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Galectin-3、sST2和GDF-15对不同射血分数心力衰竭病人的预后价值比较 认领 引用
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作者 王月祥 胡擎天 +5 位作者 曾超超 陈杰 胡司淦 耿志军 李静 刘进军 《蚌埠医科大学学报》 CAS 2026年第5期588-592,共5页
目的:探讨半乳糖凝集素-3(Galectin-3)、可溶性生长刺激表达基因2蛋白(sST2)及生长分化因子15(GDF-15)对不同射血分数的心力衰竭病人的诊断价值及预后比较。方法:选取慢性心力衰竭病人120例,根据左心室射血分数将心力衰竭病人分为3组,... 目的:探讨半乳糖凝集素-3(Galectin-3)、可溶性生长刺激表达基因2蛋白(sST2)及生长分化因子15(GDF-15)对不同射血分数的心力衰竭病人的诊断价值及预后比较。方法:选取慢性心力衰竭病人120例,根据左心室射血分数将心力衰竭病人分为3组,即射血分数保留型心衰组(HFpEF,EF≥50%)、射血分数中间型心衰组(HFmrEF,49%≥EF≥41%)、射血分数降低型心衰组(HFrEF,EF≤40%),各40例。选取同期体检健康病人33名为对照组,统计各组入选病人的一般临床资料,采用酶联免疫吸附法(ELISA)测定各组病人血清Galectin-3、sST2和GDF-15的水平。完成6个月随访后,对病人相关参数进行统计学分析。结果:对照组与心衰组在射血分数、NT-proBNP、左室舒张末径、sST2、Galectin-3、GDF-15等方面差异均有统计学意义(P<0.01)。其中射血分数在对照组、保留型心衰组、中间型心衰组、降低型心衰组之间依次降低(P<0.01),NT-proBNP、左室舒张末径、sST2、Galectin-3、GDF-15等5个指标在对照组、保留型心衰组、中间型心衰组、降低型心衰组之间均呈现依次升高(P<0.01)。Spearman相关性分析显示,心衰组中sST2、Galectin-3、GDF-15、NT-proBNP、LVDD之间呈正相关关系(P<0.01),且上述5个指标与射血分数均呈负相关关系(P<0.01)。不良预后事件ROC曲线显示,HFrEF组和HFmrEF组病人的NT-proBNP指标具有较高的AUC值、灵敏度和特异度,HFpEF病人的GDF-15指标具有较高的AUC值、灵敏度和特异度。多因素COX回归分析显示在不同类型的慢性心衰组在不同类型的心衰组中sST2(HR=0.976、95%CI=0.956~0.995)、NT-proBNP(HR=1.000、95%CI=1.000~1.001)、EF(HR=1.134、95%CI=1.060~1.214)和GDF-15(HR=1.004、95%CI=1.001~1.008)均可以作为心衰病人不良预后事件的独立预测因子(P<0.05~P<0.01)。结论:在不同类型的心衰组中sST2、Galectin-3和GDF-15对心衰病人不良事件的发生均有较高的预测价值。同时sST2、GDF-15和NT-proBNP可作为独立危险因子对病人短期风险事件进行预测评估。 展开更多
关键词 心力衰竭 半乳糖凝集素-3 可溶性生长刺激表达基因2蛋白 生长分化因子15
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The lectin-rhizobium interactions underlying symbiotic nodulation:a focal review on vegetable legumes 认领 引用
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作者 Su Yang Siyi Wang +5 位作者 Hao Chen Yuxin Gong Chenze Lu Min Xu Kang Ning Pei Xu 《Vegetable Research》 2025年第1期143-153,共11页
Lectins are carbohydrate-binding proteins that play key roles in cell recognition,signaling,and plant defense.In leguminous plants,lectins are crucial for symbiotic interactions with rhizobia—nitrogen-fixing bacteria... Lectins are carbohydrate-binding proteins that play key roles in cell recognition,signaling,and plant defense.In leguminous plants,lectins are crucial for symbiotic interactions with rhizobia—nitrogen-fixing bacteria that enhance soil fertility and promote plant growth.Understanding the regulatory networks underlying lectin-rhizobium interactions is essential for advancing agricultural biotechnology and global food security.Although substantial advances have been made in elucidating these interactions and summarized in several reviews,most presented knowledge comes from model legumes such as Medicago sativa,Lotus japonicus,and Glycine max.In contrast,vegetable legumes,characterized by their edible immature pods or seeds,occupy a significant position in global agriculture,yet their lectin-rhizobium interactions remain poorly summarized.To address this gap,this review explores the intricate mechanisms governing lectin-rhizobium interactions,with a particular emphasis on insights derived from vegetable legumes such as common beans,cowpeas,and peas.Following the introduction of lectins and rhizobia,the complete process of symbiotic nitrogen fixation is revisited,spanning from mutual recognition mechanism between lectins and rhizobia to nodule formation and nitrogen fixation.The potential of transferring legume lectin genes into non-leguminous crops to improve nitrogen fixation is also discussed.Finally,the importance of unraveling the molecular mechanisms governing these interactions is highlighted to enhance symbiotic efficiency and promote sustainable crop production. 展开更多
关键词 nitrogen fixation agricultural biotechnology rhizobia enhance soil fertility symbiotic nodulation vegetable legumes lectins symbiotic interactions
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β-Glucan pretreatment activates lectin pathway to maintain the function of intestinal Th17 cells for infectious enteritis protection 认领 引用
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作者 Yanbo Zhao Xiaochuan Mei +4 位作者 Jin Yang Zhuang Wang Qin Liu Yuanxing Zhang Dahai Yang 《Zoological Research》 SCIE CAS CSCD 2025年第6期1411-1424,共14页
Enteritis,involving inflammation of the small intestine,is often accompanied by immune cell dysfunction during intestinal infections.Immunomodulatory β-glucans(BGs)have recently been shown to support antibacterial im... Enteritis,involving inflammation of the small intestine,is often accompanied by immune cell dysfunction during intestinal infections.Immunomodulatory β-glucans(BGs)have recently been shown to support antibacterial immune responses through the induction of trained immunity.However,little is known about the potential role of BG pretreatment in protecting against infectious enteritis in teleost fish.In this work,by establishing an adult zebrafish enteritis model via infection with the fish pathogen Edwardsiella piscicida and pretreating it with BGs,we demonstrated that such pretreatment confers protection against infectious enteritis,accompanied by reduced production of proinflammatory cytokines.Specifically,we found that BG pretreatment could amplify intestinal lectin pathway-associated complement activation to ameliorate the infectious enteritis.Moreover,through comprehensive RNA-seq analysis of immune cell marker genes in zebrafish,we revealed that the lectin pathway amplification by BG pretreatment modulated the responsiveness of intestinal Th17 cells,which was essential for the protection against infectious enteritis.Collectively,these findings identify the intestinal lectin pathway as a potential mediator of the effects of BG pretreatment and reveal its role in maintaining the function of Th17 cells in zebrafish.This suggests that harnessing BG-induced trained immunity might represent a promising therapeutic strategy against infectious enteritis in teleost. 展开更多
关键词 β-Glucan Lectin pathway Th17 cell Edwardsiella piscicida Infectious enteritis Zebrafish
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Use of lectin microarray to differentiate gastric cancer from gastric ulcer 认领 引用 被引量：3
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作者 Wei-Li Huang Yang-Guang Li +3 位作者 Yong-Chen Lv Xiao-Hui Guan Hui-Fan Ji Bao-Rong Chi 《World Journal of Gastroenterology》 SCIE CAS 2014年第18期5474-5482,共9页
AIM: To investigate the feasibility of lectin microarray for differentiating gastric cancer from gastric ulcer. METHODS: Twenty cases of human gastric cancer tissue and 20 cases of human gastric ulcer tissue were coll... AIM: To investigate the feasibility of lectin microarray for differentiating gastric cancer from gastric ulcer. METHODS: Twenty cases of human gastric cancer tissue and 20 cases of human gastric ulcer tissue were collected and processed. Protein was extracted from the frozen tissues and stored. The lectins were dissolved in buffer, and the sugar-binding specificities of lectins and the layout of the lectin microarray were summarized. The median of the effective data points for each lectin was globally normalized to the sum of medians of all effective data points for each lectin in one block. Formalin-fixed paraffin-embedded gastric cancer tissues and their corresponding gastric ulcer tissues were subjected to Ag retrieval. Biotinylated lectin was used as the primary antibody and HRP-streptavidin as the secondary antibody. The glycopatterns of glycoprotein in gastric cancer and gastric ulcer specimens were determined by lectin microarray, and then validated by lectin histochemistry. Data are presented as mean +/- SD for the indicated number of independent experiments. RESULTS: The glycosylation level of gastric cancer was significantly higher than that in ulcer. In gastric cancer, most of the lectin binders showed positive signals and the intensity of the signals was stronger, whereas the opposite was the case for ulcers. Significant differences in the pathological score of the two lectins were apparent between ulcer and gastric cancer tissues using the same lectin. For MPL and VVA, all types of gastric cancer detected showed stronger staining and a higher positive rate in comparison with ulcer, especially in the case of signet ring cell carcinoma and intra-mucosal carcinoma. GalNAc bound to MPL showed a significant increase. A statistically significant association between MPL and gastric cancer was observed. As with MPL, there were significant differences in VVA staining between gastric cancer and ulcer. CONCLUSION: Lectin microarray can differentiate the different glycopatterns in gastric cancer and gastric ulcer, and the lectins MPL and VVA can be used as biomarkers. (C) 2014 Baishideng Publishing Group Co., Limited. All rights reserved. 展开更多
关键词 Gastric cancer Gastric ulcer Lectin microarray Lectin histochemistry Differentiate
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Extraction of Six Seaweed Lectins and Comparison of Their Lectin Activity 认领 引用
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作者 CONG Yu-ting WANG Li +2 位作者 LU Ya-nan HU Xin-jiang MA Guo-qing 《Agricultural Science & Technology》 CAS 2019年第3期26-30,共5页
Seaweeds are marine biological resources rich in a variety of bioactive substances with a wide range of biological functions.In this research,lectins were extracted from C.ocellatus Holmes,Rhodomela C.Ag,Laminaria jap... Seaweeds are marine biological resources rich in a variety of bioactive substances with a wide range of biological functions.In this research,lectins were extracted from C.ocellatus Holmes,Rhodomela C.Ag,Laminaria japonica Aresch,Undaria pinnati fida,Porphyra yezoensis Ueda and Alga Scytosiphonis Lomentarii.The activities of the lectins were measured and analyzed respectively.The results showed that the effect of extracting the lectin with normal saline was better,and the activity in kelp crude extract was better.It can provide a reference for further development and utilization of economic seaweed. 展开更多
关键词 Seaweed Lectin Lectin activity
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Mannose-binding lectin and maladies of the bowel and liver 认领 引用 被引量：3
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作者 Daniel L Worthley Peter G Bardy +1 位作者 David L Gordon Charles G Mullighan 《World Journal of Gastroenterology》 SCIE CAS 2006年第40期6420-6428,共9页
Mannose-binding lectin (MBL) is a pattern-recognition molecule that binds to characteristic carbohydrate mo-tifs present on the surface of many different pathogens. MBL binding stimulates the immune system via the lec... Mannose-binding lectin (MBL) is a pattern-recognition molecule that binds to characteristic carbohydrate mo-tifs present on the surface of many different pathogens. MBL binding stimulates the immune system via the lectin pathway of complement activation. In certain clinical situations, often characterized by pre-existing immune compromise, MBL deficiency increases the risk of infec-tious and other disease-specific complications. Many of the key pathogenic processes inherent to common gastroenterological diseases, such as infection, immuno-logical damage, and carcinogenesis, have been linked to MBL. This editorial reviews the biology of MBL, outlines key disease associations to document the breadth of influence of MBL, and finally, highlights the relevance of MBL to both gastroenterological health and disease. 展开更多
关键词 Mannose-binding lectin Collectins, Innate immunity Polymorphism Infection
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Mechanisms involved in Korean mistletoe lectin-induced apoptosis of cancer cells 认领 引用 被引量：7
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作者 Lee-Yong Khil Wi Kim +3 位作者 Suyun Lyu Won Bong Park Ji-Won Yoon Hee-Sook Jun 《World Journal of Gastroenterology》 SCIE CAS 2007年第20期2811-2818,共8页
AIM： To investigate the anti-cancer mechanisms of Korean mistletoe lectin （Viscum album coloratura agglutinin, VCA） using a human colon cancer cell line （COLO）. METHODS： Cytotoxic effects of VCA on COLO cells we... AIM： To investigate the anti-cancer mechanisms of Korean mistletoe lectin （Viscum album coloratura agglutinin, VCA） using a human colon cancer cell line （COLO）. METHODS： Cytotoxic effects of VCA on COLO cells were determined by 3-（4,5-dimethylthiazol-2-yl）-2,5- diphenyltetrazolium bromide （MTI-） assay in vitro and tumor-killing effects in vivo. To study the mechanisms involved, the expression of various pro-caspases, anti- apoptotic proteins, and death receptors was determined by western blot. To determine which death receptor is involved in VCA-induced apoptosis of COLO cells, cytotoxicity was examined by MTT assay after treatment with agonists or antagonists of death receptors. RESULTS： VCA killed COLO cells in a time- and dosedependent manner and induced complete regression of tumors in nude mice transplanted with COLO cells. Treatment of COLO cells with VCA activated caspase-2, -3, -8, and -9 and decreased expression of anti-apoptotic molecules including receptor interacting protein, nuclear factor-κB, X-linked inhibitor of apoptosis protein, and Akt/protein kinase B. We then examined the involvement of death receptors in VCA-induced apoptosis. Only tumor necrosis factor receptor 1, among the death receptors examined, was involved in apoptosis of COLO cells, evidenced by inhibition of VCA-induced apoptosis and decreased activation of caspases, particularly caspase-8, by tumor necrosis factor receptor 1 antagonizing antibody.CONCLUSION： VCA-induced apoptotic COLO cell death is due to the activation of caspases and inhibition of antiapoptotic proteins, in part through the tumor necrosis factor receptor 1 signaling pathway. 展开更多
关键词 Viscum album coloratum Lectin Apoptosis Tumor necrosis factor receptor-i Caspase Colon cancer
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Pancreatitis-associated protein:From a lectin to an anti-inflammatory cytokine 认领 引用 被引量：8
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作者 Daniel Closa Yoshiharu Motoo Juan L Iovanna 《World Journal of Gastroenterology》 SCIE CAS 2007年第2期170-174,共5页
Pancreatitis-associated protein (PAP) was discovered in the pancreatic juice of rats with acute pancreatitis. PAP is a 16 kDa secretory protein structurally related to the C-type lectins although classical lectin-rela... Pancreatitis-associated protein (PAP) was discovered in the pancreatic juice of rats with acute pancreatitis. PAP is a 16 kDa secretory protein structurally related to the C-type lectins although classical lectin-related function has not been reported yet. Then, it was demonstrated that PAP expression may be activated in some tissues in a constitutive or injury- and inflammation-induced manner. More recently, it has been found that PAP acts as an anti-inflammatory factor in vitro and in vivo. PAP expression can be induced by several pro- and anti-inflammatory cytokines and by itself through a JAK/STAT3-dependent pathway. PAP is able to activate the expression of the anti-inflammatory factor SOCS3 through the JAK/STAT3-dependent pathway. The JAK/STAT3/SOCS3 pathway seems to be a common point between PAP and several cytokines. Therefore, it is reasonable to propose that PAP is a new anti- inflammatory cytokine. 展开更多
关键词 Pancreatitis-associated protein Pancreatitis .1anus kinases STAT3 SOCS3 Anti-inflammatory Lectin
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A novel lectin from mushroom Phellodon melaleucus displays hemagglutination activity,and antitumor activity in a B16 melanoma mouse model 认领 引用 被引量：4
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作者 Yuanhui Li Peng Wang +1 位作者 Zejun Zhang Qinghong Liu 《Food Science and Human Wellness》 SCIE CAS CSCD 2023年第5期1885-1892,共8页
A novel lectin(termed PML)was purified from fruiting bodies of the edible mushroom Phellodon melaleucus(division Basidiomycota)by ion exchange,hydrophobic interaction,and gel filtration chromatographies,with overall t... A novel lectin(termed PML)was purified from fruiting bodies of the edible mushroom Phellodon melaleucus(division Basidiomycota)by ion exchange,hydrophobic interaction,and gel filtration chromatographies,with overall titer recovery~60%and 20-fold purification.PML displayed hemagglutination activity 13319 units/mg toward rabbit erythrocytes.SDS-PAGE and gel filtration analyses revealed that PML is a homodimeric lectin with a molecular weight of 28.8 kDa.PML hemagglutination activity was not inhibited by various simple sugars or their derivatives,but was enhanced by cations Ca2+,Mg2+,Zn2+,and Cu2+.The activity was stable in pH range 6–9 and in the temperature range 20–60°C.Circular dichroism(CD)spectroscopic analysis showed that PML was composed primarily ofβ-sheets with lowα-helix content.In a B16 melanoma mouse model,PML treatment significantly inhibited tumor growth,and increased cytokine IL-10 content.Our findings suggest that PML is a potential anticancer therapeutic agent. 展开更多
关键词 Phellodon melaleucus Lectin Hemagglutination activity Cytokine IL-10 Antitumor activity
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非洲猪瘟病毒C-type lectin蛋白的原核表达及其免疫特性研究 认领 引用 被引量：2
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作者 谢振华 刘英楠 +6 位作者 张萌 敖清莹 吕璐 于婉琪 扈荣良 邬静 陈鸿军 《中国兽医科学》 CAS CSCD 北大核心 2021年第1期37-44,共8页
为了制备非洲猪瘟病毒(ASFV)SY18毒株的C-type lectin蛋白多抗,以基因序列优化的真核质粒为模板,扩增EP153R基因,构建原核表达重组质粒pET-32a-EP153R。将重组质粒转化大肠杆菌BL21(DE3)感受态中,利用IPTG诱导宿主菌表达后,纯化C-type l... 为了制备非洲猪瘟病毒(ASFV)SY18毒株的C-type lectin蛋白多抗,以基因序列优化的真核质粒为模板,扩增EP153R基因,构建原核表达重组质粒pET-32a-EP153R。将重组质粒转化大肠杆菌BL21(DE3)感受态中,利用IPTG诱导宿主菌表达后,纯化C-type lectin重组蛋白。用纯化蛋白免疫BALB/c小鼠,制备C-type lectin蛋白多抗,利用Western-blot和间接免疫荧光技术鉴定该抗体的反应性和特异性。结果表明,原核表达重组蛋白C-type lectin经IPTG诱导表达成功,大小约为38 ku,其主要以包涵体形式表达,且能与His标签单抗发生反应,C-type lectin蛋白多抗可特异性识别非洲猪瘟病毒C-type lectin蛋白。本研究为非洲猪瘟血清学检测方法的建立及C-type lectin蛋白生物学功能的探究奠定了基础。 展开更多
关键词 非洲猪瘟病毒 C-type lectin蛋白 抗体 免疫特性
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LPS诱导小鼠脑小胶质细胞的激活及Tomato lectin的表达变化 认领 引用 被引量：3
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作者 邓小华 蔡维君 +3 位作者 李昌琪 代玉桥 雷德亮 罗学港 《神经解剖学杂志》 CAS 北大核心 2006年第6期634-638,共5页
探讨小胶质细胞对侧脑室注射内毒素脂多糖(lipopolysaccharide,LPS)刺激的反应及时程变化。采用组织化学方法,观察小鼠单次注射LPS入侧脑室后,Tomatolectin阳性的小胶质细胞于不同时间点在脑内的分布及表达的时程变化。LPS注射24h后,Tom... 探讨小胶质细胞对侧脑室注射内毒素脂多糖(lipopolysaccharide,LPS)刺激的反应及时程变化。采用组织化学方法,观察小鼠单次注射LPS入侧脑室后,Tomatolectin阳性的小胶质细胞于不同时间点在脑内的分布及表达的时程变化。LPS注射24h后,Tomatolectin组织化学染色显示血管内皮细胞、静息的和不同激活状态的小胶质细胞均呈阳性反应,其中Tomatolectin阳性的小胶质细胞明显被激活,2d时达表达高峰,巨噬细胞样的细胞和小胶质细胞呈现出肥大的、阿米巴样或杆状细胞等不同激活状态下的形态特征。这些激活的小胶质细胞主要分布于侧脑室周围的脑实质结构,如海马、隔区、胼胝体、纹状体,第三脑室周围的下丘脑及其它间脑结构。本结果提示:LPS能诱导小鼠脑室周围的脑实质内的小胶质细胞被激活,并上调小胶质细胞内Toma-tolectin的表达,这些细胞可能参与脑内刺激的免疫调节。 展开更多
关键词 脂多糖 Tomato lectin 免疫组织化学 小胶质细胞小鼠
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Functional identification of C-type lectin in the diamondback moth,Plutella xylostella(L.)innate immunity 认领 引用 被引量：2
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作者 LI Jin-yang LIN Jun-han +3 位作者 G.Mandela FERNANDEZ-GRANDON ZHANG Jia-yu YOU Min-sheng XIA Xiao-feng 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2021年第12期3240-3255,共16页
C-type lectins(CTLs)are a superfamily of Ca2+-dependent carbohydrate-recognition proteins,and an important pattern recognition receptor(PRR)in insect innate immunity which can mediate humoral and cellular immunity ... C-type lectins(CTLs)are a superfamily of Ca2+-dependent carbohydrate-recognition proteins,and an important pattern recognition receptor(PRR)in insect innate immunity which can mediate humoral and cellular immunity in insects.In this study,we report a novel dual carbohydrate-recognition domain(CRD)CTL from Plutella xylostella which we designate PxIML.PxIML is a protein with a 969 bp open reading frame(ORF)encoding 322 amino acids,containing a signal peptide and a dual-CRD with EPN(Glu124-Pro125-Asn126)and QPD(Gln274-Pro275-Asp276)motifs.The expression of PxIML mRNA in the fat body was significantly higher than in hemocytes and midgut.The relative expression levels of PxIML in the whole insect and the fat body were significantly inhibited after infection with Bacillus thuringiensis 8010(Bt8010)at 18 h,while they were significantly upregulated after infection with Serratia marcescens IAE6 or Pichia pastoris.The recombinant PxIML(rPxIML)protein could bind to the tested pathogen-associated molecular patterns(PAMPs),and the bacteria of Enterobacter sp.IAE5,S.marcescens IAE6,Staphylococcus aureus,Escherichia coli BL21,and Bt8010 in a Ca2+-dependent manner,however,it showed limited binding to the fungus,P.pastoris.The rPxIML exhibited strong activity in the presence of Ca2+ to agglutinate Bt8010,Enterobacter sp.IAE5 and S.aureus,but it only weakly agglutinated with E.coli BL21,and could not agglutinate with S.marcescens IAE6 or P.pastoris.Furthermore,the rPxIML could bind to hemocytes,promote the adsorption of hemocytes to beads,and enhance the phenoloxidase(PO)activity and melanization of P.xylostella.Our results suggest that PxIML plays an important role in pathogen recognition and in mediating subsequent humoral and cellular immunity of P.xylostella. 展开更多
关键词 diamondback moth humoral and cellular immunity C-type lectin pattern recognition receptors carbohydrate-recognition domain
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Research on the Lectin in Muscle Tissue of Varicorhinus macrolepis 认领 引用 被引量：4
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作者 庞秋香 赵先锋 +2 位作者 孙欢欢 赵博生 孙皓 《Agricultural Science & Technology》 CAS 2011年第10期1535-1538,共4页
[Objective] The paper aimed at researching lectins in muscles of Varicorhinus macrolepis and providing scientific basis for researching the adaptation mechanism and immune response of V.macrolepis to environment,which... [Objective] The paper aimed at researching lectins in muscles of Varicorhinus macrolepis and providing scientific basis for researching the adaptation mechanism and immune response of V.macrolepis to environment,which were advantageous for the protection and reproduction of V.macrolepis.[Method] V.macrolepis was used as test materials for the hemagglutination test by dialdehyde fixation to prove the existence of lectins in muscle crude homogenate of Varicorhinus macrolepis and study the physical and chemical characters.[Result] Lectins in muscle crude homogenate of V.macrolepis had shown hemagglutination effects on erythrocytes of six types of animals and had the maximum hemagglutination activity against rabbit erythrocytes,which belonged to the S-type lectins with optimal pH ranged from 4 to 8 and optimal temperature at 60 ℃.Results from the saccharide inhibition test had indicated that the sucrose was the only kind of saccharide which had inhibited the hemagglutination,suggesting that sucrose had played an important role in the process of recognition and aggregation of lectins.[Conclusion] It had been speculated that the optimal pH ranges for thermal sensitivity and hemagglutination activity of lectins in different types of aquatic organisms were similar. 展开更多
关键词 V.macrolepis Muscle crude homogenate Lectin Dialdehyde fixation Hemagglutination activity
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Lectin法分析uNK细胞在人妊娠母胎界面的分布变化 认领 引用 被引量：1
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作者 钟婷 宗滕 +3 位作者 张鹏 张保平 彭婧 况海斌 《南昌大学学报（理科版）》 CAS 北大核心 2013年第3期267-271,共5页
通过Lectin法分析uNK细胞在人妊娠早、晚期母胎界面的分布变化。结果发现:(1)在妊娠早期的蜕膜中,uNK细胞主要有两种形式,一种是成熟uNK细胞,细胞体积较大,胞浆和胞膜呈现强烈的棕黄色染色,分布于蜕膜的血管中。另一种是未成熟uNK细胞,... 通过Lectin法分析uNK细胞在人妊娠早、晚期母胎界面的分布变化。结果发现:(1)在妊娠早期的蜕膜中,uNK细胞主要有两种形式,一种是成熟uNK细胞,细胞体积较大,胞浆和胞膜呈现强烈的棕黄色染色,分布于蜕膜的血管中。另一种是未成熟uNK细胞,细胞体积较小,胞核呈现黄色染色,分布于蜕膜细胞之间。(2)在妊娠早期的绒毛组织中,uNK主要是未成熟uNK,分布于绒毛间质之间。(3)与妊娠早期蜕膜的uNK细胞数相比,至妊娠晚期,蜕膜的uNK细胞数量明显减少(P<0.01)。(4)在妊娠晚期,绒毛组织不表达uNK细胞。结论:uNK细胞主要分布于子宫蜕膜组织,且随着妊娠的进展,其表达数量明显下降。这种时空性的表达变化可能与其在调控滋养层细胞的侵袭、血管重建及免疫耐受方面密切相关。 展开更多
关键词 uNK细胞 Lectin染色 子宫 母胎界面 妊娠
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转基因大豆GTS 40-3-2产品加工过程中lectin和cp4 epsps成分降解规律研究进展 认领 引用 被引量：1
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作者 杜艳 陈复生 +3 位作者 夏义苗 刘昆仑 张丽芬 刘伯业 《河南工业大学学报（自然科学版）》 CAS 北大核心 2019年第6期107-115,共9页
自转基因作物诞生以来,对其质疑之声从未间断。GTS 40-3-2是最早获批、应用最广泛的转基因大豆,为了更好地保护消费者知情权,国内外学者研究了不同大豆产品的加工工艺,探讨了转基因成分的变化规律,其关注点主要在转基因大豆种子检测方... 自转基因作物诞生以来,对其质疑之声从未间断。GTS 40-3-2是最早获批、应用最广泛的转基因大豆,为了更好地保护消费者知情权,国内外学者研究了不同大豆产品的加工工艺,探讨了转基因成分的变化规律,其关注点主要在转基因大豆种子检测方法的开发应用和食品(如豆浆、豆腐等)加工过程中外源成分分子量、含量的变化等方面,而关于食品加工过程中转基因成分的降解机理及本质的影响因素涉及较少。从物理、化学、生物作用3个方面对前人的研究进行梳理,分析转基因大豆GTS 40-3-2中 lectin 和 cp 4 epsps 基因降解的影响因素、降解规律,旨在找到减少甚至去除转基因成分的有效方式,为转基因大豆制品的检测和生产调控提供科学依据。 展开更多
关键词 转基因大豆 GTS 40 3 2 lectin 基因 cp4 epsps 基因 降解
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CsTs,a C-type lectin receptor-like kinase,regulates the development trichome development and cuticle metabolism in cucumber(Cucumis sativus) 认领 引用 被引量：1
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作者 Duo Lv HaiFan Wen +10 位作者 Gang Wang Juan Liu ChunLi Guo Jingxian Sun Keyan Zhang ChaoHan Li Jiaqi You Ming Pan Huanle He Run Cai Junsong Pan 《Horticulture Research》 SCIE CSCD 2024年第10期251-262,共12页
Cucumber(Cucumis sativus)fruit spines are a classic material for researching the development of multicellular trichomes.Some key genes that influence trichome development have been confirmed to be associated with cuti... Cucumber(Cucumis sativus)fruit spines are a classic material for researching the development of multicellular trichomes.Some key genes that influence trichome development have been confirmed to be associated with cuticle biosynthesis and secondary metabolism.However,the biological mechanisms underlying trichome development,cuticle biosynthesis,and secondary metabolism in cucumber remain poorly understood.CsTs,a C-type lectin receptor-like kinase gene,reportedly causes a tender trichome phenotype in cucumber when it mutates.In this study,the role of CsTs in cucumber fruit spines morphogenesis was confirmed using gene editing technology.Sectioning and cell wall component detection were used to analyse the main reason of tender fruit spines in the ts mutant.Subsequently,transcriptome data and a series of molecular biology experiments were used to further investigate the relationship between CsTs and cytoskeletal homeostasis in cucumber.CsTs overexpression partially compensated for the abnormal trichome phenotype of an Arabidopsis homolog mutant.Genetic hybridization and metabolic analysis indicated that CsTs and CsMict can affect trichome development and cuticle biosynthesis in the same pathway.Our findings provide important background information for further researching on the molecular mechanism underlying cucumber trichome development and contribute to understanding the biological function of C-type lectin receptor-like kinases. 展开更多
关键词 C type lectin receptor kinase development multicellular trichomessome secondary metabolism cucumber cuticle biosynthesis CSTS secondary metabolismhoweverthe Cucumis sativus
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Expressions and clinical significances of mannose-binding lectin(MBL) and MBL-associated serine protease 2(MASP-2) in patients with thyroid neoplasm 认领 引用 被引量：2
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作者 Yanping Shi Geling Liu +5 位作者 Huiqin Zhang Fang Yu Xiuxiu Xiang Yifang Lu Xiaomei Dong Xiaohua Li 《The Chinese-German Journal of Clinical Oncology》 2013年第3期106-108,共3页
Objective： The aim of the study was to detect the levels of mannose-binding lectin （MBL）, MBL-associated serine protease 2 （MASP-2） and explore the clinical significances of them in patients with primary thyroid ... Objective： The aim of the study was to detect the levels of mannose-binding lectin （MBL）, MBL-associated serine protease 2 （MASP-2） and explore the clinical significances of them in patients with primary thyroid neoplasms. Methods： By using ELISA method, we detected the serum levels of MBL and MASP-2 in 26 patients with papillary thyroid carcinoma （PTC）, 30 patients with thyroid adenoma （TA） and 26 healthy people, respectively. Results： Serum MBL level was （565.23 ± 76.70） μg/L in PTCs higher than （324.267 ±24.74） μg/L in TAs, and （152.69± 16.95） IJg/L in healthy of controlling group. There was statistical significance between PTC and TA （P 〈 0.05）, however there was no difference between TA and healthy （P 〉 0.05）. Serum MASP-2 level was （726.153± 78.88） pg/L in PTCs higher than （379.266 ± 30.26） μg/L in TAs, and （203.846 ± 29.09） μg/L in healthy. Serum MASP-2 level was higher in PTCs than TAs, and the difference had statistical significance （P 〈 0.01）. But no difference was observed between in TAs and healthy. Conclusion： These findings might reflect inflammatory processes induced by defense mechanisms, in response to the development of the turnout. MBL may also be involved in the elimination of possible tumourigenic pathogens. 展开更多
关键词 thyroid neoplasm mannose-binding lectin （MBL） MBL-associated serine protease 2 （MASP-2） detection
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Establishment of a Multiplex Detection Method for Common Bacteria in Blood Based on Human Mannan-Binding Lectin Protein-Conjugated Magnetic Bead Enrichment Combined with Recombinase-Aided PCR Technology 认领 引用 被引量：1
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作者 ZHAO Zi Jin CHEN Xiao Ping +13 位作者 HUA Shao Wei LI Feng Yu ZHAO Meng XING Chen Hao WANG Jie TIAN Feng Yu ZHANG Rui Qing LYU Xiao Na HAN Zhi Qiang WANG Yu Xin LI Hong Yi SHEN Xin Xin MA Xue Jun TIE Yan Qing 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2024年第4期387-398,共12页
Objective Recombinase-aided polymerase chain reaction(RAP)is a sensitive,single-tube,two-stage nucleic acid amplification method.This study aimed to develop an assay that can be used for the early diagnosis of three t... Objective Recombinase-aided polymerase chain reaction(RAP)is a sensitive,single-tube,two-stage nucleic acid amplification method.This study aimed to develop an assay that can be used for the early diagnosis of three types of bacteremia caused by Staphylococcus aureus(SA),Pseudomonas aeruginosa(PA),and Acinetobacter baumannii(AB)in the bloodstream based on recombinant human mannanbinding lectin protein(M1 protein)-conjugated magnetic bead(M1 bead)enrichment of pathogens combined with RAP.Methods Recombinant plasmids were used to evaluate the assay sensitivity.Common blood influenza bacteria were used for the specific detection.Simulated and clinical plasma samples were enriched with M1 beads and then subjected to multiple recombinase-aided PCR(M-RAP)and quantitative PCR(qPCR)assays.Kappa analysis was used to evaluate the consistency between the two assays.Results The M-RAP method had sensitivity rates of 1,10,and 1 copies/μL for the detection of SA,PA,and AB plasmids,respectively,without cross-reaction to other bacterial species.The M-RAP assay obtained results for<10 CFU/mL pathogens in the blood within 4 h,with higher sensitivity than qPCR.M-RAP and qPCR for SA,PA,and AB yielded Kappa values of 0.839,0.815,and 0.856,respectively(P<0.05).Conclusion An M-RAP assay for SA,PA,and AB in blood samples utilizing M1 bead enrichment has been developed and can be potentially used for the early detection of bacteremia. 展开更多
关键词 Staphylococcus aureus Pseudomonas aeruginosa Acinetobacter baumannii Human Mannan-binding lectin protein Bloodstream infection Recombinase-aided PCR assay Multiple detection
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Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection 认领 引用 被引量：1
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作者 罗展 张继泉 +3 位作者 李富花 张晓军 柳承璋 相建海 《Chinese Journal of Oceanology and Limnology》 CSCD 2011年第5期942-951,共10页
Acting as one of the pattern recognition receptors （PRRs）, C-type lectin is believed to mediate pathogen recognition and plays an important role in the clearance of pathogens as part of the innate immune system. In ... Acting as one of the pattern recognition receptors （PRRs）, C-type lectin is believed to mediate pathogen recognition and plays an important role in the clearance of pathogens as part of the innate immune system. In this work, a novel C-type lectin gene （named LvLecl） was cloned from the shrimp Litopenaeus vannamei, The ORF of LvLecl is 510 bp, encoding 169 amino acids. The deduced amino acid sequence contains a putative signal peptide of 19 amino acids at the N-terminal and a carbohydrate recognition domain （CRD） at the C-terminal. LvLecl was mainly expressed in the hepatopancreas. Real-time PCR analysis indicated that the level of LvLecl transcripts significantly changed in the hepatopancreas after the shrimp were artificially challenged with LPS, Micrococcus lysodeikticus and white spot syndrome virus （WSSV）. RNAi-based silencing of LvLecl resulted in increases in mortality when the shrimp were challenged with WSSV, and the median lethal time was reduced compared with controls. Although there was no characteristic ＂EPN＂ （Glu-Pro-Ser） or ＂QPD＂ （Gin-Pro-Asp） motif, the recombinant LvLecl, expressed in Escherichia coli BL21 （DE3）, could also agglutinate M. lysodeikticus and Vibrio anguillarum. The agglutinating activities were calcium-dependent and could be inhibited by D-mannose, D-glucose, D-galactose and N-Acetyl-D-mannose. These results suggest that LvLecl might be involved in the immune response against WSSV and bacterial infections and contribute to non-self recognition as a pattern recognition receptor in the innate immune system of the shrimp L. vannamei. 展开更多
关键词 Litopenaeus vannamei innate immune pattern recognition receptor C-type lectin RNAinterference （RNAi） recombinant expression
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Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides 认领 引用 被引量：1
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作者 牛建峰 王广策 +2 位作者 吕芳 周百成 彭光 《Chinese Journal of Oceanology and Limnology》 2009年第3期502-512,共11页
A group of coenocytic marine algae differs from higher plants,whose totipotency depends on an intact cell(or protoplast).Instead,this alga is able to aggregate its extruded protoplasm in sea water and generate new mat... A group of coenocytic marine algae differs from higher plants,whose totipotency depends on an intact cell(or protoplast).Instead,this alga is able to aggregate its extruded protoplasm in sea water and generate new mature individuals.It is thought that lectins play a key role in the aggregation process.We purified a lectin associated with the aggregation of cell organelles in Bryopsis hypnoides.The lectin was ca.27 kDa with a pI between pH 5 and pH 6.The absence of carbohydrate suggested that the lectin was not a glycoprotein.The hemagglutinating activity(HA) of the lectin was not dependent on the presence of divalent cations and was inhibited by N-Acetylgalactosamine,N-Acetylglucosamine,and the glycoprotein bovine submaxillary mucin.The lectin preferentially agglutinated Gram-negative bacterium.The HA of this lectin was stable between pH 4 to pH 10.Cell organelles outside the cytoplasm were agglutinated by the addition of lectin solution(0.5 mg ml-1).Our results suggest that the regeneration of B.hypnoides is mediated by this lectin.We also demonstrated that the formation of cell organelle aggregates was inhibited by nigericin in natural seawater(pH 8.0).Given that nigericin dissipates proton gradients across the membrane,we hypothesize that the aggregation of cell organelles was proton-gradient dependent. 展开更多
关键词 Bryopsis hypnoides lectin hemagglutinating cell organelle aggregation regeneration
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