Dovetree (Davidia involucrate), one of the first-class protective plants in China, is a relic species of paleotropical kingdom in Tertiary period, belonging toDavidiaceae with a single genus. In recent years, the incr...Dovetree (Davidia involucrate), one of the first-class protective plants in China, is a relic species of paleotropical kingdom in Tertiary period, belonging toDavidiaceae with a single genus. In recent years, the increase of regional developing projects has led to a sharp decrease of this ancient and rare plant in naturally distributed habitats and natural population. In this paper, the biological and ecological characteristics of Dovetree were described, and the geographically distributed condition of this species in different geologic periods as well as the major reasons for dynamic changes of its population were systematically analyzed in accordance with field researches and references. The investigations conducted in different years by setting up standard sample plots showed that the population number of Dovetree increased yearly in the areas without human interference but decreased yearly in the areas with human interference. With the increase of population density and human activities decreased as the logarithmic curve:Y=?100.7ln(x)+178.09, and it has turned from a dominant population into an endangered population. Based on the former and present studies on Dovetree, some protection strategies were put foreword for protecting this ancient and rare species. Keywords Davidia involucrate - Dovetree - Geographical Distribution - Biological Characteristics - Protection Policy CLC number S718.4 Document code A Article ID 1007-662X(2004)01-0039-06 Foundation Item: This research was coordinated by the National Natural Science Foundation of China (No. 39970591)Biography: Wu Gang (1965-), Male, Professor in Key Laboratory of Systems Ecology, Research Center for Eco Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.Responsible editor: Chai Ruihai展开更多
The identification and understanding of cryptic intraspecific evolutionary units(lineages) are crucial for planning effective conservation strategies aimed at preserving genetic diversity in endangered species.However...The identification and understanding of cryptic intraspecific evolutionary units(lineages) are crucial for planning effective conservation strategies aimed at preserving genetic diversity in endangered species.However, the factors driving the evolution and maintenance of these intraspecific lineages in most endangered species remain poorly understood. In this study, we conducted resequencing of 77 individuals from 22 natural populations of Davidia involucrata, a “living fossil” dove tree endemic to central and southwest China. Our analysis revealed the presence of three distinct local lineages within this endangered species, which emerged approximately 3.09 and 0.32 million years ago. These divergence events align well with the geographic and climatic oscillations that occurred across the distributional range.Additionally, we observed frequent hybridization events between the three lineages, resulting in the formation of hybrid populations in their adjacent as well as disjunct regions. These hybridizations likely arose from climate-driven population expansion and/or long-distance gene flow. Furthermore, we identified numerous environment-correlated gene variants across the total and many other genes that exhibited signals of positive evolution during the maintenance of two major local lineages. Our findings shed light on the highly dynamic evolution underlying the remarkably similar phenotype of this endangered species. Importantly, these results not only provide guidance for the development of conservation plans but also enhance our understanding of evolutionary past for this and other endangered species with similar histories.展开更多
Davidia involucrata is a rare endangered species belonging to the paleo-tropical flora of the Tertiary period, endemic to China, and has an important protection and ornamental value. However, the natural populations a...Davidia involucrata is a rare endangered species belonging to the paleo-tropical flora of the Tertiary period, endemic to China, and has an important protection and ornamental value. However, the natural populations and distribution areas of this ancient plant have been decreased sharply, because it suffers much disturbance from human activity in many regions. In this paper, data on Davidia involucrata forest in its most natural distribution areas were collected;the percentages of geographical areal types of seed-plant genera (Sensu Wu 1991) were summed;the community types were summarized using Braun-Blanquet methodology;and the geographical differentiation among communities was analyzed by detrended canonical correspondence analysis. The analysis of geographical types shows that temperate species are dominant and that tropical species also make up a high percentage. This indicates that Davidia involucrata forest is mainly temperate vegetation. In total, 16 communities were recognized by classification, and these communities were usually dominated by deciduous broad-leaved species, sometimes mixed with some evergreen species. The shrub layer of Davidia involucrata forest is generally occupied by bamboos. The geographical patterns among the communities are influenced mainly by temperature and moisture availability, and the forest is most likely to grow in warm, humid habitats.展开更多
In order to estimate the most effect stage and process on population growth and effective conserve the rare endangered plant Davidia involucrata, we analyzed the dynamics and the contributions of life-history componen...In order to estimate the most effect stage and process on population growth and effective conserve the rare endangered plant Davidia involucrata, we analyzed the dynamics and the contributions of life-history components on population dynamics based on Lefkovitch matrix model and sensitivity analysis. The life cycle of Davidia involucrata was divided into six stages (seed, seedling, juvenile, immature, early adult and late adult) based on the species characteristics and published literature data, the survival rates in each life-history stage were simulated using a static life table, and the fecundity of each stage was determined according to sample information. The results showed that the structure of the observed population was not ideal, and the numbers of seedlings and coppice shoots was similar. The population growth rate was influenced largely by individual growth process, and asexual reproduction made a larger contribution to population growth than sexual reproduction. However, sexual reproduction was more important than asexual reproduction, because most asexual reproducing individuals (the coppice shoots) were derived mainly from human destruction (e.g. felling trees). The most important stage was stage V (late adult), associated with seed production and germination. Therefore, conservation of Davidia involucrata populations should focus on stage V and sexual reproduction, in order to improve the seed production and germination rate, and to promote population stability and development.展开更多
[Objective]The aim was to isolate and identify an endophytic bacteria strain with antimicrobial activity from Davidia involucrate Brail.[Method]Endophytic strain with antibiosis ability was isolated from D.involucrate...[Objective]The aim was to isolate and identify an endophytic bacteria strain with antimicrobial activity from Davidia involucrate Brail.[Method]Endophytic strain with antibiosis ability was isolated from D.involucrate Brail by using cylinder-plate method.Then,it was identified through physiological and biochemical tests,16S rDNA homology analysis as well as some gene-specific sequence analysis.[Result]B221 stain had antimicrobial activity against a variety of rice plant pathogens,and it was identified as Bacillus subtilis.[Conclusion]This study enriches the research on endophyte within D.involucrate Brail,application of Bacillus bio-control,and therefore has laid a good foundation for the development of fungus used in biological control of crop pathogens.展开更多
A cold-induced plasma membrane protein which plays a protective role in avoiding freezing injury for some plants is one of hydrophobic proteins.A gene,named as DiRCI,was isolated from the cDNA library of Davidia invol...A cold-induced plasma membrane protein which plays a protective role in avoiding freezing injury for some plants is one of hydrophobic proteins.A gene,named as DiRCI,was isolated from the cDNA library of Davidia involucrata,containing an open reading frame of 174 bp flanked by a 92 bp 5′-untranslated sequence and a long 273 bp 3′-non-coding region.Alignment analysis indicated that the deduced amino acid sequence of 57 amino acids was high conserved with the MpRCI from Plantago asiatica,and the identity was 89.5%.Semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR)analysis showed that DiRCI wasn′t detected in various organs of D.involucrata above 25℃.When the plant grew under 8℃,the gene expressed in leaves,leafstalk and seeds,but not the roots.The expression level gradually increased after 4 h of the cold treatment and reached a maximum during 24-48 h,and then decreased.The results indicated the gene did have a relation with cold stress in plant and was predicted to be one DiRCI gene.The present data not only enrich gene resources of D.involucrata,but also laid a foundation for the research on molecular mechanism of plant acclimation to cold stress.展开更多
为探究珙桐(Davidia involucrata)幼苗生长所需氮(N)、磷(P)、钾(K)的最佳施肥量,该研究以1年生珙桐幼苗为对象,通过盆栽控制试验,以黄棕壤为基质,测定其基本理化指标,采用“3414”测土配方施肥法设置N、P、K三个因素,0、1、2、3四个水...为探究珙桐(Davidia involucrata)幼苗生长所需氮(N)、磷(P)、钾(K)的最佳施肥量,该研究以1年生珙桐幼苗为对象,通过盆栽控制试验,以黄棕壤为基质,测定其基本理化指标,采用“3414”测土配方施肥法设置N、P、K三个因素,0、1、2、3四个水平共14个处理(T1-T14),测定各处理生长条件下幼苗与叶、根生态化学计量特征指标,统计分析各指标的处理间差异及其相关关系。结果表明:(1)各处理珙桐幼苗叶和根的C含量差异较小;N、P含量表现出显著性差异(P<0.05),分别与N、P施肥水平呈正相关。(2)T2(N0P2K2)下叶和根的C∶N、N∶P失衡,限制了珙桐幼苗生长;各处理下叶和根的N∶P处于4.86至13.65之间,均低于14,受到N的限制。(3)比叶重、总生物量、株高增长量、地径增长量均表现为T11(N3P2K2)最低,分别比T1(N0P0K0)低6.78%、32.88%、18.91%、6.82%,T13(N1P2K1)促生长效果最显著,分别比T1高12.39%、82.37%、201.75%、120.45%。(4)相关分析显示,比叶重与根的N含量、叶和根的N∶P呈显著正相关(P<0.05),总生物量与根的C含量呈显著正相关(P<0.05),生长特征与化学计量特征之间存在一定关联性。(5)多重比较结果显示,N对各生长特征及根的C、N含量和叶的N、P含量有显著影响(P<0.05),P对比叶重、株高增长量和地径增长量,以及叶和根的P含量有显著影响(P<0.05),K对比叶重、株高增长量、地径增长量有显著影响(P<0.05)。综上认为,盆栽珙桐幼苗应注重N、P、K的均衡施用,避免因N施加过量(7.95 g·plant-1)而造成抑制作用,T13(2.65 g N、1.00 g P2O5、1.50 g K2O)是珙桐幼苗生长的最佳施肥方案。该研究构建的珙桐幼苗精准施肥模式,为珙桐种群扩繁及自然回归提供了核心技术支撑,助力濒危物种资源保育。展开更多
As climate change accelerates,plant species largely rely on genetic variation to adapt and survive when they fail to track their ecological niches through range shifts.Predicted genomic vulnerability is able to identi...As climate change accelerates,plant species largely rely on genetic variation to adapt and survive when they fail to track their ecological niches through range shifts.Predicted genomic vulnerability is able to identify populations lacking the necessary genetic variation for climate change adaptation.However,the role of introgression in genomic vulnerability remains poorly explored.Here,we used the dove tree(Davidia involucrata),a relict species native to southwestern China,to test whether introgression may reduce genomic vulnerability.By integrating population genomics and environmental data collected from 196 individuals of 18 populations,we identified 747 strictly climate-associated loci across the distribution range of D.involucrata,138 of which were recovered from the genetically admixed populations.We estimated the genomic vulnerability for three genetic lineages and two admixed groups using the gradient forest approach,and found that eastern populations are likely to be at higher risk.The eastern admixed populations exhibited a significant reduction,with introgression from the southern lineage.Cumulative importance analysis showed moderate importance for introgressive loci along environmental gradients.This indicates that the introduction of novel alleles through introgression provides only a partial and insufficient counterbalance to the maladaptation observed in D.involucrata under climate change.Our study highlights the role of intraspecific introgression in response to climate change and emphasizes the importance of genomic vulnerability studies in informing conservation practices for relict and endangered species.展开更多
基金The National Natural Science Foundation of China (No. 39970591)
摘要Dovetree (Davidia involucrate), one of the first-class protective plants in China, is a relic species of paleotropical kingdom in Tertiary period, belonging toDavidiaceae with a single genus. In recent years, the increase of regional developing projects has led to a sharp decrease of this ancient and rare plant in naturally distributed habitats and natural population. In this paper, the biological and ecological characteristics of Dovetree were described, and the geographically distributed condition of this species in different geologic periods as well as the major reasons for dynamic changes of its population were systematically analyzed in accordance with field researches and references. The investigations conducted in different years by setting up standard sample plots showed that the population number of Dovetree increased yearly in the areas without human interference but decreased yearly in the areas with human interference. With the increase of population density and human activities decreased as the logarithmic curve:Y=?100.7ln(x)+178.09, and it has turned from a dominant population into an endangered population. Based on the former and present studies on Dovetree, some protection strategies were put foreword for protecting this ancient and rare species. Keywords Davidia involucrate - Dovetree - Geographical Distribution - Biological Characteristics - Protection Policy CLC number S718.4 Document code A Article ID 1007-662X(2004)01-0039-06 Foundation Item: This research was coordinated by the National Natural Science Foundation of China (No. 39970591)Biography: Wu Gang (1965-), Male, Professor in Key Laboratory of Systems Ecology, Research Center for Eco Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.Responsible editor: Chai Ruihai
基金supported by the Second Tibetan Plateau Scientific Expedition and Research program (No. 2019QZKK0502)Strategic Priority Research Program of Chinese Academy of Sciences (No. XDB31010300)+1 种基金Fundamental Research Funds for the Central UniversitiesInternational Collaboration 111 Program (BP0719040)。
摘要The identification and understanding of cryptic intraspecific evolutionary units(lineages) are crucial for planning effective conservation strategies aimed at preserving genetic diversity in endangered species.However, the factors driving the evolution and maintenance of these intraspecific lineages in most endangered species remain poorly understood. In this study, we conducted resequencing of 77 individuals from 22 natural populations of Davidia involucrata, a “living fossil” dove tree endemic to central and southwest China. Our analysis revealed the presence of three distinct local lineages within this endangered species, which emerged approximately 3.09 and 0.32 million years ago. These divergence events align well with the geographic and climatic oscillations that occurred across the distributional range.Additionally, we observed frequent hybridization events between the three lineages, resulting in the formation of hybrid populations in their adjacent as well as disjunct regions. These hybridizations likely arose from climate-driven population expansion and/or long-distance gene flow. Furthermore, we identified numerous environment-correlated gene variants across the total and many other genes that exhibited signals of positive evolution during the maintenance of two major local lineages. Our findings shed light on the highly dynamic evolution underlying the remarkably similar phenotype of this endangered species. Importantly, these results not only provide guidance for the development of conservation plans but also enhance our understanding of evolutionary past for this and other endangered species with similar histories.
摘要Davidia involucrata is a rare endangered species belonging to the paleo-tropical flora of the Tertiary period, endemic to China, and has an important protection and ornamental value. However, the natural populations and distribution areas of this ancient plant have been decreased sharply, because it suffers much disturbance from human activity in many regions. In this paper, data on Davidia involucrata forest in its most natural distribution areas were collected;the percentages of geographical areal types of seed-plant genera (Sensu Wu 1991) were summed;the community types were summarized using Braun-Blanquet methodology;and the geographical differentiation among communities was analyzed by detrended canonical correspondence analysis. The analysis of geographical types shows that temperate species are dominant and that tropical species also make up a high percentage. This indicates that Davidia involucrata forest is mainly temperate vegetation. In total, 16 communities were recognized by classification, and these communities were usually dominated by deciduous broad-leaved species, sometimes mixed with some evergreen species. The shrub layer of Davidia involucrata forest is generally occupied by bamboos. The geographical patterns among the communities are influenced mainly by temperature and moisture availability, and the forest is most likely to grow in warm, humid habitats.
摘要In order to estimate the most effect stage and process on population growth and effective conserve the rare endangered plant Davidia involucrata, we analyzed the dynamics and the contributions of life-history components on population dynamics based on Lefkovitch matrix model and sensitivity analysis. The life cycle of Davidia involucrata was divided into six stages (seed, seedling, juvenile, immature, early adult and late adult) based on the species characteristics and published literature data, the survival rates in each life-history stage were simulated using a static life table, and the fecundity of each stage was determined according to sample information. The results showed that the structure of the observed population was not ideal, and the numbers of seedlings and coppice shoots was similar. The population growth rate was influenced largely by individual growth process, and asexual reproduction made a larger contribution to population growth than sexual reproduction. However, sexual reproduction was more important than asexual reproduction, because most asexual reproducing individuals (the coppice shoots) were derived mainly from human destruction (e.g. felling trees). The most important stage was stage V (late adult), associated with seed production and germination. Therefore, conservation of Davidia involucrata populations should focus on stage V and sexual reproduction, in order to improve the seed production and germination rate, and to promote population stability and development.
基金Supported by the National Natural Science Foundation of China(No.30960011)
摘要[Objective]The aim was to isolate and identify an endophytic bacteria strain with antimicrobial activity from Davidia involucrate Brail.[Method]Endophytic strain with antibiosis ability was isolated from D.involucrate Brail by using cylinder-plate method.Then,it was identified through physiological and biochemical tests,16S rDNA homology analysis as well as some gene-specific sequence analysis.[Result]B221 stain had antimicrobial activity against a variety of rice plant pathogens,and it was identified as Bacillus subtilis.[Conclusion]This study enriches the research on endophyte within D.involucrate Brail,application of Bacillus bio-control,and therefore has laid a good foundation for the development of fungus used in biological control of crop pathogens.
基金The Key Chinese National Natrual Science Foundation(30670209)a project from Depart ment of Educationin Sichuan Province(2003A175)a Program for Science and Technology Development in Mianyang City Sichuan Province(2005BN001-1)
摘要A cold-induced plasma membrane protein which plays a protective role in avoiding freezing injury for some plants is one of hydrophobic proteins.A gene,named as DiRCI,was isolated from the cDNA library of Davidia involucrata,containing an open reading frame of 174 bp flanked by a 92 bp 5′-untranslated sequence and a long 273 bp 3′-non-coding region.Alignment analysis indicated that the deduced amino acid sequence of 57 amino acids was high conserved with the MpRCI from Plantago asiatica,and the identity was 89.5%.Semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR)analysis showed that DiRCI wasn′t detected in various organs of D.involucrata above 25℃.When the plant grew under 8℃,the gene expressed in leaves,leafstalk and seeds,but not the roots.The expression level gradually increased after 4 h of the cold treatment and reached a maximum during 24-48 h,and then decreased.The results indicated the gene did have a relation with cold stress in plant and was predicted to be one DiRCI gene.The present data not only enrich gene resources of D.involucrata,but also laid a foundation for the research on molecular mechanism of plant acclimation to cold stress.
摘要为探究珙桐(Davidia involucrata)幼苗生长所需氮(N)、磷(P)、钾(K)的最佳施肥量,该研究以1年生珙桐幼苗为对象,通过盆栽控制试验,以黄棕壤为基质,测定其基本理化指标,采用“3414”测土配方施肥法设置N、P、K三个因素,0、1、2、3四个水平共14个处理(T1-T14),测定各处理生长条件下幼苗与叶、根生态化学计量特征指标,统计分析各指标的处理间差异及其相关关系。结果表明:(1)各处理珙桐幼苗叶和根的C含量差异较小;N、P含量表现出显著性差异(P<0.05),分别与N、P施肥水平呈正相关。(2)T2(N0P2K2)下叶和根的C∶N、N∶P失衡,限制了珙桐幼苗生长;各处理下叶和根的N∶P处于4.86至13.65之间,均低于14,受到N的限制。(3)比叶重、总生物量、株高增长量、地径增长量均表现为T11(N3P2K2)最低,分别比T1(N0P0K0)低6.78%、32.88%、18.91%、6.82%,T13(N1P2K1)促生长效果最显著,分别比T1高12.39%、82.37%、201.75%、120.45%。(4)相关分析显示,比叶重与根的N含量、叶和根的N∶P呈显著正相关(P<0.05),总生物量与根的C含量呈显著正相关(P<0.05),生长特征与化学计量特征之间存在一定关联性。(5)多重比较结果显示,N对各生长特征及根的C、N含量和叶的N、P含量有显著影响(P<0.05),P对比叶重、株高增长量和地径增长量,以及叶和根的P含量有显著影响(P<0.05),K对比叶重、株高增长量、地径增长量有显著影响(P<0.05)。综上认为,盆栽珙桐幼苗应注重N、P、K的均衡施用,避免因N施加过量(7.95 g·plant-1)而造成抑制作用,T13(2.65 g N、1.00 g P2O5、1.50 g K2O)是珙桐幼苗生长的最佳施肥方案。该研究构建的珙桐幼苗精准施肥模式,为珙桐种群扩繁及自然回归提供了核心技术支撑,助力濒危物种资源保育。
基金funded by the Key Technology Research and Development Program of Zhejiang Province(No.2023C03138)the National Natural Science Foundation of China(No.32100164)the National Key Research and Development Program of China(No.2017YFA0605104).
摘要As climate change accelerates,plant species largely rely on genetic variation to adapt and survive when they fail to track their ecological niches through range shifts.Predicted genomic vulnerability is able to identify populations lacking the necessary genetic variation for climate change adaptation.However,the role of introgression in genomic vulnerability remains poorly explored.Here,we used the dove tree(Davidia involucrata),a relict species native to southwestern China,to test whether introgression may reduce genomic vulnerability.By integrating population genomics and environmental data collected from 196 individuals of 18 populations,we identified 747 strictly climate-associated loci across the distribution range of D.involucrata,138 of which were recovered from the genetically admixed populations.We estimated the genomic vulnerability for three genetic lineages and two admixed groups using the gradient forest approach,and found that eastern populations are likely to be at higher risk.The eastern admixed populations exhibited a significant reduction,with introgression from the southern lineage.Cumulative importance analysis showed moderate importance for introgressive loci along environmental gradients.This indicates that the introduction of novel alleles through introgression provides only a partial and insufficient counterbalance to the maladaptation observed in D.involucrata under climate change.Our study highlights the role of intraspecific introgression in response to climate change and emphasizes the importance of genomic vulnerability studies in informing conservation practices for relict and endangered species.