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Two-component signaling system RegAB represses Pseudomonas syringae pv. actinidiae T3SS by directly binding to the promoter of hrpRS 认领 引用
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作者 Mengsi Zhang Mingming Yang +7 位作者 Xiaoxue Zhang Shuying Li Shuaiwu Wang Alex Muremi Fulano Yongting Meng Xihui Shen Lili Huang Yao Wang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2026年第5期1992-2002,共11页
Kiwifruit bacterial canker,caused by Pseudomonas syringae pv.actinidiae(Psa),is a significant threat to the kiwifruit industry.The two-component signaling systems(TCSs) play a crucial role in regulating the virulence ... Kiwifruit bacterial canker,caused by Pseudomonas syringae pv.actinidiae(Psa),is a significant threat to the kiwifruit industry.The two-component signaling systems(TCSs) play a crucial role in regulating the virulence of P.syringae,yet their specific function in Psa remains largely unclear.In this study,we found that disrupting the TCS RegAB(encoded by Psa 802/Psa_ 803) resulted in a notable increase in the virulence of P.syringae pv.actinidiae M228(Psa M228) in host plant and hypersensitive reaction(HR) in nonhost plant.Through comparative transcriptome analysis of the Psa M228wild-type strain and the regA mutant,we identified the pivotal role of RegAB in controlling various physiological pathways,including the type Ⅲ secretion system(T3SS),a key determinant of Psa virulence.Additionally,we discovered that the RegA has binding sites in the promoter region of the hrpR/S,and the transcriptional level of the hrpR and other T3SSrelated genes increased in the regA deletion strain relative to the Psa M228 wild-type.The DNA-binding affinity of RegA,and therefore the repressor function,is enhanced by its phosphorylation.Our findings unveil the function of TCS RegAB and the regulatory mechanism of T3SS by RegAB in Psa,highlighting the diverse functions of the RegAB system. 展开更多
关键词 Pseudomonas syringae pv.actinidiae typeⅢsecretion system(T3SS) two-component signaling system RegAB hrpR/S
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Vascular network-mediated systemic spread of Pseudomonas syringae pv.actinidiae causes the bacterial canker of kiwifruit 认领 引用 被引量：3
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作者 Runze Tian Yujie Tian +2 位作者 Qianqian Dang Hongchang Zhang Lili Huang 《Horticultural Plant Journal》 SCIE CAS CSCD 2025年第6期2093-2108,共16页
Pseudomonas syringae pv.actinidiae(Psa)causes destructive kiwifruit bacterial canker by invading vascular tissues across multiple plant organs.However,the cellular mechanism underlying its systemic transmission and ce... Pseudomonas syringae pv.actinidiae(Psa)causes destructive kiwifruit bacterial canker by invading vascular tissues across multiple plant organs.However,the cellular mechanism underlying its systemic transmission and cell-to-cell movement within these specialized vascular conduits remains unclear.In this study,a Psa-GFP strain and various microscopic techniques were used to investigate the interaction between kiwifruit and Psa.Our results reveal that Psa strategically exploits host vascular conduits for systemic movement,with the xylem vessel being the predominant avenue.In the phloem,Psa exhibits adaptive alteration in bacterial shape to traverse sieve pores,facilitating its systemic spread along sieve tubes and inducing phloem necrosis.Within the xylem,Psa breaches pit membranes to migrate between adjacent vessels.Furthermore,phloem fibers act as an initial barrier at the early stages of infection,delaying Psa's entry into vascular tissues during its journey to the xylem.Additionally,at the junctions of stem-stem or stem-leaf,branch trace or leaf trace mediates the bacterial organ-to-organ translocation,thus facilitating the systemic progression of disease.In conclusion,our findings shed light on the cellular mechanism employed by Psa to exploit the woody plant's vascular network for infection,thereby enhancing a better understanding of the biology of this poorly defined bacterium.These insights carry implications for the pathogenesis of Psa and other vascular pathogens,offering theoretical guidance for effective control strategies. 展开更多
关键词 Pseudomonas syringae pv.actinidiae Kiwifruit Sieve pore Pit membrane Systemic transmission
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The E3 ubiquitin ligase SlATL2 suppresses tomato immunity by promoting SlcsN5a degradation during Pseudomonas syringae pv.tomato DC3000 infection 认领 引用
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作者 Yujie Dai Xiaodan Li +4 位作者 Yeling He Liya Zhu Yan Bi Fengming Song Dayong Li 《Horticulture Research》 SCIE CSCD 2025年第6期339-354,共16页
Plant immunity involves complex regulatory mechanisms that mediate the activation of defense responses against pathogens.Protein degradation via ubiquitination plays a crucial role in modulating these defenses,with E3... Plant immunity involves complex regulatory mechanisms that mediate the activation of defense responses against pathogens.Protein degradation via ubiquitination plays a crucial role in modulating these defenses,with E3 ubiquitin ligases functioning as central regulators.This study investigates the role of SlATL2,an ARABIDOPSIS TÓXICOS EN LEVADURA(ATL)-type E3 ubiquitin ligase localized in the plasma membrane,in the immune response of tomato plants against Pseudomonas syringae pv.tomato(Pst)DC3000.Our findings demonstrate that SlATL2 expression is induced upon Pst DC3000 infection and treatment with defense hormones salicylic acid and jasmonic acid.Functionally,SlATL2 negatively regulates immune responses,impairing resistance to Pst DC3000 and suppressing flg22-triggered immunity.In addition,SlATL2 limits pathogen-induced reactive oxygen species and callose accumulation by targeting the COP9 signalosome subunit 5a(SlCSN5a),a key positive regulator of tomato defense responses against Pst DC3000.This interaction,which occurs via the N-terminal residue of SlATL2,results in the ubiquitination and 26S proteasomal degradation of SlCSN5a,thereby suppressing SA-dependent expression of defense response genes associated and limiting reactive oxygen species production.This work sheds light on the molecular mechanism through which the E3 ubiquitin ligase SlATL2 attenuates tomato immune responses by targeting a Cop9 signalosome subunit for degradation.These discoveries deepen our insights into the post-translational mechanisms governing plant immune responses and provide fresh opportunities to bolster crop resistance against bacterial pathogens. 展开更多
关键词 e ubiquitin ligases pseudomonas syringae regulatory mechanisms immune response tomato plants plant immunity plasma membranein
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Syringa pubescens Turcz.ethyl acetate extract improves non-alcoholic steatohepatitis by regulating the Nrf2,IκBαand TGF-β1 signaling pathways 认领 引用
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作者 Weidong Xu Yanfang Wu +7 位作者 Jiansheng Xiong Jiameng Zhang Zichen Zhang Xinyi Guan Suya Zhang Xinsheng Wang Pu Liu Enguo Fan 《Food Science and Human Wellness》 SCIE CAS CSCD 2025年第10期4074-4090,共17页
The flower of Syringa pubescens Turcz.(SP),was used as both medicine and food in China,exhibited various biological activities.However,it remains unknown whether SP affects ameliorative nonalcoholic steatohepatitis(NA... The flower of Syringa pubescens Turcz.(SP),was used as both medicine and food in China,exhibited various biological activities.However,it remains unknown whether SP affects ameliorative nonalcoholic steatohepatitis(NASH).In this study,the improvement of SP ethyl acetate extract(SPE)on NASH was evaluated and the potential mechanisms were explored.The glycosides of SPE were determined by HPLC method.Hep G2 cell lines were treated with free fatty acid to clarify the improvement effect and mechanisms of SPE on NASH in vitro.C57BL/6J mice were treated by 60%kcal high-fat diet(HFD)combined with carbon tetrachloride(CCl_4)to establish the NASH model in vivo.The results showed SPE could inhibit the hepatic injury and lipid steatosis by decreasing the levels of alanine aminotransferase,aspartate aminotransferase,triglyceride and total cholesterol in vitro and in vivo.The SPE suppressed the oxidative stress and inflammation by restraining the generation of reactive oxygen species,malondialdehyde,interleukin(IL)-1β,IL-6 and tumor necrosis factor-α(TNF-α)and enhancing the activity of antioxidant enzymes including superoxide dismutase,catalase and glutathione peroxidase in NASH model.Moreover,SPE declined the level of fibrosis markers including hydroxyproline,type I collagen andα-smooth muscle actin(α-SMA)in HFD combined CCl_4-induced mice.Mechanically,SPE inhibited hepatocellular Kelch like ECH associated protein 1 expression and promoted nuclear erythroid 2-related factor 2(Nrf2)nuclear translocation,which suppressed the phosphorylation of IκBα/NF-κB.In addition,SPE down-regulated the level of transforming growth factorβ1(TGF-β1)and phosphorylation of Smad2 to mitigate fibrosis.In brief,SPE could significantly alleviate lipid accumulation,oxidative stress,inflammation and fibrosis via regulating Nrf2/HO-1,IκBα/NF-κB and TGF-β1/Smad2 pathways in the progression of NASH,which indicated that SPE had the potential to be a novel and effective drug or food supplements for the improvement of NASH. 展开更多
关键词 Syringa pubescens Turcz Nonalcoholic steatohepatitis Nuclear erythroid 2-related factor 2 Nuclear factor kappa B Transforming growth factorβ1
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Response of leaf photosynthetic characteristics of Syringa oblata and Syringa reticulata var.mandshurica to chilling stress 认领 引用 被引量：13
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作者 Xiaojia Liu Baiyi An +3 位作者 Na Gu Cainan Guo Xiaogang Sun He Wang 《Journal of Forestry Research》 SCIE CAS CSCD 2020年第2期521-530,共10页
Syringa species not only have good ornamental properties but also play an important role in the landscaping and environmental purification of cities.To investigate the chilling stress resistance of Syringa oblata Lind... Syringa species not only have good ornamental properties but also play an important role in the landscaping and environmental purification of cities.To investigate the chilling stress resistance of Syringa oblata Lindl.and Syringa reticulata var.mandshurica and provide theoretical grounds for the practical cultivation of Syringa species,in vitro leaves were used to study photosynthetic gas exchange parameters and chlorophyll fluorescence parameters.After nine hours of chilling,decreasing rates of net photosynthesis,stomatal conductance,and transpiration in S.reticulata var.mandshurica leaves were significantly greater than that of the S.oblata,while intercellular CO2 concentrations in S.oblata leaves were higher than those in S.reticulata var.mandshurica.The quantum yield of PSII reaction center(APSII)declined in S.reticulata and light capture efficiency(Fv 0/Fm 0)was stable.However,reduction percentages of Fv 0/Fm 0,APSII,and Fv/Fm in S.oblata were significant higher than those of S.reticulata var.mandshurica.After nine hours of chilling,the relative variable fluorescence of VJ and VI of S.oblata increased and the increasing rate of VJ was greater than VI.In contrast,the change of VJ and VI in S.reticulata var.mandshurica leaves was relatively small.This suggests that chilling primarily damaged the electron transport process of QA to QB at the receptor site of the PSII reaction center.Photosynthetic capacity of S.oblata was more sensitive to chilling stress compared to S.reticulate var.mandshurica,which the limitations were mainly due to non-stomatal factors such as the decrease in electron transport efficiency,activity in the PSII reaction center,and the destruction of the photodamage defense system. 展开更多
关键词 Chilling stress Chlorophyll fluorescence characteristics Photosynthetic capacity Syringa oblate Syringa reticulata var.mandshurica
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Two transcription factors,AcREM14 and AcC3H1,enhance the resistance of kiwifruit Actinidia chinensis var.chinensis to Pseudomonas syringae pv.actinidiae 认领 引用 被引量：12
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作者 Chao Zhao Wei Liu +6 位作者 Yali Zhang Yuanzhe Li Chao Ma Runze Tian Rui Li Mingjun Li Lili Huang 《Horticulture Research》 SCIE CSCD 2024年第1期54-69,共16页
Kiwifruit bacterial canker is a global disease caused by Pseudomonas syringae pv.actinidiae(Psa),which poses a major threat to kiwifruit production worldwide.Despite the economic importance of Actinidia chinensis var.... Kiwifruit bacterial canker is a global disease caused by Pseudomonas syringae pv.actinidiae(Psa),which poses a major threat to kiwifruit production worldwide.Despite the economic importance of Actinidia chinensis var.chinensis,only a few resistant varieties have been identified to date.In this study,we screened 44 kiwifruit F1hybrid lines derived from a cross between two A.chinensis var.chinensis lines and identified two offspring with distinct resistance to Psa:resistant offspring RH12 and susceptible offspring SH14.To identify traits associated with resistance,we performed a comparative transcriptomic analysis of these two lines.We identified several highly differentially expressed genes(DEGs)associated with f lavonoid synthesis,pathogen interactions,and hormone signaling pathways,which play essential roles in disease resistance.Additionally,using weighted gene co-expression network analysis,we identified six core transcription factors.Moreover,qRT-PCR results demonstrated the high expression of AcC3H1 and AcREM14 in Psa-induced highly resistant hybrid lines.Ultimately,Overexpression of AcC3H1 and AcREM14 in kiwifruit enhanced disease resistance,and this was associated with upregulation of enzymatic activity and gene expression in the salicylic acid(SA)signaling pathway.Our study elucidates a molecular mechanism underlying disease resistance in kiwifruit and contributes to the advancement of research on kiwifruit breeding. 展开更多
关键词 pseudomonas syringae transcription factors Pseudomonas syringae pv actinidiae kiwifruit bacterial canker Acc h Actinidia chinensis disease resistance Acrem
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引起梨花枯和芽枯的Pseudomonas syringae pv.syringae病原细菌鉴定 认领 引用 被引量：9
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作者 邱文 徐福寿 +5 位作者 谢关林 徐丽慧 怀雁 李斌 余山红 钱军 《中国农业科学》 CAS 北大核心 2008年第9期2657-2662,共6页
【目的】明确在中国发生的梨花枯和芽枯的确切病原菌。【方法】用普通细菌学方法、电镜观察、Koch氏病原假说测定、Biolog、脂肪酸分析、PCR及与标准对照菌株的比较。【结果】从16个病样中分离获得12菌株,6株代表菌株显示出与Pseudomona... 【目的】明确在中国发生的梨花枯和芽枯的确切病原菌。【方法】用普通细菌学方法、电镜观察、Koch氏病原假说测定、Biolog、脂肪酸分析、PCR及与标准对照菌株的比较。【结果】从16个病样中分离获得12菌株,6株代表菌株显示出与Pseudomonas syringaepv.syringae3株标准对照菌株相似的致病反应,它们的Biolog和脂肪酸分析的相似度分别为0.57～0.86和0.58～0.81,PCR和序列测定结合上述结果证实了P.syringaepv.syringae为该病的病原菌。【结论】首次证实了中国梨树上的花枯和芽枯可由P.syringaepv.syringae引起。 展开更多
关键词 梨花枯 梨芽枯 Pseudomonas syringae pv.Syringae 证实
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LaMYC7,a positive regulator of linalool and caryophyllene biosynthesis,confers plant resistance to Pseudomonas syringae 认领 引用 被引量：12
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作者 Yanmei Dong Ziling Wei +5 位作者 Wenying Zhang Jingrui Li Meixian Han Hongtong Bai Hui Li Lei Shi 《Horticulture Research》 SCIE CSCD 2024年第4期78-89,共12页
Linalool and caryophyllene are the main monoterpene and sesquiterpene compounds in lavender;however,the genes regulating their biosynthesis still remain many unknowns.Here,we identified LaMYC7,a positive regulator of ... Linalool and caryophyllene are the main monoterpene and sesquiterpene compounds in lavender;however,the genes regulating their biosynthesis still remain many unknowns.Here,we identified LaMYC7,a positive regulator of linalool and caryophyllene biosynthesis,confers plant resistance to Pseudomonas syringae.LaMYC7 was highly expressed in glandular trichomes,and LaMYC7 overexpression could significantly increase the linalool and caryophyllene contents and reduce susceptibility to P.syringae in Nicotiana.In addition,the linalool possessed antimicrobial activity against P.syringae growth and acted dose-dependently.Further analysis demonstrated that LaMYC7 directly bound to the promoter region of LaTPS76,which encodes the terpene synthase(TPS)for caryophyllene biosynthesis,and that LaTPS76 was highly expressed in glandular trichomes.Notably,the LaMYC7 promoter contained hormone and stress-responsive regulatory elements and responded to various treatments,including ultraviolet,low temperature,salt,drought,methyl jasmonate,and P.syringae infection treatments.Under these treatments,the changes in the linalool and caryophyllene contents were similar to those in LaMYC7 transcript abundance.Based on the results,LaMYC7 could respond to P.syringae infection in addition to being involved in linalool and caryophyllene biosynthesis.Thus,the MYC transcription factor gene LaMYC7 can be used in the breeding of high-yielding linalool and caryophyllene lavender varieties with pathogen resistance. 展开更多
关键词 pseudomonas syringae monoterpene caryophyllene biosynthesis linalool biosynthesis positive regulator pseudomonas syringaelamyc monoterpene sesquiterpene compounds plant resistance
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Insights into the differences in leaf functional traits of heterophyllous Syringa oblata under different light intensities 认领 引用 被引量：16
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作者 Hongguang Xiao Congyan Wang +2 位作者 Jun Liu Lei Wang Daolin Du 《Journal of Forestry Research》 SCIE CAS CSCD 2015年第3期613-621,共9页
Many plants exhibit heterophylly; the spatially and temporally remarkable ontogenetic differences in leaf morphology may play an adaptative role in their success under diverse habitats. Thus, this study aimed to gain ... Many plants exhibit heterophylly; the spatially and temporally remarkable ontogenetic differences in leaf morphology may play an adaptative role in their success under diverse habitats. Thus, this study aimed to gain insights into differences in leaf functional traits of heterophyllous Syringa oblata Lindl., which has been widely used as an ornamental tree around the world under different light intensities in East China. No significant differences existed in specific leaf area （SLA） between lanceolate- and heart-shaped leaves. Differ- ences in the investment per unit of light capture surface area deployed between lanceolate- and heart-shaped leaves may benot obvious. This may be attributing to the fact that single leaf wet and dry weight of heart-shaped leaves were significantly higher than those of lanceolate leaves but leaf length and leaf thickness of heart-shaped leaves were significantly lower than those of lanceolate leaves. The SLA of shade trees was sig- nificantly higher than that of sun trees. The investment per unit of light capture surface of shade trees was lower than that of sun trees, making it possible to increase light capture and use efficiency in low-light environments. The phenotypic plas- ticity of most leaf functional traits of lanceoiate leaves was higher than those of heart-shaped leaves because the former is the juvenile and the latter is the adult leaf shape during the process of phylogenetic development of S. oblate. The higher range of phenotypic plasticity of leaf thickness and leaf moisture for sun trees may be beneficial to obtain a more efficient control of water loss and nutrient deprivation in high- light environments, and the lower range of phenotypic plas- ticity of single leaf wet and dry weight, and SLA for shade trees may gain an advantage to increase resource （especially light） capture and use efficiency in low-light environments. In brief, the successfully ecological strategy of plants is to find an optimal mode for the trade-off between various functional traits to obtain more living resources and achieve more fitness advantage as much as possible in the multivariate environment. 展开更多
关键词 Heterophylly Light intensity Specific leafareas Syringa oblata Lindl.
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植物病原细菌Pseudomonas syringae pv.tomato基因组中的信号肽分析 认领 引用 被引量：12
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作者 刘雅婷 李正跃 +2 位作者 朱有勇 李成云 李永忠 《遗传》 CAS 北大核心 2005年第6期959-964,共6页
应用SignaIP 3.0对植物病原细菌Pseudomonas syringae pv.tomato DC3000菌株基因组中的细菌染色体全部5 615个ORFs进行了分析,确定其中679个ORFs所编码蛋白质的N-端有信号肽序列,其中已经命名并有注释的有107个ORFs.信号肽的长度以19～3... 应用SignaIP 3.0对植物病原细菌Pseudomonas syringae pv.tomato DC3000菌株基因组中的细菌染色体全部5 615个ORFs进行了分析,确定其中679个ORFs所编码蛋白质的N-端有信号肽序列,其中已经命名并有注释的有107个ORFs.信号肽的长度以19～31个氨基酸居多,其中最多的是23个氨基酸的信号肽.具有信号肽的ORFs编码蛋白的长度大多为101～400个氨基酸之间.同时,对组成信号肽的氨基酸种类作了系统的分析,发现组成信号肽的氨基酸中非极性氨基酸占48.54%,极性氨基酸占18.67%,带负电荷氨基酸占24.54%,带正电荷氨基酸仅占8.00%,出现最多的3种氨基酸依次为亮氨酸、丙氨酸和丝氨酸,最少的氨基酸是异亮氨酸,在切割位点-1端的氨基酸中83.21%均为丙氨酸,在切割位点后3位的氨基酸中最多的氨基酸也是丙氨酸.通过分析确定628个分泌类信号肽,36个信号肽具有RR-motif的保守区段,15个脂蛋白类信号肽,未发现Prepiln-like信号肽和Bacteriocin and Pheromone信号肽. 展开更多
关键词 Pseudomonas syringae pv.tomato ORF 信号肽 RR-motif 脂蛋白
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烟草野火病菌Pseudomonas syringae pv.tabaci yuexi-1信号肽预测及分析 认领 引用 被引量：5
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作者 王铁霖 李晶 +1 位作者 杨玉文 赵廷昌 《中国烟草学报》 CAS CSCD 北大核心 2016年第1期92-100,共9页
利用Signal P 4.0、Lipo P 1.0及TMHMM v2.0对烟草野火病菌Pseudomonas syringae pv.tabaci yuexi-1菌株基因组中信号肽的数量、长度和氨基酸组成进行了预测及分类。结果确定其中432个ORFs(Open reading frame)所编码的N端有信号肽序列... 利用Signal P 4.0、Lipo P 1.0及TMHMM v2.0对烟草野火病菌Pseudomonas syringae pv.tabaci yuexi-1菌株基因组中信号肽的数量、长度和氨基酸组成进行了预测及分类。结果确定其中432个ORFs(Open reading frame)所编码的N端有信号肽序列,占全部ORFs的8.81%。其中351条分泌型信号肽(SPI),81条脂蛋白型信号肽(SPII)。在分泌型信号肽中,信号肽的长度为11~42个氨基酸,以长度为22个氨基酸的信号肽最多。同源性分析结果显示,具有相同信号肽序列的不同蛋白序列之间是高度保守的。该研究提供了野火病原菌致病因子的备选基因,提高该病菌致病因子的筛选效率。 展开更多
关键词 Pseudomonas syringae pv.tabaci 信号肽 Signal P 4.0 Lipo P 1.0 TMHMM v2.0
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Vitality and storage condition of Syringa pollen 认领 引用 被引量：8
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作者 周莉 罗凤霞 +1 位作者 代力民 张惠华 《Journal of Forestry Research》 CAS 2003年第1期67-70,共4页
The fresh pollen vitality, the effect of different storage conditions on the pollen vitality, and the difference of vitality among the species of seven species of Syringa were determined in Shenyang, China. The result... The fresh pollen vitality, the effect of different storage conditions on the pollen vitality, and the difference of vitality among the species of seven species of Syringa were determined in Shenyang, China. The results indicated that the pollen vi-tality (81.5%) of Syringa villosa was the highest among the seven tested species, followed by S. microphylla and S. meyeri, and that of S. oblata var. affinis was the lowest. The low temperature was the best condition for storage of pollen of Syringa, and the most proper temperature for the storage was 0-2 癈. The storability of S. microphylla was the best of all, and it could be stored over 60 days at the temperature of 0-2 癈, next was S. villosa and S. meyeri. 展开更多
关键词 Syringa Pollen Vitality Storage condition
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Functional identification of phenazine biosynthesis genes in plant pathogenic bacteria Pseudomonas syringae pv. tomato and Xanthomonas oryzae pv. oryzae 认领 引用 被引量：1
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作者 LI Wen XU You-ping +4 位作者 Jean-Pierre Munyampundu XU Xin QI Xian-fei GU Yuan CAI Xin-zhong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2016年第4期812-821,共10页
Phenazines are secondary metabolites with broad spectrum antibiotic activity and thus show high potential in biological control of pathogens. In this study, we identified phenazine biosynthesis （phz） genes in two ge... Phenazines are secondary metabolites with broad spectrum antibiotic activity and thus show high potential in biological control of pathogens. In this study, we identified phenazine biosynthesis （phz） genes in two genome-completed plant pathogenic bacteria Pseudomonas syringae pv. tomato （Pst） DC3000 and Xanthomonas oryzae pv. oryzae （Xoo） PXO99A. Unlike the phz genes in typical phenazine-producing pseudomonads, phz homologs in Pst DC3000 and Xoo PXO99A consisted of phzC/D/E/F/G and phzC/E1/E2/F/G, respectively, and the both were not organized into an operon. Detection experiments demonstrated that phenazine-l-carboxylic acid （PCA） of Pst DC3000 accumulated to 13.4 IJg L-1, while that of Xoo PXO99A was almost undetectable. Moreover, Pst DC3000 was resistant to 1 mg mL-1 PCA, while Xoo PXO99A was sensitive to 50 IJg mL ~ PCA. Furthermore, mutation of phzF blocked the PCA production and significantly reduced the pathogenicity of Pst DC3000 in tomato, while the complementary strains restored these phenotypes. These results revealed that Pst DC3000 produces low level of and is resistant to phenazines and thus is unable to be biologically controlled by phenazines. Additionally, phz-mediated PCA production is required for full pathogenicity of Pst DC3000. To our knowledge, this is the first report of PCA production and its function in pathogenicity of a plant pathogenic P. syringae strain. 展开更多
关键词 pathogenicity phenazine biosynthesis genes phenazine-l-carboxylic acid plant pathogenic bacteria,Pseudomonas syringae pv. tomato Xanthomonas oryzae pv. oryzae
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Pear Blossom Blast Caused by Pseudomonas syringae pv. syringae in China 认领 引用 被引量：1
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作者 QIU Wen HUAI Yan +5 位作者 XU Fu-shou XU Li-hui XIE Guan-lin LI Bin YU Shan-hong LIU Jun-ying 《Agricultural Sciences in China》 2008年第9期1091-1096,共6页
This study was done to determine the causal organism of the pear blossom and bud blast in China. It was identified by a bacteriological test, electro-microscopic observation, Koch＇s postulate test, Biolog, fatty acid... This study was done to determine the causal organism of the pear blossom and bud blast in China. It was identified by a bacteriological test, electro-microscopic observation, Koch＇s postulate test, Biolog, fatty acid methyl esters （FAMEs）, and a polymerase chain reaction （PCR） test, and compared with the standard reference strains. Six representative strains out of 20 pathogenic bacterial isolates from 16 diseased samples showed characteristics similar to three standard strains of Pseudomonas syringae pv. syringae from Belgium. They were identified as P. syringae pv. syringae with a Biolog similarity of 0.57-0.86 and FAMEs similarity of 0.58-0.81. The bacterium was reisolated from the symptomatic plants and blossoms. Identification as P. syringae pv. syringae was confirmed by using PCR primers and sequence tests, and compared with the above-mentioned results. The data supported the fact that the pear blossom and bud blast in China could be caused by P. syringae pv. syringae. 展开更多
关键词 pear blossom blast pear bud blast Pseudomonas syringae pv. syringae confirmation
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Effects of Different Exogenous Hormones on Rooting of Syringa microphylla Cuttings and Change in Related Enzyme Activity During the Rooting Process 认领 引用 被引量：2
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作者 颜婷美 张安琪 +5 位作者 王峰 李承秀 于永畅 牛田 王郑昊 王长宪 《Agricultural Science & Technology》 CAS 2013年第12期1714-1718,共5页
[Objective] This study aimed to investigate the effects of different exoge- nous hormones on the rooting of Syringa microphylla cuttings and the change in related enzymes activity during the rooting process. [Method] ... [Objective] This study aimed to investigate the effects of different exoge- nous hormones on the rooting of Syringa microphylla cuttings and the change in related enzymes activity during the rooting process. [Method] Three different exoge- nous hormones IBA, NAA and ABT, each with concentrations of 500, 1 000, 1 500 and 2 000 mg/L were used to treat S. microphylla cuttings, and changes in the ac- tivities of peroxidase （POD）, poiyphenol oxidase （PPO） and indoleacetic acid oxidase （IAAO） during the rooting process were also investigated. [Result] The most appro- priate concentrations of IBA, ABT and NAA were 1 500, 1 000 and 1 000 mg/L, respectively, and the 1 500 mg/L IBA treatment exhibited the best effect on rooting. Throughout the rooting process, POD and PPO activities showed the same trends in the treatment groups as those in the control group, but the POD and PPO activi- ties in the treatment groups were increased significantly, with greater amplitude of variation; at the early stage, IAAO activity exhibited an opposite trend between the control group and the treatment groups, which increased slowly in the former, but decreased rapidly in the latter, and it was significantly lower in the treatment groups compared to the control; additionally, higher POD and IAAO activities were con- ducive to the induction of adventitious roots, and lower POD and IAAO activities fa- vored their formation and elongation. [Conclusion] This study has preliminarily clari- fied the rooting mechanism of S. microphylla cuttings. 展开更多
关键词 Syringa microphylla Auxins Cottage rooting Enzyme activity
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Hepatoprotective Effects of Folium syringae Extracts Against Ethanolinduced Acute Liver Injury 认领 引用
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作者 He Jing-shan Lin Yue-xia +7 位作者 Li Chang-wen Li Rui Chang Yi-cong Li Ying Shi Chen-xi Ma Xin Li Zhi Liu Fang-ping 《Journal of Northeast Agricultural University(English Edition)》 CAS 2018年第4期62-70,共9页
The aim of the study was to investigate the hepatoprotective effects of Folium syringae(FS) extracts against ethanolinduced acute liver injury. Mice and primary hepatocytes were pretreated with FS extracts at differen... The aim of the study was to investigate the hepatoprotective effects of Folium syringae(FS) extracts against ethanolinduced acute liver injury. Mice and primary hepatocytes were pretreated with FS extracts at different dosages before ethanol administration. Transaminases, glutathione S-transferase A1 level and hepatic biochemical indices(malondialdehyde, superoxide dismutase, glutathione and glutathione peroxidase) were determined. Pretreatment with FS extracts significantly inhibited the damage caused by ethanol and the hepatoprotective effects of FS were almost similar to Silymarin that was used to treat alcoholic liver injury. GSTA1 contents in all the FS extract-treated groups were significantly different from those in the ethanol-induced acute liver injury model group(p<0.01), and similar trends were observed in transaminases and hepatic indices level both in vitro and in vivo. The results showed that FS extracts had hepatoprotective effects against ethanol-induced injury. Those effects might be related to the enhancement of antioxidant capacity of liver cells, and FS extracts could reduce the release of liver GSTA1, which contributed to improve liver detoxification. 展开更多
关键词 ethanol Folium syringae extract GSTA1 liver injury primary hepatocyte mouse
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Embryo culture and rapid propagation of Syringa 认领 引用
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作者 周莉 代力民 苏宝玲 《Journal of Forestry Research》 CAS 2003年第3期213-216,共4页
Embryo of lilacs (Syringa L) culture in vitro and the rapid propagation were studied. The orthogonal experiments, in-cluding the selection of basal medium, embryo age and other factors such as sugar, benzyladenine (BA... Embryo of lilacs (Syringa L) culture in vitro and the rapid propagation were studied. The orthogonal experiments, in-cluding the selection of basal medium, embryo age and other factors such as sugar, benzyladenine (BA), naphthalene acetic acid (NAA) and glutamine (Gln), were carried out. The results indicated that the optimal medium for embryo culture was Monnier medium supplemented with NAA (0.001 mgL-1), BA (0.1 mgL-1), sugar (50 gL-1), and Gln (400 mgL-1), with a germination rate of 91.7% at least; the optimal embryo age was 50 d; and Gln had significant effects on the germination rate of embryo. Moreover, the optimal medium for subculture was MS+BA (2 mgL-1)+NAA (0.001 mgL-1)+Gln (0.5 mgL-1), with the propaga-tion coefficient of 3.6 at least. 展开更多
关键词 Syringa Embryo-culture Rapid propagation
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Analysis of floral scent emitted from Syringa plants 认领 引用 被引量：9
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作者 Xiaoxia Yang Jing Zhao +5 位作者 Jian Zheng Pingsheng Leng Xiaolong Li Zenghui Hu Jianbin Liu Xin Meng 《Journal of Forestry Research》 SCIE CAS CSCD 2016年第2期273-281,共9页
Syringa is an important aromatic woody angiosperm that is widely planted in gardens. Its dry flowers are traditionally used to manufacture infusions and spices in China. In this study, the floral volatiles emitted fro... Syringa is an important aromatic woody angiosperm that is widely planted in gardens. Its dry flowers are traditionally used to manufacture infusions and spices in China. In this study, the floral volatiles emitted from nine different Syringa species and varieties, viz. S. chinensis, S. protolaciniata, S. oblata, S. oblata var. giraldii, S. oblata var. plena, S. vulgaris ＇Mrs Harry Bickle＇, S. vulgaris ＇Bright Centennial＇, S. vulgaris ＇White Spires＇, and S. vulgaris ＇President Lincoln＇ were collected by the dynamic headspace technique, and then identified by automated thermal desorption-gas chromatography/mass spectrometry. There were significant differences in components and corresponding contents of floral scent emitted from the nine Syringa species and varieties. Among the detected components, β-ocimene exhibited the highest content, accounting for more than 70 % of the floral scent of S. protolaciniata, S. oblata var. giraldii, and S. vulgaris ＇Bright Centennial＇. A significant daytime variation of floral scent emitted from S. pekinensis was recorded, especially for benzaldehyde, whose content first increased and then decreased. We studied the scent emitted from the in vitro flowers of S. vulgaris ＇Pres Lincoln＇ during the process of natural drying. Alcohol compounds were released in the greatest content, and the content of 2-ethyl-l-hexanol accounted for about 40 % of the total release amount. The results described the emission pattern and mechanisms of floral scent in Syringa. 展开更多
关键词 Component Daytime variation Floralscent Relative amount Syringa
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hrpZPsg12 Gene of Pseudomonas syringae pv.glycinea can Enhance Pathogenicity of the Pathogen on Soybean and Cause the Hypersensitive Response of Tobacco 认领 引用
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作者 张佳环 李娟 高洁 《Plant Diseases and Pests》 2011年第3期9-13,共5页
[ Objective ] The paper was to confirrm the effect of hrpZpsg12 gene on the pathogenicity of Pseudomonas syringae pv. glycinea. [ Method ] hrpZpsg12 gene was cloned from P. syringae using PCR method. The knockout plas... [ Objective ] The paper was to confirrm the effect of hrpZpsg12 gene on the pathogenicity of Pseudomonas syringae pv. glycinea. [ Method ] hrpZpsg12 gene was cloned from P. syringae using PCR method. The knockout plasmid pKNOCK-Cm with suicide characteristics and cosmid pUFR034 with complementation func- tion were used to construct the mutation vector pKNOCK477-7 and complementary vector pUFR1026-68 of hrpZpsg12 gene, the mutant 477-1 and the functional com- plementation unit 1026-5 of the gene was also screened out. Three strains including wild-type Psg12, mutant 477-1 and complementary unit 1026-5 were simultane- ously inoculated into soybean leaves and tobacco leaves, then pathogenicity determination and hypersensitive reaction analysis were carried out. [ Result] All the inoculated leaves of soybean and tobacco produced reaction lesion. However, the sizes of reaction lesion were different. The lesion in the leaves inoculated with Psgl2 was relatively large, while the lesion in the leaves inoculated with 477-1 was relatively small; the lesion of complementary unit 1026-5 was similar to wild- type Psgl2. Analysis of reproduction quantity of bacteria in lesions showed that the reproduction quantity of wild-type Psg12 was the highest, while that of mutant 477-1 was the lowest. The reproduction quantity of complementary unit 1026-5 was similar to that of wild-type Psg12. [ Conclusion] hrpZpsg12 gene could enhance the pathogenicity of P. syrimgae on Soybean and produce hypersensitive response in tobacco. 展开更多
关键词 Pseudomonas syringae pv. glycinea hrpZpsg2 gene Mutant Pathogenicity China
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土壤理化性质对暴马丁香(Syringa reticulata var.amurensis)天然林林分因子的影响 认领 引用
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作者 姚永刚 蒲凌奎 +2 位作者 朱媛君 鄢鹏 王前 《林业科技通讯》 2018年第7期3-7,共5页
通过对处于不同坡位的暴马丁香（Syringa reticulata var.amurensis）天然林的土壤理化性质及林分因子的研究,探讨了暴马丁香天然林的林分特征及不同土壤理化性质对林分因子的影响。认为：在不同坡位样地中,处于坡中位置的暴马丁香林分... 通过对处于不同坡位的暴马丁香（Syringa reticulata var.amurensis）天然林的土壤理化性质及林分因子的研究,探讨了暴马丁香天然林的林分特征及不同土壤理化性质对林分因子的影响。认为：在不同坡位样地中,处于坡中位置的暴马丁香林分因子占优势地位;土壤全磷含量、全钾含量、速效钾含量及有效磷含量对暴马丁香各林分因子有显著相关性。 展开更多
关键词 暴马丁香 Syringa reticulata var.amurensis 土壤理化性质 林分因子 天然林
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