoto Nobuyoshi Nakajima Hiroki Hamada Noriyoshi Masuoka 《Advances in Microbiology》 CAS 2012年第2期66-71,共6页
To examine the potential ability of edible mushrooms to act as biocatalysts, 19 basidiomycete strains were screened. Modified media (PG, O, and PGO medium) for liquid cultivation of these basidiomycete strains were de... To examine the potential ability of edible mushrooms to act as biocatalysts, 19 basidiomycete strains were screened. Modified media (PG, O, and PGO medium) for liquid cultivation of these basidiomycete strains were designed and tested. Wet cells (>10 g) of 4 basidiomycete strains (Pleurotus salmoneostramineus H7, P. salmoneostramineus H13, Ganoderma lucidum NBRC31863, Flammulina velutipes NBRC31862) were harvested from PGO medium for 7 days. The stereoselective reduction of α-keto esters using the 4 strains was tested. It was found that each of these strains had a reducing activity toward 6 aliphatic α-keto esters. In the presence of L-alanine as an additive, the reduction of ethyl 2-oxobutanoate and ethyl 2-oxopentanoete by P. salmoneostramineus H7 produced the corresponding alcohol with a high conversion ratio and with excellent enantiomeric excess (>99% e.e. (R)). Furthermore, ethyl pyruvate, ethyl 2-oxobutanoate, and ethyl 2-oxopentanoate were predominantly reduced to the corresponding (R)-hydroxy ester (>99% e.e.) by G. lucidum. Thus, we found that these edible mushrooms have great potential to be used as biocatalysts for the stereoselective reduction of carbonyl compounds. 展开更多
关键词 Basidiomycete Edible Mushroom Biocatalyst Chiral Alcohol
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Novel biocatalyst derived from Araujia sericifera latex:Synthesis and applications 认领 引用
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作者 Daniel Sánchez Gabriela Tonetto María Luján Ferreira 《Green Synthesis and Catalysis》 CSCD 2026年第2期188-197,共10页
Biocatalysts with lipase activity are increasingly required in different industrial sectors since they promote a large number of reactions with high selectivity and yield under mild conditions,promoting the developmen... Biocatalysts with lipase activity are increasingly required in different industrial sectors since they promote a large number of reactions with high selectivity and yield under mild conditions,promoting the development of more sustainable processes.Lipases derived from the latex of laticiferous plants are receiving increasing attention because they are easy to obtain and inexpensive.In this work,we have studied the synthesis of biocatalysts with lipase activity through the entrapment of microparticles of Araujia sericifera latex in a cross-linked chitosan matrix.The effect of the chitosan solution concentration(3-15 mg/ml),the cross-linking agent solution concentration(0-0.5 mol/l),the cross-linking time(0-60 min),the weight ratio latex microparticles/chitosan(1:1-3:1 wt/weight)and the pH value of the capture medium at the time of adding the latex microparticles(3-6)on the synthesis and activity of biocatalysts was studied.The performance of the entrapped microparticles synthesized under optimal conditions was evaluated in hydrolysis,esterification,and transesterification reactions with extremely promising results.This new biocatalyst showed comparable activity to Novozym 435,but its chemical and mechanical stability was considerably higher.It could be a good competitor for this prestigious commercial biocatalyst. 展开更多
关键词 Biocatalyst Lipase Araujia sericifera Hydrolysis Esterification Transesterification
Biotransformation of limonene:pathways,biocatalysts,and applications 认领 引用 被引量：4
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作者 Lulu Zhang Wanying Wang +9 位作者 Zixuan Wang Weimin Song Haoliang Li Jinchu Yang Zhenzhen Huang Gang Fan Jingnan Ren Yujiao Cheng Aiqun Yu Jian Feng 《Food Bioscience》 SCIE 2025年第7期95-114,共20页
Biotransformation of limonene is considered as a promising approach for the production of bioactive compounds.These compounds with excellent aroma characteristics and health benefits,such as perillyl alcohol,carvone,... Biotransformation of limonene is considered as a promising approach for the production of bioactive compounds.These compounds with excellent aroma characteristics and health benefits,such as perillyl alcohol,carvone,andα-terpineol,have great market potential in food,fragrance,cosmetics,pharmaceutical,and agricultural in-dustries.Here,we summarize the products and pathways of limonene biotransformation and highlight the biocatalysts involved in this bioprocess.Numerous microorganisms(e.g.,fungi,bacteria and microalgae),en-zymes isolated from these microorganisms(e.g.,cytochrome P450 enzyme,dehydrogenase,epoxide hydrolase,etc),as well as recombinant cells expressing these enzymes,can degrade and transform limonene into valuable products.Additionally,the extraction and purification of these products from fermentation broth,and their industrial applications are introduced.Finally,the future prospects of limonene biotransformation are discussed. 展开更多
关键词 Limonene Biotransformation Pathway Biocatalyst Application
Engineering a broad-spectrum glucose oxidase via substrate channel and linker design for enhanced lignocellulose bioconversion 认领 引用
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作者 Yong Feng Xihua Chen +6 位作者 Zeyang Li Zhong Ni Zhengfen Wu Zhongjian Guo Fubao Sun Huiqing Chen Huayou Chen 《Synthetic and Systems Biotechnology》 SCIE CSCD 2026年第2期218-228,共11页
Glucose oxidase(GOD)is a widely used enzyme in biotechnology,yet its narrow substrate specificity limits its application in complex bioconversion processes such as agricultural waste valorization.In this study,we empl... Glucose oxidase(GOD)is a widely used enzyme in biotechnology,yet its narrow substrate specificity limits its application in complex bioconversion processes such as agricultural waste valorization.In this study,we employed synthetic biology and protein engineering strategies to engineer a broad-spectrum glucose oxidase from Aureobasidium sp.(AreGOD).Initially,site-directed mutagenesis at N82,a key gatekeeper at the dimer interface,modulated substrate channel geometry,leading to increased catalytic activity towards various sugars,particularly stachyose and xylose.Furthermore,systematic linker engineering between the spore anchor protein CotG and AreGOD revealed that flexible linkers,particularly the(GGGGS)5 repeat(LK3),dramatically expanded the enzyme's substrate spectrum towards various mono-,di-,and oligosaccharides.The optimized spore-displayed AreGOD(CotG-LK3-AreGOD)exhibited strong synergistic effects with cellulase in wheat straw degradation,significantly enhancing the hydrolysis of cellulose,hemicellulose,and lignin.Our work demonstrates an effective and generalizable strategy for engineering substrate-promiscuous oxidases,highlighting the potential of integrative enzyme design for sustainable bioprocessing and agricultural biotechnology. 展开更多
关键词 Glucose oxidase Protein engineering Synthetic biology Bacillus subtilis Whole-cell biocatalyst Lignocellulose bioconversion
Non-canonical bio-inspired iron catalysis for aliphatic C–H functionalization 认领 引用
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作者 Xiao-Hua Chen Yifan Fan +1 位作者 Zitong Wu Tao Tu 《Chinese Chemical Letters》 SCIE CAS CSCD 2026年第3期4-5,共2页
Cytochromes P450(P450s or CYPs)constitute a largesuperfamily of heme-thiolate monooxygenases that are ubiquitous in nature.These remarkable enzymes serve as highly versatile biocatalysts capable of mediating selective... Cytochromes P450(P450s or CYPs)constitute a largesuperfamily of heme-thiolate monooxygenases that are ubiquitous in nature.These remarkable enzymes serve as highly versatile biocatalysts capable of mediating selective oxidations across an exceptionally broad range of organic substrates. 展开更多
关键词 organic substrates aliphatic C H functionalization mediating selective oxidations cytochromes p p s biocatalysts cytochromes P iron catalysis bioinspired
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Asymmetric Syntheses Aided by Biocatalysts 认领 引用 被引量：1
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作者 陈沛然 顾建新 +3 位作者 魏志亮 韩世清 李祖义 林国强 《Chinese Journal of Chemistry》 SCIE CAS 2003年第8期983-993,共11页
This article summarizes the achievements of the authors' group in the area of biocatalyst catalyzed organic reactions in recent 10 years. A strain of Geotrichum sp. obtained by screening is capable of stereoselect... This article summarizes the achievements of the authors' group in the area of biocatalyst catalyzed organic reactions in recent 10 years. A strain of Geotrichum sp. obtained by screening is capable of stereoselectively reducing a number of carbonyl compounds. In many cases, the stereochemistry is complementary with that obtained by baker's yeast. Therefore, this micro organism provides a useful pathway to the preparation of alcohol compounds with specific configurations. On the other hand, a number of plant sources have been screened for oxynitrilases and the hydrocyanation reactions of various arylcarboxaldehydes have been investigated. A “micro aqueous reaction system' was invented, by which a series of novel optically active cyanohydrins were prepared. On this basis, a high through put continuous reaction system has been designed. This paper also describes examples of the syntheses of bio active compounds by using the optically active compounds obtained from the above mentioned catalytic reactions as precursors. 展开更多
关键词 asymmetric synthesis biocatalyst Geotrichum sp. stereoselective reduction oxynitrilase enantioselective hydrocyanation
Degradation of beechwood xylan using food-grade bacteria-like particles displayingβ-xylosidase from Limosilactobacillus fermentum 认领 引用
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作者 Robie Vasquez Ji Hoon Song +4 位作者 Jae Seung Lee Bernadette Bagon Sanghoon Kim Valerie Diane Valeriano Dae-Kyung Kang 《Bioresources and Bioprocessing》 SCIE EI CSCD 2025年第1期1075-1088,共14页
The display of enzymes on bacterial surfaces is an interesting approach for immobilising industrially important biocatalysts.In recent years,non-recombinant surface display using food-grade bacteria,such as lactic aci... The display of enzymes on bacterial surfaces is an interesting approach for immobilising industrially important biocatalysts.In recent years,non-recombinant surface display using food-grade bacteria,such as lactic acid bacteria(LAB),have gained interest because of their safety,simplicity,and cost-effectiveness.β-Xylosidase is one of the many biocatalytic enzymes targeted for immobilisation due to its key role in the complete saccharification of lignocellulosic biomass,including xylan hemicellulose.Recently,the xylose-tolerantβ-xylosidase,LfXyl43,was identified in Limosilactobacillus fermentum.LfXyl43 is capable of producing xylose from the degradation of xylo-oligosaccharides(XOS)and beechwood xylan.This study aimed to immobilise this new biocatalyst on the surface of LAB-derived bacteria-like particles(BLP)and investigate its applicability and reusability in the degradation of xylan hemicellulose.Additionally,the influence of the anchor position and the presence of linker peptides on the display and activity of theβ-xylosidase was investigated.Four expression vectors were constructed to express different anchor-xylosidase fusion proteins.Upon expression and purification,all anchor-xylosidase fusion proteins were active towards the artificial substrate p-nitrophenyl-β-D-xylopyranoside.In addition,all anchor-xylosidase fusion proteins were successfully displayed on the surface of BLP.However,only theβ-xylosidases with linker peptide showed hydrolytic activity after immobilisation on BLP.BLP displayingβ-xylosidases demonstrated high activity against XOS and beechwood xylan,thereby producing high amounts of xylose.Moreover,the immobilised enzyme demonstrated reusability across several bioconversion cycles.Overall,this study highlights the potential industrial application of surface-displayedβ-xylosidase for the effective degradation of lignocellulosic biomass. 展开更多
关键词 Surface display Beta-xylosidase Lactic acid bacteria Xylan Immobilisation Biocatalyst
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中国生物制造关键技术进展与未来趋势 认领 引用 被引量：8
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作者 云慧敏 陈必强 谭天伟 《科技导报》 CAS CSCD 北大核心 2025年第23期24-32,共9页
生物制造作为实现绿色低碳和可持续发展的重要技术路径,正沿着“原料—技术—过程—产品”全产业链加速演进。首先,从原料端分析了传统粮食原料的局限性,重点介绍生物制造原料向非粮生物质拓展,高性能菌种及酶的开发向精准化、智能化演... 生物制造作为实现绿色低碳和可持续发展的重要技术路径,正沿着“原料—技术—过程—产品”全产业链加速演进。首先,从原料端分析了传统粮食原料的局限性,重点介绍生物制造原料向非粮生物质拓展,高性能菌种及酶的开发向精准化、智能化演进,过程工程技术及核心装备向模块化、智能化迭代的最新进展,为构建安全、稳定的生物制造原料基础提供支撑。技术端系统梳理了基因编辑、代谢工程、计算设计、人工智能(AI)等底层工具在核心菌种、底盘细胞和工业酶精准设计中的应用,推动生物制造向高效化、模块化、智能化发展。在过程端,重点讨论智能细胞工厂、精准生物发酵、在线监测、数字孪生及智能装备国产化等关键技术的融合创新,加速实现由经验驱动向模型驱动与智能决策转型。在产品端,总结生物制造在医药、食品、化工、材料等领域的产业化进展及应用前景。最后,针对中国生物制造在菌种自主可控、高端装备依赖等方面的短板,提出关键技术攻关方向,为未来生物制造的技术路线与产业发展提供参考。 展开更多
关键词 生物制造 非粮生物质 生物催化剂 先进装备 人工智能
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复合纳米生物催化剂Fe3O4-ZIF-8@ET 2.0的制备及表征 认领 引用
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作者 李攀登 贺雷雨 孙丽慧 《中国油脂》 CAS CSCD 北大核心 2025年第7期112-117,共6页
为提高脂肪酶Eversa Transform 2.0稳定性和利用率,采用共沉淀法将磁性纳米粒子Fe3O4与金属有机框架材料ZIF-8耦合,并使脂肪酶Eversa Transform 2.0固定于复合材料上,制备复合纳米生物催化剂Fe3O4-ZIF-8@ET 2.0。以酶比活... 为提高脂肪酶Eversa Transform 2.0稳定性和利用率,采用共沉淀法将磁性纳米粒子Fe3O4与金属有机框架材料ZIF-8耦合,并使脂肪酶Eversa Transform 2.0固定于复合材料上,制备复合纳米生物催化剂Fe3O4-ZIF-8@ET 2.0。以酶比活力和蛋白负载率为指标,以固定化时间、加酶量及固定化温度为考察因素,对Fe3O4-ZIF-8@ET 2.0的制备工艺进行了单因素实验优化。通过扫描电子显微镜、透射电子显微镜和傅里叶红外光谱仪对复合固定化酶的微观形貌和官能团进行表征。将Fe3O4-ZIF-8@ET 2.0用于催化米糠油脱臭馏出物的酯化反应,考察其催化性能和重复使用性。结果表明:Fe3O4-ZIF-8@ET 2.0制备的最佳工艺条件为固定化时间6 h、加酶量1.5 mL(六水合硝酸锌0.225 g, 2-甲基咪唑0.622 g,磁性纳米粒子Fe3O40.05 g)、固定化温度40℃,在此条件下Fe3O4-ZIF-8@ET 2.0的酶比活力为50.58 U/g,蛋白负载率为86.64%;表征结果表明Eversa Transform 2.0被成功负载到Fe3O4-ZIF-8上;采用Fe3O4-ZIF-8@ET 2.0催化米糠油脱臭馏出物酯化,酯化率达到94.4%,重复使用5次后,相对酶活力达到57.37%。综上,制备的复合纳米生物催化剂Fe3O4-ZIF-8@ET 2.0具有良好的催化性能,表现出较大的工业化应用潜力。 展开更多
关键词 金属有机框架 复合纳米生物催化剂 脂肪酶Eversa Transform 2.0 固定化
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Fe(Ⅱ)/α-酮戊二酸依赖型卤化酶作为生物催化剂的最新研究进展 认领 引用
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作者 梁敏 黄媛林 +1 位作者 罗月宁 杨登峰 《广西科学院学报》 2025年第2期232-245,共14页
生物催化碳氢键(C-H键)活化在药物和精细化学品合成中具有关键作用,而碳卤键(C-X键)的高选择性构建是其重要研究方向。尽管该领域已取得显著进展,但未活化的C(sp3)-H键的选择性卤化仍面临化学惰性突破、区域及立体控制等多重挑战。Fe... 生物催化碳氢键(C-H键)活化在药物和精细化学品合成中具有关键作用,而碳卤键(C-X键)的高选择性构建是其重要研究方向。尽管该领域已取得显著进展,但未活化的C(sp3)-H键的选择性卤化仍面临化学惰性突破、区域及立体控制等多重挑战。Fe(Ⅱ)/α-酮戊二酸(α-KG)依赖型卤化酶通过独特的自由基反应机制,能在温和条件下实现未活化的C(sp3)-H键中心碳的精准卤化,展现出优异的区域、化学和立体选择性,为药物合成提供创新工具。Fe(Ⅱ)/α-KG依赖型卤化酶包括载体蛋白型和独立型两类,其在生物碱、氨基酸和核苷酸等天然产物生物合成中的卤化反应备受关注。本文综述Fe(Ⅱ)/α-KG依赖型卤化酶的基本特性、催化机制和应用研究进展,并探讨利用基因挖掘等生物信息学方法发掘新型Fe(Ⅱ)/α-KG依赖型卤化酶的潜力,旨在为探索Fe(Ⅱ)/α-KG依赖型卤化酶作为生物催化剂在新型卤化物合成途径中的应用提供有价值的参考。 展开更多
关键词 Fe(Ⅱ)/α-酮戊二酸依赖型卤化酶 生物催化剂 卤化反应 生物合成
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Fungal laccase discovered but yet undiscovered 认领 引用 被引量：14
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作者 Komal Agrawal Venkatesh Chaturvedi Pradeep Verma 《Bioresources and Bioprocessing》 SCIE EI 2018年第1期39-50,共12页
Laccases belongs to multinuclear copper-containing oxidase and can act on a variety of aromatic and non-aromatic compounds.Due to their broad substrate specificity,they are considered as a promising candidate in vario... Laccases belongs to multinuclear copper-containing oxidase and can act on a variety of aromatic and non-aromatic compounds.Due to their broad substrate specificity,they are considered as a promising candidate in various industrial and biotechnological sectors.They are regarded as a“Green Tool”/“Green Catalyst”in biotechnology.The present review focuses on structure,reaction mechanism,categories,applications,economic feasibility,limitations,and future prospects of fungal laccases.Thus,this review would help in understanding laccases along with the areas,which has not been focused and requires attention.Since past,immense work has been carried out on laccases:yet,new discoveries and application are ever increasing which includes bio-fuel,bio-sensor,fiber board synthesis,bioremediation,clinical,textile industry,food,cosmetics,and many more.Hence,it can be stated that fungal laccase is an enzyme which is“discovered but yet undiscovered”. 展开更多
关键词 Laccase Green tool Biocatalyst Application
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The setereoselective preparation ofβ-keto esters using baker's yeast in the presence of rice wine koji 认领 引用 被引量：4
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作者 Jin Cui Ming Jie Zhang +1 位作者 Xiang Qian Wang Nai Jing Cui 《Chinese Chemical Letters》 SCIE CAS 2008年第3期311-313,共3页
By the addition of rice wine koji,enhancement of the reactivity was observed for the baker's yeast reduction ofβ-keto esters into(S)-β-hydroxy esters with high enantiomeric purity(73-98%).
关键词 Baker's yeast Biocatalyst Rice wine koji β-Keto esters
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Hemoglobin-biocatalyzed Synthesis of Conducting Molecular Complex of Polyaniline and Lignosulfonate 认领 引用 被引量：1
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作者 HU Xing LIU Shenggui +1 位作者 ZHAO Mingming ZOU Guolin 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2008年第6期809-815,共7页
A new biocatalyst route for the synthesis of a conducting polyaniline(PANI)/lignosulfonate(LGS)complex was presented.Four different catalysts such as hemoglobin(Hb),5,10,15,20-tetrakis(meso-hydroxyphenyl)porphyrin,iro... A new biocatalyst route for the synthesis of a conducting polyaniline(PANI)/lignosulfonate(LGS)complex was presented.Four different catalysts such as hemoglobin(Hb),5,10,15,20-tetrakis(meso-hydroxyphenyl)porphyrin,iron(II)tetrasulfophthalocyanine and ferric chloride were used to polymerize aniline in the presence of a natural polyelectrolytes template LGS.The experimental results show that Hb is an effective catalyst in this case and the synthesis is simple,and the conditions are mild in that the polymerization may be carried out in lower pH(1.0-4.0)buffered solution and optimal pH of 2.0.Varying concentrations of aniline,LGS and H2O2 in feed the favorable conditions for the production of PANI were determined.UV-vis absorption,FTIR,elemental analysis,conductivity,cyclic voltammetry and thermogravimetric analyses confirm the formation of thermally stable and electroactive PANI. 展开更多
关键词 hemoglobin polyaniline biocatalyst conductivity lignosulfonate
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Biocatalytic Synthesis of Pyruvate from DL-lactate with Enzymes in Pseudomonas sp. 认领 引用 被引量：1
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作者 Jing Song GU Ping XU Yin Bo QU 《Chinese Chemical Letters》 SCIE CAS 2002年第12期1213-1216,共4页
A novel method of preparing pyruvate from DL-lactate catalyzed by enzymes from a bacterial strain of Pseudomonas sp. SM-6 was proposed. Catalytic processes of cell-free extract enzymes and immobilized enzymes were eva... A novel method of preparing pyruvate from DL-lactate catalyzed by enzymes from a bacterial strain of Pseudomonas sp. SM-6 was proposed. Catalytic processes of cell-free extract enzymes and immobilized enzymes were evaluated. The kinetic data were studied, too. 展开更多
关键词 Pyruvate DL-lactate biocatalyst enzyme Pseudomonas.
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