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Stimuli-responsive,antimicrobial-loaded nanocarriers for oral biofilm control and microbiome restoration 认领 引用
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作者 Ding Chen Dize Li +6 位作者 Linzhu Su Dayuan Wang Yijin Ren Henny Cvan der Mei Linqi Shi Tao Chen Henk J.Busscher 《International Journal of Oral Science》 SCIE CAS CSCD 2026年第1期33-50,共18页
Nanotechnology has provided thousands of novel nano-antimicrobials possessing features uncommon in clinically available antimicrobials.Here,nanocarriers loaded with conventional antimicrobials and responding to enviro... Nanotechnology has provided thousands of novel nano-antimicrobials possessing features uncommon in clinically available antimicrobials.Here,nanocarriers loaded with conventional antimicrobials and responding to environmental changes upon entry into oral biofilms are reviewed.Supra-gingival biofilms are characterized by acidic pH,the presence of bacterial enzymes,and the development of hypoxia in deeper layers.Sub-gingival biofilms are slightly alkaline,with hypoxia occurring over their entire depth.Upon entering biofilms,negatively charged,pH-and/or hypoxia-responsive nanocarriers become positively charged.This charge reversal leads to electrostatic double-layer attraction between positively charged nanocarriers towards negatively charged,waterfilled channel walls in biofilms,enhancing their accumulation in a biofilm.Degradation of bacterial enzyme-responsive nanocarriers causes in-biofilm release of antimicrobial cargo,yielding higher local antimicrobial concentrations than can be achieved through their direct,oral administration without harming soft tissues.Enhanced antibiofilm activity after in-biofilm antimicrobial release from biofilm-responsive micelles and liposomes has been demonstrated in vitro towards single-species Streptococcus mutans and Staphylococcus aureus biofilms or in vivo using specific-pathogen-free rodents inoculated with selected pathogens.This preferential antibacterial activity regulated the microbial composition of ex vivo human oral biofilm towards a more healthy microbiome composition.Although clinical confirmation is limited,the potential benefits of stimuli-responsive,antimicrobial-loaded nanocarriers for oral biofilm control and microbiome restoration are worth further investigation towards clinical translation. 展开更多
关键词 microbiome restoration nanotechnology liposomes bacterial enzymesand stimuli responsive nanocarriers oral biofilm control biofilm responsive micelles antimicrobial loaded nanocarriers
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Microenvironment Responsive Polymeric Nitric Oxide Releasing Micelles for Enhancing Eradication of Methicillin-resistant Staphylococcus aureus Biofilm 认领 引用
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作者 Hui‑Fang Ma Zheng‑Xin Ma +4 位作者 Gang‑Feng Li Yu‑Tong Yang Zhi‑Heng Yang Dan Xing Peng Li 《Chinese Journal of Polymer Science》 SCIE EI CAS CSCD 2026年第7期2167-2176,共10页
Biofilm infections pose a severe threat to global public health owing to their persistent and recalcitrant nature.The physical barrier formed by the biofilm impedes the penetration of antimicrobial agents,leading to a... Biofilm infections pose a severe threat to global public health owing to their persistent and recalcitrant nature.The physical barrier formed by the biofilm impedes the penetration of antimicrobial agents,leading to a significantly reduced efficacy of conventional antibiotics.Herein,we developed a polymeric micelle system that responds to the biofilm microenvironment to release nitric oxide(NO),which is capable of disrupting biofilms,thereby enhancing the bactericidal efficacy of antibiotics against embedded bacteria.The hydrophobic small-molecule NO donor was first conjugated to a diblock copolymer composed of N-hydroxyethyl acrylamide and N-acryloyl morpholine to yield an amphiphilic diblock copolymer.This amphiphilic copolymer then self-assembles into polymeric NO-releasing micelles(PNOM).Upon exposure to thiol-containing molecules in the reducing biofilm microenvironment,PNOM responsively released NO in a sustained manner over several days.In vitro studies have demonstrated that PNOM significantly potentiated the anti-biofilm efficacy of levofloxacin(Lev)against methicillin-resistant Staphylococcus aureus(MRSA).The combination of PNOM and Lev dispersed 85.3%of the biofilm biomass and eradicated 98.8%of the embedded bacteria.Moreover,in a murine model of implant-associated MRSA biofilm infection,PNOM was validated to enhance the antibiofilm efficacy of Lev in vivo,achieving a bactericidal rate of 93.9%for MRSA biofilms and significantly alleviating inflammation.In summary,we designed a polymeric micelle system that triggers NO release in response to a thiol-rich biofilm microenvironment,thereby disrupting biofilm formation and enhancing the antibiofilm effect of antibiotics against MRSA.This approach represents a promising therapeutic strategy for treating stubborn biofilm-associated infections. 展开更多
关键词 Polymeric Micelles Nitric oxide Drug-resistant bacteria Biofilm infection
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Role of bpfA in adhesion and biofilm formation of Shewanella putrefaciens CN32 under cold stress:a comprehensive transcriptomic analysis 认领 引用
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作者 Zhenghao Guo Ruheng Xiong +1 位作者 Jun Yan Jing Xie 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第1期125-135,共11页
As a specific spoilage organism of seafood under refrigerated temperature conditions,Shewanella spp.tend to form biofilms that exacerbate the occurrence of seafood spoilage.Biofilm-promoting factor A(BpfA)has been rep... As a specific spoilage organism of seafood under refrigerated temperature conditions,Shewanella spp.tend to form biofilms that exacerbate the occurrence of seafood spoilage.Biofilm-promoting factor A(BpfA)has been reported to promote the adhesion and biofilm formation of Shewanella spp.,but its role in adhesion and biofilm formation of S.putrefaciens under cold stress needs to be further investigated.To better comprehend the effect of BpfA on adhesion and biofilm formation of S.putrefaciens under cold stress(4℃),bacterial adhesion and biofilm phenotype of S.putrefaciens CN32 WT andΔbpfA at 4℃were analyzed and performed transcriptomics.The results showed that the deletion of bpfA had almost no effect on the growth of S.putrefaciens CN32 at 4℃,but weakened the unicellular adhesion capacity of S.putrefaciens CN32 and destabilized the stability of the multicellular adhesion layer.In addition,the biomass of the mature biofilm formed byΔbpfA was merely around 50%of that observed in the mature biofilm of S.putrefaciens CN32 WT,the average thickness and volume of the biofilm decreased by 18%and 27%,respectively,and the composition of the biofilm changed.Transcriptome analysis demonstrated that the deletion of bpfA led to differential expression of genes involved in metabolic pathways such as bacterial chemotaxis,two-component system,tyrosine metabolism,drug metabolism-other enzymes and biofilm formation-Vibrio cholerae,which in turn influenced bacterial adhesion and biofilm formation.Those results advance our acknowledgment of the character of BpfA on adhesion and biofilm formation of S.putrefaciens CN32,which contributes to understanding bacterial adhesion and the control of biofilm formation. 展开更多
关键词 Shewanella putrefaciens Cold stress BpfA Adhesion Biofilm Transcriptomics
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Chitosan/LDH surface-modified polyurethane carriers:enhanced biofilm formation,stability,and nitritation reactor performance 认领 引用
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作者 Shuyun Liu Xuewen Gao +5 位作者 Jiaqi Liu Shoujun Yuan Jingwei Feng Chunhua He Wei Wang Zhen-Hu Hu 《ENGINEERING Environment》 SCIE EI CAS CSCD 2026年第4期1-17,共17页
Ammonia-oxidizing bacteria(AOB)are slow-growing autotrophs prone to washout.Biofilm carriers improve retention;however,conventional types suffer from low roughness,poor hydrophilicity,and unfavorable surface charge,li... Ammonia-oxidizing bacteria(AOB)are slow-growing autotrophs prone to washout.Biofilm carriers improve retention;however,conventional types suffer from low roughness,poor hydrophilicity,and unfavorable surface charge,limiting biofilm formation.In this study,a composite carrier was fabricated by loading chitosan(CS)and layered double hydroxide(LDH)onto a polyurethane(PU)sponge,which introduced hydroxyl(-OH)and amino(-NH2)functional groups as well as Mg2+and Al3+ions onto the surface,increased surface roughness,and enhanced the carrier hydrophilicity by 29.2%.During a 55-d nitritation biofilm cultivation experiment,the carrier modified with 0.8 wt%LDH and 0.8 wt%chitosan exhibited significantly enhanced performance.Compared to the unmodified carrier,the sludge adsorption capacity increased by 80.3%,the biofilm biomass increased by 46.8%,and the biofilm growth rate increased by 198.3%,reaching 333.4±9.5 mg/carrier,2023.2±31.5 mg/carrier,and 103.2 mg/(carrier·d)respectively.In addition,the biofilm stability on the carrier was significantly enhanced,with a 54.1%reduction in sludge detachment under ultrasound treatment compared with the unmodified carrier.The nitritation reactor with the CS/LDH-PU carrier maintained stable nitritation performance under high ammonia loading(1.0 g/(L·d))and a higher sludge concentration(5.5 g/L),while the reactor without the carrier collapsed at a lower sludge concentration(4.6 g/L).These findings suggest that the CS/LDH-PU carrier provides an effective strategy for optimizing conventional nitritation carriers and enhancing the resilience of nitritation systems under high ammonia load conditions. 展开更多
关键词 Ammonia-oxidizing bacteria Biofilm carrier Chitosan(CS) Layered double hydroxides(LDH) Nitritation performance
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Monitoring oil pipeline microbiologically influenced corrosion(MIC)and its mitigation using a biofilm/MIC test kit 认领 引用
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作者 Lingjun Xu Adnan Khan +1 位作者 Sarah A.Aqeel Tingyue Gu 《Petroleum Science》 SCIE EI CAS CSCD 2026年第1期486-495,共10页
Microbiologically influenced corrosion(MIC)is caused by microbial biofilms.In this work,an oilfield produced water sample was analyzed using a newly developed disposable electrochemical biofilm/MIC test kit consisting... Microbiologically influenced corrosion(MIC)is caused by microbial biofilms.In this work,an oilfield produced water sample was analyzed using a newly developed disposable electrochemical biofilm/MIC test kit consisting of two solid-state electrodes in a 10 mL standard serum vial for assessing biofilm growth,biocorrosivity and biocide treatment efficacy.The produced water sample was found to be low in microbial cell counts and nutrients.To simulate a possible worst-case scenario,the produced water sample was subcultured at 37°C using enriched artificial seawater(EASW)for 3 rounds before being used as the seed culture for further MIC and biocide tests.The electrochemical test results from the10 mL biofilm/MIC test kit including polarization resistance(Rp)from linear polarization resistance scans and corrosion current density(icorr)from Tafel scans indicated a corrosion rate sequence of no biocide treatment>20 ppm(w/w)tetrakis hydroxymethyl phosphonium sulfate(THPS)>50 ppm THPS.Rp was able to predict biofilm maturity time using the incubation time when Rp leveled off(i.e.,time to reach maximum corrosivity).Two common electron transfer promotors were found to accelerate MIC in the test kit vial injection tests,pointing to extracellular electron transfer-MIC as the main mechanism.This observation was consistent with the 30%corrosive sulfate reducers among all microbes in the mixed culture sample found by metagenomics.In the coupon incubation tests in 125 mL anaerobic vials,the 7-d X60 carbon steel weight loss was 1.1±0.2 mg/cm2(2.9 mpy uniform corrosion rate)without biocide treatment.With 20 ppm THPS biocide in EASW,it dropped to 0.5±0.2 mg/cm2(1.3 mpy),and with 50 ppm THPS,it became negligible.The corresponding MIC pit depths were 10.5,8.9μm,and no well-defined pits,respectively for the three biocide treatment conditions.The weight loss data confirmed the corrosion rate sequence from the biofilm/MIC test kit.This work presents a new MIC monitoring and biocide treatment assessment system for oilfield applications using the new biofilm/MIC test kit. 展开更多
关键词 MIC test kit Biofilm test kit MIC monitoring Biocide efficacy Electrochemical scan
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Quorum sensing:its roles in mediating biofilm and viable but non-culturable state formation,and strategies for the prevention and control of foodborne bacteria via quorum quenching 认领 引用
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作者 Ting Ding Xuchen Li +3 位作者 Hongwei Zhan Yanqing Li Zhenqing Li Yang Deng 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第2期520-537,共18页
Foodborne bacteria produce biofilms and their viable but non-culturable(VBNC)formation,can affect food quality and safety.Studies have shown that these characteristics are regulated by the bacterial quorum sensing(QS)... Foodborne bacteria produce biofilms and their viable but non-culturable(VBNC)formation,can affect food quality and safety.Studies have shown that these characteristics are regulated by the bacterial quorum sensing(QS)system.Quenching the QS system of foodborne bacteria and blocking the expression of the corresponding genes may be an effective way to improve food quality and safety.Therefore,this article reviews the QS systems for foodborne bacteria,the regulatory mechanisms of QS systems in biofilm and VBNC formation and resuscitation,the research progress on quorum sensing inhibitors(QSIs)for Gram-negative and Gram-positive bacteria,and introduces QSIs from various sources.In addition,we have also summarized the current research issues on QS regulation of biofilms and VBNC formation.The systematic study of the QS phenomenon of foodborne bacteria in practical situations,the mechanism of bacterial QS cooperation-cheating,the screening of novel and highly active QSIs,the combination of QSIs and other technologies to improve their bioavailability,and the regulatory network between biofilm and VBNC formation and resuscitation are research directions that need to be paid attention to in the future. 展开更多
关键词 Foodborne bacteria Quorum sensing Biofilm formation Viable but non-culturable state formation Food quality
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Phage-Inspired Artificial Peroxidases with Robust Sub-Nanometer Cluster Sites for Efficient Oral Biofilm Elimination and Dental Caries Prevention 认领 引用
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作者 Huang Zhu Ting Wang +7 位作者 Shihuan Gao Wei Geng Tian Ma Liang Cheng Xianglong Han Jiuhong Deng Shanshan Gao Chong Cheng 《Nano-Micro Letters》 SCIE EI CAS CSCD 2026年第9期171-190,共20页
Dental caries,a highly prevalent oral disease,is primarily driven by pathogenic biofilms;however,current antimicrobials exhibit limited efficacy and poor specificity against cariogenic biofilms.Although nanobiocatalys... Dental caries,a highly prevalent oral disease,is primarily driven by pathogenic biofilms;however,current antimicrobials exhibit limited efficacy and poor specificity against cariogenic biofilms.Although nanobiocatalysts that can produce reactive oxygen species represent a promising alternative to conventional antimicrobials,most current designs fail to achieve robust bacterial interaction and exhibit insufficient disruption of biofilm integrity.To address these challenges,we report the de novo design of phage-inspired artificial peroxidases(IrNC@TiO2)featuring a robust sub-nanometer cluster site and urchin-like topography,which enables efficient oral biofilm elimination and dental caries prevention.Structural characterization confirmed that sub-nanometer Ir clusters are stably anchored to the TiO2support via Ir–O coordination.Leveraging the robust enzymatic activity of Ir clusterzymes and the topological advantages of the spiky substrate,IrNC@TiO2exhibits potent multi-enzyme mimetic activity,generating substantial amounts of·O2-and HClO to effectively capture and eradicate planktonic Streptococcus mutans and suppress biofilm formation.In a caries model,IrNC@TiO2significantly inhibited tooth surface biofilm development,prevented enamel demineralization,and reduced caries incidence.The material also demonstrated negligible cytotoxicity and outperformed conventional non-abrasive additives in tooth-whitening assays.This work introduces a robust and efficient ROS-generating platform for oral health care and proposes a promising solution for clinical caries prevention. 展开更多
关键词 Bioinspired design Artificial peroxidases ROS generation Biofilm eradication Dental caries
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Inhibitory effects of biofilms on the adsorption of per-and polyfluorinated alkyl substances by biological activated carbon in practical drinking water treatment plants 认领 引用
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作者 Zixin Pan Zhenguo Qi +4 位作者 Panyue Zhang Yao Zhang Wenxian Wang Xin Huang Baoyou Shi 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2026年第2期330-335,共6页
Although activated carbon filters are thought to be the best way to remove per-and polyfluorinated alkyl substances(PFAS),it is yet unknown how biofilms affect PFAS removal.This study first examined how the removal of... Although activated carbon filters are thought to be the best way to remove per-and polyfluorinated alkyl substances(PFAS),it is yet unknown how biofilms affect PFAS removal.This study first examined how the removal of PFAS in full-scale drinking water treatment plants(DWTPs)was impacted by biofilm from biological activated carbon(BAC)of varying depths and carbon ages.PFAS desorption from BAC was visible,but at this point,BAC could still remove dissolved organic matter(DOM)efficiently.Studies have demonstrated that the use of activated carbon filters can dramatically lower the content of PFAS in water,with the amount of PFAS reducing as the filter’s depth grows and its use duration increases.Additionally,pore-clogging becomes more noticeable as the biofilm ages,which reduces BAC’s capacity to eliminate PFAS and hinders PFAS desorption.Furthermore,the adsorption process of PFAS may be impeded by the secretion of biofilms,which are composed of proteins and polysaccharides.Based on the analysis above,it can be the adsorption of PFAS by BAC is significantly inhibited by biofilms,according to another research.This provides theoretical direction for improving the removal effectiveness of PFAS in DWTPs. 展开更多
关键词 Drinking water Biofilm Biological activated carbon Per-and polyfluorinated alkyl substances
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Simultaneous removal of manganese and nitrate by manganese redox driven moving bed biofilm reactor:Characteristic,biological community and mechanism 认领 引用
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作者 Tenghao Li Weiwu Shen +3 位作者 Yiyang Zhou Sicheng Shao Xiangwei Wu Dandan Pan 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2026年第3期858-871,共14页
This study investigated the effects of different manganese forms(MnCl2and MnO2)on the nitrogen and phosphorus removal performance of moving bed biofilm reactor(MBBR).Compared to the control without manganese,the... This study investigated the effects of different manganese forms(MnCl2and MnO2)on the nitrogen and phosphorus removal performance of moving bed biofilm reactor(MBBR).Compared to the control without manganese,the addition of MnCl2and MnO2increased NO3--N removal efficiency by 11.47%and 9.54%,and total nitrogen(TN)removal efficiency by 17.91%and 15.45%,respectively.The average accumulation of NO2--N decreased from 3.02 to 0.04 mg/L and 0.18 mg/L,respectively.The manganese redox system induced by MnCl2enhanced total phosephorus(TP)removal efficiency by approximately 3.5 times,while MnO2reduced TP removal efficiency by 2.94%.After discontinuing MnCl2and MnO2supplementation,denitrification efficiency significantly declined,and Mn(Ⅱ)reduced by BioMnOx could not sustain the manganese cycling process long-term.Extracellular polymeric substances(EPS)analysis revealed that MnCl2and MnO2stimulated the production of uronic acids,amideⅢ,and secondary amides in proteins.High-throughput sequencing indicated that Proteobacteria,Bacteroidetes,Chloroflexi,and Acidobacteria were the dominant phyla involved in denitrification,but different manganese sources altered the microbial community composition.The relative abundance of Proteobacteria generally decreased,while Bacteroidetes increased by 16.29%and 4.14%with MnCl2and MnO2.MnCl2was more conducive to the Bacteroidetes growth.This study provides a practical framework for applying manganeseenhanced MBBR system in wastewater treatment plants to improve nitrogen removal efficiency and operational stability. 展开更多
关键词 Denitrification Manganese redox cycling Moving bed biofilm reactor Microbial community Extracellular polymeric substances
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Developing HMME-Conjugated NIR-ⅡActivated Carbon Dots Nanospray for the Treatment of MRSA Biofilm Infections in Diabetic Wound via PTT/CDT/SDT 认领 引用
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作者 Liang Zhou Ning Wang +6 位作者 Zumeng Wang Pingping Zhang Yuqin Chu Yingguang Liu Wen Liu Chengwu Zhang Lili Yan 《Rare Metals》 SCIE EI CAS CSCD 2026年第4期405-420,共16页
Diabetic wound infections are a common complication of diabetes,which severely impact patients'quality of life.Effective treatment of diabetic wound infections remains one major challenge in the clinic,partially d... Diabetic wound infections are a common complication of diabetes,which severely impact patients'quality of life.Effective treatment of diabetic wound infections remains one major challenge in the clinic,partially due to the formation of bacterial biofilm and antibiotic resistance.It is imperative to develop non-antibiotic-dependent strategies to efficiently eradicate biofilm infections in diabetic wounds.In this work,an innovative nanospray(CDs-HM)was successfully prepared by linking one acoustic sensitizer(hematoporphyrin monomethyl ether,HMME)to CDs fabricated with hemin,nickel(Ⅱ)chloride,and polymer ethylene imine.CDs-HM exhibited catalase-enhanced sonodynamic properties and photothermal-enhanced chemodynamic properties.In vitro experiments and transcriptomic analysis demonstrated that CDs-HM successfully killed the bacteria and destroyed bacterial biofilm by disrupting bacterial cell membrane integrity,inducing oxidative stress,and inhibiting ATP production through synergistic photothermal therapy/chemodynamic therapy/sonodynamic therapy(PTT/CDT/SDT)effects.In the diabetic wound infection mouse model,CDs-HM remarkably eradicated MRSA biofilm,reduced inflammation levels,and promoted angiogenesis/collagen deposition so as to accelerate wound healing.It is noteworthy that CDs-HM displayed superior bacteria killing and wound healing effects compared with conventional vancomycin and nanosilver dressing interventions.The practicality and effciency of CDs-HM endowed it with broad clinical translation prospects.It paved the way for developing novel strategies for combating diabetic wound infections. 展开更多
关键词 bacterial biofilm carbon dots diabetic infections hematoporphyrin monomethyl ether synergistic therapy wound healing
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Biofilm-state Bifidobacterium adolescentis Gr19 under dynamic culture system effectively alleviates intestinal barrier dysfunction 认领 引用
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作者 Haoxuan Sun Jing Du +4 位作者 Kaisheng Shen Wenyu Ma Xinjie Diao Qi Liu Guorong Liu 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第1期38-54,共17页
Probiotics can regulate gut microbes to maintain human health.However,the sensitivity of probiotics to environmental conditions reduces their bioavailability.In contrast,the formation of probiotic biofilm provides a n... Probiotics can regulate gut microbes to maintain human health.However,the sensitivity of probiotics to environmental conditions reduces their bioavailability.In contrast,the formation of probiotic biofilm provides a natural physical barrier against external interference.Our previous study established a dynamic culture system of the biofilm-state Bifidobacterium adolescentis Gr19(B-DC-B.adolescentis Gr19),forming higher density and more structurally stable biofilms,which enhanced its potential probiotic properties in vivo.Thus,the protective effect and mechanism of B-DC-B.adolescentis Gr19 on lipopolysaccharide(LPS)-induced intestinal barrier dysfunction were investigated in this study.The results showed that B-DC-B.adolescentis Gr19 not only had high resistance and adhesion activity,but also improved the intestinal barrier by increasing goblet cells and promoting the expression of tight junction(TJ)-related proteins.Moreover,B-DC-B.adolescentis Gr19 effectively attenuated intestinal barrier injury in Caco-2 cells by improving intestinal permeability and integrity.Remarkably,B-DC-B.adolescentis Gr19 enhanced expression of TJ proteins,restored localization of cytoskeleton and reduced intestinal inflammation by suppressing the Ras homolog family member A/Rho-associated coiled-coil-forming kinasesuclear factor kappa B/myosin light chain kinase/myosin light chain(RhoA/ROCK/NF-κB/MLCK/MLC)pathway.Therefore,B-DC-B.adolescentis Gr19 plays a key role in mitigating LPS-induced intestinal barrier dysfunction.Overall,the present study provides a theoretical basis for ameliorating intestinal barrier dysfunction and developing novel functional foods by using biofilm-state probiotics under dynamic culture. 展开更多
关键词 Biofilm Bifidobacterium adolescentis Dynamic culture system Caco-2 cell In vivo Intestinal barrier dysfunction
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Microwave-Actuated CuS/CuCo2O4Heterojunction Microneedles for Synergistic Eradication of Deep-Seated Biofilms and Inflammatory Microenvironment Remodeling in Acne Treatment 认领 引用
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作者 Bingran Qi Chang Zheng +1 位作者 Shifeng Jin Kezhu Li 《Rare Metals》 SCIE EI CAS CSCD 2026年第7期627-643,共17页
Deep skin infections and dense biofilm barriers caused by Propionibacterium acnes(P.acnes)remain significant challenges in recurrent acne vulgaris,exacerbated by the poor stratum corneum penetration of conventional th... Deep skin infections and dense biofilm barriers caused by Propionibacterium acnes(P.acnes)remain significant challenges in recurrent acne vulgaris,exacerbated by the poor stratum corneum penetration of conventional therapies and rising antibiotic resistance.Herein,we report a microwave(MW)-responsive microneedle(MN)patch integrated with CuS/CuCo2O4heterojunction nanoparticles for targeted acne therapy.The MN array mechanically breaches the stratum corneum for precise localized delivery,whereas MW irradiation-leveraging its excellent deep-tissue penetration-induces efficient interfacial charge transfer within the heterojunction to generate abundant reactive oxygen species(ROS)in situ.This system achieves over 99%antibacterial efficiency against both Staphylococcus aureus(S.aureus)and P.acnes,effectively eradicating mature biofilms through synergistic physical and biochemical mechanisms.Furthermore,the localized release of Cu2+and Co2+ions demonstrates enzyme-like ROS-scavenging capabilities,which activate the NRF2 antioxidant and autophagy-lysosomal pathways while suppressing the NLRP3/ASC/Caspase-1 inflammasome.Transcriptomic analysis confirms the downregulation of the NF-κB signaling pathway,promoting a microenvironmental transition from a pro-inflammatory to a pro-repair state.Consequently,this transition facilitates fibroblast migration,angiogenesis,and subsequent tissue remodeling.Ultimately,this MW-driven interfacial engineering strategy overcomes the penetration limitations of traditional treatments,presenting a highly promising platform for deep-tissue infection management. 展开更多
关键词 acne therapy antibacterial microneedle biofilm eradication inflammatory microenvironment remodeling MW-responsive heterojunction
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Ultrasound/magnetic field dual-responsive Fe3O4/glucose oxidase catalytic microbubbles for enhanced bacterial biofilm elimination 认领 引用
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作者 Chi Zhang Dou Wang +6 位作者 Liang Lu Feng-Jiao Xu Fan-Sen Xu Xuan Wu Xiao-Xiao Xu Xiao Li Li-Hui Yuwen 《Biomedical Engineering Communications》 CAS 2026年第1期4-12,共9页
Background:The bacterial biofilm poses a significant challenge to traditional antibiotic therapy.There is a great need to develop novel antibiofilm agents combined with biofilm disrupting and bacteria-killing without ... Background:The bacterial biofilm poses a significant challenge to traditional antibiotic therapy.There is a great need to develop novel antibiofilm agents combined with biofilm disrupting and bacteria-killing without the dependence of antibiotic.Methods:Herein,we prepared ultrasound/magnetic field-responsive ferroferric oxide nanoparticles(Fe3O4)/glucose oxidase microbubbles(FGMB)to form a cascade catalytic system for effective removing methicillin-resistant Staphylococcus aureus biofilms.FGMB were prepared through interfacial self-assembly of Fe3O4 nanoparticles(NPs)and glucose oxidase(GOx)at the gas-liquid interface stabilized by surfactants.Under ultrasound/magnetic field stimulation,FGMB disrupted biofilm architecture through microbubble collapse-induced microjets and magnetically driven displacement.Simultaneously,ultrasound-triggered rupture of FGMB released GOx and Fe3O4 NPs.Glucose can be oxidized by GOx to generate gluconic acid and hydrogen peroxide which was subsequently catalyzed into hydroxyl radicals by Fe3O4 NPs,enabling chemical eradication of biofilm-embedded bacteria.Results:Optical microscopy images demonstrated that FGMB have spherical structure with average size of approximately 17μm.FGMB showed a 65.4%decrease in methicillin-resistant Staphylococcus aureus biofilm biomass and 1.1 log bacterial inactivation efficiency(91.2%),suggesting effective biofilm elimination.In vitro experimental results also indicate that FGMB have good biocompatibility.Conclusion:This antibiofilm strategy integrated dual modes of physical biofilm disruption with chemical bacteria-killing shows great potential as a versatile,non-resistant strategy for bacterial biofilm elimination. 展开更多
关键词 bacterial biofilm ultrasound magnetic field catalytic microbubbles mechanical disruption chemical degradation
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Detection of intracellular bacterial communities and biofilms in urinary tract infections with Escherichia coli using staining protocols 认领 引用
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作者 Swathi Pandey Arul Senghor Kadalangudi Aravaanan +1 位作者 Emmanuel Bhaskar Santhi Silambanan 《World Journal of Experimental Medicine》 2026年第2期48-60,共13页
BACKGROUND Urinary tract infections(UTIs)are prevalent worldwide,and Escherichia coli(E.coli)is the most common causative agent.The ability of the bacteria to form intracellular bacterial communities(IBCs)and biofilm ... BACKGROUND Urinary tract infections(UTIs)are prevalent worldwide,and Escherichia coli(E.coli)is the most common causative agent.The ability of the bacteria to form intracellular bacterial communities(IBCs)and biofilm is a major reason for UTIs.Studies have indicated that the persistence of uropathogenic E.coli as IBCs and biofilms has been implicated in UTIs.However,IBCs are not routinely identified by standard diagnostic methods.AIM To compare the various staining techniques for the detection of IBCs in urine samples from E.coli culture-positive UTI patients with the biofilm-forming capability of the isolates.METHODS The study included 73 patients with E.coli culture-confirmed UTI.Before antibiotic treatment midstream urine sample was collected,and the sediment was obtained by centrifugation.The samples were visualized using Sternheimer-Malbin,Wright-Giemsa,Safranin,and immunofluorescence staining to detect IBCs.Formation of biofilms was analyzed by the tube method.Descriptive statistics were used.RESULTS E.coli clusters were seen by light microscopy using various stains.However,immunofluorescence staining showed a better picture in the form of bright intracellular signals,which indicate bacterial aggregates.Biofilm assay showed an association with intracellular colonization.CONCLUSION The various staining techniques help in the identification of uropathogenic E.coli as IBCs inside superficial epithelial cells.These bacteria are also capable of forming biofilms,which resists action of antibiotics.Thus,IBCs and biofilms are rich reservoirs of organisms in the urinary bladder,paving the way for chronic treatment-resistant UTIs.This study requires further larger studies to substantiate these findings. 展开更多
关键词 Urinary tract infections Intracellular bacterial communities Biofilm formation Sustainable Development Goals-3 Escherichia coli
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Antibiofilm potential of lactic acid bacteria:mechanism of cell-free supernatant,metabolite insights,and prospects for edible coating applications in food quality control 认领 引用 被引量：1
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作者 Mohd Fakharul Zaman Raja Yahya Mohd Shafiq Aazmi Muhammad Fareez Ismail 《Food Materials Research》 CAS 2025年第1期178-186,共9页
Biofilms present significant challenges across health,industry,and food safety due to their resistance to conventional antimicrobial strategies.Lactic acid bacteria(LAB)from the gut microbiome exhibit considerable pot... Biofilms present significant challenges across health,industry,and food safety due to their resistance to conventional antimicrobial strategies.Lactic acid bacteria(LAB)from the gut microbiome exhibit considerable potential as natural antibiofilm agents,largely through the bioactive compounds present in their cell-free supernatant(CFS).LAB CFS contains a diverse array of metabolites,such as organic acids,bacteriocins,and biosurfactants,which demonstrate bacteriostatic and bactericidal effects against various biofilm-forming pathogens.However,the specific mechanisms by which LAB CFS disrupts biofilm architecture remain insufficiently understood.This review aims to explore existing research on the antibiofilm efficacy of LAB,with a focus on the roles of CFS metabolites in preventing and disrupting biofilms.Current evidence suggests that LAB metabolites interfere with biofilm formation by targeting bacterial quorum sensing,modifying surface hydrophobicity,and degrading extracellular polymeric substances;further investigation is needed to fully elucidate these pathways.The mechanisms through which LAB-derived compounds impact biofilm integrity,structure,and function are particularly relevant in food preservation and safety.Emerging directions in LAB research also include the development of edible coatings that incorporate LAB CFS for enhanced food quality control.Such edible coatings show the potential to inhibit biofilm-associated spoilage organisms on food surfaces,extending shelf life,and enhancing safety.This work contributes to the field by providing a comprehensive overview of LAB's antibiofilm properties,clarifying metabolitespecific mechanisms,and proposing innovative applications for food industry challenges.It also supports the development of sustainable,natural antimicrobial solutions that align with consumer demand and contribute to food security initiatives. 展开更多
关键词 antibiofilm agentslargely metabolites antibiofilm organic acidsbacteriocinsand bioactive compounds antimicrobial strategieslactic acid bacteria lab lactic acid bacteria biofilm disruption
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Optimization of the Biofilm-Coupled Constructed Wetland Process for Rural Domestic Sewage Treatment 认领 引用
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作者 LI Yongmei LIU Zhulin 《外文科技期刊数据库（文摘版）自然科学》 2026年第2期068-073,共6页
Rural domestic sewage is characterized by decentralized discharge, large water quality fluctuations, low pollutant concentrations, and insufficient centralized treatment facilities, which has become a key source of ru... Rural domestic sewage is characterized by decentralized discharge, large water quality fluctuations, low pollutant concentrations, and insufficient centralized treatment facilities, which has become a key source of rural non-point source pollution and restricts the improvement of rural ecological environment. Traditional single-constructed wetland (CW) processes suffer from low microbial activity, poor nitrogen and phosphorus removal efficiency, and unstable treatment effects under low temperature and low hydraulic load conditions, limiting their large-scale application in rural sewage treatment. To solve the above problems, this study constructed a biofilm-coupled constructed wetland (BF-CW) composite treatment system, optimized key process parameters, including hydraulic retention time (HRT), filler ratio, biofilm carrier dosage, and aeration intensity, and systematically investigated the pollutant removal performance and microbial community characteristics of the optimized system. The results showed that after parameter optimization, the optimal operating conditions of the BF-CW system were determined as a HRT of 12 h, a composite filler (zeolite: vermiculite: gravel = 3:2:5), a biofilm carrier dosage of 15%, and an intermittent aeration intensity of 0.6 m³/(m²·h). Under optimal conditions, the average removal efficiencies of chemical oxygen demand (COD), ammonia nitrogen (NH₄⁺-N), total nitrogen (TN) and total phosphorus (TP) in rural domestic sewage reached 89.24%, 92.17%, 78.35%, and 85.62%, respectively, which were 18.36%, 22.45%, 26.71%, and 20.18% higher than those of the traditional single constructed wetland. Microbial high-throughput sequencing analysis indicated that biofilm coupling significantly enriched the functional microorganisms related to nitrogen and phosphorus removal in the system, including Nitrosomonas, Nitrospira, Denitratisoma, and Polyphosphoribacter, improving the functional stability of the sewage treatment system. 展开更多
关键词 Rural domestic sewage Biofilm coupling Constructed wetland Process optimization Pollutant removal Microbial community
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Advancing Nanotechnology:Targeting Biofilm-Forming Bacteria with Antimicrobial Peptides 认领 引用 被引量：1
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作者 Julia Valladares Campos Janaína Teixeira Costa de Pontes +2 位作者 Christian Shleider Carnero Canales Cesar Augusto Roque-Borda Fernando Rogério Pavan 《Biomedical Engineering Frontiers》 EI CAS 2025年第1期509-531,共23页
Nanotechnology offers innovative solutions for addressing the challenges posed by biofilm-forming bacteria,which are highly resistant to conventional antimicrobial therapies.This review explores the integration of pha... Nanotechnology offers innovative solutions for addressing the challenges posed by biofilm-forming bacteria,which are highly resistant to conventional antimicrobial therapies.This review explores the integration of pharmaceutical nanotechnology with antimicrobial peptides(AMPs)to enhance the treatment of biofilmrelated infections.The use of various nanoparticle systems—including inorganic/metallic,polymeric,lipid-based,and dendrimer nanostructures—provides promising avenues for improving drug delivery,targeting,and biofilm disruption.These nanocarriers facilitate the penetration of biofilms,down-regulate biofilm-associated genes,such as ALS1,ALS3,EFG1,and HWP1,and inhibit bacterial defense mechanisms through membrane disruption,reactive oxygen species generation,and intracellular targeting.Furthermore,nanoparticle formulations such as NZ2114-NPs demonstrate enhanced efficacy by reducing biofilm bacterial counts by several orders of magnitude.This review highlights the potential of combining nanotechnology with AMPs to create novel,targeted therapeutic approaches for combatting biofilm-related infections and overcoming the limitations of traditional antimicrobial treatments. 展开更多
关键词 pharmaceutical nanotechnology enhance treatment biofilmrelated antimicrobial therapiesthis nanotechnology antimicrobial peptides amps drug delivery antimicrobial peptides biofilm
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A mitochondria-targeted aggregation-induced emission photosensitizer for eradication Candida biofilms and treating oral ulcer 认领 引用 被引量：2
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作者 Kun-Mei Liu Yun Wang +3 位作者 Feng-Wei Xia Shun Feng Xiao-Qi Yu Ming-Yu Wu 《Smart Molecules》 CSCD 2025年第3期1-12,共12页
With increasing drug resistance,Candida infections have posed serious threats to public health.Photodynamic therapy harnesses light to destroy pathomycete,providing a smart strategy for combating of Candida infections... With increasing drug resistance,Candida infections have posed serious threats to public health.Photodynamic therapy harnesses light to destroy pathomycete,providing a smart strategy for combating of Candida infections.However,due to lack of organelle targeting ability and bad extracellular polymeric substances penetrability,current photosensitizers(PSs)are far from desirable to clean biofilms and fight against drug resistance.Herein,a mitochondrion targeting aggregationinduced emission PS,LIQ-TPA-TZ,was developed for the efficient photodynamic treatment of oral Candida infection.LIQ-TPA-TZ has good singlet oxygen and hydroxyl radical generation ability,which can efficiently kill the Candida guilliermondii(C.guilliermondii)and eradicate the biofilm.It not only causes mitochondrial damage by disruption of mitochondrial respiratory chain and oxidative stress-related gene but also inhibits fungal adhesion and filamentous growth to prevent Candida colonization,mycelia growth and biofilm formation,which is favorable for eliminating the potential drug resistance.In the mouse oropharyngeal Candida biofilm infection model,LIQ-TPA-TZ significantly eliminates infection,alleviates inflammation,and accelerates mucosal defect healing.This study provides a favorable strategy for confronting drug resistance,which may be a potential Candidate for the treatment of Candida infection. 展开更多
关键词 aggregation-induced emission biofilm candida infection mitochondrial targeting photosensitizer photodynamics therapy
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Temporal dynamics of bacterial biofilms and their relationship with heavy metals on expanded polystyrene microplastics 认领 引用 被引量：1
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作者 Qun XIE Yan FENG +7 位作者 Xiangrong XU Hengxiang LI Lang LIN Weiqiong YU Guanghui ZHU Nenghao ZHONG Minhua WU Kai ZHANG 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2025年第2期528-544,共17页
Expanded polystyrene (EPS) is a common type of microplastics (MPs) often found in coastal areas especially aquaculture areas.It is considered as an important site for microbial colonization and biofilm formation,as we... Expanded polystyrene (EPS) is a common type of microplastics (MPs) often found in coastal areas especially aquaculture areas.It is considered as an important site for microbial colonization and biofilm formation,as well as a carrier of pollutants like heavy metals.However,the dynamic changes of bacterial communities attached to EPS and their interaction with heavy metals are still poorly unknown.In this study,a one-year field exposure experiment was conducted at an aquaculture farm near Donghai Island,in Leizhou Bay,Zhanjiang,Guangdong,in South China Sea.The bacterial communities attached to EPS MPs were examined by 16S r DNA high-throughput sequencing,and the relationships between bacterial biofilms and heavy metals were explored.The results show that there were notable seasonal variations in the bacterial diversity of EPS MPs.Species biodiversity was the highest in summer and the lowest in winter.The greatest number of bacterial species and lowest level of uniformity were observed in the spring.The bacterial community structure changed with exposure time,and the most significant difference in the 12-month group (P<0.05) was found.The dominant bacterial species attached to EPS MPs were mainly Proteobackteria and Firmicutes at the phylum level,and Pseudomonas and Exiguobacterium were dominant at the genus level.Furthermore,EPS MPs acted as transport carriers for potential pathogenic bacteria.High correlations were found between bacterial species and the total concentration of heavy metals on EPS MPs,as well as their speciation fractions.Different chemical speciation of heavy metals migrated and altered over seasons within biofilms,which would further exacerbate the ecological risks. 展开更多
关键词 microplastic(MP) bacterial community biofilm heavy metal speciation fraction
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Mixotrophic Chlorella pyrenoidosa biofilm with enhanced biomass production,microalgal activity,and nutrient removal from nutrient-rich wastewater 认领 引用 被引量：1
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作者 Xiongwei Wu Cong Jin +5 位作者 Chiqian Zhang Ping Li Jim Junhui Huang Jing Wu Jinhua Wu Zhiqiang Hu 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2025年第11期366-377,共12页
Microalgae possess significant advantages in nitrogen and phosphorus removal from nutrient-richwastewater that are highly efficient and independent of the C/N ratio.However,challenges such as low biomass productivity,... Microalgae possess significant advantages in nitrogen and phosphorus removal from nutrient-richwastewater that are highly efficient and independent of the C/N ratio.However,challenges such as low biomass productivity,high variability in nutrient removal under different trophic types,and difficulty in harvesting biomass limits the large-scale application of microalgae wastewater treatment.This study attempted to employmixotrophic microalgae biofilm to address these issues.The biomass production,microalgal activity,and nutrient removal of Chlorella pyrenoidosa biofilms with different trophic types were compared for nutrient-rich wastewater treatment.The results showed that the biomass productivity of the mixotrophic microalgal biofilm(0.215 g/(L·d))was 2.3,8.6,and 6.0 times higher than that of photoautotrophic microalgal biofilm,heterotrophic microalgal biofilm,and photoautotrophic suspended microalga,respectively.Additionally,the dehydrogenase activity(DHA),indicating microalgal activity,of the mixotrophic biofilm was 2.3 and 16.5 times higher than that of photoautotrophic and heterotrophic biofilms,respectively.Meanwhile,the mixotrophic biofilm removed 96.0%of NH4+-N and 99.2%of PO43--P,more efficient than that with other types of biofilms and suspended microalgae.In an open-ended air-lift photobioreactor,the mixotrophic microalgal biofilm produced biomass at 0.12 g/(L·d)and removed 90.0%of NH4+-N and 97.6%of PO43--P.This study suggests that the mixotrophic microalgal biofilm shows promise in treating nutrient-rich wastewater and producing microalgal biomass for value-added products. 展开更多
关键词 Mixotrophic microalgae Algal biofilm Photobioreactor Nitrogen Phosphorus
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