期刊文献+
任意字段
题名或关键词
题名
关键词
摘要
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息
共找到4,350篇文章
< 1 2 … 218 >
每页显示 20 50 100
已选0条
引用分析
参考文献
引证文献
 统计分析
检索结果
已选文献
显示方式：
考虑冲击温升效应的Kong-Fang混凝土材料本构模型 认领 引用
1
作者 杨尚彬 方秦 孔祥振 《陆军工程大学学报》 2026年第1期37-46,共10页
为解决超高速动能武器打击下混凝土热-力耦合毁伤问题,弥补现有主流本构模型无法描述热软化效应的缺陷,开展了考虑冲击温升效应的混凝土动态本构模型研究。以Kong-Fang流体弹塑性损伤模型为基础,分析冲击载荷下混凝土温升机理,提出冲击... 为解决超高速动能武器打击下混凝土热-力耦合毁伤问题,弥补现有主流本构模型无法描述热软化效应的缺陷,开展了考虑冲击温升效应的混凝土动态本构模型研究。以Kong-Fang流体弹塑性损伤模型为基础,分析冲击载荷下混凝土温升机理,提出冲击波作用下温度累积计算方法,引入高温与高应变率耦合效应,构建高温高应变率耦合动态本构模型;通过LS-DYNA用户材料子程序接口建模,并结合长杆弹超高速侵彻混凝土靶体试验进行验证。结果表明,所提模型能准确表征冲击温升及其对材料性能的影响,速度超过3 km/s时,考虑温升的侵彻深度预测值较原始模型增幅显著,5 km/s时达10.8%,且能精准模拟靶体损伤模式,为混凝土防护结构抗超高速打击设计提供了数据支撑。 展开更多
关键词 超高速侵彻 动态本构模型 热-力耦合 冲击温升 Kong-Fang模型
暂未订购 下载PDF
The application of traditional Chinese medicine in preventing disease progression:Past,present and future of“Ji Bing Fang Bian”theory 认领 引用
2
作者 Yuan Long Cong-Mei Zhang +9 位作者 Hong-Yu Deng Zi-Ning Peng Fa-Li Zha Qian-Qian Wan Fei-Tian Min Ning Wang Zhong-Yu Peng Huan-Tian Cui Wei-Bo Wen Tao Chen 《Traditional Medicine Research》 2026年第12期67-86,共20页
In the early stages of various diseases—such as COVID-19,influenza,dengue fever,diabetes,pulmonary hypertension and inflammation-driven disorders—failure to intervene promptly can trigger uncontrolled inflammatory r... In the early stages of various diseases—such as COVID-19,influenza,dengue fever,diabetes,pulmonary hypertension and inflammation-driven disorders—failure to intervene promptly can trigger uncontrolled inflammatory responses and multi-system dysfunction,initiating complex pathological cascades that substantially increase morbidity,complication burden,and risk of adverse outcomes.The traditional Chinese medicine(TCM)principle of“preventing disease progression after onset”(Ji Bing Fang Bian)emphasizes early therapeutic intervention to halt disease exacerbation and prevent severe complications.This concept aligns closely with the modern medical strategy of early intervention.This review adopts the TCM theoretical framework of“Ji Bing Fang Bian”to systematically delineate the dynamic progression patterns and key pathophysiological mechanisms underlying the aforementioned conditions.Furthermore,it highlights the unique advantages and clinical value of TCM interventions,which leverage multi-component,multi-target,and multi-pathway synergistic actions to effectively interrupt disease progression.By elucidating the contemporary scientific underpinnings of the“Ji Bing Fang Bian”paradigm,this work aims to provide evidence-informed,and future prospective for the early prevention of these diseases using TCM. 展开更多
关键词 “Ji Bing Fang Bian”theory traditional Chinese medicine disease management basic study clinical application
暂未订购 下载PDF
Regulatory effects of Dangua Fang on the NAMPT/NAD+/SIRT1 axis and amino acid metabolism in type 2 diabetic rats 认领 引用
3
作者 Zhuang Han Zhi-Ta Wang +1 位作者 Liu-Qing Yang Xian-Pei Heng 《World Journal of Diabetes》 SCIE 2026年第7期227-242,共16页
BACKGROUND Type 2 diabetes mellitus(T2DM)is not only a disorder of glucose and lipid metabolism,but also closely associated with disturbances in amino acid metabolism.Dangua Fang(DGF)is a traditional Chinese medicine ... BACKGROUND Type 2 diabetes mellitus(T2DM)is not only a disorder of glucose and lipid metabolism,but also closely associated with disturbances in amino acid metabolism.Dangua Fang(DGF)is a traditional Chinese medicine formula used for the treatment of diabetes and has been shown to regulate amino acid metabolism.The nicotinamide phosphoribosyltransferase(NAMPT)icotinamide adenine dinucleotide(NAD+)/sirtuin 1(SIRT1)axis is closely linked to amino acid metabolism and plays an important role in maintaining metabolic homeostasis.AIM To investigate the regulatory effects of DGF on the hepatic NAMPT/NAD+/SIRT1 axis and amino acid metabolism in T2DM rats.METHODS A T2DM rat model was established by a high-glucose and high-fat diet combined with streptozotocin injection.Rats were randomly assigned into four groups,namely the control group,model group,DGF group(DFG,20 g/kg),and metformin group(0.11 g/kg),with eight rats in each group.After 14 weeks of intervention,glucolipid metabolism-related indicators,expression of the NAMPT/NAD+/SIRT1 axis,and hepatic amino acid metabolism were evaluated.RESULTS Compared with the model group,the DFG and metformin groups exhibited significantly increased body weight and high-density lipoprotein cholesterol levels(P<0.05 and P<0.01),accompanied by reductions in fasting and postprandial blood glucose,liver weight,liver index,haemoglobin A1c,total cholesterol,triglycerides,low-density lipoprotein cholesterol,and free fatty acid levels(P<0.05 and P<0.01),along with alleviated hepatic histopathological damage and significantly upregulated NAMPT and SIRT1 mRNA and protein expression(P<0.05 and P<0.01),as well as elevated NAD+content(P<0.01).In the DFG group,several key metabolic pathways were significantly modulated,including riboflavin metabolism,pentose and glucuronate interconversions,purine metabolism,glycolysis/gluconeogenesis,and vitamin B6 metabolism,indicating significant modulation of amino acid metabolism.CONCLUSION DGF significantly ameliorates glucolipid metabolism in T2DM rats,an effect that may be mediated by regulation of the hepatic NAMPT/NAD+/SIRT1 axis and amino acid metabolism disorders. 展开更多
关键词 Traditional Chinese medicine Dangua Fang Type 2 diabetes mellitus Nicotinamide phosphoribosyltransferaseicotinamide adenine dinucleotide/sirtuin 1 axis Amino acid metabolism Targeted metabolomics
暂未订购 下载PDF
Medical and Humanistic Traits of Folk Proven Formulas:A Case of Shen Cha Zheng Ji Yan Fang 认领 引用
4
作者 LIU Yang WANG Lei YU Qi 《Chinese Medicine and Culture》 CAS 2026年第2期189-201,共13页
During the Republic of China,the Shanxi government invested substantial human and material resources to compile and publish Shen Cha Zheng Ji Yan Fang(《审查征集验方》Inspection of the Collection of TCM Proven Formula... During the Republic of China,the Shanxi government invested substantial human and material resources to compile and publish Shen Cha Zheng Ji Yan Fang(《审查征集验方》Inspection of the Collection of TCM Proven Formulas),the first official compilation of traditional Chinese medicine(TCM)folk proven formulas in modern China.Taking this book as the research object,this study explored its inherent medical and humanistic traits,and found that most folk proven formulas featured concise formulations,which were significantly different from classical TCM formulas.The average dosage of some medicinal materials exceeded the upper limit stipulated in the current pharmacopoeia of the People’s Republic of China.For the sake of easy use,folk proven formulas mostly adopted preparation methods such as pulverizing into powder and making into pills.In the context of folk medicinal material shortage or emergency,many medicine-food homologous items,condiments and daily necessities were also incorporated into the formulas.The study revealed that though the book involved a wide variety of medicinal materials,the high-frequency used ones were still consistent with those of TCM.Cluster analysis of different departments based on shared medicinal materials also indicated that folk proven formulas followed the traditional theories and treatment principles of TCM and were not divorced from the mainstream TCM system.The scope of treatments covered in the book indirectly reflected the social health characteristics of modern China,such as the frequent occurrence of infectious diseases and the widespread malnutrition of the people.Folk proven formulas verified to be effective through long-term and extensive practice also provided targeted references for the research and development of modern medicines. 展开更多
关键词 Folk proven formulas Shen Cha Zheng Ji Yan Fang(《(审查征集验方》Inspection of the Collection of TCM Proven Formulas) Humanistic traits Traditional Chinese medicine Big data
暂未订购 下载PDF
Jiedu Fang inhibits hypoxia-induced angiogenesis in hepatocellular carcinoma by targeting Aurora A/STAT3/IL-8 signaling pathway 认领 引用
5
作者 Mao-feng Zhong Yu-jun Luo +2 位作者 Yu-yu Guo Shuang Xiang Wan-fu Lin 《Journal of Integrative Medicine》 SCIE CAS CSCD 2025年第6期683-693,共11页
Objective:Angiogenesis is a critical target for hepatocellular carcinoma(HCC)treatment The previous studies indicated that Jiedu Fang(JDF)could inhibit hypoxia-induced angiogenesis through interleukin-8(IL-8).Therefor... Objective:Angiogenesis is a critical target for hepatocellular carcinoma(HCC)treatment The previous studies indicated that Jiedu Fang(JDF)could inhibit hypoxia-induced angiogenesis through interleukin-8(IL-8).Therefore,the present study further explores the mechanisms behind JDF's inhibition of HCC angiogenesis.Methods:Angiogenesis was assessed with the capillary-like tube formation assay in vitro and the matrigel plug angiogenesis assay in vivo.A liver cancer-related gene set and genes associated with angiogenesis and the hypoxic microenvironment were analyzed using a bioinformatics platform.Real-time reverse transcription-polymerase chain reaction and Western blotting assays were used to assess the targeted mRNA and protein levels,respectively.The Transwell assay was used to assess the migration and invasion potential of EA.hy 926 cells.The orthotopic tumor xenograft model was established,and immunohistochemistry and immunofluorescence assays were used to detect cluster of differentiation 31 and angiopoietin 2 expression,while an enzyme-linked immunosorbent assay was used to detect vascular endothelial growth factor and IL-8 protein levels.Results:In vitro and in vivo assays showed that IL-8 promoted angiogenesis,and JDF could antagonize this effect.Bioinformatics analysis indicated that aurora kinase A(Aurora A)was an important candidate,which can promote IL-8 expression through activation of signal transducer and activator of transcription3(STAT3).The overexpression of Aurora A increased IL-8 secretion and promoted HCC migration,invasion,and angiogenesis,which was partly inhibited by JDF.Such effects were validated by in vivo assays.Further validation using the STAT3 inhibitor S3I-201 demonstrated that STAT3 was regulated by Aurora A.Conclusion:JDF exhibits efficacy in reducing hypoxia-induced angiogenesis in HCC through a mechanism involving the Aurora A/STAT3/IL-8 signaling pathway.Therefore,JDF holds promise as a potential therapeutic approach for targeting HCC angiogenesis. 展开更多
关键词 Hepatocellular carcinoma Jiedu Fang Angiogenesis Aurora A Hypoxic microenvironment
暂未订购 下载PDF
Network pharmacology combined with ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry method to explore the mechanism of Shizhi Fang(矢志方)in treating uric acid nephropathy mice 认领 引用
6
作者 YANG Feng ZHANG Xuming +1 位作者 WU Zhiyuan GAO Jiandong 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2025年第6期1342-1352,共11页
OBJECTIVE:To elucidate the potential mechanisms of Shizhi Fang(SZF,矢志方)in the treatment of uric acid nephropathy(UAN).METHODS:SZF-containing serum was prepared from six male rats and analyzed using ultra-high-perfo... OBJECTIVE:To elucidate the potential mechanisms of Shizhi Fang(SZF,矢志方)in the treatment of uric acid nephropathy(UAN).METHODS:SZF-containing serum was prepared from six male rats and analyzed using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS).Network pharmacology was employed was integrated with UPLC-Q-TOF-MS to predict SZF targets for the treatment of UAN,which were subsequently validated through in vivo experiments.Sixty male Bagg Albino Laboratory-Bred Mouse,substrain c mice were randomly allocated into six groups:Normal,Model,Febuxostat,and three SZF dosage groups.Except for the Normal group,all mice were administered potassium oxonate(250 mg/kg)and adenine(50 mg/kg)via gavage to induce UAN.Four hours postadministration,the Febuxostat group received Febuxostat(6 mg/kg),while the SZF groups received low(0.234 g/kg),medium(0.468 g/kg),or high(0.936 g/kg)doses of SZF.The Normal and Model groups were given an equivalent volume of saline.All treatments were conducted over a period of four weeks.Urine and blood samples were collected for biochemical analysis,and kidney tissues were subjected to histopathological examination and Western blot analysis.RESULTS:Nine prototype compounds and 30 metabolites were identified in SZF serum.Network pharmacology analysis revealed 195 drug targets and 1608 disease targets,with 76 common drug-disease targets,including signal transducer and activator of transcription 3(STAT3),proto-oncogene tyrosine-protein kinase Src(SRC),matrix metalloproteinase-9(MMP9),Caspase 3,and toll-like receptor 4(TLR4)as key targets.Gene Ontology analysis identified 325 biological processes,48 cellular components,and 72 molecular functions,while Kyoto Encyclopedia of Genes and Genomes analysis identified 113 pathways.Molecular docking demonstrated strong binding affinities between active compounds and their targets.In the animal study,SZF treatment alleviated pathological damage and improved serum and urine biochemical markers compared to the Model group(P<0.05,P<0.01,P<0.001).Western blot analysis showed a significant reduction in phosphorylated-STAT3,phosphorylatedSRC,MMP9,TLR4,and Caspase3 expression in renal tissues of SZF-treated mice(P<0.001).CONCLUSION:SZF may exert therapeutic effects on UAN through multiple targets and pathways. 展开更多
关键词 uric acid nephropathy liquid chromatography-mass spectrometry mechanics network pharmacology Shizhi Fang
暂未订购 下载PDF
Yan Yonghe and his Formulas for Succouring the Sick(Ji Sheng Fang) 认领 引用
7
作者 Yongxuan Liang Yinghua Huang 《Journal of Traditional Chinese Medical Sciences》 CAS CSCD 2025年第3期317-318,共2页
Yan Yonghe,also known as Yan Zili(1199-1267),was a famous physician of the South Song Dynasty.He lived in Nankang(present Jiujiang,Jiangxi province),and called himself“a Native of Lushan Mountain”because he lived th... Yan Yonghe,also known as Yan Zili(1199-1267),was a famous physician of the South Song Dynasty.He lived in Nankang(present Jiujiang,Jiangxi province),and called himself“a Native of Lushan Mountain”because he lived there for a long time.In the preface of the Formulas for Succouring the Sick(Ji Sheng Fang),he said he loved study since childhood,and when he was 12 years old he started to learn medicine. 展开更多
关键词 Lushan Mountain South Song Dynasty Yan Yonghe Ji Sheng Fang Physician
暂未订购 下载PDF
Review on the research progress into the traditional Chinese medicinal Jing Fang granules:chemical composition,pharmacological effects and clinical applications 认领 引用
8
作者 Rui Zhao Tai-Li Zhao +6 位作者 Hong-Hua Li Pei-Pei Zhao Meng-Meng Wang Yi-Wei Meng Jing-Li Ren Xin Yin Jing-Chun Yao 《Integrative Medicine Discovery》 2025年第6期1-13,共13页
Jing Fang granules(JFG)are a modern Chinese medicinal formulation derived from Jing Fang Defeating Toxin San(JFDS).JFG primarily contains bioactive compounds such as flavonoids/flavonoid glycosides,coumarins and other... Jing Fang granules(JFG)are a modern Chinese medicinal formulation derived from Jing Fang Defeating Toxin San(JFDS).JFG primarily contains bioactive compounds such as flavonoids/flavonoid glycosides,coumarins and other components,which have various activities:anti-inflammatory,immune system regulation,antiviral,anti-aging,detoxification,and protection of the liver,lungs,and kidneys.JFG has broad potential therapeutic applications.The mechanisms of JFG’s actions are primarily associated with signaling pathways such as TLR4/NF-κB,PI3K/AKT,and MAPK.This article reviews the research progress on JFG,including chemical composition,pharmacologic effects,mechanism of action,safety,clinical application,etc.,serving as a foundation for future research and clinical applications. 展开更多
关键词 chemical composition clinical application Jing Fang granules pharmacological effect traditional Chinese herbal formula
暂未订购 下载PDF
Efficacy and Safety of Miao Medicine Jinlong Zhi Xie Fang Enema in the Treatment of Ulcerative Colitis (Large Intestine Damp-Heat Syndrome): A Randomized Controlled Trial 认领 引用
9
作者 Chongwu Che Yingxiang Yang +3 位作者 Liuying Cao Qin Yang Linmin Teng Wang Long 《Journal of Clinical and Nursing Research》 2025年第5期19-27,共9页
Ulcerative colitis(UC)is a chronic inflammatory bowel diseasethat significantly affects the quality of life of patients.Traditional treatments often have limitations,and alternative therapies are being explored.Miao M... Ulcerative colitis(UC)is a chronic inflammatory bowel diseasethat significantly affects the quality of life of patients.Traditional treatments often have limitations,and alternative therapies are being explored.Miao Medicine,particularly Jinlong Zhi Xie Fang Enema,is a traditional herbal remedy used to treat UC symptoms,especially in patients with Large Intestine Damp-Heat Syndrome.However,clinical evidence supporting its efficacy is limited. 展开更多
关键词 Ulcerative colitis Jinlong Zhi Xie Fang Miao Medicine Intestinal mucosal barrier Randomized controlled trial Herbal medicine
暂未订购 下载PDF
Thoughts on Coronavirus Disease 2019 Based on JingFang Medicine (Classical Chinese Formula) Solutions for COVID-19 认领 引用
10
作者 Huang Huang 《Chinese Medicine and Culture》 2019年第1期115-120,共6页
This article aims to provide some thoughts on the prevention and treatment of Coronaviras Disease 2019(COVTD-19)from the perspective of JingFang Medicine(Classical Chinese Formula).It is believed that the vague theore... This article aims to provide some thoughts on the prevention and treatment of Coronaviras Disease 2019(COVTD-19)from the perspective of JingFang Medicine(Classical Chinese Formula).It is believed that the vague theoretical understanding of COVTD-19 in Traditional Chinese Medicine does not hinder the precise treatment of the disease by following the rale of“With this Zheng,prescribe this Fang.”According to the principle of“Fang-Zheng Correlation”and the knowledge gained from the thousands of years of experience in treating febrile diseases,Xiao Chai Hu Decoction(小柴胡汤)and its modifications are recommended with the emphasis on individualized treatment.As another form of practicing“Fang-Zheng Correlation,”generalized group treatment should also be paid attention to.Giving considerations to(ire historical medical data,Jing Fang Bai Du Powder(荆防败毒散)and Shi Shen Decoction(十神汤)are reconunended for group prevention treatment.Assisting the Zheng(Upright)Qi and using tonic formulas are two entirely different concepts.According to the principle of“Fang-Zheng Correlation,”tonics abuse should be avoided in the prevention of COVID-19,and the using of Huang Qi(黄芪Radix Astragali seu Hedysari)should also be very carefully done. 展开更多
关键词 Classical formula coronavirus disease 2019(COVID-19) Fang-Zheng correlation Huang Qi Jing Fang Bai Du Powder Radix Astragali seu Hedysari Xiao Chai Hu Decoction
暂未订购 下载PDF
Tong xie yao fang relieves irritable bowel syndrome in rats via mechanisms involving regulation of 5-hydroxytryptamine and substance P 认领 引用 被引量：36
11
作者 Yue Yin Lei Zhong +3 位作者 Jian-Wei Wang Xue-Ying Zhao Wen-Jing Zhao Hai-Xue Kuang 《World Journal of Gastroenterology》 SCIE CAS 2015年第15期4536-4546,共11页
AIM: To investigate whether the Chinese medicine Tong Xie Yao Fang(TXYF) improves dysfunction in an irritable bowel syndrome(IBS) rat model. METHODS: Thirty baby rats for IBS modeling were separated from mother rats(1... AIM: To investigate whether the Chinese medicine Tong Xie Yao Fang(TXYF) improves dysfunction in an irritable bowel syndrome(IBS) rat model. METHODS: Thirty baby rats for IBS modeling were separated from mother rats(1 h per day) from days 8 to 21, and the rectum was expanded by angioplasty from days 8 to 12. Ten normal rats were used as normal controls. We examined the effects of TXYF on defection frequency, colonic transit function and smooth muscle contraction, and the expression of 5-hydroxytryptamine(5-HT) and substance P(SP) in colonic and hypothalamus tissues by Western blot and RT-PCT techniques in both normal rats and IBS model rats with characterized visceral hypersensitivity.in normal rats and 4.5 ± 1.58 in IBS model rats(P < 0.001). However, the defecation frequency was significantly decreased(3.0 ± 1.25 vs 4.5 ± 1.58, P < 0.05), while the time(in seconds) of colon transit function was significantly increased(256.88 ± 20.32 vs 93.36 ± 17.28, P < 0.001) in IBS + TXYF group rats than in IBS group rats. Increased colonic smooth muscle tension and contract frequency in IBS model rats were significantly decreased by administration of TXYF. Exogenous agonist stimulants increased spontaneous activity and elicited contractions of colon smooth muscle in IBS model rats, and all of these actions were significantly reduced by TXYF involving 5-HT and SP down-regulation. CONCLUSION: TXYF can modulate the activity of the enteric nervous system and alter 5-HT and SP activities, which may contribute to the symptoms of IBS. 展开更多
关键词 Tong Xie Yao Fang Irritable bowel syndrome 5-hydroxytryptamine Substance P Traditional Chinesemedicine
暂未订购 下载PDF
Effect of Tong Xie Yao Fang on endogenous metabolites in urine of irritable bowel syndrome model rats 认领 引用 被引量：19
12
作者 Xue-Ying Zhao Jian-Wei Wang +3 位作者 Yue Yin Kai Li Miao Zhang Fu-Ping Yan 《World Journal of Gastroenterology》 SCIE CAS 2019年第34期5134-5151,共18页
BACKGROUND Tong Xie Yao Fang is a representative traditional Chinese prescription for the treatment of liver and spleen deficiency,abdominal pain and diarrhea.It has a unique function in the treatment of gastrointesti... BACKGROUND Tong Xie Yao Fang is a representative traditional Chinese prescription for the treatment of liver and spleen deficiency,abdominal pain and diarrhea.It has a unique function in the treatment of gastrointestinal dysfunction including irritable bowel syndrome(IBS),is a common functional bowel disease.Its main symptoms are recurrent abdominal pain,diarrhea,constipation or alternations between diarrhea and constipation.There are obvious differences in metabolites between TCM syndromes.By comparing the body fluid metabolism maps of model animals,metabolomics can discover disease biomarkers,analyze the differences in metabolic pathways and understand the pathological process and the metabolic pathways of substances in the body.Thus,the evaluation of animal models tends to be comprehensive and objective.This may provide further understanding between the interaction between Tong Xie Yao Fang and the IBS model.AIM To evaluate the effect of Tong Xie Yao Fang on IBS rats by using metabolomics method.METHODS Wistar rats were used to establish IBS models,and then randomly divided into four groups:A model control group and three Tong Xie Yao Fang treatment groups(high,medium and low doses).A normal,non-IBS group was established.The rats were treated for 2 wk.On days 0 and 14 of the experimental model,urine was collected for 12 h and was analyzed by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry.Nine potential biomarkers were identified,and six major metabolic pathways were found to be related to IBS.RESULTS In the study of metabonomics,nine potential biomarkers including L-serine,4-methylgallic acid,L-threonine,succinylacetone,prolyl-hydroxyproline,valylserine,acetyl citrate,marmesin rutinoside and 5-hydroxy-L-tryptophan were identified in urine,which were assigned to amino acids,organic acids,succinyl and glycosides.Furthermore,the metabolic pathway of L-serine,L-threonine and 5-hydroxy-L-tryptophan was found in the Kyoto Encyclopedia of Genes and Genomes,which mainly involved the metabolism of cysteine and methionine,vitamin B6 metabolism,serotonin synapse,tryptophan metabolism,sphingolipid metabolism,digestion,absorption of protein and amino acid metabolism.These pathways are related to intestinal dysfunction,inflammatory syndrome,nervous system dysfunction and other diseases.CONCLUSION Tong Xie Yao Fang has pharmacological effects on IBS,and its mechanism may be related to the metabolism of the nine potential biomarkers identified above in urine. 展开更多
关键词 Tong Xie Yao Fang Irritable bowel syndrome Liver-spleen disharmony Endogenous metabolites Metabolomics
暂未订购 下载PDF
Mechanisms of Dangua Fang(丹瓜方)in multi-target and multi-method regulation of glycolipid metabolism based on phosphoproteomics 认领 引用 被引量：7
13
作者 HENG Xianpei WANG Zhita +4 位作者 LI Liang YANG Liuqing HUANG Suping JIN Lang HE Weidong 《Journal of Traditional Chinese Medicine》 SCIE CSCD 2024年第2期334-344,共11页
OBJECTIVE:To explore the mechanism of Dangua Fang(丹瓜方,DGR)in multi-target and multi-method regulation of glycolipid metabolism based on phosphoproteomics.METHODS:Sprague-Dawley rats with normal glucose levels were ... OBJECTIVE:To explore the mechanism of Dangua Fang(丹瓜方,DGR)in multi-target and multi-method regulation of glycolipid metabolism based on phosphoproteomics.METHODS:Sprague-Dawley rats with normal glucose levels were randomly divided into three groups,including a conventional diet control group(Group A),high-fat-highsugar diet model group(Group B),and DGR group(Group C,high-fat-high-sugar diet containing 20.5 g DGR).After 10 weeks of intervention,the fasting blood glucose(FBG),2 h blood glucose[PBG;using the oral glucose tolerance test(OGTT)],hemoglobin A1c(HbA1c),plasma total cholesterol(TC),and triglycerides(TG)were tested,and the livers of rats were removed to calculate the liver index.Then,hepatic portal TG were tested using the Gross permanent optimization-participatiory action planning enzymatic method and phosphoproteomics was performed using liquid chromatography with tandem mass spectrometry(LC-MS/MS)analysis followed by database search and bioinformatics analysis.Finally,cell experiments were used to verify the results of phosphoproteomics.Phosphorylated mitogen-activated protein kinase kinase kinase kinase 4(MAP4k4)and phosphorylated adducin 1(ADD1)were detected using western blotting.RESULTS:DGR effectively reduced PBG,TG,and the liver index(P1.2),DGR upregulated the modification of 228 phosphorylation sites involving 204 corresponding function proteins,and downregulated the modification of 358 phosphorylation sites involving 358 corresponding function proteins,which included correcting 75 phosphorylation sites involving 64 corresponding function proteins relating to glycolipid metabolism.Therefore,DGR improved biological tissue processes,including information storage and processing,cellular processes and signaling,and metabolism.The metabolic functions regulated by DGR mainly include energy production and conversion,carbohydrate transport and metabolism,lipid transport and metabolism,inorganic ion transport and metabolism,secondary metabolite biosynthesis,transport,and catabolism.In vitro phosphorylation validation based on cell experiments showed that the change trends in the phosphorylation level of MAP4k4 and ADD1 were consistent with that of previous phosphoproteomics studies.CONCLUSION:DGR extensively corrects the modification of phosphorylation sites to improve corresponding glycolipid metabolism-related protein expression in rats with glycolipid metabolism disorders,thereby regulating glycolipid metabolism through a multi-target and multi-method process. 展开更多
关键词 phosphoproteomics glycolipid metabolism therapeutic mechanism compound formula Dangua Fang
暂未订购 下载PDF
Dangua Fang(丹瓜方)regulating tricarboxylic acid cycle and respiratory chain and its mechanism in diabetic rats 认领 引用 被引量：7
14
作者 HENG Xianpei WANG Zhita +2 位作者 YANG Liuqing LI Liang HUANG Suping 《Journal of Traditional Chinese Medicine》 SCIE CSCD 2023年第6期1150-1159,共10页
OBJECTIVE:To investigate the influence and possible targets of Dangua Fang(丹瓜方)on tricarboxylic acid(TCA)cycle and respiratory chain to enrich the prescription’s mechanism of effective intervention on glycolipid m... OBJECTIVE:To investigate the influence and possible targets of Dangua Fang(丹瓜方)on tricarboxylic acid(TCA)cycle and respiratory chain to enrich the prescription’s mechanism of effective intervention on glycolipid metabolic diseases such as type 2 diabetes.METHODS:After interventional rats were fed with high glucose and high fat diet ad libitum for 4 weeks,intraperitoneally injected streptozotocin to induce diabetic model.According to blood glucose level,28 diabetic rats were selected and continued to be fed with high glucose and high fat diet,were stratified by body weight,and divided randomly by blood glucose into Model group(was given sterile water by gastric perfusion and injected aquae pro injection intraperitoneally),Dangua group[Dangua liquor(丹瓜方液)20.5 g·kg-1·d-1 by perfusion and aquae pro injection intraperitoneally],Inhibitor group[sterile water by perfusion and nicotinamide phosphoribosyl transferase(Nampt)specific blocker GEN-6171.25 mg/kg intraperitoneally],DanInhit group(Dangua liquor and GEN-617 synchronously).Control group were continuously fed with ordinary diet.The intervention was last for 10 weeks.Body weight(BW),liver index(LI),glycosylated hemoglobin(HbA1c),TC,TG,free fatty acids(FFA),creatinine(Cr),and A-ketoglutarate(α-KG),Iso-citric acid(ICA),oxaloacetic acid(OAA)were tested.The cytochrome C oxidase(COX)and Succinate dehydrogenase(SDH)were evaluated by Colorimetry;Nampt protein,Adenosine triphosphate(ATP)synthase(ATPs),Nicotinamide adenine dinucleotide(NAD+)and its reduced(NADH)in liver were measured by enzyme linked immunosorbent assay.The expressions of Nampt and mitochondrialnadhdehydrogenase-1(mt-ND1)gene in liver was assessed by real-time polymerase chain reaction.Hepatic tissue staining was also completed.RESULTS:The levels of BW,ICA,α-KG and Nampt-mRNA in the Model group are lower than that in the Normal group(P<0.05),conversely,liver weight,LI,TC,HbA1c,SDH and ATPs,mt-ND1-mRNA,and Nampt protein in the Model group are higher(P<0.01,P<0.05).Compared with Model group,the levels of ICA,Nampt-mRNA and Nampt in Dangua group are significantly increased,and FFA obviously raised(P<0.01 and P<0.05);liver weight,BW,SDH are obviously lower,and HbA1c decreased significantly(P<0.01,P<0.05).TG,FFA and Nampt protein increased in the DanInhit group,TC,TG,BW obviously increased in the Inhibitor group,but SDH is decreased in both the two groups(P<0.05,P<0.01).Compared with Dangua group,DanInhib group has the lower levels of ICA,mt-ND1-mRNA,Nampt-mRNA,and the higher level of BW,LI and HbA1c.In the Inhibitor group,ICA and Nampt protein decreased,BW and LI,HbA1c and TG increased(P<0.01 or P<0.05).Tissue staining display that,in the model group there is obvious pathologic changes ie:fibrosis,steatosis and inflammatory cell infiltration.Lesions in the Dangua group are mild,and those of Inhibitor group are more obvious than the Model group,and DanInhit group is intermediately affected compared to Dangua group and Inhibitor group.CONCLUSION:Dangua Fang increases the metabolic flux of TCA cycle and optimizes respiratory chain function by up-regulating Nampt expression. 展开更多
关键词 Dangua Fang diabetes mellitus citric acid cycle electron transport nicotinamide phosphoribosyltransferase
暂未订购 下载PDF
Wenyang Huazhuo Fang exerts transient receptor potential cation channel subfamily C member-dependent nephroprotection in a rat model of doxorubicin-induced nephropathy 认领 引用 被引量：7
15
作者 An Peng Dong Sheng +5 位作者 Li Xingyao Cai Zimo Ye Bingyu Zhang Aijun Shi Xingmin Wu Xili 《Journal of Traditional Chinese Medicine》 SCIE CSCD 2020年第4期613-620,共8页
OBJECTIVE:To determine the effect of Wenyang Huazhuo Fang(WHF),a Traditional Chinese Medicine decoction,on renal function in a rat model of doxorubicin-induced nephropathy,and to elucidate the underlying mechanism.MET... OBJECTIVE:To determine the effect of Wenyang Huazhuo Fang(WHF),a Traditional Chinese Medicine decoction,on renal function in a rat model of doxorubicin-induced nephropathy,and to elucidate the underlying mechanism.METHODS:Sprague-Dawley rats were randomly divided into six groups:control,doxorubicin-nephropathy,and prednisone-treated(6.45 mg·kg^-1·d^-1)doxorubicin nephropathy groups,as well as high-(7.26 g·kg^-1·d^-1),medium-(2.42 g·kg-1·d-),and low-dose(0.81 g·kg^-1·d^-1)WHF-treated doxorubicin-nephropathy groups.The nephropathy rat model was established by two tail vein injections of doxorubicin,followed by prednisone or WHF treatment for 8 weeks.Body weights were monitored and urinary protein was measured every 2 weeks.After the end of the treatment period,the rats were euthanized.Serum biochemical indicators were determined and renal morphological alterations were assessed using histological staining.The expression of transient receptor potential cation channel subfamily C member 6(TRPC6),stromal interaction molecule 1(STIM1),and calcium release-activated calcium channel protein 1(Orai1)was detected using western blotting,and their mRNA levels were examined using quantitative real-time reverse transcription-polymerase chain reaction.RESULTS:WHF treatment was found to significantly ameliorate weight loss,proteinuria,hypoalbuminemia,and dyslipidemia in doxorubicin-nephropathy rats.The protein and mRNA levels of TRPC6,STIM1,and Orai1 were partially,but significantly suppressed by prednisone or WHF treatment.CONCLUSION:Treatment with WHF significantly ameliorates renal injury in a rat model of doxorubicin-induced nephropathy,which could be at least partially related to repression of the TRPC6 pathway. 展开更多
关键词 Nephropathy Doxorubicin TRPC6 cation channel Stim1 protein,rat Orai1 protein,rat Wenyang Huazhuo Fang
暂未订购 下载PDF
Effect of Zibu Shenjing Fang(滋补肾精方) on Growth and Development of the Mouse with Kidney-essence Insufficiency and Study on the Mechanism 认领 引用 被引量：5
16
作者 沈世林 苏小军 +4 位作者 王玉萍 王文辉 时吉萍 姚军孝 强志鹏 《Journal of Traditional Chinese Medicine》 SCIE CSCD 2011年第3期232-234,共3页
Objective:To investigate effects of Zibu Shenjing Fang(滋补肾精方) on growth and development of the mouse with insufficiency of kidney-essence and the mechanism.Methods:Total 50 mice were randomly divided into a norma... Objective:To investigate effects of Zibu Shenjing Fang(滋补肾精方) on growth and development of the mouse with insufficiency of kidney-essence and the mechanism.Methods:Total 50 mice were randomly divided into a normal group,a model group,a Jingui Shenqi Wan(金匮肾气丸) group,a Zibu Shenjing Fang high dose group and a Zibu Shenjing Fang low dose group,10 mice in each group.The kidney-essence insufficiency mouse model was established by use of threat-injuring the kidney combined with over-fatigue.At the same time of modeling,the mice in the model group were intragastrically administrated with saline 20 mL·kg-1·d-1,in the Jingui Shenqi Wan group with suspension of the Jingui Shenqi Wan 2.7 g·kg-1·d-1,in the Zibu Shenjing Fang high dose group with Zibu Shenjing Fang 20 g·kg-1·d-1 and in the Zibu Shenjing Fang low dose group with Zibu Shenjing Fang 10 g·kg-1·d-1,for 21 consecutive days.The general state was observed,the body weight was weighted,and serum growth hormone(GH) and insulin-like growth factor-1(IGF-1) contents were detected.Results:Compared with model group,Zibu Shenjing Fang groups and Jingui Shenqi Wan group could improve manifestation of the mouse with kidney-essence insufficiency,increase body weight of the mouse and serum GH and IGF-1 contents,especially in the high dose group.Conclusion:Zibu Shenjing Fang gives play to the function of tonifying the kidney and replenishing essence through regulating GH and IGF-1 levels,so as to influence growth and development of the mouse. 展开更多
关键词 Zibu Shenjing Fang kidney-essence insufficiency syndrome mechanism
暂未订购 下载PDF
Jianpijiedu Fang improves survival of hepatocarcinoma mice by affecting phosphatase and tensin homolog, phosphoinositide 3-kinase, and focal adhesion kinase 认领 引用 被引量：13
17
作者 Baoguo Sun Jun Meng +5 位作者 Ting Xiang Zexiong Chen Yulong Li Lisha Lu Shijun Zhang Xiaolin Chen 《Journal of Traditional Chinese Medicine》 SCIE CSCD 2013年第4期479-485,共7页
OBJECTIVE: To investigate the effect of Jianpijiedu Fang (JPJDF) on phosphatase and tensin homolog (PTEN), phosphoinositide 3-kinase (PI3K), and focal adhesion kinase (FAK), and on the survival of hepatocellular carci... OBJECTIVE: To investigate the effect of Jianpijiedu Fang (JPJDF) on phosphatase and tensin homolog (PTEN), phosphoinositide 3-kinase (PI3K), and focal adhesion kinase (FAK), and on the survival of hepatocellular carcinoma (HCC) nude mice. METHODS: Forty male nude mice were randomly divided into 4 groups. Human HCC tissue was implanted in the livers of three groups. After 24 h, the three groups were treated respectively with JPJDF (37.5 g/kg), saline (20 mL/kg) and Tegafur (FT-207, 160 mg/kg) once a day for 10 weeks. The control group without implanting the tissue was concurrently treated with saline (20 mL/kg). The survival data and body weight of all mice were recorded, and expression levels of PTEN, PI3K and FAK in normal tissue and cancer tissue of the livers were eval-uated with immunohistochemical method. RESULTS: The cumulative survival rate of the mice in the JPJDF group was higher than those of the other groups. The rate of weight loss was the lowest in JPJDF group. The survivability and weight loss rate in FT-207 group were the poorest in all groups. The expression intensity of PTEN was higher in normal tissues than in cancer tissues, and lower in the normal tissues of HCC models than in that of mice without HCC. The PTEN expression intensity in normal tissue and cancer tissue from mice treated with FT-207 were lower than that from the mice treated with JPJDF or saline.The expression intensity of PI3K was higher in cancer tissue than in normal tissue. The PI3K expression intensity was the lowest in normal tissue and cancer tissue from mice treated with JPJDF, and the intensity from mice treated with FT-207 was the highest. In mice treated with JPJDF, the expression intensity of FAK was higher in the normal tissue and lower in the cancer tissue than those of the other treatment groups. CONCLUSION: The mechanism accounting for the prolonged survival of HCC-bearing mice treated with JPJDF might be related to the reduction in weight loss and the benign regulation of PTEN, PI3K, and FAK. 展开更多
关键词 Liver neoplasms Pten protein mouse Phosphatidylinositol 3-kinase Focal adhesion protein-tyrosine kinases Jianpijiedu Fang
暂未订购 下载PDF
Gan Shen Fu Fang ameliorates liver fibrosis in vitro and in vivo by inhibiting the inflammatory response and extracellular signalregulated kinase phosphorylation 认领 引用 被引量：4
18
作者 Qing-Hong Du Chu-Jun Zhang +8 位作者 Wei-Hong Li Yan Mu Ya Xu Scott Lowe Lin Han Xue Yu Shu-Yan Wang Yu Li Jian Li 《World Journal of Gastroenterology》 SCIE CAS 2020年第21期2810-2820,共11页
BACKGROUND Liver fibrosis is a common health problem worldwide and there is still a lack of effective medicines.The Chinese herbal medicine,Gan Shen Fu Fang(GSFF)is composed of salvianolic acid B and diammonium glycyr... BACKGROUND Liver fibrosis is a common health problem worldwide and there is still a lack of effective medicines.The Chinese herbal medicine,Gan Shen Fu Fang(GSFF)is composed of salvianolic acid B and diammonium glycyrrhizinate.In this study,we observed the effects of GSFF on liver fibrosis in vivo and in vitro in an attempt to provide some hope for the treatment.AIM To observe the effects of GSFF on liver fibrosis in vivo and in vitro and investigate the mechanism from the perspective of the inflammatory response and extracellular signal-regulated kinase(ERK)phosphorylation.METHODS Common bile duct-ligated rats were used for in vivo experiments.Hepatic stellate cells-T6(HSC-T6)cells were used for in vitro experiments.Hematoxylin and eosin staining and Masson staining,biochemical assays,hydroxyproline(Hyp)assays,enzyme-linked immunoasorbent assay and western blotting were performed to evaluate the degree of liver fibrosis,liver function,the inflammatory response and ERK phosphorylation.The CCK8 assay,immunofluorescence and western blotting were applied to test the effect of GSFF on HSC-T6 cell activation and determine whether GSFF had an effect on ERK phosphorylation in HSC-T6 cells.RESULTS GSFF improved liver function and inhibited liver fibrosis in common bile ductligated rats after 3 wk of treatment,as demonstrated by histological changes,hydroxyproline assays and collagen I concentrations.GSFF alleviated inflammatory cell infiltration and reduced the synthesis of pro-inflammatory cytokines[tumor necrosis factor-α(TNF-α)and interlukin-1β]and NF-κB.In addition,GSFF decreased ERK phosphorylation.In vitro,GSFF inhibited the viability of HSC-T6 cells with and without transforming growth factorβ1(TGF-β1)stimulation and decreased the synthesis of collagen I.GSFF had the greatest effect at a concentration of 0.5μmol/L.GSFF inhibited the expression ofα-smooth muscle actin(α-SMA),a marker of HSC activation,in HSC-T6 cells.Consistent with the in vivo results,GSFF also inhibited the phosphorylation of ERK and downregulated the expression of NF-κB.CONCLUSION GSFF inhibited liver fibrosis progression in vivo and HSC-T6 cell activation in vitro.These effects may be related to an alleviated inflammatory response and downregulated ERK phosphorylation. 展开更多
关键词 Liver fibrosis Gan Shen Fu Fang Inflammatory response Extracellular signal-regulated kinase phosphorylation In vivo In vitro
暂未订购 下载PDF
Efficacy of Dangua Fang(丹瓜方) on endothelial cells damaged by oxidative stress 认领 引用 被引量：4
19
作者 HENG Xianpei LI Liang +1 位作者 YANG Liuqin WANG Zhita 《Journal of Traditional Chinese Medicine》 SCIE CSCD 2022年第6期900-907,共8页
OBJECTIVE: To evaluate the protective effects of serum containing Dangua Fang( 丹 瓜 方) on vascular endothelium damaged by oxidative stress. METHODS: Five experiments were completed in this paper. In the first experi... OBJECTIVE: To evaluate the protective effects of serum containing Dangua Fang( 丹 瓜 方) on vascular endothelium damaged by oxidative stress. METHODS: Five experiments were completed in this paper. In the first experiment, we found the most suitable serum containing Dangua Fang by comparing groups with different serum containing Dangua Fang. In the second experiments we analyzed Dangua Fang influencing endothelial cell viability and apoptosis and cell cycle. The third experiment on Dangua Fang intervention of mitochondrial respiratory chain. The fourth experiment on Dangua Fang intervention of mitochondrial membrane potential. And finally, on the fifth experiment we researched the mechanism of Dangua Fang improving mitochondrial function by comparing the Na~+-k~+-ATPase and peroxisome proliferator-activated receptor-gamma coactivator-1alpha(PGC-1α) in the Dangua group with the diazoxide group and Co Q+Vit C group. RESULTS: We compared the control group in the first experiments and the OD values in DZ1 group was the most significant in all intervening groups. The recipe of DZ1(5% serum containing Dangua Fang) was used in the following experiments. Compared with the control group, cell viability, cell cycle(G2 + S), cytochrome c oxidase(COX), R3 red/green, R2 red/green, R1 red/green decreased and apoptosis, succinate dehydrogenase(SDH), green(R2 + R3), Na+-k+-ATPase, PGC-1α increased in the model group. Compared with the model group, cell viability, G2+S, COX, R3 red/green, R2 red/green, R1 red/green raised and apoptosis, green(R2 + R3), Na-K-ATPase decreased in the Dangua group;G2 + S, R3 red/green, R2 red/green, R1 red/green raised and green(R2 + R3) decreased in the Co Q + Vit C group. Na-K-ATPase increased in the combined group(P < 0.05 or < 0.01). CONCLUSIONS: Dangua Fang protects oxidative stressinduced endothelial cells damaged by promotion of mitochondrial biogenesis, reduction of Na~+-K~+-ATPase activity and regulation of mitochondrial respiratory chain function restoring mitochondrial membrane potential. 展开更多
关键词 mitochondria oxidative stress endothelial cells peroxisome proliferator-activated receptor gamma coactivator 1-alpha Dangua Fang
暂未订购 下载PDF
Mechanism of anti-arterial thrombosis of Dahuangzhecong Fang screened by Ti-Al intermetallic compound porous material 认领 引用 被引量：2
20
作者 万玲 王战义 +6 位作者 魏星 李佶阳 钟广伟 段晓鹏 贺福元 江垚 王东生 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2012年第12期3156-3160,共5页
The mechanism of antithrombotic of Dahuangzhechong Fang separated and purified by Ti-Al intermetallic compound porous material (TAICPM) was researched. Dahuangzhechong Fang, which was isolated and screened by TAICPM, ... The mechanism of antithrombotic of Dahuangzhechong Fang separated and purified by Ti-Al intermetallic compound porous material (TAICPM) was researched. Dahuangzhechong Fang, which was isolated and screened by TAICPM, was used to oral rats. At the end of study, their blood and thrombus were collected. The results show that TAICPM with the pore size of 1-5 μm can screen Dahuangzhechong Fang well. Dahuangzhechong Fang can increase 6-keto-PGF1α, lower content of TXD2 and platelet. Dahuangzhechong Fang has good effect to resist arterial thrombosis. 展开更多
关键词 Ti-Al intermetallic compound porous materials Dahuangzhecong Fang arterial thrombosis isolated Chinese medicine
暂未订购 下载PDF
已选0条
引用分析
参考文献
引证文献
 统计分析
检索结果
已选文献
上一页 1 2 … 218 下一页 到第 页
在线咨询 使用帮助 返回顶部 意见反馈