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Heat stress induced testicular impairment is related to orchitis and complement activation in Rongchang boars 认领 引用
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作者 Xiangyuan Ma Wenxue Shen +11 位作者 Junhao Ni Xihao Luo Lianqiang Che Bin Feng Lun Hua Yong Zhuo Zhengfeng Fang Shengyu Xu Jian Li Xuemei Jiang Yan Lin De Wu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第1期488-499,共12页
Background Heat stress(HS)is posing as a tremendous threat to the swine industry,due to the thermos-sensitive gonads of boars.Testes are immune-privileged organs in which spermatogenesis needs to remain undisturbed,wh... Background Heat stress(HS)is posing as a tremendous threat to the swine industry,due to the thermos-sensitive gonads of boars.Testes are immune-privileged organs in which spermatogenesis needs to remain undisturbed,whereas immune cells are thermo-sensitive,especially macrophages,which are the most abundant testicular immune cells.Our study aimed to unveil the underlying immune responses and assess their consequences on the semen quality of boars under HS.The results will aid in addressing environmental temperature-related seasonal infertility and in selecting the best boar for use in artificial insemination.Methods The 3-week experiment assigned 268-week-old Rongchang male pigs into thermal neutral pair-feed(TN-PF)and HS groups.During the last 2 weeks,which served as the HS period,the HS group was subjected to 14-day 35±1℃,while the TN-PF group was kept at 26±1℃.Pig gonad tissues were sampled at the end of HS period for assessments and measurements.Results Our findings confirmed HS-related reactions such as elevated respiration rate(P<0.05)and elevated heat shock protein 60(HSP60;P<0.05)and heat shock protein 90(HSP90;P<0.05)expression levels.Sperm motility(P=0.06)and progressive sperms(P=0.08)were decreased under HS as was a significant reduction in average straight-line velocity(VSL;P<0.05).Additionally,total abnormality levels increased(P<0.05).Fibrosis,caspase-3 expression,and accumulations of tumor necrosis factor-α(TNF-α;P<0.05)and interleukin-1β(IL-1β;P<0.05),along with an elevated macrophage composition(P<0.05)characterized the orchitis under HS.Single cell RNA sequencing(scRNA-seq)revealed fluctuations in engulfing and inflammatory signals in testicular macrophages(TMs).In particular,the complement cascade was promoted by CD163+macrophages,resulting in membrane attack complex(C5b-9)assembly(P<0.05).Linear regressions further revealed a negative correlation between C5b-9 and sperm motility(P<0.05),as well as near-negative correlations between the C5b-9 and both progressive motility(P=0.08)and VSL(P=0.06).Conclusions Our findings highlighted the relationship between HS,the onset of orchitis,and the activation of the complement system,all of which decreased the boar semen quality. 展开更多
关键词 Boar Complement Heat stress Macrophage Orchitis Semen quality Testis
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Dynamic single-nucleus transcriptomic profiling of kidney development in Bamaxiang pigs and wild boar 认领 引用
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作者 Tian-Xiong Yao Yang Zhang +3 位作者 Xiao-Yun Chen Ting Luo Yang Li Si-Yu Yang 《Zoological Research》 SCIE CSCD 2026年第1期58-71,共14页
Defining developmental periods-and breed-specific cellular features of the porcine kidney is critical for refining donor selection strategies in xenotransplantation.This study utilized single-nucleus RNA sequencing to... Defining developmental periods-and breed-specific cellular features of the porcine kidney is critical for refining donor selection strategies in xenotransplantation.This study utilized single-nucleus RNA sequencing to generate 71081 high-quality transcriptomes from eight kidneys sampled across three developmental periods—weaning,fattening,and maturity—in Bamaxiang(BMX)and wild boar(WB)breeds.A total of 33 distinct cell types were identified.Proximal tubule(PT),principal(PC),and thick ascending limb(TAL)cells exhibited pronounced transcriptional transitions across developmental periods.PT cells displayed a temporal shift from early development-related functions during the weaning period to metabolic and transport functions during fattening and maturity.Subclustering of PT cells revealed discrete functional subtypes,including a mature WB-specific PT2 cluster enriched in immune function regulation.In PC cells,both breeds expressed a shared PC0 subtype during weaning with signatures of cellular development.The WBspecific PC2 subtype was enriched in thermoregulation pathways,whereas at maturity,the BMX-specific PC0 subtype exhibited transcriptional features associated with oxidative metabolism and the WB-specific PC1 subtype expressed genes involved in glucose metabolism.These findings suggest divergent physiological adaptations to domestication and wild environments.Cell-cell interaction analysis identified the epidermal growth factor(EGF)signaling pathway as the most active across BMX kidney development,with epithelial tubule subtypes engaging in ligand-receptor interactions via ligands,including betacellulin(BTC),EGF,and transforming growth factor-α(TGF-α). 展开更多
关键词 Pig kidney Bamaxiang pig Wild Boar Developmental period Single-nucleus RNA sequencing
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Geographical distribution and characterization of Jingmen tick virus in wild boars in China 认领 引用 被引量：1
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作者 Tong Wang Yu Guo +6 位作者 Yu Xu Heting Sun Peng Peng Siyuan Qin Guoqiang Zhu Changchun Tu Zhongzhong Tu 《Virologica Sinica》 SCIE CAS CSCD 2025年第1期137-140,共4页
Dear Editor,In recent decades,vector-transmitted emerging and re-emerging diseases pose public health issues around the world,in which emerging tick-borne viruses(TBVs)have played a major role since they are widely di... Dear Editor,In recent decades,vector-transmitted emerging and re-emerging diseases pose public health issues around the world,in which emerging tick-borne viruses(TBVs)have played a major role since they are widely distributed.TBVs have a wide range of hosts,including humans,livestock and rodents,with some of them able to cause severe diseases in human and domestic animals,such as Jingmen tick virus(JMTV)(Qin et al.,2014),tick-borne encephalitis virus(TBEV)(Xing et al.,2017),and Alongshan virus(ALSV)(Wang et al.,2019).Of the merging TBVs,JMTV is a novel pathogen that was first identified in Rhipicephalus microplus collected from the Jingmen city of Hubei province,China in 2010(Qin et al.,2014). 展开更多
关键词 Wild boars alongshan virus alsv wang Host range Jingmen tick virus China Public health issues Emerging tick borne viruses Geographical distribution
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Integrative pigGTEx resource with GWAS reveals genetic mechanism underlying semen quality in boars 认领 引用
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作者 Xuehua Li Qing Lin +7 位作者 Zhanwei Zhuang Kai Rao Zhili Li Xiuguo Shang Panjie Xia Lin Zhu Zhe Zhang Yunxiang Zhao 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第5期2040-2053,共14页
Background Semen quality is one of the most important indicators of boar reproductive performance.In the past,boar breeding has mostly emphasized characteristics such as lean meat percentage,feed conversion efficiency... Background Semen quality is one of the most important indicators of boar reproductive performance.In the past,boar breeding has mostly emphasized characteristics such as lean meat percentage,feed conversion efficiency,and growth rate,while overlooking the genetic improvement of reproductive traits.This study employs advanced multi-omics approaches,such as transcriptome-wide association studies(TWAS)and colocalization between genome-wide association studies(GWAS)and expression quantitative trait loci(eQTLs),to provide a comprehensive understanding of the genetic mechanisms governing semen quality traits in boars.Results Here,we collected 190,000 ejaculate records across 11 semen quality traits from 3,604 Duroc boars.The heritability of semen quality traits ranged from 0.095 to 0.343.Genetic correlations between semen quality traits varied from−0.802 to 0.661,and phenotypic correlations ranged from−0.833 to 0.776.Single-trait GWAS identified 19 independent variants,corresponding to 13 quantitative trait loci(QTLs).By integrating PigGTEx and FAANG resources,we combined TWAS and colocalization analyses to reveal genetic regulation of semen quality traits.Notably,both GWAS and colocalization analyses pinpointed the DCAF12 as a crucial gene associated with multiple semen quality traits.Additionally,the ZSCAN9 gene and the variant rs322211455 were found to significantly affect sperm motility(SPMOT),possibly through hypothalamic-pituitary-gonadal axis.PheWAS further highlighted an association between rs322211455 and sperm abnormality rate,demonstrating the crucial role of ZSCAN9 in male fertility.Conclusion This study reveals the genetic basis and regulatory mechanisms underlying semen quality traits in Duroc boars,identifying key candidate genes such as DCAF12 and ZSCAN9.These findings provide important insight into the genetic regulation of semen quality in boars. 展开更多
关键词 Boar Colocalization GWAS Semen quality TWAS
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Identification and genetic analysis of new ephemeroviruses in wild boars in China 认领 引用
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作者 Zhongzhong Tu Tong Wang +4 位作者 Yu Xu Heting Sun Peng Peng Siyuan Qin Changchun Tu 《Virologica Sinica》 SCIE CAS CSCD 2025年第2期186-191,共6页
Ephemeroviruses(EVs)are arthropod-borne rhabdoviruses and were isolated exclusively from cattle and haematophagous arthropods until two new ephemeroviruses were first identified from domestic pigs most recently.Here w... Ephemeroviruses(EVs)are arthropod-borne rhabdoviruses and were isolated exclusively from cattle and haematophagous arthropods until two new ephemeroviruses were first identified from domestic pigs most recently.Here we report the identification of newer EVs in wild boar by meta-transcriptomic(MTT)sequencing.Further screening by specific RT-nPCR of tissue samples of 459 free-ranging wild boars collected between 2018 and 2023 from 26 provinces across China confirmed five positive wild boars in four provinces.Interestingly,two ticks especially collected from two positive wild boars were also EV positive.Finally,four complete genome sequences of wild boar ephemeroviruses(WbEVs)were obtained with two strains belonging to a new EV species,and the rest two falling into porcine ephemerovirus 2(PoEV2)species identified from domestic pigs.Our study has further extended EV host range and demonstrated natural circulations of divergent EVs in wild boars,in which ticks may play roles.Biological implications of EV infection in wild boars should be interesting topics for future investigations. 展开更多
关键词 Wild boar Ephemerovirus(EVs) Tick China Genetic diversity
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Dietary n-6:n-3 ratio and Vitamin E improve motility characteristics in association with membrane properties of boar spermatozoa 认领 引用 被引量：11
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作者 Qing Liu Yuan-Fei Zhou +3 位作者 Run-Jia Duan Hong-Kui Wei Jian Peng Si-Wen jiang 《Asian Journal of Andrology》 SCIE CAS CSCD 2017年第2期223-229,共7页
This study was aimed to evaluate the effects of dietary n-6：n-3 ratio and Vitamin E on the membrane properties and motility characteristics of spermatozoa in boars. Forty Duroc boars were randomly distributed in a 2 ... This study was aimed to evaluate the effects of dietary n-6：n-3 ratio and Vitamin E on the membrane properties and motility characteristics of spermatozoa in boars. Forty Duroc boars were randomly distributed in a 2 × 2 factorial design with two n-6：n-3 ratios （14.4 and 6.6） and two Vitamin E levels （200 and 400 mg kg-1）. During 16 weeks of treatment, fresh semen was collected at weeks O, 8, 12, and 16 for measurements of motility characteristics, contents of fatty acids, membrane properties （membrane fluidity and membrane integrity）, and lipid peroxidation of the spermatozoa. The semen was diluted in Beltsville Thawing Solution （BTS） extender and stored at 17℃, and the sperm motility was assessed at 12, 36, 72, and 120 h of storage. The 6.6 n-6.n-3 dietary ratio increased the contents of n-3 polyunsaturated fatty acids （PUFAs） and docosabexaenoic acid （DHA） and improved the membrane integrity and membrane fluidity of the spermatozoa, resulting in notably increased total motility, sperm progressive motility, and velocity parameters of fresh semen. Feeding diet with Vitamin E （400 mg kg-1） prevented sperm lipid peroxidation, and resulted in higher total motility and sperm progressive motility in fresh and liquid stored semen. In conclusion, the adjustment of n-6.n-3 ratio （6.6） and supply of Vitamin E （400 mg kg-1） successfully improved sperm motility characteristics and thus may be beneficial to the fertility of boars, which might be due to the modification of the physical and functional properties of spermatozoa membrane in response to dietary supplementation. 展开更多
关键词 boar dietary n-6：n-3 ratio fatty acid composition lipid peroxidation membrane properties motility characteristics Vitamin E
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Conflict between wild boars(Sus scrofa) and farmers:distribution, impacts,and suggestions for management of wild boars in the Three Gorges Reservoir Area 认领 引用 被引量：7
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作者 LIU Qin YAN Kun +2 位作者 LU Ya-feng LI Ming YAN Yang-yang 《Journal of Mountain Science》 SCIE CSCD 2019年第10期2404-2416,共13页
Economic losses caused by wild boars(Sus scrofa)continue to increase in the Three Gorges Reservoir Area.We selected 206 farmers in Wuqiaohe Basin as survey respondents using random stratified sampling.The distribution... Economic losses caused by wild boars(Sus scrofa)continue to increase in the Three Gorges Reservoir Area.We selected 206 farmers in Wuqiaohe Basin as survey respondents using random stratified sampling.The distribution and impacts of wild boars were examined using a questionnaire survey and spatial analysis.The results showed that:(1)The density of farmer settlements is low and most peasants’households are located on the forest edge.Due to large-scale labor migration,a high proportion of farmland(25.85%)has been abandoned and an even higher proportion of farmland(40.48%)has been returned to forest.(2)The probability of wild boar damage to peasant households was 67.96%.Yields of corn and sweet potato decreased by 24.87%and 28.24%,respectively,with a total economic loss of approximately 2,590,100 RMB/yr in the Wuqiaohe basin.Sixty-five percent of the affected cultivatedlands were located within approximately 150 m of the forest edges.(3)The wild boar damage coefficient was significantly and negatively correlated with the distance between the peasants’household and the forest edge,the density of the settlements,and the area of cultivated land.The wild boar damage coefficient shows a significant positive correlation with the area of farmland returned to forest.(4)The damage activity caused by wild boars began to occur7-8 years ago,and the destruction has been more frequently and seriously observed in the last 4-5 years.(5)We make suggestions for wild boar control and management measures including developing a compensation policy.The compensation standard is related to the land area returning from farmland to forests and the actual cultivated land area. 展开更多
关键词 Distribution features Economic loss Management Three Gorges Reservoir Area Wild boar
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Epizootiological surveillance of porcine circoviruses in free-ranging wild boars in China 认领 引用 被引量：5
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作者 Wenjie Gong Haiying Du +9 位作者 Tong Wang Heting Sun Peng Peng Siyuan Qin Haidong Geng Zheng Zeng Wangwang Liang Hongquan Ling Changchun Tu Zhongzhong Tu 《Virologica Sinica》 SCIE CAS CSCD 2023年第5期663-670,共8页
Four species of porcine circoviruses(PCV1–4)have been reported to circulate in Chinese domestic pigs,while the epizootiology of these viruses in free-ranging wild boars in China remains unknown.In this study,tissue a... Four species of porcine circoviruses(PCV1–4)have been reported to circulate in Chinese domestic pigs,while the epizootiology of these viruses in free-ranging wild boars in China remains unknown.In this study,tissue and serum samples collected from diseased or apparently healthy wild boars between 2018 and 2020 in 19 regions of China were tested for the prevalence of PCV1–4 infections.Positive rates of PCV1,PCV2,and PCV3 DNA in the tissue samples of Chinese wild boars were 1.6%(4/247),58.3%(144/247),and 10.9%(27/247)respectively,with none positive for PCV4.Sequence analysis of viral genome showed that the four PCV1 strains distributed in Hunan and Inner Mongolia shared 97.5%–99.6%sequence identity with global distributed reference strains.Comparison of the ORF2 gene sequences showed that 80 PCV2 strains widely distributed in 18 regions shared 79.5%–100%sequence identity with reference strains from domestic pigs and wild boars,and were grouped into PCV2a(7),PCV2b(31)and PCV2d(42).For PCV3,17 sequenced strains shared 97.2%–100%nucleotide identity at the genomic level and could be divided into PCV3a(3),PCV3b(2)and PCV3c(12)based on the phylogeny of ORF2 gene sequences.Serological data revealed antibody positive rates against PCV1 and PCV2 of 11.4%(19/167)and 53.9%(90/167)respectively.The data obtained in this study improved our understanding about the epidemiological situations of PCVs infection in free-ranging wild boars in China and will be valuable for the prevention and control of diseases caused by PCVs infection. 展开更多
关键词 Porcine circoviruses(PCVs) Epizootiology Wild boar China
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=actors influencing wild boar damage to agricultural crops in Sardinia (Italy) 认领 引用 被引量：4
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作者 Marco LOMBARDINI Alberto MERIGGI Alberto FOZZL 《Current Zoology》 SCIE CAS CSCD 2017年第5期507-514,共8页
Crop damage by wildlife is a frequent source of human-wildlife conflict. Understanding which fac-tors increase the risk of damage is crucial to the development of effective management strategies.The aims of this study... Crop damage by wildlife is a frequent source of human-wildlife conflict. Understanding which fac-tors increase the risk of damage is crucial to the development of effective management strategies.The aims of this study were to provide a general description of agricultural damage caused by wildboar Sus scrofa meridionalis over a 7-year period in North-eastern Sardinia （Mediterranean Italy）,and to formulate a predictive model of damage risk. We recorded a total of 221 cases of wild boardamage, with economic losses amounting to 483,982 Euros. Damage events mostly involved vine-yards, meadows and oat fields, and were characterized by a peak incidence in summer and earlyautumn, and a minimum in spring. Damaged fields were characterized by an increasing presenceof permanent crops, a decreasing presence of woodlands, maquis and urban areas, and a reduceddistance from shelter areas （forests and shrublands）. The analysis of spatiotemporal variation ofboar-induced damage and the identification of factors that augment the risk of damage providesessential information for contributing to the development of a more effective plan for managingwild boar populations. 展开更多
关键词 Human-wildlife conflict, Sus scrofa meridionalis, risk modelling, landscape structure, wild boar management.
Patterns of alteration in boar semen quality from 9 to 37 months old and improvement by protocatechuic acid 认领 引用 被引量：2
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作者 Ruizhi Hu Xizi Yang +10 位作者 Jiatai Gong Jing Lv Xupeng Yuan Mingkun Shi Chenxing Fu Bie Tan Zhiyong Fan Liang Chen Hongfu Zhang Jianhua He Shusong Wu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第5期1881-1893,共13页
Background Comprehending the patterns of alteration in boar semen quality and identifying effective nutritional interventions are crucial for enhancing the productivity of commercial pig systems.This study aimed to ex... Background Comprehending the patterns of alteration in boar semen quality and identifying effective nutritional interventions are crucial for enhancing the productivity of commercial pig systems.This study aimed to examine the alteration in semen quality in boars,and assess the impact of protocatechuic acid(PCA)on semen quality during the phase of declining semen quality.Methods In Exp.1,a total of 38 Pig Improvement Company(PIC)boars were selected and their semen quality data were recorded from the age of 9 to 37 months.In Exp.2,18 PIC boars(28 months old)were randomly assigned into three groups(n=6)and fed a basal diet,a basal diet containing 500 or 1,000 mg/kg PCA,respectively.The experiment lasted for 12 weeks.Results The semen volume,concentration,and total number of spermatozoa in boars exhibited an increase from 9 to 19 months old and showed a significant linear decreased trend in 28,24,and 22 months old.Sperm motility displayed an upward trajectory,reaching its peak at 20 months of age,and showed a significant linear decreased trend at 20 months old.Dietary supplementation of PCA demonstrated an effect to mitigate the decrease in semen volume,concentration of spermatozoa,total number of spermatozoa(P>0.05),and significantly increased the sperm motility(P<0.05).Moreover,supplementation of 1,000 mg/kg PCA significantly increased the sperm viability(P<0.05).Analysis on cellular signaling pathways revealed that PCA restored serum testosterone levels and alleviated oxidative damage by upregulating the expression of HO-1,SOD2,and NQO1 in testicular stromal cells.Notably,PCA can enhance phosphorylation by selectively binding to AMP-activated protein kinase(AMPK)protein,thereby improving sperm mitochondrial function and augmenting sperm motility via PGC-1/Nrf1.Conclusions These data elucidated the pattern of semen quality variation in boars within the age range of 9 to 37 months old,and PCA has the potential to be a natural antioxidant to enhance sperm quality through modulation of the AMPK/PGC-1/Nrf1 signaling pathway. 展开更多
关键词 Antioxidants Boars Protocatechuic acid Semen quality Signaling pathway
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Source and level of dietary iron influence semen quality by affecting inflammation, oxidative stress and iron utilization levels in boars 认领 引用 被引量：2
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作者 Yinghui Wu Yamei Li +8 位作者 Yueyue Miao Hongkui Wei Hefeng Luo Chunxiao Ren Yawei Zhang Juan Chen Tanghong Wei Jiyan Deng Jian Peng 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第5期2044-2058,共15页
Background Boars fed a mixed form of inorganic and organic iron in excess of the NRC recommended levels still develop anemia,which suggested that the current level and form of iron supplementation in boar diets may be... Background Boars fed a mixed form of inorganic and organic iron in excess of the NRC recommended levels still develop anemia,which suggested that the current level and form of iron supplementation in boar diets may be inappropriate.Therefore,56 healthy Topeka E line boars aged 15–21 months were randomly divided into 5 groups:basal diet supplemented with 96 mg/kg ferrous sulfate(FeSO4)and 54 mg/kg glycine chelated iron(Gly-Fe,control);80 mg/kg or 115 mg/kg Gly-Fe;80 mg/kg or 115 mg/kg methionine hydroxyl analogue chelated iron(MHA-Fe,from CalimetFe)for 16 weeks.The effects of dietary iron supplementation with different sources and levels on semen quality in boars were investigated.Results 1)Serum Fe and hemoglobin concentrations were not affected by reduced dietary iron levels in the 80 mg/kg or 115 mg/kg Gly-Fe and MHA-Fe groups compared with the control group(P>0.05).2)Serum interleukin-6(IL-6)and sperm malondialdehyde(MDA)levels in the 80 mg/kg or 115 mg/kg MHA-Fe groups were lower than those in the control group(P<0.05),and higher serum superoxide dismutase levels and lower MDA levels in the 115 mg/kg MHA-Fe group(P<0.05).3)Boars in the 80 mg/kg or 115 mg/kg Gly-Fe and MHA-Fe groups had lower serum hepcidin(P<0.01),ferritin(P<0.05),and transferrin receptor(P<0.01)concentrations,and boars in the 115 mg/kg MHA-Fe group had higher seminal plasma Fe concentrations compared with the control group.4)Boars in the 80 mg/kg and 115 mg/kg MHA-Fe groups had lower abnormal sperm rate and in situ oscillating sperm ratio compared to the control group at weeks 12 and/or 16 of the trial.However,the effect of Gly-Fe on improving semen quality in boars was not evident.5)Serum IL-6 level was positively correlated with hepcidin concentration(P<0.05),which in turn was significantly positively correlated with abnormal sperm rate(P<0.05).Furthermore,significant correlations were also found between indicators of iron status and oxidative stress and semen quality parameters.Conclusions Dietary supplementation with 80 mg/kg or 115 mg/kg MHA-Fe did not induce iron deficiency,but rather reduced serum inflammatory levels and hepcidin concentration,alleviated oxidative stress,increased body iron utilization,and improved semen quality in adult boars. 展开更多
关键词 Adult boars Iron level Iron source Iron status Semen quality
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Effects of pituitary-specific overexpression of FSHα/β on reproductive traits in transgenic boars 认领 引用 被引量：1
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作者 Wenting Li Yujun Quan +5 位作者 Mengmeng Zhang Kejun Wang Muzhen Zhu Ye Chen Qiuyan Li Keliang Wu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2018年第1期51-58,共8页
Background： Follicle-stimulating hormone（FSH） is a gonadotropin synthesized and secreted by the pituitary gland.FSH stimulates follicle development and maturation in females. It also plays an important role in sper... Background： Follicle-stimulating hormone（FSH） is a gonadotropin synthesized and secreted by the pituitary gland.FSH stimulates follicle development and maturation in females. It also plays an important role in spermatogenesis in males, including humans and mice. However, the effects of FSH on male pigs are largely unknown. In this study,we generated transgenic pigs to investigate the effects of FSHα/β overexpression on reproductive traits in boars.Results： After five transgenic F0 founders were crossed with wide-type pigs, 193 F_1 animals were obtained. Of these,96 were confirmed as transgenic. FSHα and FSHβ m RNAs were detected only in pituitary tissue. Transgenic boars exhibited significantly higher levels of FSHα and FSHβ m RNA, serum FSH, and serum testosterone, compared to ful-sib non-transgenic boars. Significant increases in testis weight, vas deferens diameter, seminiferous tubule diameter,and the number of Leydig cel s were observed, suggesting that the exogenous FSHα/β affects reproductive traits. Final y,transgenic and non-transgenic boars had similar growth performance and biochemical profiles.Conclusions： Pituitary-specific overexpression of FSHα/β genes is likely to impact reproductive traits positively, as indicated by enhancements in serum testosterone level, testis weight, the development of vas deferens, seminiferous tubules, and Leydig cells in transgenic boars. A high level of serum FSH induces secretion of serum testosterone,possibly by boosting the number of Leydig cells, which presumably increases the libido and the frequency of sexual activity in transgenic boars. Our study provides a preliminary foundation for the genetic improvement of reproductive traits in male pigs. 展开更多
关键词 Boar FSHα/β Reproductive traits Transgene
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Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China 认领 引用 被引量：1
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作者 GUO Huan-cheng REN Zhao-wen +10 位作者 DING Mei-ming XIAO Wan-jun PENG Peng HE Biao FENG Ye LIU Yan LI Xing-yu CAI Jian-qiu ZHANG Bi-kai LUO Qing-hua TU Chang-chun 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2019年第9期2133-2140,共8页
Hybrid wild boar husbandry is an important component of livestock production in Northeast China.However,the current disease situation of these animals is largely unknown due to a lack of disease surveillance.The prese... Hybrid wild boar husbandry is an important component of livestock production in Northeast China.However,the current disease situation of these animals is largely unknown due to a lack of disease surveillance.The present study was conducted to determine the prevalence of several important viral diseases in the hybrid wild boar population of Northeast China.Between September 2015 to December 2016,169 blood and 61 tissue samples were collected from apparently healthy hybrid wild boars from farms in Jilin,Inner Mongolia and Heilongjiang provinces.ELISA detected serum antibodies against classical swine fever virus(CSFV),porcine reproductive and respiratory syndrome virus(PRRSV),pseudorabies virus(PRV),porcine circovirus type 2(PCV2)and Japanese encephalitis virus(JEV),but not against African swine fever virus(ASFV),with PCV2 having the highest seropositive rate(87.2–100%in different farms).RT-PCR or PCR performed on the processed samples detected only PCV2,with 33.1%(56/169)of blood samples and 32.8%(20/61)of spleen samples being positive,respectively,indicating widespread PCV2 infection in hybrid wild boars.Phylogenetic analysis of 15 PCV2 ORF2 sequences showed that they belong to genotypes PCV2a,PCV2b and PCV2d,with nucleotide and deduced amino acid homologies of 88.5–100%and 88.1–100%,respectively. 展开更多
关键词 disease transmission hybrid wild boars Northeast China serological and molecular survey viral diseases
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Snapshot of Structural Variations in the Tibetan Wild Boar Genome at Single-Nucleotide Resolution 认领 引用 被引量：1
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作者 Lei Chen Long Jin +9 位作者 Mingzhou Li Shilin Tian Tiandong Che Qianzi Tang Jing Lan Zhi Jiang Ruiqiang Li Yiren Gu Xuewei Li Jinyong Wang 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2014年第12期653-657,共5页
Genomic structural variations （SVs）, particularly insertions, deletions and inversions, can contribute to the heterogeneity of millions of nucleotides within a genome, and are likely to make an important contributio... Genomic structural variations （SVs）, particularly insertions, deletions and inversions, can contribute to the heterogeneity of millions of nucleotides within a genome, and are likely to make an important contribution to biological diversity and phenotypic variation （Alkan et al., 2011; Bickhart and Liu, 2014）. With the rapid development of the next-generation sequencing technologies and the new assembly methodolo- gies, the multiple de novo assemblies of genomes within a species allow researchers to explore more detailed SV maps （Li et al., 2011）. Compared with the traditional read depth algorithm using the whole-genome resequencing approach and array-based technologies （Baker, 2012; Wang et al., 2012; 展开更多
关键词 gene Snapshot of Structural Variations in the Tibetan Wild Boar Genome at Single-Nucleotide Resolution
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Bacteriospermia among smallholder artificial insemination boars in the Philippines and potential associated factors 认领 引用
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作者 Santiago T.Peña Jr. Ma.Delia A.Pagente +1 位作者 Bianca Therese P.Ymas Mark Edd B.Janier 《Asian pacific Journal of Reproduction》 CAS 2023年第1期35-41,共7页
Objective:To determine the prevalence of bacteriospermia,the bacterial load,and the potential factors associated with bacterial contamination in boar semen collected by local smallholder artificial insemination operat... Objective:To determine the prevalence of bacteriospermia,the bacterial load,and the potential factors associated with bacterial contamination in boar semen collected by local smallholder artificial insemination operators.Methods:Fifteen individual raw semen samples were collected from locally available artificial insemination boars owned by different smallholder boar operators within the 5th district of Leyte,Philippines and were subjected to standard bacteriological culture and identification,including a survey of potentially associated factors.Prevalence and bacterial count were determined accordingly,while boar characteristics and collection practices were clustered following agglomerative hierarchical clustering technique.Results:One hundred percent contamination with a bacterial count of(2.01±0.38)×103 CFU/mL was observed.At least 73.33%of the samples were positive for Bacillus spp.,while other identified isolates included Enterobacter spp.,Staphylococcus spp.,E.coli,Pseudomonas spp.,Citrobacter spp.,and Klebsiella spp.Conclusions:Despite the high prevalence of bacteriospermia,the bacterial count is low.Nevertheless,on-farm practices on boar health and management,semen collection,and sanitation as well as the enhancement of basic protocols to control contamination should be conscientiously considered in smallholder artificial insemination operation. 展开更多
关键词 Bacteriospermia Local artificial insemination boars Boar sperm Artificial insemination Semen quality Smallholder pigs
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Investigation on the Harm of Wild Boar in Mingtong Town, Chengkou, Chongqing 认领 引用 被引量：1
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作者 王正春 张后伟 吴旭 《Agricultural Science & Technology》 CAS 2017年第12期2446-2449,共4页
Through the investigation and analysis of the wild boars in Mingtong Town, Chengkou, Chongqing, it was concluded that the area had long been seri- ously affected by wild boars and some of the human activities were pot... Through the investigation and analysis of the wild boars in Mingtong Town, Chengkou, Chongqing, it was concluded that the area had long been seri- ously affected by wild boars and some of the human activities were potentially threatened, and the potential threat areas were identified. Corresponding suggestions were proposed to guide the prevention and control work, so as to protect the habi- tat and reproduction of wildlife and ensure the normal production and life of the masses. 展开更多
关键词 Wild boar Harm Threat Prevention and control
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Effects of Three Different Diluents on Quality of Boar Semen Stored at 17℃ 认领 引用
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作者 Hu Shan Zhang Xiao-gang +4 位作者 Han Cong Wei Shuai-yi Xie Dong-qi Du Ren-rang Hu Jian-hong 《Journal of Northeast Agricultural University(English Edition)》 CAS 2015年第2期36-46,共11页
To investigate the effects of different diluents on the quality of the boar semen stored at 17℃, and assess the relationship between sperm motility and the relative levels of enzymes, three commercial diluents （Dilu... To investigate the effects of different diluents on the quality of the boar semen stored at 17℃, and assess the relationship between sperm motility and the relative levels of enzymes, three commercial diluents （Diluent I, Diluent II and Diluent III） and three boar breed semens （Yorkshire, Landrace and Duroc） were utilized. The sperm motility, effective survival time, survival index, catalase （CAT）, the total anti-oxidative capacity （T-AOC） and malondialdehyde （MDA） levels were evaluated. The results showed that there were significant interaction effects between diluents and breeds on the boar sperm motility （P〈0.001）, survival time （P〈0.001）, CAT levels （P〈0.001） and T-AOC levels （P〈0.001）, but neither effects nor interaction effects between diluents and breeds on survival index （P〉0.05）. All of the parameters varied significantly with the increase of the storage time （P〈0.001）. The survival time increased 12.9% in Yorkshire boar semen diluted with Diluent III than with Diluent II, while the survival time increased 6.6% in Landrace boar semen diluted with Diluent II than with Diluent III. Both CAT and T-AOC levels were significantly positive correlated with sperm motility in all the three boar breeds （P〈0.001）, while MDA levels were significantly negative correlated with sperm motility （P〈0.001）. These results indicated that Diluent III and Diluent II were the optimal commercial diluents for Yorkshire and Landrace boar semen stored at 17 ℃, respectively. 展开更多
关键词 boar semen storage different diluents survival index enzyme activity
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GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm 认领 引用 被引量：8
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作者 Marc Llavanera Ariadna Delgado-Bermúdez +5 位作者 Beatriz Fernandez-Fuertes Sandra Recuero Yentel Mateo Sergi Bonet Isabel Barranco Marc Yeste 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2019年第4期931-941,共11页
Background: Cryopreservation is currently the most efficient method for long-term preservation of mammalian gametes and is extensively used in swine artificial insemination(AI) centres. However, it is well-known that ... Background: Cryopreservation is currently the most efficient method for long-term preservation of mammalian gametes and is extensively used in swine artificial insemination(AI) centres. However, it is well-known that cryopreservation procedures induce changes in the water phase in both intra and extracellular compartments,which alter the content and localisation of several proteins and ends up curtailing the structural integrity of functional sperm(i.e., cryoinjuries). Alterations and deficiencies of sperm-oocyte binding proteins during gamete recognition are one of the causes of reproductive failure both in vitro and in vivo. In this sense, characterisation of cryopreservation effects upon oocyte-binding proteins of sperm, such as IZUMO1 and GSTM3, is essential when assessing the impact of this technique in swine reproduction.Results: Cryopreservation was found to induce changes in the localisation of IZUMO1 and GSTM3 in boar sperm.However, the relative content of both proteins was not altered after thawing. Furthermore, whereas IZUMO1 content was found not to be related to the cryotolerance of boar sperm, GSTM3 content was observed to be higher in poor(PFE) than in good(GFE) freezability ejaculates in both pre-frozen(1.00 INT·mm^2± 0.14 INT·mm^2 vs.0.72 INT·mm^2± 0.15 INT·mm^2;P < 0.05) and post-thawed(0.96 INT·mm^2± 0.20 INT·mm^2 vs. 70 INT·mm^2± 0.19 INT·mm^2;P < 0.05) samples. Moreover, GSTM3 levels were found to be higher in those spermatozoa that exhibited low mitochondrial activity, high reactive oxygen species(ROS) production, and high membrane lipid disorder postthaw(P < 0.05).Conclusions: The difference in GSTM3 content between GFE and PFE, together with this protein having been found to be related to poor sperm quality post-thaw, suggests that it could be used as a cryotolerance marker of boar spermatozoa. Furthermore, both IZUMO1 and GSTM3 relocate during cryopreservation, which could contribute to the reduced fertilising capacity of frozen-thawed boar sperm. 展开更多
关键词 Boar Cryopreservation GSTM3 IZUMO1 ROS Sperm
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Gene expression changes in spleens of the wildlife reservoir species,Eurasian wild boar(Sus scrofa),naturally infected with Brucella suis biovar 2 认领 引用 被引量：8
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作者 Ruth C.Galindo Pilar M.Muoz +6 位作者 María J.de Miguel Clara M.Marin Javier Labairu Miguel Revilla José M.Blasco Christian Gortazar José de la Fuente 《Journal of Genetics and Genomics》 SCIE CAS 2010年第11期725-736,共12页
Brucella suis is responsible for swine brucellosis worldwide. Of the five different B. suis biovars （by.）, bv. 2 appears restricted to Europe where it is frequently isolated from wild boar and hares, can infect pigs... Brucella suis is responsible for swine brucellosis worldwide. Of the five different B. suis biovars （by.）, bv. 2 appears restricted to Europe where it is frequently isolated from wild boar and hares, can infect pigs and can cause human brucellosis. In this study, the differ- ential gene expression profile was characterized in spleens of Eurasian wild boar naturally infected with B. suis bv. 2. Of the 20,201 genes analyzed in the microarray, 633 and 1,373 were significantly （fold change 〉 1.8; P 〈 0.01） upregulated and downregulated, respectively, in infected wild boar. The analysis was focused on genes that were over represented after conditional test for biological process gene ontology. Upregulated genes suggested that B. suis bv. 2 infection induced cell maturation, migration and/or proliferation in infected animals. The genes downregulated in infected wild boar impaired the activity of several important cellular metabolic pathways such as metabolism, cytoskeleton organization and biogenesis, immune response and lysosomal function and vesicle-mediated transport. In addition, the response to stress, sperm fertility, muscle development and apoptosis seemed to be also impaired in infected animals. These results suggested that B. suis bv. 2 may use strategies similar to other smooth brucellae to facilitate intracellular multiplication and the development of chronic infections. To our knowledge, this is the first report of the analysis of gene expression profile in hosts infected with B. suis bv. 2, which is important to understand the molecular mechanisms at the host-pathogen interface in the main reservoir species with possible implications in the zoonotic cycle of the pathogen. 展开更多
关键词 Brucella suis boar genomics microarray systems biology
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Genetics Research and Advance on Development and Utilization of Wild Boars 认领 引用
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作者 LIU Chunlong LIU Di LI Zhongqiu 《Journal of Northeast Agricultural University(English Edition)》 CAS 2011年第3期83-87,共5页
Wild boar is one of the most important beast resources. It plays an important role in the maintenance of biological diversity. The genetic resources of wild boar can not only protect the genetic resources, but also im... Wild boar is one of the most important beast resources. It plays an important role in the maintenance of biological diversity. The genetic resources of wild boar can not only protect the genetic resources, but also improve the formation of new breeds in pigs. This paper summarized the advance on the main biological characteristics of wild boars, evolutionary origin between wild boars and domesticated pigs, and development and utilization of wild boars aimed to provide further insight into wild boar＇s genetic research and its resource protection. 展开更多
关键词 wild boar biological characteristics genetics development and utilization
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