Neonicotinoid insecticides(NEOs)are a group of widely used insecticides in agriculture,livestock,and pest control for pets.However,increasing reports on their adverse effects on non-target organisms have aroused growi...Neonicotinoid insecticides(NEOs)are a group of widely used insecticides in agriculture,livestock,and pest control for pets.However,increasing reports on their adverse effects on non-target organisms have aroused growing public concerns.Therefore,it is necessary to evaluate NEO exposure and the related human health.This review summarized the human biomonitoring studies on NEOs,and the epidemiological,in vitro,and in vivo toxicological investigations on the health effects of NEOs.Human biomonitoring studies revealed significant changes in NEO detection frequencies,concentrations,and profiles across different human populations,suggesting potential variances in exposure patterns or metabolic processes.Certain metabolites may be more sensitive biomarkers than their parent molecules,as evidenced by higher levels of some NEO metabolites than those of their parent compounds.Although human epidemiological data has revealed associations between NEO exposure and diverse detrimental health outcomes including reproductive and developmental abnormalities,oxidative damage,etc.,the cross-sectional design of most studies cannot elucidate causality,necessitating further prospective research.In vitro and in vivo studies have shown various toxic effects of NEOs,including neurotoxicity,hepatotoxicity,reproductive toxicity,genotoxicity,etc.Further research on the molecular mechanisms beyond oxidative stress is worthwhile.Given metabolic activation,additional metabolites of both conventional and new generation NEOs as well as NEO-like compounds need to be determined,and their toxicities should be evaluated.This review can be contributed to the comprehensive understanding of the threat from extensive exposure to NEOs and proposed future research directions can aid to improve studies on health effects of NEOs.展开更多
Broflanilide is the first listed meta-diamide insecticide,which has shown potential in the field of pesticide research and development due to its unique structure and mechanism of action.The key to the development of ...Broflanilide is the first listed meta-diamide insecticide,which has shown potential in the field of pesticide research and development due to its unique structure and mechanism of action.The key to the development of the meta-diamide insecticides is the derivatization of various sites in the meta-diamide compounds.It is of great research significance to develop highly effective,broad spectrum,safe,and environmentally compatible meta-diamide insecticide using Broflanilide as a lead compound to obtain structurally diverse meta-diamide compounds.An overview of meta-diamide derivatives from the perspectives of insecticidal activities and mechanism of action is offered.Their potentials as a dominant active structure for development of insecticides are also discussed.展开更多
Exposure to different neonicotinoid insecticides(NNIs)can cause varying degrees of harm to mammals and may even be carcinogenic.Due to their similar molecular structures,it is not only difficult to distinguish NNIs in...Exposure to different neonicotinoid insecticides(NNIs)can cause varying degrees of harm to mammals and may even be carcinogenic.Due to their similar molecular structures,it is not only difficult to distinguish NNIs in analysis,but also cross-reactions can also occur.These cross-reactions cause the calibration curves to exhibit strong nonlinearities that cannot be fitted by usual mathematical models.Here,we present an electrochemical sensor array comprising three sensing units for the simultaneous determination of imidacloprid,thiamethoxam,and nitenpyram.The method eliminates cross-reaction with the aid of machine learning.The machine learning model comprises three components:the Douglas-Peucker algorithm for data compression,principal component analysis for classification,and an artificial neural network for quantification.The randomly assigned validation set showed a classification accuracy of 96.3%for the model.The prediction accuracy was 98.77%.The limit of detection was<0.037μmol/L,with a detection range from 0.1μmol/L to 200μmol/L.Finally,the spiked tea samples were tested,and a satisfactory agreement was obtained between the expected and predicted values.展开更多
Microbial pesticides are being increasingly adopted in modern agriculture as sustainable alternatives to synthetic chemical pesticides,featuring outstanding target specificity and low environmental risk.Their applicat...Microbial pesticides are being increasingly adopted in modern agriculture as sustainable alternatives to synthetic chemical pesticides,featuring outstanding target specificity and low environmental risk.Their application plays a vital role in rice production,enabling effective suppression of key rice insect pests including stem borers,leaf folders,and planthoppers,while preserving ecological balance.The research,development,and popularization of microbial insecticides facilitate green agricultural development,with numerous commercial formulations successfully launched worldwide.This review summarizes the research progress of microbial insecticides categorized as bacterial,fungal,viral,and metabolite-based agents,focusing on their practical application in rice insect pest control.Furthermore,it discusses prominent technical and practical bottlenecks restricting their large-scale promotion and prospects future development directions consistent with sustainable plant protection strategies.展开更多
Neonicotinoid insecticides(NEOs)have become an integral part of the global insecticide market due to their high efficiency and low toxicity.However,their environmental persistence has raised significant ecological con...Neonicotinoid insecticides(NEOs)have become an integral part of the global insecticide market due to their high efficiency and low toxicity.However,their environmental persistence has raised significant ecological concerns.Dongting Lake represents a vital freshwater lake in China,and its ecosystem health directly affects regional ecological balance and people’s livelihoods.This study systematically investigated the occurrence characteristics and ecological risks of NEOs in water bodies and sediments across the Dongting Lake basin.Based on surface water and sediment samples collected from 26 representative sampling sites,this study quantified nine NEOs using liquid chromatography triple quadrupole mass spectrometry.Furthermore,it assessed ecological risks posed by the NEOs using the risk quotient(RQ)method and fugacity modeling.The results revealed the presence of six NEOs in the water bodies:imidacloprid(IMI),acetamiprid(ACE),clothianidin(CLO),thiamethoxam(THIA),flonicamid(FLO),and dinotefuran(DIN).The total concentrations of these six NEOs averaged 275.11 ng/L.Five predominant NEOs(i.e.,IMI,THIA,ACE,CLO,and DIN)were identified in the sediments,with a mean concentration of 0.31 ng/g.The NEO concentrations in the water bodies across the Dongting Lake basin increased in the order of the Xiangjiang,Zishui,Yuanjiang,and Lishui rivers(collectively referred to as the Four Rivers),the mainstream of Dongting Lake,the Xinqiang River,the Miluo River,and the Hudu,Ouchi,and Songzi rivers(collectively referred to as the Three Outlets).Sediments from tributaries progressively accumulate in the lake.The ecological risk assessment identified IMI and DIN as the highest-risk compounds(RQ>1),with high-risk areas concentrated in the mainstream of Dongting Lake and the Ouchi,Miluo,and Hudu rivers.The fugacity model showed that IMI,ACE,and THIA are prone to diffuse from sediments to water bodies in most areas,with fugacity fractions(ff)values of greater than 0.5.In contrast,the mainstream of Dongting Lake acts as a sink of CLO and DIN(ff values:<0.5).Sediments at the lake’s outlet emerge as an important sink of NEOs.Based on the results of this study,it is advisable to strengthen the supervision of NEO applications in agricultural areas and to implement zonal control strategies.These measures will help reduce ecological risks and protect the safety of water ecosystems in the Dongting Lake region.展开更多
[Objective] The aim was to determine control effects of the 6 insecticides and recommended the optimal doses. [Method] In 2014, the control effects of sophocarpidine, pymetrozine, beta-cypermethrin, acetamiprid, imida...[Objective] The aim was to determine control effects of the 6 insecticides and recommended the optimal doses. [Method] In 2014, the control effects of sophocarpidine, pymetrozine, beta-cypermethrin, acetamiprid, imidacloprid, and chlor- pyrifos on wheat aphids were tested, and every pesticide was designed with three doses in Shou County, Anhui Province, to explore the significance of differences on control effects. [Result] The results showed that the control effects of the 6 insecti- cides were satisfied, and the insecticides were safe on wheat. [Conclusion] During initial diseasing stage of aphid, it is recommended to use sophocarpidine soluble concentrate (1.5%), pymetrozine water dispersible granule (50%), imidacloprid wet- table powder (25%), and acetamiprid wettable powder (5%), and chlorpyrifos missi- ble oil (40%), cypermethrin missible oil (4.5%) and imidacloprid wettable powder (25%) can be applied in peak-diseasing stage.展开更多
Objective:To determine the susceptibility and irritability level of malaria vector Anopheles sacharovi(An.sachrovi) to different insecticides in a malaria-prone area.Methods:Susceptibility and irritability levels of f...Objective:To determine the susceptibility and irritability level of malaria vector Anopheles sacharovi(An.sachrovi) to different insecticides in a malaria-prone area.Methods:Susceptibility and irritability levels of field collected strain of An.sacharovi to WHO standard papers of DDT 4%,dieldrin 0.4%,malathion 5%,fenitrothion 1%,permethrin 0.75%,and deltamethrin 0.05% were determined in East Azerbaijan of Iran during reemerging of malaria as described by WHO.Results:Results showed that at the diagnostic dose of insecticides this species exhibited resistance to DDT,tolerant to dieldrin and but somehow susceptible to fenitrothion,malathion, permethrin and deltamethrin.The results of irritability of this species to DDT,lambdacyhalothrin. permethrin cyfluthrin and deltamethrin revealed that DDT had had the most and deltamethrin the least irritancy effect.The average number of take offs/fly/minules for DDT was 0.8±0.2.The order of irritability for permethrin,lambdacyhalothrin,cyfluthrin and deltamethrin were 0.7±0.2,0.5±0.2, 0.5±0.3,and 0.2±0.1,respectively.Conclusions:Results of this study reveals the responsiveness of the main malaria vector to different insecticides.This phenomenon is depending on several factors such as type and background of insecticide used previously,insecticide properties,and physiology of the species.Careful monitoring of insecticide resistance and irritability level of species could provide a clue for appropriate selection of insecticide for malaria control.展开更多
To adapt to the rapid development of grass industry, improve the yield and quality of alfalfa and increase the benefits of alfalfa planting, the experiments on foliar fertilizers and plant growth regulators were condu...To adapt to the rapid development of grass industry, improve the yield and quality of alfalfa and increase the benefits of alfalfa planting, the experiments on foliar fertilizers and plant growth regulators were conducted, 2 suitable foliar fertilizers were screened out from 5. The results showed that the yield of alfalfa increased more than 20% under the treatments of KH2PO4 and Ausnutria, and the crude protein content increased significantly by 9.37%-10.62%, and crude fiber content was reduced by 5.91%-4.36%. As a result, KH2PO4 and Ausnutria were recommended as foliar fertilizers during the growth period of alfalfa with the concentration of 1 000 times and 500 times.展开更多
Background The cryptic nature of pink bollworm Pectinophora gossypiella(Saunders)larvae enables its reduced vul-nerability to insecticidal control.Further,the development of resistance against Bacillus thuringiensis(B...Background The cryptic nature of pink bollworm Pectinophora gossypiella(Saunders)larvae enables its reduced vul-nerability to insecticidal control.Further,the development of resistance against Bacillus thuringiensis(Bt)toxins posed a serious threat to transgenic cotton cultivation.This necessitated determining the critical timing of spray applications on the control effectiveness.This study assessed the influence of egg age(freshly laid vs.three-day-old)and the loca-tion of larvae(directly exposed to the insecticide residues on the boll rind vs.burrowed inside the bolls)on insecticide control efficacy.Results The results revealed a significant decrease in the ovicidal activity of tested insecticides with an increase in the age of eggs from one day old to three days old(paired t-test,P80%mortality)than the larvae exposed after they had bur-rowed inside the bolls(<49%mortality).The inhibitory effects of tested insecticides on developmental biology were more pronounced in the experiment on pre-larval release insecticide treatment compared with insecticide treatment given post-larval release and entry inside the bolls.Conclusion Egg age influences the insecticide susceptibility,as does the larval location,directly exposed vs bur-rowed inside the bolls.Older eggs and the larvae that had burrowed inside the green bolls of cotton were relatively less susceptible to the insecticide treatments.The toxic effects of insecticides on egg and larval stages were primar-ily ephemeral.These findings are significant for devising a comprehensive strategy for pink bollworm management on a sustainable basis.展开更多
An effective prediction pharmacophore model (RMS = 0.634, Correl = 0.893, Weight = 1.463, Config = 11.940) was successfully obtained by 3D-QSAR based on a series of nAChR (nicotinic acetylcholine receptors) agonis...An effective prediction pharmacophore model (RMS = 0.634, Correl = 0.893, Weight = 1.463, Config = 11.940) was successfully obtained by 3D-QSAR based on a series of nAChR (nicotinic acetylcholine receptors) agonists, which consists of a hydrogen- bonding acceptor, a hydrogen-bond donor, a hydrophobic aliphatic and a hydrophobic aromatic centre. This pharmacophore model may provide theoretical basis for designation and development of higher active insecticides.展开更多
Thin-film microextraction(TFME),a new geometry for solid-phase microextraction,has become an attractive sample-preparation technique.Compared to other microextraction approaches,the sensitivity of this technique was...Thin-film microextraction(TFME),a new geometry for solid-phase microextraction,has become an attractive sample-preparation technique.Compared to other microextraction approaches,the sensitivity of this technique was enhanced without sacrificing the sampling time due to the high surface area-tovolume ratio together with the increase of extraction-phase volume.In this paper,a new TFME method based on poly(vinylidene fluoride) membrane was developed for the extraction of benzoylurea insecticides(diflubenzuron,triflumuron,hexaflumuron and teflubenzuron) from water samples followed by their determination with high performance liquid chromatography-diode array detection.Under the optimal conditions,good linearity was observed over the concentration range of 0.5-100.0 ng/mL with correlation coefficient greater than 0.9994.The limits of detection(S/N = 3) of the method for the target analytes were 0.1 ng/mL.Mean recoveries ranged from 87.7% to 103.9% with relative standard deviations lower than 6.5%.The results indicated that the developed TFME method is simple,efficient,and cost effective.展开更多
The sweetpotato whitefly Bemisia tabaci(Hemiptera:Aleyrodidae)is an extremely polyphagous insect pest that causes significant crop losses in Israel and worldwide.B.tabaci is a species complex of which the B and Q b...The sweetpotato whitefly Bemisia tabaci(Hemiptera:Aleyrodidae)is an extremely polyphagous insect pest that causes significant crop losses in Israel and worldwide.B.tabaci is a species complex of which the B and Q biotypes are the most widespread and damaging worldwide.The change in biotype composition and resistance to insecticide in Israel was monitored during the years 2008-2010 to identify patterns in population dynamics that can be correlated with resistance outbreaks.The results show that B biotype populations dominate crops grown in open fields,while Q biotype populations gradually dominate crops grown in protected conditions such as greenhouses and nethouses,where resistance outbreaks usually develop after several insecticide applications.While in previous years,Q biotype populations were widely detected in many regions in Israel,significant domination of the B biotype across populations collected was observed during the year 2010,indicating the instability of the B.tabaci population from one year to another.Reasons for the changing dynamics and the shift in the relative abundance of B.tabaci biotype,and their resistance status,are discussed.展开更多
Objective: To evaluate the susceptibility of Anopheles stephensi(An. stephensi) Liston, the main malaria vector in southern Iran, to WHO recommended insecticides. Methods: Larvae of An. stephensi were collected from t...Objective: To evaluate the susceptibility of Anopheles stephensi(An. stephensi) Liston, the main malaria vector in southern Iran, to WHO recommended insecticides. Methods: Larvae of An. stephensi were collected from three different larval habitats in both urban and rural area of Bandar Abbas city and one rural area in Rudan county southern Iran. WHO standard method was used for evaluation of adult and larval mosquito susceptibility. Bendiocarb, permethrin, lambda-cyhalothrin, deltamethrin as insecticide and temephos and chlorpyriphos as larvicide were used at the diagnostic dosages recommended by WHO. Results: Findings of this study showed all larval populations of An. stephensi were completely susceptible to temephos and candidate for resistance to chlorpyriphos. Adult mosquitoes in rural areas of Bandar Abbas city were resistant to pyrethroid and carbamate insecticides. Conclusion: Comparison of the results of this survey with previous studies indicates that the resistance to pyrethroids and carbamates in this malaria endemic region is increasing. Wide use of pesticides in agriculture is certainly effective in increasing resistance. The inter-sectoral coordination and collaboration in health and agriculture seem to be necessary to manage insecticide resistance in malaria vectors.展开更多
Pesticides'overuse and misuse have been reported to induce ingredient variations in herbal medicine,which is now gaining attention in the medicinal field as a form of alternative medicine.To date,available studies...Pesticides'overuse and misuse have been reported to induce ingredient variations in herbal medicine,which is now gaining attention in the medicinal field as a form of alternative medicine.To date,available studies on pesticide-induced ingredient variations of herbal medicine are limited only on a few compounds and remain most others unexamined.In this study,a plant metabolomics-based strategy was performed to systematically explore the effects of two frequently used insecticides on the comprehensive constituents of Lonicerae Japonicae Flos(LJF),the flower buds of Lonicera japonica Thunb..Field trials were designed on a cultivating plot of L.japonica with controls and treatments of imidacloprid(IMI)and compound flonicamid and acetamiprid(CFA).Unbiased metabolite profiling was conducted by ultra-high performance liquid chromatography/quadrupole-Orbitrap mass spectrometer.After data pretreatment by automatic extraction and screening,a data matrix of metabolite features was submitted for statistical analyses.Consequently,29 metabolic markers,including chlorogenic acids,iridoids and organic acid-glucosides were obtained and characterized.The relative quantitative assay was subsequently performed to monitor their variations across flowering developments.This is the first study that systematically explored the insecticide-induced metabolite variations of LJF while taking into account the inherent variability of flowering development.The results were beneficial for holistic quality assessment of LJF and significant for guiding scientific use of pesticides in the large-scale cultivation.展开更多
Objective: To determine the susceptibility status of Anopheles maculipennis(An. maculipennis) against the major insecticides used in the health sectors in West Azarbaijan Province, Northwestern Iran.Methods: Unfed 3-5...Objective: To determine the susceptibility status of Anopheles maculipennis(An. maculipennis) against the major insecticides used in the health sectors in West Azarbaijan Province, Northwestern Iran.Methods: Unfed 3-5 days old adult females of An. maculipennis were collected across the West Azarbaijan Province and were subjected to evaluation of their susceptibility following World Health Organization recommended protocol against six insecticides(permethrin, deltamethrin, propoxur, bendiocarb, malathion and dieldrin) belonging to four different classes. Results: In this study, 916 specimens of An. maculipennis were examined against the insecticides which indicated that An. maculipennis was tolerant to permethrin, deltamethrin and dielderin, but displayed resistance against propoxur, bendiocarb and malathion. Conclusions: The pattern of resistance in An. maculipennis could be attributed to the agricultural landscapes, agricultural pesticides used and the exposure of the mosquitoes to insecticides. Logical cooperation is needed between the agriculture and health sectors to ensure the judicious use of pesticides in each sector and the management of probable resistance.展开更多
The combinative rate measurement of (3-[I125] iodotyrosyl) α-bungarotoxin was applied in the analysis of the relation between nerve acetylcholine receptor and three types of insecticide resistance in diamondback mo...The combinative rate measurement of (3-[I125] iodotyrosyl) α-bungarotoxin was applied in the analysis of the relation between nerve acetylcholine receptor and three types of insecticide resistance in diamondback moth, Plutella xylostella (L.). In the dimehypo-resistant strain and in the cartap-resistant strain, the nerve acetylcholine receptor showed the remarkable insensitivity to dimehypo and cartap, of which the binding rate to ligand was approximately 66 and 60%, respectively, of the susceptible strain. The sensitivity to deltamethrin in the deltamethrin-resistant strain did not show visible change. These results indicated that the decline in the sensitivity of nerve acetylcholine receptor to insecticide might be a potential mechanism to nereistoxin insecticides resistance in the diamondback moth.展开更多
Insecticides from different chemical groups were tested by laboratory bioassay to verify the percentage mortality of Helicoverpa armigera (Hubner 1808) (Lepidoptera: Noctuidae). The experiment was conducted in the Cro...Insecticides from different chemical groups were tested by laboratory bioassay to verify the percentage mortality of Helicoverpa armigera (Hubner 1808) (Lepidoptera: Noctuidae). The experiment was conducted in the Crop Science laboratory—Prof. CinobelinaElvas Campus—UFPI, Bom Jesus, PI, from January to June, 2013. The populations utilized came from the University’s own insect breeding laboratories. Third instar larvae of H. armigera were used to conduct the bioassay. The experimental design was fully randomized, with 13 treatments and four replications. Five larvae were used per replication, with 12 insecticides from 9 different chemical groups and a control. Each treatment consisted of three doses. The methods of application used were topical contact and ingestion in artificial diet. According to the results the percentage mortality of H. armigera larvae varied among the treatments. The results demonstrated that chlorpyrifos and spinosad were effective against third instar H. armigera larvae both on contact and by ingestion. Flubendiamide, acephate, methomyl, Bacillus thuringiensis, dimethoate, chlorantraniliprole and fipronil had good responses to control of H. armigera.展开更多
Currently, insecticides are considered as the primary approach for controlling western flower thrips, Frankliniella occidentalis(Pergande)(Thysanoptera: Thripidae). However, the heavy use of insecticides resulted...Currently, insecticides are considered as the primary approach for controlling western flower thrips, Frankliniella occidentalis(Pergande)(Thysanoptera: Thripidae). However, the heavy use of insecticides resulted in high insect resistance and serious environmental pollution. Given its characteristics of ease of operation and environmental friendliness, insect control using high temperature is receiving considerable renewed research interest. However, although the combination of insecticides and high temperature to control F. occidentalis has been studied before, few studies have focused on the short-term effect of such treatment. In a laboratory study, F. occidentalis adults and second-instar nymphs were exposed to 45℃ for 2 h. Then, their susceptibility to acetamiprid, spinosad, methomyl, and beta-cypermethrin was tested after different periods of recovery time(2–36 h). Additionally, the specific activity of three detoxification enzymes(esterase, glutathione S-transferase, and cytochrome p450(CYP) monooxygenase) of the treated insects was determined. The results indicated that the fluctuation of susceptibility to insecticides and detoxification enzyme activity during F. occidentalis recovery from heat shock are related. Furthermore, several recovery time points(2, 30, and 36 h) of significant susceptibility to four tested insecticides compared with the control were found during the treatment of adults that were heat-shocked. Recovery time points of higher susceptibility compared with the control depended on different insecticides during the second-instar nymph recovery from heat shock. Interestingly, the fluctuation of CYP monooxygenase activity exhibited a trend that was similar to the fluctuation of susceptibility to insecticides(especially spinosad) during the recovery from heat shock of adults. In addition, the glutathione S-transferase and CYP monooxygenase activity trend was similar to the trend of susceptibility to spinosad during the recovery from heat shock of second-instar nymphs. Our results provide a new approach for controlling F. occidentalis using the combined heat shock and insecticide. This effectively enhances the control efficiency of heat shock and significantly reduces the application of insecticides.展开更多
[Objective] The aim was to select insecticides effective in contrlling Pseudaulacaspis pentagona Infecting Pitaya. [Method] Efficacy effects of 11 insecticides in 22 concentrations were performed on Pseudaulacaspis pe...[Objective] The aim was to select insecticides effective in contrlling Pseudaulacaspis pentagona Infecting Pitaya. [Method] Efficacy effects of 11 insecticides in 22 concentrations were performed on Pseudaulacaspis pentagona Infecting Pitaya. [Result] When the insecticides were sprayed after 14 d, the treatment group with 22.4% spirotetramat SC at two concentrations proved the best, with control effects at 95.24% and 92.05%, followed by Nurelle at two concentrations, with control effects at 90.86% and 89.19%, and the control effects of chlorpyrifos (x2 000) was the poorest at only 67.08%. Therefore, it is feasible to make use of 22.4% spirotetramat SC, chlorpyrifos EC and Nurelle EC alternatively in controlling Pseudaulacaspis pentagona in case of pesticide resistance. [Conclusion] The research provided references for scientific use of insecticides in controlling Pseudaulacaspis pentagona Infecting Pitaya.展开更多
The resistance of field populations of Plutella xylostella, from the three vegetable producing areas (Nianyuxu Town of Yueyang City, Canggang Town of Changde City and Shatou Town of Yiyang City) in northern Hunan, t...The resistance of field populations of Plutella xylostella, from the three vegetable producing areas (Nianyuxu Town of Yueyang City, Canggang Town of Changde City and Shatou Town of Yiyang City) in northern Hunan, to seven insecticides was determined using leaf dipping method in door. The results showed that Plutella xylostella showed an extremely high-level resistance to beta-cypermethrin (resistance ratio, RR=257.13), a high-level resistance to abamectin (RR=135.15) and indoxacarb (RR=103.08) and a moderate-level resistance to chlorfenapyr and emamectin benzoate. But Plutella xylostella is relatively sensitive to chlorantraniliprole and Bacillus thuringiensis (Bt). Therefore, the prevention of Plutella xylostella in northern Hunan should focus on the alternative use of chlorfenapyr, emamectin benzoate, chlorantraniliprole and Bacillus thuringiensis and avoid the use of beta-cypermethrin so as to delay the generation and development of resistance to insecticides in Plutella xylostella.展开更多
基金supported by the National Natural Science Foundation of China(Nos.42207494 and 22193051)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB0750300)China Postdoctoral Science Foundation(No.2021M703402).
摘要Neonicotinoid insecticides(NEOs)are a group of widely used insecticides in agriculture,livestock,and pest control for pets.However,increasing reports on their adverse effects on non-target organisms have aroused growing public concerns.Therefore,it is necessary to evaluate NEO exposure and the related human health.This review summarized the human biomonitoring studies on NEOs,and the epidemiological,in vitro,and in vivo toxicological investigations on the health effects of NEOs.Human biomonitoring studies revealed significant changes in NEO detection frequencies,concentrations,and profiles across different human populations,suggesting potential variances in exposure patterns or metabolic processes.Certain metabolites may be more sensitive biomarkers than their parent molecules,as evidenced by higher levels of some NEO metabolites than those of their parent compounds.Although human epidemiological data has revealed associations between NEO exposure and diverse detrimental health outcomes including reproductive and developmental abnormalities,oxidative damage,etc.,the cross-sectional design of most studies cannot elucidate causality,necessitating further prospective research.In vitro and in vivo studies have shown various toxic effects of NEOs,including neurotoxicity,hepatotoxicity,reproductive toxicity,genotoxicity,etc.Further research on the molecular mechanisms beyond oxidative stress is worthwhile.Given metabolic activation,additional metabolites of both conventional and new generation NEOs as well as NEO-like compounds need to be determined,and their toxicities should be evaluated.This review can be contributed to the comprehensive understanding of the threat from extensive exposure to NEOs and proposed future research directions can aid to improve studies on health effects of NEOs.
基金supported by the National Natural Science Foundation of China(No.22271166)the Frontiers Science Center for New Organic Matter,Nankai University(No.63181206)for generous financial support for our programs。
摘要Broflanilide is the first listed meta-diamide insecticide,which has shown potential in the field of pesticide research and development due to its unique structure and mechanism of action.The key to the development of the meta-diamide insecticides is the derivatization of various sites in the meta-diamide compounds.It is of great research significance to develop highly effective,broad spectrum,safe,and environmentally compatible meta-diamide insecticide using Broflanilide as a lead compound to obtain structurally diverse meta-diamide compounds.An overview of meta-diamide derivatives from the perspectives of insecticidal activities and mechanism of action is offered.Their potentials as a dominant active structure for development of insecticides are also discussed.
基金supported by the National Natural Science Foundation of China(No.22008092)。
摘要Exposure to different neonicotinoid insecticides(NNIs)can cause varying degrees of harm to mammals and may even be carcinogenic.Due to their similar molecular structures,it is not only difficult to distinguish NNIs in analysis,but also cross-reactions can also occur.These cross-reactions cause the calibration curves to exhibit strong nonlinearities that cannot be fitted by usual mathematical models.Here,we present an electrochemical sensor array comprising three sensing units for the simultaneous determination of imidacloprid,thiamethoxam,and nitenpyram.The method eliminates cross-reaction with the aid of machine learning.The machine learning model comprises three components:the Douglas-Peucker algorithm for data compression,principal component analysis for classification,and an artificial neural network for quantification.The randomly assigned validation set showed a classification accuracy of 96.3%for the model.The prediction accuracy was 98.77%.The limit of detection was<0.037μmol/L,with a detection range from 0.1μmol/L to 200μmol/L.Finally,the spiked tea samples were tested,and a satisfactory agreement was obtained between the expected and predicted values.
基金supported by the Pioneer and Leading Goose R&D Program of Zhejiang Province,China(Grant No.2025C01118)the Zhejiang Provincial Natural Science Foundation of China(Grant No.LY23C140001).
摘要Microbial pesticides are being increasingly adopted in modern agriculture as sustainable alternatives to synthetic chemical pesticides,featuring outstanding target specificity and low environmental risk.Their application plays a vital role in rice production,enabling effective suppression of key rice insect pests including stem borers,leaf folders,and planthoppers,while preserving ecological balance.The research,development,and popularization of microbial insecticides facilitate green agricultural development,with numerous commercial formulations successfully launched worldwide.This review summarizes the research progress of microbial insecticides categorized as bacterial,fungal,viral,and metabolite-based agents,focusing on their practical application in rice insect pest control.Furthermore,it discusses prominent technical and practical bottlenecks restricting their large-scale promotion and prospects future development directions consistent with sustainable plant protection strategies.
基金jointly supported by project of the China Geological Survey(DD20243375,DD20230478)the Key Research and Development Program of Hunan Province(2023SK2066)the Natural Science Foundation of Hunan Province(2024JJ7620).
摘要Neonicotinoid insecticides(NEOs)have become an integral part of the global insecticide market due to their high efficiency and low toxicity.However,their environmental persistence has raised significant ecological concerns.Dongting Lake represents a vital freshwater lake in China,and its ecosystem health directly affects regional ecological balance and people’s livelihoods.This study systematically investigated the occurrence characteristics and ecological risks of NEOs in water bodies and sediments across the Dongting Lake basin.Based on surface water and sediment samples collected from 26 representative sampling sites,this study quantified nine NEOs using liquid chromatography triple quadrupole mass spectrometry.Furthermore,it assessed ecological risks posed by the NEOs using the risk quotient(RQ)method and fugacity modeling.The results revealed the presence of six NEOs in the water bodies:imidacloprid(IMI),acetamiprid(ACE),clothianidin(CLO),thiamethoxam(THIA),flonicamid(FLO),and dinotefuran(DIN).The total concentrations of these six NEOs averaged 275.11 ng/L.Five predominant NEOs(i.e.,IMI,THIA,ACE,CLO,and DIN)were identified in the sediments,with a mean concentration of 0.31 ng/g.The NEO concentrations in the water bodies across the Dongting Lake basin increased in the order of the Xiangjiang,Zishui,Yuanjiang,and Lishui rivers(collectively referred to as the Four Rivers),the mainstream of Dongting Lake,the Xinqiang River,the Miluo River,and the Hudu,Ouchi,and Songzi rivers(collectively referred to as the Three Outlets).Sediments from tributaries progressively accumulate in the lake.The ecological risk assessment identified IMI and DIN as the highest-risk compounds(RQ>1),with high-risk areas concentrated in the mainstream of Dongting Lake and the Ouchi,Miluo,and Hudu rivers.The fugacity model showed that IMI,ACE,and THIA are prone to diffuse from sediments to water bodies in most areas,with fugacity fractions(ff)values of greater than 0.5.In contrast,the mainstream of Dongting Lake acts as a sink of CLO and DIN(ff values:<0.5).Sediments at the lake’s outlet emerge as an important sink of NEOs.Based on the results of this study,it is advisable to strengthen the supervision of NEO applications in agricultural areas and to implement zonal control strategies.These measures will help reduce ecological risks and protect the safety of water ecosystems in the Dongting Lake region.
基金Supported by Pesticide Innovation and Highly Efficient Implementation Technology of Special Foundation for Anhui Talents Development(13C1109)~~
摘要[Objective] The aim was to determine control effects of the 6 insecticides and recommended the optimal doses. [Method] In 2014, the control effects of sophocarpidine, pymetrozine, beta-cypermethrin, acetamiprid, imidacloprid, and chlor- pyrifos on wheat aphids were tested, and every pesticide was designed with three doses in Shou County, Anhui Province, to explore the significance of differences on control effects. [Result] The results showed that the control effects of the 6 insecti- cides were satisfied, and the insecticides were safe on wheat. [Conclusion] During initial diseasing stage of aphid, it is recommended to use sophocarpidine soluble concentrate (1.5%), pymetrozine water dispersible granule (50%), imidacloprid wet- table powder (25%), and acetamiprid wettable powder (5%), and chlorpyrifos missi- ble oil (40%), cypermethrin missible oil (4.5%) and imidacloprid wettable powder (25%) can be applied in peak-diseasing stage.
基金supported by Tehran University of Medical Sciences
摘要Objective:To determine the susceptibility and irritability level of malaria vector Anopheles sacharovi(An.sachrovi) to different insecticides in a malaria-prone area.Methods:Susceptibility and irritability levels of field collected strain of An.sacharovi to WHO standard papers of DDT 4%,dieldrin 0.4%,malathion 5%,fenitrothion 1%,permethrin 0.75%,and deltamethrin 0.05% were determined in East Azerbaijan of Iran during reemerging of malaria as described by WHO.Results:Results showed that at the diagnostic dose of insecticides this species exhibited resistance to DDT,tolerant to dieldrin and but somehow susceptible to fenitrothion,malathion, permethrin and deltamethrin.The results of irritability of this species to DDT,lambdacyhalothrin. permethrin cyfluthrin and deltamethrin revealed that DDT had had the most and deltamethrin the least irritancy effect.The average number of take offs/fly/minules for DDT was 0.8±0.2.The order of irritability for permethrin,lambdacyhalothrin,cyfluthrin and deltamethrin were 0.7±0.2,0.5±0.2, 0.5±0.3,and 0.2±0.1,respectively.Conclusions:Results of this study reveals the responsiveness of the main malaria vector to different insecticides.This phenomenon is depending on several factors such as type and background of insecticide used previously,insecticide properties,and physiology of the species.Careful monitoring of insecticide resistance and irritability level of species could provide a clue for appropriate selection of insecticide for malaria control.
摘要To adapt to the rapid development of grass industry, improve the yield and quality of alfalfa and increase the benefits of alfalfa planting, the experiments on foliar fertilizers and plant growth regulators were conducted, 2 suitable foliar fertilizers were screened out from 5. The results showed that the yield of alfalfa increased more than 20% under the treatments of KH2PO4 and Ausnutria, and the crude protein content increased significantly by 9.37%-10.62%, and crude fiber content was reduced by 5.91%-4.36%. As a result, KH2PO4 and Ausnutria were recommended as foliar fertilizers during the growth period of alfalfa with the concentration of 1 000 times and 500 times.
摘要Background The cryptic nature of pink bollworm Pectinophora gossypiella(Saunders)larvae enables its reduced vul-nerability to insecticidal control.Further,the development of resistance against Bacillus thuringiensis(Bt)toxins posed a serious threat to transgenic cotton cultivation.This necessitated determining the critical timing of spray applications on the control effectiveness.This study assessed the influence of egg age(freshly laid vs.three-day-old)and the loca-tion of larvae(directly exposed to the insecticide residues on the boll rind vs.burrowed inside the bolls)on insecticide control efficacy.Results The results revealed a significant decrease in the ovicidal activity of tested insecticides with an increase in the age of eggs from one day old to three days old(paired t-test,P80%mortality)than the larvae exposed after they had bur-rowed inside the bolls(<49%mortality).The inhibitory effects of tested insecticides on developmental biology were more pronounced in the experiment on pre-larval release insecticide treatment compared with insecticide treatment given post-larval release and entry inside the bolls.Conclusion Egg age influences the insecticide susceptibility,as does the larval location,directly exposed vs bur-rowed inside the bolls.Older eggs and the larvae that had burrowed inside the green bolls of cotton were relatively less susceptible to the insecticide treatments.The toxic effects of insecticides on egg and larval stages were primar-ily ephemeral.These findings are significant for devising a comprehensive strategy for pink bollworm management on a sustainable basis.
摘要An effective prediction pharmacophore model (RMS = 0.634, Correl = 0.893, Weight = 1.463, Config = 11.940) was successfully obtained by 3D-QSAR based on a series of nAChR (nicotinic acetylcholine receptors) agonists, which consists of a hydrogen- bonding acceptor, a hydrogen-bond donor, a hydrophobic aliphatic and a hydrophobic aromatic centre. This pharmacophore model may provide theoretical basis for designation and development of higher active insecticides.
基金Financial supports from the National Natural Science Foundation of China(No.31171698)the Innovation Research Program of Department of Education of Hebei for Hebei Provincial Universities (No.LJRC009)+1 种基金the Scientific and Technological Research Foundation of Department of Education of Hebei Province(No. ZD20131033)the Natural Science Foundation of Hebei(No. B2012204028)
摘要Thin-film microextraction(TFME),a new geometry for solid-phase microextraction,has become an attractive sample-preparation technique.Compared to other microextraction approaches,the sensitivity of this technique was enhanced without sacrificing the sampling time due to the high surface area-tovolume ratio together with the increase of extraction-phase volume.In this paper,a new TFME method based on poly(vinylidene fluoride) membrane was developed for the extraction of benzoylurea insecticides(diflubenzuron,triflumuron,hexaflumuron and teflubenzuron) from water samples followed by their determination with high performance liquid chromatography-diode array detection.Under the optimal conditions,good linearity was observed over the concentration range of 0.5-100.0 ng/mL with correlation coefficient greater than 0.9994.The limits of detection(S/N = 3) of the method for the target analytes were 0.1 ng/mL.Mean recoveries ranged from 87.7% to 103.9% with relative standard deviations lower than 6.5%.The results indicated that the developed TFME method is simple,efficient,and cost effective.
基金This is contribution No.503/11 from the Agricultural Research Organization,the Volcani Center,Bet Dagan,Israel
摘要The sweetpotato whitefly Bemisia tabaci(Hemiptera:Aleyrodidae)is an extremely polyphagous insect pest that causes significant crop losses in Israel and worldwide.B.tabaci is a species complex of which the B and Q biotypes are the most widespread and damaging worldwide.The change in biotype composition and resistance to insecticide in Israel was monitored during the years 2008-2010 to identify patterns in population dynamics that can be correlated with resistance outbreaks.The results show that B biotype populations dominate crops grown in open fields,while Q biotype populations gradually dominate crops grown in protected conditions such as greenhouses and nethouses,where resistance outbreaks usually develop after several insecticide applications.While in previous years,Q biotype populations were widely detected in many regions in Israel,significant domination of the B biotype across populations collected was observed during the year 2010,indicating the instability of the B.tabaci population from one year to another.Reasons for the changing dynamics and the shift in the relative abundance of B.tabaci biotype,and their resistance status,are discussed.
基金financially supported by deputy for research,Tehran University of Medical Sciences,Project No.36251
摘要Objective: To evaluate the susceptibility of Anopheles stephensi(An. stephensi) Liston, the main malaria vector in southern Iran, to WHO recommended insecticides. Methods: Larvae of An. stephensi were collected from three different larval habitats in both urban and rural area of Bandar Abbas city and one rural area in Rudan county southern Iran. WHO standard method was used for evaluation of adult and larval mosquito susceptibility. Bendiocarb, permethrin, lambda-cyhalothrin, deltamethrin as insecticide and temephos and chlorpyriphos as larvicide were used at the diagnostic dosages recommended by WHO. Results: Findings of this study showed all larval populations of An. stephensi were completely susceptible to temephos and candidate for resistance to chlorpyriphos. Adult mosquitoes in rural areas of Bandar Abbas city were resistant to pyrethroid and carbamate insecticides. Conclusion: Comparison of the results of this survey with previous studies indicates that the resistance to pyrethroids and carbamates in this malaria endemic region is increasing. Wide use of pesticides in agriculture is certainly effective in increasing resistance. The inter-sectoral coordination and collaboration in health and agriculture seem to be necessary to manage insecticide resistance in malaria vectors.
基金supported by the National Key R&D Program of China(No.2017YFC1700800)China Postdoctoral Science Foundation(No.2019M651551)Shanghai Science and Technology Commission(Nos.18DZ2292200 and 18DZ2200-900)。
摘要Pesticides'overuse and misuse have been reported to induce ingredient variations in herbal medicine,which is now gaining attention in the medicinal field as a form of alternative medicine.To date,available studies on pesticide-induced ingredient variations of herbal medicine are limited only on a few compounds and remain most others unexamined.In this study,a plant metabolomics-based strategy was performed to systematically explore the effects of two frequently used insecticides on the comprehensive constituents of Lonicerae Japonicae Flos(LJF),the flower buds of Lonicera japonica Thunb..Field trials were designed on a cultivating plot of L.japonica with controls and treatments of imidacloprid(IMI)and compound flonicamid and acetamiprid(CFA).Unbiased metabolite profiling was conducted by ultra-high performance liquid chromatography/quadrupole-Orbitrap mass spectrometer.After data pretreatment by automatic extraction and screening,a data matrix of metabolite features was submitted for statistical analyses.Consequently,29 metabolic markers,including chlorogenic acids,iridoids and organic acid-glucosides were obtained and characterized.The relative quantitative assay was subsequently performed to monitor their variations across flowering developments.This is the first study that systematically explored the insecticide-induced metabolite variations of LJF while taking into account the inherent variability of flowering development.The results were beneficial for holistic quality assessment of LJF and significant for guiding scientific use of pesticides in the large-scale cultivation.
基金Supported by Urmia University of Medical Sciences,Urmia,Iran(Project No.1239)
摘要Objective: To determine the susceptibility status of Anopheles maculipennis(An. maculipennis) against the major insecticides used in the health sectors in West Azarbaijan Province, Northwestern Iran.Methods: Unfed 3-5 days old adult females of An. maculipennis were collected across the West Azarbaijan Province and were subjected to evaluation of their susceptibility following World Health Organization recommended protocol against six insecticides(permethrin, deltamethrin, propoxur, bendiocarb, malathion and dieldrin) belonging to four different classes. Results: In this study, 916 specimens of An. maculipennis were examined against the insecticides which indicated that An. maculipennis was tolerant to permethrin, deltamethrin and dielderin, but displayed resistance against propoxur, bendiocarb and malathion. Conclusions: The pattern of resistance in An. maculipennis could be attributed to the agricultural landscapes, agricultural pesticides used and the exposure of the mosquitoes to insecticides. Logical cooperation is needed between the agriculture and health sectors to ensure the judicious use of pesticides in each sector and the management of probable resistance.
基金the National Natural Science Foundation of China (30160050).
摘要The combinative rate measurement of (3-[I125] iodotyrosyl) α-bungarotoxin was applied in the analysis of the relation between nerve acetylcholine receptor and three types of insecticide resistance in diamondback moth, Plutella xylostella (L.). In the dimehypo-resistant strain and in the cartap-resistant strain, the nerve acetylcholine receptor showed the remarkable insensitivity to dimehypo and cartap, of which the binding rate to ligand was approximately 66 and 60%, respectively, of the susceptible strain. The sensitivity to deltamethrin in the deltamethrin-resistant strain did not show visible change. These results indicated that the decline in the sensitivity of nerve acetylcholine receptor to insecticide might be a potential mechanism to nereistoxin insecticides resistance in the diamondback moth.
基金The Foundation for Research Support of the State of Piauí(FAPEPI),the Federal University of Piaui(UFPI)for the structure and for financial support.
摘要Insecticides from different chemical groups were tested by laboratory bioassay to verify the percentage mortality of Helicoverpa armigera (Hubner 1808) (Lepidoptera: Noctuidae). The experiment was conducted in the Crop Science laboratory—Prof. CinobelinaElvas Campus—UFPI, Bom Jesus, PI, from January to June, 2013. The populations utilized came from the University’s own insect breeding laboratories. Third instar larvae of H. armigera were used to conduct the bioassay. The experimental design was fully randomized, with 13 treatments and four replications. Five larvae were used per replication, with 12 insecticides from 9 different chemical groups and a control. Each treatment consisted of three doses. The methods of application used were topical contact and ingestion in artificial diet. According to the results the percentage mortality of H. armigera larvae varied among the treatments. The results demonstrated that chlorpyrifos and spinosad were effective against third instar H. armigera larvae both on contact and by ingestion. Flubendiamide, acephate, methomyl, Bacillus thuringiensis, dimethoate, chlorantraniliprole and fipronil had good responses to control of H. armigera.
基金funded by the National Natural Science Foundation of China (31372003)the Shandong Modern Agricultural Technology and Industry System, China (SDAIT-02-021-11)+2 种基金the Taishan Scholarship Construction Engineering Special Fund, Chinathe Startup Fund for Distinguished Scholars (631316)supported by the Qingdao Agricultural University,China
摘要Currently, insecticides are considered as the primary approach for controlling western flower thrips, Frankliniella occidentalis(Pergande)(Thysanoptera: Thripidae). However, the heavy use of insecticides resulted in high insect resistance and serious environmental pollution. Given its characteristics of ease of operation and environmental friendliness, insect control using high temperature is receiving considerable renewed research interest. However, although the combination of insecticides and high temperature to control F. occidentalis has been studied before, few studies have focused on the short-term effect of such treatment. In a laboratory study, F. occidentalis adults and second-instar nymphs were exposed to 45℃ for 2 h. Then, their susceptibility to acetamiprid, spinosad, methomyl, and beta-cypermethrin was tested after different periods of recovery time(2–36 h). Additionally, the specific activity of three detoxification enzymes(esterase, glutathione S-transferase, and cytochrome p450(CYP) monooxygenase) of the treated insects was determined. The results indicated that the fluctuation of susceptibility to insecticides and detoxification enzyme activity during F. occidentalis recovery from heat shock are related. Furthermore, several recovery time points(2, 30, and 36 h) of significant susceptibility to four tested insecticides compared with the control were found during the treatment of adults that were heat-shocked. Recovery time points of higher susceptibility compared with the control depended on different insecticides during the second-instar nymph recovery from heat shock. Interestingly, the fluctuation of CYP monooxygenase activity exhibited a trend that was similar to the fluctuation of susceptibility to insecticides(especially spinosad) during the recovery from heat shock of adults. In addition, the glutathione S-transferase and CYP monooxygenase activity trend was similar to the trend of susceptibility to spinosad during the recovery from heat shock of second-instar nymphs. Our results provide a new approach for controlling F. occidentalis using the combined heat shock and insecticide. This effectively enhances the control efficiency of heat shock and significantly reduces the application of insecticides.
摘要[Objective] The aim was to select insecticides effective in contrlling Pseudaulacaspis pentagona Infecting Pitaya. [Method] Efficacy effects of 11 insecticides in 22 concentrations were performed on Pseudaulacaspis pentagona Infecting Pitaya. [Result] When the insecticides were sprayed after 14 d, the treatment group with 22.4% spirotetramat SC at two concentrations proved the best, with control effects at 95.24% and 92.05%, followed by Nurelle at two concentrations, with control effects at 90.86% and 89.19%, and the control effects of chlorpyrifos (x2 000) was the poorest at only 67.08%. Therefore, it is feasible to make use of 22.4% spirotetramat SC, chlorpyrifos EC and Nurelle EC alternatively in controlling Pseudaulacaspis pentagona in case of pesticide resistance. [Conclusion] The research provided references for scientific use of insecticides in controlling Pseudaulacaspis pentagona Infecting Pitaya.
摘要The resistance of field populations of Plutella xylostella, from the three vegetable producing areas (Nianyuxu Town of Yueyang City, Canggang Town of Changde City and Shatou Town of Yiyang City) in northern Hunan, to seven insecticides was determined using leaf dipping method in door. The results showed that Plutella xylostella showed an extremely high-level resistance to beta-cypermethrin (resistance ratio, RR=257.13), a high-level resistance to abamectin (RR=135.15) and indoxacarb (RR=103.08) and a moderate-level resistance to chlorfenapyr and emamectin benzoate. But Plutella xylostella is relatively sensitive to chlorantraniliprole and Bacillus thuringiensis (Bt). Therefore, the prevention of Plutella xylostella in northern Hunan should focus on the alternative use of chlorfenapyr, emamectin benzoate, chlorantraniliprole and Bacillus thuringiensis and avoid the use of beta-cypermethrin so as to delay the generation and development of resistance to insecticides in Plutella xylostella.