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Rapid testing methods for food contaminants and toxicants 认领 引用 被引量：11
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作者 Jiachi Chiou Arthur Ho Hon Leung +1 位作者 Hang Wai Lee Wing-tak Wong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2015年第11期2243-2264,共22页
Food safety is one of the major concerns in every country regardless of the economic and social development. The frequent occurrence of food scandals in the world has led the Chinese government to implement several st... Food safety is one of the major concerns in every country regardless of the economic and social development. The frequent occurrence of food scandals in the world has led the Chinese government to implement several strategies to fortify the food supply system to a high food safety standard. This relies heavily on laboratory testing services but conventional methods for detection of food contaminants and toxicants are limited by sophisticated sample preparation procedures, long analysis time, large instruments and professional personnel to meet the increasing demands. In this review, we have incorporated most of the current and potential rapid detection methods for many notorious food contaminants and toxicants including microbial agents, toxic ions, pesticides, veterinary drugs and preservatives, as well as detection of genetically modified food genes and adulterated edible oil. Development of rapid, accurate, easy-to-use and affordable testing methods could urge food handlers and the public to actively screen for food contaminants and toxicants instead of passively relying on monitoring by the government examination facility. This review also provides several recommendations including how to encourage the public to engage in the food safety management system and provide optimal education and financial assistance that may improve the current Chinese food safety control system. 展开更多
关键词 rapid testing food contaminants and toxins microbial agents pesticides veterinary drugs preservatives formaldehyde nitrite nitrate test strips
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Seroprevalence, Importance of Rapid Testing and Factors Associated with HBsAg Carriage in Pregnancy in Three Referral Maternity Hospitals in Mono (Benin), in 2020 认领 引用
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作者 Aboudou Raïmi Kpossou Djima Patrice Dangbemey +3 位作者 Véronique Mèdessè Tognifodé Barnard Acakpo Jean Sehonou Josiane Angeline Tonato Bagnan 《Open Journal of Gastroenterology》 CAS 2023年第9期279-298,共20页
Introduction Difficulties in accessing the screening test for viral hepatitis B in maternity wards could be a factor in underestimating the prevalence of anti-HBV antibodies. The rapid diagnostic orientation test (RDO... Introduction Difficulties in accessing the screening test for viral hepatitis B in maternity wards could be a factor in underestimating the prevalence of anti-HBV antibodies. The rapid diagnostic orientation test (RDOT) could improve seroprevalence and obstetrical prognosis. The aim of this study was to use the rapid diagnostic orientation test (RDOT) to determine the seroprevalence of HBs antigen carriage in pregnant women while highlighting the associated factors. Methods This was a cross-sectional and descriptive study that took place from October to December 2020 (three months) in three (03) reference maternities in western Benin (Mono). Consenting pregnant women received in prenatal consultation had been screened by RDOT. Positive cases were confirmed by ELISA test. The follow-up of these cases made it possible to establish the obstetrical prognosis. Results Of 201 women studied, 11 (5.5%) were positive for RDOT HBV and confirmed by the ELISA test. The factors associated with HBsAg carriage during pregnancy were multiple sexual partnerships (p = 0.01), female circumcision (p = 0.0001), and ignorance of prior HBV serological status (p = 0.0001). No influence of hepatitis B on pregnancy was noted. Conclusion The seroprevalence of hepatitis B in pregnancy was intermediate in the reference maternities of western Benin. The associated factors were multiple sexual partnerships, female circumcision and unawareness of prior HBV status. Free RDOT in maternity wards would improve early detection and management of viral hepatitis B in pregnancy. 展开更多
关键词 Hepatitis B Seroprevalence Associated Factors Rapid Test Benin
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Rapid Measurement of Elastic Modulus for Shaped Ceramic Tiles Based on Impulse Excitation Technique 认领 引用
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作者 SONG Yanyan FENG Zhiyuan +2 位作者 WANG Han WU Gang FAN Muxu 《China's Refractories》 CAS 2026年第1期12-16,共5页
This study addresses the challenge of directly determining the elastic modulus of complex shaped ceramic products—such as gas turbine combustor tiles—using conventional standardized methods,which are limited by spec... This study addresses the challenge of directly determining the elastic modulus of complex shaped ceramic products—such as gas turbine combustor tiles—using conventional standardized methods,which are limited by specimen geometry.A rapid,non-destructive testing method based on the impulse excitation technique(IET)and a shape factor coefficient was proposed.Three types of shaped ceramic tiles were selected.The elastic modulus of standard rectangular specimens obtained by destructive sampling was used as the reference value,and the shape factor coefficient for each tile type was calibrated by combining the mass and fundamental frequency of the whole tile.Using this coefficient,the elastic modulus of whole tiles was calculated solely from non-destructively measured mass and frequency.The results show that the deviation between the elastic modulus derived from the proposed method and that from destructive testing is less than 5%,confirming the accuracy and reliability of the approach.The method overcomes the shape restrictions inherent in traditional testing,offering a fast,non-destructive solution suitable for onsite quality assessment and process control during the production of shaped ceramic components. 展开更多
关键词 elastic modulus shape factor coefficient rapid testing shaped ceramic tiles
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Beads-on-a-Tip testing for ultrasensitive antigen detection across a large dynamic range 认领 引用
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作者 Ziwei Wu Yangjian Cai +4 位作者 Yitong Zhao Mahnaz Maddahfar Mohammad Sadraeian Dayong Jin Jiajia Zhou 《Smart Molecules》 CSCD 2026年第1期165-176,共12页
Lateral flow immunoassays(LFIAs)are low-cost,rapid,and easy to use for pointof-care testing(POCT),but the majority of the available LFIA tests are indicative,rather than quantitative,and their sensitivity in antigen t... Lateral flow immunoassays(LFIAs)are low-cost,rapid,and easy to use for pointof-care testing(POCT),but the majority of the available LFIA tests are indicative,rather than quantitative,and their sensitivity in antigen tests are usually limited at the nanogram range,which is primarily due to the passive capillary fluidics through nitrocellulose membranes,often associated with non-specific bindings and high background noise.To overcome this challenge,we report a Beads-on-a-Tip design by replacing nitrocellulose membranes with a pipette tip loaded with magnetic beads.The beads are pre-conjugated with capture antibodies that support a typical sandwich immunoassay.This design enriches the low-abundant antigen proteins and allows an active washing process to significantly reduce non-specific bindings.To further improve the detection sensitivity,we employed upconversion nanoparticles(UCNPs)as luminescent reporters and SARS-CoV-2 spike(S)antigen as a model analyte to benchmark the performance of this design against our previously reported methods.We found that the key to enhance the immunocomplex formation and signal-to-noise ratio lay in optimizing incubation time and the UCNP-to-bead ratio.We therefore successfully demonstrated that the new method can achieve a very large dynamic range from 500 fg/mL to 10μg/mL,across over 7 digits,and a limit of detection of 706 fg/mL,nearly another order of magnitude lower than the best reported LFIA using UCNPs in COVID-19 spike antigen detection.Our system offers a promising solution for ultra-sensitive and quantitative POCT diagnostics. 展开更多
关键词 Beads-on-a-Tip COVID-19 rapid testing ultrasenstive assay upconversion nanoparticles
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Secondary analysis of malaria rapid diagnostic tests from rounds 5–8 of WHO product testing with a focus on false-negative results 认领 引用
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作者 Biao Xu Bo Tu +4 位作者 Fang Chu Mohamed Jalloh Jin-Song Mu Jun-Jie Zheng Wei-Wei Chen 《Military Medical Research》 SCIE CAS CSCD 2022年第3期379-381,共3页
Despite the widespread use of malaria rapid diagnostic test(RDT)in clinical practice,there are a lot of challenges.We conducted a secondary analysis of 129 malaria RDT data from rounds 5–8 of the World Health Organiz... Despite the widespread use of malaria rapid diagnostic test(RDT)in clinical practice,there are a lot of challenges.We conducted a secondary analysis of 129 malaria RDT data from rounds 5–8 of the World Health Organization(WHO)product testing summary and discuss the causes of false-negative(FN)results with a focus on low parasite density,improper RDT storage,operation and interpretation,and Plasmodium falciparum with a pfhrp2/3 gene deletion.The results demonstrated that the malaria RDTs currently commercially available might cause FN results in practice. 展开更多
关键词 Malaria Rapid diagnostic tests False-negative results WHO product testing
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Identification of coal characteristics by near-infrared spectroscopy:Machine learning predictions and experimental validations 认领 引用
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作者 AN Haiquan LIU Zhen +1 位作者 LI Ye PENG Baozi 《燃料化学学报（中英文）》 EI CAS CSCD 北大核心 2026年第5期201-212,共12页
In this study,near-infrared spectroscopy was used along with various machine learning algorithms to develop a fast and reliable framework for identifying coal components,which significantly improved the accuracy and e... In this study,near-infrared spectroscopy was used along with various machine learning algorithms to develop a fast and reliable framework for identifying coal components,which significantly improved the accuracy and efficiency of predictions.Support vector machine(SVM),random forest(RF),temporal convolutional network(TCN),one-dimensional convolutional neural network(1D CNN),and gated recurrent unit(GRU)algorithms were used for regression modeling of coal properties.Among the various models considered herein,the TCN model was shown to provide the lowest Mean Absolute Error(MAE)value,which was 0.505.In addition,the RF model demonstrated the lowest Root Mean Square Error(RMSE)value of 0.618,indicating its robustness in prediction on the test set.The TCN model,on the other hand,had the lowest Coefficient of Variation(CV)value of 0.042,which demonstrated its good generalization ability and stability.Furthermore,the developed single-output models revealed that different models have different predictive effects on coal components.For example,it was found that the TCN model is optimal for predicting ash content,while the RF model is the most accurate model for predicting fixed carbon and low calorific value.Taking into account the quantity of model parameters,computation time,and accuracy analysis,the results showed that the RF,1D CNN and TCN models are more efficient.Finally,the influence of key characteristic peaks on prediction results was revealed through SHAP value analysis.The results showed a relationship between absorption intensity and composition of organic and inorganic values in coal to near-infrared light,suggesting important characteristic spectral ranges for ash,moisture,and fixed carbon.This research not only provides a new method for rapid detection in the coal industry,but also serves as a reference for component analysis in other fields. 展开更多
关键词 machine learning coal characteristics NIR rapid testing
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Human immunodeficiency virus recency testing coverage and partner-notification-services among people-living with human immunodeficiency virus in low- and middle-income countries 认领 引用
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作者 Ibrahim Ahmed El-Imam Timothy Antipas Peter +7 位作者 Hassan Fredrick Fussi Zuhura Mbwana Ally Hafidha Mhando Bakari Mariam Salim Mbwana Upendo Kayeke Chenya Beatrice Kelvin Mpimo Haji Mbwana Ally Habib Omari Ramadhani 《World Journal of Virology》 2025年第4期112-125,共14页
BACKGROUND Human immunodeficiency virus(HIV)recency testing provides data that can be used to monitor the trend of new HIV infections.The effectiveness of using people identified with recent infection to identify part... BACKGROUND Human immunodeficiency virus(HIV)recency testing provides data that can be used to monitor the trend of new HIV infections.The effectiveness of using people identified with recent infection to identify partners with new HIV infection through partner notification services(PNS)is not well documented.AIM To determine the pooled prevalence of recency testing coverage,recent infection,reclassification(recent to longterm infection)and PNS cascade among newly diagnosed people living with HIV.METHODS PubMed,Cochrane Library and Embase were searched for articles published between January 2018 and November 2024.Studies were included if they reported recency coverage and/or PNS among people newly diagnosed with HIV and used recent infection testing algorithm(RITA).Recency coverage was defined as proportion of people tested using rapid testing for recent infection(RTRI)among those newly diagnosed with HIV.RITA further classifies RTRI results using viral load results(≥1000 copies/mL vs<1000 copies/mL)to confirm recency status.For studies with PNS,we evaluated the cascade:Number of partners elicited,successfully contacted,eligible for HIV testing,tested and HIV diagnosis.PNS effectiveness was measured by proportion of new HIV diagnoses from tested partners.Using random effects models,we computed the pooled estimate of recency outcomes and 95%confidence intervals(CIs).RESULTS Twenty-five studies from 17-low-and middle-income countries were included.Of 276315 newly diagnosed people living with HIV,79864 underwent RTRI with an overall pooled recency coverage of 87%(95%CI:67-96).The pooled prevalence of RTRI and RITA recency were 12%(95%CI:9-16)and 7%(95%CI:4-10),respectively.Pooled prevalence of RTRI reclassification was 34%(95%CI:22-49).Of the recent cases who agreed to PNS,253 partners were elicited with an estimated elicitation ratio of 1:1.6.Among partners elicited,99%were successfully contacted,75%were eligible for testing,68%tested for HIV,and 15%were diagnosed with HIV.CONCLUSION High recency testing coverage among newly diagnosed individuals demonstrates the feasibility of monitoring new HIV infections in LMIC.While PNS yielded moderate HIV diagnoses,its targeted approach remains a critical strategy for identifying undiagnosed cases. 展开更多
关键词 Human immunodeficiency virus recency testing coverage Rapid test for recent infection Recent infection testing algorithm Partner notification services Misclassification Recent infection surveillance Low-and mid-income countries
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Dual specimens increase the diagnostic accuracy and reduce the reaction duration of rapid urease test 认领 引用 被引量：14
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作者 Wen-Hung Hsu Sophie SW Wang +10 位作者 Chao-Hung Kuo Chiao-Yun Chen Ching-Wen Chang Huang-Ming Hu Jaw-Yuan Wang Yuan-Chieh Yang Yu-Chun Lin Wen-Ming Wang Deng-Chyang Wu Ming-Tsang Wu Fu-Chen Kuo 《World Journal of Gastroenterology》 SCIE CAS 2010年第23期2926-2930,共5页
AIM:To evaluate the influence of multiple samplings during esophagogastr oduodenoscopy(EGD)on the accuracy of the rapid urease test,and the validity of newly developed rapid urease tests,HelicotecUT plus test and Heli... AIM:To evaluate the influence of multiple samplings during esophagogastr oduodenoscopy(EGD)on the accuracy of the rapid urease test,and the validity of newly developed rapid urease tests,HelicotecUT plus test and HelicotecUT test,CLO test and ProntoDry test.METHODS:A total of 355 patients undergoing EGD for dyspepsia were included.Their Helicobacter pylori(H.pylori)treatment status was either nave or eradicated.Six biopsy specimens from antrum and gastric body,respectively,were obtained during EGD.Single antral specimens and dual(antrum+body)specimens were compared.Infection status of H.pylori was evaluated by three different tests:culture,histology,and four different commercially available rapid urease tests(RUTs)-including the newly developed HelicotecUT plus test and HelicotecUT test,and established CLO test and ProntoDry test.H.pylori status was defined as positive when the culture was positive or if there were concordant positive results among histology,CLO test and ProntoDry test.RESULTS:When dual specimens were applied,sensitivity was enhanced and RUT reaction time was signif icantly reduced,regardless of their treatment status.Thirty minutes were enough to achieve an agreeable positive rate in all the RUTs.Both newly developed RUTs showed comparable sensitivity,specif icity and accuracy to the established RUTs,regardless of patient treatment status,RUT reaction duration,and EGD biopsy sites.CONCLUSION:Combination of antrum and body biopsy specimens greatly enhances the sensitivity of rapid urease test and reduces the reaction duration to 30 min. 展开更多
关键词 Rapid urease test Helicobacter pylori eradication HelicotecUT plus test HelicotecUT test Esophagogastroduodenoscopy
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Establishment and Preliminary Application of a Rapid Fluorescent Focus Inhibition Test (RFFIT) for Rabies Virus 认领 引用 被引量：13
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作者 Pengcheng Yu Xinjun Lv +2 位作者 Xinxin Shen Qing Tang Guodong Liang 《Virologica Sinica》 CAS CSCD 2013年第4期223-227,共5页
The World Health Organization (WHO) standard assay for determining levels of the rabies virus neutralization antibody (RVNA) is the rapid fluorescent focus inhibition test (RFFIT), which is used to evaluate the immuni... The World Health Organization (WHO) standard assay for determining levels of the rabies virus neutralization antibody (RVNA) is the rapid fluorescent focus inhibition test (RFFIT), which is used to evaluate the immunity effect after vaccination against rabies. For RFFIT, CVS-11 was used as the challenge virus, BSR cells as the adapted cells, and WHO rabies immunoglobulin (WHO STD) as the reference serum in this study. With reference to WHO and Pasteur RFFIT procedures, a micro-RFFIT procedure adapted to our laboratory was produced, and its specificity and reproducibility were tested. We tested levels of RVNA in human serum samples after immunization with different human rabies vaccines (domestic purified Vero cell rabies vaccine (PVRV) and imported purified chick embryo cell vaccine (PCECV)) using different regimens (Zagreb regimen and Essen regimen). We analyzed the levels of RVNA, and compared the immune efficacy of domestic PVRV and imported PCECV using different immunization regimens. The results showed that the immune efficacy of domestic PVRV using the Zagreb regimen was as good as that of the imported PCECV, but virus antibodies were generated more rapidly with the Zagreb regimen than with the Essen regimen. The RFFIT procedure established in our laboratory will enhance the comprehensive detection ability of institutions involved in rabies surveillance in China. 展开更多
关键词 Rapid fluorescent focus inhibition test （RFFIT） RVNA Rabies Vaccination regimen
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Seroprevalence and clinical profile of dengue infection among acute febrile patients at a rural tertiary care hospital 认领 引用
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作者 Shree V Dhotre Mangala P Ghatole +2 位作者 Pradnya S Dhotre Virendra A Kashetty Basavraj S Nagoba 《World Journal of Virology》 2026年第1期175-181,共7页
BACKGROUND Dengue fever remains a major cause of acute febrile illness in India,particularly in rural areas where diagnostic facilities are limited.Early detection using point-ofcare tests helps guide timely managemen... BACKGROUND Dengue fever remains a major cause of acute febrile illness in India,particularly in rural areas where diagnostic facilities are limited.Early detection using point-ofcare tests helps guide timely management and reduce unnecessary antibiotic use.AIM To determine the seroprevalence and clinical profile of dengue infection among acute febrile patients attending a rural tertiary care hospital in Maharashtra.METHODS A prospective cross-sectional study was conducted at Ashwini Rural Medical College Hospital and Research Centre,Solapur from May to October 2025.A total of 320 consecutive patients presenting with acute fever of≤7 days duration prior to hospital presentation were enrolled.Patients of all age groups were included.Serum samples were tested for dengue NS1 antigen and anti-dengue IgM and IgG antibodies using a commercial rapid test(Manufacturer:Meril diagnostics;Meriscreen dengue NS1 antigen and IgM+IgG antibodies).A 10%subset(n=32)was tested by enzyme-linked immunosorbent assay(ELISA)for quality assurance.Patients were classified as recent dengue(NS1+and/or IgM+),probable secondary infection(IgM+IgG+),past exposure(IgG+IgM-),or negative(NS1-IgM-).Clinical data and outcomes were recorded.Statistical analysis includedχ2,Mann-Whitney U,and logistic regression(α=0.05),adjusting for age,sex,platelet count,total leukocyte count,and presence of rash.RESULTS Overall,28%(90/320)had evidence of recent dengue,while 35%(112/320)showed IgG positivity,and 10%(32/320)had probable secondary infection.Median platelet count was significantly lower in the recent dengue group[78000/mm3;interquartile range(IQR):55000-110000]compared with non-dengue patients(210000/mm3;IQR:160000-240000;P<0.001).The most common symptoms were headache(68%),myalgia(61%),and retroorbital pain(44%).Antibiotic use prior to testing was 60%;among those diagnosed with dengue,a relative 25%reduction in antibiotic prescriptions was observed following laboratory confirmation.Quality assurance analysis showed rapid test sensitivity of 92%,specificity of 95%,and Cohen’s kappa of 0.85 vs ELISA.Logistic regression identified thrombocytopenia(<100000/mm3)[adjust odds ratio(aOR)5.2;95%CI:2.4-10.9]and rash(aOR 3.6;95%CI:1.8-7.0)as independent predictors of dengue positivity.CONCLUSION A substantial proportion of acute febrile cases in this rural setting were due to dengue,highlighting the utility of NS1/IgM rapid tests for early diagnosis where molecular facilities are unavailable.Integrating rapid dengue testing into routine fever evaluation can improve case detection and rationalize antibiotic use. 展开更多
关键词 Dengue fever Seroprevalence NS1 antigen IgM/IgG antibodies Rural hospital Rapid test Platelet count
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Comparison of RFFIT Tests with Different Standard Sera and Testing Procedures 认领 引用 被引量：7
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作者 Peng-cheng Yu Akira Noguchi +3 位作者 Satoshi Inoue Qing Tang Simon Rayner Guo-dong Liang 《Virologica Sinica》 CAS CSCD 2012年第3期187-193,共7页
The World Health Organization （WHO） standard assay for determining antibody level is the rapid fluorescent focus inhibition test （RFFIT） and is used to determine the degree of immunity after vaccination against ra... The World Health Organization （WHO） standard assay for determining antibody level is the rapid fluorescent focus inhibition test （RFFIT） and is used to determine the degree of immunity after vaccination against rabies. To compare the difference in RFFIT results between the laboratories of The National Institute of Infectious Disease in Japan （NIID） and the Chinese Centre for Disease Control （CCDC） as well the influence of the choice of standard serum （STD） for the detection, the two laboratories detection methods were simultaneously manipulated by RFFIT. The reference serums used in NIID and the WHO standard serum used in CCDC were compared in the same RFFIT detection to determine the titer of four sera samples C1, Sl, S2 and S4 in parallel, and the titers of the detected sera samples were calculated using the standard formula for neutralizing antibody titer. No significant difference was found in RFFIT methods from the two laboratories and the RFFIT testing procedures of the two laboratories have good consistency. However, different titers were obtained with the tentative internal standard serum （TI-STD） produced by adjusting to 2.0 IU of WHO standard serum in NIID and the WHO STD. The titer determined with the TI-STD was higher than that determined with WHO STD, This difference appears to be significant and requires further investigation 展开更多
关键词 Rapid fluorescent focus inhibition test （RFFIT） Standard serum Neutralizing antibody Titer
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Intraprocedural gastric juice analysis as compared to rapid urease test for real-time detection of Helicobacter pylori 认领 引用 被引量：3
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作者 Riccardo Vasapolli Florent Ailloud +7 位作者 Sebastian Suerbaum Jens Neumann Nadine Koch Lukas Macke Jörg Schirra Julia Mayerle Peter Malfertheiner Christian Schulz 《World Journal of Gastroenterology》 SCIE CAS 2023年第10期1638-1647,共10页
BACKGROUND Endofaster is an innovative technology that can be combined with upper gastrointestinal endoscopy(UGE)to perform gastric juice analysis and real-time detection of Helicobacter pylori(H.pylori).AIM To assess... BACKGROUND Endofaster is an innovative technology that can be combined with upper gastrointestinal endoscopy(UGE)to perform gastric juice analysis and real-time detection of Helicobacter pylori(H.pylori).AIM To assess the diagnostic performance of this technology and its impact on the management of H.pylori in the real-life clinical setting.METHODS Patients undergoing routine UGE were prospectively recruited.Biopsies were taken to assess gastric histology according to the updated Sydney system and for rapid urease test(RUT).Gastric juice sampling and analysis was performed using the Endofaster,and the diagnosis of H.pylori was based on real-time ammonium measurements.Histological detection of H.pylori served as the diagnostic gold standard for comparing Endofaster-based H.pylori diagnosis with RUT-based H.pylori detection.RESULTS A total of 198 patients were prospectively enrolled in an H.pylori diagnostic study by Endofasterbased gastric juice analysis(EGJA)during the UGE.Biopsies for RUT and histological assessment were performed on 161 patients(82 men and 79 women,mean age 54.8±19.2 years).H.pylori infection was detected by histology in 47(29.2%)patients.Overall,the sensitivity,specificity,accuracy,positive predictive value,and negative predictive value(NPV)for H.pylori diagnosis by EGJA were 91.5%,93.0%,92.6%,84.3%,and 96.4%,respectively.In patients on treatment with proton pump inhibitors,diagnostic sensitivity was reduced by 27.3%,while specificity and NPV were unaffected.EGJA and RUT were comparable in diagnostic performance and highly concordant in H.pylori detection(κ-value=0.85).CONCLUSION Endofaster allows for rapid and highly accurate detection of H.pylori during gastroscopy.This may guide taking additional biopsies for antibiotic susceptibility testing during the same procedure and then selecting an individually tailored eradication regimen. 展开更多
关键词 Helicobacter pylori diagnostic Chronic gastritis Gastric juice Endofaster Rapid urease test Antimicrobial susceptibility testing
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Development of novel-nanobody-based lateral-flow immunochromatographic strip test for rapid detection of recombinant human interferon a2b 认领 引用 被引量：2
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作者 Xi Qin Maoqin Duan +13 位作者 Dening Pei Jian Lin Lan Wang Peng Zhou Wenrong Yao Ying Guo Xiang Li Lei Tao Youxue Ding Lan Liu Yong Zhou Chuncui Jia Chunming Rao Junzhi Wang 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2022年第2期308-316,共9页
Recombinant human interferon a2b(rhIFNa2b)is widely used as an antiviral therapy agent for the treatment of hepatitis B and hepatitis C.The current identification test for rhIFNa2b is complex.In this study,an anti-rhI... Recombinant human interferon a2b(rhIFNa2b)is widely used as an antiviral therapy agent for the treatment of hepatitis B and hepatitis C.The current identification test for rhIFNa2b is complex.In this study,an anti-rhIFNa2b nanobody was discovered and used for the development of a rapid lateral flow strip for the identification of rhIFNa2b.RhIFNa2b was used to immunize an alpaca,which established a phage nanobody library.After five steps of enrichment,the nanobody I22,which specifically bound rhIFNa2b,was isolated and inserted into the prokaryotic expression vector pET28a.After subsequent purification,the physicochemical properties of the nanobody were determined.A semiquantitative detection and rapid identification assay of rhIFNa2b was developed using this novel nanobody.To develop a rapid test,the nanobody I22 was coupled with a colloidal gold to produce lateral-flow test strips.The developed rhIFNa2b detection assay had a limit of detection of 1 mg/mL.The isolation of I22 and successful construction of a lateral-flow immunochromatographic test strip demonstrated the feasibility of performing ligand-binding assays on a lateral-flow test strip using recombinant protein products.The principle of this novel assay is generally applicable for the rapid testing of other commercial products,with a great potential for routine use in detecting counterfeit recombinant protein products. 展开更多
关键词 Recombinant human interferon a2b Nanobody Phage display Screening Rapid test
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Evaluation of a Direct Rapid Immunohistochemical Test (dRIT) for Rapid Diagnosis of Rabies in Animals and Humans 认领 引用 被引量：4
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作者 Shampur Narayan Madhusudana Sundaramurthy Subha +1 位作者 Ullas Thankappan Yajaman Belludi Ashwin 《Virologica Sinica》 CAS CSCD 2012年第5期299-302,共4页
Presently the gold standard diagnostic technique for rabies is the direct immunofluorescence assay （dFA） which is very expensive and requires a high level of expertise. There is a need for more economical and user f... Presently the gold standard diagnostic technique for rabies is the direct immunofluorescence assay （dFA） which is very expensive and requires a high level of expertise. There is a need for more economical and user friendly tests, particularly for use in developing countries. We have established one such test called the direct rapid immunohistochemical test （dRIT） for diagnosis of rabies using brain tissue. The test is based on capture of rabies nucleoprotein （N） antigen in brain smears using a cocktail of biotinylated monoclonal antibodies specific for the N protein and color development by streptavidin peroxidase-amino ethyl carbazole and counter staining with haematoxollin. The test was done in parallel with standard FAT dFA using 400 brain samples from different animals and humans. The rabies virus N protein appears under fight microscope as reddish brown particles against a light blue background. There was 100 % correlation between the results obtained by the two tests. Also, interpretation of results by dRIT was easier and only required a light microscope. To conclude, this newly developed dRIT technique promises to be a simple, cost effective diagnostic tool for rabies and will have applicability in field conditions prevalent in developing countries. 展开更多
关键词 Rabies, Post-mortem diagnosis Fluorescent antibody technique Immunohistochemistry Direct rapid immunohistochemical test（dRIT）
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Value of a new stick-type rapid urine test for the diagnosis of Helicobacter pylori infection in the Vietnamese population 认领 引用 被引量：4
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作者 Duc T Quach Toru Hiyama +5 位作者 Fumio Shimamoto Quang D Le Linh X Ho Nhu HT Vu Masaharu Yoshihara Naomi Uemura 《World Journal of Gastroenterology》 SCIE CAS 2014年第17期5087-5091,共5页
AIM: To assess the value of a new test for the diagnosis of Helicobacter pylori (H. pylori) infection, Rapirun&#x000ae;H. pylori Antibody Stick (Rapirun&#x000ae; Stick), in a Vietnamese population.
关键词 Helicobacter pylori, Urine test, Rapirun® Stick, Vietnamese, Rapid urease test
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Usefulness of the Japanese version of Rapid Dementia Screening Test for mild cognitive impairment in older patients with cardiovascular disease:a cross-sectional study 认领 引用 被引量：2
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作者 Takuji Adachi Yuki Tsunekawa +1 位作者 Akihito Matsuoka Daisuke Tanimura 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2021年第4期245-251,共7页
BACKGROUND Cognitive decline is common among older patients with cardiovascular disease(CVD) and can decrease their self-management abilities. However, the instruments for identifying mild cognitive impairment(MCI) ar... BACKGROUND Cognitive decline is common among older patients with cardiovascular disease(CVD) and can decrease their self-management abilities. However, the instruments for identifying mild cognitive impairment(MCI) are not always feasible in clinical practice. Therefore, this study evaluated whether MCI could be detected using the Japanese version of the Rapid Dementia Screening Test(RDST-J), which is a simple screening tool for identifying cognitive decline.METHODS This retrospective single-center study included patients who were ≥ 65 years old and hospitalized because of CVD.Patients with a pre-hospitalization diagnosis of dementia were excluded. Each patient's cognitive function had been measured at discharge using the RDST-J and the Japanese version of the Montreal Cognitive Assessment(Mo CA-J), which is a standard tool for MCI screening. The correlation between the two scores was evaluated using Spearman's rank correlation coefficient. Receiver operating characteristic(ROC) analysis was also to evaluate whether the RDST-J could identify MCI, which was defined as a Mo CA-J score of ≤ 25 points.RESULTS The study included 78 patients(mean age: 77.2 ± 8.9 years). The RDST-J and Mo CA-J scores were strongly correlated(r = 0.835, P < 0.001). The ROC analysis revealed that an RDST-J score of ≤ 9 points provided 75.4% sensitivity and 95.2% specificity for identifying MCI, with an area under the curve of 0.899(95% CI: 0.835-0.964). The same cut-off value was identified when excluding patients with a high probability of dementia(RDST-J score of ≤ 4 points).CONCLUSIONS The RDST-J may be a simple and effective tool for identifying MCI in older patients with CVD. 展开更多
关键词 CVD MCI Usefulness of the Japanese version of Rapid Dementia Screening Test for mild cognitive impairment in older patients with cardiovascular disease:a cross-sectional study
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Effect of gender on the reliability of COVID-19 rapid antigen test among elderly 认领 引用 被引量：2
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作者 Wassan Nori Wisam Akram 《World Journal of Clinical Cases》 SCIE 2022年第29期10820-10822,共3页
Defining con-founders that affect the reliability of diagnostic tests for coronavirus disease 2019 is vital to breaking the chain of infection.The elderly population is a higher risk group for the emerging virus.Howev... Defining con-founders that affect the reliability of diagnostic tests for coronavirus disease 2019 is vital to breaking the chain of infection.The elderly population is a higher risk group for the emerging virus.However,gender seems to exert a critical role in modifying the infection risk among women owing to hormonal changes.The menopause transition is an exceptional period for older women where the protective and immunomodulatory effects of the estrogen hormone are lost.Accordingly,attention should be given to postmenopausal women since they will have an increased risk compared to their pre-menopausal peers. 展开更多
关键词 COVID-19 Rapid antigen test Real-time reverse transcription-polymerase chain reaction Age Gender Postmenopausal women
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Evaluation of the use of rapid urease test:Pronto Dry to detect H pylori in patients with dyspepsia in several cities in Indonesia 认领 引用
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作者 AF Syam M Abdullah +5 位作者 AA Rani S Nurdjanah P Adi A Djumhana P Tarigan IDN Wibawa 《World Journal of Gastroenterology》 SCIE CAS 2006年第38期6216-6218,共3页
AIM： To evaluate Pronto Dry examination in patients with dyspepsia. METHODS： The study was conducted in patients with dyspepsia who underwent endoscopic examination in several endoscopic centers of several cities in... AIM： To evaluate Pronto Dry examination in patients with dyspepsia. METHODS： The study was conducted in patients with dyspepsia who underwent endoscopic examination in several endoscopic centers of several cities in Indonesia from January 2003 until April 2004. Biopsies for histopathologic examination were fixed with formalin and sent to Histopathologic Department to be analyzed and confirm the presence of Hpylori infection. If Hpylori was found positive, the density was calculated semi quantitatively. Histopathologic examination from gastric biopsy samples was interpreted based on the updated Sydney system classification. RESULTS： Of 550 patients, 309 （56%） were male and 241 （44%） were female with ages ranging from 15 to 82 years. Mean age was 44.98 ± 14.46 years. Mean age of male patients was 44.35 ± 13.85 years and mean age of female patients was 45.78± 15.19 years. Evaluation of endoscopic results showed gastric ulcer in 36 cases （6.5%） and duodenal ulcer in 20 cases （3.6%）. Normal endoscopic finding was found in 45 cases （8.2%） and minimal disorder of gastritis and duodenitis were found in 246 cases （44.7%）. One case of gastric cancer was identified. Of 56 cases which were positive based on the criteria used, 39 patients were positive with Pronto Dry and 17 patients were negative with Pronto Dry. Overall sensitivity and specificity of Pronto Dry were 69.7% and 95.7% respectively. Positive predictive value was 66.1% and negative predictive value was 96.4% and overall accurate rate was 92.9%. CONCLUSION： Pronto Dry seems promising as a diagnostic tool to detect Hpy/ori more rapidly and accurately. 展开更多
关键词 Rapid urease test Pronto Dry H pylori Dyspepsia
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Field Evaluation of Alternative Testing Strategies for the Detection of HIV Infection in Beijing 认领 引用
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作者 FA-XIN HEI YAN JIANG +6 位作者 WEI-DONG SUN QI-YUN ZHANG QIN ZHANG JING-RONG YE HAI-LIN LIU HONG-YAN LU AND XIONG HE 《Biomedical and Environmental Sciences》 SCIE CAS 2007年第4期265-268,共4页
To identify a cost-efficient altemative antibody testing strategy for screening and confirmation of HIV infection by rapid simple tests （RSTs） and enzyme-linked immunosorbent assays （ELISAs）. Methods Four RSTs （R... To identify a cost-efficient altemative antibody testing strategy for screening and confirmation of HIV infection by rapid simple tests （RSTs） and enzyme-linked immunosorbent assays （ELISAs）. Methods Four RSTs （RST1, RST2, RST3, and RST4 ） and five ELISAs （ELISA1, ELISA2, ELISA3, ELISA4, and ELISA5） were evaluated in two phases by using banked and serum specimens prospectively collected at regional hospitals and voluntary counseling and testing （VCT） centers in Beijing. A total of 200 banked serum specimens were included in the first phase, including 62 HIV-positive, 127 HIV-negative and 11 indeterminate specimens. All specimens were tested by four RSTs and five ELISAs respectively. The second phase involved prospective testing of 389 routine specimens, including 92 HIV-positive, 287 HIV-negative, and 10 indeterminate specimens. All the specimens were tested by two RSTs （RST2 and RST4） and three ELISAs （ELISA1, ELISA3, and ELISA4）, which were selected for their respective excellent sensitivity and/or specificity. Westem blot （WB） was used as a gold standard for confirming the reactivity of all the specimens. Results Sensitivity, specificity, and efficacy were calculated for each assay in two phases. In the first phase, four assays （ELISA4, RST2, RST3, and RST4） had a specificity of 100%. For the determination of efficacy, ELISA4, RST2, and RST4 were selected in the second phase. ELISA1 and ELISA3 which have a sensitivity of 95.9% and 93.2% respectively also entered this phase. In the second phase, all the five assays （ELISA1, ELISA3, ELISA4, RST2, and RST4） had a sensitivity and specifity of over 90%. ELISA1 had a sensitivity of 99% and ELISA4 a specificity of 99%. Conclusion The sensitivity ELISA1 and the specificit of ELISA4 are comparable to ELISA/WB standard strategy. Application of this alternative testing strategy provides a cost-effective method for determining HIV prevalence in Beijing. 展开更多
关键词 Human immunodeficiency virus type-l Altemative testing strategy Rapid simple test Enzyme-linkedimmunosorbent assay
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Rapid ultrasonic C-scan on image test for spot welding based interpolation 认领 引用
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作者 刘静 徐国成 +2 位作者 史延利 周广浩 谷晓鹏 《China Welding》 EI CAS 2014年第4期37-43,共7页
In this paper, ultrasonic C-scan test of spot welds for stainless steel has been studied. It is concluded that large scanning step length contributes to high testing efficiency, however, the low-resolution C-scan imag... In this paper, ultrasonic C-scan test of spot welds for stainless steel has been studied. It is concluded that large scanning step length contributes to high testing efficiency, however, the low-resolution C-scan image generated cannot be used to assess spot welding quality reliably. Based on bicubic image interpolation, the C-scan image in low resolution with the large step length 1 000 ~xm is subdivided and reconstructed. By this means, the C-scan image resolution is greatly enhanced and testing results obtained are satisfactory, realizing rapid assessment of spot welds. The results of rapid ultrasonic C-scan test fit the actual metallographic measured value well. Mean value of normal distribution of error statistics is O. 006 67, and the standard deviation is O. 087 11. Rapid ultrasonic C-scan test based on image interpolation is of high accuracy and excellent stability. 展开更多
关键词 rapid ultrasonic test C-scan test image interpolation spot welds nugget diameter
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