Suillus granulatus is a valuable wild edible mushroom with a strong umami taste.Different grades of S.granulatus were dried by different methods,including natural air drying(ND),hot air drying(HAD),vacuum drying(VD),a...Suillus granulatus is a valuable wild edible mushroom with a strong umami taste.Different grades of S.granulatus were dried by different methods,including natural air drying(ND),hot air drying(HAD),vacuum drying(VD),and freeze drying(FD).Results showed that VD samples of all grades had the highest equivalent umami concentration(EUC)value(P<0.05).The second grade of ND samples showed the highest EUC value(P<0.05).The first grade of HAD samples showed the highest EUC value(P<0.05).The third grade of FD samples showed the highest EUC value(P<0.05).The third grade of VD samples had a higher EUC value than the other grades of samples dried by VD.Electronic tongue results indicated no significant differences between samples of all grades dried by all methods.Thus,umami taste components are affected by drying method and grade.VD is an appropriate drying method for all sample grades.ND,HAD,and FD are suitable for second-,first-,and third-grade samples,respectively.展开更多
Concentration of copper and zinc in isolated Suillus bovinus mycelia, used nutrient solution and 0.5 mol/L EDTA mycelia washing solution were measured to investigate the distribution of heavy metals in mycelia growth ...Concentration of copper and zinc in isolated Suillus bovinus mycelia, used nutrient solution and 0.5 mol/L EDTA mycelia washing solution were measured to investigate the distribution of heavy metals in mycelia growth in excess copper or zinc nutrient solution. Treated with zinc, most of added zinc maintained in used solution, and 9.8%/14.6% was in/on mycelia in treatment, and in treatment 2 was 3.9%/8.0% in/on mycelia. In the copper applications, copper stimulated in more than on mycelia, i.e., 25.9%/4.5% in/on mycelia in treatment, and 7%/18.8% in/on mycelia while most of copper retained in used nutrient solution. Certain amount of copper or zinc uptake by mycelia led to pronounced influence on glycolysis and nitrogen incorporating process of Suillus bovinus, while the tested enzymes kept constant in treatment. In crude extracts of copper treatment 2 mycelia, activities of HK, PFK and GS were inhibited and decrease to 63%, 48% and 38% and GIDH increased by 68% of the control, respectively. The behaviors of these tested enzymes toward zinc corresponded in general with that towards copper. The potential protection of Suillus bovinus for its host plant under excess copper or zinc threaten was discussed.展开更多
A large areas of Larch seedlings, including container and bare-root seedlings (new and transplanted seedlings ) were inoculated pure inocula that were cultivated by using Suillus grevillei. Compared with those of cont...A large areas of Larch seedlings, including container and bare-root seedlings (new and transplanted seedlings ) were inoculated pure inocula that were cultivated by using Suillus grevillei. Compared with those of control area, all growth indices as rate of emergence, growth(in seedling height, collar diameter and number of lateral branch-root), rate of mycorrhiza inoculum, rate of survival and content of nutrient elements were greatly increased. Therefore, Suillus grevillei is a better and suitable mycorrhizal fungus to larch seedling either in the severe cold mountain area or in the plain region. It should be spread and used in larch cultivation and planting.展开更多
In this study,pot experiments were conducted on the seedlings of Pinus sylvestris var.mongolica to study the influence of Trichoderma(Trichoderma harzianum E15)and Ectomycorrhizal fungi(Suillus luteus N94)on the growt...In this study,pot experiments were conducted on the seedlings of Pinus sylvestris var.mongolica to study the influence of Trichoderma(Trichoderma harzianum E15)and Ectomycorrhizal fungi(Suillus luteus N94)on the growth of these seedlings.In particular,the effects of these fungi on the fungal community structure in the rhizosphere soil of the seedlings were investigated.Inoculation with Trichoderma harzianum E15 and Suillus luteus N94 significantly(P<0.05)promoted the growth of the Pinus sylvestris seedlings.The non-metric multidimensional scaling(NMDS)results indicated a significant difference(P<0.05)between the fungal community structures in the rhizosphere soil of the annual and biennial seedlings.In the rhizosphere soil of annual seedlings,the main fungi were Ascomycota,Basidiomycota,Zygomycota.Ascomycota,Basidiomycota,Mortierellomycota,and p-unclassified-k-Fungi were the main fungi in the rhizosphere soil of biennial seedlings.The dominant genus in the rhizosphere soil and a key factor promoting the growth of the annual and the biennial seedlings was Trichoderma,Suillus,respectively.Both of them were negatively correlated with the relative abundance of microbial flora in the symbiotic environment.Trichoderma had a significant promoting effect on the conversion of total phosphorus,total nitrogen,ammonium nitrogen,nitrate nitrogen,and the organic matter in the rhizosphere soil of the seedlings,while Suillus significantly promoted the conversion of organic matter and total phosphorus.展开更多
Mongolian pine is an important afforestation species widely used for ecological management in northeast China. The environment in this region is very unstable and the flora are regularly subjected to drought stress.Th...Mongolian pine is an important afforestation species widely used for ecological management in northeast China. The environment in this region is very unstable and the flora are regularly subjected to drought stress.This paper reports on the influence of inoculation with the Suillus luteus on seedlings under different water conditions.Both inoculated and non-inoculated ectomycorrhizal fungi(ECMF)-S. luteus seedlings were maintained under wellwatered or water-stress conditions for 3 months. The S.luteus colonization rate under water stress was higher than that in well-watered conditions. Under water stress, inoculated seedlings had greater growth than non-inoculated seedlings. In addition, under water stress, S. luteus-inoculated seedlings had greater superoxide dismutase and peroxidase activity, higher soluble protein content, lower proline content, and lower malondialdehyde content than non-inoculated seedlings. S. luteus colonization increased the rhizosphere soil-enzyme activity and the rhizosphere soil nutrition content under both well-watered and water-stress conditions. Given the positive impact on seedling growth and physiology, S. luteus shows potential for use in the arid and semi-arid regions of northeast China for afforestation.展开更多
Korean spruce (Picea koraiensis Sieb. E1 Zucc.) is one of the main afforestation species in northern China. Seedling quality is a critical factor at planting time. To test whether the synergistic growth enhancement ...Korean spruce (Picea koraiensis Sieb. E1 Zucc.) is one of the main afforestation species in northern China. Seedling quality is a critical factor at planting time. To test whether the synergistic growth enhancement of Scotch pine (P. sylvestris var. mongolica) seedlings brought by the plant beneficial fungus Trichoderma virens (J.H. Mill., Giddens and A.A. Foster) Arx and ectomycorrhizal fungus (Suillus luteus (L.) Roussel.) can also benefit Korean spruce seed- lings, we examined the effects of S. luteus and T. virens on the growth of P. koraiensis seedlings and drought resistance of P. sylvestris var. mongolica in peat soils. The two fungi were added to sterilized peat soil in pots, and the plants were grown for 4 months. Seedling growth and physiological variables, including mycorrhizal colonization rate of roots, biomass, and chlorophyll content, were examined. The colonization rate of the mycorrhizal fungus on P. koraiensis exceeded 65 %, and the synergism between S. luteus and T. virens enhanced most of the variables for P. koraiensis seedlings after inoculation with S. luteus then 30 days later with T. virens as in our published results for seedlings of P. sylvestris var. mongolica. When seedlings of P. sylvestris var. mongolica were inoculated with this sequence, they became more drought tolerant. T. virens also induced S. luteus to produce -l,3-glucanase and chitinase. This inocu- lation sequence at planting can thus improve the quality of P. sylvestris var. mongolica and P. koraiensis seedlings and substantiates our previous results.展开更多
The addition of ectomycorrhizal fungi(ECMF),beneficial rhizosphere microorganisms,to the soil can pro-mote plant growth and resistance.Here,Populus davidi-ana×Populus bolleana tissue culture seedlings were grown ...The addition of ectomycorrhizal fungi(ECMF),beneficial rhizosphere microorganisms,to the soil can pro-mote plant growth and resistance.Here,Populus davidi-ana×Populus bolleana tissue culture seedlings were grown for 3 months in soils inoculated with one of the species,then seedlings were assessed for mycorrhizal colonization rate and growth,physiological and root traits.Suillus luteus and Populus involutus each formed ectomycorrhizal associa-tions with the seedlings.Seedling height,ground diameter,biomass,and leaf area were significantly greater after treat-ment with ECMF than in the non-inoculated controls.Treat-ment improved all physiological and root variables assessed(chlorophylls and carotenoids,cellulose,and soluble sugars and proteins;root length,surface area,projected area,mean diameter,volume,number of root tips).Seedlings inocu-lated with S.luteus outperformed those inoculated with P.involutus.展开更多
基金Liaoning Provincial Department of Education Annual(2019)Scientific Research Fund Project(No.LSNZD201903)National Key R&D Program of China(No.2018YFD0400200)+1 种基金Liaoning Province,Shenyang Agricultural University,High-end Talent Introduction Fund Project(NO.SYAU20160003)Natural Science Foundation of Liaoning Province(No.20170540822).
摘要Suillus granulatus is a valuable wild edible mushroom with a strong umami taste.Different grades of S.granulatus were dried by different methods,including natural air drying(ND),hot air drying(HAD),vacuum drying(VD),and freeze drying(FD).Results showed that VD samples of all grades had the highest equivalent umami concentration(EUC)value(P<0.05).The second grade of ND samples showed the highest EUC value(P<0.05).The first grade of HAD samples showed the highest EUC value(P<0.05).The third grade of FD samples showed the highest EUC value(P<0.05).The third grade of VD samples had a higher EUC value than the other grades of samples dried by VD.Electronic tongue results indicated no significant differences between samples of all grades dried by all methods.Thus,umami taste components are affected by drying method and grade.VD is an appropriate drying method for all sample grades.ND,HAD,and FD are suitable for second-,first-,and third-grade samples,respectively.
摘要Concentration of copper and zinc in isolated Suillus bovinus mycelia, used nutrient solution and 0.5 mol/L EDTA mycelia washing solution were measured to investigate the distribution of heavy metals in mycelia growth in excess copper or zinc nutrient solution. Treated with zinc, most of added zinc maintained in used solution, and 9.8%/14.6% was in/on mycelia in treatment, and in treatment 2 was 3.9%/8.0% in/on mycelia. In the copper applications, copper stimulated in more than on mycelia, i.e., 25.9%/4.5% in/on mycelia in treatment, and 7%/18.8% in/on mycelia while most of copper retained in used nutrient solution. Certain amount of copper or zinc uptake by mycelia led to pronounced influence on glycolysis and nitrogen incorporating process of Suillus bovinus, while the tested enzymes kept constant in treatment. In crude extracts of copper treatment 2 mycelia, activities of HK, PFK and GS were inhibited and decrease to 63%, 48% and 38% and GIDH increased by 68% of the control, respectively. The behaviors of these tested enzymes toward zinc corresponded in general with that towards copper. The potential protection of Suillus bovinus for its host plant under excess copper or zinc threaten was discussed.
摘要A large areas of Larch seedlings, including container and bare-root seedlings (new and transplanted seedlings ) were inoculated pure inocula that were cultivated by using Suillus grevillei. Compared with those of control area, all growth indices as rate of emergence, growth(in seedling height, collar diameter and number of lateral branch-root), rate of mycorrhiza inoculum, rate of survival and content of nutrient elements were greatly increased. Therefore, Suillus grevillei is a better and suitable mycorrhizal fungus to larch seedling either in the severe cold mountain area or in the plain region. It should be spread and used in larch cultivation and planting.
摘要In this study,pot experiments were conducted on the seedlings of Pinus sylvestris var.mongolica to study the influence of Trichoderma(Trichoderma harzianum E15)and Ectomycorrhizal fungi(Suillus luteus N94)on the growth of these seedlings.In particular,the effects of these fungi on the fungal community structure in the rhizosphere soil of the seedlings were investigated.Inoculation with Trichoderma harzianum E15 and Suillus luteus N94 significantly(P<0.05)promoted the growth of the Pinus sylvestris seedlings.The non-metric multidimensional scaling(NMDS)results indicated a significant difference(P<0.05)between the fungal community structures in the rhizosphere soil of the annual and biennial seedlings.In the rhizosphere soil of annual seedlings,the main fungi were Ascomycota,Basidiomycota,Zygomycota.Ascomycota,Basidiomycota,Mortierellomycota,and p-unclassified-k-Fungi were the main fungi in the rhizosphere soil of biennial seedlings.The dominant genus in the rhizosphere soil and a key factor promoting the growth of the annual and the biennial seedlings was Trichoderma,Suillus,respectively.Both of them were negatively correlated with the relative abundance of microbial flora in the symbiotic environment.Trichoderma had a significant promoting effect on the conversion of total phosphorus,total nitrogen,ammonium nitrogen,nitrate nitrogen,and the organic matter in the rhizosphere soil of the seedlings,while Suillus significantly promoted the conversion of organic matter and total phosphorus.
基金supported by the National Natural Science Foundation of China(31670649,31200484,31170597)
摘要Mongolian pine is an important afforestation species widely used for ecological management in northeast China. The environment in this region is very unstable and the flora are regularly subjected to drought stress.This paper reports on the influence of inoculation with the Suillus luteus on seedlings under different water conditions.Both inoculated and non-inoculated ectomycorrhizal fungi(ECMF)-S. luteus seedlings were maintained under wellwatered or water-stress conditions for 3 months. The S.luteus colonization rate under water stress was higher than that in well-watered conditions. Under water stress, inoculated seedlings had greater growth than non-inoculated seedlings. In addition, under water stress, S. luteus-inoculated seedlings had greater superoxide dismutase and peroxidase activity, higher soluble protein content, lower proline content, and lower malondialdehyde content than non-inoculated seedlings. S. luteus colonization increased the rhizosphere soil-enzyme activity and the rhizosphere soil nutrition content under both well-watered and water-stress conditions. Given the positive impact on seedling growth and physiology, S. luteus shows potential for use in the arid and semi-arid regions of northeast China for afforestation.
基金the National Natural Science Foundation of China (31170597, 31200484)the National Fiveyear Science and Technology Research Project (2012BAD19B0801)+1 种基金the Fundamental Research Funds for the Central Universities (2572014AA30)the National Forestry Bureau ‘‘948’’ Project (2009-4-39) for financial support
摘要Korean spruce (Picea koraiensis Sieb. E1 Zucc.) is one of the main afforestation species in northern China. Seedling quality is a critical factor at planting time. To test whether the synergistic growth enhancement of Scotch pine (P. sylvestris var. mongolica) seedlings brought by the plant beneficial fungus Trichoderma virens (J.H. Mill., Giddens and A.A. Foster) Arx and ectomycorrhizal fungus (Suillus luteus (L.) Roussel.) can also benefit Korean spruce seed- lings, we examined the effects of S. luteus and T. virens on the growth of P. koraiensis seedlings and drought resistance of P. sylvestris var. mongolica in peat soils. The two fungi were added to sterilized peat soil in pots, and the plants were grown for 4 months. Seedling growth and physiological variables, including mycorrhizal colonization rate of roots, biomass, and chlorophyll content, were examined. The colonization rate of the mycorrhizal fungus on P. koraiensis exceeded 65 %, and the synergism between S. luteus and T. virens enhanced most of the variables for P. koraiensis seedlings after inoculation with S. luteus then 30 days later with T. virens as in our published results for seedlings of P. sylvestris var. mongolica. When seedlings of P. sylvestris var. mongolica were inoculated with this sequence, they became more drought tolerant. T. virens also induced S. luteus to produce -l,3-glucanase and chitinase. This inocu- lation sequence at planting can thus improve the quality of P. sylvestris var. mongolica and P. koraiensis seedlings and substantiates our previous results.
基金part of the Liaoning Provincial Department of Education project LJKZ0684supported by the National Natural Science Foundation of China (31800542)
摘要The addition of ectomycorrhizal fungi(ECMF),beneficial rhizosphere microorganisms,to the soil can pro-mote plant growth and resistance.Here,Populus davidi-ana×Populus bolleana tissue culture seedlings were grown for 3 months in soils inoculated with one of the species,then seedlings were assessed for mycorrhizal colonization rate and growth,physiological and root traits.Suillus luteus and Populus involutus each formed ectomycorrhizal associa-tions with the seedlings.Seedling height,ground diameter,biomass,and leaf area were significantly greater after treat-ment with ECMF than in the non-inoculated controls.Treat-ment improved all physiological and root variables assessed(chlorophylls and carotenoids,cellulose,and soluble sugars and proteins;root length,surface area,projected area,mean diameter,volume,number of root tips).Seedlings inocu-lated with S.luteus outperformed those inoculated with P.involutus.