To investigate the expression characteristics of sex related gene of FTZ-F 1 in the half-smooth tongue-sole (Cynoglossus semilaevis), the homologue FTZ-F1 (hsFTZ-F1) full-length cDNA was isolated from the testis b...To investigate the expression characteristics of sex related gene of FTZ-F 1 in the half-smooth tongue-sole (Cynoglossus semilaevis), the homologue FTZ-F1 (hsFTZ-F1) full-length cDNA was isolated from the testis by homologous cloning, and the cDNA included the open reading frame and a 66bp 5'-UTR, along with a 1619bp 3'-UTR, encoding a predicted 485 amino acid protein. Sequence, tissue distribution and phylogenic analyses of the FTZ-F1 showed that the hsFTZ-F1 belonged to SF-1/Ad4BP group. The hsFTZ-F1 transcripts were highly abundant in the gonads, kidneys, brain and head-kidneys, but weakly in other tissues. However, the expression level in the brain and head-kidney of female was highly abundant than in the male. The hsFTZ-F1 expression was highly abundant in the embryo than in the larvae, which suggested that the hsFTZ-F1 may be involved in the organogenesis in the tongue sole.展开更多
LATEST NEWS FROM THE WORLD OF BUSINESS CHINA New FTZ Established China’s commerce ministry and Inner Mongolia officials on 10 April laid out details for developing the newly established China(Inner Mongolia)Pilot Fre...LATEST NEWS FROM THE WORLD OF BUSINESS CHINA New FTZ Established China’s commerce ministry and Inner Mongolia officials on 10 April laid out details for developing the newly established China(Inner Mongolia)Pilot Free Trade Zone(FTZ),pledging efforts to turn it into a hub of opening up and a model of high-quality development.Yuan Xiaoming,assistant minister of commerce,said efforts will focus on empowering and enhancing capabilities,while exploring a new model for connecting domestic and international markets.展开更多
基金Supported by grants from State 863 High-Technology R&D Project of China(2006AA10A403)Shandong Genetic Improvement Key Project for Agricultural OrganismDoctor Initial Funding of Guangdong Ocean University(0712103)
摘要To investigate the expression characteristics of sex related gene of FTZ-F 1 in the half-smooth tongue-sole (Cynoglossus semilaevis), the homologue FTZ-F1 (hsFTZ-F1) full-length cDNA was isolated from the testis by homologous cloning, and the cDNA included the open reading frame and a 66bp 5'-UTR, along with a 1619bp 3'-UTR, encoding a predicted 485 amino acid protein. Sequence, tissue distribution and phylogenic analyses of the FTZ-F1 showed that the hsFTZ-F1 belonged to SF-1/Ad4BP group. The hsFTZ-F1 transcripts were highly abundant in the gonads, kidneys, brain and head-kidneys, but weakly in other tissues. However, the expression level in the brain and head-kidney of female was highly abundant than in the male. The hsFTZ-F1 expression was highly abundant in the embryo than in the larvae, which suggested that the hsFTZ-F1 may be involved in the organogenesis in the tongue sole.
摘要LATEST NEWS FROM THE WORLD OF BUSINESS CHINA New FTZ Established China’s commerce ministry and Inner Mongolia officials on 10 April laid out details for developing the newly established China(Inner Mongolia)Pilot Free Trade Zone(FTZ),pledging efforts to turn it into a hub of opening up and a model of high-quality development.Yuan Xiaoming,assistant minister of commerce,said efforts will focus on empowering and enhancing capabilities,while exploring a new model for connecting domestic and international markets.