期刊文献+
任意字段
题名或关键词
题名
关键词
摘要
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息
共找到329篇文章
< 1 2 … 17 >
每页显示 20 50 100
已选0条
引用分析
参考文献
引证文献
 统计分析
检索结果
已选文献
显示方式：
Construction of single chain Fv antibody against transferrin receptor and its protein fusion with alkaline phosphatase 认领 引用 被引量：13
1
作者 Dao-FengYang Hui-FenZhu +2 位作者 Zhi-HuaWang Guan-XinShen De-YingTian 《World Journal of Gastroenterology》 SCIE CAS 2005年第21期3300-3303,共4页
AIM: To construct fusion protein of a single-chain antibody (scFv) against transferrin receptor (TfR) with alkaline phosphatase(AP). METHODS: The VH-linker-VL,namely scFv gene,was prepared by amplifying the VH and VL ... AIM: To construct fusion protein of a single-chain antibody (scFv) against transferrin receptor (TfR) with alkaline phosphatase(AP). METHODS: The VH-linker-VL,namely scFv gene,was prepared by amplifying the VH and VL genes from plasmid pGEM-T-VH and pGEM-T-VL with splicing overlap extension polymerase chain reaction (SOE PCR). After the ScFv gene was modified by 5/71 and Not I,it was subcloned into the secretory expression vector pUC19/119, and then was transformed into E.coli TG1.The positive colonies were screened by colony PCR and their expressions were induced by IPTG.ScFv gene was gained by digesting ScFv expression vector pUC19/119 with 5/71 and NotI restriction enzymes, then subcloned into expression vector pDAP2, followed by transformation in E.coli TG1.The positive colonies were selected by bacterial colony PCR.The expression of fusion protein (scFv-AP) was induced by IPTG.Its activity was detected by enzyme immunoassay. The molecular weights of scFv and scFv-AP were measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). RESULTS: The product of SOE PCR formed a band of 700 bp in agarose gel electrophoresis. SDS-PAGE demonstrated the molecular weight of scFv was 27 ku.Immunofluorescent assay (IFA) demonstrated its reactivity with TfR.The molecular weight of scFv-AP was 75 ku.Enzyme immunoassay showed that scFv-AP could specifically bind to human TfR and play AP activity. CONCLUSION: We have successfully prepared the anti-human TfR scFv and constructed the fusion protein of scFv and AP.It is promising for immunological experiments. 展开更多
关键词 Transferrin receptor Fusion protein Single chain Fv antibody Alkaline phosphatase Primary hepatocarcinoma
暂未订购 下载PDF
Study on The Method of Quantitative Analysis of Serum Ferritin and Soluble Transferrin Receptor with Protein Microarray Technology 认领 引用 被引量：5
2
作者 YIN Ji Yong SUN Jing +2 位作者 HUANG Jian LI Wen Xian HUO Jun Sheng 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2012年第4期430-439,共10页
Objective To establish and evaluate a protein serum ferritin （SF） and soluble transferrin receptor microarray method for combined measurement of （sTfR）. Methods Microarrayer was used to print both anti-SF antibodi... Objective To establish and evaluate a protein serum ferritin （SF） and soluble transferrin receptor microarray method for combined measurement of （sTfR）. Methods Microarrayer was used to print both anti-SF antibodies I and anti-sTfR antibodies I on each protein microarray. Anti-SF antibodies II and anti-sTfR antibodies II were used as detection antibodies and goat antibodies coupled to Cy3 were used as antibodies Ill. The detection conditions of the quantitative analysis method for simultaneous measurement of SF and sTfR with protein microarray were optimized and evaluated. The protein microarray was compared with commercially available traditional tests with 26 serum samples. Results By comparison experiment, mouse monoclonal antibodies were chosen as the probes and contact printing was chosen as the printing method. The concentrations of SF and sTfR probes were 0.5 mg/mL and 0.5 mg/mL respectively, while those of SF and sTfR detection antibodies were 5 μg/mL and 0.36 μg/mL respectively. Intra- and inter-assay variability was between 3.26% and 18.38% for all tests. The regression coefficients comparing protein microarray with traditional test assays were better than 0.81 for SF and sTfR. Conclusion The present study has established a protein microarray method for combined measurement of SF and sTfR. 展开更多
关键词 Protein microarray Optimization Combined measurement conditions Serum ferritin Soluble transferrin receptor.
暂未订购 下载PDF
Drug delivery via the transferrin receptor-mediated endocytosis pathway 认领 引用 被引量：2
3
作者 夏青 杨秀伟 +2 位作者 杨晓达 钱忠明 王夔 《Journal of Chinese Pharmaceutical Sciences》 CAS 2009年第1期7-13,共7页
The membrane transferrin receptor-mediated endocytosis has been exploited for developing novel targeted drug delivery systems, which could have a variety of applications in the site-specific delivery of anticancer dru... The membrane transferrin receptor-mediated endocytosis has been exploited for developing novel targeted drug delivery systems, which could have a variety of applications in the site-specific delivery of anticancer drugs, proteins and therapeutic genes into proliferating malignant cells that overexpress the transferrin receptors. This is achieved by coupling transferrin or monoclonal antibody to transferrin receptor with therapeutic drugs or drug delivery vesicles. The transferrin conjugates can be obtained by use of either bifunctional chemical linkers or by genetic infusion of therapeutic peptides/proteins into the structure of transferrin / and monoclonal antibody to transferrin receptor. A variety of drug carriers such as liposomes, nanoparticles and DNApolymer complexes （i.e. polyplex and lipoplex） were used to efficiently deliver the Wansferrin conjugates. Use of transferrin conjugates results in improvement in drug efficacy, selectivity and drug release as well as reduction in drug toxicity. This paper reviews the basic biochemistry of transferrin and the transferrin receptor as well as the strategy for developing targeted drug delivery system. 展开更多
关键词 Transferrin Transferrin receptor Drug delivery
暂未订购 下载PDF
Selection for Anti-transferrin Receptor Bispecific T-cell Engager in Different Molecular Formats 认领 引用
4
作者 Ming-peng FU Zi-long GUO +4 位作者 Hong-ling TANG Hui-fen ZHU Guan-xin SHEN Yong HE Ping LEI 《Current Medical Science》 SCIE CAS 2020年第1期28-34,共7页
Selecting an ideal molecular format from diverse structures is a major challenge in developing a bispecific antibody(BsAb).To choose an ideal format of anti-CD3 x anti-transferrin receptor(TfR)bispecific antibodies fo... Selecting an ideal molecular format from diverse structures is a major challenge in developing a bispecific antibody(BsAb).To choose an ideal format of anti-CD3 x anti-transferrin receptor(TfR)bispecific antibodies for clinical application,we constructed TfR bispecific T-cell engager(BiTE)in two extensively applied formats,including single-chain tandem singlechain variable fragments(scFvs)and double-chain diabodies,and evaluated their functional characterizations in vitro.Results demonstrated that TfR-BiTE in both formats directed potent killing of TfR+HepG2 cells.However,compared to two・chain diabodies,scFvs were more efficient in antigen binding and TfR target killing.Furthermore,different domain orders in scFvs would also be evaluated because single-TfR-CD3-His was preferable to single-CD3-TfR-His in immunotherapeutic strategies.Thus,the single-chain tandem TfR-CD3 format was favored for further investigation in cancer therapy. 展开更多
关键词 bispecific antibody single-chain tandem single-chain variable fragments diabody transferrin receptor CD3
暂未订购 下载PDF
Preparation and Identification of scFv and bsFv against Transferrin Receptor 认领 引用
5
作者 刘静 肖代雯 +7 位作者 周小鸥 文雪 代红 王志华 沈昕 代维 杨道锋 沈关心 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 2008年第6期621-625,共5页
To obtain single chain variable fragment （scFv） and bivalent single chain variable fragment （bsFv） against transferrin receptor, up-stream and down-stream primers were designed according to the complementary seque... To obtain single chain variable fragment （scFv） and bivalent single chain variable fragment （bsFv） against transferrin receptor, up-stream and down-stream primers were designed according to the complementary sequences of FR1 region of variable heavy （VH） and FR4 of variable light （VL）, respectively, which contained inter-linker G4S and the restriction endonuclease SfiI, AscI and NotI. Two pieces of scFv fragments were first amplified through PCR and then inserted into plasmid pAB1, which could express scFv protein once induced by IPTG in the host bacteria. To express scFv and bsFv, E. coli TG1 was cultured in LB broth and was induced by IPTG. The restriction enzyme digestion map and DNA sequencing demonstrated that scFv and bsFv genes were successfully inserted into the expression plasmid. SDS-PAGE and Western blotting revealed the protein band at 35kD and 60kD, which were consistent with the molecular weight of scFv and bsFv respectively. Flow cytometry showed that scFv and bsFv harbored the specific binding activity with TfR expressed in various tumor cells, and the avidity of bsFv was higher than that of the parent scFv. 展开更多
关键词 gene sequence G4S linker scFv bsFv transferrin receptor
暂未订购 下载PDF
Transferrin receptor and ferritin-H are developmentally regulated in oligodendrocyte lineage cells 认领 引用 被引量：7
6
作者 Yunxia Li Qiang Guan +3 位作者 Yuhui Chen Hongjie Han Wuchao Liu Zhiyu Nie 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第1期6-12,共7页
Iron is an essential trophic element that is required for cell viability and differentiation, especially in oligodendrocytes, which consume relatively high rates of energy to produce myelin. Multiple iron metabolism p... Iron is an essential trophic element that is required for cell viability and differentiation, especially in oligodendrocytes, which consume relatively high rates of energy to produce myelin. Multiple iron metabolism proteins are expressed in the brain including transferrin receptor and ferritin-H. However, it is still unknown whether they are developmentally regulated in oligodendrocyte lineage cells for myelination. Here, using an in vitro cultured differentiation model of oligodendrocytes, we found that both transferrin receptor and ferritin-H are significantly upregulated during oligodendrocyte maturation, implying the essential role of iron in the development of oligodendrocytes. Additional different doses of Fe3＋ in the cultured medium did not affect oligodendrocyte precursor cell maturation or ferritin-H expression but decreased the expression of the transferrin receptor. These results indicate that upregulation of both transferrin receptor and ferritin-H contributes to maturation and myelination of oligodendrocyte precursor cells. 展开更多
关键词 neural regeneration neurogenesis, oligodendrocyte iron transferrin receptor ferritin-H development myelinization proliferation induced differentiation grants-supported paper photographs-containing paper neuroregeneration
暂未订购 下载PDF
Cervical cancer with transferrin receptor has a poor prognosis and is associated with immune infiltration, according to a comprehensive bioinformatics study 认领 引用
7
作者 Dong-Mei Han Cai-Hong Wu +1 位作者 Bin Ling Hao Jin 《Cancer Advances》 2024年第6期1-9,共9页
Background:The molecular mechanism underlying the involvement of the Transferrin receptor(TFRC)in cervical cancer remains poorly understood.This study aims to elucidate the role of TFRC in cervical cancer by analyzing... Background:The molecular mechanism underlying the involvement of the Transferrin receptor(TFRC)in cervical cancer remains poorly understood.This study aims to elucidate the role of TFRC in cervical cancer by analyzing data from The Cancer Genome Atlas(TCGA)and Genotype-Tissue Expression(GTEx)databases.Methods:TFRC protein expression was obtained from Human Protein Altas(HPA).All datas were collected from TCGA and GTEx.In this study,we analyzed the expression of TFRC in cervical cancer and its clinical significance.Through Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene set enrichment analyses(GSEA),investigated the related molecular pathways of TFRC.The relationship between TFRC and immune infiltration was then examined.The prognosis of different immune cell subsets was then analyzed after dividing cervical cancer patients into high and low expression of TFRC groups.Results:TFRC is highly expressed in various tumor tissues compared to control normal tissues,including cervical cancer.An increased expression of TFRC was associated with higher Tumor(T)and Node(N)stage,as well as a higher clinical stage.Kaplan–Meier(KM)survival analysis investigated that higher TFRC expression patients have a poor overall survival(OS),disease specific survival(DSS)and progress free interval(PFI).Both KEGG and GSEA enriched signaling pathway by high TFRC and low TFRC groups.There was a significant negative linear correlation between TFRC expression and immune infiltration.TFRC affects the prognosis of cervical cancer patients through immune pathway.Conclusions:Cervical cancer patients with TFRC expression may have a worse prognosis. 展开更多
关键词 cervical cancer prognosis immune infiltration transferrin receptor
暂未订购 下载PDF
Effect of high flux hemodialysis on renal anemia and soluble transferrin receptor in hemodialysis patients 认领 引用
8
作者 Xiang-Geng Chi Wen-Bin Zhang +2 位作者 Qi Cai Yuan-Zhuan Chen De-Liang Ding 《Journal of Hainan Medical University》 CAS 2020年第13期39-42,共4页
Objective: To investigate the effect of high throughput hemodialysis on soluble transferrin receptor in hemodialysis patients and the improvement of renal anemia. Methods: 132 patients receiving maintenance hemodialys... Objective: To investigate the effect of high throughput hemodialysis on soluble transferrin receptor in hemodialysis patients and the improvement of renal anemia. Methods: 132 patients receiving maintenance hemodialysis in our hospital from July 2017 to July 2019 were selected and divided into control group and observation group according to the random number table method, with 66 cases each. The observation group was treated with high-flux hemodialysis, while the control group was treated with low-flux hemodialysis for 6 months. Compare two groups before and after treatment serum beta 2 microglobulin (beta 2 - MG), serum creatinine (Scr), blood urea nitrogen (BUN) level, anemia related index [red blood cells deposited (HCT), hemoglobin (Hb), reticulocyte percentage (Ret%)], iron metabolism index [serum ferritin (SF), transferrin saturation (TSAT)、Hepcidin(Hepc)], soluble transferrin receptor (sTfR) levels and adverse reactions. Results: the levels of 2-MG, Scr and BUN in the two groups before treatment were compared (P>0.05). After treatment, Scr and BUN levels in the two groups were significantly decreased (P0.05). The level of 2-MG in the observation group was lower than that in the control group (P0.05). After treatment, Hb and HCT levels in the observation group were higher than those in the control group, while Ret% were lower than those in the control group, (P0.05). After treatment, the level of ST and TAST in the observation group was higher than that in the control group, The levels of sTfR and Hepc were lower than the control group (P0.05). Conclusion: The high-throughput hemodialysis department significantly improved renal anemia in hemodialysis patients, reduced serum sTfR level, and had fewer adverse reactions and higher safety. 展开更多
关键词 High-throughput hemodialysis Renal anemia Soluble transferrin receptor
暂未订购 下载PDF
Role of transferrin receptor in hepatitis C viral infection 认领 引用
9
作者 Quan Liang 《国际感染病学（电子版）》 2018年第2期33-37,共5页
Hepatitis C virus(HCV) is the main pathogen causing chronic hepatitis and primary liver cancer. Various viral proteins and host cell molecules are involved in the HCV cell entry, but the mechanism of infection has not... Hepatitis C virus(HCV) is the main pathogen causing chronic hepatitis and primary liver cancer. Various viral proteins and host cell molecules are involved in the HCV cell entry, but the mechanism of infection has not been completely elucidated. The transferrin receptor can act as a receptor for many viruses during cell entry. The transferrin receptor is not only closely related to HCV-induced iron metabolism disorders but also mediates the fusion of HCV with the host cell membrane as a specific receptor for CD81-dependent viral adhesion. 展开更多
关键词 丙型肝炎病 患者 治疗方法 临床分析
暂未订购 下载PDF
Transferrin receptor 1 nuclear translocation facilitates tumor progression via p53-mediated chromatin interactions and genome-wide alterations 认领 引用 被引量：2
10
作者 Yaxin Hou Guoheng Tang +8 位作者 Qizhi Wang Meng Zhou Ran Xu Xuehui Chen Guizhi Shi Zhuoran Wang Xiyun Yan Jie Zhuang Kelong Fan 《Signal Transduction and Targeted Therapy》 SCIE CSCD 2025年第8期4508-4522,共15页
Transferrin receptor 1(TfR1),a widely expressed type Ⅱ transmembrane glycoprotein located on the plasma membrane,is well known for its established role in cellular iron uptake.Nevertheless,emerging evidence implies t... Transferrin receptor 1(TfR1),a widely expressed type Ⅱ transmembrane glycoprotein located on the plasma membrane,is well known for its established role in cellular iron uptake.Nevertheless,emerging evidence implies that TfR1 exhibits previously unrecognized noncanonical functions.Herein,we demonstrated the nuclear translocation of TfR1 and revealed the interaction between TfR1 and p53 within the nucleus.Through comprehensive analyses at the proteomic,genomic,and transcriptomic levels,we demonstrated that this interaction significantly influences the transcriptional activity of p53 on its downstream target genes,which are highly enriched in DNA damage repair functions.Specifically,our investigation revealed the indispensable role of nuclear TfR1 in the regulation of the nucleotide excision repair(NER)pathway,exemplified by the transcriptional regulation of XPC. 展开更多
关键词 transferrin receptor tfr plasma membraneis nuclear translocation transferrin receptor typeⅡtransmembrane glycoprotein comprehensive analyses tumor progression
暂未订购 下载PDF
Transferrin receptor-targeted immunostimulant for photodynamic immunotherapy against metastatic tumors through β-catenin/CREB interruption 认领 引用 被引量：1
11
作者 Mengyi Yan Xiayun Chen +9 位作者 Xiaotong Li Qianqian Liu Baixue Yu Yi Cen Wei Zhang Yibin Liu Xinxuan Li Ying Chen Tao Wang Shiying Li 《Acta Pharmaceutica Sinica B》 SCIE CAS CSCD 2024年第9期4118-4133,共16页
The immunosuppressive phenotype of tumor cells extensively attenuates the immune activation effects of traditional treatments.In this work,a transferrin receptor(TfR)targeted immunostimulant(PTI)is fabricated for phot... The immunosuppressive phenotype of tumor cells extensively attenuates the immune activation effects of traditional treatments.In this work,a transferrin receptor(TfR)targeted immunostimulant(PTI)is fabricated for photodynamic immunotherapy against metastatic tumors by interrupting β-catenin signal pathway.To synthesize PTI,the photosensitizer conjugated TfR targeting peptide moiety(Palmitic-K(PpIX)-HAIYPRH)is unitized to encapsulate the transcription interrupter of ICG-001.On the one hand,the recognition of PTI and TfR can promote drug delivery into tumor cells to destruct primary tumors through photodynamic therapy and initiate an immunogenic cell death with the release of tumorassociated antigens.On the other hand,PTI will interrupt the binding between b-catenin andcAMP response element-binding protein(CREB),regulating the gene transcription to downregulate programmed death ligand 1(PD-L1)while upregulating CeC motif chemokine ligand 4(CCL4).Furthermore,the elevated CCL4 can recruit the dendritic cells to present tumor-specific antigens and promote T cells activation and infiltration,and the downregulated PD-L1 can avoid the immune evasion of tumor cells and activate systemic anti-tumor immunity to eradicate lung metastasis.This work may inspire the development of antibody antibody-free strategy to activate systemic immune response in consideration of immunosuppressive conditions. 展开更多
关键词 Transferrin receptor β-Catenin signal pathway Tumor targeting Photodynamic therapy Immunotherapy Immunogenic cell death Programmed death ligand 1 C-C motif chemokine ligand 4
暂未订购 下载PDF
Transferrin receptor and Fc α/μ receptor may not be the major IgA_1 receptor on human mesangial cells 认领 引用 被引量：2
12
作者 HURui-hai ZHANGYing ZHAOMing-hui 《Chinese Medical Journal》 SCIE CAS 2005年第9期781-785,共5页
IgA nephropathy (IgAN) is the most common form of primary glomerulonephritis. 1 The histopathology of IgAN is characterized by abundance of mesangial matrix and proliferation of mesangial cells. IgA_1 deposition in th... IgA nephropathy (IgAN) is the most common form of primary glomerulonephritis. 1 The histopathology of IgAN is characterized by abundance of mesangial matrix and proliferation of mesangial cells. IgA_1 deposition in the mesangium plays an important role in the inflammatory process in this disease. 展开更多
关键词 immunoglobulin A . IgA nephropathy . mesangial cell . transferrin receptor . Fc α/μ receptor
Ultraviolet-B induced expression of hypoxia-inducible factor 1α, transferrin receptor through EGFR/PI3K/AKT/DEC1 pathway 认领 引用
13
作者 LI Yanhua BI Zhigang 《Frontiers of Medicine》 2007年第1期79-86,共8页
The aim of this research was to explore the effects and signaling pathway of ultraviolet-B(UVB)irradiation on the expression of hypoxia-inducible factor 1a(HIF-1α)and transferrin receptor(TfR).HIF-1α protein was mea... The aim of this research was to explore the effects and signaling pathway of ultraviolet-B(UVB)irradiation on the expression of hypoxia-inducible factor 1a(HIF-1α)and transferrin receptor(TfR).HIF-1α protein was measured by Western blot method.Expressions of epidermal growth factor receptor(EGFR),phosphor-EGF-R and TfR after UVB irradiation were determined with flow cytometry.After UVB irradiation,mRNA levels of HIF-1α and TfR were detected by real time-PCR.Results showed that compared with control groups,UVB was able to induce HIF1α and TfR protein expression in a dose-and time-dependent manner in HaCat cells(P0.05).The EGFR/PI3K/AKT signaling pathway was required for the induction of HIF-1α and TfR expression induced by UVB.UVB induced activation of EGFR in HaCat cells and EGFR regulated expression of TfR and HIF-1α.EGFR(−/−)MEF did not increase the HIF1 expression following UVB irradiation(P>0.05).In contrast,EGFR(+/+)MEF strongly enhanced HIF1a expression after UVB irradiation(P0.05).In contrast,DEC1(+/+)HaCat cells strongly enhanced TfR and HIF-1α protein expression after UVB irradiation(P<0.05).We conclude that UVB induces TfR and HIF-1α expressions via EGFR/PI3K/AKT/DEC1 signaling pathway. 展开更多
关键词 ultraviolet-B hypoxia-inducible factor 1α receptors,transferrin receptors,epidermal growth factor phosphatidylinositol-3-kinase DEC1
暂未订购 下载PDF
N,N-二甲基甲酰胺接触激活固醇调节元件结合蛋白并影响铁代谢 认领 引用
14
作者 蒋晓霞 吴梅 +4 位作者 陈祝军 吴建兵 陈蓉 沈佳怡 李志君 《中国职业医学》 CAS 2026年第1期79-83,91,共5页
目的 探讨职业性接触N,N-二甲基甲酰胺(DMF)对工人体内固醇调节元件结合蛋白(SREBP)与铁代谢指标的影响。方法 采用目的抽样法,选择职业接触DMF的20名工人为接触组,以同厂不接触DMF的15名健康工人为对照组。采用个体采样法,检测研究对象... 目的 探讨职业性接触N,N-二甲基甲酰胺(DMF)对工人体内固醇调节元件结合蛋白(SREBP)与铁代谢指标的影响。方法 采用目的抽样法,选择职业接触DMF的20名工人为接触组,以同厂不接触DMF的15名健康工人为对照组。采用个体采样法,检测研究对象DMF接触水平;采用气相色谱法检测尿甲基甲酰胺(NMF)水平;采用实时荧光定量聚合酶链反应法检测周围血中SREBP-1a、SREBP-1c、SREBP-2的mRNA相对表达水平,采用酶联免疫吸附法检测血清中转铁蛋白、可溶性转铁蛋白受体(sTfR)与铁蛋白水平。结果 对照组人群DMF接触水平均低于检测限,尿NMF均未检出。接触组人群DMF接触水平中位数为13.30 mg/m3,尿NMF水平平均为(9.72±1.78) mg/g·Cr。与对照组比较,接触组人群周围血中SREBP-1a、SREBP-1c和SREBP-2的mRNA相对表达水平均增加(P值均0.05)。接触组人群尿NMF水平与周围血中SREBP-1c的mRNA相对表达水平呈负相关[相关系数(r)=-0.50,P=0.03],与血清中转铁蛋白、sTfR和铁蛋白水平均呈正相关(r分别为0.63、0.58、0.52,P值均<0.05)。结论 职业性DMF接触可激活SREBP家族,上调其mRNA表达,并干扰铁代谢,两者存在一定关联。 展开更多
关键词 二甲基甲酰胺 固醇调节元件结合蛋白 转铁蛋白 可溶性转铁蛋白受体 铁蛋白
暂未订购 下载PDF
维持性血液透析患者中测定血清可溶性转铁蛋白受体和铁蛋白指数的临床价值探讨 认领 引用
15
作者 李秀季 张梅 +1 位作者 陈燊 张国娟 《中国医药导报》 CAS 2026年第7期66-72,共7页
目的探讨维持性血液透析(MHD)患者中测定血清可溶性转铁蛋白受体(sTfR)和铁蛋白指数在评估铁缺乏中的临床应用价值。方法选取2022年6月首都医科大学附属北京同仁医院血液透析中心稳定MHD患者91例,记录患者一般资料、血常规、生化、铁代... 目的探讨维持性血液透析(MHD)患者中测定血清可溶性转铁蛋白受体(sTfR)和铁蛋白指数在评估铁缺乏中的临床应用价值。方法选取2022年6月首都医科大学附属北京同仁医院血液透析中心稳定MHD患者91例,记录患者一般资料、血常规、生化、铁代谢指标、重组人红细胞生成素(rHuEPO)剂量及补铁治疗情况。根据sTfR中位数将患者分为低sTfR组(47例)和高sTfR组(44例)。统计分析两组临床资料、化验指标及用药情况的差异。对sTfR和Ln铁蛋白指数与各项指标进行相关分析。采用多元线性回归分析确定sTfR和Ln铁蛋白指数的影响因素。通过绘制受试者操作特征(ROC)曲线分析sTfR和铁蛋白指数对诊断铁缺乏的预测价值。结果高sTfR组患者的透析龄、尿素清除指数、总铁结合力(TIBC)、转铁蛋白、sTfR、铁蛋白指数、rHuEPO剂量及未用铁剂治疗患者比例高于低sTfR组(P<0.05),而男性比例、平均红细胞血红蛋白含量(MCH)、平均红细胞血红蛋白浓度(MCHC)、转铁蛋白饱和度(TSAT)低于低sTfR组(P<0.05)。MHD患者sTfR与女性、透析龄、血红蛋白、hsCRP、iPTH、TIBC、转铁蛋白及rHuEPO剂量呈正相关(r=0.231、0.390、0.233、0.222、0.259、0.580、0.559、0.237,P<0.05),与MCH、MCHC、血清铁、TSAT及应用铁剂治疗呈负相关(r=-0.293、-0.407、-0.239、-0.442、-0.326,P<0.05)。Ln铁蛋白指数与血红蛋白、iPTH、TIBC、转铁蛋白和rHuEPO剂量呈正相关(r=0.301、0.285、0.701、0.727、0.246,P<0.05),与MCH、MCHC、血清铁、TSAT、血清铁蛋白及应用铁剂治疗呈负相关(r=-0.365、-0.393、-0.318、-0.583、-0.728、-0.237,P<0.05)。多元线性回归分析结果显示,透析龄、转铁蛋白、rHuEPO剂量与sTfR呈正相关(P<0.05)。透析龄、转铁蛋白与Ln铁蛋白指数呈正相关(P<0.05)。ROC曲线显示,sTfR诊断铁缺乏的曲线下面积(AUC)为0.719(95%CI:0.604~0.834),截断值为16.25 nmol/L,灵敏度和特异度分别为95.5%和46.4%;铁蛋白指数的AUC为0.898(95%CI:0.830~0.966),截断值为10.54,灵敏度和特异度分别为86.4%和87.0%。结论MHD患者中铁蛋白指数在评估铁缺乏方面优于sTfR,可能与sTfR水平受炎症、rHuEPO治疗的影响有关。 展开更多
关键词 血液透析 可溶性转铁蛋白受体 铁蛋白指数 铁缺乏
暂未订购 下载PDF
槲皮素通过TFRC调控脓毒症心肌病小鼠和心肌细胞线粒体损伤和铁死亡 认领 引用
16
作者 王晔 郝丽 +3 位作者 李京辉 王铭 符曼昱 付莉 《昆明医科大学学报》 CAS 2026年第8期13-27,共15页
目的探讨槲皮素(quercetin,QUE)调节转铁蛋白受体(transferrin receptor,TFRC)对脓毒症心肌病模型细胞线粒体和铁死亡的影响并阐明相关调节机制。方法利用1μg/mL脂多糖(lipopolysaccharide,LPS)分别与20μM、40μM、80μM、160μM QUE... 目的探讨槲皮素(quercetin,QUE)调节转铁蛋白受体(transferrin receptor,TFRC)对脓毒症心肌病模型细胞线粒体和铁死亡的影响并阐明相关调节机制。方法利用1μg/mL脂多糖(lipopolysaccharide,LPS)分别与20μM、40μM、80μM、160μM QUE培养H9C2细胞24 h。在80μM QUE处理的H9C2细胞中转染shTFRC、pcDNA-TFRC或用铁死亡抑制剂Ferrostatin-1处理24 h。通过CCK-8,流式细胞术,JC-1染色,Western blot,Fe2+、LDH和MDA试剂盒,酶联免疫吸附和RT-qPCR实验探讨QUE对TFRC表达的调控以及QUE调控TFRC对LPS诱导H9C2细胞线粒体功能和铁死亡的影响。QUE经腹腔注射至小鼠并经LPS诱导SCM小鼠模型,经HE染色,TUNEL染色,ELISA,LDH和MDA试剂盒以及Western blot实验探讨QUE对SCM小鼠的影响。结果LPS诱导可抑制H9C2细胞增殖,促进细胞凋亡、ROS、Cleaved-caspase 3、Bax、FTH1、ACSL4、Fe2+、白介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子(tumor necrosis factor-α,TNF-α)、乳酸脱氢酶(lactate dehydrogenase,LDH)和肌酸激酶同工酶MB(creatine kinase-MB,CK-MB)水平(P<0.001),降低线粒体膜电位和Bcl-2、谷胱甘肽过氧化物酶4(glutathione peroxidase 4,GPX4)蛋白表达(P<0.001);经40μM、80μM、160μM QUE处理可减轻LPS诱导的H9C2细胞线粒体功能障碍和铁死亡(P<0.05),抑制TFRC表达(P<0.001);敲低TFRC或经Ferrostatin-1处理可进一步增强80μM QUE对H9C2细胞损伤的保护作用(P<0.05)。过表达TFRC可增强LPS诱导的H9C2细胞线粒体损伤和铁死亡,QUE处理可缓解过表达TFRC对H9C2的作用(P<0.05)。LPS诱导的SCM小鼠心肌细胞损伤和死亡增加、炎性细胞因子水平增加(P<0.05);经QUE处理的SCM小鼠心肌损伤缓解,铁死亡被抑制(P<0.05)。结论QUE通过下调TFRC表达,抑制铁死亡,缓解LPS诱导的H9C2细胞线粒体损伤以及小鼠心肌损伤。 展开更多
关键词 脓毒症心肌病 槲皮素 转铁蛋白受体 线粒体 铁死亡
暂未订购 下载PDF
黄芪多糖通过Hepcidin-FPN1轴调节铁代谢改善妊娠期缺铁性贫血的机制研究 认领 引用
17
作者 张小敏 李智慧 朱娴 《中国优生与遗传杂志》 2026年第2期266-271,共6页
目的探究黄芪多糖(APS)对妊娠期大鼠铁缺乏症状的改善及铁代谢作用,并初步探索其作用机制。方法50只未孕雌鼠随机均分对照组、模型组、APS低剂量组、APS高剂量组、琥珀酸亚铁组;除对照组外,其余组建立妊娠期IDA模型。自孕11天起,每日灌... 目的探究黄芪多糖(APS)对妊娠期大鼠铁缺乏症状的改善及铁代谢作用,并初步探索其作用机制。方法50只未孕雌鼠随机均分对照组、模型组、APS低剂量组、APS高剂量组、琥珀酸亚铁组;除对照组外,其余组建立妊娠期IDA模型。自孕11天起,每日灌胃给药,持续至产后21天。生化法检测血液学指标;酶联免疫吸附法检测血清铁代谢指标及组织中铁含量;苏木精-伊红染色观察肝脏组织细胞形态;免疫组化法观察肝脏组织铁调素(Hepcidin)、膜铁转运蛋白1(FPN1)蛋白分布及表达;Western blot法检测肝脏组织转铁蛋白受体(TfR)1、TfR2、Hepcidin、FPN1蛋白表达。结果与对照组比较,模型组大鼠血红蛋白(Hb)、血细胞比容(HCT)、红细胞(RBC)、平均红细胞血红蛋白浓度(MCHC),血清铁(SI)、总铁结合力(TIBC)、转铁蛋白饱和度(TSAT),肝脏、心脏、脾脏组织中铁蛋白含量均降低(P<0.05);肝脏组织中TfR1、TfR2、FPN1蛋白水平降低,Hepcidin蛋白水平升高(P<0.05),且组织病理重度损伤。与模型组比较,APS低剂量组、APS高剂量组和琥珀酸亚铁组大鼠Hb、HCT、RBC、MCHC,血清SI、TIBC、TSAT,肝脏、心脏、脾脏组织中铁蛋白含量均升高(P<0.05);肝脏组织中TfR1、TfR2、FPN1蛋白水平升高,Hepcidin蛋白水平降低(P<0.05),且组织病理轻度损伤。APS对妊娠期IDA大鼠的改善作用呈剂量依赖性(P<0.05),且高剂量的APS与琥珀酸亚铁效果相当(P<0.05)。结论APS可改善妊娠期IDA大鼠铁缺乏,调节铁代谢,其作用机制可能与调控Hepcidin-FPN1轴相关。 展开更多
关键词 黄芪多糖 妊娠期缺铁性贫血 铁代谢 转铁蛋白受体 Hepcidin-FPN1轴
暂未订购 下载PDF
抗磷脂酶A2受体抗体与尿液蛋白标志物联合检测在原发性膜性肾病诊断中的价值 认领 引用
18
作者 王文涓 金纪之 +1 位作者 张春旭 刘华 《检验医学》 CAS 2026年第6期560-565,共6页
目的探讨血清抗磷脂酶A2受体抗体(PLA2R-Ab)和尿液蛋白标志物联合检测在原发性膜性肾病(PMN)诊断中的价值。方法选取2023年7月—2024年3月上海交通大学附属第六人民医院PMN患者61例(PMN组)、IgA肾病(IgAN)患者49例(IgAN组)、糖尿病肾病(... 目的探讨血清抗磷脂酶A2受体抗体(PLA2R-Ab)和尿液蛋白标志物联合检测在原发性膜性肾病(PMN)诊断中的价值。方法选取2023年7月—2024年3月上海交通大学附属第六人民医院PMN患者61例(PMN组)、IgA肾病(IgAN)患者49例(IgAN组)、糖尿病肾病(DN)患者156例(DN组)、其他肾小球疾病患者42例(其他组),同时检测血清PLA2R-Ab、IgG4和尿液α1-微球蛋白(α1-MG)、IgG、转铁蛋白(TF)、微量白蛋白(mAlb)、肌酐(Cr)、红细胞(RBC)计数以及尿蛋白,计算尿白蛋白/肌酐比值(ACR)、24 h尿mAlb和24 h尿蛋白。采用Spearman相关分析评估PLA2R-Ab与肾功能指标的相关性。采用Logistic回归分析评估PMN发生的影响因素。采用受试者工作特征(ROC)曲线评价各项指标诊断PMN的效能。结果PMN组、IgAN组、DN组和其他组之间血清PLA2R-Ab、尿mAlb、尿TF、尿IgG、ACR和尿RBC计数差异均有统计学意义(P0.05)。在PLA2R-Ab阳性的PMN患者中,血清PLA2R-Ab水平与ACR、尿IgG呈正相关(r值分别为0.239、0.228,P<0.05)。尿IgG、尿TF和血清PLA2R-Ab是PMN发生的独立危险因素[比值比(OR)值分别为0.994、1.012、1.358,95%可信区间(CI)分别为0.990~0.999、1.006~1.017、1.039~1.774,P<0.05]。尿IgG、尿TF、血清PLA2R-Ab单项检测和联合检测诊断PMN的AUC分别为0.650、0.814、0.858和0.953。结论血清PLA2R-Ab、尿IgG和尿TF与PMN发生有关,三者联合检测可显著提升PMN的诊断效能。 展开更多
关键词 抗磷脂酶A2受体抗体 转铁蛋白 免疫球蛋白G 尿液 原发性膜性肾病
暂未订购 下载PDF
Eph receptor A4 regulates motor neuron ferroptosis in spinal cord ischemia/reperfusion injury in rats 认领 引用 被引量：4
19
作者 Yan Dong Chunyu Ai +5 位作者 Ying Chen Zaili Zhang Dong Zhang Sidan Liu Xiangyi Tong Hong Ma 《Neural Regeneration Research》 SCIE CAS CSCD 2023年第10期2219-2228,共10页
Previous studies have shown that the receptor tyrosine kinase Eph receptor A4(EphA4) is abundantly expressed in the nervous system. The EphA4 signaling pathway plays an important role in regulating motor neuron ferrop... Previous studies have shown that the receptor tyrosine kinase Eph receptor A4(EphA4) is abundantly expressed in the nervous system. The EphA4 signaling pathway plays an important role in regulating motor neuron ferroptosis in motor neuron disease. To investigate whether EphA4 signaling is involved in ferroptosis in spinal cord ischemiaeperfusion injury, in this study we established a rat model of spinal cord ischemiaeperfusion injury by clamping the left carotid artery and the left subclavian artery. We found that spinal cord ischemiaeperfusion injury increased EphA4 expression in the neurons of anterior horn, markedly worsened ferroptosis-related indicators, substantially increased the number of mitochondria exhibiting features consistent with ferroptosis, promoted deterioration of motor nerve function, increased the permeability of the blood-spinal cord barrier, and increased the rate of motor neuron death. Inhibition of EphA4 largely rescued these effects. However, intrathecal administration of the ferroptosis inducer Erastin counteracted the beneficial effects conferred by treatment with the EphA4 inhibitor. Mass spectrometry and a PubMed search were performed to identify proteins that interact with EphA4, with the most notable being Beclin1 and Erk1/2. Our results showed that inhibition of EphA4 expression reduced binding to Beclin1, markedly reduced p-Beclin1, and reduced Beclin1-XCT complex formation. Inhibition of EphA4 also reduced binding to p-Erk1/2 and markedly decreased the expression of c-Myc, transferrin receptor 1, and p-Erk1/2. Additionally, we observed co-localization of EphA4 and p-Beclin1 and of EphA4 and p-ERK1/2 in neurons in the anterior horn. In conclusion, EphA4 participates in regulating ferroptosis of spinal motor neurons in the anterior horn in spinal cord ischemiaeperfusion injury by promoting formation of the Beclin1-XCT complex and activating the Erk1/2/c-Mycransferrin receptor 1 axis. 展开更多
关键词 Beclin1 c-Myc EphA4 Erk1/2 ferroptosis motor neuron p-Erk1/2 rat spinal cord ischemiaeperfusion injury transferrin receptor 1
暂未订购 下载PDF
铁蛋白指数在炎症状态下对血液透析肾性贫血患者铁缺乏的诊断价值 认领 引用 被引量：1
20
作者 李万有 刘璐 +2 位作者 肖洋 李西超 王松岩 《中国实验诊断学》 2026年第2期218-222,共5页
目的探讨铁蛋白指数(sTfR/logSF)在炎症状态下对血液透析肾性贫血患者铁缺乏的诊断价值。方法选择2025年6月至2025年10月于吉林省人民医院血液净化中心行规律血液透析的肾性贫血患者共121例为研究对象,分为超敏-C反应蛋白(hs-CRP)升高组... 目的探讨铁蛋白指数(sTfR/logSF)在炎症状态下对血液透析肾性贫血患者铁缺乏的诊断价值。方法选择2025年6月至2025年10月于吉林省人民医院血液净化中心行规律血液透析的肾性贫血患者共121例为研究对象,分为超敏-C反应蛋白(hs-CRP)升高组(n=61)和hs-CRP正常组(n=60),检测血常规、血清铁(SI)、总铁结合力(TIBC)、铁蛋白(SF)、转铁蛋白饱和度(TSAT)、血白蛋白(Alb)、hs-CRP及可溶性转铁蛋白受体(sTfR),计算sTfR/logSF;比较两组患者各指标组间的差异,评估sTfR/logSF的影响因素;采用受试者工作特征曲线(ROC)来评估sTfR/logSF在炎症状态下对铁缺乏的诊断价值。结果hs-CRP升高组的sTfR和sTfR/logSF与hs-CRP正常组比较差异无统计学意义(P>0.05),hs-CRP升高组的TSAT、SI及Alb低于hs-CRP正常组(P0.05)。结论sTfR/logSF在炎症状态下对血液透析肾性贫血患者铁缺乏具有较高的诊断价值,可用于评估伴随炎症状态的血液透析肾性贫血患者铁缺乏。 展开更多
关键词 铁蛋白指数 可溶性转铁蛋白受体 血液透析 肾性贫血 铁缺乏
暂未订购 下载PDF
已选0条
引用分析
参考文献
引证文献
 统计分析
检索结果
已选文献
上一页 1 2 … 17 下一页 到第 页
在线咨询 使用帮助 返回顶部 意见反馈