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Novel protein detection method based on proximity-dependent polymerase reaction and aptamers 认领 引用 被引量：3
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作者 YANG XiaoHai WANG Lei +4 位作者 WANG KeMin TAN WeiHong TANG HongXing MENG XiangXian GUO QiuPing 《Chinese Science Bulletin》 2008年第2期204-208,共5页
In recent years,specific detection of proteins is one of the hot issues about aptamers in proteomics.Here we reported a simple,sensitive and specific proximity-dependent protein assay with dual DNA aptamers.Thrombin w... In recent years,specific detection of proteins is one of the hot issues about aptamers in proteomics.Here we reported a simple,sensitive and specific proximity-dependent protein assay with dual DNA aptamers.Thrombin was used as the model protein,and two aptamer probes with complementary se-quence at 3′-end were designed for the two distinct epitopes of the protein.Association of the two ap-tamers with thrombin resulted in stable hybrids due to the proximity of 3′-end,then polymerase reac-tion was induced.The amount of obtained dsDNA was indicated using the fluorescence dye Sybr Green I.The results showed that the initial velocity of polymerase reaction had a positive correlation with concentration of thrombin.The advantages of this dual-aptamer-based approach included simple and flexible design of aptamer probes,high selectivity and high sensitivity.The detection limit was 6.9 pmol/L. 展开更多
关键词 aptamer thrombin polymerase reaction protein detection
Multiple reaction monitoring for the detection of disease-related synaptic proteins 认领 引用
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作者 Rachel Yoon Kyung Chang Peter R.Dodd 《Neural Regeneration Research》 SCIE CAS CSCD 2014年第23期2042-2043,共2页
Synaptic dysfunction occurs early in Alzheimer＇s disease （AD） and is acknowledged as a primary pathologic target for treatment. Synaptic degeneration is the pathological feature most strongly correlated with loss o... Synaptic dysfunction occurs early in Alzheimer＇s disease （AD） and is acknowledged as a primary pathologic target for treatment. Synaptic degeneration is the pathological feature most strongly correlated with loss of cognitive function ante mortern （Terry et al., 1991）. Synapses are heavily damaged in hippocampal and neocortical regions of AD brain, whereas motor and occipital cortices are relatively spared （Honer et al., 1992）. Despite extensive work, the molecular mechanisms underlying synaptic degeneration are largely unknown. 展开更多
关键词 MRM Multiple reaction monitoring for the detection of disease-related synaptic proteins
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Gold Nanoparticles/Thermochromic Composite Film on Screen-Printed Electrodes for Simultaneous Detection of Protein and Temperature 认领 引用 被引量：1
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作者 Dorothy Araba Yakoba Agyapong Hanjia Jiang +2 位作者 Xingjia Ni Jingwen Wu Hongjuan Zeng 《Journal of Biomaterials and Nanobiotechnology》 2021年第2期7-19,共13页
In this study, gold nanoparticles and thermochromic composite films modified screen-printed carbon electrodes (TM-AuNPsSPCEs) were developed as a platform for the simultaneous detection of protein and temperature. The... In this study, gold nanoparticles and thermochromic composite films modified screen-printed carbon electrodes (TM-AuNPsSPCEs) were developed as a platform for the simultaneous detection of protein and temperature. The TM-AuNPs composited film had better sensitivity resulting from the gold nanoparticles amplification effect. A phase transition model analysis of TM-AuNPs films found that the TM-AuNPs films had three-phase transition intervals (80℃) which accommodated the temperature requirements for protein denaturation. When used to detect different concentrations of haemoglobin (Hb) solution, the TM-AuNPs modified SPCEs had a better sensitivity in detecting the different concentrations in comparison to TM and AuNP modified SPCEs which showed no clear sensitivity towards the different Hb concentrations. The dual detection and excellent sensitivity show a good application prospect for the study of the TM-AuNPs composite film. 展开更多
关键词 Screen-Printed Carbon Electrodes Gold Nanoparticles Thermochromic Material Simultaneous Detection of Proteins and Temperature
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Electrochemical aptasensor for the detection of vascular endothelial growth factor(VEGF) based on DNA-templated Ag/Pt bimetallic nanoclusters 认领 引用 被引量：13
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作者 Xian-Ming Fu Zhi-Jing Liu +4 位作者 Shu-Xian Cai Yan-Ping Zhao Dong-Zhi Wu Chun-Yan Li Jing-Hua Chen 《Chinese Chemical Letters》 SCIE CAS CSCD 2016年第6期920-926,共7页
In this paper, the DNA-templated Ag/Pt bimetallic nanoclusters were successfully synthesized using an optimized synthetic scheme. The obtained DNA-Ag/Pt NCs have an ultrasmall particle size and excellent distribution.... In this paper, the DNA-templated Ag/Pt bimetallic nanoclusters were successfully synthesized using an optimized synthetic scheme. The obtained DNA-Ag/Pt NCs have an ultrasmall particle size and excellent distribution. The DNA-Ag/Pt NCs show intrinsic peroxidase-mimicking activity and can effectively catalyze the H2O2-mediated oxidation of a substrate, 3,30,5,50-tetramethylbenzidine（TMB）, to produce a blue colored product. Based on this specific property, we employed the aptamer of VEGF to design a label-free electrochemical biosensor for VEGF detection. Under the optimized experimental conditions, a linear range from 6.0 pmol/L to 20 pmol/L was obtained with a detection limit of 4.6 pmol/L. The proposed biosensor demonstrated its high specificity for VEGF and could directly detect the VEGF concentration in human serum samples of breast cancer patients with satisfactory results. This novel electrochemical aptasensor was simple and convenient to use and was cost-effective and label-free in design, and would hold potential applications in medical diagnosis and treatment. 展开更多
关键词 Vascular endothelial growth factor（VEGF） DNA-templated Ag/Pt bimetallic nanoclusters Enzyme mimics Aptamer Electrochemical aptasensor Protein detection
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Electrochemical detection of human ferritin based on gold nanorod reporter probe and cotton thread immunoassay device 认领 引用 被引量：3
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作者 Ting-Ting Song Wei Wang +3 位作者 Li-Li Meng Yan Liu Xiao-Bo Jia Xun Mao 《Chinese Chemical Letters》 SCIE CAS CSCD 2017年第2期226-230,共5页
In this study,a natural cotton thread immunoassay device combined with gold nanorod（GNR） reporter probe is developed for the rapid,sensitive and quantitative electrochemical determination of human ferritin,a lung ca... In this study,a natural cotton thread immunoassay device combined with gold nanorod（GNR） reporter probe is developed for the rapid,sensitive and quantitative electrochemical determination of human ferritin,a lung cancer related biomarker.Human ferritin as an analyte and a pair of monoclonal antibodies are used to demonstrate the proof-of-concept on the cotton thread immunoassay device.An enhancement of the sensitivity is achieved by using gold nanorod as an electroactive report probe compared with a traditional gold nanoparticle（GNP） report probe.The device was capable of measuring 1.58 ng/mL ferritin in 30 min by anodic stripping voltammetry（ASV） testing,which meet the requirement for clinical diagnosis. 展开更多
关键词 Cotton thread immunoassay device Gold nanorod reporter probe Point-of-care diagnosis Protein detection Electrochemical immunoassay
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Protein expression and antigenicity detection of hepatitis C virus envelope protein E2 认领 引用
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《中国输血杂志》 CAS 2001年第S1期
关键词 Protein expression and antigenicity detection of hepatitis C virus envelope protein E2
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Rhodamine B doped silica nanoparticle labels for protein microarray detection 认领 引用 被引量：3
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作者 WANG Ying LI ZhongHui +3 位作者 ZHONG WenYing LI Hui XU DanKe CHEN HongYuan 《Science China Chemistry》 SCIE EI CAS 2010年第4期747-751,共5页
A core-shell Rhodamine B-doped SiO2 nanoparticle was synthesized and its fluorescent intensity was found to be 1000 times higher than that of individual Rhodamine B molecule.The doped nanoparticles were further conjug... A core-shell Rhodamine B-doped SiO2 nanoparticle was synthesized and its fluorescent intensity was found to be 1000 times higher than that of individual Rhodamine B molecule.The doped nanoparticles were further conjugated with streptavidin and the resulting nanoparticles were used in the detection of reverse-phase protein microarrays,in which human IgG of various concentrations was first immobilized on aldehyde-modified glass slides and then biotinlyated goat anti human IgG as well as the labeled nanoparticles were sequentially conjugated.The calibration curve is linear over the range from 800 fg to 500 pg and the limit of detection is 100 fg,which is 8 times lower than that of streptavidin-labeled Cy3 fluorescent dyes.The dyedoped SiO2 nanoparticles show potentials for the protein array detection. 展开更多
关键词 Rhodamine B SiO2nanoparticles protein detection protein microarrays
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Aptamer-based cell-free detection system to detect target protein 认领 引用 被引量：2
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作者 Junhong Chen Xiaoyan Zhuang +4 位作者 Jiyang Zheng Ruofan Yang Fei Wu Aihui Zhang Baishan Fang 《Synthetic and Systems Biotechnology》 SCIE CSCD 2021年第3期209-215,共7页
Biomarkers of disease,especially protein,show great potential for diagnosis and prognosis.For detecting a certain protein,a binding assay implementing antibodies is commonly performed.However,antibodies are not therma... Biomarkers of disease,especially protein,show great potential for diagnosis and prognosis.For detecting a certain protein,a binding assay implementing antibodies is commonly performed.However,antibodies are not thermally stable and may cause false-positive when the sample composition is complicated.In recent years,a functional nucleic acid named aptamer has been used in many biochemical analysis cases,which is commonly selected from random sequence libraries by using the systematic evolution of ligands by exponential enrichment(SELEX)techniques.Compared to antibodies,the aptamer is more thermal stable,easier to be modified,conjugated,and amplified.Herein,an Aptamer-Based Cell-free Detection(ABCD)system was proposed to detect target protein,using epithelial cell adhesion molecule(EpCAM)as an example.We combined the robustness of aptamer in binding specificity with the signal amplification ability of CRISPR-Cas12a′s trans-cleavage activity in the ABCD system.We also demonstrated that the ABCD system could work well to detect target protein in a relatively low limit of detection(50-100 nM),which lay a foundation for the development of portable detection devices.This work highlights the superiority of the ABCD system in detecting target protein with low abundance and offers new enlightenment for future design and development. 展开更多
关键词 Aptamer CRISPR-Cas Protein detection Biomarker
Comparison between Two Methods for Detecting Gene Flow and Analysis on the Influencing Factors 认领 引用 被引量：2
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作者 贺娟 朱家林 +1 位作者 刘小侠 张青文 《Agricultural Science & Technology》 CAS 2011年第6期840-841,861,共2页
[Objective] The aim was to test the consistency between two commonly used methods PCR detection and protein detection for detecting gene flow,and investigate the factors influencing the consistency.[Method] 1 769 samp... [Objective] The aim was to test the consistency between two commonly used methods PCR detection and protein detection for detecting gene flow,and investigate the factors influencing the consistency.[Method] 1 769 samples of three varieties under three treatments（wind,bee and control）were detected with both methods.[Result] There was phenomenon in field that Bt gene was transferred into F1 generations but couldn＇t express,that meant,the result of PCR detection was not consistent with that of protein detection.By comparing environmental factors,it was proved that wind and bee treatments didn＇t significantly affect the expression of gene flow.However,the Bt gene expression rate in bee treatment was higher than that in wind treatment.[Conclusion] The paper will provide reference for accurate detection of gene flow. 展开更多
关键词 Gene flow PCR Protein detection
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Enzyme-catalyzed deposition of polydopamine for amplifying the signal inhibition to a novel Prussian blue-nanocomposite and ultrasensitive electrochemical immunosensing 认领 引用 被引量：1
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作者 Wan Huang Peng Guo +4 位作者 Bo Li Li Fu Cheng-Te Lin Aimin Yu Guosong Lai 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2022年第7期166-173,共8页
The uncontrollable synthesis of Prussian blue (PB) and its weak stability toward OH -are great challenges affecting its electrochemical biosensing application. Herein we utilize the unique properties of chitosan (CS) ... The uncontrollable synthesis of Prussian blue (PB) and its weak stability toward OH -are great challenges affecting its electrochemical biosensing application. Herein we utilize the unique properties of chitosan (CS) to realize the facile and controllable synthesis of a CS-PB nanocomposite and combine it with the urease-catalyzed deposition of polydopamine (PDA) for amplifying the electrochemical signal inhibition of PB to develop a novel immunosensing method for protein detection. The immunosensor was constructed on a CS-PB modified electrode, and a urease-functionalized silica nanoprobe was prepared for tracing its sandwich immunoassay toward the model analyte of carcinoembryonic antigen. Besides the electrochem- ical impedance effect of the quantitatively captured nanoprobes, their enzymatic reaction can release numerous OH -to destroy the PB crystals and also induce the PDA deposition onto the immunosensor. These caused drastic electrochemical signal inhibition to PB. Based on the above multi-signal amplification mechanism, the method exhibits a very low detection limit of 0.042 pg mL-1 along with a very wide linear range of six-order of magnitude. In addition, the CS-PB based immunosensor has excellent specificity, repeatability, stability and reliability. Thus this PB nanocomposite and the proposed electrochemical immunosensing method reveal a promising potential for future applications. 展开更多
关键词 Prussian blue Polydopamine Biosensor Electroanalytical chemistry Protein detection
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Hydrophilic modification enables a performance breakthrough in the conventional metallic metasurface 认领 引用
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作者 SHUOCHENG SHE YANG LI +6 位作者 JIACHEN ZUO CAIQIN ZHAO ZEKUN YANG YAOWEI DAI QIUHONG QU YIZHU ZHANG MINGXIA HE 《Photonics Research》 SCIE EI CAS CSCD 2026年第1期11-21,共11页
Metasurfaces,as advanced photonic platforms capable of precise electromagnetic manipulation,confine optical energy within meta-atoms,generating intense near-field hotspots that greatly enhance light–matter interactio... Metasurfaces,as advanced photonic platforms capable of precise electromagnetic manipulation,confine optical energy within meta-atoms,generating intense near-field hotspots that greatly enhance light–matter interactions and enable ultrasensitive biosensing.However,the superhydrophobicity of gold leads to a pronounced coffee-ring effect during the evaporation of liquid samples,resulting in uneven distribution of biomolecules and weakened light–matter interactions.Herein,we present a metallic terahertz(THz)metasurface sensor whose metal array region is modified with ionic liquids(ILs),resulting in a reduction of the droplet contact angle from 105.7°to 39.4°.This indicates excellent hydrophilicity,which facilitates the uniform deposition of protein molecules on the surface.This strategy circumvents the conventional dependence on high quality(Q)factor resonances and delivers outstanding sensing performance.Specifically,under y-polarized THz excitation,the two resonance modes excited by the metasurface enable reliable qualitative and quantitative detection of various proteins.Among these modes,Mode II exhibited a direct limit of detection(LoD)of 0.03125 mg/mL for insulin.This work establishes a new paradigm for trace biomolecule sensing and provides valuable insights for future sensor innovations. 展开更多
关键词 ionic liquids biosensing precise electromagnetic manipulationconfine hydrophilic modification terahertz metasurface protein detection enhance light matter interactions coffee ring effect
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Highly Sensitive Protein Sensor Based on Thermally-Reduced Graphene Oxide Field-Effect Transistor 认领 引用 被引量：6
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作者 Shun Mao Kehan Yu +1 位作者 Ganhua Lu Junhong Chen 《Nano Research》 SCIE CSCD 2011年第10期921-930,共10页
We report the fabrication of a highly sensitive field-effect transistor(FET)biosensor using thermally-reduced graphene oxide(TRGO)sheets functionalized with gold nanoparticle(NP)-antibody conjugates.Probe antibody was... We report the fabrication of a highly sensitive field-effect transistor(FET)biosensor using thermally-reduced graphene oxide(TRGO)sheets functionalized with gold nanoparticle(NP)-antibody conjugates.Probe antibody was labeled on the surface of TRGO sheets through Au NPs and electrical detection of protein binding(Immunoglobulin G/IgG and anti-lmmunoglobulin G/anti-lgG)was accomplished by FET and direct current(dc)measurements.The protein binding events induced significant changes in the resistance of the TRGO sheet,which is referred to as the sensor response.The dependence of the sensor response on the TRGO base resistance in the sensor and the antibody areal density on the TRGO sheet was systematically studied,from which a correlation of the sensor response with sensor parameters was found:the sensor response was more significant with larger TRGO base resistance and higher antibody areal density.The detection limit of the novel biosensor was around the 0.2 ngnL level,which is among the best of,'eported carbon nanomaterial-based protein sensors and can be further optimized by tuning the sensor structure. 展开更多
关键词 Biosensor thermally-reduced graphene oxide field-effect transistor protein detection
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Crosstalk-free colloidosomes for high throughput single-molecule protein analysis 认领 引用
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作者 Kun Yin Xi Zeng +7 位作者 Xuan Liang Haopai Wei Huimin Zeng Wei Qi Weidong Ruan Yanling Song Chaoyong Yang Zhi Zhu 《Science China Chemistry》 SCIE EI CAS CSCD 2020年第10期1507-1514,共8页
Droplet microfluidics is a powerful platform for high-throughput single-molecule protein analysis.However,the issues of coalescence and crosstalk of droplets compromise the accuracy of detection and hinder its wide ap... Droplet microfluidics is a powerful platform for high-throughput single-molecule protein analysis.However,the issues of coalescence and crosstalk of droplets compromise the accuracy of detection and hinder its wide application.To address these limitations,a novel colloidosome-based method was presented by combining a Pickering emulsion with droplet microfluidics for single-molecule protein analysis.Utilizing the self-assembly of easily synthesized colloidal surfactant F-SiO2 NPs at the water/oil interface,the colloidosomes are rigidly stabilized and can effectively avoid the leakage of fluorescent molecules.The crosstalk-free colloidosomes enable high-throughput single-molecule protein analysis,including heterogenous dynamic studies and digital detection.As a robust and accurate method,colloidosome-based microfluidics is promising as a powerful tool for a wide variety of applications,such as directed enzyme evolution,digital enzyme-linked immunosorbent assay(ELISA),and screening of antibiotics. 展开更多
关键词 colloidosomes droplet microfluidics crosstalk-free single-molecule analysis protein detection
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CPL:Detecting Protein Complexes by Propagating Labels on Protein-Protein Interaction Network 认领 引用 被引量：2
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作者 代启国 郭茂祖 +2 位作者 刘晓燕 滕志霞 王春宇 《Journal of Computer Science & Technology》 SCIE EI CSCD 2014年第6期1083-1093,共11页
Proteins usually bind together to form complexes, which play an important role in cellular activities. Many graph clustering methods have been proposed to identify protein complexes by finding dense regions in protein... Proteins usually bind together to form complexes, which play an important role in cellular activities. Many graph clustering methods have been proposed to identify protein complexes by finding dense regions in protein-protein interaction networks. We present a novel framework （CPL） that detects protein complexes by propagating labels through interactions in a network, in which labels denote complex identifiers. With proper propagation in CPL, proteins in the same complex will be assigned with the same labels. CPL does not make any strong assumptions about the topological structures of the complexes, as in previous methods. Tile CPL algorithm is tested on several publicly available yeast protein-protein interaction networks and compared with several state-of-the-art methods. The results suggest that CPL performs better than the existing methods. An analysis of the functional homogeneity based on a gene ontology analysis shows that the detected complexes of CPL are highly biologically relevant. 展开更多
关键词 protein complex detection label propagation protein-protein interaction graph clustering bioinformatics
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Phase-sensitive plasmonic biosensor using a portable and large field-of-view interferometric microarray imager 认领 引用 被引量：10
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作者 Filiz Yesilkoy Roland A Terborg +4 位作者 Josselin Pello Alexander A Belushkin Yasaman Jahani Valerio Pruneri Hatice Altug 《Light: Science & Applications》 SCIE EI CSCD 2017年第1期159-167,共9页
Nanophotonics,and more specifically plasmonics,provides a rich toolbox for biomolecular sensing,since the engineered metasurfaces can enhance light–matter interactions to unprecedented levels.So far,biosensing associ... Nanophotonics,and more specifically plasmonics,provides a rich toolbox for biomolecular sensing,since the engineered metasurfaces can enhance light–matter interactions to unprecedented levels.So far,biosensing associated with high-quality factor plasmonic resonances has almost exclusively relied on detection of spectral shifts and their associated intensity changes.However,the phase response of the plasmonic resonances have rarely been exploited,mainly because this requires a more sophisticated optical arrangement.Here we present a new phase-sensitive platform for high-throughput and label-free biosensing enhanced by plasmonics.It employs specifically designed Au nanohole arrays and a large field-of-view interferometric lens-free imaging reader operating in a collinear optical path configuration.This unique combination allows the detection of atomically thin(angstrom-level)topographical features over large areas,enabling simultaneous reading of thousands of microarray elements.As the plasmonic chips are fabricated using scalable techniques and the imaging reader is built with low-cost off-the-shelf consumer electronic and optical components,the proposed platform is ideal for point-of-care ultrasensitive biomarker detection from small sample volumes.Our research opens new horizons for on-site disease diagnostics and remote health monitoring. 展开更多
关键词 interferometric imaging label-free plasmonic biosensors lens-free imaging phase interrogation point-of-care devices protein microarray detection
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