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In vitro screening of nutrients regulating sheep intramuscular angiogenesis,adipogenesis,and lipid deposition using an organoid model 认领 引用
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作者 Yating Li Xiaoying Sun +12 位作者 Yue Lv Jiaxin Liu Xinyi Mao Jinyan Yu Yanrong Feng Long Cheng Yifan Xiang Yu Xin Zhongzuo Huang Yichen Luo Yan Zhang Junxing Zhao Bo Wang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第1期457-472,共16页
Background The deposition of intramuscular fat(IMF)in livestock can enhance the flavor and tenderness of meat products,significantly increasing consumer satisfaction.To achieve this industrial trait,this study investi... Background The deposition of intramuscular fat(IMF)in livestock can enhance the flavor and tenderness of meat products,significantly increasing consumer satisfaction.To achieve this industrial trait,this study investigated the regulatory effects of 20 dietary nutrients on sheep IMF deposition using a 3D organoid culture model.Results Key nutrients enhancing angiogenesis,adipocyte differentiation,and lipid accumulation were identified through assessments of capillary sprouts development,mRNA expression,and Oil Red O staining.Vitamins C(VC),E(VE),and K1(VK1),guanidinoacetic acid(GAA),leucine(Leu),lysine(Lys),methionine(Met),N-carbamylglutamate(NCG),tryptophan(Trp),α-linolenic acid(ALA),linoleic acid(LA),cis-9,trans-11 conjugated linoleic acid(c9,t11-CLA),acetic acid(HAc),and sodium acetate(NaAc)stimulated while vitamins B9(VB9),D(VD),K2(VK2),taurine(Tau),and sodium butyrate(NaBu)inhibited angiogenesis(P<0.05).Furthermore,VC,VE,VK1,VK2,GAA,Leu,NCG,Trp,ALA,LA,and HAc enhanced adipocyte differentiation,with VE,VK1,GAA,Leu,LA,and HAc additionally elevating lipid accumulation(P<0.05).Conclusions Various nutrients play distinct regulatory roles in angiogenesis,adipocyte differentiation,and lipid accumulation.These findings provide a roadmap for further optimizing the production of marbled meat through nutritional intervention in actual livestock breeding production. 展开更多
关键词 Angiogenesis Intramuscular fat Meat quality Nutrient Sheep
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Vitamin A-activated PPARγ signaling enhances intramuscular fat accumulation by overriding AMPK-mediated inhibition in late-fattening beef cattle 认领 引用
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作者 Xinyue Yang Chengxing Zhang +6 位作者 Jizhe Tan Jinge Zhang Junhao Cui Yating Fan Nan Wang Yongcheng Jin Dongqiao Peng 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第3期1532-1553,共22页
Background Intramuscular fat(IMF)deposition determines beef marbling quality,with current industry practices relying on vitamin A(VA)restriction throughout fattening to enhance marbling development.This study challeng... Background Intramuscular fat(IMF)deposition determines beef marbling quality,with current industry practices relying on vitamin A(VA)restriction throughout fattening to enhance marbling development.This study challenges the conventional approach by investigating late-fattening vitamin A supplementation effects on marbling formation in Woking black cattle.Results Initial in vitro experiments using bovine skeletal muscle cells(BSMCs)demonstrated that all-trans-retinoic acid(ATRA)treatment during late differentiation(0.1-1μmol/L)enhanced lipid accumulation with upregulated PPARγ and FABP4 expression.In vivo trials with late-fattening VA supplementation(3,000 IU/kg DM)significantly improved marbling grades,achieving 75% high-grade marbling(A3 or above)with enhanced nutritionally beneficial fatty acids including EPA and DHA levels.Large-scale analysis using 336 genetically homogeneous cattle revealed that superior marbling development correlated with serum VA depletion after VA supplementation,indicating active utilization rather than restriction.A4-grade cattle showed significantly lower serum VA levels than A1-grade cattle,with coordinated upregulation of lipogenic proteins(FASN,SCD,ACACA,PPARγ,FABP4).Transcriptomic analysis unexpectedly revealed significant AMPK pathway activation alongside enhanced marbling development,contradicting conventional understanding of AMPK as an adipogenesis inhibitor.Functional validation using AMPK modulators in BSMCs confirmed that while AMPK inhibition(Compound C)dramatically enhanced VA-induced adipogenesis,AMPK activation(AICAR)suppressed lipogenesis,demonstrating AMPK functions as a negative feedback regulator during VAmediated adipogenesis rather than preventing intramuscular fat accumulation.Conclusions Strategic late-fattening VA supplementation enhances marbling development through PPARγ-mediated transcriptional networks,with AMPK serving as a metabolic sensor and negative feedback regulator rather than an absolute inhibitor.This stage-specific intervention achieved superior marbling quality and improved fatty acid composition in Woking black cattle,suggesting potential for optimization of premium beef production.Validation across diverse genetic backgrounds and production systems will be essential for broader industry implementation. 展开更多
关键词 AMPK pathway Beef cattle Intramuscular fat accumulation PPARγsignaling Vitamin A
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Gut microbiota and metabolites in lipid metabolism and intramuscular fat deposition:mechanisms and implications for meat quality 认领 引用 被引量：1
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作者 Xiaofeng Song Chenglong Jin +2 位作者 Ruifan Wu Yongjie Wang Xiaofan Wang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第1期60-80,共21页
Intramuscular fat(IMF)content serves as the key determinants of meat quality.Emerging evidence indicates that gut microbiota and their metabolites significantly influence IMF deposition levels by modulating host lipid... Intramuscular fat(IMF)content serves as the key determinants of meat quality.Emerging evidence indicates that gut microbiota and their metabolites significantly influence IMF deposition levels by modulating host lipid metabolism through multiple pathways,positioning microbial regulation as a pivotal target for meat quality improvement.However,existing studies remain fragmented,predominantly focusing on isolated mechanisms or correlations without a systematic view of the regulatory network.This review consolidates the core mechanisms through which microbiota-derived metabolites including short-chain fatty acids,bile acids,branched-chain amino acids,trimethylamine N-oxide,tryptophan derivatives,succinate,polyamines etc.,regulate IMF deposition and proposes a targeted intervention framework,the“gut microbiota/metabolites-IMF axis”.By integrating these insights,we provide a theoretical foundation and define practical research pathways to assess the potential of microbial-based strategies for improving meat quality in swine production. 展开更多
关键词 Bile acids Branched-chain amino acids Gut microbiota Intramuscular fat deposition Short-chain fatty acids
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Research advances in intramuscular fat deposition and chicken meat quality:genetics and nutrition 认领 引用 被引量：3
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作者 Jianlou Song Zengpeng Lv Yuming Guo 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第5期1921-1965,共45页
Chicken meat quality directly influences consumer acceptability and is crucial for the economic success of the poultry industry.Genetics and nutrition are key determinants of the meat quality traits in broilers.This r... Chicken meat quality directly influences consumer acceptability and is crucial for the economic success of the poultry industry.Genetics and nutrition are key determinants of the meat quality traits in broilers.This review summarizes the research advances in this field,with a focus on the genetic and nutritional foundations that regulate intramuscular fat(IMF)deposition and meat quality in chickens over the past decade.The effects of embryonic nutrition,both maternal nutrition and in ovo feeding(IOF),on skeletal muscle development,the IMF content,and meat quality traits in broilers are also discussed.In genetics,single-cell RNA sequencing revealed that de novo lipogenesis predominantly occurs in myocytes,which is key to the formation of IMF in chicken muscle tissue.Fatty acid synthase(FASN)is the key enzyme involved in this process.This discovery has reshaped the traditional understanding of intramuscular lipid metabolism in poultry.Key genes,proteins,and pathways,such as FASN,FABP4,PPARG,C/EBPα,SLC27A1;LPL,APOA1,COL1A1;PPAR and ECM–receptor interactions signaling,have been identified to regulate IMF content and distribution by modulating fatty acid metabolism and adipogenesis.LncHLFF was innovatively found to promote ectopic IMF deposition in chickens via exosome-mediated mechanisms without affecting abdominal fat deposition.MiR-27b-3p and miR-128-3p were found to inhibit adipogenic differentiation by targeting PPARG,thereby affecting IMF formation.In nutrition,nutrigenomics research has shown that fructose enhances IMF deposition by activating ChREBP,providing new targets for nutritional interventions.Adjusting dietary components,including energy,protein,amino acids,fatty acids,and phytochemicals(e.g.,rutin),has been shown to significantly improve meat quality in broilers.Maternal nutrition(e.g.,intake of energy,amino acids,vitamins,and trace elements)and IOF(e.g.,N-carbamylglutamate)have also been confirmed to significantly impact offspring meat quality,opening new avenues for improving embryonic nutrition.Based on these significant advancements,this review proposes strategies that integrate genetic and nutritional approaches.These strategies aim to modulate the differentiation fate of paraxial mesenchymal stem cells toward myogenic or adipogenic lineages and the interaction between muscle and adipose tissues.These insights would help to improve meat quality while ensuring the growth performance of broiler chickens. 展开更多
关键词 Chicken meat quality Embryonic nutrition Genetic regulation Intramuscular fat Nutritional intervention
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Intramuscular fat deposition in pig:A key target for improving pork quality 认领 引用 被引量：5
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作者 Qi Han Xingguo Huang +3 位作者 Jun He Yiming Zeng Jie Yin Yulong Yin 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2025年第12期4461-4483,共23页
Intramuscular fat(IMF)is an important economic trait for pork quality,affecting meat flavour,juiciness,and tenderness.Hence,the improvement of IMF content is one of the hotspots of animal science to provide better mea... Intramuscular fat(IMF)is an important economic trait for pork quality,affecting meat flavour,juiciness,and tenderness.Hence,the improvement of IMF content is one of the hotspots of animal science to provide better meat product.Here,we found that most IMF-related genes are enriched in lipid metabolism processes,including fatty acid transport and uptake,fatty acid beta oxidation,lipid synthesis,lipid storage,and lipolysis.PPAR and AMPK signalling pathways are identified to be responsible for IMF deposition.Genetics and nongenetic factors(i.e.,diets,gut microbiota,age,sex and management)also positively or negatively regulate the IMF content in pigs.Taken together,this review deepens our understanding of how these factors affect pig IMF deposition and provides valuable information for moderately increasing IMF content. 展开更多
关键词 pig intramuscular fat fatty acid metabolism genetic factors nongenetic factors
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A novel protein encoded by porcine circANKRD17 activates the PPAR pathway to regulate intramuscular fat metabolism 认领 引用 被引量：3
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作者 Xiao He Fang Xie +6 位作者 Ying Nie Xuefeng Wang Junyi Luo Ting Chen Qianyun Xi Yongliang Zhang Jiajie Sun 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第3期1160-1175,共16页
Background Intramuscular fat is an important factor in evaluating pork quality and varies widely among different pig breeds.However,the regulatory mechanism of circular RNAs(circRNAs)in lipid metabolism remains largel... Background Intramuscular fat is an important factor in evaluating pork quality and varies widely among different pig breeds.However,the regulatory mechanism of circular RNAs(circRNAs)in lipid metabolism remains largely unexplored.Results We combined circRNA-seq and Ribo-seq data to screen a total of 18 circRNA candidates with coding potential,and circANKRD17 was found to be significantly elevated in the longissimus dorsi muscle of Lantang piglets,with a length of 1,844 nucleotides.Using single-cell sequencing,we identified 477 differentially expressed genes in IMF cells between Lantang and Landrace piglets,with enrichment in the PPAR signaling pathway.These genes included FABP4,FABP5,CPT1A,and UBC,consistent with the high levels of acylcarnitines observed in the longissimus dorsi muscles of the Lantang breed,as determined by lipidomic analysis.Further in vitro and in vivo experiments indicated that circANKRD17 can regulate lipid metabolism through various mechanisms involving the PPAR pathway,including promoting adipocyte differentiation,fatty acid transport and metabolism,triglyceride synthesis,and lipid droplet formation and maturation.In addition,we discovered that circANKRD17 has an open reading frame and can be translated into a novel 571-amino-acid protein that promotes lipid metabolism.Conclusions Our research provides new insights into the role of protein-coding circANKRD17,especially concerning the metabolic characteristics of pig breeds with higher intramuscular fat content. 展开更多
关键词 CircRNAs Intramuscular fat Meat quality PPAR pathway
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Integrating parental genomes to reduce reference bias and identify intramuscular fat genes in Qinchuan Black pigs 认领 引用
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作者 Guangquan Lv Peiyu Yang +7 位作者 Ao Guo Minghao Cao Dong Li Zhe Liu Mingyu Wang Jingchun Sun Rongrong Ding Taiyong Yu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第6期2626-2640,共15页
Background Traditional genomic analysis relies on a single reference genome,which struggles to effectively characterize the genetic diversity among populations.This is due to the substantial genetic differences betwee... Background Traditional genomic analysis relies on a single reference genome,which struggles to effectively characterize the genetic diversity among populations.This is due to the substantial genetic differences between the genome of the studied species and the reference genome,potentially introducing reference bias.Results In this study,we focused on Guanzhong Black pigs(GZB),Danish Large White pigs(DLW),and their hybrid offspring,Qinchuan Black pigs(QCB).We provided two high-quality parental genomes at the chromosomal level and constructed a parental genomic reference panel to detect SNPs(single nucleotide polymorphisms),INDELs(insertions and deletions),and SVs(structural variations).Compared with the single-reference method,the integrated parental genomic strategy identified 5.48%more SNPs and 67.84%more INDELs.The uniformity of variant distribution and genome functional annotation remained consistent before and after integration,while the ratio of nonreferenceon-reference genotypes was also improved.In population genetic structure analysis,principal component analysis(PCA)of the three variant types(SNPs,INDELs,and SVs)exhibited good clustering effects,and ADMIXTURE analysis demonstrated consistent stratification.Selection signal analysis based on the integrated parental genomic strategy successfully identified more differentiated windows and positively selected genes.By leveraging multiple variant types and employing two selection signal methods,we jointly identified several novel intramuscular fat candidate genes(MSMO1,SMC6,CCDC158,KIT,CCNC,etc.),which could not be identified by the single-reference method alone.Functional validation of the gene MSMO1 revealed its role in promoting intramuscular adipocyte proliferation and inhibiting adipogenic differentiation.Conclusions This study is the first to construct a parental genomic reference panel specifically for pig hybrid populations,which significantly reduces reference bias and exhibits superior performance in downstream analyses.This strategy offers new possibilities for genomic selection breeding of livestock and establishes a methodological foundation for precisely dissecting complex traits in hybrid populations. 展开更多
关键词 Genotyping Intramuscular fat Parental genome Pig
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Quantification of intermuscular and intramuscular adipose tissue using magnetic resonance imaging after neurodegenerative disorders 认领 引用 被引量：2
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作者 Madoka Ogawa Robert Lester +1 位作者 Hiroshi Akima Ashraf S.Gorgey 《Neural Regeneration Research》 SCIE CAS CSCD 2017年第12期2100-2105,共6页
Ectopic adiposity has gained considerable attention because of its tight association with metabolic and cardiovascular health in persons with spinal cord injury（SCI）. Ectopic adiposity is characterized by the storag... Ectopic adiposity has gained considerable attention because of its tight association with metabolic and cardiovascular health in persons with spinal cord injury（SCI）. Ectopic adiposity is characterized by the storage of adipose tissue in non-subcutaneous sites. Magnetic resonance imaging（MRI） has proven to be an effective tool in quantifying ectopic adiposity and provides the opportunity to measure different adipose depots including intermuscular adipose tissue（IMAT） and intramuscular adipose tissue（Intra MAT） or intramuscular fat（IMF）. It is highly important to distinguish and clearly define these compartments, because controversy still exists on how to accurately quantify these adipose depots. Investigators have relied on separating muscle from fat pixels based on their characteristic signal intensities. A common technique is plotting a threshold histogram that clearly separates between muscle and fat peaks. The cut-offs to separate between muscle and fat peaks are still not clearly defined and different cut-offs have been identified. This review will outline and compare the Midpoint and Otsu techniques, two methods used to determine the threshold between muscle and fat pixels on T1 weighted MRI. The process of water/fat segmentation using the Dixon method will also be outlined. We are hopeful that this review will trigger more research towards accurately quantifying ectopic adiposity due to its high relevance to cardiometabolic health after SCI. 展开更多
关键词 intermuscular adipose tissue intramuscular adipose tissue intramuscular fat ectopic adiposity magnetic resonance imaging
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Developmental Changes of the FAS and HSL mRNA Expression and Their Effects on the Content of Intramuscular Fat in Kazak and Xinjiang Sheep 认领 引用 被引量：46
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作者 Yong Qiao Zhiguo Huang +5 位作者 Qifa Li Zhenshan Liu Chengi Hao Guoqing Shi Rong Dai Zhuang Xie 《Journal of Genetics and Genomics》 CAS 北大核心 2007年第10期909-917,共9页
Twenty-four male Kazak sheep and 30 Xinjiang fine wool sheep at different ages were selected to investigate the development-dependent expression levels of fatty acid synthase(FAS)gene and hormone-sensitive lipase(HSL)... Twenty-four male Kazak sheep and 30 Xinjiang fine wool sheep at different ages were selected to investigate the development-dependent expression levels of fatty acid synthase(FAS)gene and hormone-sensitive lipase(HSL)gene in muscle and their effects on the contents of intramuscular fat(IMF).Longissimus dorsal muscle was sampled to measure IMF and total RNA was extracted to determine FAS and HSL mRNA expression levels by real-time PCR.The results showed that:l)The IMF content increased continuously with growth and showed significant differences(P〈0.05)between different age groups in male Kazak sheep,but in Xinjiang fine wool sheep there was no such difference observed.Furthermore,the IMF contents in Kazak were much higher(P〈0.01)than that of the other breed from day 30 to 90.2)FAS mRNA expression level was the highest(P〈0.05)on day 0 in Kazak sheep and then declined with growth,in the other breed the gene showed a d‘ecline-rise-decline-rise'expression manner as the animals grew.HSL mRNA expression level had a similar model in two breeds,in Kazak sheep it was the highest on day 0(P〈0.05)and in Xinjiang fine wool sheep on day 30(P〈0.01),then both decreased after this term.3)In male Kazak sheep,FAS and HSL mRNA expression level were both negatively related to IMF content(r=-0.485(P=0.02),r=-0.423(P=0.05)),and the ratio of FAS/HSL expression exhibited significantly negatively related IMF contents.In male Xinjiang sheep,there were no obvious relationship between FAS and HSL expression and IMF content(P〉0.05). 展开更多
关键词 sheep intramuscular fat fatty acid synthase hormone-sensitive lipase real time PCR
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Single-base editing in IGF2 improves meat production and intramuscular fat deposition in Liang Guang Small Spotted pigs 认领 引用 被引量：11
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作者 Tianqi Duo Xiaohong Liu +11 位作者 Delin Mo Yu Bian Shufang Cai Min Wang Ruiqiang Li Qi Zhu Xian Tong Ziyun Liang Weilun Jiang Shiyi Chen Yaosheng Chen Zuyong He 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第1期108-126,共19页
Background Chinese indigenous pigs are popular with consumers for their juiciness,flavour and meat quality,but they have lower meat production.Insulin-like growth factor 2(IGF2) is a maternally imprinted growth factor... Background Chinese indigenous pigs are popular with consumers for their juiciness,flavour and meat quality,but they have lower meat production.Insulin-like growth factor 2(IGF2) is a maternally imprinted growth factor that promotes skeletal muscle growth by regulating cell proliferation and differentiation.A single nucleotide polymorphism(SNP) within intron 3 of porcine IGF2 disrupts a binding site for the repressor,zinc finger BED-type containing 6(ZBED6),leading to up-regulation of IGF2 and causing major effects on muscle growth,heart size,and backfat thickness.This favorable mutation is common in Western commercial pig populations,but absent in most Chinese indigenous pig breeds.To improve meat production of Chinese indigenous pigs,we used cytosine base editor 3(CBE3)to introduce IGF2 intron3-C3071T mutation into porcine embryonic fibroblasts(PEFs) isolated from a male Liang Guang Small Spotted pig(LGSS),and single-cell clones harboring the desired mutation were selected for somatic cell nuclear transfer(SCNT) to generate the founder line of IGF2T/T pigs.Results We found the heterozygous progeny IGF2C/T pigs exhibited enhanced expression of IGF2,increased lean meat by 18%-36%,enlarged loin muscle area by 3%-17%,improved intramuscular fat(IMF) content by 18%-39%,marbling score by 0.75-1,meat color score by 0.53-1.25,and reduced backfat thickness by 5%-16%.The enhanced accumulation of intramuscular fat in IGF2C/T pigs was identified to be regulated by the PI3K-AKT/AMPK pathway,which activated SREBP1 to promote adipogenesis.Conclusions We demonstrated the introduction of IGF2-intron3-C3071T in Chinese LGSS can improve both meat production and quality,and first identified the regulation of IMF deposition by IGF2 through SREBP1 via the PI3KAKT/AMPK signaling pathways.Our study provides a further understanding of the biological functions of IGF2and an example for improving porcine economic traits through precise base editing. 展开更多
关键词 CBE3 IGF2 Intramuscular fat Meat production PI3K-AKT/AMPK ZBED6
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MiR-146a-5p targeting SMAD4 and TRAF6 inhibits adipogenensis through TGF-β and AKT/mTORC1 signal pathways in porcine intramuscular preadipocytes 认领 引用 被引量：17
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作者 Que Zhang Rui Cai +2 位作者 Guorong Tang Wanrong Zhang Weijun Pang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2021年第1期220-235,共16页
Background: Intramuscular fat(IMF) content is a vital parameter for assessing pork quality. Increasing evidence has shown that microRNAs(miRNAs) play an important role in regulating porcine IMF deposition. Here, a nov... Background: Intramuscular fat(IMF) content is a vital parameter for assessing pork quality. Increasing evidence has shown that microRNAs(miRNAs) play an important role in regulating porcine IMF deposition. Here, a novel miRNA implicated in porcine IMF adipogenesis was found, and its effect and regulatory mechanism were further explored with respect to intramuscular preadipocyte proliferation and differentiation.Results: By porcine adipose tissue miRNA sequencing analysis, we found that miR-146a-5p is a potential regulator of porcine IMF adipogenesis. Further studies showed that miR-146a-5p mimics inhibited porcine intramuscular preadipocyte proliferation and differentiation, while the miR-146a-5p inhibitor promoted cell proliferation and adipogenic differentiation. Mechanistically, miR-146a-5p suppressed cell proliferation by directly targeting SMAD family member 4(SMAD4) to attenuate TGF-β signaling. Moreover, miR-146a-5p inhibited the differentiation of intramuscular preadipocytes by targeting TNF receptor-associated factor 6(TRAF6) to weaken the AKT/mTORC1 signaling downstream of the TRAF6 pathway.Conclusions: MiR-146a-5p targets SMAD4 and TRAF6 to inhibit porcine intramuscular adipogenesis by attenuating TGF-β and AKT/mTORC1 signaling, respectively. These findings provide a novel miRNA biomarker for regulating intramuscular adipogenesis to promote pork quality. 展开更多
关键词 Adipogenesis AKT/mTORC1 signal pathway MiR-146a-5p Porcine intramuscular fat SMAD4 TGF-βsignal pathway TRAF6
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Follicle-stimulating hormone increases the intramuscular fat content and expression of lipid biosynthesis genes in chicken breast muscle 认领 引用 被引量：7
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作者 Xiao-yan CUI Ying-ying LI +4 位作者 Ran-ran LIU Gui-ping ZHAO Mai-qing ZHENG Qing-he LI Jie WEN 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS CSCD 2016年第4期303-310,共8页
Intramuscular fat （IMF） is a crucial factor in the quality of chicken meat. The genetic basis underlying it is complex. Follicle-stimulating hormone （FSH）, well-known as an effector in reproductive tissues, was re... Intramuscular fat （IMF） is a crucial factor in the quality of chicken meat. The genetic basis underlying it is complex. Follicle-stimulating hormone （FSH）, well-known as an effector in reproductive tissues, was recently discovered to stimulate abdominal fat accumulation in chicken. The effect of FSH on IMF accumulation and the underlying molecular regulatory mechanisms controlling both IMF and abdominal fat deposition in vivo are largely unknown. In this study, two groups of chickens were treated with chicken FSH or a placebo. The lipid content of breast muscle, abdominal fat volume, and serum concentrations of FSH were examined. Related genes implicated in breast muscle and abdominal fat accumulation were also investigated. Compared to the control group, the triglyceride （TG） content of breast muscle and the percentage of abdominal fat in FSH-treated chickens were significantly increased by 64.9% and 56.5% （P〈0.01）, respectively. The FSH content in the serum of FSH-treated chickens was 2.1 times than that of control chickens （P〈0.01）. Results from quantitative real-time polymerase chain reaction （qRT-PCR） assays showed that relative expression levels of fatty acid synthase （FAS）, lipoprotein lipase （LPL）, diacylglycerol acyltransferase 2 （DGAT2）, adipocyte fatty acid binding protein （A-FABP）, and peroxisome proliferator-activated receptor ~ （PPARy） were significantly upregulated in breast muscle following FSH treatment （P〈0.01）. Treatment with FSH also signifi- cantly increased relative expression levels of FAS, LPL, DGA T2, A-FABP, and PPARy in abdominal fat tissue （P〈0.05） The results of principal component analysis （PCA） for gene expression （breast muscle and abdominal fat） showed that the control and FSH treatment groups were well separated, which indicated the reliability of the data. This study demonstrates that FSH plays an important role in IMF accumulation in female chickens, which likely involves the regulation of biosynthesis genes related to lipid metabolism. 展开更多
关键词 Follicle-stimulating hormone Intramuscular fat Abdominal fat Chickens mRNA expression
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A selected population study reveals the biochemical mechanism of intramuscular fat deposition in chicken meat 认领 引用 被引量：8
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作者 Huanxian Cui Lu Liu +7 位作者 Xiaojing Liu Yongli Wang Na Luo Xiaodong Tan Yuting Zhu Ranran Liu Guiping Zhao Jie Wen 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2022年第5期1282-1292,共11页
Background:Increasing intramuscular fat(IMF)is an important strategy to improve meat quality,but the regulation mechanism of IMF deposition needs to be systematically clarified.Results:A total of 520 chickens from a s... Background:Increasing intramuscular fat(IMF)is an important strategy to improve meat quality,but the regulation mechanism of IMF deposition needs to be systematically clarified.Results:A total of 520 chickens from a selected line with improved IMF content and a control line were used to investigate the biochemical mechanism of IMF deposition in chickens.The results showed that the increased IMF would improve the flavor and tenderness quality of chicken meat.IMF content was mainly determined both by measuring triglyceride(TG)and phospholipid(PLIP)in muscle tissue,but only TG content was found to be decisive for IMF deposition.Furthermore,the increase in major fatty acid(FA)components in IMF is mainly derived from TGs(including C16:0,C16:1,C18:1n9c,and C18:2n6c,etc.),and the inhibition of certain very-long-chain FAs would help to IMF/TG deposition.Conclusions:Our study elucidated the underlying biochemical mechanism of IMF deposition in chicken:Prevalent accumulation of long-chain FAs and inhibitions of medium-chain FAs and very long chain FA would jointly result in the increase of TGs with the FA biosynthesis and cellular uptake ways.Our findings will guide the production of high-quality chicken meat. 展开更多
关键词 Biochemical mechanism Chicken Fatty acid composition Intramuscular fat
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Vitamin A regulates intramuscular adipose tissue and muscle development:promoting high-quality beef production 认领 引用 被引量：14
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作者 Dong Qiao Peng Stephen B.Smith Hong Gu Lee 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2021年第3期1138-1147,共10页
During growth in cattle,the development of intramuscular adipose tissue and muscle is dependent upon cell hyperplasia(increased number of adipocytes)and hypertrophy(increased size of adipocytes).Based on the results o... During growth in cattle,the development of intramuscular adipose tissue and muscle is dependent upon cell hyperplasia(increased number of adipocytes)and hypertrophy(increased size of adipocytes).Based on the results of previous studies,other adipose tissue depots(e.g.,perirenal and subcutaneous)develop from the fetal stage primarily as brown adipose tissue.The hyperplastic stage of intramuscular adipose is considered to develop from late pregnancy,but there is no evidence indicating that intramuscular adipose tissue develops initially as brown adipose tissue.Hyperplastic growth of intramuscular adipose continues well into postweaning and is dependent on the timing of the transition to grain-based diets;thereafter,the late-stage development of intramuscular adipose tissue is dominated by hypertrophy.For muscle development,hyperplasia of myoblasts lasts from early(following development of somites in the embryo)to middle pregnancy,after which growth of muscle is the result of hypertrophy of myofibers.Vitamin A is a fat-soluble compound that is required for the normal immunologic function,vision,cellular proliferation,and differentiation.Here we review the roles of vitamin A in intramuscular adipose tissue and muscle development in cattle.Vitamin A regulates both hyperplasia and hypertrophy in in vitro experiments.Vitamin A supplementation at the early stage and restriction at fattening stage generate opposite effects in the beef cattle.Appropriate vitamin A supplementation and restriction strategy increase intramuscular adipose tissue development(i.e.,marbling or intramuscular fat)in some in vivo trials.Besides,hyperplasia and hypertrophy of myoblasts/myotubes were affected by vitamin A treatment in in vitro trials.Additionally,some studies reported an interaction between the alcohol dehydrogenase-1C(ADH1C)genotype and vitamin A feed restriction for the development of marbling and/or intramuscular adipose tissue,which was dependent on the timing and level of vitamin A restriction.Therefore,the feed strategy of vitamin A has the visible impact on the marbling and muscle development in the cattle,which will be helpful to promote the quality of the beef. 展开更多
关键词 Adipose tissue Cattle Intramuscular adipose tissue Muscle development Vitamin A
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Dietary calcium supplementation promotes the accumulation of intramuscular fat 认领 引用 被引量：6
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作者 Zhiwang Zhang Tingli Pan +5 位作者 Yu Sun Siqi Liu Ziyi Song Haojie Zhang Yixing Li Lei Zhou 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2022年第2期536-550,共15页
Background:In the livestock industry,intramuscular fat content is a key factor affecting meat quality.Many studies have shown that dietary calcium supplementation is closely related to lipid metabolism.However,few stu... Background:In the livestock industry,intramuscular fat content is a key factor affecting meat quality.Many studies have shown that dietary calcium supplementation is closely related to lipid metabolism.However,few studies have examined the relationship between dietary calcium supplementation and intramuscular fat accumulation.Methods:Here,we used C2C12 cells,C57BL/6 mice(n=8)and three-way cross-breeding pigs(Duroc×Landrace×Large white)(n=10)to study the effect of calcium addition on intramuscular fat accumulation.In vitro,we used calcium chloride to adjust the calcium levels in the medium(2 mmol/L or 3 mmol/L).Then we measured various indicators.In vivo,calcium carbonate was used to regulate calcium levels in feeds(Mice:0.5%calcium or 1.2%calcium)(Pigs:0.9%calcium or 1.5%calcium).Then we tested the mice gastrocnemius muscle triglyceride content,pig longissimus dorsi muscle meat quality and lipidomics.Results:In vitro,calcium addition(3 mmol/L)had no significant effect on cell proliferation,but promoted the differentiation of C2C12 cells into slow-twitch fibers.Calcium supplementation increased triglyceride accumulation in C2C12 cells.Calcium addition increased the number of mitochondria and also increased the calcium level in the mitochondria and reduced the of key enzymes activity involved inβ-oxidation such as acyl-coenzyme A dehydrogenase.Decreasing mitochondrial calcium level can alleviate lipid accumulation induced by calcium addition.In addition,calcium addition also reduced the glycolytic capacity and glycolytic conversion rate of C2C12 cells.In vivo,dietary calcium supplementation(1.2%)promoted the accumulation of triglycerides in the gastrocnemius muscle of mice.Dietary calcium supplementation(1.5%)had no effect on pig weight,but significantly improved the flesh color of the longissimus dorsi muscle,reduced the backfat thickness and increased intramuscular fat content in pigs.Besides,calcium addition had no effect on longissimus dorsi pH,electrical conductivity and shear force.Conclusions:These results suggest that calcium addition promotes intramuscular fat accumulation by inhibiting the oxidation of fatty acids.These findings provide a new tool for increasing intramuscular fat content and an economical strategy for improving meat quality. 展开更多
关键词 C2C12 Calcium Intramuscular fat Meat quality Pig
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RNA-seq analysis reveals the critical role of the novel lnc RNA BIANCR in intramuscular adipogenesis through the ERK1/2 signaling pathway 认领 引用 被引量：5
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作者 Xinhao Ma Xinran Yang +6 位作者 Dianqi Zhang Wenzhen Zhang Xiaoyu Wang Kuncheng Xie Jie He Chugang Mei Linsen Zan 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2023年第3期917-935,共19页
Background Long non-coding RNAs(lncRNAs)regulate numerous biological processes,including adipogenesis.Research on adipogenesis will assist in the treatment of human metabolic diseases and improve meat quality in lives... Background Long non-coding RNAs(lncRNAs)regulate numerous biological processes,including adipogenesis.Research on adipogenesis will assist in the treatment of human metabolic diseases and improve meat quality in livestock,such as the content of intramuscular fat(IMF).However,the significance of lncRNAs in intramuscular adipogenesis remains unclear.This research aimed to reveal the lncRNAs transcriptomic profiles in the process of bovine intramuscular adipogenesis and to identify the lncRNAs involved in the adipogenesis of bovine intramuscular adipocytes.Results In this research,a landscape of lncRNAs was identified with RNA-seq in bovine intramuscular adipocytes at four adipogenesis stages(0 d,3 d,6 d,and 9 d after differentiation).A total of 7035 lncRNAs were detected,including 3396 novel lncRNAs.Based on the results of differential analysis,co-expression analysis,and functional prediction,we focused on the bovine intramuscular adipogenesis-associated long non-coding RNA(BIANCR),a novel lncRNA that may have an important regulatory function.The knockdown of BIANCR inhibited proliferation and promoted apoptosis of intramuscular preadipocytes.Moreover,BIANCR knockdown inhibited intramuscular adipogenesis by regulating the ERK1/2 signaling pathway.Conclusion This study obtained the landscape of lncRNAs during adipogenesis in bovine intramuscular adipocytes.BIANCR plays a crucial role in adipogenesis through the ERK1/2 signaling pathway.The results are noteworthy for improving beef meat quality,molecular breeding,and metabolic disease research. 展开更多
关键词 Adipogenesis BIANCR Bovine Intramuscular adipocyte LncRNAs
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Identification of porcine fast/slow myogenic exosomes and their regulatory effects on lipid accumulation in intramuscular adipocytes 认领 引用 被引量：5
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作者 Tiantian Zhao Tingting Tian +7 位作者 He Yu Chaoyue Cao Ziyi Zhang Zhaozhao He Zeqiang Ma Rui Cai Fengna Li Weijun Pang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第4期1512-1527,共16页
Background Pork quality is affected by the type of muscle fibers, which is closely related to meat color, tenderness and juiciness. Exosomes are tiny vesicles with a diameter of approximately 30–150 nm that are secre... Background Pork quality is affected by the type of muscle fibers, which is closely related to meat color, tenderness and juiciness. Exosomes are tiny vesicles with a diameter of approximately 30–150 nm that are secreted by cells and taken up by recipient cells to mediate communication. Exosome-mediated muscle-fat tissue crosstalk is a newly discovered mechanism that may have an important effect on intramuscular fat deposition and with that on meat quality. Various of adipose tissue-derived exosomes have been discovered and identified, but the identification and function of muscle exosomes, especially porcine fast/slow myotube exosomes, remain unclear. Here, we first isolated and identified exosomes secreted from porcine extensor digitorum longus(EDL) and soleus(SOL), which represent fast and slow muscle, respectively, and further explored their effects on lipid accumulation in longissimus dorsi adipocytes.Results Porcine SOL-derived exosomes(SOL-EXO) and EDL-derived exosomes(EDL-EXO) were first identified and their average particle sizes were approximately 84 nm with double-membrane disc-shapes as observed via transmission electron microscopy and scanning electron microscopy. Moreover, the intramuscular fat content of the SOL was greater than that of the EDL at 180 days of age, because SOL intramuscular adipocytes had a stronger lipid-accumulating capacity than those of the EDL. Raman spectral analysis revealed that SOL-EXO protein content was much greater than that of EDL-EXO. Proteomic sequencing identified 72 proteins that were significantly differentially expressed between SOL-EXO and EDL-EXO, 31 of which were downregulated and 41 of which were upregulated in SOL-EXO.Conclusions Our findings suggest that muscle-fat tissue interactions occur partly via SOL-EXO promoting adipogenic activity of intramuscular adipocytes. 展开更多
关键词 Adipogenesis Exosome Extensor digitorum longus Intramuscular adipocyte Muscle-fat tissue interaction Pig Soleus
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Developmental Changes of Col3a1 mRNA Expression in Muscle and Their Association with Intramuscular Collagen in Pigs 认领 引用 被引量：6
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作者 Xinjian Bao Yongqing Zeng +5 位作者 Shudong Wei Gang Wang Chanjuan Liu Yanxiao Sun Qimei Chen Hua Li 《Journal of Genetics and Genomics》 CAS 北大核心 2007年第3期223-228,共6页
Eighty-four castrated boars including Laiwu Black(LW)(weight 30-90 kg,n=6)and Lulai Black(LL)(weight 40-100 kg,n=6)were used to study the developmental changes of collagen typeⅢalpha 1(Col3al)mRNA expression in the m... Eighty-four castrated boars including Laiwu Black(LW)(weight 30-90 kg,n=6)and Lulai Black(LL)(weight 40-100 kg,n=6)were used to study the developmental changes of collagen typeⅢalpha 1(Col3al)mRNA expression in the muscle and their association with intramuscular collagen(IMC).The muscle total RNA was extracted to determine the abundance of Col3al mRNA using relative quantitative RT-PCR withβ-actin mRNA as the internal standard.The results indicated that the developmental patterns of muscle Col3al mRNA in LW and LL pigs were similar.The abundance of Col3al mRNA increased with body weight,but decreased a little at 70 kg and 80 kg phases for LW and LL,respectively.On the whole,the expression level of Col3al mRNA in muscle of LW was higher than that of LL(P〈0.05).Correlation analysis showed that the expression of Col3al mRNA in muscle was positively correlated with total and insoluble IMC,but was negatively correlated with IMC solubility for LW pigs(P〈0.01)and LL pigs(P〈0.05),respectively.These results suggest that the muscle Col3al gene expression is affected by body weight and genotype and has important effect on IMC content and characteristics. 展开更多
关键词 pig Laiwu Black intramuscular collagen Col3al gene development RT-PCR
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Changes of Intramuscular Fat Composition,Lipid Oxidation and Lipase Activity in Biceps femoris and Semimembranosus of Xuanwei Ham During Controlled Salting Stages 认领 引用 被引量：5
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作者 WANG Zhen-yu GAO Xiao-guang +2 位作者 ZHANG Ji-hong ZHANG De-quan MA Chang-wei 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2013年第11期1993-2001,共9页
Fatty acid composition of neutral lipids （NLs）, phospholipids （PLs） and free fatty acids （FFAs） from intramuscular fat （IMF）, lipid oxidation and lipase activity in muscle Semimembranosus （SM） and msucle Bic... Fatty acid composition of neutral lipids （NLs）, phospholipids （PLs） and free fatty acids （FFAs） from intramuscular fat （IMF）, lipid oxidation and lipase activity in muscle Semimembranosus （SM） and msucle Biceps femoris （BF） of dry-cured Xuanwei ham during the 90-d salting stages were analysed. The salt content increased from 0.34 to 3.52%in BF and from 0.10 to 5.42%in SM during the 90 d salting stage, respectively. PLs of IMF in both BF and SM decreased 54.70%（P〈0.001） and 34.64%（P〈0.05）, furthermore, the saturated fatty acids （SFA）, monounsaturated fatty acids （MUFA） and polyunsaturated fatty acids （PUFA） of PLs in both muscles were hydrolysed almost isochronously. FFAs were increased from 0.46 g 100 g-1 lipids to 2.92 g 100 g-1 lipids in BF at the end of salting, which was lower than SM （from 1.29 g 100 g-1 lipids to 9.70 g 100 g-1 lipids）. The activities of acid lipase, neutral lipase and acid phospholipase all remained active in the 90 d. The thiobarbituric acid reactive substances （TBARS） was slowly increased to 1.34 mg kg-1 muscle in BF and to 2.44 mg kg-1 muscle in SM during the salting stage. In conclusion, the controlled salting process prompted the hydrolysis of PLs of IMF notably and increased the lipid oxidation of muscles within some limits. 展开更多
关键词 Xuanwei ham intramuscular fat lipid oxidation fatty acid composition salting
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TIMP2 promotes intramuscular fat deposition by regulating the extracellular matrix in chicken 认领 引用 被引量：3
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作者 CUI Huan-xian LUO Na +4 位作者 GUO Li-ping LIU Lu XING Si-yuan ZHAO Gui-ping WEN Jie 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2023年第3期853-863,共11页
The interaction between myocytes and intramuscular adipocytes is a hot scientific topic. Using a co-culture system, this study aims to investigate the regulation of intramuscular fat deposition in chicken muscle tissu... The interaction between myocytes and intramuscular adipocytes is a hot scientific topic. Using a co-culture system, this study aims to investigate the regulation of intramuscular fat deposition in chicken muscle tissue through the interaction between myocyte and adipocyte and identify important intermediary regulatory factors. Our proteomics data showed that the protein expression of tissue inhibitor of metalloproteinases 2(TIMP2) increased significantly in the culture medium of the co-culture system, and the content of lipid droplets was more in the co-culture intramuscular adipocytes. In addition, TIMP2 was significantly upregulated(P<0.01) in muscle tissue of individuals with high intramuscular fat content.Weighted gene co-expression network analysis revealed that TIMP2 was mainly involved in the extracellular matrix receptor interaction signaling pathway and its expression was significantly correlated with triglyceride, intramuscular fat,C14:0, C14:1, C16:0, C16:1, and C18:1n9C levels. Additionally, TIMP2 was co-expressed with various representative genes related to lipid metabolism(such as ADIPOQ, SCD, ELOVL5, ELOVL7, and LPL), as well as certain genes involved in extracellular matrix receptor interaction(such as COL1A2, COL4A2, COL5A1, COL6A1, and COL6A3), which are also significantly upregulated(P<0.05 or P<0.01) in muscle tissue of individuals with high intramuscular fat content.Our findings reveal that TIMP2 promotes intramuscular fat deposition in muscle tissue through the extracellular matrix receptor interaction signaling pathway. 展开更多
关键词 TIMP2 ECM intramuscular adipocytes muscle satellite cells chicken
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