Crotalaria is a genus of the Fabaceae family with agricultural and medicinal value,but to date the genome has not been fully sequenced.Although Crotalaria pallida is widely distributed in tropical and subtropical regi...Crotalaria is a genus of the Fabaceae family with agricultural and medicinal value,but to date the genome has not been fully sequenced.Although Crotalaria pallida is widely distributed in tropical and subtropical regions,the degree of genetic diversity and the specific traits influenced by geographic dispersal remain unknown.We here report a high-quality genome assembly of C.pallida with 98.52%coverage which is assembled into 8 chromosomes.C.pallida is closely related to Lupinus angustifolius,with genetic divergence occurring∼42.5-57.4 million years ago(MYA).Re-sequencing of 236 C.pallida accessions revealed a genetic diversity decrease as C.pallida spread from Africa to America and Asia,and from Asia to China and finally to Hainan.Significant divergence was observed in seven traits between non-Hainan and Hainan accessions.Genome-wide association studies identified 73 loci for 18 agronomic traits,25 of which overlapped with divergent sweeps between non-Hainan accessions and Hainan accessions.Furthermore,the dispersal of C.pallida in Hainan reduced genetic diversity,leading to a divergence in allelic frequencies at four candidate genes(CpPTR,CpMYB,CpRLPK,and CpNADK)associated with plant height.This study reveals the genetic basis of trait divergence driven by geographic dispersal and offers valuable resources for the strategic development of C.pallida breeding.展开更多
The dramatic expansion of the human neocortex,roughly three times larger than our closest primate relatives,underlies our distinctive cognitive capabilities.Central to this expansion are upper-layer cortical neurons(l...The dramatic expansion of the human neocortex,roughly three times larger than our closest primate relatives,underlies our distinctive cognitive capabilities.Central to this expansion are upper-layer cortical neurons(layers 24),which form intricate cortico-cortical circuits underlying language,abstract reasoning,and complex social behavior[1].During cortical development,radial glial cells(RGCs)—the primary neural progenitor cells in the ventricular zone-transition from self-renewing divisions to asymmetric divisions that generate both neurogenic intermediate progenitors(IPs)and neurons.These IPs are capable of undergoing symmetric divisions,thereby contributing to cortical neuron production,and a majority of them are destined to become upper-layer neurons[2].展开更多
Decapod Iridescent Virus 1(DIV1)is a recently discovered virus recognized for its high infectivity in Macrobrachium rosenbergii.A thermal treatment was performed on DIV1-infected M.rosenbergii,and the therapeutic effi...Decapod Iridescent Virus 1(DIV1)is a recently discovered virus recognized for its high infectivity in Macrobrachium rosenbergii.A thermal treatment was performed on DIV1-infected M.rosenbergii,and the therapeutic efficacy was evaluated.In the DIV1 challenge experiment,the mortality rate in the challenged group was found to be 2.6 times greater than that in the control group,with the viral load in deceased individuals exceeding 5.41×107copies/μg-DNA.The thermal treatment(TT)was administered at 36℃for a duration of 16 d,followed by a temperature restoration(TR)period at 26℃for 3 d.On the first day at 36℃,an average viral concentration of 5.34×10 copies/μg-DNA was detected in the survived individuals.RNA-seq analysis showed a significant upregulation of genes related to the lysosome pathway,including sialin-like isoform x2(slc17a5),beta-galactosidase-1-like protein 2(glb1),putative glucosylceramidase 3(gba),sphingomyelin phosphodiesterase-like isoform x2(smpd1),betahexosaminidase subunit alpha-like(hexa_b)and legumain-like protein(lgmn),following a transient suppression period induced by thermal stress.Upon reaching 36℃,the activation of heat shock protein 70(hsp70)and heat shock protein 90(hsp90a)was observed.Concomitantly,genes that implicated in energy production critical for DIV1 replication,such as hexokinase(hk)and microsomal glutathione stransferase 3-like isoform x2(gst),were inhibited.These results collectively suggest that TT/TR treatments eliminated DIV1 in M.rosenbergii by activating the organism’s innate immune response and suppressing virus replication.This study provides a theoretical basis for utilizing thermal therapy in the management of viral infections in M.rosenbergii breeding programs,thereby facilitating the development of new strains resistant to DIV1.展开更多
基金supported by Chinese Academy of Tropical Agricultural Sciences for Science and Technology Innovation Team of National Tropical Agricultural Science Center(no.CATASCXTD202503)the China Agriculture Research System of MOF and MARA(no.CARS-34)the National Science and Technology Basic Resources Investigation(no.2017FY100600).
摘要Crotalaria is a genus of the Fabaceae family with agricultural and medicinal value,but to date the genome has not been fully sequenced.Although Crotalaria pallida is widely distributed in tropical and subtropical regions,the degree of genetic diversity and the specific traits influenced by geographic dispersal remain unknown.We here report a high-quality genome assembly of C.pallida with 98.52%coverage which is assembled into 8 chromosomes.C.pallida is closely related to Lupinus angustifolius,with genetic divergence occurring∼42.5-57.4 million years ago(MYA).Re-sequencing of 236 C.pallida accessions revealed a genetic diversity decrease as C.pallida spread from Africa to America and Asia,and from Asia to China and finally to Hainan.Significant divergence was observed in seven traits between non-Hainan and Hainan accessions.Genome-wide association studies identified 73 loci for 18 agronomic traits,25 of which overlapped with divergent sweeps between non-Hainan accessions and Hainan accessions.Furthermore,the dispersal of C.pallida in Hainan reduced genetic diversity,leading to a divergence in allelic frequencies at four candidate genes(CpPTR,CpMYB,CpRLPK,and CpNADK)associated with plant height.This study reveals the genetic basis of trait divergence driven by geographic dispersal and offers valuable resources for the strategic development of C.pallida breeding.
基金supported by the Scientific Research Foundation of Hunan Provincial Education Department(24C0234)the Natural Science Foundation of Hunan Province(2026JJ60392).
摘要The dramatic expansion of the human neocortex,roughly three times larger than our closest primate relatives,underlies our distinctive cognitive capabilities.Central to this expansion are upper-layer cortical neurons(layers 24),which form intricate cortico-cortical circuits underlying language,abstract reasoning,and complex social behavior[1].During cortical development,radial glial cells(RGCs)—the primary neural progenitor cells in the ventricular zone-transition from self-renewing divisions to asymmetric divisions that generate both neurogenic intermediate progenitors(IPs)and neurons.These IPs are capable of undergoing symmetric divisions,thereby contributing to cortical neuron production,and a majority of them are destined to become upper-layer neurons[2].
基金Supported by the earmarked fund for CARS(No.CARS-48)the National Natural Science Foundation of China(No.32202964)。
摘要Decapod Iridescent Virus 1(DIV1)is a recently discovered virus recognized for its high infectivity in Macrobrachium rosenbergii.A thermal treatment was performed on DIV1-infected M.rosenbergii,and the therapeutic efficacy was evaluated.In the DIV1 challenge experiment,the mortality rate in the challenged group was found to be 2.6 times greater than that in the control group,with the viral load in deceased individuals exceeding 5.41×107copies/μg-DNA.The thermal treatment(TT)was administered at 36℃for a duration of 16 d,followed by a temperature restoration(TR)period at 26℃for 3 d.On the first day at 36℃,an average viral concentration of 5.34×10 copies/μg-DNA was detected in the survived individuals.RNA-seq analysis showed a significant upregulation of genes related to the lysosome pathway,including sialin-like isoform x2(slc17a5),beta-galactosidase-1-like protein 2(glb1),putative glucosylceramidase 3(gba),sphingomyelin phosphodiesterase-like isoform x2(smpd1),betahexosaminidase subunit alpha-like(hexa_b)and legumain-like protein(lgmn),following a transient suppression period induced by thermal stress.Upon reaching 36℃,the activation of heat shock protein 70(hsp70)and heat shock protein 90(hsp90a)was observed.Concomitantly,genes that implicated in energy production critical for DIV1 replication,such as hexokinase(hk)and microsomal glutathione stransferase 3-like isoform x2(gst),were inhibited.These results collectively suggest that TT/TR treatments eliminated DIV1 in M.rosenbergii by activating the organism’s innate immune response and suppressing virus replication.This study provides a theoretical basis for utilizing thermal therapy in the management of viral infections in M.rosenbergii breeding programs,thereby facilitating the development of new strains resistant to DIV1.