Some visitors to India often experience diarrhea and attribute it to Indian food;however,murine food allergy model have shown that diarrhea occurring after consumption of Moringa may be due to an allergic reaction.The...Some visitors to India often experience diarrhea and attribute it to Indian food;however,murine food allergy model have shown that diarrhea occurring after consumption of Moringa may be due to an allergic reaction.The findings of this study indicate that Moringa leaf protein(Mo-Pr)has the potential to sensitize BALB/c mice.Mo-Pr has been demonstrated to stimulate the mice's production of Mo-Pr-specific immunoglobulin E,promote mast cell degranulation,and induce the release of histamine and other inflammatory mediators.Mo-Pr has been demonstrated to promote the proliferation and differentiation of Th2 cells while simultaneously inhibiting the proliferation and differentiation of Thl and Treg cells.The sera Western blot shown that the significant band Mo-Pr molecule weight is 36 kDa respectively,which was identified as fructose-1,6-bisphosphate aldolase by liquid chromatographytandem mass spectrometry.展开更多
特藏资源已跻身图书馆的核心竞争力之列。晚清民国的“高龄”特藏面临严重损毁危机,传统研究缺乏从图书馆、档案馆、博物馆(Libraries,Archives and Museums,LAM)协同视角探索全流程活化路径。中国地质大学(武汉)通过机构改革,合并图书...特藏资源已跻身图书馆的核心竞争力之列。晚清民国的“高龄”特藏面临严重损毁危机,传统研究缺乏从图书馆、档案馆、博物馆(Libraries,Archives and Museums,LAM)协同视角探索全流程活化路径。中国地质大学(武汉)通过机构改革,合并图书馆、档案馆、校史馆、博物馆为图书档案与文博部,形成一部辖四馆的组织架构,通过治理协同、资源协同和业务协同对具有地学特色的“高龄”特藏文献进行濒危抢救和价值创造,取得良好成效。中国地质大学(武汉)的实践表明:优化行政治理、打造文化场馆集群,强化场馆资源聚类和业务协同,不失为高校文化机构开展特藏建设的可行路径之一。展开更多
People living long-term in areas with UV will cause premature photoaging.An abnormal reduction in autophagy is a key feature of photoaging,and p38 MAPK has been regarded as a key regulator of autophagy.Isothiocyanate ...People living long-term in areas with UV will cause premature photoaging.An abnormal reduction in autophagy is a key feature of photoaging,and p38 MAPK has been regarded as a key regulator of autophagy.Isothiocyanate is one of the main active components of Moringa oleifera Lam.seeds.Studies have reported that M.oleifera Lam.seeds iso thiocyanate(MITC)has anticancer,anti-inflammatory,cardio metabolic repair,nervous system protection,blood lipid regulation and diabetes prevention properties.However,the molecular mechanisms of MITC with protective effects against skin photoaging have not been studied thus far.In this study,we aimed to evaluate the antiphotoaging activity of MITC and to investigate the effect of p38 MAPK-dependent autophagy in vivo and in vitro models of photoaging.In this research we found that MITC can reverse the intracellular reactive oxygen species(ROS)content and inhibit the activation of p38 MAPK to improve the autophagy level,reduce the expression of matrix metalloproteinases(MMPs),and finally protect against photoaging by UV.Our results will uncover the molecular mechanisms of MITC that play a role in the protective effects against skin photoaging,provide helpful information for developing MITC as an anti-photoaging plant material and improve the utilization of M.oleifera Lam.seeds.展开更多
Moringa oleifera Lam.is a Moringa genus in the Moringaceae family that is high in nutrients and has a wide range of applications.Phenolic compounds are widely found in plants and have various health benefits for the h...Moringa oleifera Lam.is a Moringa genus in the Moringaceae family that is high in nutrients and has a wide range of applications.Phenolic compounds are widely found in plants and have various health benefits for the human body.With its high content and wide variety of phenolic compounds,M.oleifera Lam.has been widely studied for its health benefits.The phenolic compounds in M.oleifera Lam.(MOPCs)can be a potential source of functional food ingredients in pharmaceutical and industrial applications.Numerous studies have shown that MOPCs have antioxidant,anti-obesity,anti-diabetic,and antibacterial effects.Although the research on MOPCs has been gradually increasing,the extraction,isolation,identification,biological activities,and comprehensive application of MOPCs need a more systematic summary and generalization.Therefore,this paper reviews the isolation and extraction methods,structure identification,biological activities,and comprehensive applications to provide a further reference for the research and application of MOPCs.展开更多
This study employed an integrated approach combining bioinformatics and network pharmacology to elucidate the potential active compounds and mechanisms of action of Toddalia asiatica(L.)Lam.(T.asiatica)in treating ost...This study employed an integrated approach combining bioinformatics and network pharmacology to elucidate the potential active compounds and mechanisms of action of Toddalia asiatica(L.)Lam.(T.asiatica)in treating osteoarthritis(OA).Initially,the components of T.asiatica were extracted from the literature,and their ADMF properties were assessed using SwissADME.Subsequently,the corresponding targets for the effective components were predicted through the SwissTargetPrediction database.A drug-component-target network was then constructed using Cytoscape,which facilitated the identification of the primary active components based on their degree values within the network.In parallel,differentially expressed genes related to OA were comprehensively analyzed utilizing GEO data.The intersection of the targets from T.asiatica and those associated with OA was determined,followed by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses,as well as the construction of a protein-protein interaction(PPI)network through the String platform.The topological network was visualized using Cytoscape,and core genes were identified via the Cytohubba plugin.In total,54 effective components and 694 corresponding targets of T.asiatica were predicted.Based on the degree values from the drug-component-target network,22 principal active components were ultimately identified.From the GEO database,493 OA-related targets were retrieved,revealing 46 shared targets between T.asiatica and OA.The enrichment analysis of these intersecting genes suggested that T.asiatica may exert anti-OA effects through multiple components,targets,and pathways.Notably,the PPI network analysis highlighted MCL1 as a core target.The diagnostic potential of this gene was further evaluated using an external validation set for OA.Additionally,the binding affinity of the 22 main active components of T.asiatica to MCL1 was elucidated through molecular docking studies.This research enhanced our understanding of the pharmacological effects of T.asiatica against OA and its active components,providing valuable insights and evidence for future studies.展开更多
Objective:To investigate the acute and delayed anti-inflammatory effects of Morning oleifera lam(MOL) crude methanolic extract.Methods:Compared the anti-inflammatory effects of MOL with that of standard anti-inflamm...Objective:To investigate the acute and delayed anti-inflammatory effects of Morning oleifera lam(MOL) crude methanolic extract.Methods:Compared the anti-inflammatory effects of MOL with that of standard anti-inflammatory agents like indomethacin and hydrocortisone using Air Pouch Model.Results:In both acute and delayed inflammation,the MOL extract produced dose dependent anti-inflammatory effect[acute IC50=(399.30±5.43) mg/kg;delayed IC50=(510.26±4.53) mg/kg].The order of anti-inflammatory potency for the three drugs was hydrocortisone】 indomethacin 】 MOL.Conclusions:These observations indicate that MOL possesses potential anti-inflammatory property.展开更多
基金the National Natural Science Foundation of China(31960508)Science and Technology Plan Project of Science and Technology Department of Yunnan Province(202401BD070001-034)Yunnan Province-City Integration Project(202302AN360002).
摘要Some visitors to India often experience diarrhea and attribute it to Indian food;however,murine food allergy model have shown that diarrhea occurring after consumption of Moringa may be due to an allergic reaction.The findings of this study indicate that Moringa leaf protein(Mo-Pr)has the potential to sensitize BALB/c mice.Mo-Pr has been demonstrated to stimulate the mice's production of Mo-Pr-specific immunoglobulin E,promote mast cell degranulation,and induce the release of histamine and other inflammatory mediators.Mo-Pr has been demonstrated to promote the proliferation and differentiation of Th2 cells while simultaneously inhibiting the proliferation and differentiation of Thl and Treg cells.The sera Western blot shown that the significant band Mo-Pr molecule weight is 36 kDa respectively,which was identified as fructose-1,6-bisphosphate aldolase by liquid chromatographytandem mass spectrometry.
摘要特藏资源已跻身图书馆的核心竞争力之列。晚清民国的“高龄”特藏面临严重损毁危机,传统研究缺乏从图书馆、档案馆、博物馆(Libraries,Archives and Museums,LAM)协同视角探索全流程活化路径。中国地质大学(武汉)通过机构改革,合并图书馆、档案馆、校史馆、博物馆为图书档案与文博部,形成一部辖四馆的组织架构,通过治理协同、资源协同和业务协同对具有地学特色的“高龄”特藏文献进行濒危抢救和价值创造,取得良好成效。中国地质大学(武汉)的实践表明:优化行政治理、打造文化场馆集群,强化场馆资源聚类和业务协同,不失为高校文化机构开展特藏建设的可行路径之一。
基金supported by National Natural Science Foundation of China(82260703)。
摘要People living long-term in areas with UV will cause premature photoaging.An abnormal reduction in autophagy is a key feature of photoaging,and p38 MAPK has been regarded as a key regulator of autophagy.Isothiocyanate is one of the main active components of Moringa oleifera Lam.seeds.Studies have reported that M.oleifera Lam.seeds iso thiocyanate(MITC)has anticancer,anti-inflammatory,cardio metabolic repair,nervous system protection,blood lipid regulation and diabetes prevention properties.However,the molecular mechanisms of MITC with protective effects against skin photoaging have not been studied thus far.In this study,we aimed to evaluate the antiphotoaging activity of MITC and to investigate the effect of p38 MAPK-dependent autophagy in vivo and in vitro models of photoaging.In this research we found that MITC can reverse the intracellular reactive oxygen species(ROS)content and inhibit the activation of p38 MAPK to improve the autophagy level,reduce the expression of matrix metalloproteinases(MMPs),and finally protect against photoaging by UV.Our results will uncover the molecular mechanisms of MITC that play a role in the protective effects against skin photoaging,provide helpful information for developing MITC as an anti-photoaging plant material and improve the utilization of M.oleifera Lam.seeds.
基金supported by Major Project of Science and Technology Department of Yunnan Province(202002AA100005,202102AE090027-2)National Natural Science Foundation of China(82260703)+1 种基金Cassava Industrial Technology System of China(CARS11-YNTY)Yunnan Province Ten Thousand Plan Industrial Technology Talents Project(YNWR-CYJS-2020-010)。
摘要Moringa oleifera Lam.is a Moringa genus in the Moringaceae family that is high in nutrients and has a wide range of applications.Phenolic compounds are widely found in plants and have various health benefits for the human body.With its high content and wide variety of phenolic compounds,M.oleifera Lam.has been widely studied for its health benefits.The phenolic compounds in M.oleifera Lam.(MOPCs)can be a potential source of functional food ingredients in pharmaceutical and industrial applications.Numerous studies have shown that MOPCs have antioxidant,anti-obesity,anti-diabetic,and antibacterial effects.Although the research on MOPCs has been gradually increasing,the extraction,isolation,identification,biological activities,and comprehensive application of MOPCs need a more systematic summary and generalization.Therefore,this paper reviews the isolation and extraction methods,structure identification,biological activities,and comprehensive applications to provide a further reference for the research and application of MOPCs.
基金National Natural Science Foundations of China(Grant No.81960870)Key Research and Development Program of Yunnan Science and Technology Department(Grant No.2024-03AC100019)+2 种基金Key Research and Development Program of Yunnan Science and Technology Department(Grant No.202303AC100326)Expert Workstation of zhangxuan in Yunnan Province(Grant No.202305AF150175)Education Department of Yunnan Province(Grant No.2023Y0463 and 2024Y380)。
摘要This study employed an integrated approach combining bioinformatics and network pharmacology to elucidate the potential active compounds and mechanisms of action of Toddalia asiatica(L.)Lam.(T.asiatica)in treating osteoarthritis(OA).Initially,the components of T.asiatica were extracted from the literature,and their ADMF properties were assessed using SwissADME.Subsequently,the corresponding targets for the effective components were predicted through the SwissTargetPrediction database.A drug-component-target network was then constructed using Cytoscape,which facilitated the identification of the primary active components based on their degree values within the network.In parallel,differentially expressed genes related to OA were comprehensively analyzed utilizing GEO data.The intersection of the targets from T.asiatica and those associated with OA was determined,followed by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses,as well as the construction of a protein-protein interaction(PPI)network through the String platform.The topological network was visualized using Cytoscape,and core genes were identified via the Cytohubba plugin.In total,54 effective components and 694 corresponding targets of T.asiatica were predicted.Based on the degree values from the drug-component-target network,22 principal active components were ultimately identified.From the GEO database,493 OA-related targets were retrieved,revealing 46 shared targets between T.asiatica and OA.The enrichment analysis of these intersecting genes suggested that T.asiatica may exert anti-OA effects through multiple components,targets,and pathways.Notably,the PPI network analysis highlighted MCL1 as a core target.The diagnostic potential of this gene was further evaluated using an external validation set for OA.Additionally,the binding affinity of the 22 main active components of T.asiatica to MCL1 was elucidated through molecular docking studies.This research enhanced our understanding of the pharmacological effects of T.asiatica against OA and its active components,providing valuable insights and evidence for future studies.
摘要Objective:To investigate the acute and delayed anti-inflammatory effects of Morning oleifera lam(MOL) crude methanolic extract.Methods:Compared the anti-inflammatory effects of MOL with that of standard anti-inflammatory agents like indomethacin and hydrocortisone using Air Pouch Model.Results:In both acute and delayed inflammation,the MOL extract produced dose dependent anti-inflammatory effect[acute IC50=(399.30±5.43) mg/kg;delayed IC50=(510.26±4.53) mg/kg].The order of anti-inflammatory potency for the three drugs was hydrocortisone】 indomethacin 】 MOL.Conclusions:These observations indicate that MOL possesses potential anti-inflammatory property.