Thinopyrum ponticum(2n=10×=70),a wild relative of common wheat(Triticum aestivum L.),is considered an invaluable genetic resource for wheat improvement due to its abundance of genes conferring resistance to bioti...Thinopyrum ponticum(2n=10×=70),a wild relative of common wheat(Triticum aestivum L.),is considered an invaluable genetic resource for wheat improvement due to its abundance of genes conferring resistance to biotic and abiotic stresses.This study focused on the CH97 line,derived from the BC1F7 progeny of a cross between wheat cv.7182 and Th.ponticum.Cytological evidence showed that CH97 has 42 chromosomes,forming 21 bivalents at meiotic metaphase I,with the bivalents subsequently separating and moving to opposite poles during meiotic anaphase I.Through a combination of fluorescence in situ hybridization(FISH),genomic in situ hybridization(GISH),multicolor GISH(mc-GISH),and liquid array analysis,it was determined that CH97 comprises 40 wheat chromosomes and two alien chromosomes from the Ee genome of Th.ponticum,featuring the absence of a pair of 5D chromosomes and variations in 1B,6B,and 7B chromosomes.These findings confirm that CH97 is a stable wheat-Th.ponticum 5E(5D)alien disomic substitution line.Inoculation experiments revealed that CH97 exhibits high resistance to wheat powdery mildew and stripe rust throughout the growth period,in contrast to the highly susceptible common wheat parent 7182.Compared to 7182,CH97 displayed improvements in thousand-kernel weight and kernel length.Additionally,utilizing specific-locus amplified fragment sequencing(SLAF-seq)technology,chromosome 5E-specific molecular markers were developed and validated,achieving a 33.3% success rate,facilitating marker-assisted selection for disease resistance in wheat.Overall,the CH97 substitution line,with its resistance to diseases and improved agronomic traits,represents valuable new germplasm for wheat chromosome engineering and breeding.展开更多
The corrosion protection mechanism of a Cr-Ni-Cu-Mo multi-alloyed weathering steel(Q500qENH)is systematically investigated by coupling experimental characterization with dissolution-diffusion-deposition modeling.Compa...The corrosion protection mechanism of a Cr-Ni-Cu-Mo multi-alloyed weathering steel(Q500qENH)is systematically investigated by coupling experimental characterization with dissolution-diffusion-deposition modeling.Compared with conventional Q500q steel,Q500qENH steel exhibits one order of magnitude lower metal-ion concentration in the electrolyte,effectively alleviating acidification caused by hydrolysis and retarding substrate dissolution.The rust layer evolves through sequential deposition of Fe3O4,MoO2,Cr2O3,and CuO,forming a dense and defect-minimized microstructure.Thermodynamic and kinetic analyses reveal that the nucleation rates of Fe3O4 and CuO in Q500qENH steel are two orders of magnitude higher than in Q500q steel,accelerating the establishment of a compact barrier film.The multi-alloy synergy enhances α-FeOOH and FeCr2O4 formation,increasing charge-transfer resistance(Rct)and polarization resistance(Rp)over exposure time.These results demonstrate that Cr,Mo,and Cu collectively improve ion equilibrium and oxide nucleation behavior,offering a quantitative understanding of rust layer evolution and superior long-term corrosion protection in multi-alloyed weathering steels.展开更多
Aegilops mutica(genome T)harbors stripe rust resistance and is a potentially valuable genetic resource for broadening the genetic basis of disease resistance in common wheat.A dominant Ph1-suppression system in Ae.mut...Aegilops mutica(genome T)harbors stripe rust resistance and is a potentially valuable genetic resource for broadening the genetic basis of disease resistance in common wheat.A dominant Ph1-suppression system in Ae.mutica promotes homoeologous pairing and recombination between wheat and alien chromosomes even in the presence of Ph1,providing a tool for alien gene mapping and introgression.In this study,we identified an all-stage stripe rust resistance gene,Yr1T,in Ae.mutica and mapped it to chromosome 1T using chromosome deletion lines of Langdon×Ae.mutica amphidiploids.Genetic stocks with an AABBTD genomic constitution were generated by crossing resistant amphidiploids with the susceptible common wheat cultivar MX169.We induced homoeologous recombination between chromosome 1T and wheat homoeologous group 1 chromosomes by leveraging the Ae.mutica Ph1-suppression system.Integrating cytological identification,molecular markers,and RNA-seq breakpoint mapping,Yr1T was delimited to an approximately 20 Mb physical interval near the centromere in chromosome arm 1TS.Screening of a 100-plant F2 population yielded six recombinant plants with different breakpoints(6%recombination frequency),and a translocation line carrying Yr1T was developed.Collectively,these results demonstrated the high efficiency of the Ae.mutica Ph1-suppression system for alien gene mapping and targeted transfer,providing a practical reference for its application in wheat breeding.展开更多
Waterborne acrylic coatings are widely utilized due to their cost-effectiveness,high transparency,strong resistance to weather and chemicals,impressive mechanical properties,and excellent adhesion to various substrate...Waterborne acrylic coatings are widely utilized due to their cost-effectiveness,high transparency,strong resistance to weather and chemicals,impressive mechanical properties,and excellent adhesion to various substrates.In these coatings,a reactive emulsifier containing phosphate groups can be integrated into the molecular chain during polymerization,which enhances the coating's compactness and corrosion resistance.This work focuses on the synthesis of styrene-butyl acrylate(St-BA)latex and methyl methacrylate-butyl acrylate(MMA-BA)latex using the reactive phosphate emulsifier ANPEO10-P1through seed emulsion polymerization,achieving a conversion rate of approximately 99%and a solid content close to 50%.The resulting coatings from St-BA and MMA-BA latexes demonstrated long-term corrosion protection for carbon steel and aluminum alloy due to in-situ phosphatization,effectively preventing flash rust.Notably,the MMA-BA coating exhibited remarkable durability,enduring immersion for up to 1224 h(51 d)on Q 235 carbon steel before reaching the failure threshold(|Z|_(0.01 Hz)≤106Ω·cm2)on Q 235 carbon steel.On 5052 aluminum alloy,the St-BA coating maintained|Z|_(0.01 Hz)>108Ω·cm2for 480 h(20 d).Furthermore,the corrosion resistance of St-BA and MMA BA coatings on Q 235 steel sheet and 5052 aluminum alloy surpassed that of commercially available MMA-BA and St BA coatings after immersion in a 3.5 wt%NaCl aqueous solution.This work also delves into the anticorrosion mechanism of MMA-BA and St-BA coatings.展开更多
Healthcare,education,technological advancement,and farming are the key challenges facing developing countries,with agriculture unquestionably playing an important role in economic growth.Ensuring adequate food product...Healthcare,education,technological advancement,and farming are the key challenges facing developing countries,with agriculture unquestionably playing an important role in economic growth.Ensuring adequate food production is essential for citizens’survival,as it is anticipated that efforts in this area would result in increased food productivity.A key approach to enhancing field productivity involves meticulous care of its components,starting with the production of crops.Wheat leaf rust poses a severe threat,particularly to young seedlings,constituting a significant fungal disease that can cause a 25%reduction in wheat productivity.To overcome these issues,this research work proposes a novel image fusion approach called Multi-Scale Discrete Wavelet Transform(MS-DWT).The method uses distinct fusion strategies to extract meaningful details from source images.After that,a Lab Color Space(LCS),followed by a color thresholding method,is employed for the detection and lesion localization of rust in the source images.Furthermore,the proposed model measures the area affected by rust in wheat crops,providing farmers with vital information during the post-medication(anti-rust spray)operation.The experimental findings demonstrate superior performance and achieve a classification accuracy of 98.85%,and the maximum testing accuracy was 99.17%on our generated dataset.展开更多
Wheat leaf rust,caused by Puccinia triticina(Pt),is one of the most devastating diseases in common wheat(Triticum aestivum L.)and can lead to heavy yield loss(Chai et al.2020).Leaf rust can result in 50%yield loss dur...Wheat leaf rust,caused by Puccinia triticina(Pt),is one of the most devastating diseases in common wheat(Triticum aestivum L.)and can lead to heavy yield loss(Chai et al.2020).Leaf rust can result in 50%yield loss during epidemic years(Huerta-Espino et al.2011;Gebrewahid et al.2020;Kolomiets et al.2021).Breeding varieties resistant to leaf rust have been recognized as the most effective and economical method to mitigate wheat losses caused by Pt.The narrow genetic basis of wheat constrains the number of cultivars resistant to leaf rust(Jin et al.2021).展开更多
Aegilops variabilis(SvSvUvUv)is a source of resistance to wheat stripe rust.The phKL locus in Chinese common wheat landrace Kaixian-Luohanmai(KL)can induce homoeologous wheat-alien chromosome pairing and r...Aegilops variabilis(SvSvUvUv)is a source of resistance to wheat stripe rust.The phKL locus in Chinese common wheat landrace Kaixian-Luohanmai(KL)can induce homoeologous wheat-alien chromosome pairing and recombination.In this study,we confirmed that the whole 2Svchromosome introgressed into wheat from Ae.variabilis accession AS116 conferred all-stage stripe rust resistance.The underlying gene(s),named YrAev,was mapped to the long arm 2SvL using an F2population.Two 2Sv-2B recombinants,derived from a cross of the 2Sv(2B)chromosome substitution line and KL,were confirmed to harbor the resistance locus.The physical region containing YrAev,determined from RNA-seq data,was 844.6-852.1 Mb on the chromosome arm 2Slof the Ae.longissima(Svgenome donor species of Ae.variabilis)accession TL05 assembly v1.0.Differential gene expression analysis of post-inoculation with the Pst race has indicated two disease-resistance-related genes(annotated as mixed lineage kinase domain-like protein and nucleotide-binding leucine-rich repeat like protein,respectively)as promising candidates for YrAev.This study demonstrates the utility of the phKL gene system in alien gene localization and transfer.The resistant translocation line harboring YrAev can be exploited by wheat breeders as a novel source of resistance to stripe rust.展开更多
Southern corn rust(SCR)is an airborne fungal disease caused by Puccinia polysora Underw.(P.polysora)that adversely impacts maize quality and yields worldwide.Screening for new elite SCR-resistant maize loci or genes h...Southern corn rust(SCR)is an airborne fungal disease caused by Puccinia polysora Underw.(P.polysora)that adversely impacts maize quality and yields worldwide.Screening for new elite SCR-resistant maize loci or genes has the potential to enhance overall resistance to this pathogen.Using phenotypic SCR resistance-related data collected over two years and three geographical environments,a genome-wide association study was carried out in this work,which eventually identified 91 loci that were substantially correlated with SCR susceptibility.These included 13 loci that were significant in at least three environments and overlapped with 74 candidate genes(B73_RefGen_v4).Comparative transcriptomic analyses were then performed to identify the genes related to SCR infection,with 2,586 and 797 differentially expressed genes(DEGs)ultimately being identified in the resistant Qi319and susceptible 8112 inbred lines following P.polysora infection,respectively,including 306 genes common to both lines.Subsequent integrative multi-omics investigations identified four potential candidate SCR response-related genes.One of these genes is ZmHCT9,which encodes the protein hydroxycinnamoyl transferase 9.This gene was up-regulated in susceptible inbred lines and linked to greater P.polysora resistance as confirmed through cucumber mosaic virus(CMV)-based virus induced-gene silencing(VIGS)system-mediated gene silencing.These data provide important insights into the genetic basis of the maize SCR response.They will be useful for for future research on potential genes related to SCR resistance in maize.展开更多
The rust layer is a critical factor in determining the corrosion resistance performance of weathering bridge steel.Understanding the evolution mechanism of this rust layer is fundamental for the design and optimizatio...The rust layer is a critical factor in determining the corrosion resistance performance of weathering bridge steel.Understanding the evolution mechanism of this rust layer is fundamental for the design and optimization of such steel.This study investigates the evolu-tion of the rust layer on high-Cr-content weathering bridge steel,using an atmospheric corrosion monitoring(ACM)sensor and big data mining techniques in a simulated tropical marine atmosphere.Results reveal that the protective properties of the rust layer follow a peri-odic pattern of“ascending–constant”rather than a continuous ascending.Correlation analysis indicates that this phenomenon is attributed to the introduction of Cr,which promotes the formation of FeCr2O4 in the rust layer.FeCr2O4 helps prevent chloride ions from penetrating the rust layer,exerting a protective effect.These findings provide a strong scientific foundation for the design and improvement of new high-Cr-content weathering bridge steels.展开更多
A micro-layered structure was observed in the stable rust layer through dry–wet cycle corrosion tests.The microstructure,element distribution,and corrosion resistance mechanism of this layer structure in the marine i...A micro-layered structure was observed in the stable rust layer through dry–wet cycle corrosion tests.The microstructure,element distribution,and corrosion resistance mechanism of this layer structure in the marine industrial atmosphere were investigated by focused ion beam–scanning electron microscopy and high-resolution transmission electron microscopy.The inner rust layer of weathering steel(WS)exhibited layered enrichment of Cr,Cu,Ti,and other alloying elements.This layered structure comprised a fully amorphous region rich in Cr and Ti,as well as an amorphous and nanocrystalline region rich in Fe but poor in Cr.Only the completely amorphous region of the inner rust layer demonstrated a strong adsorption effect on Cl−.Cr-rich amorphous region played a pivotal role in reducing the corrosion of WS in the marine industrial atmospheric environment.The interface between the amorphous and nanocrystalline regions,as well as cracks within the interlayer,facilitated Cl−transport,while the intact interlayer effectively hindered HSO3−.展开更多
Panax ginseng is a traditional herbal medicine used worldwide.Rust rot caused by Cylindrocarpon destructans infects the root of P.ginseng and usually results in severe yield losses and quality deterioration.Quality co...Panax ginseng is a traditional herbal medicine used worldwide.Rust rot caused by Cylindrocarpon destructans infects the root of P.ginseng and usually results in severe yield losses and quality deterioration.Quality control of P.ginseng is crucial to ensure its medicinal value.However,the increasing application of chemical fungicides for controlling severe epidemics of diseases including rust rot in ginseng-producing regions every year has led to significant environmental pollution,fungicide residues in harvested ginseng,and an increased risk of phytopathogen resistance.In this study,an endophytic Bacillus subtillis strain,temporarily named ge28,which has shown strong antagonistic activity to C.destructans,was isolated from healthy roots of P.ginseng.In vitro and pot experiments showed fermentation and the supernatant of ge28 exhibited significant biocontrol efficacy on rust rot.The whole genome of ge28 was 4,065,429 bp,containing 4,162 functional genes.Furthermore,three clusters of non-ribosomal peptide synthetase genes conferring the synthesis of lipopeptides,a group of secondary metabolites exhibiting antagonistic properties,including surfactin,fengycin,and bacillibactin,were identified through genome analysis.Surfactin and fengycin were confirmed to be present in the supernatant of ge28 by UPLC-ESI-QTOF-MS analysis.In conclusion,this study demonstrated that the ge28 strain possesses substantial biocontrol potential against the rust rot of P.ginseng,and its antagonistic mechanisms were preliminarily clarified.These results should accelerate the utilization of ge28 to enhance the yield and quality of P.ginseng through reduced fungicide application.展开更多
REST(Rust-based electronic structure toolkit)is a modern open-source electronic structure code entirely written in Rust,combining high performance,memory safety,and expressive concurrency.As a community-driven project...REST(Rust-based electronic structure toolkit)is a modern open-source electronic structure code entirely written in Rust,combining high performance,memory safety,and expressive concurrency.As a community-driven project,its source code is freely available at http://gffzz86db22e41f624027sofbb0pu5xcxk6xbx.ffgz.tsg.suse.edu.cn,fostering open collaboration and transparent development.It supports a wide range of density functional methods-from local density approximation(LDA),generalized gradient approximation(GGA),meta-GGA,and hybrids to doubly hybrids,as well as machine learning-augmented functionals-enabling high-accuracy simulations with low computational overhead.Its“disk-free”RI-based(RI:resolution-of-the-identity)implementation and efficient shared-memory parallelism(via Rayon)ensure rapid calculations even for challenging systems.REST also of-fers unique user support through large language model-assisted input generation and develop-erfriendly tensor libraries for rapid algorithm prototyping.展开更多
Yellow rust(Puccinia striiformis f.sp.Tritici,YR)and fusarium head blight(Fusarium graminearum,FHB)are the two main diseases affecting wheat in the main grain-producing areas of East China,which is common for the two ...Yellow rust(Puccinia striiformis f.sp.Tritici,YR)and fusarium head blight(Fusarium graminearum,FHB)are the two main diseases affecting wheat in the main grain-producing areas of East China,which is common for the two diseases to appear simultaneously in some main production areas.It is necessary to discriminate wheat YR and FHB at the regional scale to accurately locate the disease in space,conduct detailed disease severity monitoring,and scientific control.Four images on different dates were acquired from Sentinel-2,Landsat-8,and Gaofen-1 during the critical period of winter wheat,and 22 remote sensing features that characterize the wheat growth status were then calculated.Meanwhile,6 meteorological parameters that reflect the wheat phenological information were also obtained by combining the site meteorological data and spatial interpolation technology.Then,the principal components(PCs)of comprehensive remote sensing and meteorological features were extracted with principal component analysis(PCA).The PCs-based discrimination models were established to map YR and FHB damage using the random forest(RF)and backpropagation neural network(BPNN).The models’performance was verified based on the disease field truth data(57 plots during the filling period)and 5-fold cross-validation.The results revealed that the PCs obtained after PCA dimensionality reduction outperformed the initial features(IFs)from remote sensing and meteorology in discriminating between the two diseases.Compared to the IFs,the average area under the curve for both micro-average and macro-average ROC curves increased by 0.07 in the PCs-based RF models and increased by 0.16 and 0.13,respectively,in the PCs-based BPNN models.Notably,the PCs-based BPNN discrimination model emerged as the most effective,achieving an overall accuracy of 83.9%.Our proposed discrimination model for wheat YR and FHB,coupled with multi-source remote sensing images and meteorological data,overcomes the limitations of a single-sensor and single-phase remote sensing information in multiple stress discrimination in cloudy and rainy areas.It performs well in revealing the damage spatial distribution of the two diseases at a regional scale,providing a basis for detailed disease severity monitoring,and scientific prevention and control.展开更多
Frame blades were used to replace traditional propeller blades to enhance the leaching step efficiency of Becher process.A combined approach of leaching,electrochemical experiments,and numerical simulations was employ...Frame blades were used to replace traditional propeller blades to enhance the leaching step efficiency of Becher process.A combined approach of leaching,electrochemical experiments,and numerical simulations was employed.Results demonstrate a significant improvement in leaching efficiency using frame blades compared to propellers,reducing reaction time from 15 to 10 h.Even at a stirring speed of 300 r/min,frame blades perform better than propellers at 500 r/min.Kinetics analysis indicates that the leaching process is controlled by surface chemical reactions.CFD-PBM simulations reveal that frame blades at 300 r/min generate larger bubbles and higher turbulent kinetic energy than propeller blades at 500 r/min.Frame blades enhance leaching efficiency by refining bubble size to improve oxygen mass transfer and by increasing turbulent kinetic energy for better mixing.展开更多
基金funded by the Key R&D Program of Yangling Seed Industry Innovation,China(Ylzy-xm-02)the Young Elite Scientists Sponsorship Program by China Association for Science and Technology(2021QNRC001)。
摘要Thinopyrum ponticum(2n=10×=70),a wild relative of common wheat(Triticum aestivum L.),is considered an invaluable genetic resource for wheat improvement due to its abundance of genes conferring resistance to biotic and abiotic stresses.This study focused on the CH97 line,derived from the BC1F7 progeny of a cross between wheat cv.7182 and Th.ponticum.Cytological evidence showed that CH97 has 42 chromosomes,forming 21 bivalents at meiotic metaphase I,with the bivalents subsequently separating and moving to opposite poles during meiotic anaphase I.Through a combination of fluorescence in situ hybridization(FISH),genomic in situ hybridization(GISH),multicolor GISH(mc-GISH),and liquid array analysis,it was determined that CH97 comprises 40 wheat chromosomes and two alien chromosomes from the Ee genome of Th.ponticum,featuring the absence of a pair of 5D chromosomes and variations in 1B,6B,and 7B chromosomes.These findings confirm that CH97 is a stable wheat-Th.ponticum 5E(5D)alien disomic substitution line.Inoculation experiments revealed that CH97 exhibits high resistance to wheat powdery mildew and stripe rust throughout the growth period,in contrast to the highly susceptible common wheat parent 7182.Compared to 7182,CH97 displayed improvements in thousand-kernel weight and kernel length.Additionally,utilizing specific-locus amplified fragment sequencing(SLAF-seq)technology,chromosome 5E-specific molecular markers were developed and validated,achieving a 33.3% success rate,facilitating marker-assisted selection for disease resistance in wheat.Overall,the CH97 substitution line,with its resistance to diseases and improved agronomic traits,represents valuable new germplasm for wheat chromosome engineering and breeding.
基金the National Natural Science Foundation of China(Nos.52374323 and 52522409).
摘要The corrosion protection mechanism of a Cr-Ni-Cu-Mo multi-alloyed weathering steel(Q500qENH)is systematically investigated by coupling experimental characterization with dissolution-diffusion-deposition modeling.Compared with conventional Q500q steel,Q500qENH steel exhibits one order of magnitude lower metal-ion concentration in the electrolyte,effectively alleviating acidification caused by hydrolysis and retarding substrate dissolution.The rust layer evolves through sequential deposition of Fe3O4,MoO2,Cr2O3,and CuO,forming a dense and defect-minimized microstructure.Thermodynamic and kinetic analyses reveal that the nucleation rates of Fe3O4 and CuO in Q500qENH steel are two orders of magnitude higher than in Q500q steel,accelerating the establishment of a compact barrier film.The multi-alloy synergy enhances α-FeOOH and FeCr2O4 formation,increasing charge-transfer resistance(Rct)and polarization resistance(Rp)over exposure time.These results demonstrate that Cr,Mo,and Cu collectively improve ion equilibrium and oxide nucleation behavior,offering a quantitative understanding of rust layer evolution and superior long-term corrosion protection in multi-alloyed weathering steels.
基金supported by the National Key Research and Development Program of China(2024YFD1201202)Sichuan Science and Technology Program(2021YFYZ0002)Sichuan Provincial Agricultural Department Innovative Research Team(SCCXTD2024-11)。
摘要Aegilops mutica(genome T)harbors stripe rust resistance and is a potentially valuable genetic resource for broadening the genetic basis of disease resistance in common wheat.A dominant Ph1-suppression system in Ae.mutica promotes homoeologous pairing and recombination between wheat and alien chromosomes even in the presence of Ph1,providing a tool for alien gene mapping and introgression.In this study,we identified an all-stage stripe rust resistance gene,Yr1T,in Ae.mutica and mapped it to chromosome 1T using chromosome deletion lines of Langdon×Ae.mutica amphidiploids.Genetic stocks with an AABBTD genomic constitution were generated by crossing resistant amphidiploids with the susceptible common wheat cultivar MX169.We induced homoeologous recombination between chromosome 1T and wheat homoeologous group 1 chromosomes by leveraging the Ae.mutica Ph1-suppression system.Integrating cytological identification,molecular markers,and RNA-seq breakpoint mapping,Yr1T was delimited to an approximately 20 Mb physical interval near the centromere in chromosome arm 1TS.Screening of a 100-plant F2 population yielded six recombinant plants with different breakpoints(6%recombination frequency),and a translocation line carrying Yr1T was developed.Collectively,these results demonstrated the high efficiency of the Ae.mutica Ph1-suppression system for alien gene mapping and targeted transfer,providing a practical reference for its application in wheat breeding.
基金Project(52373065)supported by the National Natural Science Foundation of ChinaProject(2220004002898)supported by the Zhuhai Industry University Research Cooperation and Basic and Applied Research Projects,China。
摘要Waterborne acrylic coatings are widely utilized due to their cost-effectiveness,high transparency,strong resistance to weather and chemicals,impressive mechanical properties,and excellent adhesion to various substrates.In these coatings,a reactive emulsifier containing phosphate groups can be integrated into the molecular chain during polymerization,which enhances the coating's compactness and corrosion resistance.This work focuses on the synthesis of styrene-butyl acrylate(St-BA)latex and methyl methacrylate-butyl acrylate(MMA-BA)latex using the reactive phosphate emulsifier ANPEO10-P1through seed emulsion polymerization,achieving a conversion rate of approximately 99%and a solid content close to 50%.The resulting coatings from St-BA and MMA-BA latexes demonstrated long-term corrosion protection for carbon steel and aluminum alloy due to in-situ phosphatization,effectively preventing flash rust.Notably,the MMA-BA coating exhibited remarkable durability,enduring immersion for up to 1224 h(51 d)on Q 235 carbon steel before reaching the failure threshold(|Z|_(0.01 Hz)≤106Ω·cm2)on Q 235 carbon steel.On 5052 aluminum alloy,the St-BA coating maintained|Z|_(0.01 Hz)>108Ω·cm2for 480 h(20 d).Furthermore,the corrosion resistance of St-BA and MMA BA coatings on Q 235 steel sheet and 5052 aluminum alloy surpassed that of commercially available MMA-BA and St BA coatings after immersion in a 3.5 wt%NaCl aqueous solution.This work also delves into the anticorrosion mechanism of MMA-BA and St-BA coatings.
基金Prince Sattam bin Abdulaziz University for funding this research work through the project number(PSAU/202503/34113).
摘要Healthcare,education,technological advancement,and farming are the key challenges facing developing countries,with agriculture unquestionably playing an important role in economic growth.Ensuring adequate food production is essential for citizens’survival,as it is anticipated that efforts in this area would result in increased food productivity.A key approach to enhancing field productivity involves meticulous care of its components,starting with the production of crops.Wheat leaf rust poses a severe threat,particularly to young seedlings,constituting a significant fungal disease that can cause a 25%reduction in wheat productivity.To overcome these issues,this research work proposes a novel image fusion approach called Multi-Scale Discrete Wavelet Transform(MS-DWT).The method uses distinct fusion strategies to extract meaningful details from source images.After that,a Lab Color Space(LCS),followed by a color thresholding method,is employed for the detection and lesion localization of rust in the source images.Furthermore,the proposed model measures the area affected by rust in wheat crops,providing farmers with vital information during the post-medication(anti-rust spray)operation.The experimental findings demonstrate superior performance and achieve a classification accuracy of 98.85%,and the maximum testing accuracy was 99.17%on our generated dataset.
基金funded by the National Natural Science Foundation of China(32272083)。
摘要Wheat leaf rust,caused by Puccinia triticina(Pt),is one of the most devastating diseases in common wheat(Triticum aestivum L.)and can lead to heavy yield loss(Chai et al.2020).Leaf rust can result in 50%yield loss during epidemic years(Huerta-Espino et al.2011;Gebrewahid et al.2020;Kolomiets et al.2021).Breeding varieties resistant to leaf rust have been recognized as the most effective and economical method to mitigate wheat losses caused by Pt.The narrow genetic basis of wheat constrains the number of cultivars resistant to leaf rust(Jin et al.2021).
基金supported by the National Natural Science Foundation of China(32172020,31971884)the National Key Research and Development Program of China(2024YFD1201202,2024YFD1200402)+4 种基金the Sichuan Science and Technology Program(2022ZDZX0014,2023YFN0085)the Qinghai Provincial Key Laboratory of Crop Molecular Breeding(2023-1-1)the State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China(SKLKF202409)the Alliance of National and International Science Organizations for the Belt and Road Regions(ANSO-CR-KP-202205)the International Partnership Program of Chinese Academy of Sciences(077GJHZ2023028GC)。
摘要Aegilops variabilis(SvSvUvUv)is a source of resistance to wheat stripe rust.The phKL locus in Chinese common wheat landrace Kaixian-Luohanmai(KL)can induce homoeologous wheat-alien chromosome pairing and recombination.In this study,we confirmed that the whole 2Svchromosome introgressed into wheat from Ae.variabilis accession AS116 conferred all-stage stripe rust resistance.The underlying gene(s),named YrAev,was mapped to the long arm 2SvL using an F2population.Two 2Sv-2B recombinants,derived from a cross of the 2Sv(2B)chromosome substitution line and KL,were confirmed to harbor the resistance locus.The physical region containing YrAev,determined from RNA-seq data,was 844.6-852.1 Mb on the chromosome arm 2Slof the Ae.longissima(Svgenome donor species of Ae.variabilis)accession TL05 assembly v1.0.Differential gene expression analysis of post-inoculation with the Pst race has indicated two disease-resistance-related genes(annotated as mixed lineage kinase domain-like protein and nucleotide-binding leucine-rich repeat like protein,respectively)as promising candidates for YrAev.This study demonstrates the utility of the phKL gene system in alien gene localization and transfer.The resistant translocation line harboring YrAev can be exploited by wheat breeders as a novel source of resistance to stripe rust.
基金supported by the National Key R&D Program of China(2022YFD1201802)the Shandong Key R&D Program,China(2022CXGC010607)+2 种基金the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ZDRW202109)the Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ASTIP2017-ICS)the Subproject of the Major Project of Science and Technology in Shanxi Province,China(202201140601025-1-02)。
摘要Southern corn rust(SCR)is an airborne fungal disease caused by Puccinia polysora Underw.(P.polysora)that adversely impacts maize quality and yields worldwide.Screening for new elite SCR-resistant maize loci or genes has the potential to enhance overall resistance to this pathogen.Using phenotypic SCR resistance-related data collected over two years and three geographical environments,a genome-wide association study was carried out in this work,which eventually identified 91 loci that were substantially correlated with SCR susceptibility.These included 13 loci that were significant in at least three environments and overlapped with 74 candidate genes(B73_RefGen_v4).Comparative transcriptomic analyses were then performed to identify the genes related to SCR infection,with 2,586 and 797 differentially expressed genes(DEGs)ultimately being identified in the resistant Qi319and susceptible 8112 inbred lines following P.polysora infection,respectively,including 306 genes common to both lines.Subsequent integrative multi-omics investigations identified four potential candidate SCR response-related genes.One of these genes is ZmHCT9,which encodes the protein hydroxycinnamoyl transferase 9.This gene was up-regulated in susceptible inbred lines and linked to greater P.polysora resistance as confirmed through cucumber mosaic virus(CMV)-based virus induced-gene silencing(VIGS)system-mediated gene silencing.These data provide important insights into the genetic basis of the maize SCR response.They will be useful for for future research on potential genes related to SCR resistance in maize.
基金supported by the National Natural Science Foundation of China(No.52171063).
摘要The rust layer is a critical factor in determining the corrosion resistance performance of weathering bridge steel.Understanding the evolution mechanism of this rust layer is fundamental for the design and optimization of such steel.This study investigates the evolu-tion of the rust layer on high-Cr-content weathering bridge steel,using an atmospheric corrosion monitoring(ACM)sensor and big data mining techniques in a simulated tropical marine atmosphere.Results reveal that the protective properties of the rust layer follow a peri-odic pattern of“ascending–constant”rather than a continuous ascending.Correlation analysis indicates that this phenomenon is attributed to the introduction of Cr,which promotes the formation of FeCr2O4 in the rust layer.FeCr2O4 helps prevent chloride ions from penetrating the rust layer,exerting a protective effect.These findings provide a strong scientific foundation for the design and improvement of new high-Cr-content weathering bridge steels.
基金supported by the High-quality Scientific Research Cultivation Project of Bengbu University(Grant No.2021pyxm03)the Natural Science Foundation of the Higher Education Institutions of Anhui Province(Grant No.KJ2021A1118)+1 种基金the Provincial Quality Engineering Project of Anhui Universities(Grant No.2022zygzts066)the Discipline(Major)Leadership Development Program of Anhui Province(DTR2023057).
摘要A micro-layered structure was observed in the stable rust layer through dry–wet cycle corrosion tests.The microstructure,element distribution,and corrosion resistance mechanism of this layer structure in the marine industrial atmosphere were investigated by focused ion beam–scanning electron microscopy and high-resolution transmission electron microscopy.The inner rust layer of weathering steel(WS)exhibited layered enrichment of Cr,Cu,Ti,and other alloying elements.This layered structure comprised a fully amorphous region rich in Cr and Ti,as well as an amorphous and nanocrystalline region rich in Fe but poor in Cr.Only the completely amorphous region of the inner rust layer demonstrated a strong adsorption effect on Cl−.Cr-rich amorphous region played a pivotal role in reducing the corrosion of WS in the marine industrial atmospheric environment.The interface between the amorphous and nanocrystalline regions,as well as cracks within the interlayer,facilitated Cl−transport,while the intact interlayer effectively hindered HSO3−.
基金supported by the National Key R&D Program of China(2022YFC3501504)the CAMS Innovation Fund for Medical Sciences(2021-I2M-1-032)+1 种基金the National Project for Standardization of Traditional Chinese medicine(ZYBZH-C-JL-27)the Innovation Research Fund of Peking Union Medical College(1007-14).
摘要Panax ginseng is a traditional herbal medicine used worldwide.Rust rot caused by Cylindrocarpon destructans infects the root of P.ginseng and usually results in severe yield losses and quality deterioration.Quality control of P.ginseng is crucial to ensure its medicinal value.However,the increasing application of chemical fungicides for controlling severe epidemics of diseases including rust rot in ginseng-producing regions every year has led to significant environmental pollution,fungicide residues in harvested ginseng,and an increased risk of phytopathogen resistance.In this study,an endophytic Bacillus subtillis strain,temporarily named ge28,which has shown strong antagonistic activity to C.destructans,was isolated from healthy roots of P.ginseng.In vitro and pot experiments showed fermentation and the supernatant of ge28 exhibited significant biocontrol efficacy on rust rot.The whole genome of ge28 was 4,065,429 bp,containing 4,162 functional genes.Furthermore,three clusters of non-ribosomal peptide synthetase genes conferring the synthesis of lipopeptides,a group of secondary metabolites exhibiting antagonistic properties,including surfactin,fengycin,and bacillibactin,were identified through genome analysis.Surfactin and fengycin were confirmed to be present in the supernatant of ge28 by UPLC-ESI-QTOF-MS analysis.In conclusion,this study demonstrated that the ge28 strain possesses substantial biocontrol potential against the rust rot of P.ginseng,and its antagonistic mechanisms were preliminarily clarified.These results should accelerate the utilization of ge28 to enhance the yield and quality of P.ginseng through reduced fungicide application.
基金supported by the National Natural Science Foundation of China (Nos. 22125301, 22393911, 22393912, 22321003, 22233002)the Innovation Program for Quantum Science and Technology (2021ZD0303305)the robotic AI-Scientist platform of the Chinese Academy of Science。
摘要REST(Rust-based electronic structure toolkit)is a modern open-source electronic structure code entirely written in Rust,combining high performance,memory safety,and expressive concurrency.As a community-driven project,its source code is freely available at http://gffzz86db22e41f624027sofbb0pu5xcxk6xbx.ffgz.tsg.suse.edu.cn,fostering open collaboration and transparent development.It supports a wide range of density functional methods-from local density approximation(LDA),generalized gradient approximation(GGA),meta-GGA,and hybrids to doubly hybrids,as well as machine learning-augmented functionals-enabling high-accuracy simulations with low computational overhead.Its“disk-free”RI-based(RI:resolution-of-the-identity)implementation and efficient shared-memory parallelism(via Rayon)ensure rapid calculations even for challenging systems.REST also of-fers unique user support through large language model-assisted input generation and develop-erfriendly tensor libraries for rapid algorithm prototyping.
基金supported by National Key R&D Program of China(2022YFD2000100)National Natural Science Foundation of China(42401400)Zhejiang Provincial Key Research and Development Program(2023C02018).
摘要Yellow rust(Puccinia striiformis f.sp.Tritici,YR)and fusarium head blight(Fusarium graminearum,FHB)are the two main diseases affecting wheat in the main grain-producing areas of East China,which is common for the two diseases to appear simultaneously in some main production areas.It is necessary to discriminate wheat YR and FHB at the regional scale to accurately locate the disease in space,conduct detailed disease severity monitoring,and scientific control.Four images on different dates were acquired from Sentinel-2,Landsat-8,and Gaofen-1 during the critical period of winter wheat,and 22 remote sensing features that characterize the wheat growth status were then calculated.Meanwhile,6 meteorological parameters that reflect the wheat phenological information were also obtained by combining the site meteorological data and spatial interpolation technology.Then,the principal components(PCs)of comprehensive remote sensing and meteorological features were extracted with principal component analysis(PCA).The PCs-based discrimination models were established to map YR and FHB damage using the random forest(RF)and backpropagation neural network(BPNN).The models’performance was verified based on the disease field truth data(57 plots during the filling period)and 5-fold cross-validation.The results revealed that the PCs obtained after PCA dimensionality reduction outperformed the initial features(IFs)from remote sensing and meteorology in discriminating between the two diseases.Compared to the IFs,the average area under the curve for both micro-average and macro-average ROC curves increased by 0.07 in the PCs-based RF models and increased by 0.16 and 0.13,respectively,in the PCs-based BPNN models.Notably,the PCs-based BPNN discrimination model emerged as the most effective,achieving an overall accuracy of 83.9%.Our proposed discrimination model for wheat YR and FHB,coupled with multi-source remote sensing images and meteorological data,overcomes the limitations of a single-sensor and single-phase remote sensing information in multiple stress discrimination in cloudy and rainy areas.It performs well in revealing the damage spatial distribution of the two diseases at a regional scale,providing a basis for detailed disease severity monitoring,and scientific prevention and control.
基金supported by the National Natural Science Foundation of China(No.U1908225)the Cross-Integration and Collaborative Development Project of Northeastern University,China(No.N2225013).
摘要Frame blades were used to replace traditional propeller blades to enhance the leaching step efficiency of Becher process.A combined approach of leaching,electrochemical experiments,and numerical simulations was employed.Results demonstrate a significant improvement in leaching efficiency using frame blades compared to propellers,reducing reaction time from 15 to 10 h.Even at a stirring speed of 300 r/min,frame blades perform better than propellers at 500 r/min.Kinetics analysis indicates that the leaching process is controlled by surface chemical reactions.CFD-PBM simulations reveal that frame blades at 300 r/min generate larger bubbles and higher turbulent kinetic energy than propeller blades at 500 r/min.Frame blades enhance leaching efficiency by refining bubble size to improve oxygen mass transfer and by increasing turbulent kinetic energy for better mixing.