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Development of nontarget method based on GC-QTOF-HRMS for analyzing organic pollutants in human serum 认领 引用 被引量：1
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作者 Congcong Yue Chang He +5 位作者 Hailing Li Zhiquan Yuan Guiying Li Shengtao Ma Xin Zhang Taicheng An 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2026年第7期379-386,共8页
Traditional targeted analyses often overlook unknown or emerging contaminants,highlighting the significance of nontarget and suspect screening approaches.A novel and high-sensitivity methodology for nontarget analysis... Traditional targeted analyses often overlook unknown or emerging contaminants,highlighting the significance of nontarget and suspect screening approaches.A novel and high-sensitivity methodology for nontarget analysis of organic pollutants in human serum was newly-developed based on gas chromatography coupled with quadrupole time-of-flight high-resolution mass spectrometry.The extraction protocol employing an acetonitrile-ethyl acetate(9:1,V:V)mixture significantly improved the extraction efficiency while minimizing matrix effect.A hybridized analytical strategy integrating nontarget and suspect screening was developed to achieve comprehensive identification and classification of pollutants,employing the National Institute of Standards and Technology(NIST)20 library and Agilent Technologies Personal Compound Database and Library(PCDL).This approach successfully characterized 273 organic contaminants spanning 12 categories,including polycyclic aromatic hydrocarbons(PAHs)and their derivatives,esters,and phenolic compounds in human serum,with a significant increase in detection specificity compared to conventional workflows.The methodology used serum samples of the workers from coking industry,revealing widespread contamination dominated by PAHs and PAH derivatives.Among the target analytes,three were identified solely by NIST and six solely by PCDL,indicating the complementary benefits of combining these different databases.Notably,this work reported the first confirmed detection of 2-naphthalenamine in human serum.This optimized approach demonstrates enhanced sensitivity and reliability in serum analysis,advancing biomonitoring capabilities and providing a deep understanding of human exposure to environmental pollutants. 展开更多
关键词 Nontarget analysis Biomonitoring method Human serum sample Organic pollutants GC-QTOF-HRMS
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Causal relationships of inflammatory cytokines and serum metabolites with colorectal carcinoma: A Mendelian randomization study 认领 引用 被引量：1
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作者 FENG Sisi XIAO Xiaomin +2 位作者 ZHOU Manli HUANG Zixin ZHONG Baiyun 《中南大学学报（医学版）》 CAS CSCD 北大核心 2026年第1期118-128,共11页
Objective:The incidence and mortality of colorectal carcinoma(CRC)continue to rise globally,highlighting the need to identify modifiable risk factors for early detection and prevention.Previous studies have demonstrat... Objective:The incidence and mortality of colorectal carcinoma(CRC)continue to rise globally,highlighting the need to identify modifiable risk factors for early detection and prevention.Previous studies have demonstrated significant associations between CRC risk and various serum metabolites as well as inflammatory cytokines;however,due to limitations in study design and potential confounding factors,the causal relationships remain unclear.This study aims to investigate the causal relationships between inflammatory cytokines,serum metabolites,and CRC risk,providing a theoretical basis for the development of novel early diagnostic biomarkers and therapeutic targets.Methods:A two-sample Mendelian randomization(MR)design was applied using summary statistics from genome-wide association studies(GWAS).Instrumental variables(IVs)were derived from:1)metabolomics GWAS data of 1400 serum metabolites(n=8299);2)cytokine GWAS data of 91 inflammatory factors(n=14824);and 3)CRC risk data from the FinnGen consortium(6847 cases and 314193 controls).The primary analysis was conducted using the inverse-variance weighted(IVW)method,with sensitivity analyses performed using MR Egger regression and the weighted median method.Effect estimates including odds ratios(OR),95%confidence intervals(CI),and false discovery rates(FDR)were calculated.Results:MR analysis indicated that higher levels of axin-1(AXIN1)(OR=0.84195%CI 0.714 to 0.991)and Fms-related tyrosine kinase 3 ligand(Flt3L)(OR=0.916,95%CI 0.844 to 0.994)were associated with a reduced risk of CRC.In contrast,higher levels of Delta/Notchlike epidermal growth factor-related receptor(DNER)(OR=1.119,95%CI 1.009 to 1.241)and vascular endothelial growth factor A(VEGF-A)(OR=1.078,95%CI 1.011 to 1.150)were associated with an increased risk of CRC(all P<0.05).Metabolomics association analysis further identified 144 serum metabolites significantly correlated with these four key inflammatory cytokines(FDR<0.05),suggesting that they may regulate CRC risk through inflammatory pathways.Conclusion:Specific inflammatory cytokines and serum metabolites have causal relationships with the risk of CRC.These findings provide insights for further exploration of potential risk factors and the development of effective prevention strategies for CRC. 展开更多
关键词 inflammatory cytokines serum metabolites colorectal carcinoma Mendelian randomization genome-wide association study metabolomics analysis
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Blood serum from individuals with Alzheimer’s disease alters microglial phagocytosis in vitro 认领 引用 被引量：1
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作者 Barbara Altendorfer Rodolphe Poupardin +21 位作者 Sophie Lefèvre-Arbogast Claudine Manach Dorrain Y.Low Mireia Urpi-Sarda Cristina Andres-Lacueva Raúl González-Domínguez Thomas K.Felder Julia Tevini Marco Zattoni Andreas Koller Reinhold Schmidt Paul J.Lucassen Silvie R.Ruigrok Chiara de Lucia Andrea Du Preez Catherine Helmer Jeanne Neuffer Cécile Proust-Lima Aniko Korosi Cécilia Samieri Sandrine Thuret Ludwig Aigner 《Neural Regeneration Research》 SCIE CAS CSCD 2026年第6期2433-2439,共7页
In Alzheimer’s disease,microglial phagocytosis is engaged in the pathogenesis as it clears abnormal protein accumulations,debris,and apoptotic cells in the early stages of Alzheimer’s disease,but fuels neuroinflamma... In Alzheimer’s disease,microglial phagocytosis is engaged in the pathogenesis as it clears abnormal protein accumulations,debris,and apoptotic cells in the early stages of Alzheimer’s disease,but fuels neuroinflammation and accelerates disease progression in later stages.In vivo parabiosis experiments in aged animals have demonstrated that blood-born factors modulate synaptic plasticity,neurogenesis,and microglial responses.We hypothesize that peripheral factors can modulate microglial function and thereby possibly influence Alzheimer’s disease pathology.The objective of this study is to investigate the effects of Alzheimer’s disease serum on microglial phagocytosis.Here,we use an immortalized human microglial cell line in an in vitro parabiosis assay to investigate the impact of the serum from individuals diagnosed with Alzheimer’s disease(n=30)and age-matched controls(n=30)(PRODEM study)on microglial phagocytosis.Exposure to Alzheimer’s disease serum increased microglial phagocytic uptake of pH-sensitive fluorescent particles and downregulated expression of the lysosomal master regulator transcription factor EB(TFEB)and of ATPase H+transporting lysosomal V1 subunit B2(ATP6V1B2),a component of the vacuolar ATPase.To identify serum components that may relate to changes in phagocytosis,serum samples of the Three-City Study(3C Study)were used.In the 3C Study,blood samples were collected up to 12 years before the onset of cognitive decline or dementia and their serum metabolome is well-defined.Microglia exposed to the serum of future Alzheimer’s disease patients from the 3C Study displayed an increased phagocytic uptake compared with the serum of matched controls,depending on the presence of the apolipoprotein Eε4 allele in the Alzheimer’s disease patients.Furthermore,microglial phagocytosis correlated inversely with serum levels of the omega-3 fatty acid eicosapentaenoic acid.We confirmed this inverse correlation between eicosapentaenoic acid and phagocytosis in the serum samples of the PRODEM cohort.In addition,in vitro testing of eicosapentaenoic acid on microglial phagocytosis showed a concentration-dependent decrease in phagocytic uptake.In conclusion,following incubation with Alzheimer’s disease blood serum,we observed increased microglial phagocytic uptake and the downregulation of TFEB and ATP6V1B2,possibly indicating lysosomal dysfunction.Furthermore,microglial phagocytosis was inversely correlated with serum eicosapentaenoic acid levels,suggesting an important role for dietary eicosapentaenoic acid in microglial function. 展开更多
关键词 Alzheimer’s disease blood serum eicosapentaenoic acid in vitro parabiosis metabolome microglia omega-3 fatty acids phagocytosis
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Elevated serum osmolarity is associated with 28-day all-cause mortality in patients with cardiac arrest 认领 引用
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作者 Ping Gong Hong Zhao +4 位作者 Peijuan Li Ling Wang Jin Wang Rui Yang Zhangping Sun 《World Journal of Emergency Medicine》 SCIE CAS CSCD 2026年第1期50-56,共7页
BACKGROUND:Serum osmolality is a prognostic indicator in critically ill patients.This study aimed to evaluate the association between high osmolality and 28-day mortality in patients with cardiac arrest(CA)admitted to... BACKGROUND:Serum osmolality is a prognostic indicator in critically ill patients.This study aimed to evaluate the association between high osmolality and 28-day mortality in patients with cardiac arrest(CA)admitted to the intensive care unit(ICU).METHODS:Baseline data of adult patients with CA who were admitted to the ICU from 2008 to 2019 were collected from the Medical Information Mart for Intensive Care(MIMIC)-IV.Patients were divided into survivor and non-survivor groups according to the 28-day prognosis.Serum concentrations of sodium,potassium,glucose,and urea nitrogen on the fi rst day of ICU admission were used to determine serum osmolarity.The primary endpoint of this study was 28-day all-cause mortality.Propensity score matching(PSM)analysis was performed to reduce bias between the survivor and nonsurvivor groups.RESULTS:Among the 798 included CA patients,the high osmolarity on the first day of ICU admission remained significantly associated with increased 28-day mortality(62.0%vs.35.5%,P<0.001)and reduced cumulative survival(log-rank P<0.05)after PSM.Cox regression identifi ed the high osmolarity on the fi rst day of ICU admission as an independent predictor.High serum osmolarity on the fi rst day of ICU admission eff ectively predicted 1-,3-,7-,and 28-day all-cause mortality,with the strongest predictive performance for 1-day mortality both before and after PSM(all P<0.05).CONCLUSION:In this study,elevated serum osmolarity on the first day of ICU admission was independently associated with increased 28-day mortality in CA patients and could serve as a prognostic marker. 展开更多
关键词 Cardiac arrest Serum osmolarity High osmolarity Mortality
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Tracing the key influencing factors and predicting the concentration trend of perfluoroalkyl substances(PFASs)in human serum and plasma 认领 引用
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作者 Baolin Liu Xinyu Ma +2 位作者 Junjie Li Sixu Liu Yong Yu 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2026年第4期355-365,共11页
This review completed 58,647 data acquisition over 30 years from the published literatures to investigate the distribution,influencing factors and future levels of 15 perfluoroalkyl substances(PFASs)in human serum and... This review completed 58,647 data acquisition over 30 years from the published literatures to investigate the distribution,influencing factors and future levels of 15 perfluoroalkyl substances(PFASs)in human serum and plasma.Perfluorooctane sulfonate(PFOS)is the predominant PFAS with a mean of 11.8 ng/mL in serum and 9.32 ng/mL in plasma,accounting more than 50%of the total of 15 PFASs(Σ15PFASs)in most countries.Industrial emission,dietary ingestion and exposure of clothing and paper for packaging food are the primary sources of PFASs in human blood,contributing 41.6%,21.3%and 17.5%ofΣ15PFASs,respectively.HighΣ15PFASs are found in serum and plasma from Sweden,Australia,United States and China.A significant declining trend is observed in serum and plasma concentrations of PFOS after 2009.Moreover,gender,pregnancy and exposure significantly influence the blood concentrations of some PFAS congeners.The levels of PFOS,perfluorooctanoic acid,perfluorohexane sulfonic acid in both serum and plasma are predicted to decrease significantly in the next two decades,while those of perfluorodecanoic acid in serum may increase by 69.3%.This review contributes a valuable perspective for understanding the occurrence,sources,influencing factors and future levels of PFASs in human blood. 展开更多
关键词 Perfluoroalkyl substances(PFASs) Human serum and plasma Source Influencing factors Future concentration
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Mechanistic investigation of Liujing Toutong tablet in the treatment of cerebral ischemia–reperfusion injury in rats by the integration of serum pharmacochemistry and metabolomics 认领 引用
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作者 Zihan Yu Ke Pei +8 位作者 Xiulin Wang Wenling Li Hongchang Li Wangyang Xie Xianglong Meng Tingting Zhao Yan Ning Haixin Liu Wenbin He 《Animal Models and Experimental Medicine》 CAS CSCD 2026年第6期1105-1125,共21页
Background:Liujing Toutong tablet(LTT)is a traditional Chinese patent medicine.Previous studies have demonstrated that LTT exerts a protective effect in permanent cerebral ischemia and cerebral ischemia–reperfusion i... Background:Liujing Toutong tablet(LTT)is a traditional Chinese patent medicine.Previous studies have demonstrated that LTT exerts a protective effect in permanent cerebral ischemia and cerebral ischemia–reperfusion injury(CIRI).However,the active compounds and underlying mechanisms remain unclear.This investigation aimed to integrate the analyses of serum pharmacochemistry,network pharmacology,and metabolomics to elucidate the therapeutic effects of LTT on CIRI.Methods:The therapeutic effects of LTT were evaluated in the CIRI rat model,which was induced using the thread embolism method.The prototype active compounds of LTT absorbed into the serum(LPCs)were identified using ultra-performance liquid chromatography-quadrupole/orbitrap high-resolution mass spectrometry(UPLC-QExactive-Orbitrap/MS).Potential targets of LPCs were determined using network pharmacological analysis.A combination of metabolomics and network pharmacology was employed to identify upstream key targets and downstream endogenous metabolites.The predicted mechanisms were then verified by molecular docking.Finally,key targets and signaling pathways were examined using enzyme-linked immunosorbent assay(ELISA)and Western blot.Results:A total of 27 LPCs were identified as the active ingredients.Furthermore,glycerophospholipid and arachidonic acid(AA)metabolism were also identified,with PTGS2,ALOX5,and AchE as the upstream key targets,and phosphorylcholine,AA,phosphatidate(PA),hosphatidylcholines(PCs),and lysophosphatidylcholines(LysoPCs)as the core endogenous metabolites.Additionally,LPCs binding with these key targets might reduce the inflammatory response via nuclear factor Kappa-B(NF-κB)signaling pathway.Conclusion:LTT may effectively alleviate symptoms of CIRI by acting on PTGS2,ALOX5,and AchE,improving the glycerophospholipid and AA metabolism and reducing the inflammatory response by inhibiting NF-κB signaling pathway-related proteins. 展开更多
关键词 cerebral ischemia-reperfusion injury Liujing Toutong tablet metabolomics network pharmacology serum pharmacochemistry
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Organophosphate esters and their metabolites in paired maternal serum and human milk:Occurrence,transfer efficiency,and metabolic conversion 认领 引用
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作者 Shunying Yao Lei Zhang +3 位作者 Bing Lyu Yaoyu Tang Jingguang Li Zhixiong Shi 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2026年第4期42-50,共9页
Organophosphate esters(OPEs)and have been frequently detected in human matrices,while the efficiency of their transfer from serum to milk and related influencing factors are unknown.The metabolic transformation of OPE... Organophosphate esters(OPEs)and have been frequently detected in human matrices,while the efficiency of their transfer from serum to milk and related influencing factors are unknown.The metabolic transformation of OPEs to their metabolites(mOPEs)is also less studied.In this study,OPEs and mOPEs were analyzed in paired maternal serum-human milk samples,and they were found to be with high detecting frequencies.Transfer efficiency(TEHM/MS)was calculated to evaluate the ability of OPEs and mOPEs in crossing the blood-milk barrier(BMB).The results indicated large differences in the crossing ability among OPEs/mOPEs,and both passive diffusion and active transport were involved in BMB penetration.Besides molecular weight,the lipid solubility of OPEs and mOPEs plays a key role in their transfer from maternal serum to milk,as TEsHM/MSof both OPEs and mOPEs had significant positive correlations with their n-octane-water partition coefficient(log10Kow).The positive correlation between mOPEs and its parent OPEs in serum indicated that mOPEs were mainly from the metabolism of OPEs.Moreover,the high relative abundance(RA)of some mOPEs proved that their parent compounds were inclined to metabolic conversion,which suggested that the monitoring and risk assessment for mOPEs should not neglected. 展开更多
关键词 Organophosphate esters(OPEs) Blood-milk barrier(BMB) Serum Human milk Maternal transfer
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Serum Endogenous Opioid Levels are Associated with Self-Injury Severity in Adolescents with Non-Suicidal Self-Injury and Comorbid Depression 认领 引用
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作者 Jin-Jun Ding Xiao-Li Liu +11 位作者 Ang Li Yi-Yao Liang Yu-Bo Wang Xiao-Na Zhu Jie Li Jie Weng Lan Yan Wen-Wen Duan Zi-Chen Zhang Ti-Fei Yuan Xiao-Yun Guo Dong-Sheng Zhou 《Neuroscience Bulletin》 SCIE CAS CSCD 2026年第5期1177-1182,共6页
Dear Editor,Non-suicidal self-injury(NSSI)refers to the intentional,self-inflicted damage of body tissues in the absence of suicidal intent and represents a highly prevalent and clinically significant behavioral issue... Dear Editor,Non-suicidal self-injury(NSSI)refers to the intentional,self-inflicted damage of body tissues in the absence of suicidal intent and represents a highly prevalent and clinically significant behavioral issue among adolescents[1]. 展开更多
关键词 adolescents non suicidal self injury self injury severity comorbid depression Serum endogenous opioid levels behavioral issue
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Letter to the Editor:How serum uric acid-to-high-density lipoprotein cholesterol ratio predicts cardiovascular risk in metabolic dysfunction-associated steatotic liver disease:Addressing challenges 认领 引用
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作者 Jing-Juan Xu Jing Ma +2 位作者 Shang Wei Dan Hu Yan-Qi Dang 《World Journal of Gastroenterology》 SCIE CAS 2026年第26期155-158,共4页
Chronic diseases frequently interact,forming complex comorbidity networks.Recent research published in the World Journal of Gastroenterology by Wang et al has identified that the ratio of serum uric acid to high-densi... Chronic diseases frequently interact,forming complex comorbidity networks.Recent research published in the World Journal of Gastroenterology by Wang et al has identified that the ratio of serum uric acid to high-density lipoprotein cholesterol(UHR)independently predicts the 10-year risk of cardiovascular disease(CVD),particularly in younger individuals,males,and those with central obesity.Individuals with metabolic dysfunction-associated steatotic liver disease represent at high-risk cohort for CVD.In these patients,the risk associated with cardiovascular events significantly surpasses that of liver disease,rendering CVD the primary cause of disability and mortality.The UHR is an emerging composite biomarker that effectively synthesizes indices of inflammation and metabolism,facilitating an assessment of a person’s metabolic and inflammatory status.This biomarker exhibits considerable clinical potential. 展开更多
关键词 Metabolic dysfunction-associated steatotic liver disease Cardiovascular risk Serum uric acid-to-high-density lipoprotein cholesterol ratio Emerging composite biomarker Asian patients
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Serum folate and brain-derived neurotrophic factor in pediatric autism spectrum disorder and their predictive role in illness severity 认领 引用
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作者 Li-Bin Chen Xiao-Qin Xu Yan-Hui Chen 《World Journal of Psychiatry》 SCIE 2026年第3期89-98,共10页
BACKGROUND Autism spectrum disorder(ASD)involves social and neurological impairment,and affected individuals have an elevated risk of bullying.AIM To clarify serum folate(SF)and brain-derived neurotrophic factor(BDNF)... BACKGROUND Autism spectrum disorder(ASD)involves social and neurological impairment,and affected individuals have an elevated risk of bullying.AIM To clarify serum folate(SF)and brain-derived neurotrophic factor(BDNF)expression in ASD-affected children and evaluate their prediction value for illness severity.METHODS From February 2023 to February 2025,53 ASD-affected children and 50 healthy controls visiting Fuzhou University Affiliated Provincial Hospital were enrolled as the research and control groups,respectively.SF and BDNF levels were measured in all children.The Childhood Autism Rating Scale(CARS)was used to assess ASD symptom severity.In ASD cases,SF and BDNF expression differences were compared across illness-severity subgroups and before versus after treatment.Pearson r was used to assess correlations between SF/BDNF and CARS in the research group.Receiver operating characteristic(ROC)curves were used to assess their predictive value for ASD severity.Univariate and multivariate binary Logistic models were used to identify ASD progression determinants.RESULTS ASD children showed significantly lower SF and higher BDNF higher than controls.Severe cases had lower SF and higher BDNF than mild-to-moderate cases.SF correlated inversely with the CARS score,whereas BDNF correlated positively.For predicting ASD severity,the area under the ROC curve(AUC)of SF and BDNF was 0.700-0.750,and their combined use increased the AUC to 0.823.Both markers were confirmed to be independent determinants of ASD aggravation.CONCLUSION SF is down-regulated and BDNF is up-regulated in ASD-affected children,SF correlates negatively with ASD severity and BDNF correlates positively.Low SF and high BDNF are risk factors for ASD deterioration in children. 展开更多
关键词 Serum folate Brain-derived neurotrophic factor Autism spectrum disorder Children Illness severity Receiver operating characteristic curve
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Association between serum 25(OH)D levels and cancer in adults with psoriasis:A cross-sectional study 认领 引用 被引量：1
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作者 Lingquan Deng Yamei Gao +1 位作者 Chenxingyue Zhang Zhiqiang Yin 《Journal of Biomedical Research》 CAS CSCD 2026年第2期224-226,共3页
Dear Editor,Psoriasis,a chronic inflammatory cutaneous condition,is characterized by the development of red plaques with silvery scales,significantly affecting patients'quality of life and mental health[1].This co... Dear Editor,Psoriasis,a chronic inflammatory cutaneous condition,is characterized by the development of red plaques with silvery scales,significantly affecting patients'quality of life and mental health[1].This condition is thought to affect approximately 2%of the Western population,with diagnosis peaking in early adulthood[2].Vitamin D,a fat-soluble vitamin,is essential for phospho-calcium metabolism,calcium homeostasis,and bone health. 展开更多
关键词 serum oh d levels cancer cross sectional study inflammatory cutaneous conditionis psoriasis adults
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Effects of chronic administrations of aconitine on digestive tract and serum metabolism in mice 认领 引用
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作者 Siqian Chen Xianxian Jia +6 位作者 Meihui Cheng Wei Wang Chunling Ma Shujin Li Lili Ren Jianwei Wang Bin Cong 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第2期590-600,共11页
The objective of this study was to understand the effect of long-term aconitine(AC)oral administration on the digestive tract and serum metabolism.Subjects consumed either 0.9%Na Cl(n=8)or AC(n=17)gavage designed to r... The objective of this study was to understand the effect of long-term aconitine(AC)oral administration on the digestive tract and serum metabolism.Subjects consumed either 0.9%Na Cl(n=8)or AC(n=17)gavage designed to represent human chronic AC administrations for 13 days.Organ pathology was determined using hematoxylin-eosin staining and immunohistochemistry.Fecal and proximal intestinal content samples were collected to perform shotgun metagenomic sequencing.Serum samples were collected,and untargeted metabolomics was performed.In this study,AC administration induced proximal intestine,liver,and kidney injury.Microbiome composition remained stable after AC exposure,while several microbes presented dynamic alteration.Moreover,AC affected the abundance of the fatty acid biosynthesis rate-limiting gene acc A at day 7.AC induces 30 serum metabolites to significantly change at day 14,including several short-chain acylcarnitines.WGCNA revealed 2 sub-modules associated with the level of several short-chain acylcarnitines.In summary,AC affects the digestive tract and serum metabolism after chronic administration.AC may affect the enrichment of microbial-derived acc A gene.The abundance of serum acylcarnitines detected in the AC group may associate with its anti-heart failure effects. 展开更多
关键词 Aconitine Oral poison Microbiome Digestive tract Serum metabolism
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Untargeted metabolomic reveals changes in serum metabolism in peanut allergic mice treated by raw and roasted peanuts 认领 引用
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作者 Ying Zhang Jie Zhang +4 位作者 Xin Li Anshu Yang Ping Tong Zhihua Wu Hongbing Chen 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第4期1811-1822,共12页
Peanut allergy(PA),which affects an increasing proportion of people,is gaining increasing interests.Clarification of the mechanism underlying PA may aid in the identification of novel biomarkers and strategies for cli... Peanut allergy(PA),which affects an increasing proportion of people,is gaining increasing interests.Clarification of the mechanism underlying PA may aid in the identification of novel biomarkers and strategies for clinical intervention in allergic patients.In this study,raw peanut protein(Raw),roasted peanut protein(Roast),high-molecular-weight protein aggregation from Roast(OH),and the remaining components excluding OH from roasted peanut(OL)were administrated via intraperitoneal injection to challenge BALB/c mice.The allergic response was assessed,and the levels of peanut-specific immunoglobulin E,mouse mast cell protease-I,and T-lymphocyte subsets and secreted cytokines were detected.Then,serum untargeted metabolomics was investigated.Results show that Raw induced robust anaphylaxis in mice and up-or down-regulated 269 serum metabolites that mainly affected amino acid metabolism,including tryptophan metabolism,and induced disruption of tricarboxylic acid(TCA)cycle pathway in serum.Roast,OH and OL induced mild anaphylaxis in mice and mainly affected the glycerophospholipid metabolism in serum.Compared with Tris,OH upregulated the glycerophospholipid metabolism pathway in mouse serum,whereas Roast and OL showed the reversed effect.Mice in the Roast,OL,and OH groups all showed upregulated glycerophospholipid metabolism in serum compared with those in the Raw group.Meanwhile,different components of roasted peanut caused various changes in pathways when compared with each other.Compared with mice in the Roast group,those in the OL group displayed downregulated tryptophan and tyrosine metabolism,and those in the OH group showed upregulated glycerophospholipid metabolism and downregulated TCA cycle.The differences in serum metabolic profiles in mice implied that the processing form of allergen that patients are sensitized to and the severity of allergy should be considered in the analysis of serum biomarkers. 展开更多
关键词 Raw peanut Roasted peanut Allergenicity Serum metabolomics
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Bovine Serum Albumin over Graphene Oxide Surface:Binding Capacity and Interaction 认领 引用
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作者 RUPALI Pandey SANDEEP Singhania +3 位作者 AN JU RAGINI Chaturvedi RAJESH Chaudhary AMIT Garg 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS CSCD 2026年第4期1159-1165,共7页
We investigated the binding of universal protein BSA(Bovine Serum Albumin)over graphene oxide(GO)surface and performed quantitative measurements to find its maximum binding capacity.The interaction of proteins onto a ... We investigated the binding of universal protein BSA(Bovine Serum Albumin)over graphene oxide(GO)surface and performed quantitative measurements to find its maximum binding capacity.The interaction of proteins onto a 2D surface can happen via two available orthogonal extremes-side-on and end-on.Electrochemical measurements using cyclic voltammetry to define the uniqueness of the present study as it confirms the binding of BSA over GO surface and evaluate its maximum capacity.The reliability of the results is verified by repeating the experiment multiple times over a year.UV-Vis,FTIR,Raman,and SEM measurements clearly show that BSA is perfectly bound over GO.The estimated value of BSA over GO calculated by standard protein estimation test-Lowry’s protein assay is 1.1 mg/mg. 展开更多
关键词 Graphene oxide Bovine Serum Albumin Binding capacity Lowry’s protein assay Cyclic voltammetry
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Broad-spectrum and eicosanoid-targeted serum lipidomics reveal the therapeutic mechanism of moxibustion in a rat model of collagen-induced arthritis 认领 引用
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作者 Ying QIU Jia-tian MA +2 位作者 Yun FENG Jia-min WEN Zhi-ling SUN 《World Journal of Acupuncture-Moxibustion》 CAS CSCD 2026年第2期215-230,共16页
Objective Rheumatoid arthritis(RA)is a chronic autoimmune disease characterized by progressive joint destruction and high disability rates.Moxibustion,a traditional Chinese medicine therapy with a long history of use ... Objective Rheumatoid arthritis(RA)is a chronic autoimmune disease characterized by progressive joint destruction and high disability rates.Moxibustion,a traditional Chinese medicine therapy with a long history of use in RA management,has attracted increasing clinical attention.Although previous studies have explored the mechanisms underlying the efficacy of moxibustion in RA,its lipidomic mechanisms remain unclear.Therefore,this study aimed to elucidate the lipidomic mechanisms of moxibustion by integrating broad-spectrum targeted lipidomics and eicosanoid-targeted lipidomics to identify key lipid metabolites associated with its therapeutic effects.Methods Thirty-two male Sprague–Dawley rats were randomly assigned to four groups(n=8 per group):control,model,moxibustion control,and moxibustion model groups.The collagen-induced arthritis(CIA)model was established by two rounds of immunization.Moxibustion was applied bilaterally at“Zusanli(ST 36)”and“Shenshu(BL23)”for 10 min per acupoint(40 min per session).Treatment was administered once daily for 6 consecutive days per cycle,for three cycles,with a 1-day interval between cycles.After 3 weeks of treatment,therapeutic effects were evaluated based on body weight,paw volume,arthritis index(AI)score,visceral indices,and histopathological examination.Serum lipid profiles were analyzed to identify differential metabolites and metabolic pathways associated with moxibustion.Results Compared with the control group,rats in the model group exhibited poor general condition,significant body weight loss,and marked increases in paw volume and AI score(P<0.05),as well as significantly elevated visceral indices(P<0.05).In contrast,moxibustion treatment significantly improved general condition,restored body weight,and reduced paw volume,AI score,and visceral indices compared with the model group(P<0.05).Histopathological examination revealed marked synovial hyperplasia,inflammatory cell infiltration,and cartilage destruction in the ankle joints of the model group,whereas these pathological changes were significantly alleviated by moxibustion.Lipidomic analysis identified 81 differential metabolites between the model and control groups,and moxibustion ameliorated disruptions in the arachidonic acid(ARA)and linoleic acid(LA)pathways.Broad-spectrum targeted lipidomics showed that moxibustion regulated 19 lipid species.Eicosanoid-targeted profiling further demonstrated that,under pathological conditions,moxibustion modulated eight of these lipids to attenuate proinflammatory mediators,whereas under physiological conditions,it adjusted six lipids to maintain lipid homeostasis.Conclusion CIA rats exhibited significant disturbances in lipid metabolism,mainly involving the ARA and LA pathways.Moxibustion effectively regulated broad-spectrum lipid classes and specific eicosanoid mediators,thereby alleviating inflammation under pathological conditions and contributing to physiological homeostasis. 展开更多
关键词 Lipidomics Rheumatoid arthritis Inflammatory mediators Serum biomarkers Traditional Chinese medicine(TCM)therapy Metabolic pathways
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Mac-2 binding protein glycosylation isomer as a novel serum biomarker for recurrence in hepatocellular carcinoma 认领 引用
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作者 Kyle R Stephens Megan Wilson +3 位作者 Manting Xu Jeremy T Gaskins Gina Genova Robert C G Martin II 《World Journal of Gastroenterology》 SCIE CAS 2026年第5期148-156,共9页
BACKGROUND Accurate prediction of hepatocellular carcinoma(HCC)recurrence after curative therapy remains challenging.Mac-2 binding protein glycosylation isomer(M2-BPGi),a serum marker of liver fibrosis,may serve as a ... BACKGROUND Accurate prediction of hepatocellular carcinoma(HCC)recurrence after curative therapy remains challenging.Mac-2 binding protein glycosylation isomer(M2-BPGi),a serum marker of liver fibrosis,may serve as a noninvasive prognostic biomarker.AIM To evaluate the association between preoperative M2BPGi levels and HCC recurrence following curative treatment.METHODS We searched PubMed Cochrane CENTRAL,EMBASE,and BIOSIS Citation Index for English-language studies in humans reporting HCC recurrence outcomes stratified by high vs low serum M2BPGi.Four retrospective studies(total n=494)met inclusion criteria for meta-analysis.Data on recurrence-related survival(recurrence-free,tumor-free,or progression-free survival)and unadjusted and/or adjusted hazard ratios(HR)for high vs low M2BPGi were extracted.Randomeffects meta-analyses were performed separately for univariate and multivariate HRs.Heterogeneity was assessed by I2 and publication bias by Egger’s test.RESULTS High preoperative M2BPGi was significantly associated with increased recurrence risk.The pooled unadjusted HR was 2.98(95%CI:1.50-5.91;P<0.01;I2=38.3%),and the pooled adjusted HR was 2.22(95%CI:1.48-3.32;P<0.01;I2=0%).Meta-regression showed no effect of varying cutoff thresholds on HRs,and Egger’s tests indicated no evidence of publication bias in the multivariate results.The bias-corrected estimated of the univariate HR remained statistically significant(HR=2.31,95%CI:1.21-4.41,P=0.021,I2=32%).CONCLUSION Preoperative serum M2BPGi is a promising biomarker for HCC recurrence after hepatectomy.Its strong association with risk,minimal heterogeneity across studies,and ease of measurement support its potential clinical utility. 展开更多
关键词 Hepatocellular carcinoma Biomarker Serum Recurrence Mac-2 binding protein glycosylation isomer
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Threshold effects of serum 25-hydroxyvitamin D levels on major depressive symptoms 认领 引用
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作者 Qinghua Fan Yi Guo +2 位作者 Zhongjian Liu Rongtian Xu Haibo Wang 《General Psychiatry》 CAS CSCD 2026年第1期71-75,共5页
To the Editor:Depression is a major contributor to the global burden of disease and disability,posing a critical public health challenge.Meta-analyses have demonstrated that vitamin D supplementation significantly red... To the Editor:Depression is a major contributor to the global burden of disease and disability,posing a critical public health challenge.Meta-analyses have demonstrated that vitamin D supplementation significantly reduces major depressive symptoms(MDS).1 However,recent large-scale randomised controlled trials(RCTs)2-4 have yielded conflicting evidence,indicating that vitamin D supplementation may not necessarily reduce MDS risk.This discrepancy may be attributed to variations in baseline serum 25-hydroxyvitamin D(25(OH)D)levels,insufficiently characterised doseresponse relationships and uncertain optimal 25(OH)D thresholds. 展开更多
关键词 threshold effects serum hydroxyvitamin D levels major depressive symptoms randomised controlled trials rcts vitamin d major depressive symptoms mds
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Huangqin decoction ameliorates ulcerative colitis by regulating ferroptosis based on the integrative analysis of serum pharmaco-chemistry with metabolomics and network pharmacology 认领 引用
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作者 Jia-Yao Xiong Xin-Yue Ji +5 位作者 Chong-Bo Zhao Liu Yang Jia-Hui Zheng Ya-Jun Shi Jing Wang Min Wang 《Traditional Medicine Research》 2026年第10期20-33,共14页
Background:Huangqin decoction(HQD),a classic formula for treating ulcerative colitis(UC),exhibits anti-inflammatory and intestinal mucosa protective effects.However,its mechanisms require further investigation.This st... Background:Huangqin decoction(HQD),a classic formula for treating ulcerative colitis(UC),exhibits anti-inflammatory and intestinal mucosa protective effects.However,its mechanisms require further investigation.This study aimed to elucidate these mechanisms by integrating metabolomics and serum pharmacochemistry-based network pharmacology.Methods:A dextran sulfate sodium(DSS)-induced mice model of UC was established to assess the therapeutic effect of HQD.Chemical compounds and absorbed constituents of HQD were identified by UHPLC-Q-Orbitrap-MS.Network pharmacology predicted targets and pathways based on blood-absorbed constituents.Differential metabolites and associated pathways were identified by serum metabolomics.Fe2+and GSH levels in colon tissues were measured to assess ferroptosis.Molecular docking evaluated binding affinities,and the expression of ferroptosis-related targets was validated by RT-qPCR and Western blot.Results:A total of 92 chemical compounds and 66 blood-absorbed constituents were identified.Compared to the DSS group,44 differential metabolites were reversed in the HQD group,enriched in tryptophan metabolism,arginine and proline metabolism,and pyrimidine metabolism.Joint analysis of network pharmacology and metabolomics focused on the arachidonic acid pathway and ferroptosis-related targets.HQD inhibited ferroptosis,evidenced by decreased Fe2+levels and restored GSH content.RT-qPCR,Western blotting,and molecular docking demonstrated that HQD bioactive components exhibited significant binding affinity for and regulatory activity toward four key ferroptosis-related targets:PTGS2,ALOX5,GPX4,and STAT3.Conclusion:The integrated analysis strategy suggests that the efficacy of HQD against UC may be associated with its regulation of inflammatory responses and ferroptosis-related targets,providing a preliminary basis for elucidating the material basis and mechanisms of HQD. 展开更多
关键词 Huangqin decoction ulcerative colitis serum pharmacochemistry metabolomics ferroptosis
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Diagnostic and prognostic utility of serum bile acid in liver transplant recipients:A systematic review and quantitative synthesis 认领 引用
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作者 Eyad Gadour Hadi Kuriry +3 位作者 Bogdan Miutescu Bodour Raheem Syed A Gardezi Mohammed S AlQahtani 《World Journal of Hepatology》 2026年第5期273-280,共8页
BACKGROUND Liver transplantation(LT)remains the definitive treatment for patients with acute and chronic end-stage liver disease,significantly improving survival and quality of life.Conventional liver function tests p... BACKGROUND Liver transplantation(LT)remains the definitive treatment for patients with acute and chronic end-stage liver disease,significantly improving survival and quality of life.Conventional liver function tests post LT,while routinely used,often lack sensitivity and may not detect graft injury promptly.Serum bile acids(SBAs),known for their role in hepatic function and enterohepatic circulation,have emerged as promising biomarkers due to their sensitivity to hepatocellular injury and cholestasis.This systematic review evaluates the prognostic value of SBA levels following LT.AIM To assess correlations between SBA concentrations and clinical outcomes,including graft function and rejection episodes.METHODS Relevant studies analyzing SBA levels after LT were systematically reviewed.Data synthesis focused on the association between bile acid levels and transplant outcomes.RESULTS Elevated SBA levels were significantly associated with early graft dysfunction and acute rejection.Pooled analysis indicated that patients with SBA levels exceeding study-specific thresholds had a 2.5-fold increased risk of acute rejection(95%CI:1.8-3.4,P<0.001).Diagnostic accuracy analysis showed that SBA levels had a sensitivity of 82%and specificity of 76%for predicting graft dysfunction within the first month after transplantation.Subgroup analyses highlighted conjugated bile acids as particularly predictive,with an odds ratio of 3.1(95%CI:2.0-4.8,P<0.0001)for adverse outcomes.CONCLUSION SBA levels demonstrate strong potential as a non-invasive early biomarker for post-liver transplant prognosis,facilitating timely clinical interventions.However,heterogeneity in assay techniques and cutoff values across studies underscores the need for standardized measurement protocols.Incorporating SBA monitoring into routine post-transplant surveillance may enhance prognostic precision and improve patient management strategies. 展开更多
关键词 Serum bile acid Liver transplantation Surveillance strategies Graft dysfunction Graft rejection Biliary complications
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Serum HMGB1 is a potential biomarker for artificial liver treatment in patients with acute liver failure 认领 引用
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作者 Qiu-Yan Zhao Shu-Jing Liu +12 位作者 Zan Zuo Zuo-Yong Li Juan Xu Chun-Ping Yin Hong-Na Li Li-Na Zhu Chun-Fang Li Hui-Ming Zhang Lin-Ling Qian Jia-Long Qi Zheng-Ji Song Dan-Rong Ni Yuan Tang 《Life Research》 2026年第2期22-30,共9页
Background:High-mobility group box 1(HMGB1)is a critical damage-associated molecular pattern protein that participates in diverse physiological and pathological processes.However,its relevance to the prognosis of arti... Background:High-mobility group box 1(HMGB1)is a critical damage-associated molecular pattern protein that participates in diverse physiological and pathological processes.However,its relevance to the prognosis of artificial liver support therapy in patients with acute liver injury(ALF)remains unclear.Methods:Bioinformatics analyses were performed to identify HMGB1-interacting proteins and associated inflammatory signaling pathways.Peripheral blood samples were collected from ALF patients before and after artificial liver support therapy,and serum HMGB1 concentrations were quantified using ELISA.Primary mouse hepatocytes were stimulated with lipopolysaccharide(LPS)in vitro and HMGB1 expression was verified by western blot.Results:Single-cell transcriptomic profiling showed that HMGB1 is widely expressed across tissues and predominantly localized in the nucleus.In the liver,HMGB1 was primarily expressed in hepatocytes and hepatic stellate cells.STRING database analysis revealed that human HMGB1 interacts with multiple proteins,including TLR4,TP53,and BECN1.The constructed interaction network comprised 11 nodes with an average local clustering coefficient of 0.888,and the protein–protein interaction enrichment P-value was 1.42×10-5,indicating significant enrichment.Gene Ontology and KEGG pathway enrichment analyses demonstrated that HMGB1 is closely linked to inflammatory and injury-related signaling pathways,including the TLR and NLR pathways.Metabolomic profiling revealed significant metabolic alterations between patients with ALF and healthy controls under both positive and negative ion modes and functional analysis showed necroptosis was activated.The cell viability gradually decreased with time and dose under LPS treatment and extracellular HMGB1 was upregulated in LPS induced ALF model and patients(P<0.05).Serum HMGB1/RIPK3/MLKL levels were markedly elevated in ALF patients compared with controls(P<0.05)and progressively declined following artificial liver support therapy.Furthermore,elevated HMGB1 concentrations were positively correlated with unfavorable clinical outcomes.Conclusion:Peripheral blood HMGB1 levels are significantly increased in patients with acute liver failure,decrease following artificial liver support therapy,and are positively associated with poor clinical prognosis. 展开更多
关键词 artificial liver treatment acute liver failure serum HMGB1 biomarker prognosis
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