Toona fargesi A.Chev.,a versatile tree in the Toona genus of the Meliaceae family,is renowned for its exquisite timber and medicinal properties,offering promising benefits.Due to natural regeneration obstacles and lon...Toona fargesi A.Chev.,a versatile tree in the Toona genus of the Meliaceae family,is renowned for its exquisite timber and medicinal properties,offering promising benefits.Due to natural regeneration obstacles and long-term excessive exploitation,it has been threatened in China.Intriguingly,root sprouting,which may diminish the genetic diversity and hinder population development,dominates the reproductive pattern of T.fargesi in the wild.However,the lack of complete genome information has hampered basic studies on the regeneration,classification,evolution and conservation of this species.Here,we report the genome of T.fargesi,which was sequenced using the PacBio platform and assembled into a high-quality genome with a total size of 535.24 Mb.Of this,97.93%of the assembled contigs were anchored onto 28 pseudochromosomes,achieving a chromosome-level genome.The long terminal repeat assembly index score was 21.34,and the consensus quality value was 39.90%,indicating the accuracy and completeness of the genome.Comparative genome analysis suggested that a recent whole genome duplication event occurred between 22.1 and 5o.1 Mya in the Toona genus,with the divergence time between T.fargesi and its relative T.sinensis estimated at approximately~16.7 Mya.Additionally,13 TfARR genes,which play integral roles in root sprouting by mediating cytokinin signaling,underwent rapid gene expansion and showed significant enrichment in the plant hormone signal transduction pathway.Furthermore,transcriptomic analysis demonstrated that differentially expressed genes between root sprouts and nonroot sprouts were significantly enriched in the zeatin biosynthesis pathway,indicating that cytokinin regulation is involved in root sprouting development.Collectively,the findings provide valuable genomic resources for the Toona genus and genetic insights into the mechanisms of root sprouting in T.fargesii.展开更多
Unmanaged wood waste,particularly in countries like Nepal,presents serious environmental concerns due to open burning and improper disposal,leading to carbon emissions,air pollution and land degradation.This study int...Unmanaged wood waste,particularly in countries like Nepal,presents serious environmental concerns due to open burning and improper disposal,leading to carbon emissions,air pollution and land degradation.This study introduces an environmentally sustainable strategy to upcycle Toona ciliata wood scrap—an abundant and underutilized lignocellulosic biomass—into high performance carbon electrodes for green energy storage applications.Activated carbon(TCWAC)was synthesized via single-step pyrolytic carbonization followed by phosphoric acid activation,yielding a material with high specific surface area,hierarchical porosity,and excellent electrical conductivity.Electrochemical measurements using a three-electrode configuration in 6 M KOH revealed optimized potential windows of -1.0 to -0.2 V(TCWAC),-1.2 to 0 V(TCWAC-Mn),and -1.15 to -0.4 V(TCWAC-Fe).TCWAC exhibited a specific capacitance of 156.3 Fg-1at 1 Ag-1,with an energy density of 3.5 Whkg-1,and 80.2% capacity retention after 1000 charge-discharge cycles.Composites with MnO2and Fe2O3were also evaluated.TWAC-Mn delivered 489.4 Fg-1,25.1 Whkg-1,and 99.1% retention,whereas,TWAC-Fe achieved 321.3 Fg-1,6.3 Whkg-1,and 90.3% retention.The superior performance of MnO2is attributed to its multiple oxidation states,facilitating reversible faradaic redox and enhanced pseudocapacitance.This work offers the first direct,systematic comparison of MnO2and Fe2O3composites on a common biomass-carbon matrix under identical synthesis and testing conditions.The finding provides mechanistic insight into charge storage behaviour and demonstrate a scalable route for converting biomass waste into sustainable electrode materials,contributing to cleaner energy solutions and improved biomass valorization.展开更多
Toona ciliata and Toona ciliata var. pubescens are timber species of national second-class protective plants. In China, Toona ciliata and Toona ciliata var.pubescens distribute sporadically with small population size ...Toona ciliata and Toona ciliata var. pubescens are timber species of national second-class protective plants. In China, Toona ciliata and Toona ciliata var.pubescens distribute sporadically with small population size but with great development potential. Plus selection on phenotype of Toona ciliata and Toona ciliata var.pubescens, molecular genetic marker of Toona ciliata var. pubescens, research progress in physiology and ecology of Toona ciliata and Toona ciliata var.pubescens were summarized in this paper. Suggestions and prospect forecast were proposed for relative research and utilization.展开更多
Toona ciliata and Toona ciliata var. pubescens are rare timber species. In China, Toona ciliata and Toona ciliata var. pubescens distribute sporadical y with smal popuIation size but have huge deveIopment potential. T...Toona ciliata and Toona ciliata var. pubescens are rare timber species. In China, Toona ciliata and Toona ciliata var. pubescens distribute sporadical y with smal popuIation size but have huge deveIopment potential. The paper reviewed the research progress of their seedIing breeding, nutrient characteristics, fertiIization technoIogy, afforestation design, timber utiIization, chemistry and pharmacoIogy, and then Iooked into the future research and utiIization of Toona ciliata and Toona ciliata var. pubescens.展开更多
The study of plant species abundance distribution(SAD)in natural communities is of considerable importance to understand the processes and ecological rules of community assembly.With the distribution of tree,shrub and...The study of plant species abundance distribution(SAD)in natural communities is of considerable importance to understand the processes and ecological rules of community assembly.With the distribution of tree,shrub and herb layers of eight natural communities of Toona ciliata as research targets,three diff erent ecological niche models were used:broken stick model,overlapping niche model and niche preemption model,as well as three statistical models:log-series distribution model,log-normal distribution model and Weibull distribution model,to fi t SAD of the diff erent vegetation layers based on data collected.Goodness-of-fi t was compared with Chi square test,Kolmogorov–Smirnov(K–S)test and Akaike Information Criterion(AIC).The results show:(1)based on the criteria of the lowest AIC value,Chi square value and K–S value with no signifi cant diff erence(p>0.05)between theoretic and observed SADs.The suitability and goodness-of-fi t of the broken stick model was the best of three ecological niche models.The log-series distribution model did not accept the fi tted results of most vegetation layers and had the lowest goodness-of-fi t.The Weibull distribution model had the best goodness-of-fi t for SADs.Overall,the statistical SADs performed better than the ecological ones.(2)T.ciliata was the dominant species in all the communities;species richness and diversity of herbs were the highest of the vegetation layers,while the diversities of the tree layers were slightly higher than the shrub layers;there were fewer common species and more rare species in the eight communities.The herb layers had the highest community evenness,followed by the shrub and the tree layers.Due to the complexity and habitat diversity of the diff erent T.ciliata communities,comprehensive analyses of a variety of SADs and tests for optimal models together with management,are practical steps to enhance understanding of ecological processes and mechanisms of T.ciliata communities,to detect disturbances,and to facilitate biodiversity and species conservation.展开更多
Chinese mahogany(Toona sinensis) is of considerable medical and economic importance, and its genome has been deciphered. However, the process underlying its polyploidy is unclear, and the chromosomal evolutionary traj...Chinese mahogany(Toona sinensis) is of considerable medical and economic importance, and its genome has been deciphered. However, the process underlying its polyploidy is unclear, and the chromosomal evolutionary trajectory is poorly understood. Here, by reanalysing the T.sinensis genome, we found evidence of a tetraploidization event(T. sinensis special tetraploidization, TST) that occurred approximately 15-17 million years ago(MYA) after the core eudicot-common hexaploidization(ECH or gamma) event. We characterized the synonymous nucleotide substitution rates(Ks values) of collinear genes and found that T. sinensis genes affected by the TST evolve at a slower rate than Acer yangbiense genes. Furthermore, we identified homologous genes related to polyploidization and speciation and constructed multiple alignments with different reference genomes. Notably, the significant balance of gene retention and loss characterized in the two TST-derived subgenomes suggests an autopolyploid nature of the TST. Moreover, we deduced the chromosomal karyotypes of the two subgenomes and identified 7chromosomal fusions that have shaped the T. sinensis genome;more information is available on a newly constructed karyotype platform(http://gffzze84c5b42976d4cd7hkkn60fo5ov9066no.ffgz.tsg.suse.edu.cn/Toona_sinensis/index.html). The T. sinensis genome preserves the ancestral chromosome structure of dicotyledons well and could serve as a good reference for understanding genomic changes in other Meliaceae and Sapindales plants. In addition, we verified that tandem duplication and the ECH have promoted the expansion of terpene synthase(TPS) genes;conversely, the TST seems to have inhibited expansion of these genes. This present effort has clarified the polyploidy events of the T. sinensis genome, filled gaps in the history of karyotype evolution, and laid a solid foundation for further genomic studies in the Meliaceae research community and beyond.展开更多
ln order to discuss the optimum seedling density of Toona ciliate, different seedling densities were studied. The results showed that seeding height and basal diameter decreased and quality of the seedlings declined w...ln order to discuss the optimum seedling density of Toona ciliate, different seedling densities were studied. The results showed that seeding height and basal diameter decreased and quality of the seedlings declined with the increase of seedling density. The reasonable density of seedlings is key to cultivate high-quality seedlings and achieve the best seedling efficiency. The optimum density was 30 plants/m2 for the first class seedlings of biennial seedlings, and the optimum density of l and ll seedlings for production afforestation was 40 plants/m2.展开更多
Cutting propagation rhizogenesis of Toona ciliata clone cutting orchard were studied, and the results were as fol ows: Rooting type included both cal us rooting and cortex rooting. About 17 in 22 of clones were domin...Cutting propagation rhizogenesis of Toona ciliata clone cutting orchard were studied, and the results were as fol ows: Rooting type included both cal us rooting and cortex rooting. About 17 in 22 of clones were dominated by cortex rooting, accounting for the 77% of clones. There were 5 clones which occurred with both cal us rooting and cortex rooting during rooting process. The rooting efficiency index (REI) showed a positive correlation with the rooting rate. Under natural tem-perature condition, keeping moisture and shading, we found that the highest survival rate occurred in early June, September, and October.展开更多
In this study, a screening experiment was carried out among MS, WPM, DCR, MT, B5 and N6. The results showed that for proliferation culture of Toona ciliate, the optimum basic medium was WPM, and the optimum medium was...In this study, a screening experiment was carried out among MS, WPM, DCR, MT, B5 and N6. The results showed that for proliferation culture of Toona ciliate, the optimum basic medium was WPM, and the optimum medium was WPM + 6-BA 0.5 mg/L + IBA 0.05 mg/L. The proliferation coefficient reached 4.29. The optimum rooting culture medium was 1/2 MS + IBA 0.5 mg/L + NAA 0.5 mg/L with rooting rate of 100%. In the optimum rooting 'culture medium, the roots of T. ciliate developed neatly, and the seedlings grew well.展开更多
Using seed sowing cultivation method, the four-season soilless cultivation of the sprouting seedlings of Toona sinensis was carried out with different germination accelerating treatments, cultivation substrates, culti...Using seed sowing cultivation method, the four-season soilless cultivation of the sprouting seedlings of Toona sinensis was carried out with different germination accelerating treatments, cultivation substrates, cultivation environment and management technologies by using the corresponding treatment methods in different seasons, so as to produce the sprouting vegetable products of T. sinensis which was not restricted by seasons. The test results showed that using the mixed substrate of pedite and vermiculite in winter and spring can keep moisture but not too high, and the produced sprout vegetable had good quality and high yield.展开更多
In order to collect rainwater and resist drought to enhance the utilization rate of rainfall and water resources, through project rainwater harvesting measures, the total annual rainwater harvesting amount of the six ...In order to collect rainwater and resist drought to enhance the utilization rate of rainfall and water resources, through project rainwater harvesting measures, the total annual rainwater harvesting amount of the six greenhouses was calculated according to annual average precipitation 542.2 mm, up to 1 095.7 m^3. The upper natural slopes of cultivated land were as rainwater harvesting areas, and total annual rainwater harvesting amount was 49 242 m^3 on the mountain slopes with an area of 73.37 hm^2, while total water storage amount was 39 394 m^3 in theory, so it could meet water use for the irrigation of 26.28 hm^2 of T. sinensis land. To be convenient for rainwater harvesting, irrigation and supplying water to the water-saving cellars, one pert-cut and part-fill reservoir (which was 470 m^3 in volume) was built on the mountain slopes at the right rear of the greenhouses, and their altitude difference was 50 m. The reservoir was sealed and was built with reinforced concrete. Water-saving cellars were distributed in front and the middle and at the back of two rows of greenhouses, and they were connected with each other. The reservoir could supply water to the water-saving cellars and also collect water by mountain slopes, from the lower water-saving cellars or deep wells. Two rainwater hervesting ditches that were 1 650 m in length were at the lower edge of arable land in the upper reaches of slopes to intercept rainfall runoff and make it flow into channels and then the sedimentation tanks. The total annual rainwater harvesting amount of the reservoir and water-saving cellars was 1 222.5 m^3.展开更多
Toona ciliate,which belongs to Toona of Meliaceae,is a rare and native timber species in China.However,little progress has been made in the cultivation of Toona ciliate.The height and diameter at breast height of Toon...Toona ciliate,which belongs to Toona of Meliaceae,is a rare and native timber species in China.However,little progress has been made in the cultivation of Toona ciliate.The height and diameter at breast height of Toona ciliata were assessed for fertilization cultivation techniques aged 1 year.Analysis of variance showed that there were significant differences on height among different treatments and replicates and the difference on diameter at breast height among different replicate was also significantly.Treatment with 200 g urea(N>46.5%)+60 g superphosphate(P2O5>12.0%)+100 g potassium chloride(K2O>60.0%)could improve the height and diameter at breast height of Toona ciliate.In summary,more fertilizer could not always improve the growth traits and the site location could affect the growth significantly,therefore more test trials should be carried out before diffusion.展开更多
We used the cotyledons and cotyledonary nodes of Toona ciliata(Chinese mahogany)as explants to examine callus and adventitious shoot induction when exposed to different ratios of hormones.We also investigated the effe...We used the cotyledons and cotyledonary nodes of Toona ciliata(Chinese mahogany)as explants to examine callus and adventitious shoot induction when exposed to different ratios of hormones.We also investigated the effects of seedling age,inoculation method,and genotype on the efficient regeneration of T.ciliata.The results showed that different genotypes exhibited significantly different callus induction efficiency.The cotyledons and cotyledonary nodes of 20-day seedlings inoculated onto MS medium with 0.5 mg/L 6-benzylaminopurine(6-BA),0.5 mg/L kinetin(KT)and 0.05 mg/L 1-naphthylacetic acid(NAA)achieved a greater regeneration rate than did other concentrations of cytokinin and auxin.The numbers of shoots per cotyledon and cotyledonary node explant were 7.33 and 6.67.The optimal inoculation method for cotyledons was that the distal end of the explants was placed in contact with the medium.The optimal adventitious shoot differentiation medium for cotyledon explants was MS medium containing 0.3 mg/L 6-BA and 0.2 mg/L NAA,producing a 3.4 cm height of shoot on average.This study established an efficient regeneration system for T.ciliata with cotyledons and cotyledonary nodes as explants.展开更多
Three new limonoid-type triterpenoids,namely toonasins A–C(1–3)with a rare lactam E ring,along with six known compounds(4–9)were isolated from the barks of Toona sinensis.The structures of new compounds were elucid...Three new limonoid-type triterpenoids,namely toonasins A–C(1–3)with a rare lactam E ring,along with six known compounds(4–9)were isolated from the barks of Toona sinensis.The structures of new compounds were elucidated by interpretation of spectroscopic data,and the relative configuration of compound 1 was further characterized by X-ray crystallographic analyses.The isolated compounds were evaluated for their cytotoxic activities against five human tumor cell lines(HL-60,SMMC-7721,A-549,MCF-7 and SW480),and compounds 3 and 5 showed weak cytotoxicities.展开更多
[ Objective ] The repellent activities and contact toxicity of extracts from Toona sinensis against Sitophilus zeamais were studied. [ Meth- od] 95% alcohol, petroleum ether and dichloromethane were selected to extrac...[ Objective ] The repellent activities and contact toxicity of extracts from Toona sinensis against Sitophilus zeamais were studied. [ Meth- od] 95% alcohol, petroleum ether and dichloromethane were selected to extract the active components from the leaves and barks of T. sinensis, respectively. And then the extracts were prepared at 0.6, 0.3, 0.15 g/ml to study the biological activity against S. zeamais in lab. [ Result J The petroleum ether extracts from the leaves of T. sinensis had significantly repellent effects on S. zeamais, but the extracts from T. sinensis with three solvents had no obvious contact toxicity against S. zeamais. [ Conclusion ] The petroleum ether extracts from S. zeamais had certain repellent activity.展开更多
基金supported by grants from the National Natural Science Foundation of China(32460387,32101515,and 31860202)Jiangxi Provincial Department of Education in ScienceandTechnology Project(GJJ200447).
摘要Toona fargesi A.Chev.,a versatile tree in the Toona genus of the Meliaceae family,is renowned for its exquisite timber and medicinal properties,offering promising benefits.Due to natural regeneration obstacles and long-term excessive exploitation,it has been threatened in China.Intriguingly,root sprouting,which may diminish the genetic diversity and hinder population development,dominates the reproductive pattern of T.fargesi in the wild.However,the lack of complete genome information has hampered basic studies on the regeneration,classification,evolution and conservation of this species.Here,we report the genome of T.fargesi,which was sequenced using the PacBio platform and assembled into a high-quality genome with a total size of 535.24 Mb.Of this,97.93%of the assembled contigs were anchored onto 28 pseudochromosomes,achieving a chromosome-level genome.The long terminal repeat assembly index score was 21.34,and the consensus quality value was 39.90%,indicating the accuracy and completeness of the genome.Comparative genome analysis suggested that a recent whole genome duplication event occurred between 22.1 and 5o.1 Mya in the Toona genus,with the divergence time between T.fargesi and its relative T.sinensis estimated at approximately~16.7 Mya.Additionally,13 TfARR genes,which play integral roles in root sprouting by mediating cytokinin signaling,underwent rapid gene expansion and showed significant enrichment in the plant hormone signal transduction pathway.Furthermore,transcriptomic analysis demonstrated that differentially expressed genes between root sprouts and nonroot sprouts were significantly enriched in the zeatin biosynthesis pathway,indicating that cytokinin regulation is involved in root sprouting development.Collectively,the findings provide valuable genomic resources for the Toona genus and genetic insights into the mechanisms of root sprouting in T.fargesii.
摘要Unmanaged wood waste,particularly in countries like Nepal,presents serious environmental concerns due to open burning and improper disposal,leading to carbon emissions,air pollution and land degradation.This study introduces an environmentally sustainable strategy to upcycle Toona ciliata wood scrap—an abundant and underutilized lignocellulosic biomass—into high performance carbon electrodes for green energy storage applications.Activated carbon(TCWAC)was synthesized via single-step pyrolytic carbonization followed by phosphoric acid activation,yielding a material with high specific surface area,hierarchical porosity,and excellent electrical conductivity.Electrochemical measurements using a three-electrode configuration in 6 M KOH revealed optimized potential windows of -1.0 to -0.2 V(TCWAC),-1.2 to 0 V(TCWAC-Mn),and -1.15 to -0.4 V(TCWAC-Fe).TCWAC exhibited a specific capacitance of 156.3 Fg-1at 1 Ag-1,with an energy density of 3.5 Whkg-1,and 80.2% capacity retention after 1000 charge-discharge cycles.Composites with MnO2and Fe2O3were also evaluated.TWAC-Mn delivered 489.4 Fg-1,25.1 Whkg-1,and 99.1% retention,whereas,TWAC-Fe achieved 321.3 Fg-1,6.3 Whkg-1,and 90.3% retention.The superior performance of MnO2is attributed to its multiple oxidation states,facilitating reversible faradaic redox and enhanced pseudocapacitance.This work offers the first direct,systematic comparison of MnO2and Fe2O3composites on a common biomass-carbon matrix under identical synthesis and testing conditions.The finding provides mechanistic insight into charge storage behaviour and demonstrate a scalable route for converting biomass waste into sustainable electrode materials,contributing to cleaner energy solutions and improved biomass valorization.
基金Supported by Public Welfare Research Projects of Science and Technology Bureau of Hubei Province(40 2012DBA40001)~~
摘要Toona ciliata and Toona ciliata var. pubescens are timber species of national second-class protective plants. In China, Toona ciliata and Toona ciliata var.pubescens distribute sporadically with small population size but with great development potential. Plus selection on phenotype of Toona ciliata and Toona ciliata var.pubescens, molecular genetic marker of Toona ciliata var. pubescens, research progress in physiology and ecology of Toona ciliata and Toona ciliata var.pubescens were summarized in this paper. Suggestions and prospect forecast were proposed for relative research and utilization.
基金Supported by Public Welfare Research Projects of Science and Technology Bureau of Hubei Province(40 2012DBA40001)~~
摘要Toona ciliata and Toona ciliata var. pubescens are rare timber species. In China, Toona ciliata and Toona ciliata var. pubescens distribute sporadical y with smal popuIation size but have huge deveIopment potential. The paper reviewed the research progress of their seedIing breeding, nutrient characteristics, fertiIization technoIogy, afforestation design, timber utiIization, chemistry and pharmacoIogy, and then Iooked into the future research and utiIization of Toona ciliata and Toona ciliata var. pubescens.
基金Hubei Provincial Department of Science and Technology,under the public welfare research project[No.402012DBA40001]Hubei Provincial Department of Education,under the scientifi c research project[No.B20160555].
摘要The study of plant species abundance distribution(SAD)in natural communities is of considerable importance to understand the processes and ecological rules of community assembly.With the distribution of tree,shrub and herb layers of eight natural communities of Toona ciliata as research targets,three diff erent ecological niche models were used:broken stick model,overlapping niche model and niche preemption model,as well as three statistical models:log-series distribution model,log-normal distribution model and Weibull distribution model,to fi t SAD of the diff erent vegetation layers based on data collected.Goodness-of-fi t was compared with Chi square test,Kolmogorov–Smirnov(K–S)test and Akaike Information Criterion(AIC).The results show:(1)based on the criteria of the lowest AIC value,Chi square value and K–S value with no signifi cant diff erence(p>0.05)between theoretic and observed SADs.The suitability and goodness-of-fi t of the broken stick model was the best of three ecological niche models.The log-series distribution model did not accept the fi tted results of most vegetation layers and had the lowest goodness-of-fi t.The Weibull distribution model had the best goodness-of-fi t for SADs.Overall,the statistical SADs performed better than the ecological ones.(2)T.ciliata was the dominant species in all the communities;species richness and diversity of herbs were the highest of the vegetation layers,while the diversities of the tree layers were slightly higher than the shrub layers;there were fewer common species and more rare species in the eight communities.The herb layers had the highest community evenness,followed by the shrub and the tree layers.Due to the complexity and habitat diversity of the diff erent T.ciliata communities,comprehensive analyses of a variety of SADs and tests for optimal models together with management,are practical steps to enhance understanding of ecological processes and mechanisms of T.ciliata communities,to detect disturbances,and to facilitate biodiversity and species conservation.
基金supported by the National Natural Science Foundation of China(Grant No.32170236 and 31501333)the Natural Science Foundation of Hebei Province(Grant No.C2020209064)+2 种基金the Project of Youth Fund for National Natural Science Foundation of China(Grant No.32001791)the Tangshan Science and Technology Planning Project(Grant No.20150209C)the innovation and entrepreneurship training program for college students of North China University of Science and Technology(Grant No.X2019256).
摘要Chinese mahogany(Toona sinensis) is of considerable medical and economic importance, and its genome has been deciphered. However, the process underlying its polyploidy is unclear, and the chromosomal evolutionary trajectory is poorly understood. Here, by reanalysing the T.sinensis genome, we found evidence of a tetraploidization event(T. sinensis special tetraploidization, TST) that occurred approximately 15-17 million years ago(MYA) after the core eudicot-common hexaploidization(ECH or gamma) event. We characterized the synonymous nucleotide substitution rates(Ks values) of collinear genes and found that T. sinensis genes affected by the TST evolve at a slower rate than Acer yangbiense genes. Furthermore, we identified homologous genes related to polyploidization and speciation and constructed multiple alignments with different reference genomes. Notably, the significant balance of gene retention and loss characterized in the two TST-derived subgenomes suggests an autopolyploid nature of the TST. Moreover, we deduced the chromosomal karyotypes of the two subgenomes and identified 7chromosomal fusions that have shaped the T. sinensis genome;more information is available on a newly constructed karyotype platform(http://gffzze84c5b42976d4cd7hkkn60fo5ov9066no.ffgz.tsg.suse.edu.cn/Toona_sinensis/index.html). The T. sinensis genome preserves the ancestral chromosome structure of dicotyledons well and could serve as a good reference for understanding genomic changes in other Meliaceae and Sapindales plants. In addition, we verified that tandem duplication and the ECH have promoted the expansion of terpene synthase(TPS) genes;conversely, the TST seems to have inhibited expansion of these genes. This present effort has clarified the polyploidy events of the T. sinensis genome, filled gaps in the history of karyotype evolution, and laid a solid foundation for further genomic studies in the Meliaceae research community and beyond.
基金Supported by Forestry Science and Technology Planning Program(XLK201406)~~
摘要ln order to discuss the optimum seedling density of Toona ciliate, different seedling densities were studied. The results showed that seeding height and basal diameter decreased and quality of the seedlings declined with the increase of seedling density. The reasonable density of seedlings is key to cultivate high-quality seedlings and achieve the best seedling efficiency. The optimum density was 30 plants/m2 for the first class seedlings of biennial seedlings, and the optimum density of l and ll seedlings for production afforestation was 40 plants/m2.
基金Supported by Hunan Forestry Science and Technology Project(XLK201406)~~
摘要Cutting propagation rhizogenesis of Toona ciliata clone cutting orchard were studied, and the results were as fol ows: Rooting type included both cal us rooting and cortex rooting. About 17 in 22 of clones were dominated by cortex rooting, accounting for the 77% of clones. There were 5 clones which occurred with both cal us rooting and cortex rooting during rooting process. The rooting efficiency index (REI) showed a positive correlation with the rooting rate. Under natural tem-perature condition, keeping moisture and shading, we found that the highest survival rate occurred in early June, September, and October.
摘要In this study, a screening experiment was carried out among MS, WPM, DCR, MT, B5 and N6. The results showed that for proliferation culture of Toona ciliate, the optimum basic medium was WPM, and the optimum medium was WPM + 6-BA 0.5 mg/L + IBA 0.05 mg/L. The proliferation coefficient reached 4.29. The optimum rooting culture medium was 1/2 MS + IBA 0.5 mg/L + NAA 0.5 mg/L with rooting rate of 100%. In the optimum rooting 'culture medium, the roots of T. ciliate developed neatly, and the seedlings grew well.
摘要Using seed sowing cultivation method, the four-season soilless cultivation of the sprouting seedlings of Toona sinensis was carried out with different germination accelerating treatments, cultivation substrates, cultivation environment and management technologies by using the corresponding treatment methods in different seasons, so as to produce the sprouting vegetable products of T. sinensis which was not restricted by seasons. The test results showed that using the mixed substrate of pedite and vermiculite in winter and spring can keep moisture but not too high, and the produced sprout vegetable had good quality and high yield.
基金Supported by Key Technology R&D Program Project of Shijiazhuang City(141520208A)~~
摘要In order to collect rainwater and resist drought to enhance the utilization rate of rainfall and water resources, through project rainwater harvesting measures, the total annual rainwater harvesting amount of the six greenhouses was calculated according to annual average precipitation 542.2 mm, up to 1 095.7 m^3. The upper natural slopes of cultivated land were as rainwater harvesting areas, and total annual rainwater harvesting amount was 49 242 m^3 on the mountain slopes with an area of 73.37 hm^2, while total water storage amount was 39 394 m^3 in theory, so it could meet water use for the irrigation of 26.28 hm^2 of T. sinensis land. To be convenient for rainwater harvesting, irrigation and supplying water to the water-saving cellars, one pert-cut and part-fill reservoir (which was 470 m^3 in volume) was built on the mountain slopes at the right rear of the greenhouses, and their altitude difference was 50 m. The reservoir was sealed and was built with reinforced concrete. Water-saving cellars were distributed in front and the middle and at the back of two rows of greenhouses, and they were connected with each other. The reservoir could supply water to the water-saving cellars and also collect water by mountain slopes, from the lower water-saving cellars or deep wells. Two rainwater hervesting ditches that were 1 650 m in length were at the lower edge of arable land in the upper reaches of slopes to intercept rainfall runoff and make it flow into channels and then the sedimentation tanks. The total annual rainwater harvesting amount of the reservoir and water-saving cellars was 1 222.5 m^3.
摘要Toona ciliate,which belongs to Toona of Meliaceae,is a rare and native timber species in China.However,little progress has been made in the cultivation of Toona ciliate.The height and diameter at breast height of Toona ciliata were assessed for fertilization cultivation techniques aged 1 year.Analysis of variance showed that there were significant differences on height among different treatments and replicates and the difference on diameter at breast height among different replicate was also significantly.Treatment with 200 g urea(N>46.5%)+60 g superphosphate(P2O5>12.0%)+100 g potassium chloride(K2O>60.0%)could improve the height and diameter at breast height of Toona ciliate.In summary,more fertilizer could not always improve the growth traits and the site location could affect the growth significantly,therefore more test trials should be carried out before diffusion.
基金This work was fi nancially supported by the National Key Research Projects,Forestry Resource Cultivation and Utilization Technology Innovation(Grant No.2016YFD0600606)the Natural Science Foundation of Guangdong Province of China(Grant No.2018A030313798)Characteristic innovation projects of department of education of Guangdong province(Grant No.2019KTSCX017).
摘要We used the cotyledons and cotyledonary nodes of Toona ciliata(Chinese mahogany)as explants to examine callus and adventitious shoot induction when exposed to different ratios of hormones.We also investigated the effects of seedling age,inoculation method,and genotype on the efficient regeneration of T.ciliata.The results showed that different genotypes exhibited significantly different callus induction efficiency.The cotyledons and cotyledonary nodes of 20-day seedlings inoculated onto MS medium with 0.5 mg/L 6-benzylaminopurine(6-BA),0.5 mg/L kinetin(KT)and 0.05 mg/L 1-naphthylacetic acid(NAA)achieved a greater regeneration rate than did other concentrations of cytokinin and auxin.The numbers of shoots per cotyledon and cotyledonary node explant were 7.33 and 6.67.The optimal inoculation method for cotyledons was that the distal end of the explants was placed in contact with the medium.The optimal adventitious shoot differentiation medium for cotyledon explants was MS medium containing 0.3 mg/L 6-BA and 0.2 mg/L NAA,producing a 3.4 cm height of shoot on average.This study established an efficient regeneration system for T.ciliata with cotyledons and cotyledonary nodes as explants.
基金the National Knowledge Innovation of CAS(No.KSCX2-YW-G-038)the Foundation of State Key Laboratory of Phytochemistry and Plant Resources in West China(P2015-ZZ09)。
摘要Three new limonoid-type triterpenoids,namely toonasins A–C(1–3)with a rare lactam E ring,along with six known compounds(4–9)were isolated from the barks of Toona sinensis.The structures of new compounds were elucidated by interpretation of spectroscopic data,and the relative configuration of compound 1 was further characterized by X-ray crystallographic analyses.The isolated compounds were evaluated for their cytotoxic activities against five human tumor cell lines(HL-60,SMMC-7721,A-549,MCF-7 and SW480),and compounds 3 and 5 showed weak cytotoxicities.
基金Supported by Scientific Research Foundation of China West Normal University(07B006)~~
摘要[ Objective ] The repellent activities and contact toxicity of extracts from Toona sinensis against Sitophilus zeamais were studied. [ Meth- od] 95% alcohol, petroleum ether and dichloromethane were selected to extract the active components from the leaves and barks of T. sinensis, respectively. And then the extracts were prepared at 0.6, 0.3, 0.15 g/ml to study the biological activity against S. zeamais in lab. [ Result J The petroleum ether extracts from the leaves of T. sinensis had significantly repellent effects on S. zeamais, but the extracts from T. sinensis with three solvents had no obvious contact toxicity against S. zeamais. [ Conclusion ] The petroleum ether extracts from S. zeamais had certain repellent activity.