The aim is to select a universal DNA barcode for identifying all poisonous medicinal plants in Chinese pharmacopoeia and their poisonous related species or adulterants. We chose 4 commonly used regions as candidate DN...The aim is to select a universal DNA barcode for identifying all poisonous medicinal plants in Chinese pharmacopoeia and their poisonous related species or adulterants. We chose 4 commonly used regions as candidate DNA barcodes(ITS2, psb A-trn H,matK and rbc L) and compared their identification efficiency in 106 species from 27 families and 65 genera totally. Data analysis was performed including the information of sequence alignment, inter/intra-specific genetic distance and data distribution, identification efficiency and the situation of Neighbor-Joining(NJ) phylogenetic trees. We found ITS2 sequence region had high variation, stable genetic distance and identification efficiency relatively. The topological structure of NJ phylogenetic tree showed monophyletic. Our findings show that ITS2 can be applied as a universal barcode for identifying poisonous medicinal plants in Chinese pharmacopoeia and their poisonous related species or adulterants.展开更多
Objective Poisonous plants are a deadly threat to public health in China. The traditional clinical diagnosis of the toxic plants is inefficient, fallible, and dependent upon experts. In this study, we tested the perfo...Objective Poisonous plants are a deadly threat to public health in China. The traditional clinical diagnosis of the toxic plants is inefficient, fallible, and dependent upon experts. In this study, we tested the performance of DNA barcodes for identification of the most threatening poisonous plants in China. Methods Seventy-four accessions of 27 toxic plant species in 22 genera and 17 families were sampled and three DNA barcodes (motK, rbcL, and ITS) were amplified, sequenced and tested. Three methods, Blast, pairwise global alignment (PWG) distance, and Tree-Building were tested for discrimination power. Results The primer universality of all the three markers was high. Except in the case of ITS for Hemerocollis minor, the three barcodes were successfully generated from all the selected species. Among the three methods applied, Blast showed the lowest discrimination rate, whereas PWG Distance and Tree-Building methods were equally effective. The ITS barcode showed highest discrimination rates using the PWG Distance and Tree-Building methods. When the barcodes were combined, discrimination rates were increased for the Blast method.展开更多
Western grassland is the main source of living and means of production of western inhabitants. For many years, desertification and poisonous-weeds growth in grassland were resulted from over-grazing, over-reclaiming, ...Western grassland is the main source of living and means of production of western inhabitants. For many years, desertification and poisonous-weeds growth in grassland were resulted from over-grazing, over-reclaiming, over-spading and population growth. Western natural ecological environment is destroyed severly. Meanwhile, it has restricted the sustainable development of animal husbandry. The fast spreading poisonous-weeds, which caused grassland ecology unbalance, is one of the considerable bioecology problems and an important index of grassland degeneration. Based on analysis and induction of previous data, this article introduced the situation of poisonous-weeds disaster of western grassland in recent decades, category and distribution of poisonous-weeds, integrated control and reasonable utilization.展开更多
[Objective] Insecticidal activities of alkaloids extracted from 12 poisonous plants in Changbai Mountain were studied in the paper,to provide the basis for development and utilization of poisonous plants in Changbai M...[Objective] Insecticidal activities of alkaloids extracted from 12 poisonous plants in Changbai Mountain were studied in the paper,to provide the basis for development and utilization of poisonous plants in Changbai Mountain.[Method] Leaf disc method was used to study the effects of alkaloids extracted from 12 poisonous plants on the growth and development of 3-5 instars of cabbage worm (Pieris rapae) and their antifeedant activities.[Result] The effects of alkaloids extracted from different poisonous plants on the growth and development of cabbage worm were also different.Five treatments of alkaloids extracted from Sophora flavescens,Datura stramonium L.,Arisaema amurense Maxim.,Veratum dahuricum Loes.and Tripterygium regelii Spragne et Takeda made the weight of test insects gradually decrease and finally die;the alkaloid treatments of Pueraria lobata (Willd.) Ohwi,Aconitum kusnezoffii Reichb.,Cimicifuga dahurica (Turcz.) Maxim.and Corydalis ambigua Schleeht.could make cabbage worm pupate ahead of time,but the pupa state was deformed;the antifeedant rates of cabbage worm after the treatments of P.lobata,A.kusnezoffii,C.dahurica,A.amurense,V.dahuricum and C.ambigua for 48 h were all over 90%,and the antifeedant rates of C.dahurica and C.ambigua were the highest as 100%.[Conclusion]The reports on the insecticidal activities of five poisonous plants including P.lobata,A.kusnezoffii,C.dahurica,C.ambigua and A.amurense are rare,and they have important values on the development of botanical pesticides.展开更多
The article presents the composition and current state of poisonous plants distributed on the Ustyurt plateau. Ustyurt is one of the largest deserts in Central Asia, differing from other deserts of the world in geogra...The article presents the composition and current state of poisonous plants distributed on the Ustyurt plateau. Ustyurt is one of the largest deserts in Central Asia, differing from other deserts of the world in geographical location, relief, flora and fauna and other features. The territory of Ustyurt occupies 21.3 million hectares, of which the Karakalpak part accounts for 7.2 million hectares. Poisonous alkaloid-bearing plants of the Karakalpak part of the Ustyurt plateau Anabasis aphylla, Atriplex flabellum, Kalidium capsicum, Salsola arbusculaeformis, Salsola foliosa, Aellenia subaphylla, Anabasis brachiata, Rheum tataricum, Capparis spinosa, Glycyrrhiza aspera. Alkaloid-bearing plants that are not eaten or poorly eaten by cattle Ephedra distachya, Delphinium songaricum, Anabasis salsa, alkaloid-bearing plants eaten by animals without harm Carex physodes, Eremopyrum orientale, Agrophyllum repens, Astragalus amodendron, Astragalus villosissimus, species of the genera Calligonum, Salsola. Poisonous plants of the Karakalpak part of the Ustyurt plateau. An analysis of the pasture flora of the Karakalpak part of the Ustyurt plateau shows that 62 species of wild poisonous plants belonging to 49 genera and 19 families grow here. Although these plants are considered poisonous, but in modern pharmaceutics and medicine, they are used as medicinal.展开更多
The field control effects of different concentrations of indoxacarb baits,fipronil baits and chlorfenapyr baits on Solenopsis invicta was evaluated. The results showed that at 21 post administration,the control effect...The field control effects of different concentrations of indoxacarb baits,fipronil baits and chlorfenapyr baits on Solenopsis invicta was evaluated. The results showed that at 21 post administration,the control effects of 0. 04% indoxacarb on nests and worker ants reached 100%; the control effect of 0. 02% and0. 06% indoxacarb on active nests and worker ants were 87. 50% and 100%,respectively; 0. 000 25% and 0. 000 5% fipronil and 0. 05% chlorfenapyr also had significant control effects on active nests and worker ants. At 30 d post administration,the decline rates of ant colony grade treated by 0. 04% and 0. 06% indoxacarb,0. 000 25% fipronil and 0. 05% chlorfenapyr were 100%,100%,91. 44% and 98. 11%,respectively,and the comprehensive control effect reached 97. 18%.展开更多
This work utilizes a statistical approach of Principal Component Ana-lysis(PCA)towards the detection of Methane(CH4)-Carbon Monoxide(CO)Poi-soning occurring in coal mines,forestfires,drainage systems etc.where the ...This work utilizes a statistical approach of Principal Component Ana-lysis(PCA)towards the detection of Methane(CH4)-Carbon Monoxide(CO)Poi-soning occurring in coal mines,forestfires,drainage systems etc.where the CH4 and CO emissions are very high in closed buildings or confined spaces during oxi-dation processes.Both methane and carbon monoxide are highly toxic,colorless and odorless gases.Both of the gases have their own toxic levels to be detected.But during their combined presence,the toxicity of the either one goes unidentified may be due to their low levels which may lead to an explosion.By using PCA,the correlation of CO and CH4 data is carried out and by identifying the areas of high correlation(along the principal component axis)the explosion suppression action can be triggered earlier thus avoiding adverse effects of massive explosions.Wire-less Sensor Network is deployed and simulations are carried with heterogeneous sensors(Carbon Monoxide and Methane sensors)in NS-2 Mannasim framework.The rise in the value of CO even when CH4 is below the toxic level may become hazardous to the people around.Thus our proposed methodology will detect the combined presence of both the gases(CH4 and CO)and provide an early warning in order to avoid any human losses or toxic effects.展开更多
AIM To report our experience of acute kidney injury(AKI) developed after exposure to poisonous substance.METHODS Retrospective study where data was collected from case records of patients coming to this institute duri...AIM To report our experience of acute kidney injury(AKI) developed after exposure to poisonous substance.METHODS Retrospective study where data was collected from case records of patients coming to this institute during January 1990 to May 2016.This institution is a tertiary care center for renal care in the metropolitan city of Karachi,Pakistan.History of ingested substance,symptoms on presentation,basic laboratory tests on arrival,mode of treatment and outcome were recorded from all patients and are presented here.Patients developing AKI after snake envenomation or scorpion stings are not included in this study.RESULTS During studied period 184 cases of AKI developing after poisoning were seen at our institution.The largest group was from paraphenyline diamine poisoning comprising 135 patients,followed by methanol in 8,organophosphorus compounds in 5,paraquat in 5,copper sulphate in 5,tartaric acid in 4,phenobarbitone in 3 and benzodiazipines,datura,rat killer,fish gall bladder,arsenic,boiler water,ammonium dichromate,acetic acid and herbs with lesser frequency.In 8 patients multiple substances were ingested in combination.Renal replacement therapy was required in 96% of patients.Complete recovery was seen in 72.28% patients,20% died during acute phase of illness.CONCLUSION It is important to report poisonous substances causing vital organ failure to increase awareness among general population as well as health care providers.展开更多
Maqianzi(马钱子Strychnos)is also called“Fanmubie(番木鳖),"“Kushi(苦实)”and“QianJi drug(牵机药).”The alias“Fanmubie”shows its origin and its characteristics.The name of“Kushi”implies its taste and flavor....Maqianzi(马钱子Strychnos)is also called“Fanmubie(番木鳖),"“Kushi(苦实)”and“QianJi drug(牵机药).”The alias“Fanmubie”shows its origin and its characteristics.The name of“Kushi”implies its taste and flavor.“Qianji Drug vividly shows the clinical manifestations of the poisoning of Maqianzi.The name of“Maqianzi”is the comprehensive display of its characteristic,toxicity,etc.,Maqianzi is famous for its poison,which is often used in the treatment of various intractable diseases.It shows the poison Culture in the Western Regions,meanwhile it also displays the unique charm of Traditional herbs in transforming poison into treasure.展开更多
The term“worm medicines”(Chong Yao)in Traditional Chinese Medicine encompasses a diverse category of small animal-derived therapeutics,including arthropods,amphibians,and reptiles,rather than strictly vermiform orga...The term“worm medicines”(Chong Yao)in Traditional Chinese Medicine encompasses a diverse category of small animal-derived therapeutics,including arthropods,amphibians,and reptiles,rather than strictly vermiform organisms.Many of these animals,such as scorpions,centipedes,toads,and horseflies,are toxic,and have been employed in clinical practice for centuries,despite limited understanding of their active compounds and underlying modes of action.Among the bioactive constituents extracted from these taxa,peptides represent a particularly promising class,with over 2000 identified to date.These peptides are primarily secreted from specialized exocrine glands,including venom,salivary,and cutaneous glands,and are characterized by high structural diversity and target specificity.Their potent modulatory effects on the nervous,cardiovascular,and immune systems make them excellent candidates for drug discovery and development.This review examines peptide-based compounds derived from the exocrine secretions of venomous and poisonous taxa historically employed in traditional Chinese“worm”medicines.Emphasis is placed on their biological origins,structural features,and pharmacological activities,highlighting their significant potential as a rich and largely untapped resource for modern therapeutic discovery.展开更多
AB2-type Ti-based hydrogen storage alloys(HSAs)are promising for industrial hydrogen feeding systems due to their moderate operating conditions and high hydrogen storage capacity.However,their practical application...AB2-type Ti-based hydrogen storage alloys(HSAs)are promising for industrial hydrogen feeding systems due to their moderate operating conditions and high hydrogen storage capacity.However,their practical application is hindered by unavoidable impurity gases in hydrogen feedstocks,which significantly impair the performance of HSAs.Furthermore,the absence of clear evaluation criteria for poisoning behaviors and mechanisms hinders efforts to develop effective mitigation strategies.To address this gap,we used calculated surface interaction energy changes(ΔE)and experimental investigations to classify and rank the poisoning potential of impurity gases on a C14 Laves-phase Ti0.86Zr0.15Mn1.5Cr0.07(VFe)0.43 alloy.Impurity gases were classified into two types of weak-adsorption and strong-adsorption impurity gases by comparing theirΔE with that of H2(ΔE_(H2)=-1.6001 eV).AsΔE>ΔE_(H2) ,weak-adsorption impurity gases(Ar,He,CH4,and N2)induce poisoning by forming enriched blocking layers that impede H2 diffusion.This blocking effect can be alleviated under gas flow conditions.AsΔE<ΔE_(H2),strong adsorption gases are further divided into two types based on their reactivity with the alloy.Non-reactive strong-adsorption impurity gases(CO and CO2 )preferentially occupy surface active sites,blocking H2 adsorption and dissociation.In contrast,reactive strong-adsorption impurity gases(such as O2)form dense passivation layers that completely prevent hydrogen ingress.Accordingly,surface modification offers an effective approach to mitigate gas-induced poisoning by altering the interaction mechanism.This study establishes the parameter-based criteria for classifying impurity gas poisoning mechanisms in AB2-type Ti-based HSAs.It provides fundamental insights for guiding the design of poisoning-resistant materials and the development of mitigation strategies.展开更多
The performance of proton-exchange-membrane fuel cell(PEMFC)is known to be highly restrained by the nano-micro-scale ionomer-catalyst interface structure.The commonly used ionomers in the catalyst layer are functional...The performance of proton-exchange-membrane fuel cell(PEMFC)is known to be highly restrained by the nano-micro-scale ionomer-catalyst interface structure.The commonly used ionomers in the catalyst layer are functionalized by forming a nanoscale network with platinum-group-metal(PGM)based catalyst.The imbalanced binding strengths of sulfonate groups to the PGM surface compared to the carbon support surface lead to the favorable occupation of active sites for oxygen reduction reaction(ORR)and the declining performances in membrane electrode assembly(MEA).In this work,by employing a dual strategy on PGM and carbon support,a catalyst/support design is proposed to significantly reduce the variance in adsorption energies of sulfonate to the PGM and carbon surface.The balanced adsorption of sulfonate groups enables a more uniform distribution of ionomer on the catalyst surface with enhanced catalytic activity.The PtCo/CNH-N catalyst not only achieves a power density far exceeding that of its counterpart in MEA but also exhibits high stability against ionomer poisoning throughout potential cycling.Combining experimental evidence and theoretical calculation,this work corroborates the essential role of balanced binding strengths of sulfonate groups to PGM and carbon surface,and provides insight into the ionomer poisoning issue towards the design of a highly stable catalyst for PEMFC.展开更多
The high sensitivity of platinum(Pt)-based catalysts to CO during the hydrogen oxidation reaction(HOR)at the anode is one of the key issues for the long-term stable development of proton exchange membrane fuel cells(P...The high sensitivity of platinum(Pt)-based catalysts to CO during the hydrogen oxidation reaction(HOR)at the anode is one of the key issues for the long-term stable development of proton exchange membrane fuel cells(PEMFCs).Modulating the electronic structure of Pt is considered an effective approach to enhancing HOR activity and improving CO tolerance.Herein,we utilized the synergistic effect between the transition metal interstitial compounds(TMICs)of VN and Pt to develop a Pt-VN heterojunction-loaded carbon nanofiber catalyst(Pt-VN/NCNF)for CO tolerance in HOR.The introduction of VN causes electronic orbitals rearrangement of Pt,thereby optimizing the adsorption of H on the Pt surface.Meanwhile,the overlap of the d-band of the electron-deficient Pt with the 1πand 5σbonding orbitals of CO was significantly reduced,which suppresses the strong CO adsorption on Pt surfaces and leave more active sites for H2adsorption and oxidation.As a result,Pt-VN/NCNF exhibits a mass activity of 1.26 m A/μgPt,41 times higher than that of commercial Pt/C.Encouragingly,Pt-VN/NCNF maintains 96.7%of its original activity even in the presence of 1000 ppm CO.As anticipated,Pt-VN/NCNF-based PEMFCs demonstrate superior CO tolerance to Pt/C in H2/CO mixtures with CO concentrations ranging from 10 ppm to 1000 ppm.展开更多
Low-pressure ammonia synthesis technology holds revolutionary significance in ensuring food security,promoting energy transition,and addressing climate change.However,the hydrogen poisoning of catalysts has long restr...Low-pressure ammonia synthesis technology holds revolutionary significance in ensuring food security,promoting energy transition,and addressing climate change.However,the hydrogen poisoning of catalysts has long restricted the development of low-pressure ammonia synthesis processes.Focusing on the regulation of support surface properties,this study successfully prepared a Ru/Al2O3-La-C catalyst.By regulating the transfer and desorption pathways of hydrogen on the catalyst surface,the hydrogen poisoning phenomenon under low-pressure conditions was effectively alleviated.Under the reaction conditions of 400℃and 3 MPa,the ammonia synthesis yield of the Ru/Al2O3-La-C catalyst reached 4.74 mmol NH3·gcat-1·h-1,which was 10 times higher than that of the unmodified Ru/Al2O3catalyst.Meanwhile,the reaction order of hydrogen changed from−0.43 to 0.58,and the reaction order of nitrogen changed from 0.98 to 0.44,further confirming that the hydrogen poisoning issue of the catalyst was significantly improved and the activation of nitrogen was promoted.Characterization results from H2-TPD and H-D exchange experiments showed that La doping constructed La-O sites,which facilitated the transfer of active hydrogen from the active component Ru to the support.In contrast,the introduction of carbon regulated the recombination and desorption mode of hydrogen on the surface of metallic Ru,prompting hydrogen to desorb in the form of H2instead of forming H2O.This study provides a new insight for the design and development of catalysts for low-pressure ammonia synthesis.展开更多
Staphylococcus aureus(S.aureus)is the third most common pathogen causing 10.6%of bacterial foodborne illnesses in China in 2021[1].Heat-stable Staphylococcal Enterotoxins(SEs)produced by S.aureus are the main contribu...Staphylococcus aureus(S.aureus)is the third most common pathogen causing 10.6%of bacterial foodborne illnesses in China in 2021[1].Heat-stable Staphylococcal Enterotoxins(SEs)produced by S.aureus are the main contributors to staphylococcal food poisoning(SFP),causing vomiting,diarrhea,abdominal pain,headache,muscle cramps,and other acute gastroenteritis symptoms.More than 25 SEs and staphylococcal enterotoxin-like toxins(SE/s)have been described and which together comprise a superfamily of pyrogenic toxin superantigens(SAgs)[2].展开更多
The performance of deep recommendation models degrades significantly under data poisoning attacks.While adversarial training methods such as Vulnerability-Aware Training(VAT)enhance robustness by injecting perturbatio...The performance of deep recommendation models degrades significantly under data poisoning attacks.While adversarial training methods such as Vulnerability-Aware Training(VAT)enhance robustness by injecting perturbations into embeddings,they remain limited by coarse-grained noise and a static defense strategy,leaving models susceptible to adaptive attacks.This study proposes a novel framework,Self-Purification Data Sanitization(SPD),which integrates vulnerability-aware adversarial training with dynamic label correction.Specifically,SPD first identifies high-risk users through a fragility scoring mechanism,then applies self-purification by replacing suspicious interactions with model-predicted high-confidence labels during training.This closed-loop process continuously sanitizes the training data and breaks the protection ceiling of conventional adversarial training.Experiments demonstrate that SPD significantly improves the robustness of both Matrix Factorization(MF)and LightGCN models against various poisoning attacks.We show that SPD effectively suppresses malicious gradient propagation and maintains recommendation accuracy.Evaluations on Gowalla and Yelp2018 confirmthat SPD-trainedmodels withstandmultiple attack strategies—including Random,Bandwagon,DP,and Rev attacks—while preserving performance.展开更多
BACKGROUND:This study is to evaluate clearance eff ects of hemoperfusion(HP),continuous renal replacement therapy(CRRT),and plasma exchange(PE)for chlorfenapyr and its metabolite tralopyril in patients with acute pois...BACKGROUND:This study is to evaluate clearance eff ects of hemoperfusion(HP),continuous renal replacement therapy(CRRT),and plasma exchange(PE)for chlorfenapyr and its metabolite tralopyril in patients with acute poisoning.METHODS:This retrospective study included 18 patients with acute oral chlorfenapyr poisoning treated at our department between January 2022 and January 2024.All patients received conventional therapies combined with blood purification,including HP,CRRT,and PE.HP was performed three sessions within the fi rst 24 h,followed by CRRT and PE.Serial blood samples were collected to measure plasma concentrations of chlorfenapyr and tralopyril using gas chromatography/liquid chromatography-mass spectrometry(GC/LC-MS).The toxin-clearance eff ects were assessed using a linear mixed-eff ects(LME)model.RESULTS:The hourly decline rate of the plasma chlorfenapyr concentration(median[IQR])was 8.83%(1.79%)for HP,4.12%(1.26%)for CRRT,and 6.85%(1.44%)for PE.LME analysis showed higher decline rate in the plasma concentration with HP(β=5.00;P<0.001)and PE(β=2.15;P=0.003)compared to CRRT.For tralopyril,the hourly decline rates were 3.04%(0.62%)for HP,1.82%(0.48%)for CRRT,and 3.01%(0.37%)for PE.LME analysis showed that the clearance effects of HP(β=0.027;P<0.001)and PE(β=0.022;P=0.001)were superior to CRRT.Pre-treatment toxin levels and the interval from hospital admission to blood purifi cation showed no signifi cant interaction with clearance outcomes.CONCLUSION:In our study,HP was associated with a higher decline rate in plasma chlorfenapyr concentration compared to CRRT and PE,supporting HP as a preferred early intervention.However,all three methods showed limited effi cacy in reducing tralopyril levels.Further research into the toxicokinetics and mechanisms of chlorfenapyr is warranted to optimize purifi cation strategies.展开更多
In some cases, poisonous contaminants may be released from bottom sludge inopen channels. The equation of advection and diffusion for the related problem was analyzed in thispaper. The conditions for the definite solu...In some cases, poisonous contaminants may be released from bottom sludge inopen channels. The equation of advection and diffusion for the related problem was analyzed in thispaper. The conditions for the definite solution to the equation were given. The analytic solution ofpoisonous gas concentration distribution was worked out. The reasonableness of this solution wasdiscussed. The result is also of significance for other similar problems.展开更多
基金supported by National Standardization Research Project(Nos.ZYBZH-Y-TJ-43,ZYBZH-C-JS-37,ZYBZH-C-JS-29,and ZYBZH-Y-JIN-34)Independent project of Institute of Chinese Materia Medica,Academy of Chinese Medical Sciences(No.ZXKT17021)+1 种基金Key Research Project of China Academy of Chinese Medical Sciences of the 13th Five-Year Plan(No.ZZ10-007)National key research and development plan(No.2017YFB1002303)
摘要The aim is to select a universal DNA barcode for identifying all poisonous medicinal plants in Chinese pharmacopoeia and their poisonous related species or adulterants. We chose 4 commonly used regions as candidate DNA barcodes(ITS2, psb A-trn H,matK and rbc L) and compared their identification efficiency in 106 species from 27 families and 65 genera totally. Data analysis was performed including the information of sequence alignment, inter/intra-specific genetic distance and data distribution, identification efficiency and the situation of Neighbor-Joining(NJ) phylogenetic trees. We found ITS2 sequence region had high variation, stable genetic distance and identification efficiency relatively. The topological structure of NJ phylogenetic tree showed monophyletic. Our findings show that ITS2 can be applied as a universal barcode for identifying poisonous medicinal plants in Chinese pharmacopoeia and their poisonous related species or adulterants.
基金supported by the Fundamental Research Funds for the Central Universities(grant no.TD2012-04)the Scientific Research Foundation of the State Human Resource Ministry and the Education Ministry for Returned Chinese Scholars(to L.Xie)+2 种基金the State Key Laboratory Program from the State Key Laboratory of Systematic and Evolutionary Botany(grant no.LSEB2011-07)the Main Direction Program of Knowledge Innovation of the Chinese Academy of Sciences(grant no.KSCX2-EW-Z-1)the National Natural Science Foundation of China(grant nos.31170201,81072317,31110103911)
摘要Objective Poisonous plants are a deadly threat to public health in China. The traditional clinical diagnosis of the toxic plants is inefficient, fallible, and dependent upon experts. In this study, we tested the performance of DNA barcodes for identification of the most threatening poisonous plants in China. Methods Seventy-four accessions of 27 toxic plant species in 22 genera and 17 families were sampled and three DNA barcodes (motK, rbcL, and ITS) were amplified, sequenced and tested. Three methods, Blast, pairwise global alignment (PWG) distance, and Tree-Building were tested for discrimination power. Results The primer universality of all the three markers was high. Except in the case of ITS for Hemerocollis minor, the three barcodes were successfully generated from all the selected species. Among the three methods applied, Blast showed the lowest discrimination rate, whereas PWG Distance and Tree-Building methods were equally effective. The ITS barcode showed highest discrimination rates using the PWG Distance and Tree-Building methods. When the barcodes were combined, discrimination rates were increased for the Blast method.
基金funded by National Nature Science Foundation of China(31072175)Tibet Project Supported by Ministry of Agriculture of the People's Republic of China(20071010)
摘要Western grassland is the main source of living and means of production of western inhabitants. For many years, desertification and poisonous-weeds growth in grassland were resulted from over-grazing, over-reclaiming, over-spading and population growth. Western natural ecological environment is destroyed severly. Meanwhile, it has restricted the sustainable development of animal husbandry. The fast spreading poisonous-weeds, which caused grassland ecology unbalance, is one of the considerable bioecology problems and an important index of grassland degeneration. Based on analysis and induction of previous data, this article introduced the situation of poisonous-weeds disaster of western grassland in recent decades, category and distribution of poisonous-weeds, integrated control and reasonable utilization.
基金Supported by School Fund in Yanbian University (201015)~~
摘要[Objective] Insecticidal activities of alkaloids extracted from 12 poisonous plants in Changbai Mountain were studied in the paper,to provide the basis for development and utilization of poisonous plants in Changbai Mountain.[Method] Leaf disc method was used to study the effects of alkaloids extracted from 12 poisonous plants on the growth and development of 3-5 instars of cabbage worm (Pieris rapae) and their antifeedant activities.[Result] The effects of alkaloids extracted from different poisonous plants on the growth and development of cabbage worm were also different.Five treatments of alkaloids extracted from Sophora flavescens,Datura stramonium L.,Arisaema amurense Maxim.,Veratum dahuricum Loes.and Tripterygium regelii Spragne et Takeda made the weight of test insects gradually decrease and finally die;the alkaloid treatments of Pueraria lobata (Willd.) Ohwi,Aconitum kusnezoffii Reichb.,Cimicifuga dahurica (Turcz.) Maxim.and Corydalis ambigua Schleeht.could make cabbage worm pupate ahead of time,but the pupa state was deformed;the antifeedant rates of cabbage worm after the treatments of P.lobata,A.kusnezoffii,C.dahurica,A.amurense,V.dahuricum and C.ambigua for 48 h were all over 90%,and the antifeedant rates of C.dahurica and C.ambigua were the highest as 100%.[Conclusion]The reports on the insecticidal activities of five poisonous plants including P.lobata,A.kusnezoffii,C.dahurica,C.ambigua and A.amurense are rare,and they have important values on the development of botanical pesticides.
摘要The article presents the composition and current state of poisonous plants distributed on the Ustyurt plateau. Ustyurt is one of the largest deserts in Central Asia, differing from other deserts of the world in geographical location, relief, flora and fauna and other features. The territory of Ustyurt occupies 21.3 million hectares, of which the Karakalpak part accounts for 7.2 million hectares. Poisonous alkaloid-bearing plants of the Karakalpak part of the Ustyurt plateau Anabasis aphylla, Atriplex flabellum, Kalidium capsicum, Salsola arbusculaeformis, Salsola foliosa, Aellenia subaphylla, Anabasis brachiata, Rheum tataricum, Capparis spinosa, Glycyrrhiza aspera. Alkaloid-bearing plants that are not eaten or poorly eaten by cattle Ephedra distachya, Delphinium songaricum, Anabasis salsa, alkaloid-bearing plants eaten by animals without harm Carex physodes, Eremopyrum orientale, Agrophyllum repens, Astragalus amodendron, Astragalus villosissimus, species of the genera Calligonum, Salsola. Poisonous plants of the Karakalpak part of the Ustyurt plateau. An analysis of the pasture flora of the Karakalpak part of the Ustyurt plateau shows that 62 species of wild poisonous plants belonging to 49 genera and 19 families grow here. Although these plants are considered poisonous, but in modern pharmaceutics and medicine, they are used as medicinal.
基金Supported by Project of Liuzhou Science and Technology Bureau"Investigation and Prevention Countermeasures of Fire Ants in Liuzhou City"(2013J020101)
摘要The field control effects of different concentrations of indoxacarb baits,fipronil baits and chlorfenapyr baits on Solenopsis invicta was evaluated. The results showed that at 21 post administration,the control effects of 0. 04% indoxacarb on nests and worker ants reached 100%; the control effect of 0. 02% and0. 06% indoxacarb on active nests and worker ants were 87. 50% and 100%,respectively; 0. 000 25% and 0. 000 5% fipronil and 0. 05% chlorfenapyr also had significant control effects on active nests and worker ants. At 30 d post administration,the decline rates of ant colony grade treated by 0. 04% and 0. 06% indoxacarb,0. 000 25% fipronil and 0. 05% chlorfenapyr were 100%,100%,91. 44% and 98. 11%,respectively,and the comprehensive control effect reached 97. 18%.
摘要This work utilizes a statistical approach of Principal Component Ana-lysis(PCA)towards the detection of Methane(CH4)-Carbon Monoxide(CO)Poi-soning occurring in coal mines,forestfires,drainage systems etc.where the CH4 and CO emissions are very high in closed buildings or confined spaces during oxi-dation processes.Both methane and carbon monoxide are highly toxic,colorless and odorless gases.Both of the gases have their own toxic levels to be detected.But during their combined presence,the toxicity of the either one goes unidentified may be due to their low levels which may lead to an explosion.By using PCA,the correlation of CO and CH4 data is carried out and by identifying the areas of high correlation(along the principal component axis)the explosion suppression action can be triggered earlier thus avoiding adverse effects of massive explosions.Wire-less Sensor Network is deployed and simulations are carried with heterogeneous sensors(Carbon Monoxide and Methane sensors)in NS-2 Mannasim framework.The rise in the value of CO even when CH4 is below the toxic level may become hazardous to the people around.Thus our proposed methodology will detect the combined presence of both the gases(CH4 and CO)and provide an early warning in order to avoid any human losses or toxic effects.
摘要AIM To report our experience of acute kidney injury(AKI) developed after exposure to poisonous substance.METHODS Retrospective study where data was collected from case records of patients coming to this institute during January 1990 to May 2016.This institution is a tertiary care center for renal care in the metropolitan city of Karachi,Pakistan.History of ingested substance,symptoms on presentation,basic laboratory tests on arrival,mode of treatment and outcome were recorded from all patients and are presented here.Patients developing AKI after snake envenomation or scorpion stings are not included in this study.RESULTS During studied period 184 cases of AKI developing after poisoning were seen at our institution.The largest group was from paraphenyline diamine poisoning comprising 135 patients,followed by methanol in 8,organophosphorus compounds in 5,paraquat in 5,copper sulphate in 5,tartaric acid in 4,phenobarbitone in 3 and benzodiazipines,datura,rat killer,fish gall bladder,arsenic,boiler water,ammonium dichromate,acetic acid and herbs with lesser frequency.In 8 patients multiple substances were ingested in combination.Renal replacement therapy was required in 96% of patients.Complete recovery was seen in 72.28% patients,20% died during acute phase of illness.CONCLUSION It is important to report poisonous substances causing vital organ failure to increase awareness among general population as well as health care providers.
摘要Maqianzi(马钱子Strychnos)is also called“Fanmubie(番木鳖),"“Kushi(苦实)”and“QianJi drug(牵机药).”The alias“Fanmubie”shows its origin and its characteristics.The name of“Kushi”implies its taste and flavor.“Qianji Drug vividly shows the clinical manifestations of the poisoning of Maqianzi.The name of“Maqianzi”is the comprehensive display of its characteristic,toxicity,etc.,Maqianzi is famous for its poison,which is often used in the treatment of various intractable diseases.It shows the poison Culture in the Western Regions,meanwhile it also displays the unique charm of Traditional herbs in transforming poison into treasure.
基金supported by the Key Research and Development Program of Guangzhou Science and Technology Plan Project(2024B03J0011)National Natural Science Foundation of China(32522015,32570586,32370538)+1 种基金National Key Research and Development Program of China(2023YFF1304900)Yunnan Province Grant(2021000097,202402AA310010,202403AC100010)。
摘要The term“worm medicines”(Chong Yao)in Traditional Chinese Medicine encompasses a diverse category of small animal-derived therapeutics,including arthropods,amphibians,and reptiles,rather than strictly vermiform organisms.Many of these animals,such as scorpions,centipedes,toads,and horseflies,are toxic,and have been employed in clinical practice for centuries,despite limited understanding of their active compounds and underlying modes of action.Among the bioactive constituents extracted from these taxa,peptides represent a particularly promising class,with over 2000 identified to date.These peptides are primarily secreted from specialized exocrine glands,including venom,salivary,and cutaneous glands,and are characterized by high structural diversity and target specificity.Their potent modulatory effects on the nervous,cardiovascular,and immune systems make them excellent candidates for drug discovery and development.This review examines peptide-based compounds derived from the exocrine secretions of venomous and poisonous taxa historically employed in traditional Chinese“worm”medicines.Emphasis is placed on their biological origins,structural features,and pharmacological activities,highlighting their significant potential as a rich and largely untapped resource for modern therapeutic discovery.
基金financially supported by the National Key Research and Development Program of China(2022YFB4004302)the National Natural Science Foundation of China(U24A2044)the Guangxi Science and Technology Major Project(No.AA24206007)。
摘要AB2-type Ti-based hydrogen storage alloys(HSAs)are promising for industrial hydrogen feeding systems due to their moderate operating conditions and high hydrogen storage capacity.However,their practical application is hindered by unavoidable impurity gases in hydrogen feedstocks,which significantly impair the performance of HSAs.Furthermore,the absence of clear evaluation criteria for poisoning behaviors and mechanisms hinders efforts to develop effective mitigation strategies.To address this gap,we used calculated surface interaction energy changes(ΔE)and experimental investigations to classify and rank the poisoning potential of impurity gases on a C14 Laves-phase Ti0.86Zr0.15Mn1.5Cr0.07(VFe)0.43 alloy.Impurity gases were classified into two types of weak-adsorption and strong-adsorption impurity gases by comparing theirΔE with that of H2(ΔE_(H2)=-1.6001 eV).AsΔE>ΔE_(H2) ,weak-adsorption impurity gases(Ar,He,CH4,and N2)induce poisoning by forming enriched blocking layers that impede H2 diffusion.This blocking effect can be alleviated under gas flow conditions.AsΔE<ΔE_(H2),strong adsorption gases are further divided into two types based on their reactivity with the alloy.Non-reactive strong-adsorption impurity gases(CO and CO2 )preferentially occupy surface active sites,blocking H2 adsorption and dissociation.In contrast,reactive strong-adsorption impurity gases(such as O2)form dense passivation layers that completely prevent hydrogen ingress.Accordingly,surface modification offers an effective approach to mitigate gas-induced poisoning by altering the interaction mechanism.This study establishes the parameter-based criteria for classifying impurity gas poisoning mechanisms in AB2-type Ti-based HSAs.It provides fundamental insights for guiding the design of poisoning-resistant materials and the development of mitigation strategies.
基金the financial support from the National Natural Science Foundation of China(No.52171199,No.52211530442,and No.22479011)。
摘要The performance of proton-exchange-membrane fuel cell(PEMFC)is known to be highly restrained by the nano-micro-scale ionomer-catalyst interface structure.The commonly used ionomers in the catalyst layer are functionalized by forming a nanoscale network with platinum-group-metal(PGM)based catalyst.The imbalanced binding strengths of sulfonate groups to the PGM surface compared to the carbon support surface lead to the favorable occupation of active sites for oxygen reduction reaction(ORR)and the declining performances in membrane electrode assembly(MEA).In this work,by employing a dual strategy on PGM and carbon support,a catalyst/support design is proposed to significantly reduce the variance in adsorption energies of sulfonate to the PGM and carbon surface.The balanced adsorption of sulfonate groups enables a more uniform distribution of ionomer on the catalyst surface with enhanced catalytic activity.The PtCo/CNH-N catalyst not only achieves a power density far exceeding that of its counterpart in MEA but also exhibits high stability against ionomer poisoning throughout potential cycling.Combining experimental evidence and theoretical calculation,this work corroborates the essential role of balanced binding strengths of sulfonate groups to PGM and carbon surface,and provides insight into the ionomer poisoning issue towards the design of a highly stable catalyst for PEMFC.
基金supported by the National Natural Science Foundation of China(Nos.22179034,22279030)the Natural Science Foundation of Heilongjiang Province(No.ZD2023B002)。
摘要The high sensitivity of platinum(Pt)-based catalysts to CO during the hydrogen oxidation reaction(HOR)at the anode is one of the key issues for the long-term stable development of proton exchange membrane fuel cells(PEMFCs).Modulating the electronic structure of Pt is considered an effective approach to enhancing HOR activity and improving CO tolerance.Herein,we utilized the synergistic effect between the transition metal interstitial compounds(TMICs)of VN and Pt to develop a Pt-VN heterojunction-loaded carbon nanofiber catalyst(Pt-VN/NCNF)for CO tolerance in HOR.The introduction of VN causes electronic orbitals rearrangement of Pt,thereby optimizing the adsorption of H on the Pt surface.Meanwhile,the overlap of the d-band of the electron-deficient Pt with the 1πand 5σbonding orbitals of CO was significantly reduced,which suppresses the strong CO adsorption on Pt surfaces and leave more active sites for H2adsorption and oxidation.As a result,Pt-VN/NCNF exhibits a mass activity of 1.26 m A/μgPt,41 times higher than that of commercial Pt/C.Encouragingly,Pt-VN/NCNF maintains 96.7%of its original activity even in the presence of 1000 ppm CO.As anticipated,Pt-VN/NCNF-based PEMFCs demonstrate superior CO tolerance to Pt/C in H2/CO mixtures with CO concentrations ranging from 10 ppm to 1000 ppm.
基金supported by the National Key R&D Program of China(Grant 2022YFA1506200)The National Natural Science Foundation of China(Grants U23B20164,22408361,22288102)Open Project Program of State Key Laboratory of Low-carbon Smart Coal-fired Power Generation and Ultra-clean Emission(Grant D2024FK143).
摘要Low-pressure ammonia synthesis technology holds revolutionary significance in ensuring food security,promoting energy transition,and addressing climate change.However,the hydrogen poisoning of catalysts has long restricted the development of low-pressure ammonia synthesis processes.Focusing on the regulation of support surface properties,this study successfully prepared a Ru/Al2O3-La-C catalyst.By regulating the transfer and desorption pathways of hydrogen on the catalyst surface,the hydrogen poisoning phenomenon under low-pressure conditions was effectively alleviated.Under the reaction conditions of 400℃and 3 MPa,the ammonia synthesis yield of the Ru/Al2O3-La-C catalyst reached 4.74 mmol NH3·gcat-1·h-1,which was 10 times higher than that of the unmodified Ru/Al2O3catalyst.Meanwhile,the reaction order of hydrogen changed from−0.43 to 0.58,and the reaction order of nitrogen changed from 0.98 to 0.44,further confirming that the hydrogen poisoning issue of the catalyst was significantly improved and the activation of nitrogen was promoted.Characterization results from H2-TPD and H-D exchange experiments showed that La doping constructed La-O sites,which facilitated the transfer of active hydrogen from the active component Ru to the support.In contrast,the introduction of carbon regulated the recombination and desorption mode of hydrogen on the surface of metallic Ru,prompting hydrogen to desorb in the form of H2instead of forming H2O.This study provides a new insight for the design and development of catalysts for low-pressure ammonia synthesis.
基金supported by the Ministry of Science and Technology of the People’s Republic of China(2022YFD1800400).
摘要Staphylococcus aureus(S.aureus)is the third most common pathogen causing 10.6%of bacterial foodborne illnesses in China in 2021[1].Heat-stable Staphylococcal Enterotoxins(SEs)produced by S.aureus are the main contributors to staphylococcal food poisoning(SFP),causing vomiting,diarrhea,abdominal pain,headache,muscle cramps,and other acute gastroenteritis symptoms.More than 25 SEs and staphylococcal enterotoxin-like toxins(SE/s)have been described and which together comprise a superfamily of pyrogenic toxin superantigens(SAgs)[2].
摘要The performance of deep recommendation models degrades significantly under data poisoning attacks.While adversarial training methods such as Vulnerability-Aware Training(VAT)enhance robustness by injecting perturbations into embeddings,they remain limited by coarse-grained noise and a static defense strategy,leaving models susceptible to adaptive attacks.This study proposes a novel framework,Self-Purification Data Sanitization(SPD),which integrates vulnerability-aware adversarial training with dynamic label correction.Specifically,SPD first identifies high-risk users through a fragility scoring mechanism,then applies self-purification by replacing suspicious interactions with model-predicted high-confidence labels during training.This closed-loop process continuously sanitizes the training data and breaks the protection ceiling of conventional adversarial training.Experiments demonstrate that SPD significantly improves the robustness of both Matrix Factorization(MF)and LightGCN models against various poisoning attacks.We show that SPD effectively suppresses malicious gradient propagation and maintains recommendation accuracy.Evaluations on Gowalla and Yelp2018 confirmthat SPD-trainedmodels withstandmultiple attack strategies—including Random,Bandwagon,DP,and Rev attacks—while preserving performance.
基金funded by the Medical Science Research Project of the Hebei Provincial Health and Health Commission(20230550).
摘要BACKGROUND:This study is to evaluate clearance eff ects of hemoperfusion(HP),continuous renal replacement therapy(CRRT),and plasma exchange(PE)for chlorfenapyr and its metabolite tralopyril in patients with acute poisoning.METHODS:This retrospective study included 18 patients with acute oral chlorfenapyr poisoning treated at our department between January 2022 and January 2024.All patients received conventional therapies combined with blood purification,including HP,CRRT,and PE.HP was performed three sessions within the fi rst 24 h,followed by CRRT and PE.Serial blood samples were collected to measure plasma concentrations of chlorfenapyr and tralopyril using gas chromatography/liquid chromatography-mass spectrometry(GC/LC-MS).The toxin-clearance eff ects were assessed using a linear mixed-eff ects(LME)model.RESULTS:The hourly decline rate of the plasma chlorfenapyr concentration(median[IQR])was 8.83%(1.79%)for HP,4.12%(1.26%)for CRRT,and 6.85%(1.44%)for PE.LME analysis showed higher decline rate in the plasma concentration with HP(β=5.00;P<0.001)and PE(β=2.15;P=0.003)compared to CRRT.For tralopyril,the hourly decline rates were 3.04%(0.62%)for HP,1.82%(0.48%)for CRRT,and 3.01%(0.37%)for PE.LME analysis showed that the clearance effects of HP(β=0.027;P<0.001)and PE(β=0.022;P=0.001)were superior to CRRT.Pre-treatment toxin levels and the interval from hospital admission to blood purifi cation showed no signifi cant interaction with clearance outcomes.CONCLUSION:In our study,HP was associated with a higher decline rate in plasma chlorfenapyr concentration compared to CRRT and PE,supporting HP as a preferred early intervention.However,all three methods showed limited effi cacy in reducing tralopyril levels.Further research into the toxicokinetics and mechanisms of chlorfenapyr is warranted to optimize purifi cation strategies.
摘要In some cases, poisonous contaminants may be released from bottom sludge inopen channels. The equation of advection and diffusion for the related problem was analyzed in thispaper. The conditions for the definite solution to the equation were given. The analytic solution ofpoisonous gas concentration distribution was worked out. The reasonableness of this solution wasdiscussed. The result is also of significance for other similar problems.