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Higher-Order Expansions of Powered Order Statistics of Maxwell Sequences 认领 引用
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作者 HUANG Jian-wen LIU Xin-ling +1 位作者 JIA Jin-ping WANG Run-ke 《Chinese Quarterly Journal of Mathematics》 2026年第2期162-173,共12页
Under the optimal norming constants, this paper studies the higher-order expansions of the distributions and densities of the powered order statistics of Maxwell sequence. As auxiliary results, the corresponding conve... Under the optimal norming constants, this paper studies the higher-order expansions of the distributions and densities of the powered order statistics of Maxwell sequence. As auxiliary results, the corresponding convergence rates are obtained. The results show that the convergence rates of distributions and densities of normalized power order statistics are related to power index in principle. Finally, we compared the accuracy of each approximations with its true values through numerical experiments. 展开更多
关键词 Maxwell sequences Powered order statistics Higher-order expansion Convergence rate
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Genetic diversity and population structure of the Fujian oyster Crassostrea angulata revealed by mitochondrial COI gene and nuclear gene ITS2 sequences 认领 引用
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作者 Shiqi YU Ziqiang HAN Huayong QUE 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2026年第1期419-432,共14页
The Fujian oyster(Crassostrea angulata) is an economically significant shellfish species distributed mainly along the Fujian coast, Southeast China. However, its genetic diversity and structure remain unclear. The mai... The Fujian oyster(Crassostrea angulata) is an economically significant shellfish species distributed mainly along the Fujian coast, Southeast China. However, its genetic diversity and structure remain unclear. The main distribution area of the C. angulata is located in Fujian, South China. In total, 420 C. angulata were collected from 14 natural habitats(populations) along the Fujian coast, and their genetic diversity and structure were analyzed in the mitochondrial COI and nuclear gene ITS2 sequences. Results reveal that all the 14 populations of C. angulata exhibited high levels of genetic diversity, with a total of 57(haplotype diversity: 0.811±0.016) and 124(haplotype diversity: 0.912±0.007) haplotypes revealed by COI and ITS2, respectively. Notably, significant intermediate level of genetic differentiations between the Ningde Zhujiang(ZJ) population(FS T by COI: 0.035–0.142, P<0.05;FS T by ITS2: 0.078–0.123, P<0.05) with other populations were observed for the first time, which is also supported by the results of molecular variance analysis(FC T by COI: 0.105, P<0.05;FC T by ITS2: 0.086, P<0.05) and the clustering of the ZJ population into distinct branches in the interpopulation genetic differentiation tree. Furthermore, the evolutionary tree and haplotype network analyses do not support the formation of a clear geographical genealogical structure among these 14 populations. In addition, the population dynamics analysis suggests that the C. angulata may have undergone expansion during the third ice age of the Pleistocene. These results provide a reference for the preservation and further genetic improvement of C. angulata. 展开更多
关键词 Crassostrea angulata genetic diversity population structure mitochondrial COⅠgene nuclear gene ITS2 sequence
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Seismic Response of Offshore Reinforced Concrete Bridges Under Main-Aftershock Sequences 认领 引用
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作者 CAO Jing LI Yue +1 位作者 ZHANG Changyong LI Chong 《吉首大学学报（自然科学版）》 CAS 2026年第2期62-71,共10页
To investigate the seismic response evolution of offshore bridges under the coupled effects of main-aftershock sequences and chloride-induced erosion,a time-dependent damage finite element model of a three-span,three-... To investigate the seismic response evolution of offshore bridges under the coupled effects of main-aftershock sequences and chloride-induced erosion,a time-dependent damage finite element model of a three-span,three-column simply supported bridge was established based on energy dissipation theory and static pushover analysis.Seismic time-history analysis was conducted to obtain key response indicators,including the maximum displacement and residual displacement at the pier top,as well as the proportion of cumulative hysteretic energy generated at the pier bottom during aftershocks.The results show that structural durability degradation significantly affected the seismic responses:under the same seismic excitation,the maximum pier-top displacement of long-term service bridges increased by at least 10%,with a maximum increase of up to 43%,compared with newly built bridges.When subjected to high-intensity earthquakes,the residual displacement at the pier top continuously increased with service time,rising by 66%,121%,and 163%after 50,75,and 100 a of service,respectively,compared with new bridges.Aftershocks induced noticeable cumulative damage at critical locations such as the pier bottom,with the cumulative hysteretic energy accounting for an average of 14.08%of the total energy dissipation during the main-aftershock sequence,reaching a maximum of 25.3%.Therefore,it is recommended that both the energy contribution of aftershocks and the effect of durability degradation be considered in the seismic design of offshore bridges. 展开更多
关键词 main-aftershock sequences chloride ion erosion simply-supported bridge maximum displacement residual displacement hysteresis curve
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Effects of welding sequences and filler metals on microstructure and mechanical properties of N08825/L360 nickel-based alloy clad plate welded joints 认领 引用
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作者 ZHU Min 《Baosteel Technical Research》 CAS 2026年第2期33-46,共14页
Nickel-based alloy clad plate(NACP)is a promising composite structural material owing to its excellent mechanical and corrosion-resistant properties,as well as its low cost.However,the development of welding technolog... Nickel-based alloy clad plate(NACP)is a promising composite structural material owing to its excellent mechanical and corrosion-resistant properties,as well as its low cost.However,the development of welding technology for NACP has lagged behind.Herein,N08825/L360 NACP was used as the model material,and welded joints were prepared using tungsten inert gas welding to investigate the effects of different welding sequences and filler metals on the micro structure and mechanical properties of joints.The results showed that weld seams filled with mixed filler metals under the post-flyer welding seam welding sequence exhibited a uniform micro structure,and the joints performed well in tensile,bending,and impact tests.For joints filled with mixed filler metals under the post-base welding seam sequence(P-BWS)satisfactory face-bending,root-bending,and tensile properties were achieved;however,cracks formed in the side-bending specimens,and the impact toughness decreased greatly.Micro structural analysis shows that the deterioration in weld toughness is attributable to two factors:severe dimensional embrittlement and micro structural embrittlement,both caused by the formation of hardened welds in the welding seam.Under the P-BWS,using only nickel-based alloy filler metals effectively prevents the deterioration of the micro structure and properties of welding seams. 展开更多
关键词 N08825/L360 nickel-based alloy clad plate welding sequence welding filler metals microstructure mechanical property
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基于Fibonacci-Lucas序列的8环QC-LDPC码新颖构造方法 认领 引用
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作者 袁建国 伏博文 +1 位作者 杨婷 胡坤 《半导体光电》 CAS 北大核心 2025年第3期543-549,共7页
针对当前准循环低密度奇偶校验(Quasi-Cyclic Low-Density Parity-Check,QCLDPC)码存在短环结构并且其纠错性能差的问题,文章基于斐波那契-卢卡斯序列(FibonacciLucas Sequence,FLS)提出一种围长至少为8的QC-LDPC码新颖构造方法。该方... 针对当前准循环低密度奇偶校验(Quasi-Cyclic Low-Density Parity-Check,QCLDPC)码存在短环结构并且其纠错性能差的问题,文章基于斐波那契-卢卡斯序列(FibonacciLucas Sequence,FLS)提出一种围长至少为8的QC-LDPC码新颖构造方法。该方法首先利用Fibonacci-Lucas序列构成一个满足无4环条件的指数矩阵,再利用搜索算法搜索出让该指数矩阵满足无6环条件的元素得到一个递增序列,构造相应的指数矩阵,得到其奇偶校验矩阵。仿真结果表明,当误码率为1×10-6时,所构造的FLS-QC-LDPC码相较于其他三种具有相同码率和码长的QC-LDPC码,其净编码增益得到了明显改善,表现出较好的纠错性能。此外,该构造方法灵活多变,支持多种码长和码率选择,同时保持较低的计算复杂度,高效且适应性强。 展开更多
关键词 准循环低密度奇偶校验码 Fibonacci-Lucas序列 搜索算法 净编码增益
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High efficiency of thalassemia prevention by next-generation sequencing:a real-world cohort study in two centers of China 认领 引用 被引量：3
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作者 Jinman Zhang Wenqian Zhang +18 位作者 Haoqing Zhang Aiqi Cai Caiyun Li Ling Liu Jufang Tan Yang Yang Wen Yuan Jing He Shiping Chen Yingli Cao Yan Zhang Jie Zhang Rui Zhou Shuai Hou Dongqun Huang Danjing Chen Zhiyu Peng Dongzhu Lei Baosheng Zhu 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2026年第1期87-96,共10页
The occurrence of severe thalassemia,an inherited blood disorder that is either blood-transfusiondependent or fatal,can be mitigated through carrier screening.Here,we aim to evaluate the effectiveness and outcomes of ... The occurrence of severe thalassemia,an inherited blood disorder that is either blood-transfusiondependent or fatal,can be mitigated through carrier screening.Here,we aim to evaluate the effectiveness and outcomes of pre-conceptional and early pregnancy screening initiatives for severe thalassemia prevention in a diverse population of 28,043 women.Using next-generation sequencing(NGS),we identify 4,226(15.07%)thalassemia carriers across 29 ethnic groups and categorize them into high-(0.75%),low-(25.86%),and unknown-risk(69.19%)groups based on their spouses'screening results.Post-screening follow-up reveals 59 fetuses with severe thalassemia exclusively in high-risk couples,underscoring the efficacy of risk classification.Among 25,053 live births over 6 months of age,two severe thalassemia infants were born to unknown-risk couples,which was attributed to incomplete screening and late NGS-based testing for a rare variant.Notably,64 rare variants are identified in 287 individuals,highlighting the genetic heterogeneity of thalassemia.We also observe that migrant flow significantly impacts carrier rates,with 93.90%of migrants to Chenzhou originating from high-prevalence regions in southern China.Our study demonstrates that NGS-based screening during pre-conception and early pregnancy is effective for severe thalassemia prevention,emphasizing the need for continuous screening efforts in areas with high and underestimated prevalence. 展开更多
关键词 Thalassemia Carrier screening Next generation sequencing Rare thalassemia Clinical effectiveness Blood-transfusion-dependent
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Changes in border-associated macrophages after stroke: Single-cell sequencing analysis 认领 引用 被引量：2
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作者 Ning Yu Yang Zhao +3 位作者 Peng Wang Fuqiang Zhang Cuili Wen Shilei Wang 《Neural Regeneration Research》 SCIE CAS CSCD 2026年第1期346-356,共11页
Border-associated macrophages are located at the interface between the brain and the periphery, including the perivascular spaces, choroid plexus, and meninges. Until recently, the functions of border-associated macro... Border-associated macrophages are located at the interface between the brain and the periphery, including the perivascular spaces, choroid plexus, and meninges. Until recently, the functions of border-associated macrophages have been poorly understood and largely overlooked. However, a recent study reported that border-associated macrophages participate in stroke-induced inflammation, although many details and the underlying mechanisms remain unclear. In this study, we performed a comprehensive single-cell analysis of mouse border-associated macrophages using sequencing data obtained from the Gene Expression Omnibus(GEO) database(GSE174574 and GSE225948). Differentially expressed genes were identified, and enrichment analysis was performed to identify the transcription profile of border-associated macrophages. CellChat analysis was conducted to determine the cell communication network of border-associated macrophages. Transcription factors were predicted using the ‘pySCENIC' tool. We found that, in response to hypoxia, borderassociated macrophages underwent dynamic transcriptional changes and participated in the regulation of inflammatory-related pathways. Notably, the tumor necrosis factor pathway was activated by border-associated macrophages following ischemic stroke. The pySCENIC analysis indicated that the activity of signal transducer and activator of transcription 3(Stat3) was obviously upregulated in stroke, suggesting that Stat3 inhibition may be a promising strategy for treating border-associated macrophages-induced neuroinflammation. Finally, we constructed an animal model to investigate the effects of border-associated macrophages depletion following a stroke. Treatment with liposomes containing clodronate significantly reduced infarct volume in the animals and improved neurological scores compared with untreated animals. Taken together, our results demonstrate comprehensive changes in border-associated macrophages following a stroke, providing a theoretical basis for targeting border-associated macrophages-induced neuroinflammation in stroke treatment. 展开更多
关键词 border-associated macrophages clodronate hypoxia ischemia-reperfusion ischemic stroke liposomes neuroinflammation single-cell sequencing analysis STAT3 tumor necrosis factor
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Blood-brain barrier disruption and neuroinflammation in the hippocampus of a cardiac arrest porcine model:Single-cell RNA sequencing analysis 认领 引用 被引量：1
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作者 Tangxing Jiang Yaning Li +11 位作者 Hehui Liu Yijun Sun Huidan Zhang Qirui Zhang Shuyao Tang Xu Niu Han Du Yinxia Yu Hongwei Yue Yunyun Guo Yuguo Chen Feng Xu 《Neural Regeneration Research》 SCIE CAS CSCD 2026年第2期742-755,共14页
Global brain ischemia and neurological deficit are consequences of cardiac arrest that lead to high mortality.Despite advancements in resuscitation science,our limited understanding of the cellular and molecular mecha... Global brain ischemia and neurological deficit are consequences of cardiac arrest that lead to high mortality.Despite advancements in resuscitation science,our limited understanding of the cellular and molecular mechanisms underlying post-cardiac arrest brain injury have hindered the development of effective neuroprotective strategies.Previous studies primarily focused on neuronal death,potentially overlooking the contributions of non-neuronal cells and intercellular communication to the pathophysiology of cardiac arrest-induced brain injury.To address these gaps,we hypothesized that single-cell transcriptomic analysis could uncover previously unidentified cellular subpopulations,altered cell communication networks,and novel molecular mechanisms involved in post-cardiac arrest brain injury.In this study,we performed a single-cell transcriptomic analysis of the hippocampus from pigs with ventricular fibrillation-induced cardiac arrest at 6 and 24 hours following the return of spontaneous circulation,and from sham control pigs.Sequencing results revealed changes in the proportions of different cell types,suggesting post-arrest disruption in the blood-brain barrier and infiltration of neutrophils.These results were validated through western blotting,quantitative reverse transcription-polymerase chain reaction,and immunofluorescence staining.We also identified and validated a unique subcluster of activated microglia with high expression of S100A8,which increased over time following cardiac arrest.This subcluster simultaneously exhibited significant M1/M2 polarization and expressed key functional genes related to chemokines and interleukins.Additionally,we revealed the post-cardiac arrest dysfunction of oligodendrocytes and the differentiation of oligodendrocyte precursor cells into oligodendrocytes.Cell communication analysis identified enhanced post-cardiac arrest communication between neutrophils and microglia that was mediated by neutrophil-derived resistin,driving pro-inflammatory microglial polarization.Our findings provide a comprehensive single-cell map of the post-cardiac arrest hippocampus,offering potential novel targets for neuroprotection and repair following cardiac arrest. 展开更多
关键词 Blood-brain barrier disruption cardiac arrest hippocampus microglia neuroinflammation neuroprotection neutrophil oligodendrocyte dysfunction S100A8 single-cell RNA sequencing
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Non-Abelian Extensions of Rota-Baxter Pre-Lie Algebras and Wells Exact Sequences 认领 引用
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作者 Shuangjian GUO Qun WANG 《Journal of Mathematical Research with Applications》 CSCD 2025年第4期473-487,共15页
In this paper,we introduce non-abelian cohomology groups and classify the nonabelian extensions of Rota-Baxter pre-Lie algebras in terms of non-abelian cohomology groups.Next,we explore the inducibility of pairs of au... In this paper,we introduce non-abelian cohomology groups and classify the nonabelian extensions of Rota-Baxter pre-Lie algebras in terms of non-abelian cohomology groups.Next,we explore the inducibility of pairs of automorphisms and derive the analog Wells exact sequences under the circumstance of Rota-Baxter pre-Lie algebras.Finally,we discuss the inducibility problem of pairs of automorphisms about an abelian extensions of Rota-Baxter pre-Lie algebras. 展开更多
关键词 Rota-Baxter pre-Lie algebra non-abelian extension Wells exact sequences
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Single-nucleus RNA sequencing reveals transcriptional response of wheat roots and leaves to salt stress 认领 引用
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作者 Linyi Qiao Hong Liu +9 位作者 Tian Li Yiming Ma Jingjing Ning Zhijian Chang Junming Li Xigang Liu Zhiyong Zhao Xingwei Zheng Jiajia Zhao Jun Zheng 《The Crop Journal》 SCIE CSCD 2026年第4期1181-1192,共12页
Deeper understanding of the mechanism by which wheat responds to salt stress(SS)remains a major challenge due to the scarcity of available single-cellucleus transcriptomics resources.Here,in order to uncover the trans... Deeper understanding of the mechanism by which wheat responds to salt stress(SS)remains a major challenge due to the scarcity of available single-cellucleus transcriptomics resources.Here,in order to uncover the transcriptional patterns during the late stage of SS of different wheat cells,we performed single-nucleus RNA-sequencing(snRNA-seq)on roots and leaves of wheat seedlings under NaCl treatment for 7 d.Integrating snRNA-seq with bulk RNA-seq and physiological and biochemical indices measurement,the single-cell transcriptome atlas of wheat roots and leaves was constructed,and response patterns of cell types to SS were identified based on enrichment of differentially expressed genes for osmotic stress,ion transport,and oxidative stress.Moreover,several cell-type-specific salt-tolerant candidate genes were determined based on pseudotime analysis and functional validation,such as TaWRKY75-A in root hair cells,NICOTIANAMINE SYNTHASE(NAS)genes in root stele I cells,and dehydrin(DHN)genes in leaf fiber cells. 展开更多
关键词 Wheat Single-nucleus RNA sequencing Roots Leaves Salt stress
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Identification of gene variants in 30 patients from southeastern China with severe hypospadias by whole-exome sequencing 认领 引用
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作者 Wen-Hua Huang Qian-Qian Tan +6 位作者 Wei Zeng Hai-Gen Wang Xun Cui En-Hui Wang Yong Zhou Wen-Hao Ni Chao-Ming Zhou 《Asian Journal of Andrology》 SCIE CAS CSCD 2026年第3期276-283,共8页
Hypospadias is a common congenital malformation of the male external genitalia, with severe cases presenting considerable surgical and long-term challenges. Despite the clinical importance of severe hypospadias demons... Hypospadias is a common congenital malformation of the male external genitalia, with severe cases presenting considerable surgical and long-term challenges. Despite the clinical importance of severe hypospadias demonstrated by prolonged hospital stays, repeated surgeries, and substantial costs, the genetic etiology of severe hypospadias remains incompletely understood, particularly in diverse populations. To determine the molecular basis, we performed whole-exome sequencing (WES) on 30 Chinese patients from southeastern China with confirmed 46,XY karyotypes. Our analysis identified clinically relevant genetic variants, including single-nucleotide variants (SNVs) and copy number variations (CNVs), with subsequent phenotypic correlation. Clinically relevant genetic variants were identified in 33.3% (10/30) of cases, including novel SNVs in gonadal regulators (nuclear receptor subfamily 5 group A member 1 [NR5A1] c.1344dupC/c.244+1G>T and SRY-box 3 [SOX3] c.1273G>C), morphogenetic modulators (GLI family zinc finger 3 [GLI3] c.4731delA and aristaless-related homeobox [mARX] c.644C>G), and syndromic genes (patched domain containing 1 [PTCHD1] c.667G>A and euchromatic histone lysine methyltransferase 1 [EHMT1] c.3081C>T). Additionally, recurrent CNVs at 22q12.3 and a novel CNV exon 18 deletion in myelin regulatory factor (MYRF) and 18q11.2 were identified. Mutation carriers showed a significantly higher frequency of cryptorchidism (40.0% vs 5.0%, P < 0.01) and a higher prevalence of ≥3 associated malformations (80.0% vs 35.0%, P < 0.05) than non-carriers, highlighting genotype–phenotype correlations. The 33.3% diagnostic yield tripled conventional estimates, demonstrating WES efficacy in identifying SNVs and CNVs in severe phenotypes. These findings reveal the genetic heterogeneity of severe hypospadias and support WES utility in uncovering novel variants and structural genomic alterations. 展开更多
关键词 copy number variations genotype-phenotype correlations hypospadias whole-exome sequencing
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Whole genome sequencing analysis reveals strong reproductive isolation between two hybridizing Rhododendron species in subgenus Tsutsusi 认领 引用
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作者 Xiaoling Tian Ningning Zhang +5 位作者 Xiaohua Li Zhong Zhang Heng Shu Chunying Zhang Yongpeng Ma Yupeng Geng 《Plant Diversity》 SCIE CAS CSCD 2026年第1期212-215,共4页
Natural hybridization is known to play a vital role in speciation;however,the mechanisms underlying the early stages of natural hybridization remain unclear.Where two plant species come into contact,two driving forces... Natural hybridization is known to play a vital role in speciation;however,the mechanisms underlying the early stages of natural hybridization remain unclear.Where two plant species come into contact,two driving forces may balance the dynamic consequences of hybridization:fusion by hybridization-mediated gene flow,and separation by reproductive isolation(RI)(Ma et al.,2010a,b;Chang et al.,2022). 展开更多
关键词 Reproductive isolation Natural hybridization Rhododendron Mutation load Whole genome sequence
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Constructions of Control Sequence Set for Hierarchical Access in Data Link Network 认领 引用
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作者 Niu Xianhua Ma Jiabei +3 位作者 Zhou Enzhi Wang Yaoxuan Zeng Bosen Li Zhiping 《China Communications》 SCIE EI CSCD 2026年第1期67-80,共14页
As an important resource in data link,time slots should be strategically allocated to enhance transmission efficiency and resist eavesdropping,especially considering the tremendous increase in the number of nodes and ... As an important resource in data link,time slots should be strategically allocated to enhance transmission efficiency and resist eavesdropping,especially considering the tremendous increase in the number of nodes and diverse communication needs.It is crucial to design control sequences with robust randomness and conflict-freeness to properly address differentiated access control in data link.In this paper,we propose a hierarchical access control scheme based on control sequences to achieve high utilization of time slots and differentiated access control.A theoretical bound of the hierarchical control sequence set is derived to characterize the constraints on the parameters of the sequence set.Moreover,two classes of optimal hierarchical control sequence sets satisfying the theoretical bound are constructed,both of which enable the scheme to achieve maximum utilization of time slots.Compared with the fixed time slot allocation scheme,our scheme reduces the symbol error rate by up to 9%,which indicates a significant improvement in anti-interference and eavesdropping capabilities. 展开更多
关键词 control sequence data link hierarchical access control theoretical bound
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Single-Nucleus Transcriptomic Sequencing Revealed Cellular and Molecular Changes in a Pilocarpine-Induced Epilepsy Rat Model 认领 引用
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作者 Ying Wang Yue Wang +3 位作者 Fei Yu Yidi Liu Xin Liu Zhengxu Cai 《Neuroscience Bulletin》 SCIE CAS CSCD 2026年第3期539-558,共20页
Uncovering the underlying process of epileptogenesis is crucial for developing effective treatment strategies for epilepsy.However,the cellular and molecular changes throughout epileptogenesis are not fully understood... Uncovering the underlying process of epileptogenesis is crucial for developing effective treatment strategies for epilepsy.However,the cellular and molecular changes throughout epileptogenesis are not fully understood.In this study,single-nucleus RNA sequencing was performed on the hippocampus,temporal cortex,and thalamus across the acute,latent,and chronic phases in a pilocarpine-induced rat model and controls.We created a comprehensive single-nucleus transcriptomic atlas of rat epileptogenesis,consisting of 311,177 single nuclei.Our analysis revealed distinct transcriptional signatures across the three phases and regions,including significant gene expression changes in the acute phase and critical synaptic and neural network remodeling in the thalamus during the latent phase.Notably,we identified two novel astrocyte clusters during epileptogenesis,with the EX-Astro C3-IN pathway emerging as a potential intervention target.The dataset provides a detailed understanding of the dynamic cellular and molecular landscape of epileptogenesis. 展开更多
关键词 Epileptogenesis Single-nucleus RNA sequencing Pilocarpine-induced epilepsy rat model Latent phase Astrocytes
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Whole-genome sequencing reveals Yunnan as the crossroads of east and Southeast Asia for human gene flow 认领 引用
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作者 Xiaobo Qian Bo Li +15 位作者 Jianmei Liu Yushan Huang Wenxi Gu Yuwen Zhou Qiong Nan Chao Wang Le Cheng Junkun Niu Fengrui Zhang Qian Li Xiuqing Zhang Jinlong Yang Yinglei Miao Mingyan Fang Xin Jin Yang Sun 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2026年第5期810-826,共17页
Yunnan Province has long served as a key nexus facilitating economic and cultural exchanges between East Asia,Southeast Asia,and Qinghai-Xizang Plateau.However,previous genetic studies were largely limited by sparse m... Yunnan Province has long served as a key nexus facilitating economic and cultural exchanges between East Asia,Southeast Asia,and Qinghai-Xizang Plateau.However,previous genetic studies were largely limited by sparse marker density,low sequencing depth,or single-population designs,leaving the population genetic structure and demographic history insufficiently resolved.Here,we conduct a high-resolution population genetic study based on 366 high-depth whole-genome sequencing samples from 6 ethnic groups,including Bai,Dai,Hani,Miao,Tibetan,and Han.We identify approximately 3.51 million novel variants and reveal fine-scale population structure and complex demographic histories among Yunnan ethnic groups.Beyond the three ancestries proposed by the tri-genealogy hypothesis,we detect a Han Chinese-related lineage,Yan-Huang,within multiple Yunnan populations.We further demonstrate that Yunnan represents a major gene-flow hotspot across East and Southeast Asia despite geographic barriers.Finally,we identify genomic loci under positive selection with candidate genes enriched in immune regulation,energy metabolism,cardiac development,and dietary adaptation,highlighting the role of local environmental pressures in shaping the genetic diversity of Yunnan populations. 展开更多
关键词 Yunnan populations Whole-genome sequencing Population structure Demographic history Gene flow Positive selection
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ERBB2 mutations promote recurrence and metastasis in non-muscle-invasive bladder cancer via HIF-1 phosphorylation:Insights from whole exome sequencing 认领 引用
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作者 Xu Wang Long Jin +3 位作者 Xinlin Zou Ankang Zhu Mingyu Li Haitao Fan 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2026年第1期281-298,共18页
This study investigates the role of ERBB2 mutations in promoting recurrence and metastasis of non-muscle-invasive bladder cancer(NMIBC).Analysis of whole exome sequencing(WES)data from The Cancer Genome Atlas(TCGA)and... This study investigates the role of ERBB2 mutations in promoting recurrence and metastasis of non-muscle-invasive bladder cancer(NMIBC).Analysis of whole exome sequencing(WES)data from The Cancer Genome Atlas(TCGA)and the International Cancer Genome Consortium(ICGC)databases revealed a significant association between ERBB2 mutations and immune cell infiltration.To validate these findings,formalin-fixed,paraffin-embedded tumor tissues from patients with recurrent NMIBC were analyzed,with a focus on ERBB2 mutations.In addition,bladder cancer cell lines carrying wild type or mutant ERBB2 were established using clustered regularly interspaced short palindromic repeats(CRISPR)-associated protein 9(CRISPR/Cas9)technology.Functional experiments,including Western blotting,protein stability assays,and ubiquitination analyses,demonstrated that ERBB2 mutations promote hypoxia-inducible factor-1(HIF-1)phosphorylation,leading to its stabilization and enhancing the proliferative,migratory,and invasive capacities of tumor cells.Furthermore,flow cytometry,5-ethynyl-2′-deoxyuridine(EdU),Cell Counting Kit-8(CCK-8),and Transwell assays confirmed the impact of these mutations on cellular behavior,while drug sensitivity assays indicated increased susceptibility of ERBB2-mutant cells to therapeutic agents.In vivo studies using mouse models further supported these findings,showing that ERBB2 mutations promote tumor growth,metastasis,and macrophage infiltration.Collectively,these results suggest that ERBB2 mutations drive NMIBC progression by stabilizing HIF-1 through phosphorylation,thereby facilitating tumor development and immune modulation,and underscore the potential of ERBB2 as a therapeutic target for preventing NMIBC recurrence and metastasis. 展开更多
关键词 Whole exome sequencing Non-muscle-invasive bladder cancer Bioinformatics ERBB2 HIF-1 Phosphorylation Proliferation Metastasis
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Sedimentary Characteristics and Evolution of the High-resolution Sequence Stratigraphic Framework of the Taiyuan Formation in the Hangjinqi Area,Northern Ordos Basin 认领 引用
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作者 ZHAO Mingsheng TIAN Jingchun +1 位作者 SU Bingrui ZHANG Xiang 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2026年第1期54-68,共15页
The Ordos Basin is a large superimposed hydrocarbon-bearing basin in China,and further research on the sedimentary characteristics and sedimentary evolution of the sequence framework of target layers is of great theor... The Ordos Basin is a large superimposed hydrocarbon-bearing basin in China,and further research on the sedimentary characteristics and sedimentary evolution of the sequence framework of target layers is of great theoretical and practical significance for guiding oil and gas exploration.The sedimentary facies and sedimentary evolution of the high-resolution sequence framework of the Carboniferous Taiyuan Formation in the Hangjinqi area have been systematically analyzed for the first time by drilling,logging and seismic data.The results show that four types of sequence interfaces can be identified in the Taiyuan Formation:regional unconformity surfaces,scour surfaces,lithologic-lithofacies transformation surfaces and flooding surfaces.According to the sedimentary response caused by the upward and downward movements of the base level at different levels,the Taiyuan Formation can be divided into 2 long-term cycles(LSC1-LSC2),4 mid-term cycles(MSC1-MSC4)and 7 short-term cycles(SSC1-SSC7).The long-and mid-term cycles correspond to members T1and T2and layers T1-1,T1-2,T2-1,and T2-2,respectively.Long-term cycles are dominated by C1;mid-term cycles are dominated by C1and C2,followed by A2;and short-term cycles are dominated by C1,C2,A1 and A2.Under the high-resolution sequence stratigraphic framework,the Hangjinqi area underwent a transformation of fan delta and tidal flat depositional systems during the Taiyuan Formation sedimentary period.In the MSC1-MSC2stage,owing to a large-scale paleocontinent,the fan delta sedimentary body,which was limited in scale and scope,developed only in the southeastern corner and gradually transitioned basinward to tidal flat facies.In the MSC3-MSC4 stage,as the paleocontinent continuously decreased and the sedimentary range expanded,fan-delta plain sedimentation began in the study area.Several braided distributary channels with poor connectivity developed on the fan-delta plain,and between them were floodplains and peat swamps. 展开更多
关键词 high-resolution sequence framework sedimentary characteristics sedimentary evolution Taiyuan Formation Hangjinqi area northern Ordos Basin
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Integration of Single-cell RNA Sequencing and Mendelian Randomization Analysis for Identifying Potential Immune Therapeutic Targets in Amyotrophic Lateral Sclerosis 认领 引用
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作者 Xinyuan Pang Hongfen Wang +1 位作者 Jiongming Bai Xusheng Huang 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2026年第3期327-341,共15页
Objective Adaptive immune responses play a critical role in the pathogenesis of amyotrophic lateral sclerosis(ALS).In this study,we investigated the functional mechanisms of T cell subtypes and assessed the causal lin... Objective Adaptive immune responses play a critical role in the pathogenesis of amyotrophic lateral sclerosis(ALS).In this study,we investigated the functional mechanisms of T cell subtypes and assessed the causal links between CD4+cytotoxic T cell-related genes and ALS risk.Methods Single-cell RNA sequencing(scRNA-seq)of peripheral blood mononuclear cells(PBMCs)from patients with ALS and healthy controls(HC)was used to identify differentially expressed genes(DEGs)in CD4+cytotoxic T cells.Comprehensive analyses of CD4+cytotoxic T cells,including pseudotemporal trajectory,intercellular communication,and metabolic pathway analysis,were performed.Mendelian randomization(MR)analysis evaluated the causal effects of DEGs on ALS risk,with validation using independent genome-wide association study(GWAS)data.Expression patterns of the causal genes were further verified using scRNA-seq,bulk-seq,and clinical samples.Results CD4+cytotoxic T cells were significantly expanded in patients with ALS.The upregulated genes S100A6,SERPINB6,SMAD7,and TPST2 were positively correlated with ALS susceptibility,whereas DIP2A showed a protective association.Conclusion S100A6,SERPINB6,SMAD7,TPST2,and DIP2A were identified as causal genes and potential therapeutic targets in ALS,implicating CD4+cytotoxic T cells in the disease mechanisms.Further studies targeting these genes and neuroinflammatory pathways are warranted. 展开更多
关键词 Amyotrophic lateral sclerosis CD4+cytotoxic T cells Drug target Mendelian randomization Single-cell RNA sequencing
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A case report of brain abscess caused by Nocardia cyriacigeorgica identified by metagenomic next-generation sequencing 认领 引用
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作者 Wenqing Tu Peiting Zeng +5 位作者 Zhuangtian Wu Zuyong Li Tao Yu Wenxian Zhang Ran Chen Lian Liang 《World Journal of Emergency Medicine》 SCIE CAS CSCD 2026年第2期199-201,共3页
Nocardia is an aerobic,gram-positive,and opportunistic bacillus widely distributed in the environment.Nocardia cyriacigeorgica(N.cyriacigeorgica) was first isolated in 2001 from a chronic bronchitis patient,[1] and... Nocardia is an aerobic,gram-positive,and opportunistic bacillus widely distributed in the environment.Nocardia cyriacigeorgica(N.cyriacigeorgica) was first isolated in 2001 from a chronic bronchitis patient,[1] and has since been reported as an emerging clinically relevant pathogen worldwide.The diagnosis of nocardial infections remains challenging due to nonspecific symptoms and low culture sensitivity,resulting in high mortality.[2] Herein,we report a case of N.cyriacigeorgica brain abscess in an immunosuppressed patient who was successfully treated with antibiotics and surgery. 展开更多
关键词 immunosuppressed patient antibiotics nocardial infections Nocardia cyriacigeorgica clinically relevant pathogen brain abscess chronic bronchitis metagenomic next generation sequencing
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Single-cell RNA sequencing of the post-spinal cord injury dorsal root ganglia in cynomolgus monkeys:Elucidation of the cellular immune microenvironment of the central nervous system 认领 引用
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作者 Yiming Ren Bo Li +6 位作者 Bo Yang Baoyou Fan Shenghui Huang Guidong Shi Liang Liu Zhijian Wei Shiqing Feng 《Neural Regeneration Research》 SCIE CAS CSCD 2026年第6期2506-2513,共8页
Few studies have investigated alterations in the immune cell microenvironment of the dorsal root ganglia following spinal cord injury and whether these modifications facilitate axonal regeneration.In this study,we use... Few studies have investigated alterations in the immune cell microenvironment of the dorsal root ganglia following spinal cord injury and whether these modifications facilitate axonal regeneration.In this study,we used a single-cell RNA sequencing dataset to create a comprehensive profile of the diverse cell types in the dorsal root ganglia and spinal cord of a mid-thoracic contusion injury model in cynomolgus monkeys.Cell communication analysis indicated that specific signaling events among various dorsal root ganglia cell types occur in response to spinal cord injury.Single-cell analysis using dimensionality reduction clustering identified distinct molecular signatures for nine cell types,including macrophage subpopulations,and differential gene expression profiles between dorsal root ganglia cells and spinal cord cells following spinal cord injury.The macrophage subpopulations were categorized into 11 clusters(MC0-MC10)based on differentially expressed genes,with the top 10 genes being ABCA6,RBMS3,EBF1,LAMA4,ANTXR2,LAMA2,SOX5,FOXP2,GHR,and APOD.MC0,MC1,and MC2 constituted the predominant macrophage populations.MC4,MC6,and MC9 were nearly absent in the spinal cord,but exhibited significant increases in the dorsal root ganglia post-spinal cord injury.Notably,these subpopulations possess a strong capacity for regulating axonal regeneration.The developmental progression of dorsal root ganglia macrophages after spinal cord injury was elucidated using cell trajectory and pseudo-time analyses.Genes such as EBF1(MC6 and MC9 marker),RBMS3(MC6 and MC9 marker),and ABCA6(MC6 marker)showed high expression levels in the critical pathways of macrophage function.Through ligand-receptor pair analysis,we determined that the effects of macrophages on microglia are predominantly mediated through interaction pairs(e.g.,SPP1-CD44,LAMC1-CD44,and FN1-CD44),potentially facilitating specific cellular communications within the immune microenvironment.The single-cell RNA sequencing dataset used in this study represents the first comprehensive transcriptional analysis of the dorsal root ganglia after spinal cord injury in cynomolgus monkeys,encompassing nearly all cell types within the dorsal root ganglia region.Using this dataset,we evaluated diverse subtypes of macrophages in the post-spinal cord injury dorsal root ganglia area and examined the signaling pathways that facilitate interactions among immune response-related macrophages in the dorsal root ganglia.Findings from this study provide a theoretical basis for understanding how the immune microenvironment influences the regenerative capacity of dorsal root ganglia neurons after spinal cord injury and offer novel insights into the complex processes underlying the pathobiology of spinal cord injury. 展开更多
关键词 cellular communication cellular microenvironment differentially expressed genes dorsal root ganglia immune cells macrophage microglia neurons single-cell sequence spinal cord injury
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