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The potential of metabolites released by Bacillus subtilis CH21 for the effective control of the harmful cyanobacterium Raphidiopsis raciborskii 认领 引用
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作者 Wei Zhang Xu Zeng +4 位作者 Jiayi Dong Yibin Su Erik Jeppesen Liqing Wang Sicheng Yin 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2026年第6期572-580,共9页
Microorganism-based biocontrol strategies for harmful cyanobacterial blooms have gained increasing attention due to their ecological compatibility and species-specific targeting.Despite this,research on algicidal bact... Microorganism-based biocontrol strategies for harmful cyanobacterial blooms have gained increasing attention due to their ecological compatibility and species-specific targeting.Despite this,research on algicidal bacteria effectiveness against the invasive cyanobacterium Raphidiopsis raciborskii remains limited.Research has been conducted on the Bacillus subtilis strain CH21,which was isolated from Chaohu Lake in China.exhibiting potent indirect anticyanobacterial activity against R.raciborskii.Within 48 h of exposure,the cell-free filtrate of strain CH21 achieved 96.2%removal efficiency of R.raciborskii cells.Physiological and morphological analyses revealed severe disruption of the photosynthetic apparatus(Fv/Fm and Yield values reduced to zero within 16 h),oxidative stress(elevation in malondialdehyde content and reactive oxygen species,reduction in superoxide dismutase and catalase activity),and cellular damage,including pseudovacuole collapse,the degradation of polyphosphate granules and cytoplasmic leakage.Metabolomic profiling identified surfactin-class lipopeptides as putative bioactive agents,which demonstrated exceptional stability across a wide thermal(25–121°C)and pH(3–13)range.These findings highlight B.subtilis CH21 as a promising candidate for targeted biocontrol of R.raciborskii blooms,supporting the development of environmentally mitigation technologies to be used in cases where it is difficult to control R.raciborskii by external nutrient loading reduction or if the response to such loading reductions are slow. 展开更多
关键词 Harmful cyanobacterial blooms Algicidal bacteria Raphidiopsis raciborskii Bacillus subtilis
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Overview of the distribution and adaptation of a bloom-forming cyanobacterium Raphidiopsis raciborskii:integrating genomics,toxicity,and ecophysiology 认领 引用 被引量:10
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作者 Zhongxing WU Songqi YANG Junqiong SHI 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2022年第5期1774-1791,共18页
Raphidiopsis raciborskii is a notorious bloom-forming and filamentous cyanobacterium that has been extensively investigated into its toxicity,phylogeny,and spreading potential.Studies have demonstrated that this speci... Raphidiopsis raciborskii is a notorious bloom-forming and filamentous cyanobacterium that has been extensively investigated into its toxicity,phylogeny,and spreading potential.Studies have demonstrated that this species has spanned different climates from tropical zones to temperate regions,suggesting that R.raciborskii is becoming a cosmopolitan species in freshwater systems around the world.In fact,it has been proposed that several characteristics of R.raciborskii may explain its spread and dominance.In particular,R.raciborskii is known to display a high extent of physiological plasticity regarding nutrients,light regimes,and te mperature s.Moreover,this species illustrates different ecotype s with distinct environmental requirements.Here,we present an overview of R.raciborskii’s global distribution and adaptation strategy based on the recent findings from genome variance,toxicity,and ecophysiology.The expansion of its geographical distribution can be linked to its genome,toxicity,and ecophysiology.The variable genes are mainly associated with the stress response,phage defense,DNA repair,cell cycle control,and membrane transport,illustrating the species’adaptability in response to changing environments.In fact,the species shows rapid adaptability to low and/or variable nutrient availability,especially changing phosphorus availability.Moreover,the variabilities of strains within the population extend their flexibility to adapt and acclimate to ambient environment.In addition,cylindrospermopsins(CYN)appear to have a potential biological role in facilitating theirs dominance or bloom.These strategies of R.raciborskii make it a challenge to manage in a fre shwater system,reflecting the management of its bloom from further evidence of the complex ecophysiology,toxicity,and genome of this species. 展开更多
关键词 distribution ecophysiology genome variance Raphidiopsis raciborskii toxicity
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Competitiveness of alga Microcystis aeruginosa co-cultivated with cyanobacterium Raphidiopsis raciborskii confirms its dominating position 认领 引用 被引量:2
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作者 Zengling MA Xiaoqiao ZHANG +7 位作者 Renhui LI Min WANG Wenli QIN He ZHANG Gang LI Henguo YU Chuanjun DAI Min ZHAO 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2022年第5期1804-1818,共15页
Microcystis aeruginosa has always been regarded as the main culprit of cyanobacterial blooms in freshwater.However,in recent years,Raphidiopsis raciborskii has gradually replaced M.aeruginosa as the culprit of cyanoba... Microcystis aeruginosa has always been regarded as the main culprit of cyanobacterial blooms in freshwater.However,in recent years,Raphidiopsis raciborskii has gradually replaced M.aeruginosa as the culprit of cyanobacterial blooms in some tropical and subtropical shallow lakes.To reveal which one plays a more dominant role,interactions between cylindrospermospin(CYN)-producing R.raciborskii and microcystins(MCs)-producing or non-MCs-producing M.aeruginosa strains were studied using bialgal cultures at different initial ratios of biomasses of the two species at 25℃.During the co-cultivation,the M.aeruginosa strains inhibited the growth and heterocyst formation of R.raciborskii filaments,and thus occupied a dominant position during the co-cultivation regardless of the initial biomass ratios in the cultures.In addition,the MCs-producing M aeruginosa strain contributed to a higher portion of the total biomass and exerted a stronger inhibitory effect on R.raciborskii compared with the non-MCs-producing strain.However,the growth of both MCs-producing and non-MCs-producing M.aeruginosa strains was stimulated by R.raciborskii in the co-cultures compared with M.aeruginosa monoculture,indicating that M.aeruginosa could outcompete R.raciborskii if given enough time,enabling it to develop into the dominant species even in very low initial concentration.To our best knowledge,this is the first report on the loss of heterocyst formation by a species of cyanobacteria that resulted from interactions between two different species of cyanobacteria.These findings indicate that it is difficult for R.raciborskii to replace the dominant position of M.aeruginosa under the experimental environmental condition,and the allelopathic effects of M.aeruginosa on R.raciborskii could significantly contribute to the success of M.aeruginosa. 展开更多
关键词 competition growth heterocyst Microcystis aeruginosa morphology Raphidiopsis raciborskii
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Physiological and molecular responses of invasive cyanobacterium Raphidiopsis raciborskii to ambient phosphorus deficiency 认领 引用 被引量:1
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作者 Junqiong SHI Shuhan HE +3 位作者 Lu ZHAO Lulu JI Songqi YANG Zhongxing WU 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2022年第5期1792-1803,共12页
Raphidiopsis raciborskii can cause harmful cyanobacterial blooms when concentrations of environme ntal pho sphorus(P)are very low,thus the physiological and molecular mechanisms involved in the acclimation to P need t... Raphidiopsis raciborskii can cause harmful cyanobacterial blooms when concentrations of environme ntal pho sphorus(P)are very low,thus the physiological and molecular mechanisms involved in the acclimation to P need to be characterized better.The growth,chlorophyll fluorescence,alkaline phosphatase,and expression of genes directly involved in P assimilation were compared in the R.raciborskii FACHB1496 strain grown with and without inorganic P.The specific growth rate(μ),Chl a,and six fluorescence parameters(minimal fluorescence(F),maximal fluorescence(F),maximal variable fluorescence(F),electron transport flux(further than QA)per RC(ET/RC),quantum yield of the electron transport in PSⅡ(?),and the probability that an electron from a trapped exciton is moved into the electron transport chain beyond Q~-(Ψ))markedly decreased in R.raciborskii in response to experimental P-deficiency.In contrast,the relative variable fluorescence at the J-step(V),trapped energy flux(leading to QA reduction)per RC(TR/RC),and alkaline phosphatase activity significantly increased.In addition,gene expressions involved in the alkaline phosphatase(phoAl and phoA2),high-affinity inorganic P transporter(pstSl),phosphonate transporter and metabolism(phnD and phnM),and nucleotidase(nucH)were significantly upregulated under P deficiency.However,physiological and molecular responses were resumed rapidly after P re-supplementation following P-deficient conditions.Our results highlight that R.raciborskii can perform coordinated and complex cellular and physiological responses to cope with P deficiency,reflecting R.raciborskii’s multi-faceted machinery to respond to environmental P fluctuations. 展开更多
关键词 phosphorus deficiency Raphidiopsis raciborskii gene expression chlorophyll fluorescence alkaline phosphatase
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The morphological and molecular detection for the presence of toxic Cylindrospermopsis(Nostocales, Cyanobacteria) in Beijing city, China 认领 引用 被引量:6
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作者 XIE Jinlin YU Gongliang +2 位作者 XU Xudong LI Shouchun LI Renhui 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2018年第2期263-272,共10页
Cylindrospermopsis raciborskii and its highly similar relatives Raphidiopsis species have been recognized as globally invasive and expansive filamentous cyanobacteria causing water blooms.Reports on C.raciborsMi/Raphi... Cylindrospermopsis raciborskii and its highly similar relatives Raphidiopsis species have been recognized as globally invasive and expansive filamentous cyanobacteria causing water blooms.Reports on C.raciborsMi/Raphidiopsis species and their harmful metabolites such as hepatotoxic cylindrospermopsins(CYNs)in Chinese waters have been increasing,but mostly restricted to the southern regions of China.To further explore the existence and distribution of C.raciborskii in China,six water samples from Beijing city were morphologically and molecularly examined.Five samples of the six were shown to have Cylindrospermopsis filaments with straight and spiral morphotypes.PCR detection targeting on Cylindrospermopsis/Raphidiopsis specific 16S rRNA gene region also showed the positive amplification,and such amplifications were confirmed by sequencing and phylogenetic analysis.As well,three of the five Cylindrospermopsis containing samples were shown to have cyrJ--a gene of CYN synthesis gene duster.The results represented the presence of toxic Cylindrospermopsis at the most northern line in China so far,indicating rapid expansion of this harmful invasive cyanobacterium.It is strongly suggested that the monitoring on C.raciborskii/Raphidiopsis species and their production of cylindrospermopsin should be emphasized in Beijing and even more northem parts of China. 展开更多
关键词 Beijing Cyanobacteria Cylindrospermopsin Cylindrospermopsis raciborskii Raphidiopsis
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