Rootstock plays an important role in commercial horticultural production by influencing scion growth,physiology,and fruit quality.However,information on the effects of different rootstocks on kiwifruit growth and frui...Rootstock plays an important role in commercial horticultural production by influencing scion growth,physiology,and fruit quality.However,information on the effects of different rootstocks on kiwifruit growth and fruit quality remains limited.In this study,Actinidia valvata was evaluated as an experimental rootstock and compared with the commonly used A.deliciosa rootstock to investigate their effects on photosynthetic characteristics,carbohydrate metabolism,and fruit quality of A.deliciosa cv.‘Guichang’.Leaf photosynthetic parameters,fruit quality attributes,carbohydrate contents,and the activities of enzymes related to sucrose and starch metabolism were investigated during fruit development and postharvest ripening.The results showed that vines grafted onto A.valvata exhibited higher photosynthetic performance at certain developmental stages.Favorable changes were also detected in several physiological indicators associated with starch and sugar accumulation.In addition,fruits from plants grafted onto A.valvata exhibited enhanced activities of key enzymes involved in sucrose and starch metabolism at specific sampling stages.However,for many measured variables and time points,no significant differences were detected between the two rootstocks.Overall,these results suggest that A.valvata has potential as an alternative rootstock for‘Guichang’kiwifruit,particularly with respect to carbohydrate metabolism,although its overall performance was broadly comparable to that of A.deliciosa.展开更多
In rootstock-scion combinations,the rootstock is the key factor determining a plant's salt tolerance,primarily through its root physiology and soil interaction capabilities.However,the interaction between rootstoc...In rootstock-scion combinations,the rootstock is the key factor determining a plant's salt tolerance,primarily through its root physiology and soil interaction capabilities.However,the interaction between rootstock and scion in influencing salt tolerance in apple trees remains underexplored.In this study,three one-year-old dwarf rootstocks—'Qingzhen-1','M9-T337',and'(Malus hupehensis Rehd,var.Pingyiensis,PYTC)'—were selected,with'Luli'used as the scion.The salt tolerance was compared in natural soil(1‰),and saline-alkali soil from Dongying(3‰,6‰,10‰).The results showed that salt stress inhibited plant growth and damaged root structure.Among the combinations,Luli/Qingzhen-1 exhibited superior growth performance,with reduced malondialdehyde content,relative conductivity,and mortality.Additionally,in the Luli/Qingzhen-1 combination,genes associated with antioxidant enzyme activity and stress signal transduction were significantly upregulated.In conclusion,Qingzhen-1 demonstrates greater salt tolerance than M9-T337 and PYTC,making it a promising rootstock for cultivating'Luli'scions in saline-alkali soils.展开更多
Grafting is a propagation method extensively utilized in cucurbits.However,the mechanisms underlying graft healing remain poorly understood.This study employed self-grafted watermelon plants to investigate how rootsto...Grafting is a propagation method extensively utilized in cucurbits.However,the mechanisms underlying graft healing remain poorly understood.This study employed self-grafted watermelon plants to investigate how rootstock cotyledon affects healing.The complete removal of rootstock cotyledons significantly hindered scion growth,as evidenced by reductions in scion fresh weight and the area of true leaves.Physiological assessments revealed reduced callus formation,weaker adhesion forces,a more pronounced necrotic layer,and decreased rates of xylem and phloem reconnection at the graft junction when rootstock cotyledons were completely removed.Additionally,auxin levels at the rootstock graft junction notably decreased following cotyledon removal.In contrast,the exogenous application of indole-3-acetic acid(IAA)notably enhanced graft healing.Moreover,gene expression analysis of the PIN auxin efflux carriers in the rootstock cotyledons indicated significant activation of ClPIN1a postgrafting.Furthermore,we developed an improved Virus-Induced Gene Silencing(VIGS)system for cucurbits using seeds soaking method.This method achieved an infection success rate of 83%with 60%-75%gene silencing efficiency,compared to the 37%success rate with 40%-60%efficiency seen with traditional cotyledon infection.Combining our novel VIGS approach with cotyledon grafting techniques,we demonstrated that rootstock cotyledons regulate callus formation through ClPIN1a-mediated endogenous auxin release,thus facilitating graft union development.These findings suggest potential strategies for enhancing watermelon graft healing by manipulating rootstock cotyledons.展开更多
The experiment evaluated the impact of several commercial rootstocks on the growth and yield of a selected tomato cultivar under restricted volume conditions.The experiment included six different rootstocks and a sing...The experiment evaluated the impact of several commercial rootstocks on the growth and yield of a selected tomato cultivar under restricted volume conditions.The experiment included six different rootstocks and a single tomato cultivar(Solanum lycopersicum cv.Izmir).The respective rootstock-scion combination and self-grafted tomato plants were tested in a randomized complete block design.The plants,transplanted into 5 L plastic pots,were irrigated daily with equal amounts of a nutrient solution and were subject to two different levels of salinity,0-or 50-mM NaCl solution,respectively.The relative stem elongation rate and new leaf emergence rate were calculated from day 1 to day 42 after transplanting,and nutrient concentrations of mature leaves were analyzed by the end of this period.Fruits were harvested at full maturity,and Brix(°Bx)and fruit acidity were measured using a digital refractometer.While the Na concentration of mature leaves significantly increased,the rise of salinity in the nutrient solution significantly reduced the plants'relative growth rate,K and P concentration of mature leaves,and the yield of tomato plants.Although the grafted plants demonstrated improved shoot growth and increased new leaf emergence rate,indicating enhanced capabilities to alleviate salinity stress,most of the rootstocks did not contribute to any increase in tomato yield.Since only carefully selected rootstock-scion combinations might contribute to higher yields and better quality,the introduction of new rootstocks should be followed by continuous testing of their compatibility with specific,well-recognized tomato cultivars under diverse growing conditions.展开更多
[Objectives]To select the virus-free Citrus reticulata Hongmeiren as test material to select rootstocks suitable for local cultivation,and to carry out demonstration and popularization of suitable rootstocks for plant...[Objectives]To select the virus-free Citrus reticulata Hongmeiren as test material to select rootstocks suitable for local cultivation,and to carry out demonstration and popularization of suitable rootstocks for planting C.reticulata Hongmeiren in the south bank of Hangzhou Bay.[Methods]The effects of different rootstocks on the survival rate,biological characteristics and fruit quality were analyzed by grafting onto three kinds of rootstocks,namely,C.unshiu Marc.cv.Owari,S.mandarin cv.Miyagawa wase and C.trifoliata L.[Results]The grafting survival rate for C.trifoliata L.was the highest;from the index of scion growth,the scion diameter,new shoot length,new shoot thickness and leaf area of C.unshiu Marc.cv.Owari were the largest,and the tree height of S.mandarin cv.Miyagawa wase was the highest;from the fruit quality index,the fruit quality of C.unshiu Marc.cv.Owari was the best.[Conclusions]C.unshiu Marc.cv.Owari is suitable as the preferred rootstock for virus-free C.reticulata Hongmeiren on the south bank of Hangzhou Bay,and can be demonstrated and promoted.展开更多
The aim of this study was to investigate the impact of heat stress on physiological features, together with endogenous hormones and the transcription level of related genes, to estimate the heat resistance ability and...The aim of this study was to investigate the impact of heat stress on physiological features, together with endogenous hormones and the transcription level of related genes, to estimate the heat resistance ability and stress injury mechanism of different dwarfing apple rootstocks. Among the six rootstocks, the rootstocks of native Shao series(SH series) showed better heat stress resistance than those of Budagovski 9(B9), Cornell-Geneva 24(CG24), and Malling 26(M26) from abroad. Among SH series rootstocks, SH1 and SH6 showed higher heat stress resistance than SH40. M26 demonstrated the lowest adaption ability to heat stress, showing higher leaf conductivity and lower liquid water content(LWC) with the increase in temperature. Heat stress also resulted in the suppression of photosynthesis, which showed no significant restoration after 7-day recovery. It should be noted that although a higher temperature led to a lower LWC and photosynthetic efficiency(P_n) of CG24, there was no significant increase in leaf conductivity, and 7 days after the treatment, the P_n of CG24 recovered. The extremely high temperature tolerance of SH series rootstocks could be related to the greater osmotic adjustment(OA), which was reflected by smaller reductions in leaf relative water content(RWC) and higher turgor potentials and leaf gas exchange compared with the other rootstocks. Determination of hormones indicated multivariate regulation, and it is presumed that a relatively stable expression levels of functional genes under high-temperature stress is necessary for heat stress resistance of rootstocks.展开更多
US pear production is constrained by the lack of dwarfing and precocious rootstocks that revolutionized other fruit crops,such as apple.While quince is used as a rootstock in global pear production,concerns about pote...US pear production is constrained by the lack of dwarfing and precocious rootstocks that revolutionized other fruit crops,such as apple.While quince is used as a rootstock in global pear production,concerns about potential graft incompatibility and lack of cold hardiness limit its adoption in the US.This work was aimed at identifying genetic determinants of dwarfing in Pyrus backgrounds to inform future breeding for dwarfing Pyrus rootstocks.In 2018,145 rootstock seedlings of two reciprocal crosses were budded with a standard scion variety.Rootstock seedlings were also genotyped with the 70 K Pyrus SNP array.Based on two-year orchard architectural phenotypes,quantitative trait loci(QTLs)were consistently mapped on both chromosomes 5 and 15 for dwarfing traits,namely scion trunk cross-sectional area(TCA),total scion annual growth,and central leader annual growth.QTLs for rootstock TCA were also detected on both chromosomes 5 and 15;however,the chromosome 15 QTL did not co-localize with the scion trait locus.Each dwarfing haplotype accounted for 30% to 50% reduction in vigor(p<0.05).Combined haplotype analysis showed that one dwarfing locus was sufficient to significantly reduce vigor.Presence of two dwarfing haplotypes further reduced vigor by a total of 50% to 70%(p<0.05),but their combinatory effects were not purely additive due to epistasis.Discovery of these novel dwarfing loci(named P×Dwg1 and P×Dwg2)in Pyrus facilitates future DNA test development to enable informed parental and seedling selection for dwarfing potential.展开更多
A study was conducted in attempting to identify the cold-resistant apple rootstocks and to establish a comprehensive evaluation system. In this study, 10 elite apple dwarfing rootstocks(GM256, JM7, M26, M7, SC1, SH1, ...A study was conducted in attempting to identify the cold-resistant apple rootstocks and to establish a comprehensive evaluation system. In this study, 10 elite apple dwarfing rootstocks(GM256, JM7, M26, M7, SC1, SH1, SH38, SH6, M9, and T337) were employed for the experiment and the following parameters were investigated under different low temperature stress conditions(0, –15, –20, –25, –30, and –35°C): the changes of the relative electrical conductivity(REC), anthocyanin content, protein content, soluble sugar content, soluble starch content, proline content, malondialdehyde(MDA) content, superoxide dismutase(SOD) activity, and peroxidase(POD) activity of the dormant branches. The inflection temperature that could represent the plant tissue semi-lethal temperature(LT) was obtained by the measurements of REC. The LTwas used to evaluate eight other indices. The results showed that there was no significant correlation between LTand POD activity as well as between the soluble sugar, protein and proline contents at 0 and –15°C. Soluble starch content at 0 and –15°C and anthocyanin content at –15–(–30)°C were significantly but negatively correlated to the LT50 and the MDA content at 0–(–20)°C was significantly positively correlated to the LT. Statistical analysis based on principal component analysis and LT50 showed that cold resistant apple rootstocks in the decreasing order from high to low as GM256, SH6, SH38, SH1, SC1, M26, M7, JM7, T337, and M9.展开更多
Alkaline soils have a great inlfuence on apple production in Northern China. Therefore, comprehensive evaluations of toler-ance to such stress are important when selecting the most suitable apple rootstocks. We used h...Alkaline soils have a great inlfuence on apple production in Northern China. Therefore, comprehensive evaluations of toler-ance to such stress are important when selecting the most suitable apple rootstocks. We used hydroponics culturing to test 17 genotypes of apple rootstocks after treatment with 1:1Na2CO3and NaHCO3. When compared with the normaly grown controls, stressed plants produced fewer new leaves, and had shorter roots and shoots and lower fresh and dry weights after 15 d of exposure to alkaline conditions. Their root/shoot ratios were also reduced, indicating that the roots had been severely damaged. For al stressed rootstocks, electrolyte leakage (EL) and the concentration of malondialdehyde (MDA) increased while levels of chlorophyl decreased. Changes in root activity (up or down), as wel as the activities of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were rootstock-dependent, possibly relfecting their differences in alkali tolerance. Using alkali injury index (AI), adversity resistance coefifcients (ARC),cluster analysis, and evaluation of their physiological responses, we classiifed these 17 genotypes into three groups: (1) high tolerance: Hubeihaitang, Wushanbianyehaitang, Laoshanhaitang Ls2, Xiaojinbianyehaitang, and Fupingqiuzi; (2) moderate tolerance: Pingyitiancha, Laoshanhaitang Ls3, Hubeihaitang A1, Deqinhaitang, Balenghaitang, Maoshandingzi, Shandingzi, and Xinjiangyepingguo; or (3) low tolerance: Pingdinghaitang, Hongsanyehaitang, Xiaojinhaitang, and Sanyehaitang. These results wil signiifcantly contribute to the selection of the most suitable materials for rootstocks with desired levels of tolerance to alkali stress.展开更多
Vegetable grafting has become an important method for developing resistance to biotic and abiotic stresses and increasing potential yield in agronomic practice.To determine the effects of grafting on tomato taste,we e...Vegetable grafting has become an important method for developing resistance to biotic and abiotic stresses and increasing potential yield in agronomic practice.To determine the effects of grafting on tomato taste,we examined the cultivated tomato‘Moneymaker’and the wild tomato Solanum pimpinellifolium,which have different fruit weights and qualities,to investigate the effect of grafting on sugars,acids,and volatiles in single-head and double-head grafted plants using GC-MS and LC-MS.We observed that the contents of sugars,acids and volatiles in heterografted tomato pericarps are influenced by rootstocks.Different metabolites exhibit distinct responses to the rootstock and might be associated with rootstock-scion interactions.Comparison of the metabolites in the heterogeneous fruit of the single-and double-head grafted plants indicates that the grafting method also influences the metabolic changes in grafted plants.Moreover,we also identified numerous mobile transcripts and determined that the mobile mRNAs are associated with metabolic processes in tomato leaves.Our work helps to elucidate the effect of genotype and graft method on fruit quality and may provide a basis for future research on rootstock breeding and selection in plants.展开更多
Adventitious root formation is a bottleneck during vegetative proliferation.Potassium(K+)is an essential macronutrient for plants.K+accumulation from the soil and its distribution to the different plant organs i...Adventitious root formation is a bottleneck during vegetative proliferation.Potassium(K+)is an essential macronutrient for plants.K+accumulation from the soil and its distribution to the different plant organs is mediated by K+transporters named K+transporter(KT),K+uptake(KUP),or high-affinity K+(HAK).This study aimed to identify members of the HAK gene family in apples and to characterize the effects of K+supply on adventitious root formation and on the expression of HAK genes and the genes that putatively control auxin transport,signaling,and cell fate during adventitious root formation.In this study,34 HAK genes(MdHAKs)were identified in the apple(Malus×domestica‘Golden Delicious’)genome.A phylogenetic analysis divided MdHAKs into four clusters(Ⅰ,Ⅱ,Ⅲ,andⅣ),comprising 16,1,4,and 13 genes,respectively.The syntenic relationships revealed that 62.5%of the total MdHAK genes arise from genomic duplication events.Chromosome location,domain structure,motif analysis,and physico-chemical characteristics were subsequently investigated.Furthermore,the application of K+indicated the emergence of adventitious roots at 8 d and produced more adventitious roots at 16 d than the K+-free control(CK)treatment.In addition,various MdHAKs showed root-specific expression in B9 apple rootstock stem cuttings and enhanced expression during the initiation and emergence stages of adventitious root formation in response to K+treatment.Additionally,K+treatment enhanced the expression levels of MdPIN1,MdPIN2,and MdAUX1.Further data indicated that a higher expression of MdWOX11,MdLBD16,and MdLBD29 and of cell cycle-related genes contributed to the auxin-stimulated adventitious root formation in response to K+.展开更多
Dear Editor,Cabernet Sauvignon is one of the most economically valuable and widely planted wine grape varieties.It is widely planted in Xinjiang,an important grapeproducing area in China.Grafting,an ancient agricultur...Dear Editor,Cabernet Sauvignon is one of the most economically valuable and widely planted wine grape varieties.It is widely planted in Xinjiang,an important grapeproducing area in China.Grafting,an ancient agricultural technique,is widely used to improve the biotic and abiotic resistance of grapes.展开更多
The endophytic microbiome of plants is believed to have a significant impact on its physiology and disease resistance,however,the role of host genotype in determining the composition of the endophytic microbiome of ap...The endophytic microbiome of plants is believed to have a significant impact on its physiology and disease resistance,however,the role of host genotype in determining the composition of the endophytic microbiome of apple root systems remains an open question that has important implications for defining breeding objectives.In the current study,the bacterial and fungal microbiota associated with four different apple rootstocks planted in April,2018 in the same soil environment and harvested in May,2019 were evaluated to determine the role of genotype on the composition of both the bacterial and fungal communities.Results demonstrated a clear impact of genotype and root size on microbial composition and diversity.The fungalcommunitywasmore affected by plant genotype whereas the bacterial community was shaped by root size.Fungal and bacterial abundance was equal between different-sized roots however,significantly higher microbial counts were detected in rhizosphere samples compared to root endosphere samples.This study provides information that can be used to develop a comprehensive and readily applicable understanding of the impact of genotype and environmental factors on the establishment of plant microbiome,as well as its potential function and impact on host physiology.展开更多
[Objective] The paper aims to study the effects of drought stress on antioxidation system of different Citrus rootstock leaves. [Method] Potted plant experiments were carried out to control the water content of soil i...[Objective] The paper aims to study the effects of drought stress on antioxidation system of different Citrus rootstock leaves. [Method] Potted plant experiments were carried out to control the water content of soil in pots,in order to investigate the effects of drought stress on antioxidation system activity (SOD,POD and CAT) and the content of protein,GSH,MDA and O-·2 from the leaves of Shandong Zhike,Ningminju,Yangshuojinbaosuanju,Huapijinju and Guizhi No.1. [Result] The content of chlorophyll a,chlorophyll b and chlorophyll (a+b) from the test 5 breeds decreased with the enhancement of drought stress,and there were significant differences between them and the control under the severe stress; the activities of SOD,POD and CAT from the 5 breeds increased as the drought stress strengthened; the content of soluble protein declined while that of GSH,MDA and O-·2 rose because of the drought stress. [Conclusion] This research provided a good reference to choose the breeds of citrus rootstocks in arid and semi-arid areas.展开更多
The drought resistance of eight commonly used apple rootstocks under natural drought conditions was examined to provide clues for the selection,promotion,and utilization of drought-resistant apple rootstocks.The ultra...The drought resistance of eight commonly used apple rootstocks under natural drought conditions was examined to provide clues for the selection,promotion,and utilization of drought-resistant apple rootstocks.The ultrastructural differences and physiological and biochemical characteristics of the leaves of eight apple rootstocks under drought stress were observed.The index changes were used to rank drought resistance by the membership function method comprehensively.The results showed that the leaf thickness,palisade tissue thickness,sponge tissue thickness,net photosynthetic rate,and chlorophyll content were significantly higher in‘Fupingqiuzi'than those of other rootstocks at various stress conditions.The leaf water content and water use efficiency of'Qingzhen 1'were significantly higher than those of other rootstocks under different stress conditions.The root vigor of‘B.9'was significantly higher than that of other rootstocks.The results of membership function analysis showed that the drought resistance of different rootstocks was in the order:‘Fupingqiuzi'>‘Qingzhen 1'>‘B.9'>‘MM.111'>‘GM256'>‘MM.106'>‘M.26'>‘M.9-Nic29'.‘Fupingqiuzi'had the strongest drought resistance,and‘Qingzhen 1'and‘B.9'were also relatively droughtresistant.These rootstocks can be used as raw materials for drought-resistant apple rootstock breeding and are propagated and utilized in arid areas.展开更多
[Objective] Iron deficiency is one of the most important crop element deficiencies in the Loess Plateau of northwestern China. The selection for crop cultivars that are tolerant to low iron levels could be one of the ...[Objective] Iron deficiency is one of the most important crop element deficiencies in the Loess Plateau of northwestern China. The selection for crop cultivars that are tolerant to low iron levels could be one of the approaches to solving the problem and improving crop production. [Method] Three major apple root stock species (Malus prunifolia, Malus sieversii and Malus baccata) were selected to evaluate their tolerance to iron defciency in hydroponic system. A 3×2 factorial pot experiment was conducted with three replicates in a greenhouse at Gansu Agricultural University, Lanzhou, China. [Result] The SOD, POD and CAT activities in roots and stems of the 3 root stock species in Fe-defcient Hoagland solution decreased, however Malus sieversii got the less reduction and had better root architecture and growth than the other species. The aboveground biomass, plant height, chlorophyll content, total root length and lateral root number were correlated positively with iron-defciency stress tolerance. The species’ tolerance to iron-defciency from high to low was M. sieversii’s〉M. baccata’s〉M. prunifolia’s. Moreover, the improvement of some morphological features such as root length, above-ground biomass, plant height and lateral root number in apple could be conducive to breeding cultivars with tolerance to iron-defciency stress. [Conclusion] Malus sieversii had better tolerance to iron-defciency stress than the others in this study.展开更多
[Ohjective] The aim of the study is to investigate the productivity and light quality in two high density M26 dwarf rootstock apple orchards in central China so as to provide some management guidance for close plantin...[Ohjective] The aim of the study is to investigate the productivity and light quality in two high density M26 dwarf rootstock apple orchards in central China so as to provide some management guidance for close planting of dwarf rootstock apples.[Method] The technical parameters of individual trees and group parameters as shoot number and composition and canopy coverage were determined, and the light quality in the canopy, fruit production and quality were investigated. [Result] Slender spindle (SS) orchard has 54 thousands shoots per 667 m^2. Coverage rate is 76%. Leaf area index is 1.9. The ratio of long, medium and spur shoots is 1:1:8. Fruit yield is 3 263 kg/667 m^2 with 85% first grade fruit. Light interception in the canopy is 58% while the ratio of canopy with good light is 65%. Modified slender spindle (MSS) orchard has 93 thousands shoots per 667 m^2 and the coverage is 77%. Leaf area index is 3.3. The ratio cf long, medium and spur shoots is 1:2:7. Fruit yield is 3 931 kg/667 m^2 with 85% first grade fruit. The light interception in the canopy is 73% while the ratio of canopy with good light is 35%. [Conclusion] Apple orchard with M26 dwarf rootstock trained as SS and MSS tree form in medium planting density may be useful to the management of the similar orchards in Central China.展开更多
Grafting is a common technique used to enhance watermelon yield under biotic and abiotic stress conditions;however,it may influence fruit development and quality.This study evaluated the impact of grafting on the frui...Grafting is a common technique used to enhance watermelon yield under biotic and abiotic stress conditions;however,it may influence fruit development and quality.This study evaluated the impact of grafting on the fruit quality attributes and volatile profiles of'Ningnongke huadai'watermelon using 11 commercial rootstocks,encompassing wild watermelons,bottle gourds,and pumpkins.Significant variations were observed in fruit weight,length,rind thickness,firmness of rind and flesh,and central soluble solids content.Notably,wild watermelon grafting increased central soluble solids,while pumpkin-grafted fruits exhibited higher fruit weight but lower central soluble solids.The analysis identified 122 volatile compounds in watermelon samples,primarily ketones,aldehydes,and alcohols.Regarding volatile composition,bottle gourd and wild watermelon grafts did not significantly differ from non-grafted watermelons,whereas pumpkin-grafted fruits showed distinctive volatile profiles characterized by higher aldehyde content and lower levels of alcohols and aromatic hydrocarbons.In conclusion,wild watermelon rootstocks increased soluble solids and had minimal impact on the volatile profiles of grafted fruits,making them potentially suitable for commercial production of'Ningnongke huadai'watermelon.The evident variability in volatile profiles among grafting combinations underscores the need for watermelon rootstock breeding programs to account for the influence of rootstocks on fruit aroma.展开更多
Soil alkalinity is a major factor that restricts the growth of apple roots.To analyze the response of apple roots to alkali stress, the root structure and endogenous hormones of two apple rootstocks, Malus prunifolia ...Soil alkalinity is a major factor that restricts the growth of apple roots.To analyze the response of apple roots to alkali stress, the root structure and endogenous hormones of two apple rootstocks, Malus prunifolia (alkali-tolerant) and Malus hupehensis (alkali-sensitive), were compared. To understand alkali tolerance of M. prunifolia at the molecular level, transcriptome analysis was performed. When plants were cultured in alkaline conditions for 15 d, the root growth of M. hupehensis with weak alkali tolerance decreased significantly. Analysis of endogenous hormone levels showed that the concentrations of indole-3-acetic acid (IAA) and zeatin riboside (ZR) in M. hupehensis under alkali stress were lower than those in the control. However, the trend for IAA and ZR in M. prunifolia was the opposite. The concentration of abscisic acid (ABA) in the roots of the two apple rootstocks under alkali stress increased, but the concentration of ABA in the roots of M. prunifolia was higher than that in M. hupehensis. The expression of IAA-related genes ARF5, GH3.6, SAUR36, and SAUR32 and the Cytokinin (CTK)-related gene IPT5 in M. prunifolia was higher than those in the control, but the expression of these genes in M. hupehensis was lower than those in the control. The expression of ABA-related genes CIPK1 and AHK1 increased in the two apple rootstocks under alkali stress, but the expression of CIPK1 and AHK1 in M. prunifolia was higher than in M. hupehensis. These results demonstrated that under alkali stress, the increase of IAA, ZR, and ABA in roots and the increase of the expression of related genes promoted the growth of roots and improved the alkali tolerance of apple rootstocks.展开更多
Selection of rootstock is very important for citrus production. Besides its major role on resistance, the rootstock also can affect fruit production and quality. Currently, the main concerns on selection of rootstock ...Selection of rootstock is very important for citrus production. Besides its major role on resistance, the rootstock also can affect fruit production and quality. Currently, the main concerns on selection of rootstock for citrus production are compatibility and resistance, due to less information on the impacts of rootstock to the performance of scion varieties. This study aims to provide information on performances of navel orange varieties on different rootstocks. Three late-ripening navel orange varieties(Citrus sinensis var. Powell, Chislett and Banfield) grafted on seven rootstocks(Swingle citrumelo(C. paradisi× P. trifoliata), Carrizo citrange(C. sinensis×P. trifoliata), X639(C. reticulata×P. trifoliata), MXT(C. sinensis×P. trifoliata), Hongju(C. reticulata), Ziyang Xiangcheng(C. junos) and trifoliate orange(P. trifoliata)) were used as plant materials for comprehensive comparison of the performances on tree growth, fruit yield and quality in 21 scion–stock combinations. Investigation was carried out in these combinations in field nine years after planting. Vigorous growth of all the three late-ripening navel orange varieties was observed on Carrizo citrange with the largest canopy volume at 33.34 m3 and the highest yield at 29.43 kg per tree, but a low yield efficiency at 2.87 kg m–3. On the contrary, those on trifoliate orange had the smallest canopy volume at 10.79 m3 and the lowest fruit yield at 12.51 kg per tree, but the highest yield efficiency at 3.95 kg m–3. Rootstocks did not show significant effects on fruit size, fruit shape index, peel thickness and the edible rate of the fruits, but fruit quality was significantly affected by the rootstocks. Fruits from the trees grafted on trifoliate orange presented the best quality with significantly higher total soluble solids(TSS) content than those on Ziyang Xiangcheng and Hongju, and also the highest ratio of TSSitratable acidity(TA). The TA content was observed from the fruits on X639 at 0.59 g 100 mL–1. Vitamin C(Vc) content of fruits on Hongju was the highest at 49.25 mg 100 mL–1. Growth vigor of the trees was positively correlated with fruit yield at an extremely significant level. The canopy volume was negatively correlated with yield efficiency, but positively correlated with compatibility index. Results of this study indicated that the rootstock has great impacts on the growth vigor of the tree, yield efficiency and quality of the fruit. In order to achieve good quality and yield efficiency for navel orange production, less growth vigor rootstock such as trifoliate orange is highly recommended.展开更多
基金supported by the“Guizhou Provincial Major Scientifc and Technological Program(Qiankehe[2024]026-2-2)”“Guizhou Provincial Scientific and Technological Program(QKHFQ[2024]004-1)”.
摘要Rootstock plays an important role in commercial horticultural production by influencing scion growth,physiology,and fruit quality.However,information on the effects of different rootstocks on kiwifruit growth and fruit quality remains limited.In this study,Actinidia valvata was evaluated as an experimental rootstock and compared with the commonly used A.deliciosa rootstock to investigate their effects on photosynthetic characteristics,carbohydrate metabolism,and fruit quality of A.deliciosa cv.‘Guichang’.Leaf photosynthetic parameters,fruit quality attributes,carbohydrate contents,and the activities of enzymes related to sucrose and starch metabolism were investigated during fruit development and postharvest ripening.The results showed that vines grafted onto A.valvata exhibited higher photosynthetic performance at certain developmental stages.Favorable changes were also detected in several physiological indicators associated with starch and sugar accumulation.In addition,fruits from plants grafted onto A.valvata exhibited enhanced activities of key enzymes involved in sucrose and starch metabolism at specific sampling stages.However,for many measured variables and time points,no significant differences were detected between the two rootstocks.Overall,these results suggest that A.valvata has potential as an alternative rootstock for‘Guichang’kiwifruit,particularly with respect to carbohydrate metabolism,although its overall performance was broadly comparable to that of A.deliciosa.
基金supported by the National Key Research and Development Program of China(2022YFD1201700)National Natural Science Foundation of China(32172538)thw Key Research and Development Plan(Major Scientific and Technological Innovation Project)of Shandong Province(2022LZGC010).
摘要In rootstock-scion combinations,the rootstock is the key factor determining a plant's salt tolerance,primarily through its root physiology and soil interaction capabilities.However,the interaction between rootstock and scion in influencing salt tolerance in apple trees remains underexplored.In this study,three one-year-old dwarf rootstocks—'Qingzhen-1','M9-T337',and'(Malus hupehensis Rehd,var.Pingyiensis,PYTC)'—were selected,with'Luli'used as the scion.The salt tolerance was compared in natural soil(1‰),and saline-alkali soil from Dongying(3‰,6‰,10‰).The results showed that salt stress inhibited plant growth and damaged root structure.Among the combinations,Luli/Qingzhen-1 exhibited superior growth performance,with reduced malondialdehyde content,relative conductivity,and mortality.Additionally,in the Luli/Qingzhen-1 combination,genes associated with antioxidant enzyme activity and stress signal transduction were significantly upregulated.In conclusion,Qingzhen-1 demonstrates greater salt tolerance than M9-T337 and PYTC,making it a promising rootstock for cultivating'Luli'scions in saline-alkali soils.
基金supported by the National Natural Science Foundation of China(31972434)the China Agriculture Research System of MOF and MORA(CARS-25)+4 种基金the Hubei Provincial Key Research and Development Program(2023BBB033)the Fundamental Research Funds for the Central Universities(2662024JC004)the Young Scientist Forstering Funds for the National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops(11909920008)the Huazhong Agricultural UniversityAgricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences Cooperation Fund(SZYJY2021005)Shannan Science and Technology Plan Project(SNSBJKJJHXM2023004).
摘要Grafting is a propagation method extensively utilized in cucurbits.However,the mechanisms underlying graft healing remain poorly understood.This study employed self-grafted watermelon plants to investigate how rootstock cotyledon affects healing.The complete removal of rootstock cotyledons significantly hindered scion growth,as evidenced by reductions in scion fresh weight and the area of true leaves.Physiological assessments revealed reduced callus formation,weaker adhesion forces,a more pronounced necrotic layer,and decreased rates of xylem and phloem reconnection at the graft junction when rootstock cotyledons were completely removed.Additionally,auxin levels at the rootstock graft junction notably decreased following cotyledon removal.In contrast,the exogenous application of indole-3-acetic acid(IAA)notably enhanced graft healing.Moreover,gene expression analysis of the PIN auxin efflux carriers in the rootstock cotyledons indicated significant activation of ClPIN1a postgrafting.Furthermore,we developed an improved Virus-Induced Gene Silencing(VIGS)system for cucurbits using seeds soaking method.This method achieved an infection success rate of 83%with 60%-75%gene silencing efficiency,compared to the 37%success rate with 40%-60%efficiency seen with traditional cotyledon infection.Combining our novel VIGS approach with cotyledon grafting techniques,we demonstrated that rootstock cotyledons regulate callus formation through ClPIN1a-mediated endogenous auxin release,thus facilitating graft union development.These findings suggest potential strategies for enhancing watermelon graft healing by manipulating rootstock cotyledons.
摘要The experiment evaluated the impact of several commercial rootstocks on the growth and yield of a selected tomato cultivar under restricted volume conditions.The experiment included six different rootstocks and a single tomato cultivar(Solanum lycopersicum cv.Izmir).The respective rootstock-scion combination and self-grafted tomato plants were tested in a randomized complete block design.The plants,transplanted into 5 L plastic pots,were irrigated daily with equal amounts of a nutrient solution and were subject to two different levels of salinity,0-or 50-mM NaCl solution,respectively.The relative stem elongation rate and new leaf emergence rate were calculated from day 1 to day 42 after transplanting,and nutrient concentrations of mature leaves were analyzed by the end of this period.Fruits were harvested at full maturity,and Brix(°Bx)and fruit acidity were measured using a digital refractometer.While the Na concentration of mature leaves significantly increased,the rise of salinity in the nutrient solution significantly reduced the plants'relative growth rate,K and P concentration of mature leaves,and the yield of tomato plants.Although the grafted plants demonstrated improved shoot growth and increased new leaf emergence rate,indicating enhanced capabilities to alleviate salinity stress,most of the rootstocks did not contribute to any increase in tomato yield.Since only carefully selected rootstock-scion combinations might contribute to higher yields and better quality,the introduction of new rootstocks should be followed by continuous testing of their compatibility with specific,well-recognized tomato cultivars under diverse growing conditions.
摘要[Objectives]To select the virus-free Citrus reticulata Hongmeiren as test material to select rootstocks suitable for local cultivation,and to carry out demonstration and popularization of suitable rootstocks for planting C.reticulata Hongmeiren in the south bank of Hangzhou Bay.[Methods]The effects of different rootstocks on the survival rate,biological characteristics and fruit quality were analyzed by grafting onto three kinds of rootstocks,namely,C.unshiu Marc.cv.Owari,S.mandarin cv.Miyagawa wase and C.trifoliata L.[Results]The grafting survival rate for C.trifoliata L.was the highest;from the index of scion growth,the scion diameter,new shoot length,new shoot thickness and leaf area of C.unshiu Marc.cv.Owari were the largest,and the tree height of S.mandarin cv.Miyagawa wase was the highest;from the fruit quality index,the fruit quality of C.unshiu Marc.cv.Owari was the best.[Conclusions]C.unshiu Marc.cv.Owari is suitable as the preferred rootstock for virus-free C.reticulata Hongmeiren on the south bank of Hangzhou Bay,and can be demonstrated and promoted.
基金support of the Special Fund for the China Agriculture Research System (CARS-28)the Special Fund for the Construction of Scientific and Technological Innovation Capability, China (KJXC20140406)
摘要The aim of this study was to investigate the impact of heat stress on physiological features, together with endogenous hormones and the transcription level of related genes, to estimate the heat resistance ability and stress injury mechanism of different dwarfing apple rootstocks. Among the six rootstocks, the rootstocks of native Shao series(SH series) showed better heat stress resistance than those of Budagovski 9(B9), Cornell-Geneva 24(CG24), and Malling 26(M26) from abroad. Among SH series rootstocks, SH1 and SH6 showed higher heat stress resistance than SH40. M26 demonstrated the lowest adaption ability to heat stress, showing higher leaf conductivity and lower liquid water content(LWC) with the increase in temperature. Heat stress also resulted in the suppression of photosynthesis, which showed no significant restoration after 7-day recovery. It should be noted that although a higher temperature led to a lower LWC and photosynthetic efficiency(P_n) of CG24, there was no significant increase in leaf conductivity, and 7 days after the treatment, the P_n of CG24 recovered. The extremely high temperature tolerance of SH series rootstocks could be related to the greater osmotic adjustment(OA), which was reflected by smaller reductions in leaf relative water content(RWC) and higher turgor potentials and leaf gas exchange compared with the other rootstocks. Determination of hormones indicated multivariate regulation, and it is presumed that a relatively stable expression levels of functional genes under high-temperature stress is necessary for heat stress resistance of rootstocks.
基金funded by Fresh and Processed Pear Committee Research(PR-09-905,PR-15-105,PR-19-108,and PR-22-102)USDA National Institute of Food and Agriculture Hatch project 1014919.
摘要US pear production is constrained by the lack of dwarfing and precocious rootstocks that revolutionized other fruit crops,such as apple.While quince is used as a rootstock in global pear production,concerns about potential graft incompatibility and lack of cold hardiness limit its adoption in the US.This work was aimed at identifying genetic determinants of dwarfing in Pyrus backgrounds to inform future breeding for dwarfing Pyrus rootstocks.In 2018,145 rootstock seedlings of two reciprocal crosses were budded with a standard scion variety.Rootstock seedlings were also genotyped with the 70 K Pyrus SNP array.Based on two-year orchard architectural phenotypes,quantitative trait loci(QTLs)were consistently mapped on both chromosomes 5 and 15 for dwarfing traits,namely scion trunk cross-sectional area(TCA),total scion annual growth,and central leader annual growth.QTLs for rootstock TCA were also detected on both chromosomes 5 and 15;however,the chromosome 15 QTL did not co-localize with the scion trait locus.Each dwarfing haplotype accounted for 30% to 50% reduction in vigor(p<0.05).Combined haplotype analysis showed that one dwarfing locus was sufficient to significantly reduce vigor.Presence of two dwarfing haplotypes further reduced vigor by a total of 50% to 70%(p<0.05),but their combinatory effects were not purely additive due to epistasis.Discovery of these novel dwarfing loci(named P×Dwg1 and P×Dwg2)in Pyrus facilitates future DNA test development to enable informed parental and seedling selection for dwarfing potential.
基金supported by the Fundamental Research Funds for the Universities of Gansu Province,China(035-041051)the Natural Science Foundation of Gansu Province,China(145RJZA167)
摘要A study was conducted in attempting to identify the cold-resistant apple rootstocks and to establish a comprehensive evaluation system. In this study, 10 elite apple dwarfing rootstocks(GM256, JM7, M26, M7, SC1, SH1, SH38, SH6, M9, and T337) were employed for the experiment and the following parameters were investigated under different low temperature stress conditions(0, –15, –20, –25, –30, and –35°C): the changes of the relative electrical conductivity(REC), anthocyanin content, protein content, soluble sugar content, soluble starch content, proline content, malondialdehyde(MDA) content, superoxide dismutase(SOD) activity, and peroxidase(POD) activity of the dormant branches. The inflection temperature that could represent the plant tissue semi-lethal temperature(LT) was obtained by the measurements of REC. The LTwas used to evaluate eight other indices. The results showed that there was no significant correlation between LTand POD activity as well as between the soluble sugar, protein and proline contents at 0 and –15°C. Soluble starch content at 0 and –15°C and anthocyanin content at –15–(–30)°C were significantly but negatively correlated to the LT50 and the MDA content at 0–(–20)°C was significantly positively correlated to the LT. Statistical analysis based on principal component analysis and LT50 showed that cold resistant apple rootstocks in the decreasing order from high to low as GM256, SH6, SH38, SH1, SC1, M26, M7, JM7, T337, and M9.
基金supported by the National Natural Science Foundation of China (31401830)the Chinese Universities Scientific Fund (QN2011006)the Talent Special Fund of Northwest A&F University, China (Z111020904)
摘要Alkaline soils have a great inlfuence on apple production in Northern China. Therefore, comprehensive evaluations of toler-ance to such stress are important when selecting the most suitable apple rootstocks. We used hydroponics culturing to test 17 genotypes of apple rootstocks after treatment with 1:1Na2CO3and NaHCO3. When compared with the normaly grown controls, stressed plants produced fewer new leaves, and had shorter roots and shoots and lower fresh and dry weights after 15 d of exposure to alkaline conditions. Their root/shoot ratios were also reduced, indicating that the roots had been severely damaged. For al stressed rootstocks, electrolyte leakage (EL) and the concentration of malondialdehyde (MDA) increased while levels of chlorophyl decreased. Changes in root activity (up or down), as wel as the activities of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were rootstock-dependent, possibly relfecting their differences in alkali tolerance. Using alkali injury index (AI), adversity resistance coefifcients (ARC),cluster analysis, and evaluation of their physiological responses, we classiifed these 17 genotypes into three groups: (1) high tolerance: Hubeihaitang, Wushanbianyehaitang, Laoshanhaitang Ls2, Xiaojinbianyehaitang, and Fupingqiuzi; (2) moderate tolerance: Pingyitiancha, Laoshanhaitang Ls3, Hubeihaitang A1, Deqinhaitang, Balenghaitang, Maoshandingzi, Shandingzi, and Xinjiangyepingguo; or (3) low tolerance: Pingdinghaitang, Hongsanyehaitang, Xiaojinhaitang, and Sanyehaitang. These results wil signiifcantly contribute to the selection of the most suitable materials for rootstocks with desired levels of tolerance to alkali stress.
基金supported by the National Key Research and Development Program of China (Grant No. 2018YFD1000801)the Central Public-interest Scientific Institution Basal Research Fund (Grant No. Y2020PT29)the Science Technology Innovation Program of the Chinese Academy of Agricultural Sciences
摘要Vegetable grafting has become an important method for developing resistance to biotic and abiotic stresses and increasing potential yield in agronomic practice.To determine the effects of grafting on tomato taste,we examined the cultivated tomato‘Moneymaker’and the wild tomato Solanum pimpinellifolium,which have different fruit weights and qualities,to investigate the effect of grafting on sugars,acids,and volatiles in single-head and double-head grafted plants using GC-MS and LC-MS.We observed that the contents of sugars,acids and volatiles in heterografted tomato pericarps are influenced by rootstocks.Different metabolites exhibit distinct responses to the rootstock and might be associated with rootstock-scion interactions.Comparison of the metabolites in the heterogeneous fruit of the single-and double-head grafted plants indicates that the grafting method also influences the metabolic changes in grafted plants.Moreover,we also identified numerous mobile transcripts and determined that the mobile mRNAs are associated with metabolic processes in tomato leaves.Our work helps to elucidate the effect of genotype and graft method on fruit quality and may provide a basis for future research on rootstock breeding and selection in plants.
基金financially supported by the National Key Research and Development Program of China(Grant No.2018YFD1000101,2019YFD1000803)Shaanxi Apple Industry Science and Technology Project(Grant No.2020zdzx03-01-04)+1 种基金Tang Scholar by Cyrus Tang Foundation(Grant No.C200022002)The China Apple Research System(Grant No.CARS-27).
摘要Adventitious root formation is a bottleneck during vegetative proliferation.Potassium(K+)is an essential macronutrient for plants.K+accumulation from the soil and its distribution to the different plant organs is mediated by K+transporters named K+transporter(KT),K+uptake(KUP),or high-affinity K+(HAK).This study aimed to identify members of the HAK gene family in apples and to characterize the effects of K+supply on adventitious root formation and on the expression of HAK genes and the genes that putatively control auxin transport,signaling,and cell fate during adventitious root formation.In this study,34 HAK genes(MdHAKs)were identified in the apple(Malus×domestica‘Golden Delicious’)genome.A phylogenetic analysis divided MdHAKs into four clusters(Ⅰ,Ⅱ,Ⅲ,andⅣ),comprising 16,1,4,and 13 genes,respectively.The syntenic relationships revealed that 62.5%of the total MdHAK genes arise from genomic duplication events.Chromosome location,domain structure,motif analysis,and physico-chemical characteristics were subsequently investigated.Furthermore,the application of K+indicated the emergence of adventitious roots at 8 d and produced more adventitious roots at 16 d than the K+-free control(CK)treatment.In addition,various MdHAKs showed root-specific expression in B9 apple rootstock stem cuttings and enhanced expression during the initiation and emergence stages of adventitious root formation in response to K+treatment.Additionally,K+treatment enhanced the expression levels of MdPIN1,MdPIN2,and MdAUX1.Further data indicated that a higher expression of MdWOX11,MdLBD16,and MdLBD29 and of cell cycle-related genes contributed to the auxin-stimulated adventitious root formation in response to K+.
基金supported by the National Natural Science Foundation of China(31560542,32060647,32060648).
摘要Dear Editor,Cabernet Sauvignon is one of the most economically valuable and widely planted wine grape varieties.It is widely planted in Xinjiang,an important grapeproducing area in China.Grafting,an ancient agricultural technique,is widely used to improve the biotic and abiotic resistance of grapes.
基金funded equally by the U.S.-Israel Binational Agricultural Research and Development Fund(BARD)IS-5040-17 awarded to[S.D.and M.W.]the National Natural Science Foundation of China(31870675)the European Union’s Horizon2020 under“Nurturing excellence by means of cross-border and cross-sector mobility”program for MSCA-IF-2018-Individual Fellowships,grant agreement 844114[A.A.].
摘要The endophytic microbiome of plants is believed to have a significant impact on its physiology and disease resistance,however,the role of host genotype in determining the composition of the endophytic microbiome of apple root systems remains an open question that has important implications for defining breeding objectives.In the current study,the bacterial and fungal microbiota associated with four different apple rootstocks planted in April,2018 in the same soil environment and harvested in May,2019 were evaluated to determine the role of genotype on the composition of both the bacterial and fungal communities.Results demonstrated a clear impact of genotype and root size on microbial composition and diversity.The fungalcommunitywasmore affected by plant genotype whereas the bacterial community was shaped by root size.Fungal and bacterial abundance was equal between different-sized roots however,significantly higher microbial counts were detected in rhizosphere samples compared to root endosphere samples.This study provides information that can be used to develop a comprehensive and readily applicable understanding of the impact of genotype and environmental factors on the establishment of plant microbiome,as well as its potential function and impact on host physiology.
基金Supported by Natural Science Foundation in Guangxi Province(0728040)~~
摘要[Objective] The paper aims to study the effects of drought stress on antioxidation system of different Citrus rootstock leaves. [Method] Potted plant experiments were carried out to control the water content of soil in pots,in order to investigate the effects of drought stress on antioxidation system activity (SOD,POD and CAT) and the content of protein,GSH,MDA and O-·2 from the leaves of Shandong Zhike,Ningminju,Yangshuojinbaosuanju,Huapijinju and Guizhi No.1. [Result] The content of chlorophyll a,chlorophyll b and chlorophyll (a+b) from the test 5 breeds decreased with the enhancement of drought stress,and there were significant differences between them and the control under the severe stress; the activities of SOD,POD and CAT from the 5 breeds increased as the drought stress strengthened; the content of soluble protein declined while that of GSH,MDA and O-·2 rose because of the drought stress. [Conclusion] This research provided a good reference to choose the breeds of citrus rootstocks in arid and semi-arid areas.
基金supported by Shaanxi Apple Industry Science and Technology Project,Grant/Award Number:2020zdzx03-0-04Science and Technology Activity Fund for Returnees Studying Abroad of Shaanxi Province,Grant/Award Number:2020-07Cyrus Tang Foundation and the earmarked fund CARS,Grant/Award Number:CARS-27.
摘要The drought resistance of eight commonly used apple rootstocks under natural drought conditions was examined to provide clues for the selection,promotion,and utilization of drought-resistant apple rootstocks.The ultrastructural differences and physiological and biochemical characteristics of the leaves of eight apple rootstocks under drought stress were observed.The index changes were used to rank drought resistance by the membership function method comprehensively.The results showed that the leaf thickness,palisade tissue thickness,sponge tissue thickness,net photosynthetic rate,and chlorophyll content were significantly higher in‘Fupingqiuzi'than those of other rootstocks at various stress conditions.The leaf water content and water use efficiency of'Qingzhen 1'were significantly higher than those of other rootstocks under different stress conditions.The root vigor of‘B.9'was significantly higher than that of other rootstocks.The results of membership function analysis showed that the drought resistance of different rootstocks was in the order:‘Fupingqiuzi'>‘Qingzhen 1'>‘B.9'>‘MM.111'>‘GM256'>‘MM.106'>‘M.26'>‘M.9-Nic29'.‘Fupingqiuzi'had the strongest drought resistance,and‘Qingzhen 1'and‘B.9'were also relatively droughtresistant.These rootstocks can be used as raw materials for drought-resistant apple rootstock breeding and are propagated and utilized in arid areas.
基金Supported by University Research Project of Education Dpartment(2018A-035)~~
摘要[Objective] Iron deficiency is one of the most important crop element deficiencies in the Loess Plateau of northwestern China. The selection for crop cultivars that are tolerant to low iron levels could be one of the approaches to solving the problem and improving crop production. [Method] Three major apple root stock species (Malus prunifolia, Malus sieversii and Malus baccata) were selected to evaluate their tolerance to iron defciency in hydroponic system. A 3×2 factorial pot experiment was conducted with three replicates in a greenhouse at Gansu Agricultural University, Lanzhou, China. [Result] The SOD, POD and CAT activities in roots and stems of the 3 root stock species in Fe-defcient Hoagland solution decreased, however Malus sieversii got the less reduction and had better root architecture and growth than the other species. The aboveground biomass, plant height, chlorophyll content, total root length and lateral root number were correlated positively with iron-defciency stress tolerance. The species’ tolerance to iron-defciency from high to low was M. sieversii’s〉M. baccata’s〉M. prunifolia’s. Moreover, the improvement of some morphological features such as root length, above-ground biomass, plant height and lateral root number in apple could be conducive to breeding cultivars with tolerance to iron-defciency stress. [Conclusion] Malus sieversii had better tolerance to iron-defciency stress than the others in this study.
基金Supported by National Apple Industry Programs of Ministry of Agriculture(CARS-28)~~
摘要[Ohjective] The aim of the study is to investigate the productivity and light quality in two high density M26 dwarf rootstock apple orchards in central China so as to provide some management guidance for close planting of dwarf rootstock apples.[Method] The technical parameters of individual trees and group parameters as shoot number and composition and canopy coverage were determined, and the light quality in the canopy, fruit production and quality were investigated. [Result] Slender spindle (SS) orchard has 54 thousands shoots per 667 m^2. Coverage rate is 76%. Leaf area index is 1.9. The ratio of long, medium and spur shoots is 1:1:8. Fruit yield is 3 263 kg/667 m^2 with 85% first grade fruit. Light interception in the canopy is 58% while the ratio of canopy with good light is 65%. Modified slender spindle (MSS) orchard has 93 thousands shoots per 667 m^2 and the coverage is 77%. Leaf area index is 3.3. The ratio cf long, medium and spur shoots is 1:2:7. Fruit yield is 3 931 kg/667 m^2 with 85% first grade fruit. The light interception in the canopy is 73% while the ratio of canopy with good light is 35%. [Conclusion] Apple orchard with M26 dwarf rootstock trained as SS and MSS tree form in medium planting density may be useful to the management of the similar orchards in Central China.
基金supported by the Independent innovation fund of agricultural science and technology in Ningxia Hui autonomous region (Grant No. NGSB-2021-7-03).
摘要Grafting is a common technique used to enhance watermelon yield under biotic and abiotic stress conditions;however,it may influence fruit development and quality.This study evaluated the impact of grafting on the fruit quality attributes and volatile profiles of'Ningnongke huadai'watermelon using 11 commercial rootstocks,encompassing wild watermelons,bottle gourds,and pumpkins.Significant variations were observed in fruit weight,length,rind thickness,firmness of rind and flesh,and central soluble solids content.Notably,wild watermelon grafting increased central soluble solids,while pumpkin-grafted fruits exhibited higher fruit weight but lower central soluble solids.The analysis identified 122 volatile compounds in watermelon samples,primarily ketones,aldehydes,and alcohols.Regarding volatile composition,bottle gourd and wild watermelon grafts did not significantly differ from non-grafted watermelons,whereas pumpkin-grafted fruits showed distinctive volatile profiles characterized by higher aldehyde content and lower levels of alcohols and aromatic hydrocarbons.In conclusion,wild watermelon rootstocks increased soluble solids and had minimal impact on the volatile profiles of grafted fruits,making them potentially suitable for commercial production of'Ningnongke huadai'watermelon.The evident variability in volatile profiles among grafting combinations underscores the need for watermelon rootstock breeding programs to account for the influence of rootstocks on fruit aroma.
基金supported by the earmarked fund for the China Agriculture Research System (CARS-27)
摘要Soil alkalinity is a major factor that restricts the growth of apple roots.To analyze the response of apple roots to alkali stress, the root structure and endogenous hormones of two apple rootstocks, Malus prunifolia (alkali-tolerant) and Malus hupehensis (alkali-sensitive), were compared. To understand alkali tolerance of M. prunifolia at the molecular level, transcriptome analysis was performed. When plants were cultured in alkaline conditions for 15 d, the root growth of M. hupehensis with weak alkali tolerance decreased significantly. Analysis of endogenous hormone levels showed that the concentrations of indole-3-acetic acid (IAA) and zeatin riboside (ZR) in M. hupehensis under alkali stress were lower than those in the control. However, the trend for IAA and ZR in M. prunifolia was the opposite. The concentration of abscisic acid (ABA) in the roots of the two apple rootstocks under alkali stress increased, but the concentration of ABA in the roots of M. prunifolia was higher than that in M. hupehensis. The expression of IAA-related genes ARF5, GH3.6, SAUR36, and SAUR32 and the Cytokinin (CTK)-related gene IPT5 in M. prunifolia was higher than those in the control, but the expression of these genes in M. hupehensis was lower than those in the control. The expression of ABA-related genes CIPK1 and AHK1 increased in the two apple rootstocks under alkali stress, but the expression of CIPK1 and AHK1 in M. prunifolia was higher than in M. hupehensis. These results demonstrated that under alkali stress, the increase of IAA, ZR, and ABA in roots and the increase of the expression of related genes promoted the growth of roots and improved the alkali tolerance of apple rootstocks.
基金financially supported by the National Key R&D Program of China (2018YFD1000101 and2018YFD0201503)the earmarked fund for China Agriculture Research System (CARS-26)+3 种基金the Fundamental Research Funds for the Central Universities,China(XDJK2016B024)the National Citrus Engineering Research Center,China (NCERC)the earmarked fund for Chongqing Special&Economic Agriculture Research System on Late Maturation Citrus,Chinathe Basic Research and Frontier Exploration Projects in Chongqing,China(cstc2018jcyjAX0400)。
摘要Selection of rootstock is very important for citrus production. Besides its major role on resistance, the rootstock also can affect fruit production and quality. Currently, the main concerns on selection of rootstock for citrus production are compatibility and resistance, due to less information on the impacts of rootstock to the performance of scion varieties. This study aims to provide information on performances of navel orange varieties on different rootstocks. Three late-ripening navel orange varieties(Citrus sinensis var. Powell, Chislett and Banfield) grafted on seven rootstocks(Swingle citrumelo(C. paradisi× P. trifoliata), Carrizo citrange(C. sinensis×P. trifoliata), X639(C. reticulata×P. trifoliata), MXT(C. sinensis×P. trifoliata), Hongju(C. reticulata), Ziyang Xiangcheng(C. junos) and trifoliate orange(P. trifoliata)) were used as plant materials for comprehensive comparison of the performances on tree growth, fruit yield and quality in 21 scion–stock combinations. Investigation was carried out in these combinations in field nine years after planting. Vigorous growth of all the three late-ripening navel orange varieties was observed on Carrizo citrange with the largest canopy volume at 33.34 m3 and the highest yield at 29.43 kg per tree, but a low yield efficiency at 2.87 kg m–3. On the contrary, those on trifoliate orange had the smallest canopy volume at 10.79 m3 and the lowest fruit yield at 12.51 kg per tree, but the highest yield efficiency at 3.95 kg m–3. Rootstocks did not show significant effects on fruit size, fruit shape index, peel thickness and the edible rate of the fruits, but fruit quality was significantly affected by the rootstocks. Fruits from the trees grafted on trifoliate orange presented the best quality with significantly higher total soluble solids(TSS) content than those on Ziyang Xiangcheng and Hongju, and also the highest ratio of TSSitratable acidity(TA). The TA content was observed from the fruits on X639 at 0.59 g 100 mL–1. Vitamin C(Vc) content of fruits on Hongju was the highest at 49.25 mg 100 mL–1. Growth vigor of the trees was positively correlated with fruit yield at an extremely significant level. The canopy volume was negatively correlated with yield efficiency, but positively correlated with compatibility index. Results of this study indicated that the rootstock has great impacts on the growth vigor of the tree, yield efficiency and quality of the fruit. In order to achieve good quality and yield efficiency for navel orange production, less growth vigor rootstock such as trifoliate orange is highly recommended.