The purpose of this paper was to study the effects of different C/N ratios on the maturity and microbial quantity of com-posting with sesame meal and rice straw biochar.Rice straw was calcined into biochar as raw mate...The purpose of this paper was to study the effects of different C/N ratios on the maturity and microbial quantity of com-posting with sesame meal and rice straw biochar.Rice straw was calcined into biochar as raw materials composting with sesame meal for 30 days,referring to Chenfu Agricultural Book in Sōuthern Song Dynasty(1127-1279 A.D.).Sesame meal was used to adjust the C/N ratio of compost,and three treatments were designed in the experiment,which were C/N ratios of 15,20 and 30,respectively.The results showed that C/N ratio of 20 was beneficial for promoting the temperature rise,the removal of water,the degradation of organic carbon,and the decrease of microbial quantity.The C/N ratio of 20 was beneficial to the compost maturity(T value was 0.47,final GI was 99.67%).The results of Pearson correlation analysis showed that C/N ratio was positively correlated with moisture content,total organic carbon and negatively correlated with germination index,indicating that high C/N ratio was beneficial to water removal and total organic carbon degradation.Therefore,we suggest that the suitable C/N ratio of rice straw biochar and sesame meal is 20.At the same time,we have proved that the composting method in Southern Song Dynasty is feasible,which is of great significance to understand the development of composting in China.展开更多
Mechanical construction will put influence on the biological characters of reclaimed soils,as well as the soil quality.In order to explore the changing rule of soil microbial quantity and respiratory capacity under di...Mechanical construction will put influence on the biological characters of reclaimed soils,as well as the soil quality.In order to explore the changing rule of soil microbial quantity and respiratory capacity under different construction machineries and rolling times,and find the optimal processing conditions,an experiment was set up and a simulation experimental area was chosen,in which we simulated the main types of reclamation in coal mine area.After 2 years’natural aging,we collected surface soil samples(0-20 cm)that can be used for experimental analysis.The result shows that changing rules of soil biological factors are different with different construction machineries,and soil properties are closest to the normal soil when adopting the combination of“crawler dozer×5 compaction times”and“dump truck×3 compaction times”,which shows that the soil quality is better under this condition.展开更多
To investig ate the in fluen ce of bensulfuron-methyl(BSM)on culturable microbial quantities and un cul turable microbial community structures,conventional and molecularbiolo gical meth ods were cmplo yed in five BSM ...To investig ate the in fluen ce of bensulfuron-methyl(BSM)on culturable microbial quantities and un cul turable microbial community structures,conventional and molecularbiolo gical meth ods were cmplo yed in five BSM treated soils with threc replications,respectively.The results obtained with traditional culture-dependent methods sh owed that a low-level of BSM had slight and trans ient cffects on culturable microorganisms;nevertheless,high concentration of BSM resulted in a dramatic decrease in bacterial colony forming units(cfus).The result obtained using denaturing gradient gel electrophoresis(DGGE)revealed that more than 17 bands were observed in Iow BSM contaminated soil samples and only IO bands were detected in samples with high BSM contamination.In other word s,the diversity of soil community structure is related to the concentration of BSM.Cluster analysis sho wed that the community structure und cr low lev el of con tamination was more similar to that of the control,while heavy contamin ated amendments were far aw ay from the above group.In a sen se,the cooperation of the tradition al method and the molecular biological method is more powerful to study the soil microbial information in contamin ated ccos ys tem.展开更多
[Objective] To provide a reference for exploring the relationship between Cd contamination and sugarcane growth and between Cd contamination and micro- bial properties of soil, the effects of adding different concentr...[Objective] To provide a reference for exploring the relationship between Cd contamination and sugarcane growth and between Cd contamination and micro- bial properties of soil, the effects of adding different concentrations of exogenous cadmium (Cd) on the growth of sugarcane, the quantities of soil microorganisms and the activity of soil enzymes were studied. [Method] The plant height, stem di- ameter and cane yield of sugarcane, the soil microbial quantities and enzyme activi- ties were determined by using sugarcane as a material treated with different Cd concentrations (0, 25, 50, 100, 250 and 500 mg/kg) under potted conditions. IRe- suit] The results showed that the plant height, stem diameter and the yield of sug- arcane decreased with the increase of Cd concentration in the soil, and the higher the Cd concentration, the more obvious the inhibitory effect. The Cd contamination changed the enzyme activity, and the activities of urease and acid phosphatase sig- nificantly decreased with the increase of Cd concentration, especially when the Cd concentration reached 100 mg/kg. The sensitivity of the two soil enzymes to Cd ranked as urease〉acid phosphatase. Cd contamination also changed soil microbial quantities. Fungi, bacteria and actinomycetes significantly decreased at the Cd con- centration level of 100 mg/kg. There were significant and highly significant correla- tions between Cd contamination concentration and fungi, bacteria and actinomycetes, the activities of urease and acid phosphatase, plant height, stem diameter as well as cane yield. [Conclusion] Under the conditions of potted planted sugarcane, ex- ogenous Cd contamination affected the growth of sugarcane, the quantities of soil microorclanisms and soil enzyme activities to different degrees.展开更多
The effects of surface cracks caused by underground coal mining on microhabitat in the rhizosphere of Artem&ia ordosias were studied based on field investigation and sample analysis. The results show that the amounts...The effects of surface cracks caused by underground coal mining on microhabitat in the rhizosphere of Artem&ia ordosias were studied based on field investigation and sample analysis. The results show that the amounts of microorganisms, enzyme activity and water content in soil vary with the biomass ofA. ordosias, and that the account exhibits in an order of large A. ordosias〉medium A. ordosias〉small A. ordosias. Surface cracks apparently decrease microbial quantities and enzymatic ac- tivities, and change the composition and structure of microbial community in the rhizosphere of A. ordosias. Surface cracks reduce water content and electrical conductivity, enhance the R/S (ratio of root and soil) of water content, electrical conductivity and pH value, and raise the content of Na and Pb in rhizosphere soil ofA. ordosicas, It can be concluded that the disturbance of underground coal mining on the microhabitat in the rhizosphere ofA. ordosica is obvious in the early days.展开更多
基金The project was financially supported by the National key research and development plan“The technology of agricultural waste composting and the research of integrated equipment for composting”(2016YFD0800600).
摘要The purpose of this paper was to study the effects of different C/N ratios on the maturity and microbial quantity of com-posting with sesame meal and rice straw biochar.Rice straw was calcined into biochar as raw materials composting with sesame meal for 30 days,referring to Chenfu Agricultural Book in Sōuthern Song Dynasty(1127-1279 A.D.).Sesame meal was used to adjust the C/N ratio of compost,and three treatments were designed in the experiment,which were C/N ratios of 15,20 and 30,respectively.The results showed that C/N ratio of 20 was beneficial for promoting the temperature rise,the removal of water,the degradation of organic carbon,and the decrease of microbial quantity.The C/N ratio of 20 was beneficial to the compost maturity(T value was 0.47,final GI was 99.67%).The results of Pearson correlation analysis showed that C/N ratio was positively correlated with moisture content,total organic carbon and negatively correlated with germination index,indicating that high C/N ratio was beneficial to water removal and total organic carbon degradation.Therefore,we suggest that the suitable C/N ratio of rice straw biochar and sesame meal is 20.At the same time,we have proved that the composting method in Southern Song Dynasty is feasible,which is of great significance to understand the development of composting in China.
基金This study was funded by Natural Science Foundation of China(D011004):Ecological Restoration and Its Environmental Effects.
摘要Mechanical construction will put influence on the biological characters of reclaimed soils,as well as the soil quality.In order to explore the changing rule of soil microbial quantity and respiratory capacity under different construction machineries and rolling times,and find the optimal processing conditions,an experiment was set up and a simulation experimental area was chosen,in which we simulated the main types of reclamation in coal mine area.After 2 years’natural aging,we collected surface soil samples(0-20 cm)that can be used for experimental analysis.The result shows that changing rules of soil biological factors are different with different construction machineries,and soil properties are closest to the normal soil when adopting the combination of“crawler dozer×5 compaction times”and“dump truck×3 compaction times”,which shows that the soil quality is better under this condition.
基金supported by the National Hi-Tech Research and Development Program(863)of China(No.2007AA06Z329)the Science and Technology Project of Zhejiang Province(No.2007C23036)+1 种基金the Internation-al Cooperation Project in Science and Technology of Zhejiang Province(No.2008C14038)the National Science Foundation of Zhejiang Provience,China(No.19808)
摘要To investig ate the in fluen ce of bensulfuron-methyl(BSM)on culturable microbial quantities and un cul turable microbial community structures,conventional and molecularbiolo gical meth ods were cmplo yed in five BSM treated soils with threc replications,respectively.The results obtained with traditional culture-dependent methods sh owed that a low-level of BSM had slight and trans ient cffects on culturable microorganisms;nevertheless,high concentration of BSM resulted in a dramatic decrease in bacterial colony forming units(cfus).The result obtained using denaturing gradient gel electrophoresis(DGGE)revealed that more than 17 bands were observed in Iow BSM contaminated soil samples and only IO bands were detected in samples with high BSM contamination.In other word s,the diversity of soil community structure is related to the concentration of BSM.Cluster analysis sho wed that the community structure und cr low lev el of con tamination was more similar to that of the control,while heavy contamin ated amendments were far aw ay from the above group.In a sen se,the cooperation of the tradition al method and the molecular biological method is more powerful to study the soil microbial information in contamin ated ccos ys tem.
摘要[Objective] To provide a reference for exploring the relationship between Cd contamination and sugarcane growth and between Cd contamination and micro- bial properties of soil, the effects of adding different concentrations of exogenous cadmium (Cd) on the growth of sugarcane, the quantities of soil microorganisms and the activity of soil enzymes were studied. [Method] The plant height, stem di- ameter and cane yield of sugarcane, the soil microbial quantities and enzyme activi- ties were determined by using sugarcane as a material treated with different Cd concentrations (0, 25, 50, 100, 250 and 500 mg/kg) under potted conditions. IRe- suit] The results showed that the plant height, stem diameter and the yield of sug- arcane decreased with the increase of Cd concentration in the soil, and the higher the Cd concentration, the more obvious the inhibitory effect. The Cd contamination changed the enzyme activity, and the activities of urease and acid phosphatase sig- nificantly decreased with the increase of Cd concentration, especially when the Cd concentration reached 100 mg/kg. The sensitivity of the two soil enzymes to Cd ranked as urease〉acid phosphatase. Cd contamination also changed soil microbial quantities. Fungi, bacteria and actinomycetes significantly decreased at the Cd con- centration level of 100 mg/kg. There were significant and highly significant correla- tions between Cd contamination concentration and fungi, bacteria and actinomycetes, the activities of urease and acid phosphatase, plant height, stem diameter as well as cane yield. [Conclusion] Under the conditions of potted planted sugarcane, ex- ogenous Cd contamination affected the growth of sugarcane, the quantities of soil microorclanisms and soil enzyme activities to different degrees.
摘要The effects of surface cracks caused by underground coal mining on microhabitat in the rhizosphere of Artem&ia ordosias were studied based on field investigation and sample analysis. The results show that the amounts of microorganisms, enzyme activity and water content in soil vary with the biomass ofA. ordosias, and that the account exhibits in an order of large A. ordosias〉medium A. ordosias〉small A. ordosias. Surface cracks apparently decrease microbial quantities and enzymatic ac- tivities, and change the composition and structure of microbial community in the rhizosphere of A. ordosias. Surface cracks reduce water content and electrical conductivity, enhance the R/S (ratio of root and soil) of water content, electrical conductivity and pH value, and raise the content of Na and Pb in rhizosphere soil ofA. ordosicas, It can be concluded that the disturbance of underground coal mining on the microhabitat in the rhizosphere ofA. ordosica is obvious in the early days.