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Secretory leukocyte peptidase inhibitor as a dynamic radiotherapy response biomarker in esophageal squamous cell carcinoma 认领 引用
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作者 Hongming Deng Junyi Li +7 位作者 Lin‑Rui Gao Ning Huang Chenxi Wang Zefen Xiao Xiaobing Wang Liming Wang Ting Xiao Mei Liu 《MedScience》 SCIE CAS CSCD 2026年第2期311-328,共18页
Esophageal cancer is the seventh leading cause of cancer‑related deaths,with most cases diagnosed at locally advanced stages.Chemoradiotherapy is one of the most effective treatments for these patients.However,high ra... Esophageal cancer is the seventh leading cause of cancer‑related deaths,with most cases diagnosed at locally advanced stages.Chemoradiotherapy is one of the most effective treatments for these patients.However,high rates of recurrence persist,highlighting the need for more reliable prognostic biomarkers.In this study,we identified immune‑related prognostic genes through bulk(n=119)and single‑cell(n=60)transcriptomic analyses.We then used Western blotting,enzyme‑linked immunosorbent assay(ELISA),and immunofluorescence to preliminarily investigate the potential biological functions of the candidate biomarker.The clinical prognostic utility of the candidate biomarker was validated in an independent cohort(n=91)via ELISA of serum samples collected before and during chemoradiotherapy.The results showed that low expression of the secretory leukocyte peptidase inhibitor(SLPI)significantly correlated with tumor progression and poor prognosis.In epithelial cells,reduced SLPI expression was associated with decreased activation of differentiation‑related pathways.Furthermore,SLPI expression levels were found to influence fibroblast phenotypes,with exogenous SLPI inhibiting the NF‑κB pathway and restoring fibroblast quiescence.The results of ELISA showed that dynamic changes in serum SLPI levels during chemoradiotherapy serve as an independent prognostic factor.In conclusion,serum SLPI levels represent an easy‑to‑detect biomarker for dynamically monitoring the efficacy of chemoradiotherapy. 展开更多
关键词 esophageal squamous cell carcinoma secretory leukocyte peptidase inhibitor transcriptome radiotherapy prognosis
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Secretory autophagy in neurons:More than throwing out the trash? 认领 引用
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作者 Alexander Veh Patrick Lüningschrör 《Neural Regeneration Research》 SCIE CAS CSCD 2026年第3期1108-1109,共2页
Autophagy is well-known for delivering cargo materials to lysosomes for proteolytic digestion.Recently,autophagy has emerged as a key mechanism in unconventional protein secretion(UPS).This perspective introduces unco... Autophagy is well-known for delivering cargo materials to lysosomes for proteolytic digestion.Recently,autophagy has emerged as a key mechanism in unconventional protein secretion(UPS).This perspective introduces unconventional secretion pathways,focusing on secretory autophagy and its role in secreting protein aggregates associated with neurodegenerative disorders.We also explore additional neuronal functions of secretory autophagy beyond the release of protein aggregates.We propose autophagosomes as transport organelles that deliver cargo material directly from the endoplasmatic reticulum(ER)to the plasma membrane rather than solely to lysosomes. 展开更多
关键词 proteolytic digestionrecentlyautophagy secreting protein aggregates neurons protein aggregateswe delivering cargo materials unconventional protein secretion unconventional protein secretion ups secretory autophagy
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Proteostasis decline and endoplasmic reticulum stress in aging:Implications for cellular senescence and senescence-associated secretory phenotype regulation 认领 引用
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作者 Philippe Pihán Lisa M.Ellerby Claudio Hetz 《Neural Regeneration Research》 SCIE CAS CSCD 2026年第7期2934-2935,共2页
Aging is a universal biological process characterized by the progressive decline in cellular and tissue function,representing the main risk factor for the development of most chronic human diseases.At the cellular lev... Aging is a universal biological process characterized by the progressive decline in cellular and tissue function,representing the main risk factor for the development of most chronic human diseases.At the cellular level,one hallmark of aging is the accumulation of senescent cells—non-dividing yet metabolically active cells that adopt a unique phenotype,including the senescence-associated secretory phenotype(SASP)(Wang et al.,2024). 展开更多
关键词 proteostasis decline senescence associated secretory phenotype cellular tissue endoplasmic reticulum stress senescent cells non dividing cellular senescence aging
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Decoding the genetic basis of secretory tissues in plants 认领 引用
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作者 Yuepeng Han 《Horticulture Research》 SCIE CSCD 2025年第1期365-368,共4页
Although plant secretory tissues play important roles in host defense against herbivores and pathogens and the attraction of insect pollinators,their genetic control remains elusive.Here,it is focused that current pro... Although plant secretory tissues play important roles in host defense against herbivores and pathogens and the attraction of insect pollinators,their genetic control remains elusive.Here,it is focused that current progress has been made in the genetic regulatory mechanisms underpinning secretory tissue development in land plants.C1HDZ transcription factors(TFs)are found to play crucial roles in the regulation of internal secretory tissues in liverworts and Citrus as well as external secretory tissues in peach.C1HDZ TFs regulate secretory tissue development via synergistic interaction with AP2/ERF and MYC TFs.Thus,a set of genes are speculated to be recruited convergently for the formation of secretory tissues in land plants. 展开更多
关键词 plant secretory tissues genetic regulatory mechanisms plants genetic basis host defense secretory tissue insect pollinatorstheir regulation internal secretory tissues
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Laparoscopic sleeve gastrectomy combined with montelukast for obese patients with secretory otitis media 认领 引用
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作者 Hua-Long Qiang Xiang-Li Yao +1 位作者 Zhen-Feng Jiang Wei Wang 《World Journal of Gastrointestinal Surgery》 SCIE 2026年第6期153-160,共8页
BACKGROUND Obesity is associated with increased risk of otitis media with effusion(OME),potentially due to peritubal fat accumulation and chronic inflammation.Laparoscopic sleeve gastrectomy(LSG)effectively reduces we... BACKGROUND Obesity is associated with increased risk of otitis media with effusion(OME),potentially due to peritubal fat accumulation and chronic inflammation.Laparoscopic sleeve gastrectomy(LSG)effectively reduces weight but achieves slow early middle ear effusion resolution.Montelukast,a leukotriene receptor antagonist,possesses anti-inflammatory properties that may promote OME recovery.However,rapid weight loss may paradoxically induce patulous eustachian tube,complicating outcome interpretation.We hypothesized that combining LSG with montelukast would accelerate middle ear effusion clearance and improve eustachian tube function without compromising weight loss efficacy.AIM To evaluate LSG combined with montelukast for obese patients with OME regarding recovery,eustachian tube function,and weight loss.METHODS This retrospective cohort study enrolled 112 obese OME patients treated at a tertiary hospital between January 2020 and March 2025.Groups:Control(n=48,LSG alone)and observation(n=64,LSG plus oral montelukast 10 mg/day for one month).Outcomes included total weight loss,excess weight loss,middle ear effusion resolution time,air-bone gap,Eustachian Tube Dysfunction Questionnaire-7(ETDQ-7)score,36-Item Short Form Health Survey(SF-36),and adverse events.Assessors were blinded.Inter-group comparisons used t tests andχ2 tests.RESULTS Baseline characteristics were comparable between groups(P>0.05).Weight loss outcomes at 1 months and 6 months showed no significant inter-group differences(P>0.05).Middle ear effusion clearance was significantly faster in the observation group compared to controls(28.39±6.51 days vs 42.10±8.32 days,P0.05).CONCLUSION LSG combined with montelukast significantly accelerates middle ear effusion resolution,improves auditory function and quality of life in obese OME patients,with maintained weight loss and favorable short-term safety. 展开更多
关键词 Laparoscopic sleeve gastrectomy Montelukast Obesity Secretory otitis media Eustachian tube function Quality of life
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The Impact of Double-puncture Tympanic Membrane Puncture and Tympanic Cavity Drug Injection on the Complication Rate of Acute Secretory Otitis Media 认领 引用
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作者 Wei Zheng 《Proceedings of Anticancer Research》 2026年第1期54-60,共7页
Objective:To explore the therapeutic effect of double-puncture tympanic membrane puncture and tympanic cavity drug injection in patients with acute secretory otitis media.Methods:A total of 84 patients with acute secr... Objective:To explore the therapeutic effect of double-puncture tympanic membrane puncture and tympanic cavity drug injection in patients with acute secretory otitis media.Methods:A total of 84 patients with acute secretory otitis media admitted to our hospital from June 2024 to June 2025 were selected and randomly divided into two groups by drawing lots.The control group(42 cases)was treated with the traditional single-puncture tympanic membrane puncture and tympanic cavity drug injection method,while the observation group(42 cases)was treated with the double-puncture tympanic membrane puncture and tympanic cavity drug injection method.The therapeutic effects of the two groups were compared.Results:The overall treatment response rate,overall complication rate,time to symptom relief,and improvement in hearing threshold in the observation group were all superior to those in the control group,with statistically significant differences(P<0.05).Conclusion:For acute secretory otitis media,the treatment method of double-puncture tympanic membrane puncture and tympanic cavity drug injection demonstrates definite efficacy,significantly reducing the incidence of complications,accelerating symptom relief,and improving hearing function,making it worthy of promotion. 展开更多
关键词 Acute secretory otitis media Double puncture Tympanic membrane puncture and tympanic cavity drug injection Complications
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Sinisan,a compound Chinese herbal medicine,alleviates acute colitis by facilitating colonic secretory cell lineage commitment and mucin production 认领 引用 被引量：2
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作者 Ya-jie Cai Jian-hang Lan +4 位作者 Shuo Li Yue-ning Feng Fang-hong Li Meng-yu Guo Run-ping Liu 《Journal of Integrative Medicine》 SCIE CAS CSCD 2025年第4期429-444,共16页
Objective:Ulcerative colitis is closely associated with intestinal stem cell(ISC)loss and impaired intestinal mucus barrier.Sinisan(SNS),a compound Chinese herbal medicine,has a long history in the treatment of intest... Objective:Ulcerative colitis is closely associated with intestinal stem cell(ISC)loss and impaired intestinal mucus barrier.Sinisan(SNS),a compound Chinese herbal medicine,has a long history in the treatment of intestinal dysfunction,yet whether SNS can relieve acute experimental colitis by modulating ISC proliferation and secretory cell differentiation has not been studied.Our study tested the effect of SNS against acute colitis and focused on the mechanisms involving intestinal barrier recovery.Methods:Network pharmacology analysis and blood entry component analysis of SNS were used to explore the underlying mechanism by which SNS affects the acute dextran sulfate sodium(DSS)-induced murine colitis model.RNA-sequencing was used to demonstrate the mechanism.Further,reverse transcription-quantitative polymerase chain reaction,immunofluorescence staining,and alcian blue and periodic acid-Schiff staining were performed in vivo and in the colonic organoids to investigate the cell lineage differentiation-related mechanism of SNS.Furthermore,potential active ingredients from SNS were predicted by network pharmacology analysis.Results:SNS dramatically suppressed DSS-induced acute colonic inflammation in mice.RNA-sequencing analysis revealed downregulation of inflammation and apoptosis-related genes,and upregulation of lipid metabolism and proliferation-related genes,such as Irf7,Ppara,Clspn and Hspa5.Additionally,ISC renewal and intestinal secretory cell lineage commitment were significantly promoted by SNS both in vivo and in vitro in colonic organoids,leading to enhanced mucin expression.Furthermore,potential active ingredients from SNS that mediated inflammation,lipid metabolism,proliferation,apoptosis,stem cells and secretory cells were predicted using a network pharmacology approach.Conclusion:Our study shed light on the underlying mechanism of SNS in attenuating acute colitis from the perspective of ISC renewal and secretory lineage cell differentiation,suggesting a of novel therapeutic strategy against colitis. 展开更多
关键词 Sinisan Colitis Intestinal stem cells Secretory cell lineage
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Decorin, an exercise-induced secretory protein, is associated with improved prognosis in breast cancer patients but does not mediate anti-tumorigenic tissue crosstalk in mice 认领 引用 被引量：1
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作者 Marit Hjorth Casey L.Egan +28 位作者 Guilherme D.Telles Martin Pal David Gallego-Ortega Oliver K.Fuller Emma D.McLennan Ryan D.Gillis Tae Gyu Oh George E.O.Muscat Surafel Tegegne Michael S.M.Mah Joanna Skhinas Emma Estevez Timothy E.Adams Matthew J.McKay Mark Molloy Kevin I.Watt Hongwei Qian Paul Gregorevic Thomas R.Cox Pernille Hojman Julie Midtgaard Jesper F.Christensen Martin Friedrichsen Renato V.Iozzo Erica K.Sloan Brian G.Drew Jørgen F.P.Wojtaszewski Martin Whitham Mark A.Febbraio 《Journal of Sport and Health Science》 SCIE CSCD 2025年第3期55-72,共18页
Purpose:Regular exercise can reduce incidence and progression of breast cancer,but the mechanisms for such effects are not fully understood.The purpose of this study was to examine the mechanisms behind the protective... Purpose:Regular exercise can reduce incidence and progression of breast cancer,but the mechanisms for such effects are not fully understood.The purpose of this study was to examine the mechanisms behind the protective effects of exercise.Methods:We used a variety of rodent and human experimental model systems to determine whether exercise training can reduce tumor burden in breast cancer and to identify mechanism associated with any exercise training effects on tumor burden.Results:We show that voluntary wheel running slows tumor development in the mammary specific polyomavirus middle T antigen overexpression(MMTV-PyMT)mouse model of breast cancer but only when mice are not housed alone.We identify the proteoglycan decorin as a contraction-induced secretory factor that systemically increases in patients with breast cancer immediately following exercise.Moreover,high expression of decorin in tumors is associated with improved prognosis in patients,while treatment of breast cancer cells in vitro with decorin reduces cell proliferation.Notwithstanding,when we overexpressed decorin in murine muscle or injected recombinant decorin systemically into mouse models of breast cancer,elevated plasma decorin concentrations did not result in higher tumor decorin levels and tumor burden was not improved.Conclusion:Exercise training is anti-tumorigenic in a mouse model of luminal breast cancer,but the effect is abrogated by social isolation.The proteoglycan decorin is an exercise-induced secretory protein,and tumor decorin levels are positively associated with improved prognosis in patients.The hypothesis that elevated plasma decorin is a mechanism by which exercise training improves breast cancer progression in humans is not,however,supported by our pre-clinical data since elevated circulating decorin did not increase tumor decorin levels in these models. 展开更多
关键词 Breast cancer Exercise training Muscle secretory factors Proteoglycans
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Paracrine and endocrine actions of bone——the functions of secretory proteins from osteoblasts, osteocytes, and osteoclasts 认领 引用 被引量：95
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作者 Yujiao Han Xiuling You +2 位作者 Wenhui Xing Zhong Zhang Weiguo Zou 《Bone Research》 SCIE CAS CSCD 2018年第2期121-131,共11页
The skeleton is a dynamic organ that is constantly remodeled. Proteins secreted from bone cells, namely osteoblasts, osteocytes,and osteoclasts exert regulation on osteoblastogenesis, osteclastogenesis, and angiogenes... The skeleton is a dynamic organ that is constantly remodeled. Proteins secreted from bone cells, namely osteoblasts, osteocytes,and osteoclasts exert regulation on osteoblastogenesis, osteclastogenesis, and angiogenesis in a paracrine manner. Osteoblasts secrete a range of different molecules including RANKL/OPG, M-CSF, SEMA3A, WNT5A, and WNT16 that regulate osteoclastogenesis. Osteoblasts also produce VEGFA that stimulates osteoblastogenesis and angiogenesis. Osteocytes produce sclerostin（SOST） that inhibits osteoblast differentiation and promotes osteoclast differentiation. Osteoclasts secrete factors including BMP6, CTHRC1, EFNB2, S1P, WNT10B, SEMA4D, and CT-1 that act on osteoblasts and osteocytes, and thereby influencea A osteogenesis. Osteoclast precursors produce the angiogenic factor PDGF-BB to promote the formation of Type H vessels, which then stimulate osteoblastogenesis. Besides, the evidences over the past decades show that at least three hormones or ＂osteokines＂from bone cells have endocrine functions. FGF23 is produced by osteoblasts and osteocytes and can regulate phosphate metabolism. Osteocalcin（OCN） secreted by osteoblasts regulates systemic glucose and energy metabolism, reproduction, and cognition. Lipocalin-2（LCN2） is secreted by osteoblasts and can influence energy metabolism by suppressing appetite in the brain.We review the recent progresses in the paracrine and endocrine functions of the secretory proteins of osteoblasts, osteocytes, and osteoclasts, revealing connections of the skeleton with other tissues and providing added insights into the pathogenesis of degenerative diseases affecting multiple organs and the drug discovery process. 展开更多
关键词 Paracrine endocrine actions bone functions secretory proteins osteoblasts osteoclasts osteocytes
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Clinicopathologic and molecular characteristics of 44 patients with pure secretory breast carcinoma 认领 引用 被引量：11
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作者 Lijuan Li Nan Wu +3 位作者 Fangxuan Li Lingmei Li Lijuan Wei Juntian Liu 《Cancer Biology & Medicine》 SCIE CAS CSCD 2019年第1期139-146,共8页
Objective: Secretory breast carcinoma(SBC) is a rare type of breast malignancy, accounting for less than 0.02% of all infiltrating breast malignancies. The pure SBC, a type of SBC without another type of breast malign... Objective: Secretory breast carcinoma(SBC) is a rare type of breast malignancy, accounting for less than 0.02% of all infiltrating breast malignancies. The pure SBC, a type of SBC without another type of breast malignant neoplasm, is particularly rare. This study aimed to investigate the clinicopathologic and molecular features of pure SBC.Methods: The main pathological parameters such as estrogen receptor(ER), progesterone receptor(PR), and human epithelial growth factor receptor 2(C-erbB-2) were detected by immunohistochemistry(IHC), and the clinicopathologic and prognostic difference were compared with invasive ductal carcinoma(IDC). Fluorescent in situ hybridization(FISH) and reverse transcription polymerase chain reaction(RT-PCR) was performed to identify the ETV6-NTRK3 rearrangement of SBC.Results: We found that the positivity rates of ER, PR, C-erbB-2, p53, and S-100 were 47.7%(21/44), 52.3%(23/44), 36.4%(16/44), 27.3%(12/44), and 95.5%(42/44), respectively, which were higher than those reported in previous studies. Special periodic acid-Schiff analysis was performed in 36 patients, and the value of the Ki-67 index ranged from 1% to 50%(mean value:10%). Interestingly, most patients with pure SBC harbored an ETV6-NTRK3 rearrangement with an 88.6%(39/44) expression rate. Compared with IDC, the tumor size of most patients with SBC was larger than 2 cm(P = 0.024). Ultrasound showed benign lesions, and the total misdiagnosis rate was higher(P = 0.020). Although the pathological classification was mostly triple-negative breast cancers(P = 0.036), there was less metastasis(P = 0.029), and the overall prognosis was better than that of the IDC group.Conclusions: Although axillary lymph node metastasis, local recurrence, or distant metastasis may occur, SBC is also considered an indolent neoplasm with a good prognosis. Once diagnosed, surgical treatment should be performed as soon as possible,followed by appropriate adjuvant chemotherapy, irradiation, and endocrine therapies. 展开更多
关键词 Breast cancer pure secretory breast carcinoma clinicopathologic feature therapeutics and prognosis
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Structure and function of epididymal protein cysteine-rich secretory protein-1 认领 引用 被引量：9
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作者 Kenneth P. Roberts Daniel S. Johnston +5 位作者 Michael A. Nolan Joseph L. Wooters Nicole C. Waxmonsky Laura B. Piehl Kathy M. Ensrud-Bowlin David W. Hamilton 《Asian Journal of Andrology》 SCIE CAS 2007年第4期508-514,共7页
Cysteine-rich secretory protein-1 （CRISP-1） is a glycoprotein secreted by the epididymal epithelium. It is a member of a large family of proteins characterized by two conserved domains and a set of 16 conserved cyst... Cysteine-rich secretory protein-1 （CRISP-1） is a glycoprotein secreted by the epididymal epithelium. It is a member of a large family of proteins characterized by two conserved domains and a set of 16 conserved cysteine residues. In mammals, CRISP-1 inhibits sperm-egg fusion and can suppress sperm capacitation. The molecular mechanism of action of the mammalian CRISP proteins remains unknown, but certain non-mammalian CRISP proteins can block ion channels. In the rat, CRISP-1 comprises two forms referred to as Proteins D and E. Recent work in our laboratory demonstrates that the D form of CRISP-1 associates transiently with the sperm surface, whereas the E form binds tightly. When the spermatozoa are washed, the E form of CRISP-1 persists on the sperm surface after all D form has dissociated. Cross-linking studies demonstrate different protein-protein interaction patterns for D and E, although no binding partners for either protein have yet been identified. Mass spectrometric analyses revealed a potential post-translational modification on the E form that is not present on the D form. This is the only discernable difference between Proteins D and E, and presumably is responsible for the difference in behavior of these two forms of rat CRISP- 1. These studies demonstrate that the more abundant D form interacts with spermatozoa transiently, possibly with a specific receptor on the sperm surface, consistent with a capacitation-suppressing function during sperm transit and storage in the epididymis, and also confirm a tightly bound population of the E form that could act in the female reproductive tract. （Asian J Androl 2007 July; 9： 508-514） 展开更多
关键词 cysteine-rich secretory protein-1 epididymis sperm capacitation
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Balloon dilation of Eustachian tube combined with tympanostomy tube insertion and middle ear pressure equalization therapy for recurrent secretory otitis media 认领 引用 被引量：13
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作者 Gendi Yin Jingqian Tan Peng Li 《Journal of Otology》 CAS CSCD 2019年第3期101-105,共5页
Objective: To report outcomes of balloon dilation Eustachian tuboplasty combined with tympanostomy tube insertion and middle ear pressure equalization therapy in treatment of recurrent secretory otitis media. Methods:... Objective: To report outcomes of balloon dilation Eustachian tuboplasty combined with tympanostomy tube insertion and middle ear pressure equalization therapy in treatment of recurrent secretory otitis media. Methods: Fifty one patients with recurrent secretory otitis media (62 ears) underwent balloon dilation of Eustachian tube and tympanic tube insertion under general anesthesia, followed by long term middle ear pressure equalization therapies. The Eustachian tube score (ETS) and Eustachian tube function questionnaire (ETDQ-7) were used for pre- and postoperative (up to 12 months) evaluation of Eustachian tube functions. Results: The mean ETS score was 2.34 ± 0.97 preoperatively, and 6.17 ± 1.54, 7.23 ± 1.62, 8.24 ± 1.97, and 7.63 ± 1.86 at 1, 3, 6 and 12 months postoperatively, respectively (P < 0.05). The ETDQ-7 score was 4.82 ± 1.07 preoperatively, and 2.20 ± 0.54, 2.32 ± 0.68, 2.53 ± 0.79, and 2.67 ± 0.76 at 1, 3, 6 and 12 months postoperatively, respectively (P < 0.05). Conclusion: Balloon dilation of Eustachian tube combined with tympanostomy and catheterization resulted in significant improvement of subjective symptoms and objective evaluation of Eustachian tube functions in most patients with recurrent secretory otitis media, as indicated by the ETS and ETDQ-7 scores, demonstrating high levels of efficacy and patient satisfaction. 展开更多
关键词 Balloon dilation eustachian tuboplasty Positive and negative auricular pressure therapy Chronic recurrent secretory otitis media Eustachian tube function score Eustachian tube function questionnaire-7
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MicroRNA-27a-mediated repression of cysteine-rich secretory protein 2 translation in asthenoteratozoospermic patients 认领 引用 被引量：7
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作者 Jun-Hao Zhou Qi-Zhao Zhou +9 位作者 Jian-Kun Yang Xiao-Ming Lyu Jun Bian Wen-Bin Guo Zi-Jian Chen Ming Xia Hui Xia Tao Qi Xin Li Cun-Dong Liu 《Asian Journal of Andrology》 SCIE CAS CSCD 2017年第5期591-595,共5页
Cysteine-rich secretory protein 2 （CRISP2） is an important protein in spermatozoa that plays roles in modulating sperm flagellar motility, the acrosome reaction, and gamete fusion. Spermatozoa lacking CRISP2 exhibit... Cysteine-rich secretory protein 2 （CRISP2） is an important protein in spermatozoa that plays roles in modulating sperm flagellar motility, the acrosome reaction, and gamete fusion. Spermatozoa lacking CRISP2 exhibit low sperm motility and abnormal morphology. However, the molecular mechanisms underlying the reduction of CRISP2 in asthenoteratozoospermia （ATZ） remain unknown. In this study, low expression of CRISP2 protein rather than its mRNA was observed in the ejaculated spermatozoa from ATZ patients as compared with normozoospermic males. Subsequently, bioinformatic prediction, luciferase reporter assays, and microRNA-27a （miR-27a） transfection experiments revealed that miR-27a specifically targets CRISP2 by binding to its 3＇ untranslated region （3＇-UTR）, suppressing CRISP2 expression posttranscriptionally. Further evidence was provided by the clinical observation of high miR-27a expression in ejaculated spermatozoa from ATZ patients and a negative correlation between miR-27a expression and CRISP2 protein expression. Finally, a retrospective follow-up study supported that both high miR-27a expression and low CRISP2 protein expression were associated with low progressive sperm motility, abnormal morphology, and infertility. This study demonstrates a novel mechanism responsible for reduced CRISP2 expression in ATZ, which may offer a potential therapeutic target for treating male infertility, or for male contraception. 展开更多
关键词 asthenoteratozoospermia cysteine-rich secretory protein 2 male infertility microRNA-27a
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Gene expression profiling defined pathways correlated with fibroblast cell proliferation induced by Opisthorchis viverrini excretory /secretory product 认领 引用 被引量：5
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作者 Chanitra Thuwajit Peti Thuwajit +4 位作者 Kazuhiko Uchida Daoyot Daorueang Sasithorn Kaewkes Sopit Wongkham Masanao Miwa 《World Journal of Gastroenterology》 SCIE CAS 2006年第22期3585-3592,共8页
AIM： To investigate the mechanism of fibroblast cell proliferation stimulated by the Opisthorchis viverrini excretory/secretory （ES） product. METHODS： NIH-3T3, mouse fibroblast cells were treated with O. viverrini... AIM： To investigate the mechanism of fibroblast cell proliferation stimulated by the Opisthorchis viverrini excretory/secretory （ES） product. METHODS： NIH-3T3, mouse fibroblast cells were treated with O. viverrini ES product by non-contact co-cultured with the adult parasites. Total RNA from NIH-3T3 treated and untreated with O. viverrini was extracted, reverse transcribed and hybridized with the mouse 15K complementary DNA （cDNA） array. The result was analyzed by ArrayVision version 5 and GeneSpring version 5 softwares. After normalization, the ratios of gene expression of parasite treated to untreated NIH-3T3 cells of 2-and more-fold upregulated was defined as the differentially expressed genes. The expression levels of the signal transduction genes were validated by semiquantitative SYBR-based real-time RT-PCR. RESULTS： Among a total of 15 000 genes/ESTs, 239 genes with established cell proliferation-related function were 2 fold-and more-up-regulated by O. viverrini ES product compared to those in cells without exposure to the parasitic product. These genes were classified into groups including energy and metabolism, signal transduction, protein synthesis and translation, matrix and structural protein, transcription control, cell cycle and DNA replication. Moreover, the expressions of serinethreonine kinase receptor, receptor tyrosine kinase and collagen production-related genes were up-regulated by O.viverrini ES product, The expression level of signal transduction genes, pkC, pdgfra, jak 1, eps 8, tgfβ 1/4,strap and h ras measured by real-time RT-PCR confirmed their expression levels to those obtained from cDNA array. However, only the up-regulated expression of pkC,eps 8 and tgfβ3 1/4 which are the downstream signaling molecules of either epidermal growth factor （EGF） or transforming growth factor-β （TGF-β） showed statistical significance （P 〈 0.05）.CONCLUSION： O. viverrini ES product stimulates the significant changes of gene expression in several functional categories and these mainly include transcripts related to cell proliferation. The TGF-β and EGF signal transduction pathways are indicated as the possible pathways of O. viverrini-driven cell proliferation. 展开更多
关键词 Gene expression profile Opisthorchis viverrini Excretory/secretory product cDNA array Fibroblast Cell proliferation Signal transduction Cholangiocarcinogenesis
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SsdchA is a novel secretory cellobiohydrolase driving pathogenicity in Sclerotinia sclerotiorum 认领 引用 被引量：4
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作者 Yangui Chen Yijuan Ding +8 位作者 Siqi Zhao Nan Yang Zhaohui Wu Ping Zhang Hongmei Liao Mengquan Dong Yang Yu Huafang Wan Wei Qian 《The Crop Journal》 SCIE CSCD 2024年第2期493-502,共10页
The necrotrophic fungus, Sclerotinia sclerotiorum, employs an array of cell wall-degrading enzymes(CWDEs), including cellulase, to dismantle host cell walls. However, the molecular mechanisms through which S. scleroti... The necrotrophic fungus, Sclerotinia sclerotiorum, employs an array of cell wall-degrading enzymes(CWDEs), including cellulase, to dismantle host cell walls. However, the molecular mechanisms through which S. sclerotiorum degrades cellulose remain elusive. Here, we unveil a novel secretory cellobiohydrolase, SsdchA, characterized by a signal peptide and a Glyco_hydro_7(GH7) domain. SsdchA exhibits a robust expression of during early infection stages. Interestingly, colony morphology and growth rates remain unaffected across the wild-type, SsdchA deletion strains and SsdchA overexpression strains on potato dextrose agar(PDA) medium. Nevertheless, the pathogenicity and cellobiohydrolase activity decreased in the SsdchA deletion strains, but enhanced in the SsdchA overexpression strains. Moreover,the heterologous expression of SsdchA in Arabidopsis thaliana leads to reduced cellulose content and heightened susceptibility to S. sclerotiorum. Collectively, our data underscore the pivotal role of the novel cellobiohydrolase SsdchA in the pathogenicity of S. sclerotiorum. 展开更多
关键词 Cellobiohydrolase Cellulose Pathogenicity Sclerotinia sclerotiorum Secretory protein SsdchA
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Animal secretory endolysosome channel discovery 认领 引用 被引量：6
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作者 Yun Zhang Qi-Quan Wang +1 位作者 Zhong Zhao Cheng-Jie Deng 《Zoological Research》 SCIE CSCD 2021年第2期141-152,共12页
Secretory pore-forming proteins(PFPs) have been identified in organisms from all kingdoms of life. Our studies with the toad species Bombina maxima found an interaction network among aerolysin family PFPs(af-PFPs) and... Secretory pore-forming proteins(PFPs) have been identified in organisms from all kingdoms of life. Our studies with the toad species Bombina maxima found an interaction network among aerolysin family PFPs(af-PFPs) and trefoil factors(TFFs). As a toad af-PFP, Bm ALP1 can be reversibly regulated between active and inactive forms, with its paralog Bm ALP3 acting as a negative regulator. Bm ALP1 interacts with Bm TFF3 to form a cellular active complex called βγ-CAT. This PFP complex is characterized by acting on endocytic pathways and forming pores on endolysosomes, including stimulating cell macropinocytosis. In addition, cell exocytosis can be induced and/or modulated in the presence of βγ-CAT. Depending on cell contexts and surroundings, these effects can facilitate the toad in material uptake and vesicular transport, while maintaining mucosal barrier function as well as immune defense. Based on experimental evidence,we hereby propose a secretory endolysosome channel(SELC) pathway conducted by a secreted PFP in cell endocytic and exocytic systems, with βγ-CAT being the first example of a SELC protein. With essential roles in cell interactions and environmental adaptations, the proposed SELC protein pathway should be conserved in other living organisms. 展开更多
关键词 Pore-forming protein Secretory endolysosome channel(SELC) Endocytosis Exocytosis Vesicular transport
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Expression of serum human epididymal secretory protein E4 at low grade and high grade serous carcinomas 认领 引用 被引量：4
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作者 Ya-Fei Zhu Lin-Sheng He +1 位作者 Zhen-Dong Zhang Qing-Shui Huang 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2012年第12期925-930,共6页
Objective:To investigate the value of serum human epididymis protein 4(HE4) in differential diagnosis of patients with low-grade serous(LGSC) and high-grade serous carcinoma(HGSC) serous ovarian cancer.Methods:LGSC an... Objective:To investigate the value of serum human epididymis protein 4(HE4) in differential diagnosis of patients with low-grade serous(LGSC) and high-grade serous carcinoma(HGSC) serous ovarian cancer.Methods:LGSC and HGSC serous ovarian cancer were diagnosed by the two-tier grade system,serum levels of HE4 and carbohydrate antigen 12S(CA125) were measured by ELBA and radioisotope method,respectively in 60 serous ovarian cancer patients. HE4 and TPS3 protein in cancer tissue were measured by immunohistochemical method. Results:The difference in density of HE4 and TP53 protein was significant between LGSC and HGSC tissue,while serum CA12S did not show significant difference between different serum samples.There was significant difference in serum HE4 levels between LGSC and HGSC and the result was different within FIGO(Ⅰ+Ⅱ) stage,suggesting HE4 was not a reliable biomarker for the discrimination between LGSC and HCSC.HE4 had potential as a biomarker for the discrimination between LGSC and HGSC but the role in early diagnosis was limited.Conclusions:HE4 may be a reliable marker for differential diagnosis of LGSC and HGSC.But its role in early diagnosis of LGSC and HGSC need to be confirmed from the perspective of two-tier grade system. 展开更多
关键词 Human epididymal secretory protein E4 Ovarian neoplasms Heterogeneity Early diagnosis Dualistic model Two-tier grade system
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Construction and Secretory Expression of β-Galactosidase Gene from Lactobacillus Bulgaricus in Lactococcus Lactis 认领 引用 被引量：4
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作者 ZHANG Wen WANG Chuan +4 位作者 HUANG Cheng Yu YU Qian LIU Heng Chuan ZHANG Chao Wu PEI Xiao Fang 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2012年第2期203-209,共7页
Objective This study is to examine the secretion effects of β-galactosidase in Lactococcus lactis.Methods The usp45 and β-galactosidase genes were cloned and inserted into plasmid pMG36e to obtain the recombinant pl... Objective This study is to examine the secretion effects of β-galactosidase in Lactococcus lactis.Methods The usp45 and β-galactosidase genes were cloned and inserted into plasmid pMG36e to obtain the recombinant plasmid pMG36e-usp-lacZ.This recombinant plasmid was transformed into both Escherichia coli DH5α and L.lactis MG1363.The enzyme activity,gene sequencing,SDS-PAGE and hereditary stability were assessed and studied.Results The lacZ gene inserted into plasmids pMG36e-usp-lacZ was 99.37% similar to the GenBank sequence,and SDS-PAGE revealed an evident idio-strap at 116 KDa between L.lactis MG1363/pMG36eusp-lacZ in both supernatant and cell samples.β-Galactosidase activity measured 0.225 U/mL in L.lactis pMG36e-usp-lacZ transformants,and its secretion rate was 10%.The plasmid pMG36e-usp-lacZ appeared more stable in MG1363.Conclusion The authors concluded that these new recombinant bacteria well expressed and secreted β-galactosidase,indicating that the β-galactosidase expression system was successfully constructed,and this might provide a new solution for management of lactose intolerance specifically and promote the use of gene-modified organisms as part of the food-grade plasmid in general. 展开更多
关键词 Gene constructs Gene expression Secretory expression β-Galactosidase Lactococcus lactis
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High-efficiency secretory expression and characterization of the recombinant type Ⅲ human-like collagen in Pichia pastoris 认领 引用 被引量：4
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作者 Zhi-Xiang Xiang Jin-Song Gong +6 位作者 Jin-Hao Shi Chun-Fang Liu Heng Li Chang Su Min Jiang Zheng-Hong Xu Jin-Song Shi 《Bioresources and Bioprocessing》 SCIE 2022年第1期189-201,共13页
Collagen,the highest content protein in the body,has irreplaceable biological functions,and it is widespread concerned in food,beauty,and medicine with great market demand.The gene encoding the recombinant type Ⅲ hum... Collagen,the highest content protein in the body,has irreplaceable biological functions,and it is widespread concerned in food,beauty,and medicine with great market demand.The gene encoding the recombinant type Ⅲ human-like collagen α1 chain fragment was integrated into P.pastorisgenome after partial amino acids were substituted.Combined with promoter engineering and high-density fermentation technology,soluble secretory expression with the highest yield of 1.05 g L⁻¹ was achieved using two-stage feeding method,and the purity could reach 96%after affinity purification.The determination of N/C-terminal protein sequence were consistent with the theoretical expectation and showed the characteristics of Gly-X-Y repeated short peptide sequence.In amino acid analysis,glycine shared 27.02%and proline 23.92%,which were in accordance with the characteristics of collagen.Ultraviolet spectrum combined with Fourier transform infrared spectroscopy as well as mass spectrometry demonstrated that the target product conformed to the characteristics of collagen spectrums and existed as homologous dimer and trimer in the broth.This work provided a sustainable and economically viable source of the recombinant type Ⅲ human-like collagen. 展开更多
关键词 Human-like collagen Secretory expression Fermentation Protein purification Characterization
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Secretory Transactivating Transcription-apoptin fusion protein induces apoptosis in hepatocellular carcinoma HepG2 cells 认领 引用 被引量：2
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作者 Su-Xia Han Jin-Lu Ma +3 位作者 Yi Lv Chen Huang Hai-Hua Liang Kang-Min Duan 《World Journal of Gastroenterology》 SCIE CAS 2008年第23期3642-3649,共8页
AIM:To determine whether SP-TAT-apoptin induces apoptosis and also maintains its tumor cell specificity. METHODS:In this study,we designed a secretory protein by adding a secretory signal peptide(SP) to the N terminus... AIM:To determine whether SP-TAT-apoptin induces apoptosis and also maintains its tumor cell specificity. METHODS:In this study,we designed a secretory protein by adding a secretory signal peptide(SP) to the N terminus of Transactivating Transcription(TAT)-apoptin(SP-TAT-apoptin),to test the hypothesis that it gains an additive bystander effect as an anti-cancer therapy. We used an artificial human secretory SP whose amino acid sequence and corresponding cDNA sequence were generated by the SP hidden Markov model. RESULTS:In human liver carcinoma HepG2 cells,SP-TAT-apoptin expression showed a diffuse pattern in the early phase after transfection. After 48 h,however,it translocated into the nuclear compartment and caused massive apoptotic cell death,as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay and annexin-V binding assay. SP-TAT-apoptin did not,however,cause any cell death in non-malignant human umbilical vein endothelial cells(HUVECs). Most importantly,the conditioned medium from Chinese hamster ovary(CHO) cells transfected with SP-TAT-apoptin also induced significant cell deathin HepG2 cells,but not in HUVECs. CONCLUSION:The data demonstrated that SP-TAT-apoptin induces apoptosis only in malignant cells,and its secretory property might greatly increase its potency once it is delivered in vivo for cancer therapy. 展开更多
关键词 Apoptin Apoptosis Hepatoma Human Immunodeficiency Virus-Transactivating Transcription protein Secretory
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