Biological insecticides have gained importance in modern society because they cause minimal environmental harm and reduce pests’ability to develop resistance.Nomuraea rileyi,a facultative entomopathogenic fungus,is s...Biological insecticides have gained importance in modern society because they cause minimal environmental harm and reduce pests’ability to develop resistance.Nomuraea rileyi,a facultative entomopathogenic fungus,is suitable for infecting lepidopteran pests,including Spodoptera litura and Helicoverpa armigera.This work aims to identify a suitable semi-solid fermentation medium to increase the conidia production of N.rileyi and enhance its biological control ability as mycoinsecticide.Research is carried out on broken rice,wheat,sorghum,and corn as substrates,both with and without nutrient addition.It also covers incubation under set conditions,colony harvesting,and conidial yield and germination determination using a haemocytometer.All the study was conducted using a completely randomized design(CRD)and a test of significance was set at p<0.05 using analysis of variance(ANOVA).This work aimed to establish the effect of various substrates on growth and conidia formation in N.rileyi.The highest conidia count of 10×108CFU/g on day 15.The result was obtained from BR+Nu followed by sorghum 7×108CFU/g,corn 7×108CFU/g and wheat 6.5×108CFU/g.Thus,post-drying,the highest corresponding viability was observed in the product containing BR+Nu–6.20×108CFU/g.Adding rice bran(BR+RB,150 g each)still increases conidial yield up to 14×108 CFU/g at 15 DAI.These indings thus assert that broken rice and supplementation affect conidia yield and viability.展开更多
F-box proteins are components of the SCF (SkpA-Cullin 1-F-box) E3 ligase complexes, acting as the specificity-determinants in targeting substrate proteins for ubiquitination and degradation. In humans, at least 22 o...F-box proteins are components of the SCF (SkpA-Cullin 1-F-box) E3 ligase complexes, acting as the specificity-determinants in targeting substrate proteins for ubiquitination and degradation. In humans, at least 22 out of 75 F-box proteins have experimentally documented substrates, whereas in Drosophila 12 F-box proteins have been characterized with known substrates. To systematically investigate the genetic and molecular functions of F-box proteins in Drosophila, we performed a survey of the literature and databases. We identified 45 Drosophila genes that encode proteins containing at least one F-box domain. We collected publically available RNAi lines against these genes and used them in a tissue-specific RNAi-based phenotypic screen. Here, we present our systematic phenotypic dataset from the eye, the wing and the notum. This dataset is the first of its kind and represents a useful resource for future studies of the molecular and genetic functions of F-box genes in Drosophila. Our results show that, as expected, F-box genes in Drosophila have regulatory roles in a diverse array of processes including cell proliferation, cell growth, signal transduction, and cellular and animal survival.展开更多
The green miridbug,Cyrtorhinus lividipennis,is an important predator of the rice brown planthopper,Nilaparvata lugens.In this study,the selective toxicity of seven commercial biopesticides for C.lividipennis was exami...The green miridbug,Cyrtorhinus lividipennis,is an important predator of the rice brown planthopper,Nilaparvata lugens.In this study,the selective toxicity of seven commercial biopesticides for C.lividipennis was examined under laboratory conditions; abamectin was the most selective to C.lividipennis,followed by matrine and azadirachtin.Veratridine,rotenone,Bacillus thuringiensis and Beauveria bassiana showed less selectivity for C.lividipennis.Subsequently,matrine,abamectin and azadirachtin were selected for sublethal assessments with respect to C.lividipennis due to their high toxicities to N.lugens.C.lividipennis treated with sublethal concentrations(LC10 and LC20) of the three biopesticides could distinguish volatiles released from healthy and N.lugens-infested plants indicating that the biopesticides tested did not affect the foraging ability of surviving miridbugs.Azadirachtin decreased the consumption capability of C.lividipennis when the densities of N.lugens were 20,30,40 and 50 insects per vial.Sublethal concentration treatment did not impact the pre-oviposition period or egg hatchability of C.lividipennis.However,the fecundity of C.lividipennis exposed to azadirachtin and abamectin increased by 27–41% compared to the untreated individuals.In summary,abamectin or matrine together with C.lividipennis could be considered an effective,sustainable pest management strategy for rice.展开更多
RNA interference(RNAi)represents one of the most conserved pathways evolved by eukaryotic cells for regulating gene expression.RNAi utilises non-translatable double-stranded RNA(dsRNA)molecules to sequester or degrade...RNA interference(RNAi)represents one of the most conserved pathways evolved by eukaryotic cells for regulating gene expression.RNAi utilises non-translatable double-stranded RNA(dsRNA)molecules to sequester or degrade mRNA molecules gene.In RNAi,specifically designed exogenous dsRNA delivered to the cell can silence a target gene,a phenomenon that has been exploited in many functional studies and explored in biopesticide applications.The search for safe and sustainable crop pest management options drives the need to offset the effect of inorganic pesticides on biodiversity.The prospect of replacing inorganic pesticides with dsRNA crop spray is gaining popularity,enhanced by its high-target specificity and low environmental impact.However,for dsRNA to reach the pesticide market,it must be produced cost-effectively and sustainably.In this paper,we develop a high-yield expression media that generates up to 15-fold dsRNA yield compared to existing expression media utilising 1 mM IPTG.We also optimise a low-cost purification method that generates high-quality and purified dsRNA.The developed method circumvents the need for hazardous chemical reagents often found in commercial kits or commercial nucleases to eliminate contaminating DNA or single-stranded RNA(ssRNA)species.We also demonstrate that the production platform is scalable,generating 6.29 mg dsRNA from 259 mg wet E.colicell pellet.The results also provide structural insights into the heterogeneous dsRNA species within the microbial-derived dsRNA pool.Finally,we also show that the purified‘naked’dsRNA,without prior formulation,can induce insect toxicity under field conditions.This study provides a novel,complete,low-cost process dsRNA platform with potential for application in industrial dsRNA production.展开更多
In this study, toxicity and control efficiency of three nicotine biopesticides against pomegranate aphids were determined via indoor toxicity test and field effica- cy test, aiming at screening the appropriate reagent...In this study, toxicity and control efficiency of three nicotine biopesticides against pomegranate aphids were determined via indoor toxicity test and field effica- cy test, aiming at screening the appropriate reagent for prevention and control of pomegranate aphids. The results of indoor toxicity test showed that LC^o of reagent 1, reagent 2 and reagent 3 reached 10 270, 12 810 and 13 040 mg/L, respectively. The results of field efficacy test showed that control efficacy of reagent 1, reagent 2 and reagent 3 reached 60.52%, 34.60% and 46.99%, respectively. Overall, reagent 1 (91% ultrafine powder of tobacco leaves, 3% silicone oil, 2% sasangua cake, 2% emulsifier, 2% ascorbic acid) exhibited the highest control efficiency against pomegranate aphids, which can be further popularized and utilized.展开更多
Biopesticides are derivatives of plants, microorganisms and insects. Substances from plants and animals have been used to manage diseases in crops, animals and humans. Reliance on nature to heal nature is a practise f...Biopesticides are derivatives of plants, microorganisms and insects. Substances from plants and animals have been used to manage diseases in crops, animals and humans. Reliance on nature to heal nature is a practise for many people around the world. Use of natural products was overtaken by synthetic chemicals due to their efficacy, reliability and quick knock down effect. However, synthetic pesticides have become a health hazards for humans and environment due to their toxicity and pollution. Biopesticides are potential alternatives to synthetic pesticides. Sources of biopesticides are readily available, easily biodegradable, exhibit various modes of action, are less expensive and have low toxicity to humans and non-target organisms. Neem, pyrethrum, cotton and tobacco are known sources of botanical pesticides and have already been commercialized. Other sources of botanical pesticides include garlic, euphorbia, citrus, pepper among others. Species of Trichoderma, Bacillus, Pseudomonas, Beauveria have been commercialized as microbial pesticides. Biopesticides are however faced with challenges of formulation, registration, commercialization, acceptance and adoption. This paper describes several aspects of biopesticide development, including but not limited to, their sources, production, formulation, commercialization, efficacy and role in sustainable agriculture.展开更多
Neem extract is an alternative insecticide for organic farming and is an allowed component for commercial biopesticide in Korea. However, crop protection properties are not consistent in commercial products. In this r...Neem extract is an alternative insecticide for organic farming and is an allowed component for commercial biopesticide in Korea. However, crop protection properties are not consistent in commercial products. In this report, quantitative analysis of commercial biopesticides for the four insecticidal limonoids of neem extract-azadirachtin A, azadirachtin B, deacetylsalannin and salannin, was conducted through solid phase extraction method with lipophilic-hydrophilic balanced material. The recoveries of the four limonoids ranged from 80.5% to 105%, and their limit of quantitation ranged from 0.028 mg/L to 0.356 mg/L. On the five imported neem extracts, the total contents of the four bioactive limonoids extracted were from 321 mg/L to 5810 mg/L, but there were big variations in the relative composition of the limonoids. The total limonoidal concentrations in 23 commercial bio-pesticides made from neem showed from below LOQ to 7190 mg/L with significant differences in the relative composition. These differences determine the biopesticide’s efficacy on pests, therefore, tracking the active ingredients is necessary for the quality control of commercial bio-pesticides.展开更多
Successive cartridge clean-up method for the simultaneous determination of matrine and oxyma- trine in biopesticides containing Sophora flavescens extract was developed and validated by UPLC. The clean-up method was e...Successive cartridge clean-up method for the simultaneous determination of matrine and oxyma- trine in biopesticides containing Sophora flavescens extract was developed and validated by UPLC. The clean-up method was established with ENVI-Carb (0.5 g) and C18 SPE (0.5 g) cartridges for the bioactive alkaloid in biopesticides from S. flavescens, and the eluate was analyzed to quantify the matrine and oxymatrine by UPLC. The developed method was validated, and the recovery and LOQ of both materials were 105.0% and 103.6%, and 0.050 and 0.684 mg·kg-1, respectively. Of the twenty one samples, the total content of matrines were analyzed by using the developed method and the result showed the developed successive clean-up method could contribute to the manufacture and control of biopesticides including matrines, and can be ap- plied to the method development for the analysis of alkaloid materials in biopesticides.展开更多
[Objectives]The paper was to search for biopesticides with good control effect on grape gray mold.[Methods]A randomized field trial was carried out to compare the control effects of 100 million CFU/g Trichoderma harzi...[Objectives]The paper was to search for biopesticides with good control effect on grape gray mold.[Methods]A randomized field trial was carried out to compare the control effects of 100 million CFU/g Trichoderma harzianum WDG,200 million spores/g Trichoderma WP,24% validamycin AS,0.3% matrine AS on grape gray mold.[Results]Of the 4 biopesticides,200 million spores/g Trichoderma WP had the highest control effect on grape gray mold,with the control effect of 73.38% at 7 d post the second application,significantly higher than those of 100 million CFU/g T.harzianum WG and 0.3% matrine AS;the control effect was 75.77% at 14 d post the second application,significantly higher than those of other 3 biopesticides.[Conclusions]The 200 million spores/g Trichoderma WP shows a good control effect on grape gray mold with a long-lasting control effect,so it is more is for the control of summer black grape gray mold in Jianshui County.展开更多
Cotton production faces significant challenges from insect pests,with chemical pesticide use becoming increasingly limited by resistance and environmental concerns.This study explores the potential use of caffeine,a n...Cotton production faces significant challenges from insect pests,with chemical pesticide use becoming increasingly limited by resistance and environmental concerns.This study explores the potential use of caffeine,a natural plant alkaloid,as an environmentally friendly insect resistance strategy in cotton.Exogenous caffeine application demonstrated potent insecticidal effects against cotton bollworm(Helicoverpa armigera)larvae,with concentrations≥2 mg mL−1 causing near-complete feeding cessation and up to 70%larval mortality.Building on this,we engineered transgenic cotton(Gossypium hirsutum cv.Jin668)for heterologous caffeine biosynthesis by introducing three key N-methyltransferase genes(CaXMT1,CaMXMT1,CaDXMT1)by multiple gene transformation.Transgenic lines expressing all three genes showed remarkable caffeine accumulation(up to 3.59 mg g−1 dry weight),whereas two-gene combinations exhibited wild-type-level production.Feeding preference assays revealed that caffeine-enriched cotton strongly deterred feeding by H.armigera.Non-choice feeding trials demonstrated reduced leaf consumption and reduced larval growth in H.armigera fed on caffeine-producing cotton.The study highlights the effectiveness of synthetic biology approaches using the TGSII-UNiE multigene stacking system,despite challenges in transgene stability.This work advances plant-derived insect resistance research and provides a sustainable framework for reducing chemical pesticide reliance in cotton production,while underscoring unique potential of cotton as a synthetic biology platform for secondary metabolite engineering.展开更多
Agriculture,which serves as the foundation of human civilization,faces threats from multiple sources,including pests,soil erosion,and unpredictable weather patterns.These challenges can affect agricultural productivit...Agriculture,which serves as the foundation of human civilization,faces threats from multiple sources,including pests,soil erosion,and unpredictable weather patterns.These challenges can affect agricultural productivity,food security,and environmental sustainability.Among the solutions,microbial metabolites can provide hope.These secondary metabolites are naturally produced by microorganisms during metabolism and consist of a broad range of compounds with varied roles,acting as biofertilizers,biopesticides,and plant growth promoters and thereby assuring sustainable agriculture.These metabolites help to liberate nutrients and improve soil structure.They are also important biocontrol agents for reducing plant pathogens and pests.These metabolites are essential for nutrient cycling,soil fertility,and crop productivity.There are many advantages to using microbial metabolites in agriculture,such as low dependence on chemical pesticides and fertilizers,increased crop productivity,and improved crop health.The challenges of production,formulation,consistency,and regulatory framework must be resolved before the microbial metabolites can be widely accepted.The future of agriculture will be shaped by advancements in microbial metabolite research,integrated with cutting-edge agricultural technologies and supported by aligned administrative policies.In summary,it is well established that microbial metabolites possess the capacity that significantly transform agriculture.Integrating the inherent potential of natural solutions can help create more sustainable and resilient agricultural systems that can protect food security,promote environmental sustainability,and ensure the future of future generations.展开更多
摘要Biological insecticides have gained importance in modern society because they cause minimal environmental harm and reduce pests’ability to develop resistance.Nomuraea rileyi,a facultative entomopathogenic fungus,is suitable for infecting lepidopteran pests,including Spodoptera litura and Helicoverpa armigera.This work aims to identify a suitable semi-solid fermentation medium to increase the conidia production of N.rileyi and enhance its biological control ability as mycoinsecticide.Research is carried out on broken rice,wheat,sorghum,and corn as substrates,both with and without nutrient addition.It also covers incubation under set conditions,colony harvesting,and conidial yield and germination determination using a haemocytometer.All the study was conducted using a completely randomized design(CRD)and a test of significance was set at p<0.05 using analysis of variance(ANOVA).This work aimed to establish the effect of various substrates on growth and conidia formation in N.rileyi.The highest conidia count of 10×108CFU/g on day 15.The result was obtained from BR+Nu followed by sorghum 7×108CFU/g,corn 7×108CFU/g and wheat 6.5×108CFU/g.Thus,post-drying,the highest corresponding viability was observed in the product containing BR+Nu–6.20×108CFU/g.Adding rice bran(BR+RB,150 g each)still increases conidial yield up to 14×108 CFU/g at 15 DAI.These indings thus assert that broken rice and supplementation affect conidia yield and viability.
基金financially supported by the National Basic Research Program of China(973 Program)(No.2009CB918702)the National Natural Science Foundation of China(Nos.31071087 and 31100889)
摘要F-box proteins are components of the SCF (SkpA-Cullin 1-F-box) E3 ligase complexes, acting as the specificity-determinants in targeting substrate proteins for ubiquitination and degradation. In humans, at least 22 out of 75 F-box proteins have experimentally documented substrates, whereas in Drosophila 12 F-box proteins have been characterized with known substrates. To systematically investigate the genetic and molecular functions of F-box proteins in Drosophila, we performed a survey of the literature and databases. We identified 45 Drosophila genes that encode proteins containing at least one F-box domain. We collected publically available RNAi lines against these genes and used them in a tissue-specific RNAi-based phenotypic screen. Here, we present our systematic phenotypic dataset from the eye, the wing and the notum. This dataset is the first of its kind and represents a useful resource for future studies of the molecular and genetic functions of F-box genes in Drosophila. Our results show that, as expected, F-box genes in Drosophila have regulatory roles in a diverse array of processes including cell proliferation, cell growth, signal transduction, and cellular and animal survival.
基金financially supported by the Jiangsu Agricultural Scientific Self-innovation Fund,China (CX(15)1057)the Fund of Science and Technology of Jiangsu Province,China (BE2015342)the National Key Research and Development Program of China (2017YFD0200400 and 2016YFD0300706)
摘要The green miridbug,Cyrtorhinus lividipennis,is an important predator of the rice brown planthopper,Nilaparvata lugens.In this study,the selective toxicity of seven commercial biopesticides for C.lividipennis was examined under laboratory conditions; abamectin was the most selective to C.lividipennis,followed by matrine and azadirachtin.Veratridine,rotenone,Bacillus thuringiensis and Beauveria bassiana showed less selectivity for C.lividipennis.Subsequently,matrine,abamectin and azadirachtin were selected for sublethal assessments with respect to C.lividipennis due to their high toxicities to N.lugens.C.lividipennis treated with sublethal concentrations(LC10 and LC20) of the three biopesticides could distinguish volatiles released from healthy and N.lugens-infested plants indicating that the biopesticides tested did not affect the foraging ability of surviving miridbugs.Azadirachtin decreased the consumption capability of C.lividipennis when the densities of N.lugens were 20,30,40 and 50 insects per vial.Sublethal concentration treatment did not impact the pre-oviposition period or egg hatchability of C.lividipennis.However,the fecundity of C.lividipennis exposed to azadirachtin and abamectin increased by 27–41% compared to the untreated individuals.In summary,abamectin or matrine together with C.lividipennis could be considered an effective,sustainable pest management strategy for rice.
基金support of the Research England/HEFCEHEIF(QR-GCRF)+2 种基金Royal Society of ChemistryAll First Technologies,Pingzhen Dist,Taoyuan City,Taiwanthe University of Sheffield Institutional Open Access Fund.
摘要RNA interference(RNAi)represents one of the most conserved pathways evolved by eukaryotic cells for regulating gene expression.RNAi utilises non-translatable double-stranded RNA(dsRNA)molecules to sequester or degrade mRNA molecules gene.In RNAi,specifically designed exogenous dsRNA delivered to the cell can silence a target gene,a phenomenon that has been exploited in many functional studies and explored in biopesticide applications.The search for safe and sustainable crop pest management options drives the need to offset the effect of inorganic pesticides on biodiversity.The prospect of replacing inorganic pesticides with dsRNA crop spray is gaining popularity,enhanced by its high-target specificity and low environmental impact.However,for dsRNA to reach the pesticide market,it must be produced cost-effectively and sustainably.In this paper,we develop a high-yield expression media that generates up to 15-fold dsRNA yield compared to existing expression media utilising 1 mM IPTG.We also optimise a low-cost purification method that generates high-quality and purified dsRNA.The developed method circumvents the need for hazardous chemical reagents often found in commercial kits or commercial nucleases to eliminate contaminating DNA or single-stranded RNA(ssRNA)species.We also demonstrate that the production platform is scalable,generating 6.29 mg dsRNA from 259 mg wet E.colicell pellet.The results also provide structural insights into the heterogeneous dsRNA species within the microbial-derived dsRNA pool.Finally,we also show that the purified‘naked’dsRNA,without prior formulation,can induce insect toxicity under field conditions.This study provides a novel,complete,low-cost process dsRNA platform with potential for application in industrial dsRNA production.
摘要In this study, toxicity and control efficiency of three nicotine biopesticides against pomegranate aphids were determined via indoor toxicity test and field effica- cy test, aiming at screening the appropriate reagent for prevention and control of pomegranate aphids. The results of indoor toxicity test showed that LC^o of reagent 1, reagent 2 and reagent 3 reached 10 270, 12 810 and 13 040 mg/L, respectively. The results of field efficacy test showed that control efficacy of reagent 1, reagent 2 and reagent 3 reached 60.52%, 34.60% and 46.99%, respectively. Overall, reagent 1 (91% ultrafine powder of tobacco leaves, 3% silicone oil, 2% sasangua cake, 2% emulsifier, 2% ascorbic acid) exhibited the highest control efficiency against pomegranate aphids, which can be further popularized and utilized.
摘要Biopesticides are derivatives of plants, microorganisms and insects. Substances from plants and animals have been used to manage diseases in crops, animals and humans. Reliance on nature to heal nature is a practise for many people around the world. Use of natural products was overtaken by synthetic chemicals due to their efficacy, reliability and quick knock down effect. However, synthetic pesticides have become a health hazards for humans and environment due to their toxicity and pollution. Biopesticides are potential alternatives to synthetic pesticides. Sources of biopesticides are readily available, easily biodegradable, exhibit various modes of action, are less expensive and have low toxicity to humans and non-target organisms. Neem, pyrethrum, cotton and tobacco are known sources of botanical pesticides and have already been commercialized. Other sources of botanical pesticides include garlic, euphorbia, citrus, pepper among others. Species of Trichoderma, Bacillus, Pseudomonas, Beauveria have been commercialized as microbial pesticides. Biopesticides are however faced with challenges of formulation, registration, commercialization, acceptance and adoption. This paper describes several aspects of biopesticide development, including but not limited to, their sources, production, formulation, commercialization, efficacy and role in sustainable agriculture.
摘要Neem extract is an alternative insecticide for organic farming and is an allowed component for commercial biopesticide in Korea. However, crop protection properties are not consistent in commercial products. In this report, quantitative analysis of commercial biopesticides for the four insecticidal limonoids of neem extract-azadirachtin A, azadirachtin B, deacetylsalannin and salannin, was conducted through solid phase extraction method with lipophilic-hydrophilic balanced material. The recoveries of the four limonoids ranged from 80.5% to 105%, and their limit of quantitation ranged from 0.028 mg/L to 0.356 mg/L. On the five imported neem extracts, the total contents of the four bioactive limonoids extracted were from 321 mg/L to 5810 mg/L, but there were big variations in the relative composition of the limonoids. The total limonoidal concentrations in 23 commercial bio-pesticides made from neem showed from below LOQ to 7190 mg/L with significant differences in the relative composition. These differences determine the biopesticide’s efficacy on pests, therefore, tracking the active ingredients is necessary for the quality control of commercial bio-pesticides.
摘要Successive cartridge clean-up method for the simultaneous determination of matrine and oxyma- trine in biopesticides containing Sophora flavescens extract was developed and validated by UPLC. The clean-up method was established with ENVI-Carb (0.5 g) and C18 SPE (0.5 g) cartridges for the bioactive alkaloid in biopesticides from S. flavescens, and the eluate was analyzed to quantify the matrine and oxymatrine by UPLC. The developed method was validated, and the recovery and LOQ of both materials were 105.0% and 103.6%, and 0.050 and 0.684 mg·kg-1, respectively. Of the twenty one samples, the total content of matrines were analyzed by using the developed method and the result showed the developed successive clean-up method could contribute to the manufacture and control of biopesticides including matrines, and can be ap- plied to the method development for the analysis of alkaloid materials in biopesticides.
基金Supported by Scientific and Technological Innovation Platform Construction Project for Biological Control Resources Exploration and Utilization in Yunnan Province(202207AB110009)。
摘要[Objectives]The paper was to search for biopesticides with good control effect on grape gray mold.[Methods]A randomized field trial was carried out to compare the control effects of 100 million CFU/g Trichoderma harzianum WDG,200 million spores/g Trichoderma WP,24% validamycin AS,0.3% matrine AS on grape gray mold.[Results]Of the 4 biopesticides,200 million spores/g Trichoderma WP had the highest control effect on grape gray mold,with the control effect of 73.38% at 7 d post the second application,significantly higher than those of 100 million CFU/g T.harzianum WG and 0.3% matrine AS;the control effect was 75.77% at 14 d post the second application,significantly higher than those of other 3 biopesticides.[Conclusions]The 200 million spores/g Trichoderma WP shows a good control effect on grape gray mold with a long-lasting control effect,so it is more is for the control of summer black grape gray mold in Jianshui County.
基金supported by the National Natural Science Foundation of China (32325039)
摘要Cotton production faces significant challenges from insect pests,with chemical pesticide use becoming increasingly limited by resistance and environmental concerns.This study explores the potential use of caffeine,a natural plant alkaloid,as an environmentally friendly insect resistance strategy in cotton.Exogenous caffeine application demonstrated potent insecticidal effects against cotton bollworm(Helicoverpa armigera)larvae,with concentrations≥2 mg mL−1 causing near-complete feeding cessation and up to 70%larval mortality.Building on this,we engineered transgenic cotton(Gossypium hirsutum cv.Jin668)for heterologous caffeine biosynthesis by introducing three key N-methyltransferase genes(CaXMT1,CaMXMT1,CaDXMT1)by multiple gene transformation.Transgenic lines expressing all three genes showed remarkable caffeine accumulation(up to 3.59 mg g−1 dry weight),whereas two-gene combinations exhibited wild-type-level production.Feeding preference assays revealed that caffeine-enriched cotton strongly deterred feeding by H.armigera.Non-choice feeding trials demonstrated reduced leaf consumption and reduced larval growth in H.armigera fed on caffeine-producing cotton.The study highlights the effectiveness of synthetic biology approaches using the TGSII-UNiE multigene stacking system,despite challenges in transgene stability.This work advances plant-derived insect resistance research and provides a sustainable framework for reducing chemical pesticide reliance in cotton production,while underscoring unique potential of cotton as a synthetic biology platform for secondary metabolite engineering.
基金the Graphic Era(deemed to be University),Indiathe Invertis University,India+4 种基金the NIMS University,Indiathe Dayalbagh Educational Institute,Indiathe Teerthankar Mahaveer University,Indiathe Siksha‘O’Anusandhan(deemed to be University),Indiathe Instituto Tecnológico de Sonora,Mexico for their support。
摘要Agriculture,which serves as the foundation of human civilization,faces threats from multiple sources,including pests,soil erosion,and unpredictable weather patterns.These challenges can affect agricultural productivity,food security,and environmental sustainability.Among the solutions,microbial metabolites can provide hope.These secondary metabolites are naturally produced by microorganisms during metabolism and consist of a broad range of compounds with varied roles,acting as biofertilizers,biopesticides,and plant growth promoters and thereby assuring sustainable agriculture.These metabolites help to liberate nutrients and improve soil structure.They are also important biocontrol agents for reducing plant pathogens and pests.These metabolites are essential for nutrient cycling,soil fertility,and crop productivity.There are many advantages to using microbial metabolites in agriculture,such as low dependence on chemical pesticides and fertilizers,increased crop productivity,and improved crop health.The challenges of production,formulation,consistency,and regulatory framework must be resolved before the microbial metabolites can be widely accepted.The future of agriculture will be shaped by advancements in microbial metabolite research,integrated with cutting-edge agricultural technologies and supported by aligned administrative policies.In summary,it is well established that microbial metabolites possess the capacity that significantly transform agriculture.Integrating the inherent potential of natural solutions can help create more sustainable and resilient agricultural systems that can protect food security,promote environmental sustainability,and ensure the future of future generations.